SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_3589 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3589 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
79% identity, 99% coverage: 1:194/195 of query aligns to 1:194/195 of P48636

query
sites
P48636

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E74 (= E74) binding
M89 (= M89) mutation to A: Almost complete loss of activity.
R92 (= R92) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 92:93) binding
109:115 (vs. 109:115, 100% identical) binding
T112 (= T112) mutation to A: Almost complete loss of activity.
E114 (= E114) mutation to A: Almost complete loss of activity.
E115 (= E115) mutation to A: Almost complete loss of activity.
E118 (= E118) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
80% identity, 93% coverage: 2:183/195 of query aligns to 1:182/182 of 5zbkA

query
sites
5zbkA

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binding adenosine monophosphate: A11 (= A12), E73 (= E74), M88 (= M89), R91 (= R92), K92 (= K93), G108 (= G109), G110 (= G111), T111 (= T112)

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
51% identity, 93% coverage: 2:183/195 of query aligns to 1:181/181 of 5zblD

query
sites
5zblD

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binding adenosine monophosphate: S11 (≠ A12), M47 (= M48), R90 (= R92), K91 (= K93), G107 (= G109), G109 (= G111), T110 (= T112), E112 (= E114), E113 (= E115)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
47% identity, 97% coverage: 6:194/195 of query aligns to 38:228/242 of Q5ZC82

query
sites
Q5ZC82

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G73 (= G41) mutation to D: In LOG-4; decreased activity.

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
39% identity, 97% coverage: 4:192/195 of query aligns to 3:217/217 of 5ajuA

query
sites
5ajuA

A

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binding 5-O-phosphono-beta-D-ribofuranose: S11 (≠ A12), R108 (= R92), G131 (= G109), Y132 (≠ L110), G133 (= G111), T134 (= T112), E137 (= E115)

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
42% identity, 89% coverage: 5:177/195 of query aligns to 8:179/183 of 7w2iA

query
sites
7w2iA

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binding magnesium ion: D120 (≠ E118), D124 (≠ W122)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
42% identity, 92% coverage: 5:184/195 of query aligns to 12:187/187 of O05306

query
sites
O05306

S

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M54 (= M48) mutation to A: Large decrease in catalytic activity.
K74 (≠ Q68) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E74) mutation to A: Large decrease in catalytic activity.
R98 (= R92) mutation to A: Loss of catalytic activity.
T118 (= T112) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 114:115) mutation to AA: Loss of catalytic activity.

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
40% identity, 89% coverage: 5:177/195 of query aligns to 3:174/177 of 3sbxA

query
sites
3sbxA

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binding adenosine monophosphate: E71 (= E74), M86 (= M89), R89 (= R92), K90 (= K93), G106 (= G109), G108 (= G111), T109 (= T112), D111 (≠ E114), E112 (= E115)

Query Sequence

>Pf6N2E2_3589 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3589
MSIASVCVFCGASTGTDPAYREAAQALGRALAERKLTLVYGGGAVGLMGIVADAALAAGG
EVIGIIPQSLKDKEIGHSGLTRLEVVDGMHARKARMAELSDAFIALPGGLGTLEELFEVW
TWGQLGYHGKPLGLLEVNGFYSKLIGFLDHIVDEGFVRAPHRDMLQVSESAQSLLDALDE
WQPSVQPKWAEQKPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory