SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_3044 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3044 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P81600 Alcohol dehydrogenase class-3 chain H; Alcohol dehydrogenase class-III chain H; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
66% identity, 100% coverage: 1:370/370 of query aligns to 6:375/375 of P81600

query
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P81600

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Sites not aligning to the query:

1 modified: N-acetylalanine

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
65% identity, 100% coverage: 2:370/370 of query aligns to 7:375/375 of P81601

query
sites
P81601

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Sites not aligning to the query:

1 modified: N-acetylalanine

P80467 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
66% identity, 100% coverage: 1:370/370 of query aligns to 4:373/373 of P80467

query
sites
P80467

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Sites not aligning to the query:

1 modified: N-acetylalanine

P11766 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class chi chain; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Homo sapiens (Human) (see 3 papers)
66% identity, 99% coverage: 1:368/370 of query aligns to 5:372/374 of P11766

query
sites
P11766

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D

K

P

E
x
L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

P

K

A

D

K

K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

Q

K

L

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V
|
V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R

R

T

T

V

V

I

V

C45 (= C41) binding
H67 (= H63) binding
C97 (= C93) binding
C100 (= C96) binding
C103 (= C99) binding
C111 (= C107) binding
R115 (= R111) Important for FDH activity and activation by fatty acids; mutation R->A,D: Loss of FDH activity and loss of activation by fatty acids.
L163 (≠ E159) to S: in dbSNP:rs28730623
C174 (= C170) binding
V309 (= V305) to I: in dbSNP:rs28730628

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2fzeA Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with adp-ribose (see paper)
66% identity, 99% coverage: 1:368/370 of query aligns to 4:371/373 of 2fzeA

query
sites
2fzeA

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

L

E

S

I

I

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

S

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A
x
K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C
|
C

G

G

Q

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

A

K

I

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K
|
K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G
|
G

L
|
L

G
|
G

I
x
V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I
x
V

D
|
D

I
|
I

N

N

P

K

A

D

K
|
K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G

G

N

N

V

V

Q

K

L
x
V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C41), H45 (= H42), T46 (= T43), Y49 (= Y46), H66 (= H63), E67 (= E64), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), R114 (= R111), C173 (= C170), T177 (= T174), R368 (= R365)
binding adenosine-5-diphosphoribose: H45 (= H42), G198 (= G195), L199 (= L196), G200 (= G197), G201 (= G198), V202 (≠ I199), V221 (≠ I218), D222 (= D219), I223 (= I220), K227 (= K224), C267 (= C264), I268 (= I265), V273 (≠ L270), V291 (≠ I288), G292 (= G289), V293 (= V290), R368 (= R365)
binding phosphate ion: K83 (≠ A80), K158 (= K155)
binding zinc ion: H66 (= H63), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), C173 (= C170)

1mc5A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with s-(hydroxymethyl)glutathione and nadh (see paper)
66% identity, 99% coverage: 1:368/370 of query aligns to 4:371/373 of 1mc5A

query
sites
1mc5A

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

L

E

S

I

I

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

L

S

S

G

G

A

A

D
|
D

P
|
P

E
|
E

G

G

I

C

F

F

P

P

S

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P

P

E

Q

C
|
C

G

G

Q

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

A

K

I

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G
|
G

L
|
L

G
|
G

I
x
V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I
x
V

D
|
D

I
|
I

N

N

P

K

A

D

K
|
K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G
|
G

N

N

V

V

Q

K

L

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S
x
T

A
|
A

F
|
F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C41), H45 (= H42), T46 (= T43), Y49 (= Y46), H66 (= H63), E67 (= E64), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), R114 (= R111), C173 (= C170), T177 (= T174), R368 (= R365)
binding 2-amino-4-[1-carboxymethyl-carbamoyl)-2-hydroxymethylsulfanyl-ethylcarbamoyl]-butyric acid: T46 (= T43), D55 (= D52), P56 (= P53), E57 (= E54), H66 (= H63), Y92 (= Y89), I93 (≠ T90), R114 (= R111), C173 (= C170), V293 (= V290), A317 (= A314)
binding nicotinamide-adenine-dinucleotide: C44 (= C41), H45 (= H42), T46 (= T43), Y92 (= Y89), C173 (= C170), T177 (= T174), G198 (= G195), L199 (= L196), G200 (= G197), G201 (= G198), V202 (≠ I199), V221 (≠ I218), D222 (= D219), I223 (= I220), K227 (= K224), C267 (= C264), I268 (= I265), G269 (= G266), V291 (≠ I288), G292 (= G289), V293 (= V290), T316 (≠ S313), A317 (= A314), F318 (= F315), R368 (= R365)
binding zinc ion: C44 (= C41), H66 (= H63), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), C173 (= C170)

1ma0A Ternary complex of human glutathione-dependent formaldehyde dehydrogenase with NAD+ and dodecanoic acid (see paper)
66% identity, 99% coverage: 1:368/370 of query aligns to 4:371/373 of 1ma0A

query
sites
1ma0A

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

L

E

S

I

I

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

S

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T

I

P

P

E

Q

C
|
C

G

G

Q

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C
|
C

Q

Q

A

K

I

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G
|
G

L
|
L

G
|
G

I
x
V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I
x
V

D
|
D

I
|
I

N

N

P

K

A

D

K
|
K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G
|
G

N

N

V

V

Q

K

L

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S
x
T

A
|
A

F
|
F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C41), H45 (= H42), T46 (= T43), Y49 (= Y46), H66 (= H63), E67 (= E64), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), R114 (= R111), C173 (= C170), T177 (= T174), R368 (= R365)
binding nicotinamide-adenine-dinucleotide: H45 (= H42), Y92 (= Y89), T177 (= T174), G198 (= G195), L199 (= L196), G200 (= G197), G201 (= G198), V202 (≠ I199), V221 (≠ I218), D222 (= D219), I223 (= I220), K227 (= K224), C267 (= C264), I268 (= I265), G269 (= G266), V291 (≠ I288), G292 (= G289), V293 (= V290), T316 (≠ S313), A317 (= A314), F318 (= F315), R368 (= R365)
binding zinc ion: C44 (= C41), H66 (= H63), E67 (= E64), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), C173 (= C170)

1m6wA Binary complex of human glutathione-dependent formaldehyde dehydrogenase and 12-hydroxydodecanoic acid (see paper)
66% identity, 99% coverage: 1:368/370 of query aligns to 4:371/373 of 1m6wA

query
sites
1m6wA

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

L

E

S

I

I

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

S

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P

P

E

Q

C
|
C

G

G

Q

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L
|
L

C
|
C

Q
|
Q

A

K

I

I

R
|
R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M
|
M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G

G

L

L

G

G

I

V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

P

K

A

D

K

K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

Q

K

L

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G
|
G

V
|
V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A
|
A

F

F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C44 (= C41), H45 (= H42), T46 (= T43), Y49 (= Y46), H66 (= H63), E67 (= E64), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), R114 (= R111), C173 (= C170), T177 (= T174), R368 (= R365)
binding 12-hydroxydodecanoic acid: T46 (= T43), H66 (= H63), Y92 (= Y89), I93 (≠ T90), L109 (= L106), Q111 (= Q108), R114 (= R111), M140 (= M137), C173 (= C170), G292 (= G289), V293 (= V290), A317 (= A314)
binding zinc ion: C44 (= C41), H66 (= H63), C96 (= C93), C99 (= C96), C102 (= C99), C110 (= C107), C173 (= C170)

3qj5A S-nitrosoglutathione reductase (gsnor) in complex with n6022 (see paper)
66% identity, 99% coverage: 1:368/370 of query aligns to 3:370/372 of 3qj5A

query
sites
3qj5A

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

L

E

S

I

I

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

C

F

F

P

P

S

V

I

I

L

L

G

G

H
|
H

E
|
E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y
|
Y

T
x
I

P

P

E

Q

C
|
C

G

G

Q

E

C
|
C

K

K

F

F

C
|
C

K

L

S

N

G

P

K

K

T

T

N

N

L
|
L

C
|
C

Q
|
Q

A

K

I

I

R
|
R

A

V

T

T

Q
|
Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M
|
M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C
|
C

G

G

V

I

T

S

T
|
T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

L

K

E

P

P

G

G

D

S

T

V

V

C

A

A

I

V

F

F

G
|
G

L
|
L

G
|
G

I
x
V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I
x
V

D
|
D

I
|
I

N

N

P

K

A

D

K
|
K

F

F

E

A

I

R

A

A

K

K

Q

E

L

F

G

G

A

A

T

T

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C
|
C

I
|
I

G
|
G

N

N

V

V

Q

K

L
x
V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K
|
K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

I
x
V

G
|
G

V
|
V

A
|
A

G
x
A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T
|
T

G

G

R

R

V

T

W

W

R

K

G

G

S
x
T

A
|
A

F
|
F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

N

M

L

G

S

L

F

E

D

D

E

I

I

N

N

K

K

A

A

F

F

D

E

L

L

M

M

H

H

E

S

G

G

K

K

S

S

I

I

R
|
R

T

T

V

V

I

V

active site: C43 (= C41), H44 (= H42), T45 (= T43), Y48 (= Y46), H65 (= H63), E66 (= E64), C95 (= C93), C98 (= C96), C101 (= C99), C109 (= C107), R113 (= R111), C172 (= C170), T176 (= T174), R367 (= R365)
binding 3-{1-(4-carbamoyl-2-methylphenyl)-5-[4-(1H-imidazol-1-yl)phenyl]-1H-pyrrol-2-yl}propanoic acid: C43 (= C41), T45 (= T43), H65 (= H63), Y91 (= Y89), I92 (≠ T90), L108 (= L106), Q110 (= Q108), R113 (= R111), Q116 (= Q114), M139 (= M137), C172 (= C170), K282 (= K280), V292 (= V290), T308 (= T306), A316 (= A314)
binding pentane-1,5-diamine: H44 (= H42), Y48 (= Y46), V292 (= V290), A293 (= A291), A294 (≠ G292)
binding nicotinamide-adenine-dinucleotide: H44 (= H42), Y91 (= Y89), C172 (= C170), T176 (= T174), G197 (= G195), L198 (= L196), G199 (= G197), G200 (= G198), V201 (≠ I199), V220 (≠ I218), D221 (= D219), I222 (= I220), K226 (= K224), C266 (= C264), I267 (= I265), G268 (= G266), V272 (≠ L270), V290 (≠ I288), G291 (= G289), V292 (= V290), T315 (≠ S313), A316 (= A314), F317 (= F315), R367 (= R365)
binding zinc ion: C43 (= C41), H65 (= H63), C95 (= C93), C98 (= C96), C101 (= C99), C109 (= C107), C172 (= C170)

P19854 Alcohol dehydrogenase class-3; Alcohol dehydrogenase 5; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Equus caballus (Horse) (see 2 papers)
66% identity, 100% coverage: 1:370/370 of query aligns to 5:374/374 of P19854

query
sites
P19854

M

V

I

I

K

K

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

G

K

K

P

P

L

V

E

S

I

I

V

E

E

E

V

V

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

I

R

K

V

I

A

A

S

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

Y

Y

T

T

L

L

S

S

G

G

A

A

D

D

P

P

E

E

G

G

I

S

F

F

P

P

S

V

I

I

L

L

G

G

H

H

E

E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

L

A

K

V

A

G

G

D

D

H

T

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

G

G

Q

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

Q

T

T

N

N

L

L

C

C

Q

Q

A

K

I

I

R

R

A

T

T

T

Q

Q

G

G

K

K

G

G

L

L

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

F

S

T

Y

C

K

K

G

G

E

K

T

T

I

I

F

L

H

H

Y

Y

M

M

G

G

T

T

S

S

T

T

F

F

S

S

E

E

Y

Y

T

T

V

V

L

V

P

A

E

D

I

I

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

L

A

A

P

P

L

L

E

D

K

K

V

V

C

C

L

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

I

Y

G

G

A

A

V

A

L

V

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

T

V

C

A

A

I

I

F

F

G

G

L

L

G

G

I

V

G

G

L

L

S

A

A

V

V

I

I

M

G

G

A

C

V

K

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

G

I

V

D

D

I

I

N

N

P

K

A

D

K

K

F

F

E

A

I

K

A

A

K

K

Q

E

L

F

G

G

A

A

T

S

D

E

C

C

V

I

N

N

P

P

K

Q

D

D

F

F

D

S

R

K

P

P

I

I

Q

Q

E

E

V

V

I

L

V

I

D

E

M

M

T

T

D

D

G

G

V

V

D

D

F

Y

S

S

F

F

E

E

C

C

I

I

G

G

N

N

V

V

Q

K

L

V

M

M

R

R

A

A

L

L

E

E

C

A

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

V

G

G

V

V

A

A

G

A

A

S

G

G

Q

E

E

E

I

I

A

A

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

T

A

A

F

F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

I

P

P

S

K

Y

L

V

V

D

S

M

E

A

Y

E

M

K

S

G

K

E

K

I

I

P

K

L

V

D

D

T

E

F

F

I

V

T

T

H

H

T

S

M

L

G

S

L

F

E

D

D

Q

I

I

N

N

K

E

A

A

F

F

D

E

L

L

M

M

H

H

E

A

G

G

K

K

S

S

I

I

R

R

T

T

V

V

I

V

H

K

F

L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

P80360 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Myxine glutinosa (Atlantic hagfish) (see 2 papers)
63% identity, 100% coverage: 1:370/370 of query aligns to 7:376/376 of P80360

query
sites
P80360

M

V

I

I

K

H

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

A

K

K

P

P

L

L

E

S

I

L

V

E

E

E

V

I

D

E

V

V

A

A

M

P

P

P

K

K

A

A

G

H

E

E

V

V

L

R

L

M

R

K

V

V

V

L

A

A

S

T

G

A

V

V

C

C

H

H

T

T

D

D

A

A

Y

Y

T

T

L

L

S

S

G

G

A

V

D

D

P

P

E

E

G

G

I

S

F

F

P

P

S

V

I

V

L

L

G

G

H

H

E

E

G

G

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

K

V

F

A

K

V

P

G

G

D

D

H

S

V

V

I

I

P

P

L

L

Y

Y

T

I

P

P

E

Q

C

C

G

G

Q

E

C

C

K

K

F

F

C

C

K

L

S

N

G

P

K

K

T

T

N

N

L

L

C

C

Q

Q

A

K

I

I

R

R

A

V

T

T

Q

Q

G

G

K

K

G

G

L

M

M

M

P

P

D

D

G

G

T

T

S

S

R

R

F

L

S

T

Y

C

K

R

G

G

E

K

T

S

I

L

F

Y

H

H

Y

F

M

M

G

G

T

A

S

S

T

T

F

F

S

S

E

E

Y

Y

T

A

V

V

L

V

P

A

E

D

I

I

S

S

V

L

A

C

K

R

I

V

P

A

K

P

E

E

A

A

P

P

L

P

E

D

K

R

V

V

C

C

L

L

G

G

C

C

G

G

V

V

T

S

T

T

G

G

I

Y

G

G

A

A

V

P

L

L

N

N

T

T

A

A

K

K

V

V

K

E

P

P

G

G

D

S

T

T

V

C

A

A

I

I

F

F

G

G

L

L

G

G

G

A

I

V

G

G

L

L

S

A

A

A

V

I

I

M

G

G

A

C

V

R

K

V

A

A

K

G

A

A

S

S

R

R

I

I

A

A

I

I

D

D

I

R

N

N

P

P

A

D

K

K

F

F

E

E

I

K

A

A

K

R

Q

I

L

F

G

G

A

A

T

T

D

D

C

C

V

V

N

V

P

P

K

D

D

A

F

S

D

D

R

K

P

P

I

I

Q

S

E

Q

V

V

I

L

V

G

D

E

M

M

T

T

D

D

G

G

V

L

D

D

F

Y

S

T

F

F

E

E

C

C

I

V

G

G

N

N

V

V

Q

G

L

I

M

M

R

R

A

A

L

L

E

E

C

S

C

C

H

H

K

K

G

G

W

W

G

G

E

V

S

S

V

V

I

I

I

L

G

G

V

V

A

A

G

G

A

G

G

G

Q

Q

E

E

I

I

A

S

T

T

R

R

P

P

F

F

Q

Q

L

L

V

V

T

T

G

G

R

R

V

T

W

W

R

K

G

G

S

A

A

A

F

F

G

G

V

W

R

K

G

S

R

V

S

E

E

S

L

V

P

P

S

K

Y

L

V

V

D

D

M

D

A

Y

E

M

K

A

G

G

E

K

I

I

P

M

L

V

D

D

T

E

F

F

I

V

T

S

H

H

T

S

M

L

G

P

L

F

E

D

D

S

I

I

N

N

K

E

A

A

F

F

D

D

L

L

M

M

H

H

E

A

G

G

K

K

S

S

I

I

R

R

T

T

V

V

I

L

H

Q

F

L

Sites not aligning to the query:

1 modified: N-acetylserine

4dl9A Crystal structure of s-nitrosoglutathione reductase from tomato (solanum lycopersicum) in complex with NAD+ (see paper)
64% identity, 99% coverage: 1:368/370 of query aligns to 7:375/379 of 4dl9A

query
sites
4dl9A

M

V

I

I

K

T

S

C

R

K

A

A

V

V

A

A

F

W

E

E

A

P

K

N

K

K

P

P

L

L

E

V

I

I

V

E

E

D

V

V

D

Q

V

V

A

A

M

P

P

P

K

Q

A

A

G

G

E

E

V

V

L

R

L

V

R

K

V

V

V

L

A

Y

S

T

G

A

V

L

C
|
C

H
|
H

T
|
T

D

D

A

A

Y
|
Y

T

T

L

W

S

S

G

G

A

K

D

D

P

P

E

E

G

G

I

L

F

F

P

P

S

C

I

V

L

L

G

G

H
|
H

E
|
E

G

A

A

G

I

I

V

V

E

E

A

S

V

V

G

G

E

E

G

G

V

V

T

T

S

E

V

V

A

Q

V

P

G

G

D

D

H

H

V

V

I

I

P

P

L

C

Y
|
Y

T

Q

P

A

E

E

C
|
C

G

R

Q

E

C
|
C

K

K

F

F

C
|
C

K

K

S

S

G

G

K

K

T

T

N

N

L

L

C
|
C

Q

G

A

K

I

V

R
|
R

A

A

T

A

Q

T

G

G

K

V

G

G

-

V

L

M

M

M

P

N

D

D

G

R

T

K

S

S

R

R

F

F

S

S

Y

I

K

N

G

G

E

K

T

P

I

I

F

Y

H

H

Y

F

M

M

G

G

T

T

S

S

T

T

F

F

S

S

E

Q

Y

Y

T

T

V

V

L

V

P

H

E

D

I

V

S

S

V

V

A

A

K

K

I

I

P

D

K

P

E

V

A

A

P

P

L

L

E

E

K

K