SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_4682 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4682 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
27% identity, 62% coverage: 98:270/281 of query aligns to 64:236/553 of B5Z7I3

query
sites
B5Z7I3

V

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A

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G

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A

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N

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M

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D

N

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Q

Y

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T

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-

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L

F

F

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Y

L

L

F

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I

P

P

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L

I

T

-

L

-

F

-

G

G

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V

G

G

V

L

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K

P

N

G

A

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L

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L

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L

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Y

A

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A

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P

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Y

Y

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E

E

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G

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C

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E

L

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R

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H

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H

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G

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G

G

G

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L

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A

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Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>Pf6N2E2_4682 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4682
MLIDQKIPLGQYIAGFVEWLTQNGANTFDAIALFLETMIHGVTFALTWFNPLALIGLIAL
LAHFIQRKWGLTIFVIASFLLILNLGYWQETMETLAQVLFATLVCVLIGVPLGIVAAHKP
MFYTMMRPVLDLMQTVPTFVYLIPTLTLFGLGVVPGLISTVVFAIAAPIRLTYLGIRDVP
DELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKVGGDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory