SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_4374 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4374 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
78% identity, 99% coverage: 6:465/465 of query aligns to 4:463/463 of P26276

query
sites
P26276

A

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R15 (= R17) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
Y17 (= Y19) binding ; binding
R20 (= R22) mutation to A: No phosphoglucomutase activity.
S108 (= S110) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
N110 (= N112) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
D242 (= D244) binding
D244 (= D246) binding
D246 (= D248) binding
R247 (= R249) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
R262 (= R264) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
K285 (= K287) binding
H308 (= H310) binding ; binding
E325 (= E327) mutation to A: Reduces KM and Vmax approximately 2-fold.
EMSGH 325:329 (= EMSGH 327:331) binding ; binding
H329 (= H331) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
P368 (= P370) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
R421 (= R423) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
RASNT 421:425 (= RASNT 423:427) binding ; binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
78% identity, 99% coverage: 6:465/465 of query aligns to 4:463/463 of Q02E40

query
sites
Q02E40

A

A

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H

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S108 (= S110) active site, Non-phosphorylated intermediate; modified: Phosphoserine

1pcjX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
78% identity, 98% coverage: 8:465/465 of query aligns to 1:458/458 of 1pcjX

query
sites
1pcjX

A

A

P

P

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L

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H

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K

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A

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A

A

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D

D

C

C

G

G

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G

A

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A

A

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A

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C

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V

D

D

G

G

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F

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P

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N

H

H

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D

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P

G

G

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E

E

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N

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D

D

L

L

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I

A

A

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K

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V

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K

E

A

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E

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N

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A

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D

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L

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G

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A

A

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D

G

G

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D

G

G

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D

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R

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G

G

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T

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R

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M

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L

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F

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A

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K

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D

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R

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M

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H

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F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: R15 (= R22), S103 (= S110), H104 (= H111), K113 (= K120), D237 (= D244), D239 (= D246), D241 (= D248), R242 (= R249), H324 (= H331), D335 (= D342)
binding 1-O-phosphono-alpha-D-mannopyranose: Y12 (= Y19), R15 (= R22), S103 (= S110), T301 (= T308), G302 (= G309), H303 (= H310), E320 (= E327), S322 (= S329), H324 (= H331), R416 (= R423), S418 (= S425), N419 (= N426), T420 (= T427)
binding zinc ion: S103 (= S110), D237 (= D244), D239 (= D246), D241 (= D248)

1k2yX Crystal structure of phosphomannomutase/phosphoglucomutase s108a mutant from p. Aeruginosa (see paper)
78% identity, 98% coverage: 8:465/465 of query aligns to 2:459/459 of 1k2yX

query
sites
1k2yX

A

A

P

P

I

T

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y

Y

D

D

I

I

R

R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S

A

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K

K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H

H

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: H105 (= H111), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), D336 (= D342)
binding zinc ion: D238 (= D244), D240 (= D246), D242 (= D248)

4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
78% identity, 98% coverage: 8:465/465 of query aligns to 2:459/459 of 4il8A

query
sites
4il8A

A

A

P

P

I

T

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y

Y

D

D

I

I

R
|
R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K

K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E

E

M

M

S

S

G

G

H
x
A

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R

R

A

A

S

S

N

N

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: R16 (= R22), S104 (= S110), H105 (= H111), K114 (= K120), D238 (= D244), D240 (= D246), D242 (= D248), R243 (= R249), A325 (≠ H331), D336 (= D342)
binding magnesium ion: S104 (= S110), D238 (= D244), D240 (= D246), D242 (= D248)

2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
78% identity, 98% coverage: 11:465/465 of query aligns to 1:455/455 of 2h5aX

query
sites
2h5aX

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R

R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K

K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: H101 (= H111), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), D332 (= D342)
binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (= Y19), T298 (= T308), G299 (= G309), H300 (= H310), E317 (= E327), S319 (= S329), H321 (= H331), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S110), D234 (= D244), D236 (= D246), D238 (= D248)

2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
78% identity, 98% coverage: 11:465/465 of query aligns to 1:455/455 of 2h4lX

query
sites
2h4lX

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K

K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K
|
K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T
|
T

G
|
G

H

H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H

H

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: H101 (= H111), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), D332 (= D342)
binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (= Y19), R12 (= R22), S100 (= S110), K277 (= K287), T298 (= T308), G299 (= G309), E317 (= E327), S319 (= S329), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S110), D234 (= D244), D236 (= D246), D238 (= D248)

2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
78% identity, 98% coverage: 11:465/465 of query aligns to 1:455/455 of 2fkfA

query
sites
2fkfA

F

L

P

P

D

A

S

S

I

I

F

F

R
|
R

A

A

Y

Y

D

D

I

I

R
|
R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N
|
N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K

K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T

T

G

G

H

H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E

E

M

M

S
|
S

G

G

H
|
H

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: R12 (= R22), S100 (= S110), H101 (= H111), K110 (= K120), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (= R17), R12 (= R22), H101 (= H111), N102 (= N112), S319 (= S329), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S110), D234 (= D244), D236 (= D246), D238 (= D248)

1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
78% identity, 98% coverage: 11:465/465 of query aligns to 1:455/455 of 1pcmX

query
sites
1pcmX

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K
|
K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M

W

W

K

K

T
|
T

G
|
G

H
|
H

S

S

L

L

I

I

K

K

M

M

K

K

Q

E

S

T

G

G

A

A

L

L

A

A

G

G

E
|
E

M

M

S
|
S

G

G

H
|
H

I

V

F

F

K

K

E

E

R

R

W

W

F

F

G

G

F

F

D
|
D

D

D

G

G

I

I

Y

Y

S

S

A

A

R

R

L

L

E

E

I

I

L

L

S

S

K

Q

E

D

K

Q

S

R

T

D

A

S

E

E

E

H

L

V

F

F

A

S

T

A

F

F

P

P

N

S

D

D

I

I

S

S

T

T

P

P

E

E

I

I

N

N

I

I

H

T

V

V

T

T

E

E

E

D

S

S

K

K

F

F

S

A

I

I

D

E

A

A

L

L

H

Q

-

R

D

D

A

A

Q

Q

W

W

G

G

E

E

G

G

A

-

E

N

L

I

T

T

I

L

D

D

G

G

V

V

R

R

V

V

D

D

Y

Y

P

P

H

K

G

G

W

W

G

G

L

L

V

V

R
|
R

A

A

S
|
S

N
|
N

T
|
T

T

T

P

P

V

V

L

L

V

V

L

L

R

R

F

F

E

E

A

A

D

D

N

T

E

E

A

E

E

E

L

L

Q

E

R

R

I

I

K

K

D

T

V

V

F

F

H

R

T

N

Q

Q

L

L

K

K

R

A

V

V

A

D

P

S

D

S

L

L

Q

P

L

V

P

P

F

F

active site: R12 (= R22), S100 (= S110), H101 (= H111), K110 (= K120), D234 (= D244), D236 (= D246), D238 (= D248), R239 (= R249), H321 (= H331), D332 (= D342)
binding 6-O-phosphono-alpha-D-mannopyranose: Y9 (= Y19), S100 (= S110), K277 (= K287), T298 (= T308), G299 (= G309), H300 (= H310), E317 (= E327), S319 (= S329), H321 (= H331), R413 (= R423), S415 (= S425), N416 (= N426), T417 (= T427)
binding zinc ion: S100 (= S110), D234 (= D244), D236 (= D246), D238 (= D248)

1p5gX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
78% identity, 98% coverage: 11:465/465 of query aligns to 1:455/455 of 1p5gX

query
sites
1p5gX

F

L

P

P

D

A

S

S

I

I

F

F

R

R

A

A

Y
|
Y

D

D

I

I

R
|
R

G

G

V

V

P

G

E

D

T

T

L

L

T

T

A

A

E

E

T

T

A

A

Y

Y

W

W

I

I

G

G

R

R

A

A

I

I

G

G

S

S

Q

E

S

S

L

L

A

A

Q

R

G

G

E

E

P

P

N

C

V

V

C

A

V

V

G

G

R

R

D

D

G

G

R

R

L

L

S

S

G

G

P

P

E

E

L

L

V

V

E

K

Q

Q

L

L

I

I

K

Q

G

G

V

L

A

V

D

D

S

C

G

G

C

C

H

Q

V

V

S

S

D

D

V

V

G

G

L

M

V

V

P

P

T

T

P

P

A

V

L

L

Y

Y

A

A

N

N

V

V

L

L

A

E

G

G

K

K

S

S

G

G

V

V

M

M

L

L

T

T

G

G

S
|
S

H
|
H

N

N

P

P

S

P

N

D

Y

Y

N

N

G

G

F

F

K
|
K

I

I

V

V

I

V

A

A

G

G

D

E

T

T

L

L

A

A

N

N

E

E

Q

Q

I

I

Q

Q

A

A

L

L

H

R

T

E

R

R

L

I

K

E

T

K

N

N

D

D

L

L

S

A

S

S

G

G

Q

V

G

G

S

S

I

V

T

E

K

Q

V

V

D

D

I

I

L

L

E

P

R

R

Y

Y

N

F

D

K

E

Q

I

I

V

R

K

D

D

D

V

I

K

A

L

M

A

A

R

K

R

P

L

M

K

K

V

V

D

D

C

C

G

G

N

N

G

G

A

V

A

A

G

G

V

V

I

I

A

A

P

P

Q

Q

L

L

I

I

E

E

A

A

L

L

N

G

C

C

E

S

V

V

I

I

P

P

L

L

F

Y

C

C

D

E

V

V

D

D

G

G

N

N

F

F

P

P

N

N

H

H

P

P

D

D

P

P

G

G

K

K

L

P

E

E

N

N

L

L

E

K

D

D

L

L

I

I

A

A

K

K

V

V

K

K

E

A

T

E

N

N

A

A

D

D

L

L

G

G

L

L

A

A

F

F

D
|
D

G

G

D
|
D

G

G

D
|
D

R
|
R

V

V

G

G

V

V

T

T

N

N

T

T

G

G

S

T

V

I

F

Y

P

P

D

D

R

R

L

L

M

M

L

L

F

F

A

A

K

K

D

D

V

V

A

S

R

R

N

N

P

P

N

G

A

A

E

D

I

I

F

F

D

D

V

V

K
|
K

C

C

T

T

R

R

L

L

V

I

P

A

L

L

I

I

K

S

E

G

Y

Y

G

G

R

R

P

P

L

V

M

M