SitesBLAST

A9 (= A27) binding
NPFC 39:42 (= NPFC 57:60) binding
C66 (≠ I84) binding
123:134 (vs. 137:148, 83% identical) binding
D128 (= D142) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
T154 (≠ E168) to M: in dbSNP:rs1130426
R164 (= R178) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
E165 (= E179) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
183:205 (vs. 197:219, 87% identical) Recognition loop
L195 (= L209) mutation to A: Severely impaired in complex formation with ACADM.
K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
KAK 200:202 (= KAK 214:216) mutation to RAR: Does not abolish methylation by ETFBKMT.
KAKK 200:203 (= KAKK 214:217) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.

Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
59% identity, 92% coverage: 22:269/270 of query aligns to 4:255/255 of Q9DCW4

query
sites
Q9DCW4

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K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
58% identity, 92% coverage: 22:269/270 of query aligns to 1:252/252 of 2a1uB

query
sites
2a1uB

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binding adenosine monophosphate: A6 (= A27), V7 (= V28), K8 (= K29), N36 (= N57), C39 (= C60), S62 (= S83), C63 (≠ I84), P98 (≠ S115), V101 (= V118), L119 (= L136), G120 (= G137), K121 (= K138), Q122 (= Q139), A123 (= A140), D126 (≠ S143), C128 (≠ N145), N129 (= N146), Q130 (= Q147), T131 (= T148)
binding flavin-adenine dinucleotide: P37 (= P58), F38 (= F59), L182 (= L199)

Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
58% identity, 92% coverage: 22:269/270 of query aligns to 4:255/255 of Q2TBV3

query
sites
Q2TBV3

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K200 (= K214) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K217) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
31% identity, 90% coverage: 22:264/270 of query aligns to 1:257/262 of 6fahB

query
sites
6fahB

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binding flavin-adenine dinucleotide: C6 (≠ A27), A7 (≠ V28), K8 (= K29), D38 (≠ C60), T61 (≠ S83), M62 (≠ I84), A93 (≠ L113), D94 (≠ T114), T95 (≠ S115), T98 (≠ V118), L102 (= L122), A117 (≠ L136), G118 (= G137), Q120 (= Q139), A121 (= A140), G124 (≠ S143), T126 (≠ N145), A127 (≠ N146), Q128 (= Q147), V129 (≠ T148), T223 (≠ N236), V225 (≠ T238)

P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
36% identity, 78% coverage: 22:231/270 of query aligns to 1:216/264 of P53570

query
sites
P53570

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Q
|
Q

A
x
S

I

S

D

D

S

Q

D

A

N
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

A

A

A

S

L

Y

S

L

G

N

Y

W

G

P

Q

H

G

A

T

A

F

V

A

V

S

A

K

D

V

L

E

Q

V

Y

S

K

-

P

G

G

D

D

S

N

V

K

A

A

V

V

T

I

R

R

-

R

E

E

I

L

D

E

G

G

A

M

-

L

Q

Q

T

E

V

V

S

E

L

I

K

N

L

C

P

P

A

A

I

V

V

L

T

T

T

I

D

Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

A

A

S

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

P

P

L

I

E

E

V

E

L

V

T

S

P

L

D

A

A

D

L

I

G

G

V

L

S

S

A6 (= A27) binding
NEWD 36:39 (≠ NPFC 57:60) binding
GVQS 119:122 (≠ GKQA 137:140) binding
YAST 127:130 (≠ NNQT 145:148) binding

7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
32% identity, 84% coverage: 24:250/270 of query aligns to 4:244/282 of 7koeA

query
sites
7koeA

V

V

L

V

V

V

A
x
C

V
x
I

K
|
K

R

Q

V

V

V

P

D

D

Y

-

N

T

V

T

K

N

V

V

R

R

V

I

K

D

A

R

D

K

N

T

S

N

G

N

V

L

D

V

L

R

A

E

N

G

V

V

K

P

M

S

S

I

M

I

N
|
N

P

P

F

D

C
x
D

E

E

I

R

A

A

V

L

E

E

E

L

A

A

V

S

R

Q

L

L

K

K

E

E

K

K

G

F

V

G

A

A

T

T

E

-

I

V

V

Y

V

V

V

I

S
x
T

I
x
M

G

G

P

P

T

P

T

Q

A

A

Q

K

E

E

Q

A

L

L

R

K

T

D

A

A

L

I

A

A

L

F

G

G

A

L

D

D

R

E

A

A

I

V

L

H

V

L

-

S

-

D

E

R

S

T

A

F

E

A

D

G

L
x
A

T
x
D

S
x
T

L

L

A

A

V
x
T

A

T

K

Y

L

T

L
|
L

K

Y

A

W

V

G

V

I

D

K

K

K

E

I

Q

E

P

E

-

R

-

I

-

G

-

K

-

I

S

D

L

L

V

I

I

L

L
x
T

G
|
G

K

K

Q
|
Q

A
|
A

I

V

D
|
D

S
x
G

D

D

N
x
T

N
x
G

Q
|
Q

T
x
V

G

G

Q

P

M

G

L

L

A

A

A

T

L

R

S

F

G

G

Y

Y

G

A

Q

L

G

G

T

A

F

Y

A

V

S

V

K

R

V

I

E

E

-

E

-

I

-

D

V

P

S

E

G

K

D

K

S

E

V

M

A

V

V

I

T

V

R

R

E

R

I

L

D

D

G

Q

G

G

A

F

Q

E

T

K

V

I

S

R

L

L

K

K

L

L

P

P

A

A

I

V

V

L

T

T

T

I

D

T

L

D

R

E

L

L

N

N

E

K

P

P

R

R

Y

Y

A

A

S

D

L

L

P

P

N

N

I

L

M

I

K

R

A

A

K

I

K

R

K

Y

P

E

L

P

E

I

V

V

L

W

T

T

P

H

D

K

A

D

L

L

G

G

V

L

S

D

T

P

-

K

-

C

-

G

-

F

A

G

S

S

T

P

N

T

K

R

T
x
V

L

V

K

S

V

T

E

N

A

I

P

P

A

P

A

A

R

R

S

K

A

G

G

G

binding flavin-adenine dinucleotide: C7 (≠ A27), I8 (≠ V28), K9 (= K29), N36 (= N57), D39 (≠ C60), T61 (≠ S83), M62 (≠ I84), A93 (≠ L113), D94 (≠ T114), T95 (≠ S115), T98 (≠ V118), L102 (= L122), T121 (≠ L136), G122 (= G137), Q124 (= Q139), A125 (= A140), D127 (= D142), G128 (≠ S143), T130 (≠ N145), G131 (≠ N146), Q132 (= Q147), V133 (≠ T148), V232 (≠ T238)

1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
35% identity, 78% coverage: 22:231/270 of query aligns to 1:215/261 of 1o96A

query
sites
1o96A

M

M

K

K

V

I

L

L

V

V

A
|
A

V
|
V

K
|
K

R

Q

V

T

V

A

-

A

-

L

-

E

D

D

Y

F

N

E

V

I

K

R

V

-

R

-

V

-

K

-

A

-

D

D

N

G

S

M

G

D

V

V

D

D

L

E

A

D

N

F

V

M

K

M

M

Y

S

D

M

L

N
|
N

P

E

F
x
W

C
x
D

E

D

I

F

A

S

V

L

E

E

A

A

V

M

R

K

L

I

K

K

E

E

K

S

G

S

-

D

V

T

A

D

T

V

E

E

I

V

V

V

S
|
S

I
x
V

G

G

P

P

T

D

T

R

A

V

Q

D

E

E

Q

S

L

L

R

R

T

K

A

C

L

L

A

A

L

K

G

G

A

A

D

D

R

R

A

A

I

V

L

R

V

V

-

W

-

D

E

D

S

A

A

A

E

E

D

G

L

S

T

D

S

A

L

I

A

V

V
|
V

A

G

K

R

L

I

L
|
L

K

T

A

E

V

V

V

I

D

K

K

K

E

E

Q

A

P

P

S

D

L

M

V

V

I

F

L
x
A

G
|
G

K
x
V

Q
|
Q

A
x
S

I

S

D

D

S

Q

D
x
A

N
x
Y

N
x
A

Q
x
S

T
|
T

G

G

Q

I

M

S

L

V

A

A

A

S

L

Y

S

L

G

N

Y

W

G

P

Q

H

G

A

T

A

F

V

A

V

S

A

K

D

V

L

E

Q

V

Y

S

K

-

P

G

G

D

D

S

N

V

K

A

A

V

V

T

I

R

R

-

R

E

E

I

L

D

E

G

G

A

M

-

L

Q

Q

T

E

V

V

S

E

L

I

K

N

L

C

P

P

A

A

I

V

V

L

T

T

T

I

D
x
Q

L

L

R

G

L

I

N

N

E

K

P

P

R

R

Y

Y

A

A

S

S

L

L

P

R

N

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

P

P

L

I

E

E

V

E

L

V

T

S

P

L

D

A

A

D

L

I

G

G

V

L

S

S

binding adenosine monophosphate: A6 (= A27), V7 (= V28), K8 (= K29), N35 (= N57), D38 (≠ C60), S62 (= S83), V63 (≠ I84), V99 (= V118), L103 (= L122), A117 (≠ L136), G118 (= G137), V119 (≠ K138), Q120 (= Q139), S121 (≠ A140), A125 (≠ D144), Y126 (≠ N145), A127 (≠ N146), S128 (≠ Q147), T129 (= T148)
binding flavin-adenine dinucleotide: W37 (≠ F59), Q120 (= Q139), Q182 (≠ D198)

4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 77% coverage: 22:230/270 of query aligns to 1:209/263 of 4l2iB

query
sites
4l2iB

M

M

K

N

V

I

L

V

V

V

A
x
C

V
|
V

K
|
K

R

Q

V

V

V

P

D

D

Y

-

N

T

V

A

K

E

V

M

R

K

V

I

K

D

A

P

D

V

N

T

S

N

G

N

V

L

D

V

L

R

A

D

N

G

V

V

K

T

M

N

S

I

M

M

N

N

P
|
P

F
x
Y

C
x
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

V

L

R

Q

L

L

K

K

E

D

K

E

G

-

V

L

A

G

T

A

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G
|
G

P
|
P

T

P

T

H

A

A

Q

E

E

S

Q

V

L

L

R

R

T

D

A

C

L

L

A

A

L

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

V
|
V

E
x
S

S
x
D

-
x
R

-

A

-
x
F

-
x
G

A
|
A

E
x
D

D
x
T

L

L

-

A

T
|
T

S

S

L

A

A

A

V
x
M

A

A

K

N

L

T

L

I

K

K

A

H

V

F

V

-

D

-

K

-

E

G

Q

V

P

P

S

D

L

L

V

I

I

L

L
x
C

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

S
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

I

A

A

A

E

L

H

S

L

G

G

Y

L

G

P

Q

Q

G

V

T

T

F

A

A

A

S

L

K

K

V

V

E

Q

V

V

S

K

G

D

D

D

S

T

V

V

A

V

V

V

T

D

R

R

E

D

I

N

D

E

G

Q

G

M

A

S

Q

M

T

T

V

F

S

T

L

M

K

K

L

M

P

P

A

C

I

V

V

V

T

T

T

V

D

-

L

M

R

R

L

S

N

K

E

D

P

L

R

R

Y

F

A

A

S

S

L

I

P

R

N

G

I

K

M

M

K

K

A

A

K

R

K

K

K

A

P

E

L

I

E

P

V

V

L

Y

T

T

P

A

D

A

A

A

L

L

G

E

V

I

binding flavin-adenine dinucleotide: C6 (≠ A27), V7 (= V28), K8 (= K29), P36 (= P58), Y37 (≠ F59), D38 (≠ C60), Y40 (≠ I62), T60 (≠ S83), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), M101 (≠ V118), C116 (≠ L136), G117 (= G137), Q119 (= Q139), A120 (= A140), G123 (≠ S143), D124 (= D144), T125 (≠ N145), A126 (≠ N146), Q127 (= Q147), V128 (≠ T148)
binding nicotinamide-adenine-dinucleotide: M61 (≠ I84), G62 (= G85), P63 (= P86), V84 (= V107), S85 (≠ E108), D86 (≠ S109), R87 (vs. gap), F89 (vs. gap), G90 (vs. gap), G91 (vs. gap), A92 (= A110)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 77% coverage: 22:230/270 of query aligns to 1:209/263 of 4kpuB

query
sites
4kpuB

M

M

K

N

V

I

L

V

V

V

A
x
C

V
|
V

K
|
K

R

Q

V

V

V

P

D

D

Y

-

N

T

V

A

K

E

V

M

R

K

V

I

K

D

A

P

D

V

N

T

S

N

G

N

V

L

D

V

L

R

A

D

N

G

V

V

K

T

M

N

S

I

M

M

N

N

P

P

F
x
Y

C
x
D

E

Q

I
x
Y

A

A

V

L

E

E

E

T

A

A

V

L

R

Q

L

L

K

K

E

D

K

E

G

-

V

L

A

G

T

A

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G

G

P

P

T

P

T

H

A

A

Q

E

E

S

Q

V

L

L

R

R

T

D

A

C

L

L

A

A

L

V

G

G

A

A

D

D

R

E

A

A

I

K

L

L

V

V

E

S

S

D

-

R

-

A

-

F

-

G

A
|
A

E
x
D

D
x
T

L

L

-

A

T
|
T

S

S

L

A

A

A

V
x
M

A

A

K

N

L

T

L

I

K

K

A

H

V

F

V

-

D

-

K

-

E

G

Q

V

P

P

S

D

L

L

V

I

I

L

L
x
C

G
|
G

K

R

Q
|
Q

A
|
A

I

I

D

D

S
x
G

D
|
D

N
x
T

N
x
A

Q
|
Q

T
x
V

G

G

Q

P

M

E

L

I

A

A

A

E

L

H

S

L

G

G

Y

L

G

P

Q

Q

G

V

T

T

F

A

A

A

S

L

K

K

V

V

E

Q

V

V

S

K

G

D

D

D

S

T

V

V

A

V

V

V

T

D

R

R

E

D

I

N

D

E

G

Q

G

M

A

S

Q

M

T

T

V

F

S

T

L

M

K

K

L

M

P

P

A

C

I

V

V

V

T

T

T

V

D

-

L

M

R

R

L

S

N

K

E

D

P

L

R

R

Y

F

A

A

S

S

L

I

P

R

N

G

I

K

M

M

K

K

A

A

K

R

K

K

K

A

P

E

L

I

E

P

V

V

L

Y

T

T

P

A

D

A

A

A

L

L

G

E

V

I

binding flavin-adenine dinucleotide: C6 (≠ A27), V7 (= V28), K8 (= K29), Y37 (≠ F59), D38 (≠ C60), Y40 (≠ I62), T60 (≠ S83), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), M101 (≠ V118), C116 (≠ L136), G117 (= G137), Q119 (= Q139), A120 (= A140), G123 (≠ S143), D124 (= D144), T125 (≠ N145), A126 (≠ N146), Q127 (= Q147), V128 (≠ T148)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 221, 223

5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
33% identity, 72% coverage: 22:215/270 of query aligns to 1:194/260 of 5ol2B

query
sites
5ol2B

M

M

K

N

V

I

L

V

V

V

A
x
C

V
x
I

K
|
K

R

Q

V

V

V

P

D

D

Y

-

N

-

V

-

K

T

V

T

R

E

V

V

K

K

A

L

D

D

-

P

N

N

S

T

G

G

V

T

D

L

L

I

A

R

N

D

-

G

V

V

K

P

M

S

S

I

M

I

N

N

P

P

F

D

C
x
D

E

K

I

A

A

G

V

L

E

E

A

A

V

I

R

K

L

L

K

K

E

E

K

E

G

-

V

M

A

G

T

A

E

H

I

V

V

T

V

V

V

I

S
x
T

I
x
M

G

G

P

P

T

P

T

Q

A

A

Q

D

E

M

Q

A

L

L

R

K

T

E

A

A

L

L

A

A

L

M

G

G

A

A

D

D

R

R

A

G

I

I

L

L

V

L

E

T

S

D

-

R

-

A

-

F

-

A

-

G

A
|
A

E
x
D

D
x
T

L

W

-

A

T
|
T

S

S

L

S

A

A

V
x
L

A

A

K

G

L

A

L

L

K

K

A

N

V

I

V

-

D

-

K

-

E

-

Q

D

P

F

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x
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G

K
x
R

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A

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D

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x
G

D
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D

N
x
T

N
x
A

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Q

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x
V

G
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G

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M

Q

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I

A

A

A

E

L

H

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N

Y

L

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P

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G

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T

F

Y

A

A

S

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K

E

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G

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S

Y

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L

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G

D

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C

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C

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P

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A

C

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N

N

E

T

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M

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A

binding flavin-adenine dinucleotide: C6 (≠ A27), I7 (≠ V28), K8 (= K29), D38 (≠ C60), T60 (≠ S83), M61 (≠ I84), A92 (= A110), D93 (≠ E111), T94 (≠ D112), T97 (= T114), L101 (≠ V118), A115 (≠ L136), G116 (= G137), R117 (≠ K138), Q118 (= Q139), A119 (= A140), G122 (≠ S143), D123 (= D144), T124 (≠ N145), A125 (≠ N146), Q126 (= Q147), V127 (≠ T148), G128 (= G149)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 222

5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
29% identity, 75% coverage: 22:223/270 of query aligns to 1:202/251 of 5ow0B

query
sites
5ow0B

M

M

K

Q

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I

L

V

V

V

A
x
L

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K

R

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D

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Y
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Y

N

E

V

V

K

-

V

-

R

-

V

-

K

-

A

-

D

-

N

-

S

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D

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A

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N

L

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M

N

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P

-

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Y

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-

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-
x
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E

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K

E

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-

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Y

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Q

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R

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x
G

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A

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K

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G

I

V

V

L

T

S

-

I

T

T

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S

L

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E

N

N

E

V

P

P

R

R

Y

I

A

P

S

A

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K

N

A

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I

M

F

K

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A

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L

binding adenosine monophosphate: L6 (≠ A27), A7 (≠ V28), K8 (= K29), D39 (≠ C60), S61 (= S83), Y62 (≠ I84), I95 (≠ A117), L99 (= L121), A114 (≠ L136), G115 (= G137), Q117 (= Q139), S118 (≠ A140), R123 (≠ N145), G124 (≠ N146), V125 (≠ Q147), V126 (≠ T148), P127 (≠ G149)
binding flavin-adenine dinucleotide: Y13 (= Y34), L37 (vs. gap), Y38 (≠ F59), Q117 (= Q139), S119 (≠ I141)

7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
27% identity, 75% coverage: 23:224/270 of query aligns to 3:207/265 of 7qh2B

query
sites
7qh2B

K

K

V

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x
C

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I

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K

R

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V

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K

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E

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A

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R

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K

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G

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M

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D

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D

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x
T

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x
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x
V

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M

A

A

A

E

L

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G

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T

F

N

A

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K

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-

V

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M

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I

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D

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R

D

L

I

N

Y

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Y

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K

N

R

I

K

M

L

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A

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K

K

K

N

P

P

-

E

L

I

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L

T

T

binding flavin-adenine dinucleotide: C7 (≠ A27), I8 (≠ V28), K9 (= K29), D39 (≠ C60), S61 (= S83), M62 (≠ I84), A93 (≠ E111), D94 (= D112), V95 (≠ L113), T98 (= T114), L102 (≠ V118), C117 (≠ L136), G118 (= G137), K119 (= K138), Q120 (= Q139), T121 (≠ A140), G124 (≠ S143), D125 (= D144), T126 (≠ N145), A127 (≠ N146), Q128 (= Q147), V129 (≠ T148)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 226

Query Sequence

>Pf6N2E2_5983 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_5983
MSINRQVRLGDFFSPESNEESMKVLVAVKRVVDYNVKVRVKADNSGVDLANVKMSMNPFC
EIAVEEAVRLKEKGVATEIVVVSIGPTTAQEQLRTALALGADRAILVESAEDLTSLAVAK
LLKAVVDKEQPSLVILGKQAIDSDNNQTGQMLAALSGYGQGTFASKVEVSGDSVAVTREI
DGGAQTVSLKLPAIVTTDLRLNEPRYASLPNIMKAKKKPLEVLTPDALGVSTASTNKTLK
VEAPAARSAGIKVKSVAELVEKLKNEAKVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory