SitesBLAST
Comparing Pf6N2E2_518 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_518 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
63% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt5A
- active site: K97 (= K97), H176 (= H176)
- binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K97), H155 (= H155), H176 (= H176), Y235 (= Y235)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), E96 (= E96), K97 (= K97), Y280 (= Y280)
3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
63% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt4A
- active site: K97 (= K97), H176 (= H176)
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (= H155), H176 (= H176), Y235 (= Y235), W272 (= W272)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), E96 (= E96), K97 (= K97), H176 (= H176)
3nt2B Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
63% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt2B
- active site: K97 (= K97), H176 (= H176)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), A78 (≠ S78), K97 (= K97), W272 (= W272), Y280 (= Y280)
3nt2A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
63% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 3nt2A
- active site: K97 (= K97), H176 (= H176)
- binding nicotinamide-adenine-dinucleotide: G9 (= G9), G11 (= G11), A12 (= A12), I13 (= I13), D35 (= D35), V36 (≠ I36), S74 (= S74), W75 (= W75), G76 (= G76), A78 (≠ S78), H79 (= H79), E96 (= E96), K97 (= K97), H176 (= H176), Y280 (= Y280)
4l8vA Crystal structure of a12k/d35s mutant myo-inositol dehydrogenase from bacillus subtilis with bound cofactor NADP (see paper)
62% identity, 100% coverage: 1:335/336 of query aligns to 1:336/337 of 4l8vA
- active site: K97 (= K97), H176 (= H176)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ A12), I13 (= I13), S74 (= S74), W75 (= W75), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), W272 (= W272), Y280 (= Y280)
3ec7A Crystal structure of putative dehydrogenase from salmonella typhimurium lt2
52% identity, 100% coverage: 1:335/336 of query aligns to 1:335/336 of 3ec7A
- active site: K97 (= K97), H176 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), M12 (≠ A12), I13 (= I13), D35 (= D35), I36 (= I36), R40 (≠ Q40), T73 (= T73), A74 (≠ S74), S75 (≠ W75), N76 (≠ G76), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), W271 (= W272), Y279 (= Y280)
4mjlD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD and d-chiro-inositol
47% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mjlD
- active site: K97 (= K97)
- binding (1r,2r,3s,4s,5s,6s)-cyclohexane-1,2,3,4,5,6-hexol: K97 (= K97), H155 (= H155), N157 (= N157), D172 (= D172), T173 (= T173), H176 (= H176), Y236 (= Y235)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), V73 (≠ T73), S74 (= S74), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), D172 (= D172), W274 (= W272), Y282 (= Y280)
4mioD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
47% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mioD
- active site: K97 (= K97)
- binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: K97 (= K97), H155 (= H155), N157 (= N157), D172 (= D172), T173 (= T173), H176 (= H176), Y236 (= Y235)
- binding 1,4-dihydronicotinamide adenine dinucleotide: A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), S74 (= S74), A78 (≠ S78), H79 (= H79), E96 (= E96), K97 (= K97), M126 (= M126), D172 (= D172), W274 (= W272), Y282 (= Y280)
4mioA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
47% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4mioA
- active site: K97 (= K97)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), S74 (= S74), F75 (≠ W75), H79 (= H79), E96 (= E96), K97 (= K97), W274 (= W272), Y282 (= Y280)
4minA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD
47% identity, 100% coverage: 1:335/336 of query aligns to 1:338/339 of 4minA
- active site: K97 (= K97)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), A12 (= A12), M13 (≠ I13), D35 (= D35), I36 (= I36), V73 (≠ T73), S74 (= S74), F75 (≠ W75), H79 (= H79), E96 (= E96), K97 (= K97), Y282 (= Y280)
4n54A Crystal structure of scyllo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD(h) and scyllo-inositol
25% identity, 97% coverage: 2:326/336 of query aligns to 2:338/340 of 4n54A
- active site: K96 (= K97), H183 (= H176)
- binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: R12 (≠ A12), K96 (= K97), D156 (≠ N157), D179 (= D172), M180 (≠ T173), H183 (= H176), R238 (= R219), Y244 (= Y235)
- binding 1,4-dihydronicotinamide adenine dinucleotide: V8 (≠ I8), G9 (= G9), L10 (≠ T10), G11 (= G11), R12 (≠ A12), L13 (≠ I13), S35 (≠ D35), V36 (≠ I36), E40 (≠ N37), S73 (= S74), P74 (≠ W75), F77 (≠ S78), H78 (= H79), E95 (= E96), K96 (= K97), M125 (= M126), F167 (≠ M168), F284 (≠ W272)
3ceaA Crystal structure of myo-inositol 2-dehydrogenase (np_786804.1) from lactobacillus plantarum at 2.40 a resolution
26% identity, 97% coverage: 3:328/336 of query aligns to 5:342/342 of 3ceaA
- active site: K98 (= K97), H185 (= H176)
- binding nicotinamide-adenine-dinucleotide: G11 (= G9), G13 (= G11), R14 (≠ A12), L15 (≠ I13), L38 (vs. gap), Q42 (≠ L38), V74 (≠ T73), A75 (≠ S74), P76 (≠ W75), T77 (≠ G76), F79 (≠ S78), H80 (= H79), M83 (≠ F82), E97 (= E96), K98 (= K97), M127 (= M126), F169 (≠ M168), H185 (= H176), F286 (≠ W272)
7xr9A Crystal structure of dgpa with glucose (see paper)
31% identity, 48% coverage: 35:196/336 of query aligns to 29:200/344 of 7xr9A
- binding beta-D-glucopyranose: K91 (= K97), R150 (≠ H155), W157 (≠ V160), F160 (vs. gap), D173 (= D172), H177 (= H176)
- binding nicotinamide-adenine-dinucleotide: D51 (= D57), Y52 (≠ G58), C67 (≠ T73), T68 (≠ S74), P69 (≠ W75), H73 (= H79), E90 (= E96), K91 (= K97), P92 (= P98), Q119 (≠ M126), V159 (vs. gap), F160 (vs. gap), Q166 (≠ K165)
Sites not aligning to the query:
Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
35% identity, 38% coverage: 61:188/336 of query aligns to 58:192/333 of Q2I8V6