SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_4818 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4818 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

8hpsB ABC transporter, permease protein SugB (see paper)
28% identity, 88% coverage: 18:255/269 of query aligns to 14:259/264 of 8hpsB

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8hpsB

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binding alpha-D-glucopyranose: H113 (≠ V114), F200 (≠ T201)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
27% identity, 87% coverage: 23:257/269 of query aligns to 27:285/296 of P68183

query
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P68183

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E190 (≠ R166) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A168) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G172) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P185) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
28% identity, 45% coverage: 98:217/269 of query aligns to 313:454/514 of P02916

query
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P02916

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L334 (vs. gap) mutation to W: Ability to transport lactose in a saturable manner.
L372 (= L137) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ H141) mutation N->K,H: No growth on maltose.
G380 (= G145) mutation G->C,S: No growth on maltose.
E401 (≠ R166) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A168) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G172) mutation G->A,P: No effect.
P420 (= P185) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
28% identity, 45% coverage: 98:217/269 of query aligns to 298:439/490 of 4ki0F

query
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4ki0F

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binding alpha-D-glucopyranose: Y310 (≠ M110), N361 (≠ H141), L364 (= L144), G365 (= G145), Y368 (≠ F148), A417 (≠ F197), S418 (≠ A198), F421 (≠ T201), F421 (≠ T201), F421 (≠ T201), N422 (≠ S202), N425 (≠ E205)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 276

Query Sequence

>Pf6N2E2_4818 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4818
MSPVERVWFYCLRTLCGLILLFLILPVLVIIPLSFNSGSFLVYPLQGFSLQWYHDFFASA
EWMRALKNSIIVAPAATLLAMVFGTLAAIGLTRGDFPGKALVMALVISPMVVPVVIIGVA
SYLFFAPLGMGNSFFSLIVVHAVLGVPFVIITVSATLQGFNHNLVRAAASLGASPLTAFR
RVTLPLIAPGVISGALFAFATSFDEVVVTLFLAGPEQATLPRQMFSGIRENLSPTIAAAA
TLLIAFSVILLLTLEWLRGRSEKLRTTQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory