SitesBLAST

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
28% identity, 48% coverage: 80:259/372 of query aligns to 6:181/187 of O05306

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O05306

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M54 (vs. gap) mutation to A: Large decrease in catalytic activity.
K74 (≠ I145) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (≠ Q151) mutation to A: Large decrease in catalytic activity.
R98 (= R172) mutation to A: Loss of catalytic activity.
T118 (= T192) mutation to A: Loss of catalytic activity.
DE 120:121 (= DE 194:195) mutation to AA: Loss of catalytic activity.

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
31% identity, 48% coverage: 85:261/372 of query aligns to 36:216/242 of Q5ZC82

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Q5ZC82

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G73 (= G122) mutation to D: In LOG-4; decreased activity.

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
31% identity, 42% coverage: 85:241/372 of query aligns to 4:157/195 of P48636

query
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P48636

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E74 (≠ N154) binding
M89 (≠ F169) mutation to A: Almost complete loss of activity.
R92 (= R172) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 172:173) binding
109:115 (vs. 189:195, 71% identical) binding
T112 (= T192) mutation to A: Almost complete loss of activity.
E114 (≠ D194) mutation to A: Almost complete loss of activity.
E115 (= E195) mutation to A: Almost complete loss of activity.
E118 (= E198) mutation to A: Retains 8% of wild-type activity.

Query Sequence

>Pf6N2E2_2991 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2991
MPYKPNDLLSRHFENHGHDLTRKVEEQLNLVSPNSPNLPIYRDMILTVLRMAQEDHNRWN
AKITLQALRELEHAFRTLEQFKGRRKVTVFGSARTPIEHPLYGLARELGAALARSDMMVI
TGAGGGIMAAAHEGAGRDHSLGFNITLPFEQHANPTVEGTPNLLPFHFFFTRKLFFVKEA
DALVLCPGGFGTLDEALEVLTLVQTGKSPLVPVVLLDVPGGKFWQGALNFIREQLEENRY
ILPTDMKLMRLVYNAEEAVEEINQFYRNFHSSRWLKHQFVIRMNHKLNEQALQQMQGEFA
DLCLNDCFHQHDYSGEEHDEAQFSHLTRLAFAFNARDHGRLRELVDYINLPQNWAQPASK
PHPLTWEPIKVT

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory