SitesBLAST
Comparing Pf6N2E2_5983 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5983 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
66% identity, 90% coverage: 22:263/270 of query aligns to 1:246/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ A27), V7 (= V28), D39 (≠ C60), I63 (= I84), V101 (= V118), A119 (≠ L136), G120 (= G137), Q122 (= Q139), A123 (= A140), N126 (≠ S143), M128 (≠ N145), N129 (= N146), T131 (= T148)
- binding flavin-adenine dinucleotide: Y13 (= Y34), L182 (= L199)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
59% identity, 92% coverage: 22:269/270 of query aligns to 4:255/255 of P38117
- A9 (= A27) binding
- NPFC 39:42 (= NPFC 57:60) binding
- C66 (≠ I84) binding
- 123:134 (vs. 137:148, 83% identical) binding
- D128 (= D142) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ E168) to M: in dbSNP:rs1130426
- R164 (= R178) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (= E179) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 197:219, 87% identical) Recognition loop
- L195 (= L209) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (= KAK 214:216) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (= KAKK 214:217) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
59% identity, 92% coverage: 22:269/270 of query aligns to 4:255/255 of Q9DCW4
- K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
58% identity, 92% coverage: 22:269/270 of query aligns to 1:252/252 of 2a1uB
- binding adenosine monophosphate: A6 (= A27), V7 (= V28), K8 (= K29), C39 (= C60), C63 (≠ I84), V101 (= V118), L119 (= L136), G120 (= G137), Q122 (= Q139), A123 (= A140), D126 (≠ S143), C128 (≠ N145), N129 (= N146), Q130 (= Q147), T131 (= T148)
- binding flavin-adenine dinucleotide: P37 (= P58), L182 (= L199)
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
58% identity, 92% coverage: 22:269/270 of query aligns to 4:255/255 of Q2TBV3
- K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
31% identity, 90% coverage: 22:264/270 of query aligns to 1:257/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ A27), A7 (≠ V28), K8 (= K29), D38 (≠ C60), T61 (≠ S83), M62 (≠ I84), A93 (≠ L113), D94 (≠ T114), T95 (≠ S115), T98 (≠ V118), L102 (= L122), G118 (= G137), Q120 (= Q139), G124 (≠ S143), T126 (≠ N145), A127 (≠ N146), V129 (≠ T148), T223 (≠ N236)
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
36% identity, 78% coverage: 22:231/270 of query aligns to 1:216/264 of P53570
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
32% identity, 84% coverage: 24:250/270 of query aligns to 4:244/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ A27), I8 (≠ V28), K9 (= K29), D39 (≠ C60), M62 (≠ I84), A93 (≠ L113), D94 (≠ T114), T95 (≠ S115), T98 (≠ V118), L102 (= L122), T121 (≠ L136), G122 (= G137), A125 (= A140), G128 (≠ S143), T130 (≠ N145), G131 (≠ N146), V133 (≠ T148)
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
35% identity, 78% coverage: 22:231/270 of query aligns to 1:215/261 of 1o96A
- binding adenosine monophosphate: A6 (= A27), V7 (= V28), K8 (= K29), D38 (≠ C60), V63 (≠ I84), V99 (= V118), A117 (≠ L136), G118 (= G137), Q120 (= Q139), S121 (≠ A140), Y126 (≠ N145), A127 (≠ N146), T129 (= T148)
- binding flavin-adenine dinucleotide: Q120 (= Q139), Q182 (≠ D198)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 77% coverage: 22:230/270 of query aligns to 1:209/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ A27), V7 (= V28), K8 (= K29), Y37 (≠ F59), D38 (≠ C60), Y40 (≠ I62), T60 (≠ S83), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), M101 (≠ V118), C116 (≠ L136), G117 (= G137), Q119 (= Q139), A120 (= A140), G123 (≠ S143), T125 (≠ N145), A126 (≠ N146), V128 (≠ T148)
- binding nicotinamide-adenine-dinucleotide: M61 (≠ I84), G62 (= G85), P63 (= P86), S85 (≠ E108), D86 (≠ S109), G91 (vs. gap)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 77% coverage: 22:230/270 of query aligns to 1:209/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ A27), V7 (= V28), K8 (= K29), Y37 (≠ F59), D38 (≠ C60), Y40 (≠ I62), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), C116 (≠ L136), G117 (= G137), Q119 (= Q139), A120 (= A140), G123 (≠ S143), T125 (≠ N145), A126 (≠ N146), V128 (≠ T148)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
33% identity, 72% coverage: 22:215/270 of query aligns to 1:194/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ A27), I7 (≠ V28), K8 (= K29), D38 (≠ C60), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), A115 (≠ L136), G116 (= G137), A119 (= A140), G122 (≠ S143), T124 (≠ N145), A125 (≠ N146), V127 (≠ T148)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
29% identity, 75% coverage: 22:223/270 of query aligns to 1:202/251 of 5ow0B
- binding adenosine monophosphate: L6 (≠ A27), A7 (≠ V28), K8 (= K29), D39 (≠ C60), Y62 (≠ I84), I95 (≠ A117), A114 (≠ L136), G115 (= G137), Q117 (= Q139), S118 (≠ A140), R123 (≠ N145), G124 (≠ N146), V125 (≠ Q147), V126 (≠ T148), P127 (≠ G149)
- binding flavin-adenine dinucleotide: L37 (vs. gap), Y38 (≠ F59)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
27% identity, 75% coverage: 23:224/270 of query aligns to 3:207/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ A27), K9 (= K29), D39 (≠ C60), M62 (≠ I84), D94 (= D112), V95 (≠ L113), L102 (≠ V118), G118 (= G137), Q120 (= Q139), T121 (≠ A140), T126 (≠ N145), A127 (≠ N146), V129 (≠ T148)
Query Sequence
>Pf6N2E2_5983 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5983
MSINRQVRLGDFFSPESNEESMKVLVAVKRVVDYNVKVRVKADNSGVDLANVKMSMNPFC
EIAVEEAVRLKEKGVATEIVVVSIGPTTAQEQLRTALALGADRAILVESAEDLTSLAVAK
LLKAVVDKEQPSLVILGKQAIDSDNNQTGQMLAALSGYGQGTFASKVEVSGDSVAVTREI
DGGAQTVSLKLPAIVTTDLRLNEPRYASLPNIMKAKKKPLEVLTPDALGVSTASTNKTLK
VEAPAARSAGIKVKSVAELVEKLKNEAKVI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory