SitesBLAST
Comparing Pf6N2E2_5889 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5889 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
81% identity, 98% coverage: 5:253/253 of query aligns to 3:251/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G16 (= G18), I17 (= I19), D36 (= D38), L37 (= L39), C61 (= C63), D62 (≠ N64), V63 (= V65), N89 (= N91), A90 (= A92), T140 (= T142), S142 (= S144), Y155 (= Y157), K159 (= K161), A186 (= A188), V187 (= V189)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
43% identity, 98% coverage: 5:252/253 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G18), S142 (= S144), Y155 (= Y157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), S15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), A61 (≠ C63), D62 (≠ N64), T63 (≠ V65), N89 (= N91), A90 (= A92), M140 (≠ T142), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (= A188), Y187 (≠ V189), I188 (= I190), L192 (≠ M194)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 3:252/253 of query aligns to 1:247/248 of 4urfB
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ V215), R211 (≠ G216), R212 (= R217)
- binding bicarbonate ion: I92 (= I94), G94 (≠ I96), R109 (≠ A111), R179 (= R181), S228 (= S233)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), D62 (≠ N64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T142), Y155 (= Y157), K159 (= K161), P185 (= P187), A186 (= A188), I188 (= I190), T190 (= T192)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 3:252/253 of query aligns to 1:247/248 of 4urfA
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (= I94), S93 (≠ E95), G94 (≠ I96), E95 (= E97), T97 (≠ G99), E101 (≠ D103), T103 (= T105), Q106 (≠ E108), R109 (≠ A111), S175 (≠ K177), G177 (≠ K179)
- binding magnesium ion: S237 (≠ H242), Y238 (≠ S243)
- binding nicotinamide-adenine-dinucleotide: G12 (= G14), G14 (≠ A16), N15 (≠ A17), G16 (= G18), I17 (= I19), D36 (= D38), I37 (≠ L39), W41 (≠ G43), D62 (≠ N64), T63 (≠ V65), N89 (= N91), A90 (= A92), G91 (= G93), I140 (≠ T142), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
41% identity, 99% coverage: 3:252/253 of query aligns to 1:247/248 of 4ureB
- active site: G16 (= G18), S142 (= S144), I152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ A17), G16 (= G18), I17 (= I19), N89 (= N91), G91 (= G93), Y155 (= Y157), P185 (= P187), A186 (= A188)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
40% identity, 100% coverage: 1:252/253 of query aligns to 1:254/255 of 5itvA
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (≠ L39), T61 (≠ C63), I63 (≠ V65), N89 (= N91), G91 (= G93), T139 (= T142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (≠ A188), I186 (≠ V189), I187 (= I190)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
37% identity, 100% coverage: 1:252/253 of query aligns to 4:252/258 of 4wecA
- active site: G21 (= G18), S143 (= S144), Q154 (≠ M154), Y157 (= Y157), K161 (= K161)
- binding nicotinamide-adenine-dinucleotide: G17 (= G14), A19 (= A16), S20 (≠ A17), G21 (= G18), I22 (= I19), D41 (= D38), I42 (≠ L39), V61 (≠ C63), D62 (≠ N64), V63 (= V65), N89 (= N91), T141 (= T142), Y157 (= Y157), K161 (= K161), P187 (= P187), P189 (≠ V189), V190 (≠ I190)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
41% identity, 96% coverage: 8:250/253 of query aligns to 8:242/244 of 4nbuB
- active site: G18 (= G18), N111 (= N116), S139 (= S144), Q149 (≠ M154), Y152 (= Y157), K156 (= K161)
- binding acetoacetyl-coenzyme a: D93 (≠ K98), K98 (≠ D103), S139 (= S144), N146 (≠ A151), V147 (≠ P152), Q149 (≠ M154), Y152 (= Y157), F184 (≠ V189), M189 (= M194), K200 (≠ F208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), F39 (≠ L39), V59 (≠ C63), D60 (≠ N64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (= I94), T137 (= T142), S139 (= S144), Y152 (= Y157), K156 (= K161), P182 (= P187), F184 (≠ V189), T185 (≠ I190), T187 (= T192), M189 (= M194)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
39% identity, 100% coverage: 1:252/253 of query aligns to 1:226/227 of 5itvD
- active site: G18 (= G18), S141 (= S144), Y154 (= Y157), K158 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ A17), G18 (= G18), I19 (= I19), D38 (= D38), I39 (≠ L39), T61 (≠ C63), D62 (≠ N64), I63 (≠ V65), N89 (= N91), T139 (= T142), S141 (= S144), Y154 (= Y157), K158 (= K161), P184 (= P187), G185 (≠ A188), I187 (= I190)
2dtxA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with d-mannose (see paper)
42% identity, 96% coverage: 8:251/253 of query aligns to 8:246/255 of 2dtxA
2dteA Structure of thermoplasma acidophilum aldohexose dehydrogenase (aldt) in complex with nadh (see paper)
42% identity, 96% coverage: 8:251/253 of query aligns to 8:246/255 of 2dteA
- active site: G18 (= G18), S132 (= S144), Y145 (= Y157), S148 (= S160), K149 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S16 (≠ A16), M17 (≠ A17), G18 (= G18), I19 (= I19), S38 (= S50), I39 (= I51), C52 (= C63), D53 (≠ N64), V54 (= V65), N80 (= N91), A81 (= A92), I130 (≠ T142), S132 (= S144), Y145 (= Y157), K149 (= K161), P174 (= P187), A175 (= A188), T176 (≠ V189), I177 (= I190), T179 (= T192), P180 (≠ D193), L181 (≠ M194), V182 (≠ F195)
4qecA Elxo with NADP bound (see paper)
34% identity, 94% coverage: 11:249/253 of query aligns to 6:244/248 of 4qecA
- active site: G13 (= G18), N111 (= N116), S139 (= S144), Y152 (= Y157), K156 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ A17), G13 (= G18), I14 (= I19), S33 (≠ D38), R34 (≠ L39), K38 (≠ G43), D59 (≠ N64), V60 (= V65), N86 (= N91), A87 (= A92), G88 (= G93), I137 (≠ T142), Y152 (= Y157), K156 (= K161), P182 (= P187), I185 (= I190)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
36% identity, 98% coverage: 5:252/253 of query aligns to 11:265/267 of Q9LBG2
- 17:42 (vs. 11:36, 50% identical) binding
- E103 (= E95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
37% identity, 98% coverage: 2:250/253 of query aligns to 3:245/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G14), R18 (≠ A17), G19 (= G18), I20 (= I19), D39 (= D38), R40 (≠ L39), C63 (= C63), I65 (≠ V65), N91 (= N91), G93 (= G93), I94 (= I94), V114 (= V115), Y155 (= Y157), K159 (= K161), I188 (= I190), T190 (= T192), T193 (≠ F195)
1iy8A Crystal structure of levodione reductase (see paper)
36% identity, 98% coverage: 5:252/253 of query aligns to 2:256/258 of 1iy8A
- active site: G15 (= G18), S143 (= S144), Q153 (≠ M154), Y156 (= Y157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (≠ A17), G15 (= G18), L16 (≠ I19), D35 (= D38), V36 (≠ L39), A62 (≠ C63), D63 (≠ N64), V64 (= V65), N90 (= N91), G92 (= G93), I93 (= I94), T141 (= T142), S143 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (≠ A188), T191 (= T192), P192 (≠ D193), M193 (= M194)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 98% coverage: 3:250/253 of query aligns to 1:245/247 of 4jroC
- active site: G16 (= G18), S142 (= S144), Q152 (≠ M154), Y155 (= Y157), K159 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G14), S14 (≠ A16), R15 (≠ A17), G16 (= G18), I17 (= I19), N35 (≠ A37), Y36 (vs. gap), N37 (≠ D38), G38 (≠ L39), S39 (≠ D40), N63 (= N64), V64 (= V65), N90 (= N91), A91 (= A92), I93 (= I94), I113 (≠ V115), S142 (= S144), Y155 (= Y157), K159 (= K161), P185 (= P187), I188 (= I190), T190 (= T192)
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
41% identity, 98% coverage: 5:252/253 of query aligns to 11:256/257 of 5h5xC
- active site: G24 (= G18), S151 (= S144), Y164 (= Y157), K168 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G14), S23 (≠ A17), G24 (= G18), I25 (= I19), D44 (= D38), F45 (≠ L39), L69 (≠ C63), D70 (≠ N64), N97 (= N91), A98 (= A92), Y164 (= Y157), K168 (= K161), P194 (= P187), G195 (≠ A188), I197 (= I190), T199 (= T192)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)-hydroxypropylethane thiosulfonate dehydrogenases (see paper)
40% identity, 97% coverage: 8:253/253 of query aligns to 3:250/250 of 2cfcA
- active site: G13 (= G18), S142 (= S144), Y155 (= Y157), K159 (= K161)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ A151), R152 (≠ M154), Y155 (= Y157), W195 (≠ R197), R196 (≠ A198)
- binding nicotinamide-adenine-dinucleotide: G9 (= G14), S12 (≠ A17), G13 (= G18), N14 (≠ I19), D33 (= D38), L34 (= L39), A59 (≠ C63), D60 (≠ N64), V61 (= V65), N87 (= N91), A88 (= A92), G89 (= G93), I140 (≠ T142), P185 (= P187), G186 (≠ A188), M187 (≠ V189), I188 (= I190), T190 (= T192), P191 (≠ D193), M192 (= M194), T193 (≠ F195)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
40% identity, 97% coverage: 8:253/253 of query aligns to 3:250/250 of Q56840
- SGN 12:14 (≠ AGI 17:19) binding
- D33 (= D38) binding
- DV 60:61 (≠ NV 64:65) binding
- N87 (= N91) binding
- S142 (= S144) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ M154) binding ; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y157) mutation Y->E,F: Loss of activity.
- K159 (= K161) mutation to A: Loss of activity.
- R179 (= R181) mutation to A: Loss of activity.
- IETPM 188:192 (≠ IDTDM 190:194) binding
- WR 195:196 (≠ RA 197:198) binding
- R196 (≠ A198) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (≠ G206) mutation to A: Slight decrease in catalytic efficiency.
- R209 (≠ M212) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
36% identity, 100% coverage: 1:252/253 of query aligns to 1:260/261 of 6zzsD
- active site: G18 (= G18), S143 (= S144), Y156 (= Y157)
- binding nicotinamide-adenine-dinucleotide: G14 (= G14), S17 (≠ A17), I19 (= I19), D38 (= D38), M39 (≠ L39), D64 (≠ N64), V65 (= V65), N91 (= N91), A92 (= A92), G93 (= G93), M141 (≠ T142), A142 (= A143), S143 (= S144), Y156 (= Y157), K160 (= K161), P186 (= P187), G187 (≠ A188), V189 (≠ I190), T191 (= T192), L193 (≠ M194)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ E95), S143 (= S144), N145 (≠ A146), K153 (≠ M154), Y156 (= Y157), Q197 (vs. gap)
Query Sequence
>Pf6N2E2_5889 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5889
MSMTFSGQVAVVTGAAAGIGRATALAFAAEGLKVVVADLDVAGGEGTVQSIRDAGGEAVF
VRCNVTLENDVQQLMDEVIKTYGRLDYAFNNAGIEIEKGKLADGTLDEFDAIMGVNVKGV
WLCMKYQLPLLLAQGGGAIVNTASVAGLGAAPKMSIYAASKHAVIGLTKSAAIEYAKKKI
RVNAVCPAVIDTDMFRRAYEADPKKGEFANAMHPVGRIGKVEEIASAVLYLCSDGAAFTT
GHSLAVDGGVTAF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory