SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_368 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_368 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
61% identity, 99% coverage: 5:483/485 of query aligns to 2:481/482 of P25526

query
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P25526

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WN 156:157 (= WN 159:160) binding
KPAS 180:183 (≠ KPSD 183:186) binding
GS 233:234 (= GS 236:237) binding
L256 (= L259) binding
E386 (= E388) binding

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
61% identity, 99% coverage: 5:483/485 of query aligns to 1:480/481 of 3jz4A

query
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3jz4A

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active site: N156 (= N160), K179 (= K183), E254 (= E258), C288 (= C292), E385 (= E388), E462 (= E465)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I152 (= I156), P154 (= P158), W155 (= W159), N156 (= N160), K179 (= K183), A181 (≠ S185), S182 (≠ D186), S211 (≠ M215), A212 (≠ P216), G213 (≠ A217), G216 (= G220), F230 (= F234), T231 (= T235), G232 (= G236), S233 (= S237), I236 (≠ V240), E254 (= E258), L255 (= L259), C288 (= C292), K338 (= K342), E385 (= E388), F387 (= F390)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
54% identity, 98% coverage: 10:485/485 of query aligns to 57:535/535 of P51649

query
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P51649

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V

A

E

E

E

A

V

M

G

K

K

K

-

N

L

L

K

R

V

V

G

G

N
|
N

G

G

L

F

E

E

A

E

G

G

V

T

M

T

I

Q

G

G

P
|
P

L

L

I

I

N

N

L

E

A

K

A

A

V

V

N

E

K

K

V

V

A

E

R

K

H

Q

I

V

D

N

D

D

A

A

L

V

S

S

Q

K

G

G

A

A

R

T

L
x
V

L

V

C

T

G
|
G

G

G

V

K

-

R

P

H

E

Q

G

L

D

G

S

K

Q

N

F

F

V

F

Q

E

P

P

T

T

V

L

L

L

G

C

E

N

A

V

H

T

A

Q

G

D

M

M

L

L

L

C

A

T

N

H

E

E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

M

I

R

K

F

F

T

D

T

T

E

E

Q

E

A

A

L

I

A

A

L

I

A

A

N

N

A

A

T

A

P

D

Y

V

G

G

L

L

G

A

A

G

Y

Y

Y

F

F

Y

T

S

Q

Q

D

D

L

P

R

A

R

Q

S

I

W

W

R

R

F

V

G

A

E

E

A

Q

L

L

E

E

F

V

G

G

M

M

V

V

G

G

L

V

N

N

T

E

G

G

I

L

I

I

S
|
S

M

S

E

V

V

E

A

C

P

P

F

F

G

G

I

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E

E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

L

L

E

E

V

L

K

K

A

Y

F

V

H

C

I

Y

G
|
G

G

G

L

L

C93 (≠ M48) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G131) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P135) to Y: 83% of activity; dbSNP:rs2760118
P182 (= P137) to L: 48% of activity; dbSNP:rs3765310
R213 (= R168) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C178) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPSD 183:186) binding
T233 (= T188) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A192) to S: 65% of activity; dbSNP:rs62621664
N255 (= N210) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (= G220) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTAVG 236:241) binding
R334 (= R286) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (= N287) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C292) modified: Disulfide link with 342, In inhibited form
C342 (= C294) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (= N323) natural variant: N -> S
P382 (= P333) to L: in SSADHD; 2% of activity
V406 (≠ L357) to I: in dbSNP:rs143741652
G409 (= G360) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (= S448) binding ; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G483) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
54% identity, 98% coverage: 10:485/485 of query aligns to 7:485/485 of 2w8rA

query
sites
2w8rA

S

A

L

L

A

R

E

T

L

D

A

S

Y

F

V

V

D

G

G

G

Q

R

W

W

I

L

G

P

A

A

D

-

N

-

A

A

T

T

L

F

D

P

V

V

I

Q

D

D

P

P

A

A

T

S

G

G

H

A

S

A

L

L

A

G

R

M

V

V

P

A

A

D

M

C

Q

G

G

V

V

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

E

Y

R

E

A

A

W

F

P

C

A

R

W

W

R

R

A

E

R

V

P

S

A

A

A

K

E

E

R

R

A

S

L

L

E

R

R

K

W

W

Y

Y

Q

N

A

L

M

M

M

I

D

Q

N

N

L

K

D

D

L

L

A

A

L

R

I

I

M

I

T

T

C

A

E

E

Q

S

G

G

K

K

P

P

L

L

S

K

E

E

A

A

K

H

G

G

E

E

I

I

R

L

Y

Y

G

S

A

A

G

F

F

F

A

L

K

E

W

W

F

F

A

S

E

E

A

A

R

R

V

V

Y

Y

G

G

E

D

T

I

I

I

P

H

A

T

P

P

S

A

G

K

D

D

R

R

L

A

L

L

T

V

L

L

K

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I
|
I

T
|
T

P
|
P

W
|
W

N
|
N

F

F

P

P

N

S

A

A

M

M

I

I

T

T

R

R

K

K

C

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

P

T

V

V

I

V

V

V

K
|
K

P
|
P

S
x
A

D
x
E

L

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

V

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

N

N

V

V

V

I

T

P

G

C

M

S

P

R

A

K

G

N

-
x
A

-
x
K

-

E

I

V

G

G

E

E

E

A

L

I

T

C

G

T

N

D

P

P

T

L

V

V

R

S

K

K

I

I

S

S

F
|
F

T

T

G

G

S
|
S

T

T

A

T

V
x
T

G

G

R

K

L

I

L

L

M

L

R

H

Q

H

S

A

A

A

E

N

H

S

I

V

K

K

R

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

E

D

Q

Q

A

A

V

V

A

A

G

G

I

A

M

M

L

A

S

S

K

K

F

F

R

R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V

V

C

C

A

S

N

N

R

Q

I

F

L

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

E

D

R

A

F

F

A

V

A

K

R

A

L

F

V

A

E

E

E

A

V

M

G

K

K

K

-

N

L

L

K

R

V

V

G

G

N

N

G

G

L

F

E

E

A

E

G

G

V

T

M

T

I

Q

G

G

P

P

L

L

I

I

N

N

L

E

A

K

A

A

V

V

N

E

K

K

V

V

A

E

R

K

H

Q

I

V

D

N

D

D

A

A

L

V

S

S

Q

K

G

G

A

A

R

T

L

V

C

T

G

G

V

K

-

R

P

H

E

Q

G

L

D

G

S

K

Q

N

F

F

V

F

Q

E

P

P

T

T

V

L

L

L

G

C

E

N

A

V

H

T

A

Q

G

D

M

M

L

L

L

C

A

T

N

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

M

I

R

K

F

F

T

D

T

T

E

E

Q

E

A

A

L

I

A

A

L

I

A

A

N

N

A

A

T

A

P

D

Y

V

G

G

L

L

G

A

A

G

Y

Y

Y

F

F

Y

T

S

Q

Q

D

D

L

P

R

A

R

Q

S

I

W

W

R

R

F

V

G

A

E

E

A

Q

L

L

E

E

F

V

G

G

M

M

V

V

G

G

L

V

N

N

T

E

G

G

I

L

I

I

S

S

M

S

E

V

V

E

A

C

P

P

F

F

G

G

I

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

L

L

E

E

V

L

K

K

A

Y

F

V

H

C

I

Y

G

G

L

L

active site: N155 (= N160), K178 (= K183), E256 (= E258), A290 (≠ C292), E388 (= E388), E465 (= E465)
binding adenosine-5'-diphosphate: I151 (= I156), T152 (= T157), P153 (= P158), W154 (= W159), K178 (= K183), P179 (= P184), A180 (≠ S185), E181 (≠ D186), A214 (vs. gap), K215 (vs. gap), F232 (= F234), S235 (= S237), T238 (≠ V240)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
54% identity, 98% coverage: 10:485/485 of query aligns to 7:485/485 of 2w8qA

query
sites
2w8qA

S

A

L

L

A

R

E

T

L

D

A

S

Y

F

V

V

D

G

G

G

Q

R

W

W

I

L

G

P

A

A

D

-

N

-

A

A

T

T

L

F

D

P

V

V

I

Q

D

D

P

P

A

A

T

S

G

G

H

A

S

A

L

L

A

G

R

M

V

V

P

A

A

D

M

C

Q

G

G

V

V

R

E

E

T

A

R

R

R

A

A

A

I

V

E

R

A

A

E

Y

R

E

A

A

W

F

P

C

A

R

W

W

R

R

A

E

R

V

P

S

A

A

A

K

E

E

R

R

A

S

L

L

E

R

R

K

W

W

Y

Y

Q

N

A

L

M

M

M

I

D

Q

N

N

L

K

D

D

L

L

A

A

L

R

I

I

M

I

T

T

C

A

E

E

Q

S

G

G

K

K

P

P

L

L

S

K

E

E

A

A

K

H

G

G

E

E

I

I

R

L

Y
|
Y

G

S

A

A

G

F

F

F

A

L

K

E

W

W

F

F

A

S

E

E

A

A

R

R

V

V

Y

Y

G

G

E

D

T

I

I

I

P

H

A

T

P

P

S

A

G

K

D

D

R

R

L

A

L

L

T

V

L

L

K

K

Q

Q

P

P

V

I

G

G

V

V

C

A

A

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F
|
F

P

P

N

S

A

A

M

M

I

I

T

T

R
|
R

K

K

C

V

A

G

P

A

A

A

L

L

A

A

G

G

C

C

P

T

V

V

I

V

V

V

K
|
K

P

P

S

A

D

E

L

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

V

E

L

L

A

A

E

S

R

Q

V

A

G

G

I

I

P

P

A

S

G

G

V

V

F

Y

N

N

V

V

V

I

T

P

G

C

M

S

P

R

A

K

G

N

-

A

-

K

-

E

I

V

G

G

E

E

E

A

L

I

T

C

G

T

N

D

P

P

T

L

V

V

R

S

K

K

I

I

S

S

F

F

T

T

G

G

S

S

T

T

A

T

V

T

G

G

R

K

L

I

L

L

M

L

R

H

Q

H

S

A

A

A

E

N

H

S

I

V

K

K

R

R

L

V

S

S

L

M

E
|
E

L

L

G

G

N

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

D

N

L

V

E

D

Q

Q

A

A

V

V

A

A

G

G

I

A

M

M

L

A

S

S

K

K

F

F

R
|
R

N

N

A

T

G

G

Q

Q

T

T

C
x
A

V
|
V

C

C

A

S

N

N

R

Q

I

F

L

L

V

V

Q

Q

D

R

G

G

I

I

Y

H

E

D

R

A

F

F

A

V

A

K

R

A

L

F

V

A

E

E

E

A

V

M

G

K

K

K

-

N

L

L

K

R

V

V

G

G

N

N

G

G

L

F

E

E

A

E

G

G

V

T

M

T

I

Q

G

G

P

P

L

L

I

I

N

N

L

E

A

K

A

A

V

V

N

E

K

K

V

V

A

E

R

K

H

Q

I

V

D

N

D

D

A

A

L

V

S

S

Q

K

G

G

A

A

R

T

L

V

C

T

G

G

V

K

-

R

P

H

E

Q

G

L

D

G

S

K

Q

N

F

F

V

F

Q

E

P

P

T

T

V

L

L

L

G

C

E

N

A

V

H

T

A

Q

G

D

M

M

L

L

L

C

A

T

N

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

P

P

L

V

M

I

R

K

F

F

T

D

T

T

E

E

Q

E

A

A

L

I

A

A

L

I

A

A

N

N

A

A

T

A

P

D

Y

V

G

G

L

L

G

A

A

G

Y

Y

Y

F

F

Y

T

S

Q

Q

D

D

L

P

R

A

R

Q

S

I

W

W

R

R

F

V

G

A

E

E

A

Q

L

L

E

E

F

V

G

G

M

M

V

V

G

G

L

V

N

N

T

E

G

G

I

L

I

I

S
|
S

M

S

E

V

V

E

A

C

P

P

F
|
F

G

G

I

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

Y

Y

G

G

L

I

D

D

E

E

Y

Y

L

L

E

E

V

L

K

K

A

Y

F

V

H

C

I

Y

G

G

L

L

active site: N155 (= N160), K178 (= K183), E256 (= E258), A290 (≠ C292), E388 (= E388), E465 (= E465)
binding succinic acid: Y109 (= Y114), F156 (= F161), R163 (= R168), E256 (= E258), R284 (= R286), A290 (≠ C292), V291 (= V293), S448 (= S448), F454 (= F454)

2opxA Crystal structure of lactaldehyde dehydrogenase from escherichia coli
40% identity, 96% coverage: 17:482/485 of query aligns to 8:475/477 of 2opxA

query
sites
2opxA

Y

Y

V

I

D

D

G

G

Q

Q

W

F

I

V

G

T

A

W

D

R

N

G

A

D

A

A

T

W

L

I

D

D

V

V

I

V

D

N

P

P

A

A

T

T

G

E

H

A

S

V

L

I

A

S

R

R

V

I

P

P

A

D

M

G

Q

Q

G

A

V

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

R

L

P

P

A

A

A

I

E

E

R

R

A

A

A

S

L

W

L

L

E

R

R

K

W

I

Y

S

Q

A

A

G

M

I

M

R

D

E

N

R

L

A

D

T

D

E

L

I

A

S

L

A

I

L

M

I

T

V

C

E

E

E

Q

G

G

G

K

K

P

I

L

Q

S
x
Q

E
x
L

A

A

K

E

G
x
V

E

E

I

V

R

A

Y
x
F

G

T

A

A

G

D

F

Y

A

I

K

D

W

Y

F

M

A

A

E

E

E

W

A

A

R

R

V

Y

Y

E

G

G

E

E

T

I

I

I

P

Q

A

S

P

D

S

R

G

P

D

G

R

E

R

N

L

I

L

L

T

L

L

F

K

K

Q

R

P

A

V

L

G

G

V

V

C

T

A

T

A

G

I

I

T

L

P

P

W

W

N
|
N

F
|
F

P

P

N

F

A
x
F

M

L

I

I

T

A

R

R

K

K

C

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

P

T

V

I

I

V

V

I

K
|
K

P

P

S

S

D

E

L

F

T

T

P

P

L

N

S

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

V

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

N

N

V

L

V

V

T

L

G

G

M

R

P

G

A

E

G

T

I

V

G

G

E

Q

E

E

L

L

T

A

G

G

N

N

P

P

T

K

V

V

R

A

K

M

I

V

S

S

F

M

T

T

G

G

S

S

T

V

A

S

V

A

G

G

R

E

L

K

L

I

M

M

R

A

Q

T

S

A

A

A

E

K

H

N

I

I

K

T

R

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

D

D

L

L

E

E

Q

L

A

A

V

V

A

K

G

A

I

I

M

V

L
x
D

S

S

K

R

F

V

R
x
I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V
x
N

C

C

A

A

N

E

R

R

I

V

L

Y

V

V

Q

Q

D

K

G

G

I

I

Y

Y

E

D

R

Q

F

F

A

V

A

N

R

R

L

L

V

G

E

E

E

A

V

M

G

Q

K

A

L

V

K

Q

V
x
F

G
|
G

N

N

G

P

L

A

E

E

A
x
R

G

N

-
x
D

V
x
I

M
x
A

I

M

G

G

P

P

L

L

I

I

N

N

L

A

A

A

V

L

N

E

K

R

V

V

A

E

R

Q

H

K

I

V

D

A

D

R

A

A

L

V

S

E

Q

E

G

G

A

A

R

R

L

V

L

A

C

L

G

G

-

K

V

A

P

V

E

E

G

G

D
x
K

S

G

Q

Y

F
x
Y

V

Y

Q

P

P

P

T

T

V

L

L

L

G

L

E

D

A

V

H

R

A

Q

G

E

M

M

L

S

L

I

A

M

N

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

V

A

L

P

P

L

V

M

V

R

A

F

F

T

D

T

T

E

L

E

E

Q

E

A

A

L

I

A

S

L

M

A

A

N

N

A

D

T

S

P

D

Y

Y

G

G

L

L

G

T

A

S

Y

S

Y

I

F

Y

T

T

Q

Q

D

N

L

L

R

N

R

V

S

A

W

M

R

K

F

A

G

I

E

K

A

G

L

L

E

K

F

F

G

G

M

E

V

T

G

Y

L

I

N

N

T

R

G

E

I

N

I
x
F

S
x
E

M

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

I

W

K

R

Q

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

L

L

D

H

E

E

Y

Y

L

L

E

Q

V

T

K

Q

A

V

F

V

H

Y

I

L

active site: N151 (= N160), K174 (= K183), E249 (= E258), C283 (= C292), E381 (= E388), A458 (≠ E465)
binding (3alpha,5beta,12alpha)-3,12-dihydroxycholan-24-oic acid: Q97 (≠ S106), L98 (≠ E107), V101 (≠ G110), F105 (≠ Y114), F152 (= F161), F155 (≠ A164), D273 (≠ L282), I277 (≠ R286), N284 (≠ V293), F312 (≠ V321), G313 (= G322), R318 (≠ A327), D320 (vs. gap), I321 (≠ V329), A322 (≠ M330), K359 (≠ D366), Y362 (≠ F369), F440 (≠ I447), F440 (≠ I447), E441 (≠ S448)

P25553 Lactaldehyde dehydrogenase; Aldehyde dehydrogenase A; Glycolaldehyde dehydrogenase; EC 1.2.1.22; EC 1.2.1.21 from Escherichia coli (strain K12) (see 5 papers)
40% identity, 96% coverage: 17:482/485 of query aligns to 10:477/479 of P25553

query
sites
P25553

Y

Y

V

I

D

D

G

G

Q

Q

W

F

I

V

G

T

A

W

D

R

N

G

A

D

A

A

T

W

L

I

D

D

V

V

I

V

D

N

P

P

A

A

T

T

G

E

H

A

S

V

L

I

A

S

R

R

V

I

P

P

A

D

M

G

Q

Q

G

A

V

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

R

L

P

P

A

A

A

I

E

E

R

R

A

A

A

S

L

W

L

L

E

R

R

K

W

I

Y

S

Q

A

A

G

M

I

M

R

D

E

N

R

L

A

D

S

D

E

L

I

A

S

L

A

I

L

M

I

T

V

C

E

E

E

Q

G

G

G

K

K

P

I

L

Q

S

Q

E

L

A

A

K

E

G

V

E

E

I

V

R

A

Y

F

G

T

A

A

G

D

F

Y

A

I

K

D

W

Y

F

M

A

A

E

E

E

W

A

A

R

R

V

Y

Y

E

G

G

E

E

T

I

I

I

P

Q

A

S

P

D

S

R

G

P

D

G

R

E

R

N

L

I

L

L

T

L

L

F

K

K

Q

R

P

A

V

L

G

G

V

V

C

T

A

T

A

G

I

I

T
x
L

P

P

W

W

N

N

F

F

P

P

N

F

A

F

M

L

I

I

T

A

R
|
R

K

K

C

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

P

T

V

I

I

V

V

I

K
|
K

P
|
P

S
|
S

D
x
E

L
x
F

T

T

P

P

L

N

S

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

V

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

N

N

V

L

V

V

T

L

G

G

M

R

P

G

A

E

G

T

I

V

G

G

E
x
Q

E

E

L

L

T

A

G

G

N

N

P

P

T

K

V

V

R

A

K

M

I

V

S

S

F

M

T

T

G

G

S
|
S

T

V

A

S

V

A

G

G

R

E

L

K

L

I

M

M

R

A

Q

T

S

A

A

A

E

K

H

N

I

I

K

T

R

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

D

D

L

L

E

E

Q

L

A

A

V

V

A

K

G

A

I

I

M

V

L

D

S

S

K

R

F

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C

C

V
x
N

C

C

A

A

N

E

R

R

I

V

L

Y

V

V

Q

Q

D

K

G

G

I

I

Y

Y

E

D

R

Q

F

F

A

V

A

N

R

R

L

L

V

G

E

E

E

A

V

M

G

Q

K

A

L

V

K

Q

V

F

G

G

N

N

G

P

L

A

E

E

A

R

G

N

-

D

V

I

M

A

I

M

G

G

P

P

L

L

I

I

N

N

L

A

A

A

V

L

N

E

K
x
R

V

V

A

E

R

Q

H

K

I

V

D

A

D

R

A

A

L

V

S

E

Q

E

G

G

A

A

R

R

L

V

L

A

C

F

G

G

-

K

V

A

P

V

E

E

G

G

D

K

S

G

Q

Y

F

Y

V

Y

Q

P

P

P

T

T

V

L

L

L

G

L

E

D

A

V

H

R

A

Q

G

E

M

M

L

S

L

I

A

M

N

H

E

E

T

T

F

F

G

G

P

P

V

V

A

L

P

P

L

V

M

V

R

A

F

F

T

D

T

T

E

L

E

E

Q

D

A

A

L

I

A

S

L

M

A

A

N

N

A

D

T

S

P

D

Y

Y

G

G

L

L

G

T

A

S

Y

S

Y

I

F

Y

T

T

Q

Q

D

N

L

L

R

N

R

V

S

A

W

M

R

K

F

A

G

I

E

K

A

G

L

L

E

K

F

F

G

G

M

E

V

T

G

Y

L

I

N

N

T

R

G

E

I

N

I

F

S
x
E

M

A

E

M

V

Q

A

G

P

F

F
x
H

G

A

G

G

I

W

K

R

Q

K

S

S

G

G

L

I

G

G

R

G

E

A

G

D

S

G

K

K

Y

H

G

G

L

L

D

H

E

E

Y

Y

L

L

E

Q

V

T

K

Q

A

V

F

V

H

Y

I

L

L150 (≠ T157) binding
R161 (= R168) binding
KPSE 176:179 (≠ KPSD 183:186) binding
F180 (≠ L187) mutation to T: Can bind and use NADP(+) as coenzyme. 16-fold increase in catalytic efficiency with NAD(+) as coenzyme.
Q214 (≠ E221) binding
S230 (= S237) binding
E251 (= E258) binding
N286 (≠ V293) binding ; mutation to E: 4-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to H: 15-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.; mutation to T: 6-fold increase in catalytic efficiency with L-lactaldehyde as substrate. Shows expanded substrate specificity.
R336 (≠ K342) binding
E443 (≠ S448) binding
H449 (≠ F454) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2impA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the ternary complex with lactate (occupancy 0.5) and nadh. Crystals soaked with (l)-lactate. (see paper)
40% identity, 96% coverage: 17:482/485 of query aligns to 8:475/477 of 2impA

query
sites
2impA

Y

Y

V

I

D

D

G

G

Q

Q

W

F

I

V

G

T

A

W

D

R

N

G

A

D

A

A

T

W

L

I

D

D

V

V

I

V

D

N

P

P

A

A

T

T

G

E

H

A

S

V

L

I

A

S

R

R

V

I

P

P

A

D

M

G

Q

Q

G

A

V

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

R

L

P

P

A

A

A

I

E

E

R

R

A

A

A

S

L

W

L

L

E

R

R

K

W

I

Y

S

Q

A

A

G

M

I

M

R

D

E

N

R

L

A

D

S

D

E

L

I

A

S

L

A

I

L

M

I

T

V

C

E

E

E

Q

G

G

G

K

K

P

I

L

Q

S

Q

E

L

A

A

K

E

G

V

E

E

I

V

R

A

Y

F

G

T

A

A

G

D

F

Y

A

I

K

D

W

Y

F

M

A

A

E

E

E

W

A

A

R

R

V

Y

Y

E

G

G

E

E

T

I

I

I

P

Q

A

S

P

D

S

R

G

P

D

G

R

E

R

N

L

I

L

L

T

L

L

F

K

K

Q

R

P

A

V

L

G

G

V

V

C

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

N
|
N

F

F

P

P

N

F

A

F

M

L

I

I

T

A

R

R

K

K

C

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

P

T

V

I

I

V

V

I

K
|
K

P
|
P

S
|
S

D
x
E

L
x
F

T

T

P

P

L

N

S

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

V

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

N

N

V

L

V

V

T

L

G

G

M

R

P
x
G

A
x
E

G

T

I

V

G
|
G

E
x
Q

E

E

L

L

T

A

G

G

N

N

P

P

T

K

V

V

R

A

K

M

I

V

S

S

F
x
M

T

T

G
|
G

S
|
S

T

V

A

S

V
x
A

G

G

R

E

L
x
K

L
x
I

M

M

R

A

Q

T

S

A

A

A

E

K

H

N

I

I

K

T

R

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

D

D

L

L

E

E

Q

L

A

A

V

V

A

K

G

A

I

I

M

V

L

D

S

S

K

R

F

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V

N

C

C

A

A

N

E

R

R

I

V

L

Y

V

V

Q

Q

D

K

G

G

I

I

Y

Y

E

D

R

Q

F

F

A

V

A

N

R

R

L

L

V

G

E

E

E

A

V

M

G

Q

K

A

L

V

K

Q

V

F

G

G

N

N

G

P

L

A

E

E

A

R

G

N

-

D

V

I

M

A

I

M

G

G

P

P

L

L

I

I

N
|
N

L

A

A
|
A

A

A

V

L

N

E

K
x
R

V

V

A

E

R

Q

H

K

I

V

D

A

D

R

A

A

L

V

S

E

Q

E

G

G

A

A

R

R

L

V

L

A

C

F

G

G

-

K

V

A

P

V

E

E

G

G

D

K

S

G

Q

Y

F

Y

V

Y

Q

P

P

P

T

T

V

L

L

L

G

L

E

D

A

V

H

R

A

Q

G

E

M

M

L

S

L

I

A

M

N

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

V

A

L

P

P

L

V

M

V

R

A

F

F

T

D

T

T

E

L

E

E

Q

D

A

A

L

I

A

S

L

M

A

A

N

N

A

D

T

S

P

D

Y

Y

G

G

L

L

G

T

A

S

Y

S

Y

I

F

Y

T

T

Q

Q

D

N

L

L

R

N

R

V

S

A

W

M

R

K

F

A

G

I

E

K

A

G

L

L

E

K

F

F

G

G

M

E

V

T

G

Y

L

I

N

N

T

R

G

E

I

N

I

F

S

E

M

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

I

W

K

R

Q

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

L

L

D

H

E

E

Y

Y

L

L

E

Q

V

T

K

Q

A

V

F

V

H

Y

I

L

active site: N151 (= N160), K174 (= K183), E249 (= E258), C283 (= C292), E381 (= E388), A458 (≠ E465)
binding 1,4-dihydronicotinamide adenine dinucleotide: I147 (= I156), L148 (≠ T157), P149 (= P158), W150 (= W159), K174 (= K183), P175 (= P184), S176 (= S185), E177 (≠ D186), F178 (≠ L187), G207 (≠ P216), E208 (≠ A217), G211 (= G220), Q212 (≠ E221), M225 (≠ F234), G227 (= G236), S228 (= S237), A231 (≠ V240), K234 (≠ L243), I235 (≠ L244), N328 (= N336), A330 (= A338), R334 (≠ K342)

2iluA Crystal structure of lactaldehyde dehydrogenase from e. Coli: the binary complex with NADPH (see paper)
40% identity, 96% coverage: 17:482/485 of query aligns to 8:475/477 of 2iluA

