SitesBLAST
Comparing Pf6N2E2_4825 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4825 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
57% identity, 98% coverage: 2:344/349 of query aligns to 3:330/331 of 4yi7A
1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
37% identity, 96% coverage: 1:336/349 of query aligns to 1:326/344 of 1zxyA
- binding magnesium ion: D223 (= D227), E224 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V81), G79 (= G82), D83 (= D86), T87 (≠ I90), N89 (= N92), S91 (= S94), T92 (= T95), K106 (= K110), S118 (= S122), D223 (= D227), E224 (= E228)
2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
37% identity, 96% coverage: 1:336/349 of query aligns to 1:326/345 of 2gvqD
P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
37% identity, 96% coverage: 1:336/349 of query aligns to 1:326/345 of P50384
- T87 (≠ I90) binding 5-phospho-alpha-D-ribose 1-diphosphate
- K106 (= K110) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
- H107 (= H111) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
- S118 (= S122) binding 5-phospho-alpha-D-ribose 1-diphosphate
- H154 (= H158) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
- R164 (= R168) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
- D223 (= D227) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
- E224 (= E228) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
38% identity, 95% coverage: 7:338/349 of query aligns to 6:329/330 of 1kgzB
- binding manganese (ii) ion: S92 (= S94), D222 (= D227), E223 (= E228), E223 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (= V81), G82 (= G84), G83 (= G85), N90 (= N92), I91 (≠ V93), S92 (= S94), T93 (= T95), K108 (= K110), S118 (= S122)
1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
36% identity, 96% coverage: 1:336/349 of query aligns to 1:322/339 of 1gxbA
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
38% identity, 96% coverage: 7:340/349 of query aligns to 4:323/325 of 5nofA
3gbrA Anthranilate phosphoribosyl-transferase (trpd) double mutant d83g f149s from s. Solfataricus (see paper)
36% identity, 96% coverage: 1:336/349 of query aligns to 1:323/341 of 3gbrA
- binding manganese (ii) ion: S91 (= S94), D220 (= D227), E221 (= E228), E221 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ V81), G79 (= G82), T80 (= T83), G81 (= G84), N89 (= N92), V90 (= V93), S91 (= S94), T92 (= T95), K106 (= K110), G114 (= G121)
4n93A Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
38% identity, 95% coverage: 7:339/349 of query aligns to 8:342/346 of 4n93A
- active site: V83 (= V81)
- binding 2-amino-6-methylbenzoic acid: V83 (= V81), G84 (= G82), T85 (= T83), H113 (= H111), N115 (= N113), N115 (= N113), A156 (= A154), P157 (≠ Q155), H160 (= H158), Y163 (≠ M161), A167 (= A165), R170 (= R168), G183 (= G181), P184 (= P182)
- binding magnesium ion: S96 (= S94), D228 (= D227), E229 (= E228), E229 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G82), G86 (= G84), G87 (= G85), N94 (= N92), S96 (= S94), T97 (= T95), K112 (= K110), A117 (= A115), A118 (≠ V116), S120 (≠ G118), G123 (= G121)
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
38% identity, 95% coverage: 7:339/349 of query aligns to 7:340/344 of 3r88B
- active site: V82 (= V81)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N113), A155 (= A154), P156 (≠ Q155), H159 (= H158), Y162 (≠ M161), R163 (≠ K162), A166 (= A165)
- binding magnesium ion: S95 (= S94), D227 (= D227), E228 (= E228), E228 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (= V81), G83 (= G82), G85 (= G84), G86 (= G85), N93 (= N92), S95 (= S94), T96 (= T95), K111 (= K110), N114 (= N113), A117 (≠ V116), S118 (= S117), S119 (≠ G118)
5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
38% identity, 95% coverage: 7:339/349 of query aligns to 7:340/343 of 5c1rA
- active site: V82 (= V81)
- binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (= V81), G83 (= G82), G86 (= G85), N93 (= N92), S95 (= S94), T96 (= T95), K111 (= K110), R115 (= R114), A117 (≠ V116), S118 (= S117), S119 (≠ G118)
- binding magnesium ion: D87 (= D86), V89 (≠ A88), S95 (= S94), E228 (= E228)
4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
38% identity, 95% coverage: 7:339/349 of query aligns to 9:343/347 of 4n5vA
- active site: V84 (= V81)
- binding 2-amino-4-fluorobenzoic acid: V84 (= V81), G85 (= G82), H114 (= H111), G115 (= G112), N116 (= N113), A157 (= A154), A157 (= A154), P158 (≠ Q155), H161 (= H158), Y164 (≠ M161), R165 (≠ K162), A168 (= A165), R171 (= R168), G184 (= G181)
- binding magnesium ion: S97 (= S94), D229 (= D227), E230 (= E228), E230 (= E228)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G82), G87 (= G84), G88 (= G85), N95 (= N92), S97 (= S94), T98 (= T95), K113 (= K110), A119 (≠ V116), S120 (= S117), S121 (≠ G118), G124 (= G121)
4owoA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 6-fluoroanthranilate, prpp and magnesium (see paper)
38% identity, 95% coverage: 7:339/349 of query aligns to 9:344/348 of 4owoA