SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_3805 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3805 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

P25080 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 4 papers)
92% identity, 98% coverage: 10:561/562 of query aligns to 6:557/557 of P25080

query
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P25080

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GG 53:54 (= GG 57:58) binding
C64 (= C68) mutation to A: No loss of activity.
Q131 (= Q135) binding
GMG 177:179 (= GMG 181:183) binding
C192 (= C196) mutation to A: No loss of activity.
ECQQSR 197:202 (≠ ECQQVS 201:206) binding
C198 (= C202) mutation to A: No loss of activity.
NA 243:244 (= NA 247:248) binding
QTSAH 264:268 (= QTSAH 268:272) binding
YL 274:275 (= YL 278:279) binding
YG 323:324 (= YG 327:328) binding
C355 (= C359) mutation to A: Minor loss in activity.
C411 (= C415) mutation to A: Loss of activity.
RE 455:456 (= RE 459:460) binding
G493 (= G497) binding
C544 (= C548) mutation to A: No loss of activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1uwkA The high resolution structure of urocanate hydratase from pseudomonas putida in complex with urocanate (see paper)
92% identity, 98% coverage: 10:561/562 of query aligns to 3:554/554 of 1uwkA

query
sites
1uwkA

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binding nicotinamide-adenine-dinucleotide: Y49 (= Y56), G50 (= G57), G51 (= G58), I142 (= I149), G173 (= G180), G174 (= G181), M175 (= M182), G176 (= G183), E194 (= E201), S195 (≠ C202), Q196 (= Q203), N240 (= N247), A241 (= A248), Q261 (= Q268), T262 (= T269), S263 (= S270), H265 (= H272), Y271 (= Y278), L272 (= L279), W278 (= W285), Y320 (= Y327), G321 (= G328), N322 (= N329), F342 (= F349), G490 (= G497)
binding (2e)-3-(1h-imidazol-4-yl)acrylic acid: Y49 (= Y56), M129 (= M136), T130 (= T137), G141 (= G148), M175 (= M182), R359 (= R366), D440 (= D447)

7jfzA Structure of urocanate hydratase from legionella pneumophila bound to NAD
72% identity, 97% coverage: 16:558/562 of query aligns to 3:545/547 of 7jfzA

query
sites
7jfzA

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T

Y

Y

E

E

T

T

F

F

V

V

E

A

A

A

G

A

R

K

Q

K

H

H

Y

Y

D

Q

S

G

N

D

L

L

K

S

G

G

R

R

W

W

V

I

L

L

T

T

A

A

G

G

L

L

G
|
G

M
|
M

G

G

A

A

Q

Q

P

P

L

L

A

A

A

G

T

T

L

M

A

A

G

G

A

A

C

S

S

V

L

L

N

A

I

V

E
|
E

C
|
C

Q

D

Q

R

V

Q

S
x
R

I

I

D

E

F

K

R

R

L

L

K

Q

T

T

R

K

Y

Y

V

L

D

D

E

R

Q

Y

A

T

T

D

D

N

L

L

D

S

D

E

A

A

L

L

A

D

R

W

I

I

E

N

K

E

Y

S

T

C

A

R

E

R

G

K

K

K

A

P

I

V

S

S

I

V

A

A

L

V

C

L

G

G

N
|
N

A
|
A

A

A

E

E

I

I

L

F

P

P

E

Q

M

L

V

V

R

K

R

L

G

G

V

V

R

Q

P

P

D

S

M

L

V

V

T

T

D

D

Q
|
Q

T
|
T

S
|
S

A

A

H
|
H

D

D

P

P

L

L

N

N

G

G

Y
|
Y

L
|
L

P

P

A

L

G

G

W

W

T

T

W

L

D

E

E

Q

Y

A

R

V

A

E

R

M

A

R

K

K

T

K

E

S

P

P

A

E

V

V

K

D

A

A

K

K

Q

K

S

S

M

M

A

A

I

V

H

Q

V

V

K

H

A

A

M

M

L

L

A

E

F

F

Q

H

K

N

M

R

G

G

V

I

P

P

T

V

F

F

D

D

Y
|
Y

G
|
G

N
|
N

N

N

I

I

R

R

Q

Q

M

M

A

A

Q

F

E

E

E

A

G

G

V

E

E

K

N

N

A

A

F

F

D

S

F

F

P

E

G

G

F
|
F

V

V

P

P

A

A

Y

Y

I

I

R

R

P

P

L

L

F

F

C

C

R

E

G

G

I

I

G

G

P

P

F

F

R

R

W

W

A

V

A

A

L

L

S

S

G

G

D

D

P

P

Q

E

D

D

I

I

Y

Y

K

A

T

T

D

D

A

E

K

R

V

V

K

K

E

Q

L

L

I

I

P

P

D

D

D

A

A

P

H

H

L

L

H

H

N

H

W

W

L

L

D

D

M

M

A

A

R

R

E

E

R

K

I

I

S

S

F

F

Q

Q

G

G

L

L

P

P

A

A

R

R

I

I

C

C

W

W

V

V

G

G

L

L

G

K

Q

D

R

R

A

A

K

R

L

L

G

A

L

L

A

A

F

F

N

N

E

E

M

M

V

V

R

K

S

N

G

K

E

Q

L

V

S

K

A

A

P

P

I

I

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

D

S

S

G

G

S

S

V

V

A

A

S

S

P

P

N

N

R
|
R

E

E

T

T

E

E

S

G

M

M

Q

L

D

D

G

G

S

S

D

D

A

A

V

V

S

S

D

D

W

W

P

P

L

L

N

N

A

A

L

L

N

N

T

C

A

A

S

S

G

G

A

A

T

T

W

W

V

V

S

S

L

I

H

H

G

G

V

V

G

G

M

M

G

G

F

F

S

S

Q

Q

H

H

S

A

G

G

M

V

V

V

I

I

V

V

C

A

D

D

G

G

T

T

D

E

E

K

A

A

E

K

R

R

I

L

A

A

R

R

V

V

L

L

H

H

N

N

D

D

P

P

G

A

T

T

G

G

V

V

M

M

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

Q

Q

I

I

A

A

I

K

D

Q

C

C

A

A

K

K

E

E

Q

N

G

S

L

L

N

W

L

L

P

P

M

M

binding nicotinamide-adenine-dinucleotide: G167 (= G180), G168 (= G181), M169 (= M182), E188 (= E201), C189 (= C202), R193 (≠ S206), N234 (= N247), A235 (= A248), Q255 (= Q268), T256 (= T269), S257 (= S270), H259 (= H272), Y265 (= Y278), L266 (= L279), Y314 (= Y327), G315 (= G328), N316 (= N329), F336 (= F349), R446 (= R459)

P25503 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Bacillus subtilis (strain 168)
64% identity, 97% coverage: 16:558/562 of query aligns to 8:550/552 of P25503

query
sites
P25503

I

I

R

R

A

A

P

N

R

R

G

G

N

T

T

E

L

L

T

E

A

C

K

L

S

G

W

W

L

E

T

Q

E

E

A

A

P

V

L

L

R

R

M

M

L

L

M

R

N

N

L

L

D

D

P

P

E

E

V

V

A

A

E

E

N

K

P

P

K

E

E

D

L

L

V

I

V

V

Y

Y

G
|
G

I

I

G

G

R

K

A

A

R

R

N

D

W

W

E

D

C

A

Y

F

D

H

K

A

I

I

V

E

E

H

S

S

L

L

T

K

N

T

L

L

N

K

D

N

D

D

E

E

T

T

L

L

V

V

Q

Q

S

S

G

G

K

K

P

P

V

V

G

G

V

M

F

F

K

R

T

T

H

H

S

P

N

Q

A

A

P

P

R

R

V

V

L

L

I

L

A

A

N

N

S

S

N

V

L

L

V

V

P

P

H

K

W

W

A

A

S

D

W

W

E

E

H

H

F

F

N

H

E

E

L

L

D

E

A

K

K

K

G

G

L

L

A

M

M

M

Y

Y

G

G

Q
|
Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I

I

V

L

Q

Q

G

G

T

T

Y

Y

E

E

T

T

F

F

V

A

E

E

A

L

G

A

R

R

Q

Q

H

H

Y

F

D

G

S

G

N

S

L

L

K

K

G

G

R

T

W

L

V

T

L

L

T

T

A

A

G

G

L

L

G

G

G
|
G

M
|
M

G
|
G

G

G

A

A

Q

Q

P

P

L

L

A

S

A

V

T

T

L

M

A

N

G

E

A

G

C

V

S

V

L

I

N

A

I

V

E
|
E

C

V

Q

D

Q

E

V

K

S
x
R

I

I

D

D

F

K

R

R

L

I

K

E

T

T

R

K

Y

Y

V

C

D

D

E

R

Q

K

A

T

T

A

D

S

L

I

D

E

A

A

L

L

A

A

R

W

I

A

E

E

K

E

Y

A

T

K

A

L

E

A

G

G

K

K

A

P

I

L

S

S

I

I

A

A

L

L

C

L

G

G

N
|
N

A
|
A

A

A

E

E

I

V

L

H

P

H

E

T

M

L

V

L

R

N

R

R

G

G

V

V

R

K

P

I

D

D

M

I

V

V

T

T

D

D

Q
|
Q

T
|
T

S
|
S

A
|
A

H
|
H

D

D

P

P

L

L

N

I

G

G

Y
|
Y

L
x
V

P

P

A

E

G

G

W

Y

T

S

W

L

D

D

E

E

Y

A

R

D

A

R

R

L

A

R

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Q

T

D

E

T

P

P

A

E

A

L

V

Y

V

V

K

R

A

L

A

A

K

K

Q

Q

S

S

M

M

A

K

I

K

H

H

V

V

K

E

A

A

M

M

L

L

A

A

F

F

Q

Q

K

Q

M

K

G

G

V

S

P

I

T

V

F

F

D

D

Y
|
Y

G

G

N

N

I

I

R

R

Q

Q

M

V

A

A

Q

K

E

D

E

E

G

G

V

L

E

E

N

N

A

A

F

F

D

D

F

F

P

P

G

G

F

F

V

V

P

P

A

A

Y

Y

I

I

R

R

P

P

L

L

F

F

C

C

R

E

G

G

I

K

G

G

P

P

F

F

R

R

W

W

A

A

L

L

S

S

G

G

D

D

P

P

Q

A

D

D

I

I

Y

Y

K

R

T

T

D

D

A

A

K

L

V

L

K

K

E

E

L

L

I

F

P

P

D

T

D

N

A

K

H

A

L

L

H

H

N

R

W

W

L

I

D

D

M

M

A

A

R

Q

E

E

R

K

I

V

S

T

F

F

Q

Q

G

G

L

L

P

P

A

S

R

R

I

I

C

C

W

W

V

L

G

G

L

Y

G

G

Q

E

R

R

A

K

K

K

L

M

G

G

L

L

A

A

F

I

N

N

E

E

M

L

V

V

R

R

S

T

G

G

E

E

L

L

S

K

A

A

P

P

I

V

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

D

S

C

G

G

S

S

V

V

A

A

S

S

P

P

N

N

R

R

E

E

T

T

E

E

S

A

M

M

Q

K

D

D

G

G

S

S

D

D

A

A

V

V

S

G

D

D

W

W

P

A

L

V

L

L

N

N

A

A

L

L

L

V

N

N

T

T

A

A

S

A

G

G

A

A

T

S

W

W

V

V

S

S

L

F

H

H

G

G

G
|
G

V

V

G

G

M

M

G

G

F

Y

S

S

Q

L

H

H

S

A

G

G

M

M

V

V

I

A

V

V

C

A

D

D

G

G

T

S

D

E

E

L

A

A

A

D

E

E

R

R

I

L

A

A

R

R

V

V

L

L

H

T

N

S

D

D

P

P

G

G

T

M

G

G

V

I

M

I

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

Q

E

I

R

A

A

I

V

D

E

C

V

A

A

K

K

E

E

Q

Q

G

D

L

I

N

I

L

V

P

P

M

M

GG 49:50 (= GG 57:58) binding
Q127 (= Q135) binding
GMG 173:175 (= GMG 181:183) binding
E193 (= E201) binding
R198 (≠ S206) binding
NA 239:240 (= NA 247:248) binding
QTSAH 260:264 (= QTSAH 268:272) binding
YV 270:271 (≠ YL 278:279) binding
Y319 (= Y327) binding
G489 (= G497) binding

