SitesBLAST
Comparing Pf6N2E2_2611 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2611 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2gnaA Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-gal (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 3:328/329 of 2gnaA
- binding galactose-uridine-5'-diphosphate: K87 (= K85), S176 (= S174), V177 (= V175), T195 (= T193), M199 (= M197), R201 (= R199), M235 (≠ I233), R254 (= R252), E257 (= E255)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), T15 (= T13), G16 (= G14), S17 (= S15), F18 (= F16), S39 (= S37), R40 (= R38), D41 (= D39), K44 (= K42), D63 (= D61), V64 (= V62), A83 (= A81), A84 (= A82), A85 (= A83), K87 (= K85), L125 (= L123), S126 (= S124), Y137 (= Y135), K141 (= K139), Y167 (= Y165), G168 (= G166), V170 (= V168)
2gn9A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glc (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 3:328/329 of 2gn9A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), T15 (= T13), G16 (= G14), S17 (= S15), F18 (= F16), R40 (= R38), D41 (= D39), K44 (= K42), D63 (= D61), V64 (= V62), A83 (= A81), A84 (= A82), A85 (= A83), K87 (= K85), L125 (= L123), S126 (= S124), K141 (= K139), Y167 (= Y165), G168 (= G166), V170 (= V168), R174 (= R172)
- binding uridine-5'-diphosphate-glucose: K87 (= K85), T127 (= T125), K129 (= K127), Y137 (= Y135), N169 (= N167), S176 (= S174), V177 (= V175), P193 (= P191), T195 (= T193), M199 (= M197), R201 (= R199), M235 (≠ I233), R254 (= R252), E257 (= E255)
2gn6A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp-glcnac (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 3:328/329 of 2gn6A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), T15 (= T13), G16 (= G14), S17 (= S15), F18 (= F16), R40 (= R38), D41 (= D39), K44 (= K42), D63 (= D61), V64 (= V62), A84 (= A82), A85 (= A83), K87 (= K85), S126 (= S124), Y137 (= Y135), K141 (= K139), Y167 (= Y165), G168 (= G166), V170 (= V168), S173 (= S171), R174 (= R172)
- binding uridine-diphosphate-n-acetylglucosamine: K87 (= K85), D128 (= D126), K129 (= K127), N169 (= N167), G175 (= G173), S176 (= S174), V177 (= V175), P193 (= P191), I194 (= I192), M199 (= M197), R201 (= R199), M235 (≠ I233), R254 (= R252), E257 (= E255)
2gn4A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADPH and udp-glcnac (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 3:328/329 of 2gn4A
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G13 (= G11), T15 (= T13), G16 (= G14), S17 (= S15), F18 (= F16), S39 (= S37), R40 (= R38), D41 (= D39), K44 (= K42), D63 (= D61), V64 (= V62), A83 (= A81), A84 (= A82), A85 (= A83), K87 (= K85), T102 (= T100), L125 (= L123), S126 (= S124), T127 (= T125), Y137 (= Y135), K141 (= K139), Y167 (= Y165), G168 (= G166), V170 (= V168), S173 (= S171), R174 (= R172)
- binding uridine-diphosphate-n-acetylglucosamine: K87 (= K85), T127 (= T125), D128 (= D126), K129 (= K127), Y137 (= Y135), N169 (= N167), S176 (= S174), V177 (= V175), P193 (= P191), T195 (= T193), M199 (= M197), R201 (= R199), M235 (≠ I233), R254 (= R252), E257 (= E255)
2gn8A Crystal structure of udp-glcnac inverting 4,6-dehydratase in complex with NADP and udp (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 1:326/327 of 2gn8A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), T13 (= T13), G14 (= G14), S15 (= S15), F16 (= F16), S37 (= S37), R38 (= R38), D39 (= D39), K42 (= K42), D61 (= D61), V62 (= V62), A81 (= A81), A82 (= A82), A83 (= A83), K85 (= K85), T100 (= T100), L123 (= L123), S124 (= S124), K139 (= K139), Y165 (= Y165), G166 (= G166), V168 (= V168), S171 (= S171), R172 (= R172)
- binding uridine-5'-diphosphate: K127 (= K127), N167 (= N167), V175 (= V175), P191 (= P191), I192 (= I192), T193 (= T193), M197 (= M197), R199 (= R199), M233 (≠ I233), R252 (= R252)
O25511 UDP-N-acetylglucosamine 4,6-dehydratase (inverting); Pseudaminic acid biosynthesis protein B; UDP-GlcNAc-inverting 4,6-dehydratase; EC 4.2.1.115 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
58% identity, 98% coverage: 1:327/333 of query aligns to 7:332/333 of O25511
- TGSF 19:22 (= TGSF 13:16) binding NADP(+)
- SRDELK 43:48 (= SRDELK 37:42) binding NADP(+)
- DV 67:68 (= DV 61:62) binding NADP(+)
- A87 (= A81) binding NADP(+)
- K91 (= K85) binding NADP(+)
- LS 129:130 (= LS 123:124) binding NADP(+)
- K133 (= K127) mutation K->A,E: Loss of activity.
- Y141 (= Y135) binding NADP(+)
- K145 (= K139) binding NADP(+)
- VVGSR 174:178 (≠ VAGSR 168:172) binding NADP(+)
3w1vA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with inihibitor (see paper)
39% identity, 98% coverage: 2:326/333 of query aligns to 11:331/347 of 3w1vA
- binding [(2R,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,4-bis(oxidanyl)oxan-2-yl]methylimino-azanylidene-azanium: K93 (= K85), Q94 (= Q86), N175 (= N167), S179 (= S171), R180 (= R172), S182 (= S174), V183 (= V175), L186 (≠ F178), T198 (≠ P191), I199 (= I192), T200 (= T193), M204 (= M197), R206 (= R199), V240 (≠ I233), R263 (= R252), E266 (= E255)
4g5hA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in complex with by-product (see paper)
39% identity, 98% coverage: 2:326/333 of query aligns to 11:331/346 of 4g5hA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G11), T22 (= T13), G23 (= G14), S24 (= S15), F25 (= F16), S45 (= S37), R46 (= R38), D47 (= D39), K50 (= K42), D69 (= D61), V70 (= V62), A89 (= A81), A90 (= A82), A91 (= A83), K93 (= K85), L131 (= L123), T133 (= T125), K147 (= K139), Y173 (= Y165)
- binding [(2R,3R,4R,6R)-3-acetamido-6-methyl-4-oxidanyl-5-oxidanylidene-oxan-2-yl] [[(2R,3S,4R,5R)-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Q94 (= Q86), V95 (= V87), K135 (= K127), N175 (= N167), S182 (= S174), V183 (= V175), L186 (≠ F178), T198 (≠ P191), T200 (= T193), M204 (= M197), V240 (≠ I233), R263 (= R252), E266 (= E255), Y278 (≠ A267), S313 (≠ D307), Y314 (≠ F308), E315 (= E309), Y316 (= Y310), N320 (= N315)
3vvcA Crystal structure of capsular polysaccharide synthesizing enzyme cape , k126e, in apo form (see paper)
38% identity, 98% coverage: 2:326/333 of query aligns to 3:303/318 of 3vvcA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), T14 (= T13), G15 (= G14), F17 (= F16), S37 (= S37), R38 (= R38), D39 (= D39), K42 (= K42), D61 (= D61), V62 (= V62), R63 (= R63), A81 (= A81), A82 (= A82), A83 (= A83), K85 (= K85), S124 (= S124), T125 (= T125), K139 (= K139), Y165 (= Y165), G166 (= G166)
6bwcC X-ray structure of pen from bacillus thuringiensis (see paper)
36% identity, 98% coverage: 2:327/333 of query aligns to 3:319/327 of 6bwcC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), T14 (= T13), G15 (= G14), T16 (≠ S15), I17 (≠ F16), S37 (= S37), R38 (= R38), S39 (≠ D39), D63 (= D61), I64 (≠ V62), V83 (≠ A81), A84 (= A82), K87 (= K85), T125 (≠ L123), S127 (≠ T125), Y137 (= Y135), K141 (= K139), F167 (≠ Y165), V170 (= V168), S173 (= S171), R174 (= R172)
- binding uridine-diphosphate-n-acetylglucosamine: K87 (= K85), H88 (≠ Q86), S127 (≠ T125), N128 (≠ D126), Y137 (= Y135), N169 (= N167), S176 (= S174), V177 (= V175), L180 (≠ F178), T192 (≠ P191), T194 (= T193), M198 (= M197), R200 (= R199), L234 (≠ I233), E265 (= E255)
4j2oC Crystal structure of NADP-bound wbjb from a. Baumannii community strain d1279779 (see paper)
42% identity, 84% coverage: 1:279/333 of query aligns to 1:282/316 of 4j2oC
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), T13 (= T13), G14 (= G14), S15 (= S15), F16 (= F16), S36 (= S37), R37 (= R38), D38 (= D39), K41 (= K42), D60 (= D61), V61 (= V62), A80 (= A81), A81 (= A82), A82 (= A83), K84 (= K85), T99 (= T100), L122 (= L123), K138 (= K139), Y164 (= Y165)
3vvbA Crystal structure of capsular polysaccharide synthesizing enzyme cape from staphylococcus aureus in apo form (see paper)
34% identity, 98% coverage: 2:326/333 of query aligns to 1:258/270 of 3vvbA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), T12 (= T13), G13 (= G14), S14 (= S15), F15 (= F16), S35 (= S37), R36 (= R38), D37 (= D39), K40 (= K42), D59 (= D61), V60 (= V62), A80 (= A82), A81 (= A83), K83 (= K85), L121 (= L123), T123 (= T125), K137 (= K139), Y163 (= Y165), G164 (= G166)
4tqgA Crystal structure of megavirus udp-glcnac 4,6-dehydratase, 5-epimerase mg534 (see paper)
31% identity, 87% coverage: 2:292/333 of query aligns to 3:276/297 of 4tqgA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G11), S14 (≠ T13), G15 (= G14), S16 (= S15), L17 (≠ F16), R36 (= R38), D37 (= D39), D59 (= D61), I60 (≠ V62), A81 (= A81), A82 (= A82), A83 (= A83), K85 (= K85), V128 (≠ L123), Y140 (= Y135), K144 (= K139), Y168 (= Y165), G169 (= G166), V171 (= V168)
5bjuA X-ray structure of the pglf dehydratase from campylobacter jejuni in complex with udp and NAD(h) (see paper)
30% identity, 81% coverage: 2:272/333 of query aligns to 22:297/340 of 5bjuA
- binding nicotinamide-adenine-dinucleotide: G31 (= G11), G34 (= G14), T35 (≠ S15), I36 (≠ F16), D56 (≠ S37), H57 (≠ R38), S82 (≠ D61), I83 (≠ V62), A104 (= A81), A105 (= A82), A106 (= A83), K108 (= K85), N123 (≠ T100), I146 (≠ L123), K162 (= K139), F184 (≠ Y165), G185 (= G166), N186 (= N167), V187 (= V168), S190 (= S171), S191 (≠ R172)
- binding uridine-5'-diphosphate: K150 (= K127), N186 (= N167), S193 (= S174), V194 (= V175), T209 (≠ P191), L210 (≠ I192), T211 (= T193), I215 (≠ M197), R217 (= R199), E279 (= E255)
5bjvA X-ray structure of the pglf udp-n-acetylglucosamine 4,6-dehydratase from campylobacterjejuni, d396n/k397a variant in complex with udp-n- acrtylglucosamine (see paper)
30% identity, 80% coverage: 5:272/333 of query aligns to 25:297/339 of 5bjvA
- binding nicotinamide-adenine-dinucleotide: G31 (= G11), G34 (= G14), T35 (≠ S15), I36 (≠ F16), D56 (≠ S37), H57 (≠ R38), S82 (≠ D61), I83 (≠ V62), A104 (= A81), A105 (= A82), A106 (= A83), K108 (= K85), N123 (≠ T100), I146 (≠ L123), K162 (= K139), F184 (≠ Y165), G185 (= G166), N186 (= N167), V187 (= V168), S190 (= S171), S191 (≠ R172)
- binding uridine-diphosphate-n-acetylglucosamine: K108 (= K85), H109 (≠ Q86), T148 (= T125), G185 (= G166), N186 (= N167), S193 (= S174), V194 (= V175), T209 (≠ P191), L210 (≠ I192), T211 (= T193), I215 (≠ M197), R217 (= R199), R276 (= R252), E279 (= E255)
1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
28% identity, 65% coverage: 7:224/333 of query aligns to 3:240/322 of 1r66A
- active site: T127 (= T125), D128 (= D126), E129 (≠ K127), Y151 (= Y135), K155 (= K139)