query
sites
2iluA

Y

Y

V

I

D

D

G

G

Q

Q

W

F

I

V

G

T

A

W

D

R

N

G

A

D

A

A

T

W

L

I

D

D

V

V

I

V

D

N

P

P

A

A

T

T

G

E

H

A

S

V

L

I

A

S

R

R

V

I

P

P

A

D

M

G

Q

Q

G

A

V

E

E

D

T

A

R

R

R

K

A

A

I

I

E

D

A

A

E

E

R

R

A

A

W

Q

P

P

A

E

W

W

R

E

A

A

R

L

P

P

A

A

A

I

E

E

R

R

A

A

A

S

L

W

L

L

E

R

R

K

W

I

Y

S

Q

A

A

G

M

I

M

R

D

E

N

R

L

A

D

S

D

E

L

I

A

S

L

A

I

L

M

I

T

V

C

E

E

E

Q

G

G

G

K

K

P

I

L

Q

S

Q

E

L

A

A

K

E

G

V

E

E

I

V

R

A

Y

F

G

T

A

A

G

D

F

Y

A

I

K

D

W

Y

F

M

A

A

E

E

E

W

A

A

R

R

V

Y

Y

E

G

G

E

E

T

I

I

I

P

Q

A

S

P

D

S

R

G

P

D

G

R

E

R

N

L

I

L

L

T

L

L

F

K

K

Q

R

P

A

V

L

G

G

V

V

C

T

A

T

A

G

I
|
I

T
x
L

P
|
P

W
|
W

N
|
N

F

F

P

P

N

F

A

F

M

L

I

I

T

A

R

R

K

K

C

M

A

A

P

P

A

A

L

L

A

L

A

T

G

G

C

N

P

T

V

I

I

V

V

I

K
|
K

P

P

S
|
S

D
x
E

L
x
F

T

T

P

P

L

N

S

N

A

A

L

I

A

A

L

F

A

A

V

K

L

I

A

V

E

D

R

E

V

I

G

G

I

L

P

P

A

R

G

G

V

V

F

F

N

N

V

L

V

V

T

L

G

G

M
x
R

P
x
G

A
x
E

G

T

I

V

G
|
G

E
x
Q

E

E

L

L

T

A

G

G

N

N

P

P

T

K

V

V

R

A

K

M

I

V

S

S

F
x
M

T

T

G
|
G

S
|
S

T

V

A

S

V
x
A

G

G

R

E

L
x
K

L
x
I

M

M

R

A

Q

T

S

A

A

A

E

K

H

N

I

I

K

T

R

K

L

V

S

C

L

L

E
|
E

L

L

G

G

N

K

A

A

P

P

F

A

I

I

V

V

F

M

D

D

A

A

D

D

L

L

E

E

Q

L

A

A

V

V

A

K

G

A

I

I

M

V

L

D

S

S

K

R

F

V

R

I

N

N

A

S

G

G

Q

Q

T

V

C
|
C

V

N

C

C

A

A

N

E

R

R

I

V

L

Y

V

V

Q

Q

D

K

G

G

I

I

Y

Y

E

D

R

Q

F

F

A

V

A

N

R

R

L

L

V

G

E

E

E

A

V

M

G

Q

K

A

L

V

K

Q

V

F

G

G

N

N

G

P

L

A

E

E

A

R

G

N

-

D

V

I

M

A

I

M

G

G

P

P

L

L

I

I

N
|
N

L

A

A

A

V

L

N

E

K
x
R

V

V

A

E

R

Q

H

K

I

V

D

A

D

R

A

A

L

V

S

E

Q

E

G

G

A

A

R

R

L

V

L

A

C

F

G

G

-

K

V

A

P

V

E

E

G

G

D

K

S

G

Q

Y

F

Y

V

Y

Q

P

P

P

T

T

V

L

L

L

G

L

E

D

A

V

H

R

A

Q

G

E

M

M

L

S

L

I

A

M

N

H

E

E

E
|
E

T

T

F
|
F

G

G

P

P

V

V

A

L

P

P

L

V

M

V

R

A

F

F

T

D

T

T

E

L

E

E

Q

D

A

A

L

I

A

S

L

M

A

A

N

N

A

D

T

S

P

D

Y

Y

G

G

L

L

G

T

A

S

Y

S

Y

I

F

Y

T

T

Q

Q

D

N

L

L

R

N

R

V

S

A

W

M

R

K

F

A

G

I

E

K

A

G

L

L

E

K

F

F

G

G

M

E

V

T

G

Y

L

I

N

N

T

R

G

E

I

N

I

F

S

E

M

A

E

M

V

Q

A

G

P

F

F

H

G

A

G

G

I

W

K

R

Q

K

S

S

G

G

L

I

G

G

R

G

E
x
A

G

D

S

G

K

K

Y

H

G

G

L

L

D

H

E

E

Y

Y

L

L

E

Q

V

T

K

Q

A

V

F

V

H

Y

I

L

active site: N151 (= N160), K174 (= K183), E249 (= E258), C283 (= C292), E381 (= E388), A458 (≠ E465)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I147 (= I156), L148 (≠ T157), P149 (= P158), W150 (= W159), K174 (= K183), S176 (= S185), E177 (≠ D186), F178 (≠ L187), R206 (≠ M215), G207 (≠ P216), E208 (≠ A217), G211 (= G220), Q212 (≠ E221), M225 (≠ F234), G227 (= G236), S228 (= S237), A231 (≠ V240), K234 (≠ L243), I235 (≠ L244), N328 (= N336), R334 (≠ K342), F383 (= F390)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
41% identity, 96% coverage: 18:482/485 of query aligns to 9:474/476 of 5x5uA

query
sites
5x5uA

V

I

D

D

G

G

Q

E

W

W

I

V

G
x
D

A
|
A

D
x
A

N

S

A
x
G

A

K

T

T

L

I

D

D

V

V

I

V

D

N

P

P

A

A

T

T

G

G

H

K

S

P

L

I

A

G

R

R

V

V

P

A

A

H

M

A

Q

G

G

I

V

A

E

D

T

L

R

D

R

R

A

A

I

L

E

A

A

A

E

Q

R

S

A

G

W

F

P

E

A

A

W

W

R

R

A

K

R

V

P

P

A

A

A

H

E

E

R

R

A

A

L

T

L

M

E

R

R

K

W

A

Y

A

Q

A

A

L

M

V

M

R

D

E

N

R

L

A

D

D

D

A

L

I

A

A

L

Q

I

L