2fknB Crystal structure of urocanase from bacillus subtilis
64% identity, 97% coverage: 16:558/562 of query aligns to 2:544/546 of 2fknB

query
sites
2fknB

I

I

R

R

A

A

P

N

R

R

G

G

N

T

T

E

L

L

T

E

A

C

K

L

S

G

W

W

L

E

T

Q

E

E

A

A

P

V

L

L

R

R

M

M

L

L

M

R

N

N

L

L

D

D

P

P

E

E

V

V

A

A

E

E

N

K

P

P

K

E

E

D

L

L

V

I

V

V

Y
|
Y

G
|
G

I

I

G

G

R

K

A

A

R

R

N

D

W

W

E

D

C

A

Y

F

D

H

K

A

I

I

V

E

E

H

S

S

L

L

T

K

N

T

L

L

N

K

D

N

D

D

E

E

T

T

L

L

V

V

Q

Q

S

S

G

G

K

K

P

P

V

V

G

G

V

M

F

F

K

R

T

T

H

H

S

P

N

Q

A

A

P

P

R

R

V

V

L

L

I

L

A

A

N

N

S

S

N

V

L

L

V

V

P

P

H

K

W

W

A

A

S

D

W

W

E

E

H

H

F

F

N

H

E

E

L

L

D

E

A

K

K

K

G

G

L

L

A

M

M

M

Y

Y

G

G

Q

Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I
|
I

V

L

Q

Q

G

G

T

T

Y

Y

E

E

T

T

F

F

V

A

E

E

A

L

G

A

R

R

Q

Q

H

H

Y

F

D

G

S

G

N

S

L

L

K

K

G

G

R

T

W

L

V

T

L

L

T

T

A

A

G

G

L

L

G
|
G

M
|
M

G

G

A

A

Q

Q

P

P

L

L

A

S

A

V

T

T

L

M

A

N

G

E

A

G

C

V

S

V

L

I

N

A

I

V

E
|
E

C
x
V

Q

D

Q

E

V

K

S
x
R

I

I

D

D

F

K

R

R

L

I

K

E

T

T

R

K

Y

Y

V

C

D

D

E

R

Q

K

A

T

T

A

D

S

L

I

D

E

A

A

L

L

A

A

R

W

I

A

E

E

K

E

Y

A

T

K

A

L

E

A

G

G

K

K

A

P

I

L

S

S

I

I

A

A

L

L

C

L

G

G

N
|
N

A
|
A

A

A

E

E

I

V

L

H

P

H

E

T

M

L

V

L

R

N

R

R

G

G

V

V

R

K

P

I

D

D

M

I

V

V

T

T

D

D

Q
|
Q

T
|
T

S
|
S

A

A

H
|
H

D

D

P

P

L

L

N

I

G

G

Y
|
Y

L
x
V

P

P

A

E

G

G

W

Y

T

S

W

L

D

D

E

E

Y

A

R

D

A

R

R

L

A

R

K

Q

T

D

E

T

P

P

A

E

A

L

V

Y

V

V

K

R

A

L

A

A

K

K

Q

Q

S

S

M

M

A

K

I

K

H

H

V

V

K

E

A

A

M

M

L

L

A

A

F

F

Q

Q

K

Q

M

K

G

G

V

S

P

I

T

V

F

F

D

D

Y

Y

G

G

N
|
N

N

N

I

I

R

R

Q

Q

M

V

A

A

Q

K

E

D

E

E

G

G

V

L

E

E

N

N

A

A

F

F

D

D

F

F

P

P

G

G

F
|
F

V

V

P

P

A

A

Y

Y

I

I

R

R

P

P

L

L

F

F

C

C

R

E

G

G

I

K

G

G

P

P

F

F

R

R

W

W

A

A

L

L

S

S

G

G

D

D

P

P

Q

A

D

D

I

I

Y

Y

K

R

T

T

D

D

A

A

K

L

V

L

K

K

E

E

L

L

I

F

P

P

D

T

D

N

A

K

H

A

L

L

H

H

N

R

W

W

L

I

D

D

M

M

A

A

R

Q

E

E

R

K

I

V

S

T

F

F

Q

Q

G

G

L

L

P

P

A

S

R

R

I

I

C

C

W

W

V

L

G

G

L

Y

G

G

Q

E

R

R

A

K

K

K

L

M

G

G

L

L

A

A

F

I

N

N

E

E

M

L

V

V

R

R

S

T

G

G

E

E

L

L

S

K

A

A

P

P

I

V

V

V

I

I

G

G

R

R

D

D

H

H

L

L

D

D

S

C

G

G

S

S

V

V

A

A

S

S

P

P

N

N

R
|
R

E

E

T

T

E

E

S

A

M

M

Q

K

D

D

G

G

S

S

D

D

A

A

V

V

S

G

D

D

W

W

P

A

L

V

L

L

N

N

A

A

L

L

L

V

N

N

T

T

A

A

S

A

G

G

A

A

T

S

W

W

V

V

S

S

L

F

H

H

G

G

G
|
G

V

V

G

G

M

M

G

G

F

Y

S

S

Q

L

H

H

S

A

G

G

M

M

V

V

I

A

V

V

C

A

D

D

G

G

T

S

D

E

E

L

A

A

A

D

E

E

R

R

I

L

A

A

R

R

V

V

L

L

H

T

N

S

D

D

P

P

G

G

T

M

G

G

V

I

M

I

R

R

H

H

A

A

D

D

A

A

G

G

Y

Y

Q

E

I

R

A

A

I

V

D

E

C

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A

A

K

K

E

E

Q

Q

G

D

L

I

N

I

L

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M

M

binding nicotinamide-adenine-dinucleotide: Y42 (= Y56), G43 (= G57), G44 (= G58), I135 (= I149), G166 (= G180), G167 (= G181), M168 (= M182), E187 (= E201), V188 (≠ C202), R192 (≠ S206), N233 (= N247), A234 (= A248), Q254 (= Q268), T255 (= T269), S256 (= S270), H258 (= H272), Y264 (= Y278), V265 (≠ L279), N315 (= N329), F335 (= F349), R445 (= R459), G483 (= G497)

Q5L084 Urocanate hydratase; Urocanase; Imidazolonepropionate hydrolase; EC 4.2.1.49 from Geobacillus kaustophilus (strain HTA426)
65% identity, 97% coverage: 16:559/562 of query aligns to 7:550/551 of Q5L084

query
sites
Q5L084

I

V

R

S

A

P

P

P

R

A

G

G

N

T

T

E

L

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W

L

I

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E

A

A

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A

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M

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M

N

N

N

L

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D

H

P

P

E

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A

A

E

E

N

R

P

P

K

D

E

E

L

L

V

I

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Y

Y

G

G

I

I

G

G

R

K

A

A

R

R

N

N

W

W

E

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C

C

Y

Y

D

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K

A

I

I

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E

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R

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E

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N

D

D

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E

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T

L

L

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I

Q

Q

S

S

G

G

K

K

P

P

V

V

G

A

V

V

F

F

K

R

T

T

H

H

S

P

N

D

A

A

P

P

R

R

V

V

L

L

I

I

A

A

N

N

S

S

N

N

L

L

V

V

P

P

H

A

W

W

A

A

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W

W

E

D

H

H

F

F

N

H

E

E

L

L

D

D

A

K

K

K

G

G

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L

A

I

M

M

Y

Y

G

G

Q

Q

M

M

T

T

A

A

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

S

S

Q

Q

G

G

I

I

V

V

Q

Q

G

G

T

T

Y

Y

E

E

T

T

F

F

V

A

E

E

A

V

G

A

R

R

Q

Q

H

H

Y

F

D

G

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G

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T

L

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A

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I

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L

T

T

A

A

G

G

L

L

G

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G
|
G

M
|
M

G
|
G

G

G

A

A

Q

Q

P

P

L

L

A

A

A

V

T

T

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M

A

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G

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E

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x
R

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Y

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M

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D

D

S

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L

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R

M

I

A

E

K

K

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K

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K

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K

A

A

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I

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N
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N

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A

A

A

E

E

I

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G

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Q

T
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T

S
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S

A
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H
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H

D

D

P

P

L

L

N

N

G

G

Y
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Y

L
x
I

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A

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L

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A

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M

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Y

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G

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N

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I

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M

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P

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E

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W

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P

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I

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A

A

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L

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T

A

A

V

S

G

G

G

A

A

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S

W

W

V

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A

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M

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I

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A

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D

K

E

E

A

A

A

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E

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I

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A

E

R

R

V

V

L

L

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T

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T

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D

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P

G

G

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L

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G

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V

M

V

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R

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H

A

A

D

D

A

A

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I

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A

A

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I

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T

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M

I

L

GMG 172:174 (= GMG 181:183) binding
E192 (= E201) binding
R197 (≠ S206) binding
NA 238:239 (= NA 247:248) binding
QTSAH 259:263 (= QTSAH 268:272) binding
YI 269:270 (≠ YL 278:279) binding
Y318 (= Y327) binding

1x87A 2.4a x-ray structure of urocanase protein complexed with NAD
58% identity, 97% coverage: 16:559/562 of query aligns to 1:494/495 of 1x87A

query
sites
1x87A

I

V

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P

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G

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W

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A

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L

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R

M

M

L

L

M

N

N

N

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P

P

E

D

V

V

A

-

E

-

N

-

P

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-

E

-

L

-

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Y

-

G

-

I

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G

-

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A

A

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N

W

W

E

E

C

C

Y

Y

D

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K

A

I

I

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L

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N

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D

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G

G

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K

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P

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G

A

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V

F

F

K

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T

H

H

S

P

N

D

A

A

P

P

R

R

V

V

L

L

I

I

A

A

N

N

S

S

N

N

L

L

V

V

P

P

H

A

W

W

A

A

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T

W

W

E

D

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-

N

-

E

-

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K

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G

-

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-

A

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Y

-

G

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Q

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M

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T

-

A

-

G

G

S

S

W

W

I

I

Y

Y

I

I

G

G

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S

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Q

G

G

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I

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Q

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Y

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E

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A

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A

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A

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G

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I

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L

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T

A

A

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G
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G

M
|
M

G

G

A

A

Q

Q

P

P

L

L

A

A

A

V

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T

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M

A

N

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G

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C

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E

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x
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x
R

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A

A

A

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L

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M

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A

E

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Y

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G

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K

A

A

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S

I

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G

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|
N

A
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A

A

A

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L

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N

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x
I

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A

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G

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G

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Y

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N

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F

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A

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A

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Q

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P

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A

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A

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D

D

A

A

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A

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P

L

I

L

L

N

N

A

A

L

L

N

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T

A

A

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G

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A

A

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W

W

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H

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M

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F

Y

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A

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E

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A

A

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E

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T

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D

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P

G

G

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G

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V

M

I

R

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H

H

A

A

D

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A

A

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E

I

L

A

A

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G

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P

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M

M

I

L

binding nicotinamide-adenine-dinucleotide: G134 (= G180), G135 (= G181), M136 (= M182), E155 (= E201), V156 (≠ C202), R160 (≠ S206), N201 (= N247), A202 (= A248), Q222 (= Q268), T223 (= T269), H226 (= H272), Y232 (= Y278), I233 (≠ L279), Y281 (= Y327), G282 (= G328), N283 (= N329), F303 (= F349)

7nedA Thiourocanate hydratase from paenibacillus sp. Soil724d2 in complex with cofactor NAD+ and urocanate (see paper)
51% identity, 95% coverage: 16:547/562 of query aligns to 3:532/545 of 7nedA

query
sites
7nedA

I

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-

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N

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Y

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D

binding nicotinamide-adenine-dinucleotide: Y41 (= Y56), A42 (≠ G57), A43 (≠ G58), G165 (= G180), G166 (= G181), M167 (= M182), E186 (= E201), V187 (≠ C202), R191 (≠ S206), N232 (= N247), A233 (= A248), Q253 (= Q268), T254 (= T269), H257 (= H272), Y263 (= Y278), V264 (≠ L279), G313 (= G328), N314 (= N329), I444 (≠ R459), Y484 (≠ G499)
binding (2e)-3-(1h-imidazol-4-yl)acrylic acid: Y41 (= Y56), L121 (≠ M136), T122 (= T137), M167 (= M182), R351 (= R366), D432 (= D447)

6uekA Structure of urocanate hydratase from trypanosoma cruzi in complex with NAD+ (see paper)
36% identity, 95% coverage: 7:541/562 of query aligns to 82:629/660 of 6uekA

query
sites
6uekA

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