SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_5969 L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
45% identity, 95% coverage: 16:504/514 of query aligns to 5:492/501 of P04983

query
sites
P04983

L

L

R

Q

F

L

N

K

G

G

I

I

G

D

K

K

S

A

F

F

P

P

G

G

V

V

Q

K

A

A

L

L

A

S

N

G

I

A

S

A

F

L

V

N

A

V

H

Y

P

P

G

G

Q

R

V

V

H

M

A

A

L

L

M

V

G

G

E

E

N

N

G

G

A

A

G

G

K
|
K

S

S

T

T

L

M

K

K

I

V

L

L

G

T

G

G

A

I

Y

Y

I

T

P

R

S

D

S

A

G

G

D

T

L

L

-

L

Q

W

I

L

G

G

E

K

Q

E

T

T

M

-

A

T

F

F

K

T

G

G

T

P

A

K

D

S

S

S

I

Q

A

E

S

A

G

G

V

I

A

G

V

I

I

I

H

H

Q

Q

E

E

L

L

H

N

L

L

V

I

P

P

E

Q

M

L

T

T

V

I

A

A

E

E

N

N

L

I

F

F

L

L

G

G

-

R

H

E

L

F

P

V

A

N

R

R

F

F

G

G

L

K

V

I

N

D

R

W

G

K

V

T

L

M

R

Y

Q

A

Q

E

A

A

L

D

T

K

L

L

K

A

G

K

L

L

A

N

D

L

E

R

I

F

D

K

P

S

Q

D

E

K

K

L

V

V

G

G

R

D

L

L

S

S

L

I

G

G

Q

D

R

Q

Q

Q

L

M

V

V

E

E

I

I

A

A

K

K

A

V

L

L

S

S

R

F

G

E

A

S

H

K

V

V

I

I

A

I

F

M

D

D

E

E

P

P

T

T

S

D

S

A

L

L

S

T

A

D

R

T

E

E

I

T

D

E

R

S

L

L

M

F

A

R

I

V

I

I

G

R

R

E

L

L

R

K

D

S

E

Q

G

G

K

R

V

G

V

I

L

V

Y

Y

V

I

S

S

H

H

R

R

M

M

E

K

E

E

V

I

F

F

R

E

I

I

C

C

N

D

A

D

V

V

T

T

V

V

F

F

K

R

D

D

G

G

R

Q

Y

F

V

I

R

A

T

E

F

R

E

E

N

-

M

V

S

A

E

S

L

L

T

T

H

E

D

D

Q

S

L

L

V

I

T

E

C

M

M

M

V

V

G

G

R

R

D

K

I

L

Q

E

D

D

I

Q

Y

Y

D

P

Y

H

R

L

P

D

R

K

E

A

R

P

G

G

D

D

V

I

A

R

L

L

Q

K

V

V

K

D

G

N

L

L

L

C

G

G

P

P

G

G

L

V

H

N

E

D

P

-

V

V

S

S

F

F

Q

T

V

L

H

R

K

K

G

G

E

E

I

I

L

L

G

G

L

V

F

S

G

G

L

L

V

M

G

G

A

A

G

G

R

R

T

T

E

E

L

L

L

M

R

K

L

V

L

L

S

Y

G

G

L

A

E

L

R

P

Q

R

R

T

E

S

G

G

S

Y

L

V

V

T

L

L

H

D

D

G

K

H

E

E

L

V

K

V

L

T

R

R

S

S

P

P

R

Q

D

D

A

G

I

L

A

A

A

N

G

G

V

I

L

V

L

Y

C

I

P

S

E

E

D

D

R

R

K

K

K

R

E

D

G

G

I

L

I

V

P

L

L

G

G

M

S

S

V

V

G

K

E

E

N

N

I

M

N

S

I

L

S

T

A

A

R

L

P

R

S

Y

H

F

S

S

T

R

L

A

G

G

C

G

L

S

L

L

R

K

G

H

D

A

W

D

E

E

R

Q

G

Q

N

A

A

V

D

S

K

D

Q

F

I

I

K

R

S

L

L

F

K

N

V

V

K

K

T

T

P

P

T

S

A

M

G

E

Q

Q

K

A

I

I

M

G

Y

L

L

L

S

S

G

G

N

N

Q

Q

K

K

A

V

I

A

L

I

G

A

R

R

W

G

L

L

S

M

M

T

P

R

M

P

K

K

V

V

L

L

L

I

L

L

D

D

E

E

P

P

T

T

R

R

G

G

I

V

D

D

I

V

G

G

A

A

K

K

A

K

E

E

I

I

Y

Y

Q

Q

I

L

I

I

H

N

N

Q

L

F

A

K

A

A

D

D

G

G

I

L

A

S

V

I

I

I

V

L

V

V

S

S

D

E

L

M

M

P

E

E

V

V

M

L

G

G

I

M

S

S

D

D

R

R

I

I

L

I

V

V

L

M

C

H

E

E

G

G

A

H

M

L

R

S

G

G

E

E

L

F

S

T

R

R

D

E

Q

Q

A

A

N

T

E

Q

S

E

N

V

L

L

L

M

Q

A

L

A

A

A

L

V

K43 (= K54) mutation to R: Loss of transport.

7arlD Lolcde in complex with lipoprotein and adp (see paper)
33% identity, 39% coverage: 31:228/514 of query aligns to 22:219/222 of 7arlD

query
sites
7arlD

L

L

A

H

N

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E

S

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

I

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

T

P

M

M

A

S

F

K

K

L

G

S

T

S

A

A

D

A

S

K

I

A

A

E

-

L

-

R

-

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q

Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

R

L

F

I

G

G

L

K

V

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

T

E

L

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D

R

P

A

Q

N

E

H

K

R

V

P

G

S

R

E

L

L

S

S

L

G

G

G

Q

E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H

H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

binding adenosine-5'-diphosphate: G42 (= G51), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 39% coverage: 31:228/514 of query aligns to 25:222/233 of P75957

query
sites
P75957

L

L

A

H

N

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G
|
G

E

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

I

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

T

P

M

M

A

S

F

K

K

L

G

S

T

S

A

A

D

A

S

K

I

A

A

E

-

L

-

R

-

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q

Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

R

L

F

I

G

G

L

K

V

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

T

E

L

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D

R

P

A

Q

N

E

H

K

R

V

P

G

S

R

E

L

L

S

S

L

G

G

G

Q

E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H

H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

G42 (= G48) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
33% identity, 39% coverage: 31:228/514 of query aligns to 22:219/226 of 7mdyC

query
sites
7mdyC

L

L

A

H

N

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E
x
S

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

I

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

T

P

M

M

A

S

F

K

K

L

G

S

T

S

A

A

D

A

S

K

I

A

A

E

-

L

-

R

-

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q
|
Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

R

L

F

I

G

G

L

K

V

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

T

E

L

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D
x
R

P

A

Q

N

E
x
H

K

R

V

P

G

S

R
x
E

L

L

S
|
S

L
x
G

G
|
G

Q
x
E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E
|
E

P

P

T

T

S

G

S
x
N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H
|
H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

binding adp orthovanadate: S40 (≠ E49), S41 (≠ N50), G42 (= G51), S43 (≠ A52), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56), Q91 (= Q97), R135 (≠ D144), H138 (≠ E147), E142 (≠ R151), S144 (= S153), G145 (≠ L154), G146 (= G155), E147 (≠ Q156), E168 (= E177), N172 (≠ S181), H201 (= H209)
binding magnesium ion: S46 (= S55), Q91 (= Q97)

Sites not aligning to the query:

binding adp orthovanadate: 12, 19, 21

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs
33% identity, 39% coverage: 31:228/514 of query aligns to 24:221/229 of 7v8iD

query
sites
7v8iD

L

L

A

H

N

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E

S

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

I

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

T

P

M

M

A

S

F

K

K

L

G

S

T

S

A

A

D

A

S

K

I

A

A

E

-

L

-

R

-

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q
|
Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

R

L

F

I

G

G

L

K

V

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

T

E

L

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D
x
R

P

A

Q

N

E
x
H

K

R

V

P

G

S

R
x
E

L
|
L

S
|
S

L
x
G

G
|
G

Q
x
E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

Q

P

P

T

T

S

G

S
x
N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H
|
H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

binding phosphoaminophosphonic acid-adenylate ester: S43 (≠ N50), G44 (= G51), S45 (≠ A52), G46 (= G53), K47 (= K54), S48 (= S55), T49 (= T56), Q93 (= Q97), R137 (≠ D144), H140 (≠ E147), E144 (≠ R151), L145 (= L152), S146 (= S153), G147 (≠ L154), G148 (= G155), E149 (≠ Q156), N174 (≠ S181), H203 (= H209)
binding magnesium ion: S48 (= S55), Q93 (= Q97)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 23

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
34% identity, 42% coverage: 16:231/514 of query aligns to 5:223/648 of P75831

query
sites
P75831

L

L

R

E

F

L

N

K

G

D

I

I

G

R

K

R

S

S

F

Y

P

P

G

A

-

G

-

D

-

E

-

Q

V

V

Q

E

A

V

L

L

A

K

N

G

I

I

S

S

F

L

V

D

A

I

H

Y

P

A

G

G

Q

E

V

M

H

V

A

A

L

I

M

V

G

G

E

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

L

M

K

N

I

I

L

L

G

G

G

C

A

L

Y

D

I

K

P

A

S

T

S

S

G

G

D

T

L

Y

Q

R

I

V

G

A

E

G

Q

Q

T

D

M

V

A

A

F

-

K

T

G

L

T

D

A

A

D

D

S

A

I

L

A

A

-

Q

-

L

-

R

S

E

G

H

V

F

A

G

V

F

I

I

H

F

Q

Q

E

R

L

Y

H

H

L

L

V

L

P

S

E

H

M

L

T

T

V

A

A

E

E

Q

N

N

L

V

F

-

L

-

G

-

H

E

L

V

P

P

A

A

R

V

F

Y

G

A

L

G

V

L

N

E

R

R

G

K

-

Q

-

R

V

L

L

L

R

R

Q

A

Q

Q

A

E

L

L

T

L

L

Q

L

R

K

L

G

G

L

L

A

E

D

D

E

R

I

T

D

E

P

Y

Q

Y

E

P

K

-

V

-

G

A

R

Q

L

L

S

S

L

G

G

G

Q

Q

R

Q

Q

Q

L

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

R

N

G

G

A

G

H

Q

V

V

I

I

A

L

F

A

D
|
D

E

E

P

P

T

T

S

G

S

A

L

L

S

D

A

S

R

H

E

S

I

G

D

E

R

E

L

V

M

M

A

A

I

I

I

L

G

H

R

Q

L

L

R

R

D

D

E

R

G

G

K

H

V

T

V

V

L

I

Y

I

V

V

S

T

H

H

R

D

M

-

E

P

E

Q

V

V

F

A

R

A

I

Q

C

A

N

E

A

R

V

V

T

I

V

E

F

I

K

R

D

D

G

G

R

E

Y

I

V

V

R

R

K47 (= K54) mutation to L: Lack of activity.
D169 (= D176) mutation to N: Lack of activity.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 41% coverage: 19:230/514 of query aligns to 5:215/240 of 4ymuJ

query
sites
4ymuJ

N

N

G

D

I

V

G

Y

K

K

S

N

F
|
F

P

G

G

S

V
x
L

Q

E

A
x
V

L

L

A

K

N

G

I

V

S

T

F

L

V

K

A

V

H

N

P

K

G

G

Q

E

V

V

H

V

A

V

L

I

M

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

C

L

I

G

N

G

L

A

L

Y
x
E

I

E

P

P

S

T

S

K

G

G

D

E

L

V

Q

F

I

I

-

D

G

G

E

V

Q

K

T

I

M

N

A

N

F

G

K

K

G

V

T

N

A

I

D

N

S

K

I

V

A

R

S

Q

G

K

V

V

A

G

V

M

I

V

H

F

Q

Q

E

H

L

F

H

N

L

L

V

F

P

P

E

H

M

L

T

T

V

A

A

I

E

E

N

N

L

I

F

T

L

L

G

A

H

-

L

-

P

P

A

V

R

K

F

V

G

K

L

K

V

M

N

N

R

K

G

K

V

E

L

A

R

E

Q

E

Q

L

A

A

L

V

T

D

L

L

K

A

-

K

-

V

G

G

L

L

A

L

D

D

E

K

I

K

D

D

P

-

Q

Q

E

Y

K

P

V

I

G

-

R

K

L

L

S

S

L

G

G

G

Q

Q

R

K

Q

Q

L

R

V

L

E

A

I

I

A

A

K

R

A

A

L

L

S

A

R

M

G

Q

A

P

H

E

V

V

I

M

A

L

F

F

D

D

E
|
E

P

P

T

T

S

S

S

A

L

L

S

D

A

P

R

E

E

M

I

V

D

K

R

E

L

V

M

L

A

N

I

V

I

M

G

K

R

Q

L

L

R

A

D

N

E

E

G

G

K

M

V

T

V

M

L

V

Y

V

V

V

S

T

H
|
H

R

E

M

M

E

G

E

F

V

A

F

R

R

E

I

V

C

G

N

D

A

R

V

V

T

I

V

F

F

M

K

D

D

D

G

G

R

V

Y

I

V

V

binding adenosine-5'-triphosphate: F11 (= F25), L14 (≠ V28), V16 (≠ A30), S36 (≠ N50), G37 (= G51), S38 (≠ A52), G39 (= G53), K40 (= K54), S41 (= S55), T42 (= T56), E51 (≠ Y65), E162 (= E177), H194 (= H209)
binding magnesium ion: S41 (= S55), E162 (= E177)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 46% coverage: 14:250/514 of query aligns to 1:234/374 of 2awnB

query
sites
2awnB

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

H

-

L

-

P

-

A

L

R

K

F

L

G

A

L

G

V

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

-

R

-

V

-

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P

R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S

S

L

G

G

G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
31% identity, 46% coverage: 14:250/514 of query aligns to 1:234/372 of 2awoA

query
sites
2awoA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

H

-

L

-

P

-

A

L

R

K

F

L

G

A

L

G

V

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

-

R

-

V

-

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P

R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S

S

L

G

G

G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D
|
D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56)
binding magnesium ion: S42 (= S55), D157 (= D176)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puyA

query
sites
3puyA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E
x
P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

-

L

-

K

H

L

L

A

P

G

A

A

R

K

F

K

G

E

L

V

V

I

N

N

R

Q

G

R

V

V

L

-

R

-

Q

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L

L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D
|
D

E
|
E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F25), V17 (≠ A30), P36 (≠ E49), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), Q81 (= Q97), R128 (≠ P145), A132 (≠ V149), S134 (= S153), G135 (≠ L154), G136 (= G155), Q137 (= Q156), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97), D157 (= D176)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puxA

query
sites
3puxA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

-

L

-

K

H

L

L

A

P

G

A

A

R

K

F

K

G

E

L

V

V

I

N

N

R

Q

G

R

V

V

L

-

R

-

Q

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L
|
L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D
|
D

E
|
E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), R128 (≠ P145), A132 (≠ V149), L133 (= L152), S134 (= S153), Q137 (= Q156)
binding beryllium trifluoride ion: S37 (≠ N50), G38 (= G51), K41 (= K54), Q81 (= Q97), S134 (= S153), G135 (≠ L154), G136 (= G155), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97), D157 (= D176)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puwA

query
sites
3puwA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

-

L

-

K

H

L

L

A

P

G

A

A

R

K

F

K

G

E

L

V

V

I

N

N

R

Q

G

R

V

V

L

-

R

-

Q

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L

L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E
|
E

P

P

T

L

S

S

S
x
N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), R128 (≠ P145), A132 (≠ V149), S134 (= S153), Q137 (= Q156)
binding tetrafluoroaluminate ion: S37 (≠ N50), G38 (= G51), K41 (= K54), Q81 (= Q97), S134 (= S153), G135 (≠ L154), G136 (= G155), E158 (= E177), N162 (≠ S181), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 46% coverage: 14:250/514 of query aligns to 2:235/371 of P68187

query
sites
P68187

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F

W

P

G

G

E

V

V

Q

V

A

V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H
x
A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

H

-

L

-

P

-

A

L

R

K

F

L

G

A

L

G

V

A

N
x
K

R

K

G

E

V

V

L

I

R

N

Q

Q

-

R

-
x
V

-

N

Q

Q

A
x
V

L

A

T
x
E

L

V

L

L

K

Q

G

-

L

L

A
|
A

D

H

E

L

I

L

D

D

P

R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S

S

L

G

G
|
G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D
|
D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q
x
R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

A85 (≠ H100) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ N124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (vs. gap) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A132) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ T134) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G155) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D176) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ Q243) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 46% coverage: 16:250/514 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

S

A

F
x
W

P

G

G

E

V

V

Q

V

A
x
V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

T

R

M

M

A

N

F

D

K

T

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

H

-

L

-

P

-

A

L

R

K

F

L

G

A

L

G

V

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

-

R

-

V

-

N

Q

Q

A

V

L

A

T

E

L

V

L

L

K

Q

G

-

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K
|
K

V
x
A

G

-

R

-

L
|
L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E

E

P

P

T

L

S

S

S
x
N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ F25), V15 (≠ A30), S35 (≠ N50), G36 (= G51), C37 (≠ A52), G38 (= G53), K39 (= K54), S40 (= S55), T41 (= T56), R126 (≠ P145), K129 (= K148), A130 (≠ V149), L131 (= L152), S132 (= S153), G133 (≠ L154), G134 (= G155), Q135 (= Q156), N160 (≠ S181), H189 (= H209)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 46% coverage: 14:250/514 of query aligns to 2:235/369 of P19566

query
sites
P19566

G

A

S

S

L

V

R

Q

F

L

N

R

G

N

I

V

G

T

K

K

S

A

F

W

P

G

G

D

V

V

Q

V

A

V

L

S

A

K

N

D

I

I

S

N

F

L

V

D

A

I

H

H

P

D

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

I

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

T

T

R

M

M

A

N

F

D

K

I

G

P

T

P

A

A

D

E

S

-

I

-

A

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H

A

L
|
L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

S

L

F

G

G

H

-

L

-

P

-

A

L

R

K

F

L

G

A

L

G

V

A

N

K

R

K

G

E

V

V

L

M

R

N

Q

Q

Q

R

A

V

L

N

T

Q

L

V

L

A

K

E

G

V

L

L

A

Q

D

L

E

A

I

H

D

L

P

L

Q

E

E

R

K

K

V

P

G

K

R

A

L

L

S

S

L

G

G

G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

R

V

V

I

F

A

L

F

L

D

D

E

E

P
|
P

T

L

S

S

S

N

L

L

S
x
D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

Y

V

V

A

R

Q

T

V

F

G

E

K

N

P

M

L

S

-

E

E

L

L

T

Y

H

H

-

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

L86 (= L101) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P178) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (≠ S183) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
30% identity, 42% coverage: 29:244/514 of query aligns to 21:244/280 of Q5M244

query
sites
Q5M244

Q

R

A

A

L

L

A

F

N

D

I

M

S

T

F

V

V

T

A

I

H

K

P

D

G

G

Q

S

V

Y

H

T

A

A

L

F

M

I

G

G

E

H

N

T

G

G

A

S

G

G

K

K

S

S

T

T

L

I

L

M

K

Q

I

L

L

L

G

N

G

G

A

L

Y

Y

I

L

P

P

S

T

S

S

G

G

D

Q

L

V

Q

K

I

V

G

D

E

D

Q

T

T

I

M

I

A

N

F

S

K

Q

G

S

T

K

A

N

D

K

S

E

I

I

A

K

S

P

-

I

-

R

-

K

-

V

G

G

V

L

A

V

V

F

I

Q

H

F

Q

P

E

E

L

S

H
x
Q

L

L

V

F

P

A

E

E

M

-

T

T

V

V

A

L

E

E

N

D

L

I

F

A

L

F

G

G

H

-

L

-

P

P

A

Q

R

N

F

F

G

G

L

-

V

V

N

S

R

K

G

E

V

E

L

A

R

E

Q

Q

Q

R

A

A

L

L

T

E

L

S

L

L

K

R

-

L

-

V

G

G

L

L

A

S

D

D

E

E

I

L

D

R

P

D

Q

Q

E

N

K

P

V

F

G

D

R

-

L

L

S

S

L

G

G

G

Q

Q

R

M

Q

R

L

R

V

V

E

A

I

I

A

A

K

G

A

I

L

L

S

A

R

M

G

Q

A

P

H

D

V

I

I

L

A

V

F

L

D

D

E
|
E

P

P

T

T

S

A

S

G

L

L

S

D

A

P

R

Q

E

G

I

R

D

K

R

E

L

L

M

M

A

S

I

L

I

F

G

K

R

Q

L

L

R

H

D

L

E

S

G

G

K

I

V

T

V

I

L

V

Y

L

V

V

S

T

H

H

R

L

M

M

E

D

V

V

F

A

R

D

I

Y

C

A

N

T

A

A

V

V

T

N

V

V

F

M

K

E

D

K

G

G

R

R

Y

L

V

V

R

L

T

S

-

G

-

T

-

P

-

K

-

D

-

V

F

F

E

Q

N

K

M

V

S

A

E

F

L

L

T

K

H

E

D

K

Q

Q

L

L

Q97 (≠ H100) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E177) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
31% identity, 42% coverage: 28:245/514 of query aligns to 20:245/615 of 5lilA

query
sites
5lilA

V

V

Q

H

A
x
V

L

L

A

K

N

D

I

I

S

S

F

L

V

S

A

I

H

E

P

R

G

G

Q

D

V

F

H

V

A

A

L

I

M

M

G

G

E

Q

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

K

N

I

I

L

I

G

G

G

C

A

L

Y

D

I

T

P

A

S

T

S

G

G

G

D

S

L

S

Q

K

I

I

-

D

G

G

E

K

Q

E

T

T

M

I

A

E

F

L

K

T

G

N

T

D

-

Q

-

L

A

S

D

D

S

L

I

R

A

S

S

Q

G

K

V

F

A

G

V

F

I

I

H

F

Q
|
Q

E

R

L

Y

H

N

L

L

V

L

P

S

E

S

M

L

T

T

V

A

A

A

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

L

P

P

A

A

R

I

F

Y

G

A

L

G

V

M

N

P

R

Q

G

S

V

Q

L

R

R

L

Q

E

Q

R

A

A

L

K

T

Q

L

L

K

E

-

K

-

L

G

G

L

L

A

G

D

D

E
x
K

I

-

D

-

P

W

Q

Q

E
x
N

K

K

V

P

G

N

R
x
Q

L
|
L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

Q

Q

Q

L

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

R

N

G

G

A

G

H

E

V

I

I

I

A

L

F

A

D
|
D

E
x
Q

P

P

T

T

S

G

S
x
A

L

L

S

D

A

S

R

H

E

S

I

G

D

E

R

N

L

V

M

M

A

E

I

I

I

L

G

R

R

Q

L

L

R

H

D

E

E

E

G

G

K

H

V

T

V

I

L

I

Y

M

V

V

S

T

H
|
H

R

D

M

-

E

K

E

H

V

I

F

A

R

A

I

S

C

A

N

N

A

R

V

I

T

I

V

E

F

I

K

K

D

D

G

G

-

E

-

I

-

S

-

D

-

T

-

Q

-

K

R

R

Y

Q

V

V

R

K

T

S

-

A

F

V

E

K

N

N

M

P

S

S

E

V

L

F

T

S

H

K

D

D

Q

Q

L

L

V

M

binding adenosine-5'-triphosphate: V22 (≠ A30), S42 (≠ N50), G43 (= G51), S44 (≠ A52), G45 (= G53), K46 (= K54), S47 (= S55), T48 (= T56), Q92 (= Q97), K136 (≠ E142), N139 (≠ E147), Q143 (≠ R151), L144 (= L152), S145 (= S153), G146 (≠ L154), G147 (= G155), Q148 (= Q156), Q169 (≠ E177), A173 (≠ S181), H201 (= H209)
binding magnesium ion: S47 (= S55), Q92 (= Q97), D168 (= D176)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
31% identity, 42% coverage: 28:245/514 of query aligns to 20:245/592 of 5lj7A

query
sites
5lj7A

V

V

Q

H

A
x
V

L

L

A

K

N

D

I

I

S

S

F

L

V

S

A

I

H

E

P

R

G

G

Q

D

V

F

H

V

A

A

L

I

M

M

G

G

E

Q

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

K

N

I

I

L

I

G

G

G

C

A

L

Y

D

I

T

P

A

S

T

S

G

G

G

D

S

L

S

Q

K

I

I

-

D

G

G

E

K

Q

E

T

T

M

I

A

E

F

L

K

T

G

N

T

D

-

Q

-

L

A

S

D

D

S

L

I

R

A

S

S

Q

G

K

V

F

A

G

V

F

I

I

H

F

Q
|
Q

E

R

L

Y

H

N

L

L

V

L

P

S

E

S

M

L

T

T

V

A

A

A

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

L

P

P

A

A

R

I

F

Y

G

A

L

G

V

M

N

P

R

Q

G

S

V

Q

L

R

R

L

Q

E

Q

R

A

A

L

K

T

Q

L

L

K

E

-

K

-

L

G

G

L

L

A

G

D

D

E
x
K

I

-

D

-

P

W

Q

Q

E

N

K

K

V

P

G
x
N

R
x
Q

L
|
L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

Q

Q

Q

L

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

R

N

G

G

A

G

H

E

V

I

I

I

A

L

F

A

D
|
D

E
x
Q

P

P

T

T

S

G

S
x
A