SitesBLAST
Comparing Pf6N2E2_627 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_627 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
49% identity, 82% coverage: 53:319/325 of query aligns to 54:322/334 of 5aovA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding glyoxylic acid: Y74 (≠ V73), A75 (≠ S74), V76 (= V75), G77 (= G76), R241 (= R237), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), F158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (= S176), R181 (≠ H177), A211 (≠ T207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (= I235), A240 (≠ S236), R241 (= R237), H288 (= H285), G290 (= G287)
Sites not aligning to the query:
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
50% identity, 82% coverage: 53:319/325 of query aligns to 53:321/332 of 6biiA
- active site: L99 (= L99), R240 (= R237), D264 (= D261), E269 (= E266), H287 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V75), T103 (= T103), G156 (= G152), F157 (≠ M153), G158 (= G154), R159 (= R155), I160 (= I156), A179 (≠ S176), R180 (≠ H177), S181 (= S178), K183 (= K180), V211 (≠ L208), P212 (= P209), E216 (= E213), T217 (= T214), V238 (≠ I235), A239 (≠ S236), R240 (= R237), D264 (= D261), H287 (= H285), G289 (= G287)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
51% identity, 82% coverage: 53:319/325 of query aligns to 54:322/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
51% identity, 82% coverage: 53:319/325 of query aligns to 54:322/333 of 2dbqA
- active site: L100 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H288 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V75), T104 (= T103), L158 (≠ M153), G159 (= G154), R160 (= R155), I161 (= I156), S180 (= S176), R181 (≠ H177), T182 (≠ S178), A211 (≠ T207), V212 (≠ L208), P213 (= P209), T218 (= T214), I239 (= I235), A240 (≠ S236), R241 (= R237), D265 (= D261), H288 (= H285), G290 (= G287)
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
41% identity, 91% coverage: 18:312/325 of query aligns to 14:320/328 of Q9UBQ7
- VG 83:84 (= VG 75:76) binding substrate
- GRI 162:164 (= GRI 154:156) binding NADP(+)
- RQPR 185:188 (≠ HSPK 177:180) binding NADP(+)
- S217 (≠ P209) binding NADP(+)
- I243 (= I235) binding NADP(+)
- R245 (= R237) binding substrate
- D269 (= D261) binding substrate
- HIGS 293:296 (≠ HMGS 285:288) binding substrate
- G295 (= G287) binding NADP(+)
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
41% identity, 91% coverage: 18:312/325 of query aligns to 10:316/324 of 2gcgA
- active site: L103 (= L99), R241 (= R237), D265 (= D261), E270 (= E266), H289 (= H285)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (vs. gap), S78 (= S74), V79 (= V75), G80 (= G76), R241 (= R237), H289 (= H285)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V75), T107 (= T103), G156 (= G152), G158 (= G154), I160 (= I156), G180 (≠ S176), R181 (≠ H177), R184 (≠ K180), C212 (≠ L208), S213 (≠ P209), T218 (= T214), I239 (= I235), R241 (= R237), D265 (= D261), H289 (= H285), G291 (= G287)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
43% identity, 79% coverage: 57:312/325 of query aligns to 58:306/313 of Q65CJ7
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
43% identity, 79% coverage: 57:312/325 of query aligns to 56:304/311 of 3bazA
- active site: L98 (= L99), R230 (= R237), A251 (= A258), D254 (= D261), E259 (= E266), H277 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V75), G149 (= G152), L150 (≠ M153), G151 (= G154), R152 (= R155), I153 (= I156), S172 (= S176), R173 (≠ H177), S174 (= S178), C201 (≠ L208), P202 (= P209), T207 (= T214), I228 (= I235), G229 (≠ S236), R230 (= R237), D254 (= D261), H277 (= H285), G279 (= G287)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
38% identity, 98% coverage: 5:324/325 of query aligns to 4:316/526 of 3dc2A
Sites not aligning to the query:
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
40% identity, 82% coverage: 55:319/325 of query aligns to 56:326/329 of 4e5mA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), R176 (≠ H177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ I235), C236 (≠ S236), R237 (= R237), H292 (= H285), G294 (= G287)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
38% identity, 98% coverage: 5:324/325 of query aligns to 5:317/525 of 3ddnB
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
40% identity, 82% coverage: 55:319/325 of query aligns to 56:326/332 of 4e5pA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), L100 (= L99), T104 (= T103), G154 (= G154), A155 (≠ R155), I156 (= I156), A175 (≠ S176), R176 (≠ H177), L208 (= L208), P209 (= P209), T214 (= T214), P235 (≠ I235), C236 (≠ S236), R237 (= R237), H292 (= H285)
4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
40% identity, 82% coverage: 55:319/325 of query aligns to 56:326/329 of 4e5kA
- active site: L100 (= L99), R237 (= R237), D261 (= D261), E266 (= E266), H292 (= H285)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V75), G77 (= G76), L100 (= L99), T104 (= T103), G152 (= G152), G154 (= G154), A155 (≠ R155), I156 (= I156), H174 (= H175), E175 (vs. gap), A176 (≠ S176), A207 (≠ T207), L208 (= L208), P209 (= P209), P235 (≠ I235), C236 (≠ S236), R237 (= R237), D261 (= D261), H292 (= H285), G294 (= G287)
- binding sulfite ion: L75 (≠ S74), K76 (≠ V75), G77 (= G76), L100 (= L99), R237 (= R237), H292 (= H285)
Sites not aligning to the query:
6ih6A Phosphite dehydrogenase mutant i151r/p176r/m207a from ralstonia sp. 4506 in complex with non-natural cofactor nicotinamide cytosine dinucleotide
37% identity, 98% coverage: 1:319/325 of query aligns to 1:327/330 of 6ih6A
- binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: T104 (= T103), R151 (≠ I151), G154 (= G154), A155 (≠ R155), V156 (≠ I156), D175 (≠ S176), A207 (≠ T207), V208 (≠ L208), P209 (= P209), T214 (= T214), A235 (≠ I235), C236 (≠ S236), R237 (= R237)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
35% identity, 94% coverage: 2:307/325 of query aligns to 2:298/319 of 5v7nA