SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_489 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_489 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7CRQ0 Uronate dehydrogenase; D-galacturonate dehydrogenase; D-glucuronate dehydrogenase; Hexuronate dehydrogenase; EC 1.1.1.203 from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see paper)
53% identity, 93% coverage: 12:266/273 of query aligns to 3:257/265 of Q7CRQ0

query
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Q7CRQ0

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Y134 (= Y144) mutation to A: 0.1% of wild-type activity.

3rfvA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens complexed with nadh and product (see paper)
52% identity, 94% coverage: 10:266/273 of query aligns to 2:258/265 of 3rfvA

query
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3rfvA

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binding D-galactaro-1,5-lactone: S74 (= S83), S110 (= S119), N111 (= N120), H112 (= H121), Y135 (= Y144), S164 (= S173), R173 (= R182), F257 (= F265)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G17), G12 (= G20), Q13 (≠ G21), L14 (= L22), D33 (= D41), L34 (≠ I42), D50 (= D59), L51 (= L60), L70 (≠ F79), G71 (= G80), G72 (= G81), S74 (= S83), A108 (= A117), S110 (= S119), Y135 (= Y144), K139 (= K148), I162 (= I171), S164 (= S173), C165 (≠ S174)

3rfxA Crystal structure of uronate dehydrogenase from agrobacterium tumefaciens, y136a mutant complexed with NAD (see paper)
52% identity, 94% coverage: 10:266/273 of query aligns to 2:258/265 of 3rfxA

query
sites
3rfxA

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binding nicotinamide-adenine-dinucleotide: G9 (= G17), G12 (= G20), Q13 (≠ G21), L14 (= L22), D33 (= D41), L34 (≠ I42), D50 (= D59), L51 (= L60), G72 (= G81), S74 (= S83)

4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
31% identity, 58% coverage: 13:170/273 of query aligns to 2:158/321 of 4id9A

query
sites
4id9A

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active site: S106 (= S119), Y132 (= Y144), K136 (= K148)
binding nicotinamide-adenine-dinucleotide: G9 (= G20), R10 (≠ G21), V11 (≠ L22), D30 (= D41), L31 (≠ M45), R32 (≠ A46), S44 (≠ D59), L45 (= L60), L64 (≠ F79), A66 (≠ G81), V81 (≠ A94), S106 (= S119), Y132 (= Y144), K136 (= K148)

Sites not aligning to the query:

4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
31% identity, 58% coverage: 13:171/273 of query aligns to 3:160/328 of 4id9B

query
sites
4id9B

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active site: S107 (= S119), Y133 (= Y144), K137 (= K148)
binding nicotinamide-adenine-dinucleotide: G10 (= G20), R11 (≠ G21), V12 (≠ L22), D31 (= D41), L32 (≠ M45), S45 (≠ D59), L46 (= L60), L65 (≠ F79), A67 (≠ G81), V82 (≠ A94), Y133 (= Y144), K137 (= K148), F160 (≠ I171)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 161, 162, 163

3a4vA Crystal structure of pyruvate bound l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
26% identity, 58% coverage: 13:170/273 of query aligns to 2:163/311 of 3a4vA

query
sites
3a4vA

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R

Q

D

A

E

V

I

H

D

Q

R

L

A

V

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

I

I

L

F

H

H

F
x
L

G
x
A

G
|
G

V

I

-
x
L

-
x
S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

G

K

A

V

N

N

I

M

S

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

K

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A
x
P

S
|
S

S
x
T

N

I

H

G

V

V

I

F

G

G

F

P

Y

E

K

T

Q

P

D

K

E

N

T

K

I

V

D

P

A

S

L

I

S

T

P

I

R

T

R

R

P

P

D

R

G

T

Y

M

Y
|
Y

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

V

L

A

G

S

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

I

L

E

D

T

V

V

R

S

S

I

L

R

R

active site: T112 (≠ S119), Y137 (= Y144), K141 (= K148)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A19), G9 (= G20), Q10 (≠ G21), I11 (≠ L22), D32 (= D41), I33 (= I42), L46 (≠ C58), D47 (= D59), V48 (≠ L60), L69 (≠ F79), A70 (≠ G80), G71 (= G81), L73 (vs. gap), P110 (≠ A117), S111 (= S118), T112 (≠ S119), Y137 (= Y144), K141 (= K148)
binding pyruvic acid: S74 (vs. gap), Y137 (= Y144)

Sites not aligning to the query:

3a1nA Crystal structure of l-threonine dehydrogenase from hyperthermophilic archaeon thermoplasma volcanium (see paper)
26% identity, 58% coverage: 13:170/273 of query aligns to 2:163/315 of 3a1nA

query
sites
3a1nA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

G
x
Q

L
x
I

G

G

K

T

V

E

L

L

R

V

E

P

R

Y

L

L

-

A

-

E

K

K

P

Y

F

G

A

K

R

K

H

N

I

V

R

I

L

A

S

S

D
|
D

I
|
I

A

V

N

Q

M

R

A

D

P

T

A

G

I

-

D

-

E

-

S

G

E

I

E

K

V

F

M

I

P

T

C
x
L

D
|
D

L

V

S

S

D

N

K

R

Q

D

A

E

V

I

H

D

Q

R

L

A

V

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

I

I

L

F

H

H

F
x
L

G
x
A

G
|
G

V

I

-
x
L

-

S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

G

K

A

V

N

N

I

M

S

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

K

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A

P

S
|
S

S
x
T

N

I

H

G

V

V

I

F

G

G

F

P

Y

E

K

T

Q

P

D

K

E

N

T

K

I

V

D

P

A

S

L

I

S

T

P

I

R

T

R

R

P

P

D

R

G

T

Y

M

Y
|
Y

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

V

L

A

G

S

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

I

L

E

D

T

V

V

R

S

S

I

L

R

R

active site: T112 (≠ S119), Y137 (= Y144), K141 (= K148)
binding nicotinamide-adenine-dinucleotide: S8 (≠ A19), G9 (= G20), Q10 (≠ G21), I11 (≠ L22), D32 (= D41), I33 (= I42), L46 (≠ C58), D47 (= D59), L69 (≠ F79), A70 (≠ G80), G71 (= G81), L73 (vs. gap), S111 (= S118), Y137 (= Y144), K141 (= K148)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 164

3ajrA Crystal structure of l-3-hydroxynorvaline bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
25% identity, 58% coverage: 13:170/273 of query aligns to 2:163/314 of 3ajrA

query
sites
3ajrA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

G
x
Q

L
x
I

G

G

K

T

V

E

L

L

R

V

E

P

R

Y

L

L

-

A

-

E

K

K

P

Y

F

G

A

K

R

K

H

N

I

V

R

I

L

A

S

S

D
|
D

I
|
I

A

V

N

Q

M

R

A

D

P

T

A

G

I

-

D

-

E

-

S

G

E

I

E

K

V

F

M

I

P

T

C
x
L

D
|
D

L
x
V

S

S

D

N

K

R

Q

D

A

E

V

I

H

D

Q

R

L

A

V

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

I

I

L

F

H

H

F
x
L

G
x
A

G
|
G

V

I

-
x
L

-
x
S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

G

K

A

V

N

N

I

M

S

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

K

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A
x
P

S
|
S

S
x
T

N
x
I

H

G

V

V

I

F

G

G

F

P

Y

E

K

T

Q

P

D

K

E

N

T

K

I

V

D

P

A

S

L

I

S

T

P

I

R

T

R

R

P

P

D

R

G

T

Y

M

Y
x
F

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

V

L

A

G

S

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

I

L

E

D

T

V

V

R

S

S

I

L

R

R

binding nicotinamide-adenine-dinucleotide: S8 (≠ A19), G9 (= G20), Q10 (≠ G21), I11 (≠ L22), D32 (= D41), I33 (= I42), L46 (≠ C58), D47 (= D59), V48 (≠ L60), L69 (≠ F79), A70 (≠ G80), G71 (= G81), L73 (vs. gap), P110 (≠ A117), S111 (= S118), T112 (≠ S119), F137 (≠ Y144), K141 (= K148)
binding (3R)-3-hydroxy-L-norvaline: S74 (vs. gap), T112 (≠ S119), I113 (≠ N120), F137 (≠ Y144)

Sites not aligning to the query:

3a9wA Crystal structure of l-threonine bound l-threonine dehydrogenase (y137f) from hyperthermophilic archaeon thermoplasma volcanium (see paper)
25% identity, 58% coverage: 13:170/273 of query aligns to 2:163/315 of 3a9wA

query
sites
3a9wA

L

I

L

L

L

V

T

T

G

G

A

S

A
x
S

G
|
G

G
x
Q

L
x
I

G

G

K

T

V

E

L

L

R

V

E

P

R

Y

L

L

-

A

-

E

K

K

P

Y

F

G

A

K

R

K

H

N

I

V

R

I

L

A

S

S

D
|
D

I
|
I

A

V

N

Q

M

R

A

D

P

T

A

G

I

-

D

-

E

-

S

G

E

I

E

K

V

F

M

I

P

T

C
x
L

D
|
D

L
x
V

S

S

D

N

K

R

Q

D

A

E

V

I

H

D

Q

R

L

A

V

V

E

E

-

K

-

Y

G

S

V

I

D

D

A

A

I

I

L

F

H

H

F
x
L

G
x
A

G

G

V

I

-
x
L

-
x
S

-

A

S

K

V

G

E

E

R

K

P

D

F

P

E

A

E

L

I

A

L

Y

G

K

A
x
V

N

N

I

M

S

N

G

G

V

T

F

Y

H

N

I

I

Y

L

E

E

A

A

R

K

K

Q

H

H

G

R

V

V

K

E

R

K

V

V

I

V

F

I

A
x
P

S
|
S

S
x
T

N

I

H

G

V

V

I

F

G

G

F

P

Y

E

K

T

Q

P

D

K

E

N

T

K

I

V

D

P

A

S

L

I

S

T

P

I

R

T

R

R

P

P

D

R

G

T

Y

M

Y
x
F

G

G

L

V

S

T

K
|
K

S

I

Y

A

G

A

E

E

D

L

V

L

A

G

S

Q

F

Y

Y

Y

F

Y

D

E

R

K

Y

F

G

G

I

L

E

D

T

V

V

R

S

S

I

L

R

R

binding nicotinamide-adenine-dinucleotide: S8 (≠ A19), G9 (= G20), Q10 (≠ G21), I11 (≠ L22), D32 (= D41), I33 (= I42), L46 (≠ C58), D47 (= D59), V48 (≠ L60), L69 (≠ F79), A70 (≠ G80), L73 (vs. gap), V87 (≠ A94), P110 (≠ A117), S111 (= S118), K141 (= K148)
binding threonine: S74 (vs. gap), T112 (≠ S119), F137 (≠ Y144)

Sites not aligning to the query:

6jygD Crystal structure of l-threonine dehydrogenase from phytophthora infestans (see paper)
30% identity, 59% coverage: 11:170/273 of query aligns to 1:162/307 of 6jygD

query
sites
6jygD

N

S

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A
x
T

G
|
G

G
x
Q

L
x
I

G

G

K

M

V

-

L

-

R

-

E

-

R

E

L

L

K

V

P

P

F

Y

A

M

R

R

H

Q

I

L

R

F

L

G

S

A

D

D

-

T

I

I

A

V

N

N

-

S

-
x
D

-
x
I

M
x
K

A

A

P

P

A

G

I

R

D

D

E

G

S

N

E

-

V

F

M

V

P

Y

C

C

D
|
D

L
x
V

S

Q

D

D

K

R

Q

D

A

S

V

M

H

A

Q

R

L

I

V

V

-

T

-

E

E

Q

G

G

V

V

D

D

A

T

I

I

L

V

H

H

F
x
M

G
x
A

G
x
S

V

L

S
x
L

V
x
S

-

A

-

V

-

G

E

E

R

A

P

N

F

P

E

S

E

L

I

A

L

L

G

S

A
x
V

N

N

I

T

S

R

G

G

V

I

F

Q

H

N

I

V

Y

L

E

E

A

L

A

A

R

K

K

Q

H

Y

G

Q

V

L

K

-

R

R

V

V

I

F

F

A

A
x
P

S
|
S

S
x
T

N

I

H

A

V

V

I

F

G

G

-

P

F

S

Y

T

K

P

Q

Q

D

D

E

E

T

T

I

P

D

D

A

T

L

-

S

T

P

I

R

M

R

R

P

P

D

T

G

T

Y

M

Y
|
Y

G

G

L

L

S

T

K
|
K

S

V

Y

H

G

V

E

E

D

L

V

L

A

G

S

E

F

Y

Y

Y

F

Y

D

N

R

K

Y

F

G

G

I

V

E

D

T

F

V

R

S

S

I

V

R

R

binding citrate anion: Q11 (≠ G21), L73 (≠ S83), S74 (≠ V84), Y136 (= Y144)
binding nicotinamide-adenine-dinucleotide: G7 (= G17), T9 (≠ A19), G10 (= G20), Q11 (≠ G21), I12 (≠ L22), D33 (vs. gap), I34 (vs. gap), K35 (≠ M45), D47 (= D59), V48 (≠ L60), M69 (≠ F79), A70 (≠ G80), S71 (≠ G81), L73 (≠ S83), V87 (≠ A94), P109 (≠ A117), S110 (= S118), T111 (≠ S119), Y136 (= Y144), K140 (= K148)

Sites not aligning to the query:

7cgvA Full consensus l-threonine 3-dehydrogenase, fctdh-iiym (NAD+ bound form) (see paper)
28% identity, 59% coverage: 11:170/273 of query aligns to 1:165/310 of 7cgvA

query
sites
7cgvA

N

K

R

R

L

I

L

L

L

V

T

I

G
|
G

A

A

A

N

G
|
G

G
x
Q

L
x
I

G

G

K

S

V

E

L

L

R

V

E

P

R

A

L

L

K

R

P

K

F

R

-

Y

-

G

A

A

R

D

H

N

I

V

R

I

L

A

S

S

D
|
D

I
|
I

A

-

N

-

M
x
R

A

P

P

P

A

N

I

L

D

Y

E

E

S

A

E

G

E

P

V

F

M

E

P

Y

C
x
L

D
|
D

L
x
V

S

L

D

D

K

K

Q

E

A

A

V

L

H

A

Q

E

L

L

V

V

E

K

G

K

-

Y

-

K

V

I

D

T

A

Q

I

I

L

Y

H

H

F

L

G
x
A

V

L

-
x
L

-

S

-

A

S

T

V

G

E

E

R

K

P

N

F

P

E

Q

E

K

I

A

L

W

G

D

A

L

N

N

I

M

S

D

G

G

V

L

F

L

H

N

I

V

Y

L

E

E

A

I

A

A

R

R

K

E

H

R

G

G

V

P

K

L

R

K

V

V

I

F

F

W

A
x
P

S

S

S
|
S

N

I

H

A

V

A

I

F

G

G

F

P

Y

N

K

T

Q

P

D

K

E

D

T

N

I

T

D

P

A

Q

L

D

S

T

P

I

R

M

R

R

P

P

D

T

G

T

Y

I

Y
|
Y

G

G

L

I

S

S

K
|
K

S

V

Y

A

G

G

E

E

D

L

V

L

A

C

S

E

F

Y

Y

Y

F

H

D

T

R

K

Y

Y

G

G

I

V

E

D

T

V

V

R

S

S

I

V

R

R

binding nicotinamide-adenine-dinucleotide: G7 (= G17), G10 (= G20), Q11 (≠ G21), I12 (≠ L22), D33 (= D41), I34 (= I42), R35 (≠ M45), L48 (≠ C58), D49 (= D59), V50 (≠ L60), A72 (≠ G80), A73 (≠ G81), L75 (vs. gap), P112 (≠ A117), S114 (= S119), Y139 (= Y144), K143 (= K148)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 166, 169

3aw9A Structure of udp-galactose 4-epimerase mutant
29% identity, 58% coverage: 12:170/273 of query aligns to 2:157/304 of 3aw9A

query
sites
3aw9A

R

R

L

I

L

V

T

T

G
|
G

A

G

A

A

G
|
G

G
x
F

L
x
I

G

G

K

S

V

H

L

L

R

V

E

D

R

K

L

L

K

V

P

E

F

L

A

G

R

Y

H

E

I

V

R

V

L

V

S

V

D
|
D

I
|
I

A

V

N

Q

M

R

A

D

P

T

A

G

I

-

D

-

E

G

S

S

E

A

E

E

V

L

M

H

P

V

C
x
R

D
|
D

L
|
L

S

K

D

D

K

-

Q

-

A

-

V

-

H

Y

Q

S

L

W

V

G

E

A

G

G

V

I

-

K

-

G

D

D

A

V

I

V

L

F

H

H

F
|
F

G
x
A

V

N

S
x
P

V

E

E
x
V

R

R

P

T

F

T

E

E

E

P

I

I

L

V

-

H

-

F

G

N

A
x
E

N

N

I

V

S

V

G

A

V

T

F

F

H

N

I

V

Y

L

E

E

A

W

A

A

R

R

K

Q

H

T

G

G

V

V

K

R

R

T

V

V

I

V

F

F

A
|
A

S
|
S

N
x
S

H
x
T

V

V

I

Y

G

G

F

D

Y

A

K

D

Q

V

D

I

E

P

T

T

I

P

D

E

A

E

L

-

S

E

P

P

R

Y

R

K

P

P

D

I

G

S

Y

V

Y
|
Y

G

G

L

A

S

A

K
|
K

S

A

Y

A

G

G

E

E

D

V

V

M

A

C

S

A

F

T

Y

Y

F

A

D

R

R

L

Y

F

G

G

I

V

E

R

T

C

V

L

S

A

I

V

R

R

active site: A105 (= A117), S107 (= S119), S108 (≠ N120), T109 (≠ H121), Y131 (= Y144), K135 (= K148)
binding galactose-uridine-5'-diphosphate: P69 (≠ S83), V71 (≠ E85), S107 (= S119), Y131 (= Y144)
binding nicotinamide-adenine-dinucleotide: G7 (= G17), G10 (= G20), F11 (≠ G21), I12 (≠ L22), D31 (= D41), I32 (= I42), R46 (≠ C58), D47 (= D59), L48 (= L60), F65 (= F79), A66 (≠ G80), A67 (≠ G81), E82 (≠ A94), A105 (= A117), S106 (= S118), Y131 (= Y144), K135 (= K148)

Sites not aligning to the query:

active site: 166, 169
binding galactose-uridine-5'-diphosphate: 160, 168, 170, 173, 188, 193, 195, 197, 234, 263, 266
binding nicotinamide-adenine-dinucleotide: 158, 160, 161, 168

2c59A Gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), with gdp-alpha- d-mannose and gdp-beta-l-galactose bound in the active site. (see paper)
28% identity, 59% coverage: 12:172/273 of query aligns to 18:188/364 of 2c59A

query
sites
2c59A

R

K

L

I

L

S

L

I

T

T

G
|
G

A

A

A

G

G
|
G

G
x
F

L
x
I

G

A

K

S

V

H

L

I

R

A

E

R

R

R

L

L

K

K

P

H

F

E

A

G

R

H

H

Y

I

V

R

I

L

A

S

S

D
|
D

I
x
W

A
x
K

N

K

M

N

A

E

P

H

A

M

I

T

D

E

E

D

-

M

-

F

S

C

E

D

E

E

V

F

M

H

P

L

C

V

D
|
D

L
|
L

S

R

D

V

K

M

Q

E

A

N

V

C

H

L

Q

K

L

V

V

T

E

E

G

G

V

V

D

D

A

H

I

V

L

F

H

N

F
x
L

-

A

-
x
A

-

D

-
x
M

G
|
G

V
x
M

S

G

-

F

V
x
I

E

Q

R

S

P

N

F

H

E

S

E

V

I

I

L

M

G

Y

A

N

N

N

I

T

S

M

G
x
I

V

S

F

F

H

N

I

M

Y

I

E

E

A

A

R

R

K

I

H

N

G

G

V

I

K

K

R

R

V

F

I

F

F

Y

A
|
A

S

S

S
|
S

N
x
A

H
x
C

V

I

I

Y

G

P

F

E

Y

F

K

K

Q

Q

D

L

E

E

T

T

I

T

D

N

A

V

-

S

-

L

-

K

-

E

-

S

-

D

L

A

S

W

P

P

R

A

R

E

P

P

D

Q

G

D

Y

A

Y
|
Y

G

G

L

L

S

E

K
|
K

S

L

Y

A

G

T

E

E

D

E

V

L

A

C

S

K

F

H

Y

Y

F

N

D

K

R

D

Y

F

G

G

I

I

E

E

T

C

V

-

S

-

I

-

R

R

I

I

G

G

active site: S132 (= S119), A133 (≠ N120), C134 (≠ H121), Y163 (= Y144), K167 (= K148)
binding guanosine-5'-diphosphate-beta-l-galactose: M91 (vs. gap), G92 (= G80), G93 (= G81), M94 (≠ V82), S132 (= S119), C134 (≠ H121)
binding guanosine-5'-diphosphate-alpha-d-mannose: G92 (= G80), M94 (≠ V82), I97 (≠ V84), S132 (= S119), C134 (≠ H121), Y163 (= Y144)
binding nicotinamide-adenine-dinucleotide: G23 (= G17), G26 (= G20), F27 (≠ G21), I28 (≠ L22), D47 (= D41), W48 (≠ I42), K49 (≠ A43), D67 (= D59), L68 (= L60), L87 (≠ F79), A89 (vs. gap), M91 (vs. gap), I111 (≠ G98), A130 (= A117), S132 (= S119), Y163 (= Y144), K167 (= K148)

Sites not aligning to the query:

active site: 206
binding guanosine-5'-diphosphate-beta-l-galactose: 191, 192, 206, 207, 210, 211, 214, 225, 230, 232, 266, 289, 290, 345
binding guanosine-5'-diphosphate-alpha-d-mannose: 192, 206, 207, 210, 211, 214, 225, 230, 232, 266, 289, 290, 295, 345
binding nicotinamide-adenine-dinucleotide: 190, 192, 193, 206

Q93VR3 GDP-mannose 3,5-epimerase; GDP-Man 3,5-epimerase; EC 5.1.3.18 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 59% coverage: 12:172/273 of query aligns to 29:199/377 of Q93VR3

query
sites
Q93VR3

R

K

L

I

L

S

L

I

T

T

G
|
G

A
|
A

A
x
G

G
|
G

G
x
F

L
x
I

G
x
A

K
x
S

V
x
H

L
x
I

R
x
A

E
x
R

R
|
R

L
|
L

K
|
K

P
x
H

F
x
E

A
x
G

R
x
H

H
x
Y

I
x
V

R
x
I

L
x
A

S
|
S

D
|
D

I
x
W

A
x
K

N

K

M

N

A

E

P

H

A

M

I

T

D

E

E

D

-

M

-

F

S

C

E

D

E

E

V

F

M

H

P

L

C

V

D
|
D

L

L

S

R

D

V

K

M

Q

E

A

N

V

C

H

L

Q

K

L

V

V

T

E

E

G

G

V

V

D

D

A

H

I

V

L

F

H

N

F

L

-

A

-

D

-

M

G

G

V

M

S

G

-

F

V

I

E

Q

R

S

P

N

F

H

E

S

E

V

I

I

L

M

G

Y

A

N

N

N

I

T

S

M

G

I

V

S

F

F

H

N

I

M

Y

I

E

E

A

A

R

R

K

I

H

N

G

G

V

I

K

K

R

R

V

F

I

F

F

Y

A

A

S

S

N

A

H
x
C

V

I

I

Y

G

P

F

E

Y

F

K

K

Q

Q

D

L

E

E

T

T

I

T

D

N

A

V

-

S

-

L

-

K

-

E

-

S

-

D

L

A

S

W

P

P

R

A

R

E

P

P

D

Q

G

D

Y

A

Y
|
Y

G

G

L

L

S

E

K
|
K

S

L

Y

A

G

T

E

E

D

E

V

L

A

C

S

K

F

H

Y

Y

F

N

D

K

R

D

Y

F

G

G

I

I

E

E

T

C

V

-

S

-

I

-

R

R

I

I

G

G

34:60 (vs. 17:43, 30% identical) binding
D58 (= D41) binding
D78 (= D59) binding
C145 (≠ H121) mutation to A: Loss of activity.; mutation to S: Strong reduction of activity.
Y174 (= Y144) binding ; mutation to F: Loss of activity.
K178 (= K148) binding ; mutation to R: Strong reduction of activity.

Sites not aligning to the query:

217 K→A: Loss of activity.
306 R→A: Strong reduction of activity.

2c54A Gdp-mannose-3', 5' -epimerase (arabidopsis thaliana),k178r, with gdp- beta-l-gulose and gdp-4-keto-beta-l-gulose bound in active site. (see paper)
28% identity, 59% coverage: 12:172/273 of query aligns to 17:187/362 of 2c54A

query
sites
2c54A

R

K

L

I

L

S

L

I

T

T

G
|
G

A

A

A

G

G
|
G

G
x
F

L
x
I

G

A

K

S

V

H

L

I

R

A

E

R

R

R

L

L

K

K

P

H

F

E

A

G

R

H

H

Y

I

V

R

I

L

A

S

S

D
|
D

I
x
W

A
x
K

N

K

M

N

A

E

P

H

A

M

I

T

D

E

E

D

-

M

-

F

S

C

E

D

E

E

V

F

M

H

P

L

C
x
V

D
|
D

L
|
L

S

R

D

V

K

M

Q

E

A

N

V

C

H

L

Q

K

L

V

V

T

E

E

G

G

V

V

D

D

A

H

I

V

L

F

H

N

F
x
L

-

A

-
x
A

-

D

-
x
M

G
|
G

V
x
M

S

G

-

F

V

I

E

Q

R

S

P

N

F

H

E

S

E

V

I

I

L

M

G

Y

A

N

N

N

I

T

S

M

G
x
I

V

S

F

F

H

N

I

M

Y

I

E

E

A

A

R

R

K

I

H

N

G

G

V

I

K

K

R

R

V

F

I

F

F

Y

A
|
A

S

S

S
|
S

N
x
A

H
x
C

V

I

I

Y

G

P

F

E

Y

F

K

K

Q

Q

D

L

E

E

T

T

I

T

D

N

A

V

-

S

-

L

-

K

-

E

-

S

-

D

L

A

S

W

P

P

R

A

R

E

P

P

D

Q

G

D

Y

A

Y
|
Y

G

G

L

L

S

E

K
x
R

S

L

Y

A

G

T

E

E

D

E

V

L

A

C

S

K

F

H

Y

Y

F

N

D

K

R

D

Y

F

G

G

I

I

E

E

T

C

V

-

S

-

I

-

R

R

I

I

G

G

active site: S131 (= S119), A132 (≠ N120), C133 (≠ H121), Y162 (= Y144), R166 (≠ K148)
binding guanosine 5'-diphosphate-4-keto-beta-l-gulose: G91 (= G80), G92 (= G81), M93 (≠ V82), S131 (= S119)
binding guanosine 5'-diphosphate-beta-l-gulose: G91 (= G80), G92 (= G81), M93 (≠ V82), S131 (= S119), C133 (≠ H121), Y162 (= Y144)
binding nicotinamide-adenine-dinucleotide: G22 (= G17), G25 (= G20), F26 (≠ G21), I27 (≠ L22), D46 (= D41), W47 (≠ I42), K48 (≠ A43), V65 (≠ C58), D66 (= D59), L67 (= L60), L86 (≠ F79), A88 (vs. gap), M90 (vs. gap), I110 (≠ G98), A129 (= A117), S131 (= S119), Y162 (= Y144), R166 (≠ K148)

Sites not aligning to the query:

active site: 205
binding guanosine 5'-diphosphate-4-keto-beta-l-gulose: 190, 191, 205, 206, 209, 210, 213, 224, 229, 231, 265, 288, 289, 344
binding guanosine 5'-diphosphate-beta-l-gulose: 190, 191, 205, 206, 209, 210, 213, 224, 229, 231, 265, 288, 289, 344
binding nicotinamide-adenine-dinucleotide: 189, 191, 192, 205

5u4qB 1.5 angstrom resolution crystal structure of NAD-dependent epimerase from klebsiella pneumoniae in complex with NAD.
27% identity, 60% coverage: 12:175/273 of query aligns to 3:181/304 of 5u4qB

query
sites
5u4qB

R

K

L

F

L

L

L

V

T

T

G
|
G

A

A

G
|
G

G
x
F

L
x
I

G

G

K

F

V

H

L

I

R

A

E

Q

R

R

L

L

K

L

P

N

F

E

A

G

R

H

H

N

I

V

R

-

L

-

S

V

D

G

I

I

A
x
D

N
|
N

M

M

A
x
N

P

D

A

Y

I
x
Y

D

D

E

V

S

S

E

L

E
x
K

-

Q

-

A

-

R

-

L

-

D

-

R

-

L

-

A

-

Y

-

P

-

A

-

F

-

H

V

F

M

Q

P

Q

C

L

D
|
D

L
|
L

S

A

D

D

K

R

Q

E

A

G

V

M

H

A

Q

K

L

L

-

F

-

A

V

T

E

E

G

Q

V

F

D

D

A

R

I

V

L

I

H

H

F
x
L

G
x
A

-
x
A

-

Q

-

A

G

G

V

V

S

R

V

L

E

E

R

N

P

P

F

Y

E

A

E

-

I

Y

L

A

G

D

A

A

N

N

I

L

S

M

G

G

V

Y

F

L

H

N

I

I

Y

L

E

E

A

G

A

C

R

R

K

H

H

T

G

K

V

V

K

K

R

H

V

L

I

V

F

Y

A
|
A

S

S

N

S

H

S

V

V

I

Y

G

G

F

L

Y

N

K

R

Q

K

D

M

E

P

T

F

I

S

D

T

A

E

L

D

S

S

P

V

R

D

R

H

P

P

D

V

G

S

Y

L

Y
|
Y

G

A

L

A

S

T

K
|
K

S

K

Y

A

G

N

E

E

D

L

V

M

A

A

S

H

F

T

Y

Y

F

S

D

H

R

L

Y

Y

G

G

I

I

E

P

T

T

V

T

S

G

I

L

R

R

I
x
F

G

F

S

T

S
x
V

F

Y

binding nicotinamide-adenine-dinucleotide: G8 (= G17), G11 (= G20), F12 (≠ G21), I13 (≠ L22), D32 (≠ A43), N33 (= N44), N35 (≠ A46), Y38 (≠ I49), K43 (≠ E54), D61 (= D59), L62 (= L60), L83 (≠ F79), A84 (≠ G80), A85 (vs. gap), A123 (= A117), Y150 (= Y144), K154 (= K148), F177 (≠ I171), V180 (≠ S174)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 186, 189

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
28% identity, 62% coverage: 12:180/273 of query aligns to 2:179/307 of 6wjaA

query
sites
6wjaA

R

R

L

I

L

L

L

V

T

T

G
|
G

A

G

A

A

G
|
G

G
x
F

L
x
I

G

G

K

S

V

H

L

L

R

V

E

D

R

A

L

L

K

L

P

A

F

K

A

G

R

Y

H

A

I

V

R

R

L

V

S

L

D
|
D

-
x
D

-

L

-
x
S

-
x
T

-
x
G

-

K

I

V

A

G

N

N

M

L

A

-

P

P

A

M

I

G

D

D

E

A

S

G

E

L

E

E

V

L

M

L

P

V

C

G

D

D

L
x
A

S

A

D

D

K

A

Q

A

A

L

V

L

H

A

Q

D

L

A

V

V

E

Q

G

G

V

C

D

D

A

A

I

V

L

V

H

H

F
x
L

G
x
A

V

V

-

A

S

S

V
|
V

E

Q

R

A

P

S

F

V

E

E

E

D

I

P

L

V

G

A

-

T

-

H

-

Q

A
x
S

N

N

I

F

S

I

G

A

V

T

F

L

H

R

I

L

Y

C

E

E

A

A

A

M

R

T

K

A

H

A

G

G

V

I

K

R

R

R

V

V

I

V

F

F

A

A

S

S

S
x
A

N
x
A

H
x
A

V

V

I

Y

G

G

F

N

Y

N

K

G

Q

E

D

G

E

T

T

P

I

I

D

A

A

E

L

D

S

T

P

P

R

K

R

S

P

P

D

L

G

T

Y

P

Y
x
F

G

A

L

A

S

D

K
|
K

S

L

Y

A

G

S

E

E

D

Y

V

Y

A

L

S

D

F

F

Y

Y

F

R

D

R

R

Q

Y

H

G

G

I

L

E

E

T

P

V

V

S

I

I

L

R

R

I
x
F

G
x
F

S
x
N

S
x
I

F

F

P

G

E

P

P

R

Q

Q

N

D

active site: A118 (≠ S119), A119 (≠ N120), A120 (≠ H121), F143 (≠ Y144), K147 (= K148)
binding nicotinamide-adenine-dinucleotide: G7 (= G17), G10 (= G20), F11 (≠ G21), I12 (≠ L22), D31 (= D41), D32 (vs. gap), S34 (vs. gap), T35 (vs. gap), G36 (vs. gap), A55 (≠ L60), L74 (≠ F79), A75 (≠ G80), A76 (≠ G81), S93 (≠ A94), F143 (≠ Y144), K147 (= K148), F170 (≠ I171), F171 (≠ G172), I173 (≠ S174)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (= V84), A120 (≠ H121), N172 (≠ S173)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylgalactosamine: 186, 187, 191, 202, 204, 211, 247, 270, 273

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
28% identity, 62% coverage: 12:180/273 of query aligns to 3:180/308 of 6wj9B

query
sites
6wj9B

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active site: A119 (≠ S119), A120 (≠ N120), A121 (≠ H121), F144 (≠ Y144), K148 (= K148)
binding nicotinamide-adenine-dinucleotide: G8 (= G17), G11 (= G20), F12 (≠ G21), I13 (≠ L22), D32 (= D41), D33 (vs. gap), S35 (vs. gap), T36 (vs. gap), G37 (vs. gap), D55 (= D59), A56 (≠ L60), L75 (≠ F79), A76 (≠ G80), A77 (≠ G81), S94 (≠ A94), A117 (= A117), A119 (≠ S119), F144 (≠ Y144), K148 (= K148), F171 (≠ I171), F172 (≠ G172), I174 (≠ S174)
binding uridine-diphosphate-n-acetylglucosamine: V81 (= V84), N173 (≠ S173)

Sites not aligning to the query:

binding uridine-diphosphate-n-acetylglucosamine: 187, 188, 192, 203, 204, 205, 212, 248, 271, 274

5z75A Artificial l-threonine 3-dehydrogenase designed by ancestral sequence reconstruction. (see paper)
25% identity, 58% coverage: 12:170/273 of query aligns to 4:166/306 of 5z75A

query
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5z75A

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binding nicotinamide-adenine-dinucleotide: G9 (= G17), C11 (≠ A19), G12 (= G20), Q13 (≠ G21), I14 (≠ L22), D35 (= D41), I36 (= I42), L49 (≠ C58), V51 (≠ L60), L72 (≠ F79), A73 (≠ G80), L76 (≠ S83), P113 (≠ A117), S115 (= S119), Y140 (= Y144), K144 (= K148)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 167, 168, 170, 176, 177

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
30% identity, 60% coverage: 12:175/273 of query aligns to 2:182/322 of 1r6dA

query
sites
1r6dA

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active site: T127 (≠ S119), N128 (= N120), Q129 (≠ H121), Y151 (= Y144), K155 (= K148)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (= S83), H88 (vs. gap), T127 (≠ S119), N128 (= N120), Q129 (≠ H121), Y151 (= Y144), N180 (≠ S173)
binding nicotinamide-adenine-dinucleotide: G10 (= G20), F11 (≠ G21), I12 (≠ L22), D37 (≠ S40), S38 (≠ D41), L39 (≠ I42), T40 (vs. gap), A42 (vs. gap), G43 (vs. gap), D63 (= D59), I64 (≠ L60), F83 (= F79), A84 (≠ G80), A85 (≠ G81), S87 (= S83), T102 (vs. gap), V125 (≠ A117), S126 (= S118), Y151 (= Y144), K155 (= K148), N181 (≠ S174)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 190, 191, 206, 208, 215, 250, 274, 277, 281

Query Sequence

>Pf6N2E2_489 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_489
MTTTQIRHPFNRLLLTGAAGGLGKVLRERLKPFARHIRLSDIANMAPAIDESEEVMPCDL
SDKQAVHQLVEGVDAILHFGGVSVERPFEEILGANISGVFHIYEAARKHGVKRVIFASSN
HVIGFYKQDETIDALSPRRPDGYYGLSKSYGEDVASFYFDRYGIETVSIRIGSSFPEPQN
RRMMSTWLSFDDLTQLIECSLYTPKVGHTVVYGMSANRDVWWDNSHAAHLGYQPKDTSEI
FRAKVEAQPMPAADDPAMVYQGGAFVAAGPFDD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory