SitesBLAST

Q9X5C9 Quinate/shikimate dehydrogenase (NAD(+)); QSDH; EC 1.1.1.-; EC 1.1.1.24 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
51% identity, 98% coverage: 5:283/285 of query aligns to 2:283/283 of Q9X5C9

query
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Q9X5C9

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S17 (= S20) binding shikimate
SRT 17:19 (= SRT 20:22) binding L-quinate
T69 (= T72) binding L-quinate; binding shikimate
K73 (= K76) active site, Proton acceptor; binding L-quinate; binding shikimate
N94 (= N97) binding L-quinate; binding shikimate
D110 (= D112) binding L-quinate; binding shikimate
GV 137:138 (≠ GA 139:140) binding NAD(+)
D158 (= D160) binding NAD(+)
R163 (= R165) binding NAD(+)
PMGM 203:206 (= PMGM 203:206) binding NAD(+)
A213 (≠ P213) binding NAD(+)
V228 (≠ I228) binding NAD(+)
G251 (= G251) binding NAD(+)
Q258 (= Q258) binding L-quinate; binding shikimate

Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / DSM 28319 / BCRC 17069 / CCUG 31568 / BM 3577 / RP62A) (see paper)
30% identity, 93% coverage: 12:276/285 of query aligns to 5:257/269 of Q5HNV1

query
sites
Q5HNV1

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SLS 13:15 (≠ SRT 20:22) binding shikimate
T60 (= T72) binding shikimate
N85 (= N97) binding shikimate
D100 (= D112) binding shikimate
Y211 (= Y230) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
Q239 (= Q258) binding shikimate

Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
34% identity, 95% coverage: 7:276/285 of query aligns to 6:272/282 of Q58484

query
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Q58484

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GAGGA 130:134 (= GAGGA 136:140) binding NADP(+)
NRTVEK 152:157 (≠ DVEVSR 160:165) binding NADP(+)
T196 (= T202) binding NADP(+)
M200 (= M206) binding NADP(+)
L224 (≠ I228) binding NADP(+)

1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
34% identity, 95% coverage: 7:276/285 of query aligns to 11:277/287 of 1nvtB

query
sites
1nvtB

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active site: K75 (= K76), D111 (= D112)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ F73), G135 (= G136), G137 (= G138), G138 (= G139), A139 (= A140), N157 (≠ D160), R158 (≠ V161), T159 (≠ E162), K162 (≠ R165), A200 (≠ T201), T201 (= T202), P202 (= P203), I203 (≠ M204), M205 (= M206), L229 (≠ I228), Y231 (= Y230), M255 (= M254), L256 (≠ A255)
binding zinc ion: E22 (≠ Q18), H23 (≠ A19)

1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
34% identity, 95% coverage: 7:276/285 of query aligns to 11:277/287 of 1nvtA

query
sites
1nvtA

T

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L

L

K

K

-

Y

-

V

-

I

-

D

A

G

A

A

E

K

R

A

M

L

S

G

F

I

T

V

G

G

L

F

N

N

I

V

T

T

F

I

P

P

C

H

K
|
K

Q

I

A

E

I

I

I

M

P

K

L

Y

L

L

D

D

E

E

L

I

S

D

P

K

E

D

A

A

R

Q

G

L

I

I

G

G

A

A

V

V

N

N

T

T

V

I

V

K

L

I

K

E

D

D

G

G

K

K

R

A

I

I

G

G

H

Y

N

N

T

T

D
|
D

C

G

L

I

G

G

F

A

A

R

E

M

G

A

F

L

R

E

G

E

L

I

G

G

D

R

V

V

A

K

R

D

R

K

R

N

V

I

V

V

Q

I

M

Y

G
|
G

A

A

G

G

A
|
A

G

A

A

R

A

A

V

V

A

A

H

F

A

E

L

L

L

A

A

K

E

D

G

-

V

-

Q

N

T

N

L

I

S

I

I

I

F

A

D
x
N

V
x
R

E
x
T

V

V

S

E

R
x
K

A

A

E

E

A

A

L

L

A

A

N

K

-

E

-

I

-

A

-

E

N

K

L

L

N

N

Q

K

H

K

F

F

G

G

V

E

G

E

R

V

A

K

R

F

A

S

G

G

H

L

D

D

L

V

S

D

A

-

M

L

A

D

E

G

A

V

D

D

G

I

L

I

V

I

N

N

T
x
A

T
|
T

P
|
P

M
x
I

G

G

M
|
M

A

-

K

-

L

Y

P

P

G

N

M

I

P

D

V

V

-

E

P

P

V

I

-

V

-

K

-

A

E

E

L

K

L

L

H

R

G

E

Q

D

L

M

W

V

V

V

A

M

E

D

I
x
L

V

I

Y
|
Y

F

N

P

P

L

L

E

E

T

T

E

V

L

L

R

K

N

E

A

A

R

K

A

K

L

V

G

N

C

A

R

K

T

T

L

I

D

N

G
|
G

G

L

N

G

M
|
M

A
x
L

V

I

F

Y

Q

Q

A

G

V

A

K

V

A

A

F

F

E

K

L

I

F

W

S

T

G

G

V

V

A

E

P

P

D

N

A

I

Q

E

R

V

M

M

active site: K75 (= K76), D111 (= D112)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G136), A139 (= A140), N157 (≠ D160), R158 (≠ V161), T159 (≠ E162), K162 (≠ R165), A200 (≠ T201), T201 (= T202), P202 (= P203), I203 (≠ M204), M205 (= M206), L229 (≠ I228), Y231 (= Y230), G252 (= G251), M255 (= M254), L256 (≠ A255)

3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
29% identity, 93% coverage: 12:276/285 of query aligns to 5:248/258 of 3dooA

query
sites
3dooA

L

V

I

I

G

G

A

N

G

P

I

I

Q

S

A

H

S
|
S

R

L

T
x
S

P

P

A

L

L

M

H

H

E

H

H

A

E

N

G

F

D

Q

A

S

Q

L

G

N

M

L

R

E

Y

N

L

T

Y

Y

R

-

L

-

I

-

D

-

L

-

D

E

A

A

L

I

K

N

L

V

D

P

S

V

G

N

A

Q

L

F

P

Q

D

D

L

I

L

K

K

K

A

I

E

S

R

E

M

K

S

S

F

I

T

D

G

G

L

F

N
|
N

I

V

T
|
T

F

I

P

P

C

H

K
|
K

Q

E

A

R

I

I

P

P

L

Y

L

L

D

D

E

D

L

I

S

N

P

E

E

Q

A

A

R

K

G

S

I

V

G

G

A

A

V

V

N
|
N

T

T

V

V

V

L

L

V

K

K

D

D

G

G

K

K

R

W

I

I

G

G

H

Y

N

N

T

T

D
|
D

C

G

L

I

G

G

F

Y

A

V

E

N

G

G

F

L

R

K

R

Q

G

I

L

Y

G

E

D

G

V

I

A

E

R

D

R

A

R

Y

V

I

V

L

Q

I

M

L

G

G

A

A

G

G

A

A

G

S

A

K

A

G

V

I

A

A

H

N

A

E

L

L

L

Y

A

K

E

I

G

V

V

R

Q

P

T

T

L

L

S

T

I

V

F

A

D

N

V

R

E

T

V

M

S

S

R

R

A

F

E

N

A

N

L

W

A

S

N

L

N

N

L

I

N

N

Q

K

H

-

F

I

G

N

V

L

G

S

R

H

A

A

R

E

A

S

G

H

H

L

D

D

L

-

S

-

A

-

M

-

A

-

E

E

A

F

D

D

G

I

L

I

V

I

N

N

T

T

P

P

M

V

G

-

M

-

A

-

K

-

L

-

P

-

G

-

M

-

P

-

V

I

P

S

V

L

E

N

L

R

L

L

H

A

G

S

Q

H

L

T

W

L

V

V

A

S

E

D

I

I

V

V

Y

Y

F

N

P

P

L

Y

E

K

T

T

E

P

L

I

L

L

R

I

N

E

A

A

R

E

A

Q

L

R

G

G

C

N

R

P

T

I

L

Y

D

N

G

G

G

L

N

D

M

M

A
x
F

V

V

F

H

Q
|
Q

A

G

V

A

K

E

A

S

F

F

E

K

L

I

F

W

S

T

G

N

V

L

A

E

P

P

D

D

A

I

Q

K

R

A

M

M

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S20), S15 (≠ T22), N58 (= N70), T60 (= T72), K64 (= K76), N85 (= N97), D100 (= D112), F227 (≠ A255), Q230 (= Q258)

3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
32% identity, 92% coverage: 7:267/285 of query aligns to 13:277/291 of 3tozA

query
sites
3tozA

T

T

V

E

L

L

A

I

G

G

L

L

I

I

G

A

A

T

G

P

I

I

Q

R

A

H

S

S

R

L

T

S

P

P

A

T

L

M

H

H

E

N

H

E

E

A

G

F

D

A

A

K

Q

L

G

G

M

L

R

D

Y

Y

L

V

Y

Y

R

L

L

-

I

-

D

-

L

-

D

-

A

A

L

F

K

E

L

V

D

G

S

D

G

K

A

E

L

L

P

K

D

D

L

V

K

Q

A

G

A

F

E

R

R

A

M

M

S

N

F

L

T

R

G

G

L

W

N

N

I

V

T

S

F

M

P

P

C

N

K

K

Q

T

A

N

I

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

P

P

E

A

A

A

R

E

G

L

I

V

G

G

A

A

V

V

N

N

T

T

V

V

L

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D

D

C

G

L

T

G

G

F

Y

A

M

E

R

G

A

F

L

R

K

R

E

G

A

L

G

G

H

D

D

V

I

A

I

R

G

R

K

V

M

V

T

Q

I

M

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

L

A

A

L

E

D

G

G

V

V

Q

K

T

E

L

I

S

S

I

I

F

F

-
x
N

-
x
R

-

K

D
|
D

V

D

E
x
F

V

Y

S

A

R

N

A

A

E

E

A

K

L

T

A

V

N

E

N

K

L

I

N

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

Q

Q

H

L

F

F

G

D

V

I

G

-

R

-

A

-

R

E

A

D

G

H

H

E

D

Q

L

L

S

R

A

K

A

E

M

I

A

A

E

E

A

S

D

V

G

I

L

F

V

T

N

N

T

A

T
|
T

P
x
G

M
x
V

G

G

M
|
M

A

K

K

P

L
x
F

P

E

G

G

M

E

P

T

V

L

-

L

-

P

P

S

V

A

E

D

L

M

L

L

H

R

G

P

Q

E

L

L

W

I

V

V

A

S

E

D

I
x
V

V

V

Y
|
Y

F

K

P

P

L

T

E

K

T

T

E

R

L

L

R

E

N

I

A

A

R

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

D

N

G
|
G

G

L

N

G

M
|
M

A
x
M

V

L

F

W

Q

Q

A

G

V

A

K

K

A

A

F

F

E

E

L

I

F

W

S

T

binding nicotinamide-adenine-dinucleotide: G137 (= G136), A138 (= A137), G139 (= G138), G140 (= G139), A141 (= A140), N161 (vs. gap), R162 (vs. gap), D164 (= D160), F166 (≠ E162), T210 (= T202), G211 (≠ P203), V212 (≠ M204), M214 (= M206), F217 (≠ L209), V238 (≠ I228), Y240 (= Y230), G261 (= G251), M264 (= M254), M265 (≠ A255)

Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
32% identity, 92% coverage: 7:267/285 of query aligns to 13:277/291 of Q8Y9N5

query
sites
Q8Y9N5

T

T

V

E

L

L

A

I

G

G

L

L

I

I

G

A

A

T

G

P

I

I

Q

R

A

H

S
|
S

R
x
L

T
x
S

P

P

A

T

L

M

H

H

E

N

H

E

E

A

G

F

D

A

A

K

Q

L

G

G

M

L

R

D

Y

Y

L

V

Y

Y

R

L

L

-

I

-

D

-

L

-

D

-

A

A

L

F

K

E

L

V

D

G

S

D

G

K

A

E

L

L

P

K

D

D

L

V

K

Q

A

G

A

F

E

R

R

A

M

M

S

N

F

L

T

R

G

G

L

W

N

N

I

V

T

S

F

M

P

P

C

N

K

K

Q

T

A

N

I

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

P

P

E

A

A

A

R

E

G

L

I

V

G

G

A

A

V

V

N

N

T

T

V

V

L

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D

D

C

G

L

T

G

G

F

Y

A

M

E

R

G

A

F

L

R

K

R

E

G

A

L

G

G

H

D

D

V

I

A

I

R

G

R

K

V

M

V

T

Q

I

M

C

G

G

A

A

G

G

A

A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

L

A

A

L

E

D

G

G

V

V

Q

K

T

E

L

I

S

S

I

I

F

F

-
x
N

-
x
R

-
x
K

D
|
D

V

D

E

F

V

Y

S

A

R

N

A

A

E

E

A

K

L

T

A

V

N

E

N

K

L

I

N

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

Q

Q

H

L

F

F

G

D

V

I

G

-

R

-

A

-

R

E

A

D

G

H

H

E

D

Q

L

L

S

R

A

K

A

E

M

I

A

A

E

E

A

S

D

V

G

I

L

F

V

T

N

N

T

A

T

T

P

G

M

V

G

G

M
|
M

A

K

K

P

L

F

P

E

G

G

M

E

P

T

V

L

-

L

-

P

P

S

V

A

E

D

L

M

L

L

H

R

G

P

Q

E

L

L

W

I

V

V

A

S

E

D

I

V

V

V

Y

Y

F

K

P

P

L

T

E

K

T

T

E

R

L

L

R

E

N

I

A

A

R

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

D

N

G

G

G

L

N

G

M

M

A

M

V

L

F

W

Q

Q

A

G

V

A

K

K

A

A

F

F

E

E

L

I

F

W

S

T

SLS 26:28 (≠ SRT 20:22) binding shikimate
NRKD 161:164 (≠ ---D 160) binding NAD(+)
M214 (= M206) binding NADP(+)

3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
32% identity, 92% coverage: 7:267/285 of query aligns to 10:274/288 of 3tnlA

query
sites
3tnlA

T

T

V

E

L

L

A

I

G

G

L

L

I

I

G

A

A

T

G

P

I

I

Q

R

A

H

S
|
S

R

L

T
x
S

P

P

A

T

L

M

H

H

E

N

H

E

E

A

G

F

D

A

A

K

Q

L

G

G

M

L

R

D

Y

Y

L

V

Y

Y

R

L

L

-

I

-

D

-

L

-

D

-

A

A

L

F

K

E

L

V

D

G

S

D

G

K

A

E

L

L

P

K

D

D

L

V

K

Q

A

G

A

F

E

R

R

A

M

M

S

N

F

L

T

R

G

G

L

W

N
|
N

I

V

T
x
S

F
x
M

P

P

C

N

K
|
K

Q

T

A

N

I

I

I

H

P

K

L

Y

L

L

D

D

E

K

L

L

S

S

P

P

E

A

A

A

R

E

G

L

I

V

G

G

A

A

V

V

N
|
N

T

T

V

V

L

N

K

D

D

D

G

G

K

V

R

L

I

T

G

G

H

H

N

I

T

T

D
|
D

C

G

L

T

G

G

F

Y

A

M

E

R

G

A

F

L

R

K

R

E

G

A

L

G

G

H

D

D

V

I

A

I

R

G

R

K

V

M

V

T

Q

I

M

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

A

T

A

A

V

I

A

C

H

I

A

Q

L

A

A

L

E

D

G

G

V

V

Q

K

T

E

L

I

S

S

I

I

F

F

-
x
N

-
x
R

-

K

D
|
D

V

D

E
x
F

V

Y

S

A

R

N

A

A

E

E

A

K

L

T

A

V

N

E

N

K

L

I

N

N

-

S

-

K

-

T

-

D

-

C

-

K

-

A

Q

Q

H

L

F

F

G

D

V

I

G

-

R

-

A

-

R

E

A

D

G

H

H

E

D

Q

L

L

S

R

A

K

A

E

M

I

A

A

E

E

A

S

D

V

G

I

L

F

V

T

N

N

T

A

T
|
T

P

G

M
x
V

G

G

M
|
M

A

K

K

P

L
x
F

P

E

G

G

M

E

P

T

V

L

-

L

-

P

P

S

V

A

E

D

L

M

L

L

H

R

G

P

Q

E

L

L

W

I

V

V

A

S

E

D

I
x
V

V

V

Y
|
Y

F

K

P

P

L

T

E

K

T

T

E

R

L

L

R

E

N

I

A

A

R

E

A

E

L

Q

G

G

C

C

R

Q

T

T

L

L

D

N

G

G

G

L

N

G

M
|
M

A
x
M

V

L

F

W

Q
|
Q

A

G

V

A

K

K

A

A

F

F

E

E

L

I

F

W

S

T

binding nicotinamide-adenine-dinucleotide: M71 (≠ F73), G134 (= G136), A135 (= A137), G136 (= G138), G137 (= G139), A138 (= A140), N158 (vs. gap), R159 (vs. gap), D161 (= D160), F163 (≠ E162), T207 (= T202), V209 (≠ M204), M211 (= M206), F214 (≠ L209), V235 (≠ I228), Y237 (= Y230), M261 (= M254), M262 (≠ A255)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S20), S25 (≠ T22), N68 (= N70), S70 (≠ T72), K74 (= K76), N95 (= N97), D110 (= D112), Q265 (= Q258)

1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
30% identity, 91% coverage: 9:268/285 of query aligns to 9:272/288 of 1npdB

query
sites
1npdB

L

L

A

I

G

G

L

L

I

M

G

A

A

Y

G

P

I

I

Q

R

A

H

S

S

R

L

T

S

P

P

A

E

L

M

H

Q

E

N

H

K

E

A

G

L

D

E

A

K

Q

A

G

G

M

L

R

P

Y

F

L

T

Y

Y

R

M

L

-

I

-

D

-

L

-

D

-

A

A

L

F

K

E

L

V

D

D

S

N

G

D

A

S

L

F

P

P

D

G

L

A

L

I

K

E

A

G

A

L

E

K

R

A

M

L

S

K

F

M

T

R

G

G

L

T

N

G

I

V

T

S

F

M

P

P

C

N

K

K

Q

Q

A

L

I

A

I

C

P

E

L

Y

L

V

D

D

E

E

L

L

S

T

P

P

E

A

A

A

R

K

G

L

I

V

G

G

A

A

V

I

N

N

T

T

V

I

V

V

L

N

K

D

D

D

G

G

K

Y

R

L

I

R

G

G

H

Y

N

N

T

T

D

D

C

G

L

T

G

G

F

H

A

I

E

R

G

A

F

I

R

K

R

E

G

S

L

G

G

F

D

D

V

I

A

K

R

G

R

K

R

T

V

M

V

V

Q

L

M

L

G

G

A
|
A

G
|
G

A
|
A

G

S

A

T

A

A

V

I

A

G

H

A

A

Q

L

G

L

A

A

I

E

E

G

G

V

L

Q

K

T

E

L

I

S

K

I

L

F

F

-
x
N

-
x
R

-

R

D
|
D

V

E

E
x
F

V

F

S

D

R

K

A

A

E

L

A

A

L

F

A

A

N

Q

N

R

L

V

N

N

Q

E

H

N

-

T

-

D

-

C

-

V

F

V

G

T

V

V

G

T

R

D

A

L

R

A

A

D

G

Q

H

Q

D

A

L

F

S

A

A

E

A

A

M

L

A

A

E

S

A

A

D

D

G

I

L

L

V

T

N

N

T

G

T
|
T

P
x
K

M
x
V

G

G

M
|
M

A

K

K

P

L

L

P

E

G

N

M

E

P

S

V

L

-

V

-

N

P

D

V

I

E

S

L

L

H

H

G

P

Q

G

L

L

W

L

V

V

A

T

E

E

I
x
C

V

V

Y

Y

F

N

P

P

L

H

E

M

T

T

E

K

L

L

R

Q

N

Q

A

A

R

Q

A

Q

L

A

G

G

C

C

R

K

T

T

L

I

D

D

G

G

G

Y

N

G

M
|
M

A
x
L

V

L

F

W

Q

Q

A

G

V

A

K

E

A

Q

F

F

E

T

L

L

F

W

S

T

G

G

binding nicotinamide-adenine-dinucleotide: A132 (= A137), G133 (= G138), G134 (= G139), A135 (= A140), N155 (vs. gap), R156 (vs. gap), D158 (= D160), F160 (≠ E162), T204 (= T202), K205 (≠ P203), V206 (≠ M204), M208 (= M206), C232 (≠ I228), M258 (= M254), L259 (≠ A255)

P0A6D5 Quinate/shikimate dehydrogenase; NAD-dependent shikimate 5-dehydrogenase; EC 1.1.1.282 from Escherichia coli (strain K12) (see 4 papers)
30% identity, 91% coverage: 9:268/285 of query aligns to 9:272/288 of P0A6D5

query
sites
P0A6D5

L

L

A

I

G

G

L

L

I

M

G

A

A

Y

G

P

I

I

Q

R

A

H

S

S

R

L

T
x
S

P

P

A

E

L

M

H

Q

E

N

H

K

E

A

G

L

D

E

A

K

Q

A

G

G

M

L

R

P

Y

F

L

T

Y
|
Y

R

M

L

-

I

-

D

-

L

-

D

-

A

A

L

F

K

E

L

V

D

D

S

N

G

D

A

S

L

F

P

P

D

G

L

A

L

I

K

E

A

G

A

L

E

K

R

A

M

L

S

K

F

M

T

R

G

G

L

T

N

G

I

V

T
x
S

F

M

P

P

C

N

K
|
K

Q

Q

A

L

I

A

I

C

P

E

L

Y

L

V

D

D

E

E

L

L

S

T

P

P

E

A

A

A

R

K

G

L

I

V

G

G

A

A

V

I

N
|
N

T

T

V

I

V

V

L

N

K

D

D

D

G

G

K

Y

R

L

I

R

G

G

H

Y

N

N

T
|
T

D
|
D

C

G

L

T

G

G

F

H

A

I

E

R

G

A

F

I

R

K

R

E

G

S

L

G

G

F

D

D

V

I

A

K

R

G

R

K

R

T

V

M

V

V

Q

L

M

L

G

G

A
|
A

G
|
G

A
|
A

G

S

A

T

A

A

V

I

A

G

H

A

A

Q

L

G

L

A

A

I

E

E

G

G

V

L

Q

K

T

E

L

I

S

K

I

L

F

F

-
x
N

-
x
R

D
|
D

V

E

E

F

V

F

S

D

R

K

A

A

E

L

A

A

L

F

A

A

N

Q

N

R

L

V

N

N

Q

E

H

N

-

T

-

D

-

C

-

V

F

V

G

T

V

V

G

T

R

D

A

L

R

A

A

D

G

Q

H

Q

D

A

L

F

S

A

A

E

A

A

M

L

A

A

E

S

A

A

D

D

G

I

L

L

V

T

N

N

T

G

T

T

P
x
K

M

V

G

G

M

M

A

K

K

P

L

L

P

E

G

N

M

E

P

S

V

L

-

V

-

N

P

D

V

I

E

S

L

L

H

H

G

P

Q

G

L

L

W

L

V

V

A

T

E

E

I
x
C

V
|
V

Y
|
Y

F
x
N

P

P

L

H

E

M

T

T

E

K

L

L

R

Q

N

Q

A

A

R

Q

A

Q

L

A

G

G

C

C

R

K

T

T

L

I

D

D

G
|
G

G

Y

N

G

M

M

A

L

V

L

F

W

Q
|
Q

A

G

V

A

K

E

A

Q

F

F

E

T

L

L

F

W

S

T

G

G

S22 (≠ T22) mutation to A: Kinetically unchanged as compared with the wild-type.
Y39 (= Y39) mutation to F: Kinetically unchanged as compared with the wild-type.
S67 (≠ T72) mutation to A: Reduces activity towards quinate about 6-fold, but has a little effect on shikimate conversion.
K71 (= K76) mutation to A: 3200-fold decrease in the affinity for quinate. 170-fold decrease in the affinity for shikimate.; mutation to G: 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
N92 (= N97) mutation to A: Alters protein structure. Loss of activity for both substrates.
T106 (= T111) mutation to A: 2000-fold decrease in the affinity for quinate. 70-fold decrease in the affinity for shikimate. 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
D107 (= D112) mutation to A: Loss of activity towards quinate. 20000-fold decrease in the affinity for shikimate.
AGGA 132:135 (= AGGA 137:140) binding NAD(+)
NRRD 155:158 (≠ ---D 160) binding NAD(+)
K205 (≠ P203) binding NAD(+)
CVYN 232:235 (≠ IVYF 228:231) binding NAD(+)
G255 (= G251) binding NAD(+)
Q262 (= Q258) mutation to A: 3-fold reduction in catalytic efficiency for both substrates.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
31% identity, 92% coverage: 7:268/285 of query aligns to 6:252/269 of 2hk9A

query
sites
2hk9A

T

T

V

Q

L

L

A

Y

G

G

L

V

I

I

G

G

A

F

G

P

I

V

Q

K

A

H

S
|
S

R

L

T
x
S

P

P

A

V

L

F

H

Q

E

N

H

A

E

-

G

-

D

-

A

-

Q

-

G

-

M

-

R

-

Y

-

L

L

Y

I

R

R

L

Y

I

A

D

G

L

L

D

N

A

A

L

V

K

Y

L

L

D

A

S

F

G

E

A

I

L

N

P

P

D

E

L

E

L

L

K

K

A

K

A

A

-

F

-

E

-

G

-

F

E

K

R

A

M

L

S

K

F

V

T

K

G

G

L

I

N
|
N

I

V

T

T

F
x
V

P

P

C

F

K
|
K

Q

E

A

E

I

I

P

P

L

L

D

D

E

Y

L

V

S

E

P

D

E

T

A

A

R

K

G

E

I

I

G

G

A

A

V

V

N
|
N

T

T

V

V

V

K

L

F

K

E

D

N

G

G

K

K

R

A

I

Y

G

G

H

Y

N

N

T

T

D
|
D

C

W

L

I

G

G

F

F

A

L

E

K

G

S

F

L

R

K

R

S

G

L

L

I

G

P

D

E

V

V

A

K

R

E

R

K

R

S

V

I

V

L

Q

V

M

L

G

G

A

A

G
|
G

A
|
A

G

S

A

R

A

A

V

V

A

I

H

Y

A

A

L

L

L

V

A

K

E

E

G

G

V

A

Q

K

T

V

L

F

S

L

I

W

F

-

D

-

V

-

E

-

V

-

S
x
N

R
|
R

A
x
T

E

K

A

E

L

K

A

A

N

I

N

K

L

L

N

A

Q

Q

H

K

F

F

G

P

V

L

G

E

R

V

A

V

R

N

A

S

G

P

H

E

D

E

L

V

S

-

A

-

M

I

A

D

E

K

A

V

D

Q

G

V

L

I

V

V

N

N

T

T

T
|
T

P
x
S

M
x
V

G

G

M

L

A

K

K

D

L

E

P

D

G

P

M

E

P

I

V

F

P

N

V

Y

E

D

L

L

L

I

H

K

G

K

Q

D

L

H

W

V

V

V

A

V

E

D

I

I

V

I

Y
|
Y

F

-

P

-

L

K

E

E

T

T

E

K

L

L

R

K

N

K

A

A

R

K

A

E

L

K

G

G

C

A

R

K

T

L

L

L

D

D

G

G

G

L

N

P

M

M

A
x
L

V

L

F

W

Q
|
Q

A

G

V

I

K

E

A

A

F

F

E

K

L

I

F

W

S

N

G

G

binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ F73), G132 (= G138), G133 (= G139), A134 (= A140), N153 (≠ S164), R154 (= R165), T155 (≠ A166), T188 (= T202), S189 (≠ P203), V190 (≠ M204)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S20), S21 (≠ T22), N64 (= N70), K70 (= K76), N91 (= N97), D106 (= D112), Y216 (= Y230), L239 (≠ A255), Q242 (= Q258)

2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
31% identity, 92% coverage: 7:268/285 of query aligns to 6:252/267 of 2hk9B

query
sites
2hk9B

T

T

V

Q

L

L

A

Y

G

G

L

V

I

I

G

G

A

F

G

P

I

V

Q

K

A

H

S
|
S

R

L

T
x
S

P

P

A

V

L

F

H

Q

E

N

H

A

E

-

G

-

D

-

A

-

Q

-

G

-

M

-

R

-

Y

-

L

L

Y

I

R

R

L

Y

I

A

D

G

L

L

D

N

A

A

L

V

K

Y

L

L

D

A

S

F

G

E

A

I

L

N

P

P

D

E

L

E

L

L

K

K

A

K

A

A

-

F

-

E

-

G

-

F

E

K

R

A

M

L

S

K

F

V

T

K

G

G

L

I

N
|
N

I

V

T
|
T

F
x
V

P

P

C

F

K
|
K

Q

E

A

E

I

I

P

P

L

L

D

D

E

Y

L

V

S

E

P

D

E

T

A

A

R

K

G

E

I

I

G

G

A

A

V

V

N
|
N

T

T

V

V

V

K

L

F

K

E

D

N

G

G

K

K

R

A

I

Y

G

G

H

Y

N

N

T

T

D
|
D

C

W

L

I

G

G

F

F

A

L

E

K

G

S

F

L

R

K

R

S

G

L

L

I

G

P

D

E

V

V

A

K

R

E

R

K

R

S

V

I

V

L

Q

V

M

L

G
|
G

A

A

G

G

G
|
G

A
|
A

G

S

A

R

A

A

V

V

A

I