- binding nicotinamide-adenine-dinucleotide: G10 (= G14), F11 (≠ S15), I12 (≠ F16), D37 (= D39), S38 (≠ E40), L39 (= L41), T40 (≠ K42), G43 (≠ M46), D63 (= D61), I64 (≠ V62), F83 (≠ A81), A84 (= A82), A85 (= A83), S87 (≠ K85), T102 (= T100), V125 (≠ L123), S126 (= S124), Y151 (= Y135), K155 (= K139), N181 (≠ R164)
- binding thymidine-5'-diphosphate: H88 (≠ Q86), E129 (≠ K127), N180 (≠ V163), K190 (≠ S174), L191 (≠ V175), P206 (≠ L190), Y208 (≠ I192), R215 (= R199)
Sites not aligning to the query:
1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
27% identity, 65% coverage: 7:224/333 of query aligns to 3:240/322 of 1r6dA
- active site: T127 (= T125), N128 (≠ D126), Q129 (≠ K127), Y151 (= Y135), K155 (= K139)
- binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (≠ K85), H88 (≠ Q86), T127 (= T125), N128 (≠ D126), Q129 (≠ K127), Y151 (= Y135), N180 (≠ V163), K190 (≠ S174), L191 (≠ V175), P206 (≠ L190), Y208 (≠ I192), R215 (= R199)
- binding nicotinamide-adenine-dinucleotide: G10 (= G14), F11 (≠ S15), I12 (≠ F16), D37 (= D39), S38 (≠ E40), L39 (= L41), T40 (≠ K42), A42 (≠ E45), G43 (≠ M46), D63 (= D61), I64 (≠ V62), F83 (≠ A81), A84 (= A82), A85 (= A83), S87 (≠ K85), T102 (= T100), V125 (≠ L123), S126 (= S124), Y151 (= Y135), K155 (= K139), N181 (≠ R164)
Sites not aligning to the query:
3pvzA Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
25% identity, 74% coverage: 8:253/333 of query aligns to 34:280/372 of 3pvzA
- binding nicotinamide-adenine-dinucleotide: G40 (= G14), S41 (= S15), I42 (≠ F16), D62 (≠ S37), I63 (≠ R38), D92 (vs. gap), I93 (vs. gap), L114 (≠ A81), S115 (≠ A82), A116 (= A83), K118 (= K85), V158 (≠ L123), D161 (= D126), K174 (= K139), V198 (= V168), S201 (= S171)
6jkhA The NAD+-bound form of human nsdhl (see paper)
28% identity, 44% coverage: 5:151/333 of query aligns to 3:139/212 of 6jkhA
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), F13 (≠ S15), L14 (≠ F16), D33 (≠ E45), I34 (≠ M46), G48 (= G60), L50 (≠ V62), A70 (= A82), S71 (≠ A83), Y123 (= Y135), K127 (= K139)
3pvzB Udp-n-acetylglucosamine 4,6-dehydratase from vibrio fischeri
28% identity, 51% coverage: 8:176/333 of query aligns to 32:204/300 of 3pvzB
- binding nicotinamide-adenine-dinucleotide: G38 (= G14), S39 (= S15), I40 (≠ F16), D60 (≠ S37), I61 (≠ R38), L89 (vs. gap), D90 (vs. gap), I91 (vs. gap), L112 (≠ A81), S113 (≠ A82), A114 (= A83), K116 (= K85), D159 (= D126), K172 (= K139), N195 (= N167), V196 (= V168), S199 (= S171)
Query Sequence
>Pf6N2E2_2611 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2611
MFNGKSIFISGGTGSFGRNFIRRLLEQYQPKRVVVFSRDELKQYEMQQTFNAPCMRYFLG
DVRDADRLRQAMRGIDYVVHAAALKQVPAAEYNPTECIRTNVNGAENIIAAAIDNGVKKV
VALSTDKAASPINLYGATKLLSDKLFVAANNIAGEQQTRFAVVRYGNVAGSRGSVVPFFS
KLIADGATQLPITDERMTRFWITLDHGVQFVLDSFARMHGGEVFVPKIPSIRIVDLASGM
AGHLPHKNVGIRPGEKLHELMVPLDDARMTIEFADHYTIQPSIRFTSVDVDFGVDNLGER
GKPVAEDFEYRSDTNPHFLSVEQIADLHARLSA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory