SitesBLAST
Comparing Pf6N2E2_5159 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5159 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
50% identity, 97% coverage: 2:284/293 of query aligns to 14:296/308 of P27305
- E55 (= E43) binding
- Y182 (= Y170) binding
- R200 (= R188) binding
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
50% identity, 97% coverage: 2:284/293 of query aligns to 2:282/290 of 4a91A
- active site: S11 (≠ T11), K229 (= K229)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (≠ T11), E43 (= E43), Y170 (= Y170), R188 (= R188), L192 (= L192)
- binding zinc ion: C99 (= C99), C101 (= C101), Y113 (= Y113), C117 (= C117)
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
37% identity, 77% coverage: 7:231/293 of query aligns to 6:242/468 of 8i9iA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
37% identity, 77% coverage: 7:231/293 of query aligns to 6:242/471 of P04805
- C98 (= C99) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C101) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C117) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ N119) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ G121) mutation to Q: No change in activity or in zinc content.
- H131 (= H122) mutation to Q: No change in activity or in zinc content.
- H132 (≠ G123) mutation to Q: No change in activity or in zinc content.
- C138 (≠ A127) mutation to S: No change in activity or in zinc content.
- S239 (≠ G228) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
37% identity, 76% coverage: 7:229/293 of query aligns to 5:250/484 of 2cfoA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
37% identity, 76% coverage: 7:229/293 of query aligns to 6:251/485 of Q8DLI5
- R6 (= R7) binding
- Y192 (= Y170) binding
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 2cv2A
- active site: K246 (= K229)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (≠ T11), G17 (= G19), I21 (≠ A23), E41 (= E43), Y187 (= Y170), R205 (= R188), A206 (≠ G189), E208 (≠ D191), W209 (≠ L192), L235 (= L218), L236 (≠ I219)
- binding : S9 (≠ T11), T43 (≠ L45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (= R149), Y168 (vs. gap), E172 (= E155), V177 (= V160), K180 (≠ R163), S181 (≠ R164), Y187 (= Y170), E207 (≠ A190), E208 (≠ D191), W209 (≠ L192), V211 (≠ D194), R237 (≠ T220), K241 (≠ G224)
Sites not aligning to the query:
- binding : 272, 273, 274, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 2cv1A
- active site: K246 (= K229)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (≠ T11), G17 (= G19), T18 (≠ S20), I21 (≠ A23), R47 (= R49), A206 (≠ G189), W209 (≠ L192), L235 (= L218), L236 (≠ I219)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E43), Y187 (= Y170), R205 (= R188), W209 (≠ L192)
- binding : S9 (≠ T11), E41 (= E43), T43 (≠ L45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (= R149), V166 (≠ L152), E172 (= E155), V177 (= V160), K180 (≠ R163), S181 (≠ R164), Y187 (= Y170), E207 (≠ A190), E208 (≠ D191), W209 (≠ L192), V211 (≠ D194), R237 (≠ T220), K241 (≠ G224), K243 (= K226)
Sites not aligning to the query:
- binding : 273, 274, 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l-glutamate (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 1n78A
- active site: K246 (= K229)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (≠ T11), G17 (= G19), T18 (≠ S20), I21 (≠ A23), E41 (= E43), Y187 (= Y170), N191 (≠ V174), R205 (= R188), A206 (≠ G189), E208 (≠ D191), W209 (≠ L192), L235 (= L218), L236 (≠ I219)
- binding : S9 (≠ T11), T43 (≠ L45), D44 (= D46), R47 (= R49), V145 (≠ A128), R163 (= R149), V166 (≠ L152), Y168 (vs. gap), E172 (= E155), V177 (= V160), K180 (≠ R163), S181 (≠ R164), Y187 (= Y170), E207 (≠ A190), E208 (≠ D191), W209 (≠ L192), L210 (= L193), V211 (≠ D194), R237 (≠ T220), K241 (≠ G224)
Sites not aligning to the query:
- binding : 273, 274, 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 1j09A
- active site: K246 (= K229)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D191), L235 (= L218), L236 (≠ I219), K243 (= K226), I244 (≠ L227), S245 (≠ G228), K246 (= K229), R247 (≠ S230)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (≠ T11), E41 (= E43), Y187 (= Y170), N191 (≠ V174), R205 (= R188), W209 (≠ L192)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of P27000
Sites not aligning to the query:
- 358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
35% identity, 84% coverage: 5:250/293 of query aligns to 3:267/468 of 1g59A
- binding : D44 (= D46), R45 (≠ P47), A46 (≠ P48), R47 (= R49), P109 (≠ R103), V145 (≠ A128), R163 (= R149), V166 (≠ L152), E172 (= E155), V177 (= V160), K180 (≠ R163), S181 (≠ R164), D182 (= D165), E207 (≠ A190), E208 (≠ D191), R237 (≠ T220), K241 (≠ G224), T242 (≠ H225), K243 (= K226)
Sites not aligning to the query:
- binding : 273, 274, 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
32% identity, 83% coverage: 7:249/293 of query aligns to 106:355/564 of 3al0C
- active site: S110 (≠ T11), K335 (= K229)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (≠ A23), E142 (= E43), Y276 (= Y170), R294 (= R188), G295 (= G189), D297 (= D191), H298 (≠ L192), L324 (= L218), I325 (= I219), L333 (= L227)
- binding : T144 (≠ L45), D145 (= D46), R148 (= R49), Y208 (≠ C101), P213 (vs. gap), K252 (≠ Q145), M255 (≠ R149), I266 (≠ V160), K269 (≠ R163), S270 (≠ R164), Y276 (= Y170), D297 (= D191), H298 (≠ L192), L299 (= L193), S300 (≠ D194), N301 (≠ S195), K304 (≠ R198), R330 (≠ G224), P332 (≠ K226)
Sites not aligning to the query:
- binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
32% identity, 77% coverage: 7:231/293 of query aligns to 6:227/380 of 4g6zA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
32% identity, 78% coverage: 7:236/293 of query aligns to 5:260/485 of 4griB
- active site: S9 (≠ T11), K253 (= K229)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (≠ T11), E41 (= E43), Y194 (= Y170), R212 (= R188), W216 (≠ L192)
- binding zinc ion: C105 (= C99), C107 (= C101), Y128 (= Y113), C132 (= C117)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
33% identity, 78% coverage: 1:229/293 of query aligns to 1:254/488 of 8vc5A
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid
30% identity, 76% coverage: 7:229/293 of query aligns to 6:261/502 of 6brlA
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
32% identity, 71% coverage: 7:215/293 of query aligns to 14:228/455 of 3aiiA
Sites not aligning to the query:
2re8A Glutaminyl-tRNA synthetase mutant c229r with bound analog 5'-o-[n-(l- glutamyl)-sulfamoyl]adenosine (see paper)
29% identity, 38% coverage: 2:111/293 of query aligns to 18:127/529 of 2re8A
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: P25 (≠ A9), P26 (= P10), E27 (≠ T11), G35 (= G19), H36 (≠ S20), D59 (≠ E43)
- binding : E27 (≠ T11), T61 (≠ L45), N62 (≠ D46), L117 (≠ C101), P119 (≠ R103), R123 (≠ E107), R126 (≠ Q110)
Sites not aligning to the query:
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: 204, 208, 222, 223, 253, 254, 263
- binding : 6, 128, 129, 161, 174, 175, 176, 182, 185, 203, 204, 226, 228, 306, 309, 310, 311, 313, 314, 316, 319, 322, 329, 334, 362, 363, 392, 394, 395, 403, 405, 406, 435, 499, 501, 502, 527
Query Sequence
>Pf6N2E2_5159 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5159
MTAYIGRFAPTPSGHLHFGSLVAALASYLDARAMGGRWLLRMEDLDPPREEPGAQAAILK
ALESYGFEWDGEMVRQSERHDAYDQVINRLFSQGLAYACTCSRKQLEAYQGIYPGLCRNA
GHGTEDAAIRLRVPELEYHFTDRVQGEFRQHLGREVGDFVIRRRDGLYAYQLAVVLDDAW
QGVTDIVRGADLLDSTPRQLYLQELLGLPQPRYLHVPLITQPDGHKLGKSYRSPPLAADQ
ATPLLLRALRALGQQPGLELVDASPRELLAWGIRHWDAGKIPRTLSLPEAQIR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory