SitesBLAST
Comparing Pf6N2E2_3903 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3903 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 15 hits to proteins with known functional sites (download)
7qyrD Crystal structure of rimk from pseudomonas aeruginosa pao1 (see paper)
92% identity, 97% coverage: 1:293/301 of query aligns to 1:293/293 of 7qyrD
- binding adenosine-5'-diphosphate: V139 (= V139), Q177 (= Q177), E178 (= E178), Y179 (= Y179), I180 (= I180), D187 (= D187), F210 (= F210), R211 (= R211), S212 (= S212), L250 (= L250), M259 (= M259)
- binding : S7 (= S7), L12 (= L12), S14 (= S14), T15 (= T15), R64 (= R64), G66 (= G66), R189 (= R189), N262 (= N262), S264 (= S264), G266 (= G266)
5zctH The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
66% identity, 96% coverage: 1:290/301 of query aligns to 1:290/292 of 5zctH
- binding phosphoaminophosphonic acid-adenylate ester: K100 (= K100), V139 (= V139), K141 (= K141), V151 (= V151), Q177 (= Q177), E178 (= E178), Y179 (= Y179), I180 (= I180), D187 (= D187), F210 (= F210), R211 (= R211), S212 (= S212), N213 (= N213), R216 (= R216), L250 (= L250), M259 (= M259), E260 (= E260)
- binding magnesium ion: D248 (= D248), E260 (= E260)
5zctA The crystal structure of the poly-alpha-l-glutamate peptides synthetase rimk at 2.05 angstrom resolution. (see paper)
66% identity, 96% coverage: 1:290/301 of query aligns to 1:290/292 of 5zctA
- binding phosphoaminophosphonic acid-adenylate ester: K100 (= K100), V139 (= V139), K141 (= K141), G145 (= G145), T146 (= T146), Q147 (= Q147), G148 (= G148), V151 (= V151), Q177 (= Q177), E178 (= E178), Y179 (= Y179), I180 (= I180), F210 (= F210), R211 (= R211), S212 (= S212), N213 (= N213), L250 (= L250), M259 (= M259), E260 (= E260)
4iwxA Rimk structure at 2.85a (see paper)
66% identity, 96% coverage: 1:290/301 of query aligns to 3:292/294 of 4iwxA
- binding adenosine-5'-diphosphate: V141 (= V139), K143 (= K141), V153 (= V151), E180 (= E178), Y181 (= Y179), I182 (= I180), K183 (= K181), D189 (= D187), R205 (= R203), F212 (= F210), R213 (= R211), S214 (= S212), N215 (= N213), D250 (= D248), L252 (= L250), M261 (= M259), E262 (= E260)
- binding glutamic acid: R98 (= R96), R104 (= R102), Q107 (= Q105), L108 (= L106), R111 (= R109)
3vpbB Argx from sulfolobus tokodaii complexed with lysw/glu/adp/mg/zn/sulfate (see paper)
31% identity, 79% coverage: 52:289/301 of query aligns to 41:279/282 of 3vpbB
- binding adenosine-5'-diphosphate: K87 (= K100), I125 (≠ V139), K127 (= K141), G131 (= G145), S132 (≠ T146), W133 (≠ Q147), G134 (= G148), V137 (= V151), Q167 (= Q177), E168 (= E178), Y169 (= Y179), I170 (= I180), D176 (= D187), W199 (≠ F210), R200 (= R211), A201 (≠ S212), N202 (= N213), D237 (= D248), L239 (= L250), N249 (≠ M259), E250 (= E260)
- binding glutamic acid: W133 (≠ Q147), R178 (= R189), Y190 (≠ M201), R192 (= R203), N202 (= N213), V203 (≠ L214), A204 (≠ H215), E256 (≠ G266), F257 (≠ L267), K258 (≠ E268), G259 (= G269), F260 (≠ I270)
- binding magnesium ion: D237 (= D248), E250 (= E260)
- binding zinc ion: E250 (= E260), N252 (= N262)
Q970U6 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
31% identity, 79% coverage: 52:289/301 of query aligns to 41:279/282 of Q970U6
- D237 (= D248) binding
- E250 (= E260) binding ; binding
- N252 (= N262) binding
Q4JAP9 Alpha-aminoadipate--LysW ligase LysX; AAA--LysW ligase LysX; EC 6.3.2.43 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (see paper)
29% identity, 69% coverage: 80:286/301 of query aligns to 62:270/276 of Q4JAP9
- NT 253:254 (≠ GI 269:270) mutation to GF: Alters substrate specificity, so that glutamate is preferred over alpha-aminoadipate.
3vpdA Lysx from thermus thermophilus complexed with amp-pnp (see paper)
29% identity, 78% coverage: 51:285/301 of query aligns to 42:274/281 of 3vpdA
- binding phosphoaminophosphonic acid-adenylate ester: V127 (= V139), K129 (= K141), W135 (≠ Q147), G136 (= G148), L139 (≠ V151), Q169 (= Q177), E170 (= E178), Y171 (= Y179), V172 (≠ I180), K174 (≠ A183), D178 (= D187), R180 (= R189), R194 (= R203), W199 (≠ F210), I200 (≠ R211), T201 (≠ S212), D237 (= D248), F239 (≠ L250), N248 (≠ M259), E249 (= E260), N251 (= N262)
- binding butanoic acid: T203 (≠ L214), E255 (≠ G266), F256 (≠ L267), K257 (≠ E268), N258 (≠ G269), S259 (≠ I270)
5k2mG Bifunctional lysx/argx from thermococcus kodakarensis with lysw-gamma- aaa (see paper)
26% identity, 71% coverage: 71:285/301 of query aligns to 54:266/273 of 5k2mG
- binding adenosine-5'-diphosphate: K83 (= K100), V120 (= V139), K122 (= K141), G126 (= G145), S127 (≠ T146), W128 (≠ Q147), G129 (= G148), L132 (≠ V151), Q162 (= Q177), E163 (= E178), F164 (≠ Y179), V165 (≠ I180), K167 (≠ A183), D171 (= D187), R187 (= R203), W192 (≠ F210), I193 (≠ R211), T194 (≠ S212), N195 (= N213), D229 (= D248), F231 (≠ L250), N240 (≠ M259), E241 (= E260)
- binding magnesium ion: D229 (= D248), E241 (= E260)
- binding 2-aminohexanedioic acid: W128 (≠ Q147), R173 (= R189), Y175 (≠ F191), R187 (= R203), N195 (= N213), T196 (≠ L214), A197 (≠ H215), E247 (≠ G266), F248 (≠ L267), N250 (≠ G269), A251 (≠ I270)
- binding : F54 (= F71), F125 (≠ E144), S127 (≠ T146), W128 (≠ Q147), R130 (≠ I149), N153 (≠ Q172), Y156 (≠ M175), R173 (= R189), N243 (= N262), P244 (≠ S263), N245 (≠ S264), M246 (≠ P265), E247 (≠ G266)
Sites not aligning to the query:
1uc9A Crystal structure of a lysine biosynthesis enzyme, lysx, from thermus thermophilus hb8 (see paper)
28% identity, 78% coverage: 51:285/301 of query aligns to 42:250/256 of 1uc9A
- binding adenosine-5'-diphosphate: V127 (= V139), K129 (= K141), Q159 (= Q177), E160 (= E178), Y161 (= Y179), V162 (≠ I180), K164 (≠ A183), D168 (= D187), R184 (= R203), D213 (= D248), F215 (≠ L250), N224 (≠ M259), E225 (= E260)
7drpA Structure of atp-grasp ligase psnb complexed with phosphomimetic variant of minimal precursor, mg, and adp (see paper)
30% identity, 34% coverage: 185:286/301 of query aligns to 207:306/312 of 7drpA
- binding adenosine-5'-diphosphate: D209 (= D187), D267 (= D248), K269 (≠ L250), L279 (≠ M259), E280 (= E260)
- binding magnesium ion: D267 (= D248), E280 (= E260), E280 (= E260), N282 (= N262)
- binding : R211 (= R189), N282 (= N262), S284 (= S264), A285 (≠ P265), M286 (≠ G266)
Sites not aligning to the query:
- binding adenosine-5'-diphosphate: 131, 146, 168, 170, 178, 202, 203, 204, 205
- binding : 70, 77, 78, 79, 169, 170, 171, 175, 176, 179, 190, 191, 193, 194, 199, 201
7drnA Structure of atp-grasp ligase psnb complexed with precursor peptide psna2 and amppnp (see paper)
30% identity, 34% coverage: 185:286/301 of query aligns to 207:306/311 of 7drnA
Sites not aligning to the query:
- binding phosphoaminophosphonic acid-adenylate ester: 131, 146, 168, 170, 176, 178, 202, 203, 204, 205
- binding : 70, 79, 169, 170, 171, 175, 176, 179, 185, 190, 191, 193, 199
7lg5A Synechocystis sp. Utex2470 cyanophycin synthetase 1 with atp (see paper)
23% identity, 65% coverage: 99:293/301 of query aligns to 219:482/871 of 7lg5A
- binding adenosine-5'-triphosphate: V259 (= V139), H267 (≠ Q147), I271 (≠ V151), I273 (≠ L153), E296 (≠ Q177), R297 (≠ E178), Y298 (= Y179), Y299 (≠ I180), D303 (= D187), T388 (vs. gap), V445 (≠ M259), E446 (= E260)
- binding magnesium ion: D427 (= D248), E446 (= E260)
Sites not aligning to the query:
- binding adenosine-5'-triphosphate: 492, 493, 494, 495, 496, 497, 518, 554, 688, 692, 727, 741, 747, 748, 751
- binding magnesium ion: 496, 518, 554
7droD Structure of atp-grasp ligase psnb complexed with minimal precursor (see paper)
30% identity, 34% coverage: 185:286/301 of query aligns to 187:284/289 of 7droD
Sites not aligning to the query:
3r5xD Crystal structure of d-alanine--d-alanine ligase from bacillus anthracis complexed with atp
26% identity, 44% coverage: 135:267/301 of query aligns to 132:269/306 of 3r5xD
- active site: S144 (≠ Q147), N207 (≠ R211), Y210 (≠ L214), R248 (≠ G246), D250 (= D248), E262 (= E260), N264 (= N262), G268 (= G266)
- binding adenosine-5'-triphosphate: V136 (= V139), K138 (= K141), V148 (= V151), E174 (≠ Q177), K175 (≠ E178), Y176 (= Y179), I177 (= I180), E181 (≠ D187), M252 (≠ L250), M261 (= M259), E262 (= E260), N264 (= N262)
- binding calcium ion: E202 (≠ K206), D205 (≠ E209), Y210 (≠ L214), D250 (= D248), E262 (= E260)
Sites not aligning to the query:
Query Sequence
>Pf6N2E2_3903 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_3903
MKIAVLSRNPRLYSTRRLVEAGTERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDA
VIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFA
HSPDDIPDLIAMVNGAPLVIKVLEGTQGIGVVLCETATAAESVIEAFMGLKQNIMVQEYI
KEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
GLAVAGVDILRSNHGPLVMEVNSSPGLEGIETTTSKNVAGIIIEHLEKNGGPNMTRTKGK
G
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory