SitesBLAST

Comparing Pf6N2E2_4148 Glutamate-aspartate carrier protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
38% identity, 92% coverage: 10:418/443 of query aligns to 10:421/425 of 6zgbA

• binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A277), S273 (= S278), S274 (= S279), M308 (≠ L312), T311 (≠ S315), D392 (= D389), R395 (= R392), T396 (= T393)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
38% identity, 92% coverage: 10:418/443 of query aligns to 9:420/424 of 6zl4A

• binding decyl-beta-d-maltopyranoside: Y190 (≠ F196), L191 (≠ K197), G194 (≠ H200), G195 (≠ M201), Q198 (= Q204), R282 (= R285), E286 (= E291)
• binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A277), S272 (= S278), S273 (= S279), M307 (≠ L312), T310 (≠ S315), V351 (= V356), P352 (= P357), G353 (= G358), A354 (≠ V359), G355 (≠ S360), D391 (= D389), R394 (= R392), T395 (= T393), N398 (= N396)

Sites not aligning to the query:

• binding decyl-beta-d-maltopyranoside: 3

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
38% identity, 92% coverage: 10:418/443 of query aligns to 12:423/427 of 5e9sA

• binding aspartic acid: R274 (≠ A277), S275 (= S278), S276 (= S279), T313 (≠ S315), T351 (≠ I353), A352 (= A354), G353 (= G355), V354 (= V356), P355 (= P357), G356 (= G358), A357 (≠ V359), G358 (≠ S360), D394 (= D389), R397 (= R392), T398 (= T393), N401 (= N396)
• binding decyl-beta-d-maltopyranoside: L194 (≠ K197), G197 (≠ H200), G198 (≠ M201), Y202 (= Y205), R285 (= R285)
• binding sodium ion: Y87 (≠ F88), T90 (≠ V91), S91 (≠ T92), S276 (= S279), G305 (= G307), A306 (≠ Y308), T307 (≠ S309), I308 (≠ F310), N309 (= N311), N309 (= N311), M310 (≠ L312), M310 (≠ L312), D311 (= D313), S348 (= S350), I349 (≠ K351), G350 (= G352), T351 (≠ I353), N401 (= N396), V402 (= V397), D405 (≠ N400)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
38% identity, 92% coverage: 10:418/443 of query aligns to 12:423/426 of 6xwnB

• binding (3s)-3-(benzyloxy)-l-aspartic acid: R274 (≠ A277), S275 (= S278), S276 (= S279), T307 (≠ S309), M310 (≠ L312), T313 (≠ S315), G358 (≠ S360), D394 (= D389), R397 (= R392), T398 (= T393)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
37% identity, 92% coverage: 10:418/443 of query aligns to 5:412/416 of 6r7rA

• binding d-aspartic acid: R263 (≠ A277), S264 (= S278), S265 (= S279), M299 (≠ L312), T302 (≠ S315), T340 (≠ I353), A341 (= A354), G342 (= G355), V343 (= V356), G347 (≠ S360), D383 (= D389), R386 (= R392), T387 (= T393), N390 (= N396)
• binding decyl-beta-d-maltopyranoside: H23 (= H28), V211 (≠ S225), V212 (≠ L226), A216 (= A230), K217 (= K231), G220 (≠ I234)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
36% identity, 91% coverage: 10:412/443 of query aligns to 14:417/425 of O59010

• S65 (= S63) mutation to V: Strongly decreased chloride conductance.
• R276 (≠ A277) mutation to S: Increased rate of aspartate transport; when associated with R-395.
• RSS 276:278 (≠ ASS 277:279) binding
• M311 (≠ L312) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
• T314 (≠ S315) binding
• V355 (= V356) binding
• D394 (= D389) binding
• M395 (= M390) mutation to R: Increased rate of aspartate transport; when associated with S-276.
• R397 (= R392) mutation to A: Strongly decreased affinity for aspartate.
• N401 (= N396) binding
• D405 (≠ N400) mutation to N: Strongly decreased affinity for aspartate.

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
36% identity, 91% coverage: 11:412/443 of query aligns to 15:417/419 of 6x15A

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K15 (≠ Q11), I18 (= I14), Y42 (≠ V40), P45 (= P43), F46 (≠ A44), F46 (≠ A44), F46 (≠ A44), L49 (≠ I47), L49 (≠ I47), L53 (= L51), I73 (≠ V71), P75 (≠ D73), K84 (= K83), L90 (≠ E89), L91 (≠ V90), L91 (≠ V90), F95 (≠ I94), L130 (≠ F138), V131 (≠ I139), I133 (≠ T141), L134 (≠ I142), I137 (≠ L145), L152 (= L160), F156 (= F164), I159 (≠ V167), I163 (≠ L171), I163 (≠ L171), I163 (≠ L171), A164 (≠ G172), Y167 (≠ S175), Y167 (≠ S175), K196 (= K197), I197 (≠ V198), N199 (≠ H200), G200 (≠ M201), Y204 (= Y205), I207 (= I208), F210 (= F211), L250 (≠ I251), I253 (≠ L254), Y254 (≠ F255), Y254 (≠ F255), I262 (≠ M263), K266 (= K267), M269 (≠ L270), T334 (≠ S335), V335 (≠ I336), G336 (≠ S337), Q337 (= Q338), L339 (= L340), T340 (≠ L341), L343 (= L344), S369 (= S370), M399 (≠ A394)
• binding aspartic acid: R276 (≠ A277), S277 (= S278), S278 (= S279), T314 (≠ S315), T352 (≠ I353), G354 (= G355), V355 (= V356), G357 (= G358), A358 (≠ V359), G359 (≠ S360), D394 (= D389), R397 (= R392), T398 (= T393), N401 (= N396)
• binding sodium ion: Y89 (≠ F88), T92 (≠ V91), S93 (≠ T92), G306 (= G307), A307 (≠ Y308), T308 (≠ S309), N310 (= N311), N310 (= N311), M311 (≠ L312), D312 (= D313), S349 (= S350), I350 (≠ K351), G351 (= G352), T352 (≠ I353), N401 (= N396), V402 (= V397), D405 (≠ N400)

Sites not aligning to the query:

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4, 6, 7, 8

2nwwA Crystal structure of gltph in complex with tboa (see paper)
36% identity, 91% coverage: 11:411/443 of query aligns to 6:407/407 of 2nwwA

• binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ A277), S268 (= S278), S269 (= S279), T305 (≠ S315), G348 (= G358), A349 (≠ V359), G350 (≠ S360), D385 (= D389), R388 (= R392), T389 (= T393), N392 (= N396)

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
36% identity, 91% coverage: 11:411/443 of query aligns to 12:413/413 of 6x14A

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G67), A67 (≠ I68), I70 (≠ V71), V83 (≠ I85), I157 (≠ L168), Y164 (≠ S175), K193 (= K197), I194 (≠ V198), N196 (≠ H200), G197 (≠ M201), Q200 (= Q204), Y201 (= Y205), L302 (≠ T306), T305 (≠ S309), I306 (≠ F310), I347 (≠ K351), A350 (= A354)
• binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I12), F47 (= F48), M199 (= M203), R273 (≠ A277), S274 (= S278), S275 (= S279), M308 (≠ L312), T311 (≠ S315), P353 (= P357), G356 (≠ S360), A357 (≠ F361), L384 (≠ A382), D391 (= D389), R394 (= R392), T395 (= T393)

Sites not aligning to the query:

• binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 4

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
35% identity, 91% coverage: 10:412/443 of query aligns to 6:409/409 of 6bavA

• binding benzylcysteine: R268 (≠ A277), S269 (= S278), S270 (= S279), M303 (≠ L312), T306 (≠ S315), Y309 (= Y318), D382 (= D385), D386 (= D389), C389 (≠ R392), T390 (= T393), N393 (= N396)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
35% identity, 91% coverage: 10:411/443 of query aligns to 6:408/408 of 6bauA