SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_455 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_455 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

D7A5Q8 Aromatic amino acid exporter YddG from Starkeya novella (strain ATCC 8093 / DSM 506 / JCM 20403 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIMB 10456) (see paper)
28% identity, 61% coverage: 84:275/314 of query aligns to 71:254/287 of D7A5Q8

query
sites
D7A5Q8

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Y78 (= Y91) mutation to A: Shows moderate effects on the transport activities of both threonine and methionine.
H79 (≠ N92) mutation to A: Abolishes the transport activities for both threonine and methionine.
Y82 (≠ F95) mutation to A: Enhances methionine transport and slightly reduces threonine transport.
W101 (≠ N114) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.
W163 (= W177) mutation to A: Exhibits decreased transport activity for threonine, but not for methionine.

Query Sequence

>Pf6N2E2_455 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_455
MKTLEQTLPTPSDLPVYLKLAMVTMIWGGTFVAGRILSDALAPMFAASLRFLLASIALLA
FLALAGIPLARPSVKQGLHLAALGFFGIFFYNLCFFYGLQYINASRASLIVALNPAVIGL
ASWWLFKERLSRLKVAGIVLCIGGAGLVIVSRDPSLLQGAAQGWIGDLLIFGCVLGWGIY
SLFSRRLNDSLGPLQTVTWSILLGTLMLWLACAAAGEIRVEALRALDLEQWLSLLYLGVL
GSALAYIAWYDGIRRIGATRSGVFIALNPLTAVLLGALLLDERLTALMCVGGGVILVGIF
LCNKPLARSKEKAI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory