SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_4682 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4682 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

A0A0H2ZQB9 Ergothioneine transporter EgtUBC from Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) (see paper)
31% identity, 50% coverage: 132:271/281 of query aligns to 60:199/506 of A0A0H2ZQB9

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A0A0H2ZQB9

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Sites not aligning to the query:

244 G→F: Disrupts ergothioneine (EGT) binding. Binds EGT 100-fold more weakly. May have a global impact on overall structure.
293 F→Y: Disrupts ergothioneine (EGT) binding.
375 E→Q: Disrupts ergothioneine (EGT) binding. Binds EGT 500-fold more weakly.
419 Y→F: Disrupts ergothioneine (EGT) binding.

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
27% identity, 62% coverage: 98:270/281 of query aligns to 64:236/553 of B5Z7I3

query
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B5Z7I3

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Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

8wm8B Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with nitrate (see paper)
28% identity, 58% coverage: 119:280/281 of query aligns to 99:264/265 of 8wm8B

query
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8wm8B

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binding nitrate ion: L203 (= L222), A207 (≠ V226)

Query Sequence

>Pf6N2E2_4682 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4682
MLIDQKIPLGQYIAGFVEWLTQNGANTFDAIALFLETMIHGVTFALTWFNPLALIGLIAL
LAHFIQRKWGLTIFVIASFLLILNLGYWQETMETLAQVLFATLVCVLIGVPLGIVAAHKP
MFYTMMRPVLDLMQTVPTFVYLIPTLTLFGLGVVPGLISTVVFAIAAPIRLTYLGIRDVP
DELMDAGKAFGCSRRQLLSRIELPHAMPSIAAGITQCIMLSLSMVVIAALVGADGLGKPV
VNALNTADIALGFEAGLAIVLLAIMLDRICKQPDAKVGGDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory