SitesBLAST

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
57% identity, 100% coverage: 1:102/102 of query aligns to 1:104/104 of P00099

query
sites
P00099

M
|
M

K
|
K

P
|
P

-
x
Y

-
x
A

I
x
L

L
|
L

I
x
S

A
x
L

L
|
L

A
|
A

L
x
T

T
x
G

A
x
T

A
x
L

Y
x
L

S
x
A

L
x
Q

P
x
G

A
|
A

L
x
W

A
|
A

Q
x
E

D
|
D

G
x
P

P
x
E

T
x
V

L
|
L

F
|
F

K
|
K

S
x
N

K
|
K

P
x
G

C
|
C

A
x
V

A
|
A

C
|
C

H
|
H

S
x
A

I
|
I

D
|
D

T
|
T

K
|
K

V
x
M

V
|
V

G
|
G

P
|
P

A
|
A

L
x
Y

K
|
K

D
|
D

V
|
V

A
|
A

K
|
K

N
x
F

A
|
A

G
|
G

V
x
Q

A
|
A

G
|
G

A
|
A

Q
x
E

D
x
A

L
x
E

L
|
L

A
|
A

K
x
Q

H
x
R

I
|
I

K
|
K

E
x
N

G
|
G

T
x
S

Q
|
Q

G
|
G

N
x
V

W
|
W

G
|
G

P
|
P

M
x
I

P
|
P

M
|
M

P
|
P

A
x
P

N
|
N

P
x
A

V
|
V

T
x
S

E
x
D

E
|
E

A
|
A

K
x
Q

I
x
T

L
|
L

A
|
A

A
x
K

W
|
W

V
|
V

L
|
L

T
x
S

L
x
Q

K
|
K

1:22 (vs. 1:20, 36% identical) signal peptide
23:104 (vs. 21:102, 62% identical) modified: mature protein, Cytochrome c-551
H38 (= H36) binding axial binding residue
M83 (= M81) binding axial binding residue

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
62% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 351cA

query
sites
351cA

Q

E

D

D

G

P

P

E

T

V

L

L

F

F

K

K

S

N

K

K

P

G

C
|
C

A

V

A

A

C
|
C

H
|
H

S

A

I

I

D

D

T

T

K

K

V

M

V

V

G
|
G

P
|
P

A

A

L
x
Y

K

K

D

D

V

V

A

A

K

K

N

F

A

A

G

G

V

Q

A

A

G

G

A

A

Q

E

D

A

L

E

L
|
L

A

A

K

Q

H
x
R

I
|
I

K

K

E

N

G

G

T

S

Q
|
Q

G
|
G

N
x
V

W
|
W

G
|
G

P

P

M
x
I

P
|
P

M
|
M

P

P

A

P

N

N

P

A

V

V

T

S

E

D

E

E

A

A

K

Q

I

T

L

L

A

A

A

K

W

W

V

V

L

L

T

S

L

Q

K

K

binding protoporphyrin ix containing fe: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), Y27 (≠ L47), L44 (= L64), R47 (≠ H67), I48 (= I68), Q53 (= Q73), G54 (= G74), V55 (≠ N75), W56 (= W76), G57 (= G77), I59 (≠ M79), P60 (= P80), M61 (= M81)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
61% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 2exvA

query
sites
2exvA

Q

E

D

D

G

P

P

E

T

V

L

L

F

A

K

K

S

N

K

K

P

G

C
|
C

A

V

A

A

C
|
C

H
|
H

S

A

I

I

D

D

T

T

K

K

V

M

V

V

G
|
G

P
|
P

A

A

L

Y

K

K

D

D

V

V

A

A

K

K

N

F

A

A

G

G

V

Q

A

A

G

G

A

A

Q

E

D

A

L

E

L

L

A

A

K

Q

H
x
R

I
|
I

K

K

E

N

G

G

T
x
S

Q
|
Q

G
|
G

N
x
V

W
|
W

G
|
G

P

P

M
x
I

P
|
P

M
|
M

P

P

A

P

N
|
N

P

A

V

V

T

S

E

D

E

E

A

A

K

Q

I

T

L

L

A

A

A

K

W

W

V

V

L

L

T

S

L

Q

K

K

binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), R47 (≠ H67), I48 (= I68), S52 (≠ T72), Q53 (= Q73), G54 (= G74), V55 (≠ N75), W56 (= W76), G57 (= G77), I59 (≠ M79), P60 (= P80), M61 (= M81), N64 (= N84)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
59% identity, 76% coverage: 25:102/102 of query aligns to 3:80/80 of 5xecC

query
sites
5xecC

T

Q

L

L

F

A

K

K

S

Q

K

K

P

G

C
|
C

A

M

A

A

C
|
C

H
|
H

S

D

I

L

D

K

T

A

K

K

V

M

V

V

G

G

P
|
P

A

A

L
x
Y

K

K

D

D

V

V

A

A

K

K

N

F

A

A

G

G

V

Q

A

A

G

G

A

A

Q

E

D

A

L

E

L
|
L

A

A

K

Q

H
x
R

I

I

K

K

E

N

G

G

T

S

Q
|
Q

G

G

N
x
V

W
|
W

G
|
G

P

P

M
x
I

P

P

M
|
M

P

P

A

P

N
|
N

P

A

V

V

T

S

E

D

E

E

A

A

K

Q

I

T

L

L

A

A

A

K

W

W

V

V

L

L

T

S

L

Q

K

K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P23 (= P45), Y25 (≠ L47), L42 (= L64), R45 (≠ H67), Q51 (= Q73), V53 (≠ N75), W54 (= W76), G55 (= G77), I57 (≠ M79), M59 (= M81), N62 (= N84)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
56% identity, 80% coverage: 21:102/102 of query aligns to 1:82/82 of 5xecA

query
sites
5xecA

Q

E

D

D

G

P

P

E

T

V

L

L

F

F

K

K

S

N

K

K

P

G

C
|
C

A

V

A

A

C
|
C

H
|
H

S

A

I

I

D

D

T

T

K

K

V

K

V

V

G
|
G

P
|
P

A

A

L
x
Y

K

A

D

D

V

V

A

A

A

K

K

K

N
x
Y

A

A

G

G

V

R

A

K

G

D

A

A

Q

V

D

D

L
x
Y

L

L

A

A

K

G

H

K

I
|
I

K

K

E

K

G

G

T

G

Q
x
S

G
|
G

N
x
V

W
|
W

G
|
G

P

S

M
x
V

P

P

M
|
M

P

P

A

P

N
x
Q

P

N

V

V

T

T

E

D

E

A

E

E

A

A

K

K

I

Q

L

L

A

A

A

Q

W

W

V
x
I

L

L

T

S

L

I

K

K

binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), G24 (= G44), P25 (= P45), Y27 (≠ L47), Y34 (≠ N54), Y43 (≠ L63), I48 (= I68), S53 (≠ Q73), G54 (= G74), V55 (≠ N75), W56 (= W76), G57 (= G77), V59 (≠ M79), M61 (= M81), Q64 (≠ N84), I78 (≠ V98)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
55% identity, 75% coverage: 26:102/102 of query aligns to 4:81/81 of 1a56A

query
sites
1a56A

L

L

F

A

K

K

S

K

K

N

P
x
N

C
|
C

A

I

A

A

C
|
C

H
|
H

S

Q

I

V

D

E

T

T

K

K

V

V

V
|
V

G
|
G

P
|
P

A

A

L

L

K

K

D

D

V

I

A

A

K

K

N

Y

A

A

G

D

V

K

A

D

G

D

A

A

Q

A

D

T

L

Y

L

L

A

A

K

G

H
x
K

I
|
I

K

K

E

G

G

G

T
x
S

Q

S

G

G

N

V

W
|
W

G

G

P
x
Q

M
x
I

P

P

M
|
M

P

P

A

P

N
|
N

-
x
V

P
x
N

V

V

T

S

E

D

E

A

E

D

A

A

K

K

I

A

L

L

A

A

A

D

W

W

V

I

L

L

T

T

L

L

K

K

binding heme c: N9 (≠ P31), C10 (= C32), C13 (= C35), H14 (= H36), V21 (= V43), G22 (= G44), P23 (= P45), K45 (≠ H67), I46 (= I68), S50 (≠ T72), W54 (= W76), Q56 (≠ P78), I57 (≠ M79), M59 (= M81), N62 (= N84), V63 (vs. gap), N64 (≠ P85)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
51% identity, 75% coverage: 26:102/102 of query aligns to 4:83/83 of 5aurA

query
sites
5aurA

L

L

F

A

K

K

S

Q

K

K

P

G

C
|
C

A

M

A

A

C
|
C

H
|
H

S

D

I

L

D

K

T

A

-

G

K

K

V

K

V

V

G

G

P
|
P

A

A

L
x
Y

K

A

D

D

V

V

A

A

A

K

K

K

N
x
Y

A

A

G

G

V

R

A

K

G

D

A

A

Q

V

D

D

L
x
Y

L

L

A

A

K

G

H

K

I
|
I

K

K

E

K

G

G

T

G

Q
x
S

G
|
G

N
x
V

W
|
W

G
|
G

P

S

M
x
V

P
|
P

M
|
M

P

P

A

P

N
x
Q

P

N

V

V

T

T

E

D

E

A

E

E

A

A

K

K

I

Q

L

L

A

A

A

Q

W

W

V

I

L

L

T

S

L

I

K

K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P26 (= P45), Y28 (≠ L47), Y35 (≠ N54), Y44 (≠ L63), I49 (= I68), S54 (≠ Q73), G55 (= G74), V56 (≠ N75), W57 (= W76), G58 (= G77), V60 (≠ M79), P61 (= P80), M62 (= M81), Q65 (≠ N84)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
47% identity, 75% coverage: 26:102/102 of query aligns to 4:79/79 of 2d0sA

query
sites
2d0sA

L

L

F

A

K

K

S

A

K

K

P

G

C
|
C

A

M

A

A

C
|
C

H
|
H

S

A

I

I

D

D

T

K

K

K

V

L

V

V

G

G

P
|
P

A

S

L

Y

K

K

D

D

V

V

A

A

A

K

K

K

N
x
Y

A

T

G

E

V

A

A

D

G

V

A

P

Q

K

D

-

L

-

L

L

A

V

K

E

H
x
K

I
x
V

K

K

E

K

G

G

T

G

Q
x
A

G
|
G

N
x
V

W
|
W

G
|
G

P

P

M
x
V

P

P

M
|
M

P
|
P

A

P

N
x
H

P

P

-

Q

V

V

T

A

E

E

E

A

E

D

A

I

K

E

I

K

L

I

A

V

A

R

W

W

V

V

L

L

T

T

L

L

K

K

binding heme c: C10 (= C32), C13 (= C35), H14 (= H36), P23 (= P45), Y32 (≠ N54), K43 (≠ H67), V44 (≠ I68), A49 (≠ Q73), G50 (= G74), V51 (≠ N75), W52 (= W76), G53 (= G77), V55 (≠ M79), M57 (= M81), P58 (= P82), H60 (≠ N84)

3cp5A CytochromE C from rhodothermus marinus (see paper)
31% identity, 79% coverage: 18:98/102 of query aligns to 24:109/116 of 3cp5A

query
sites
3cp5A

A

A

L

L

A

A

Q

Q

D

Q

G

G

P

E

T

Q

L

L

F

F

K

N

S

T

K
x
Y

P

-

C
|
C

A

T

A

A

C
|
C

H
|
H

S

R

I

L

D

D

T

E

K

R

V

F

V

I

G
|
G

P
|
P

A

A

L

L

K

R

D

D

V

V

A

T

A

K

K
x
R

N
x
R

A

G

G

P

V

V

A
x
Y

-

I

-

M

-

N

-
x
V

-
x
M

-

L

G

N

A

P

Q

N

D

G

L

M

L

I

A

Q

K

R

H

H

-

P

-

V

I
x
M

K

K

E

Q

G
x
L

T
x
V

Q

Q

G

-

N

E

W

Y

G

G

P
x
T

M
|
M

P

-

M
|
M

P

T

A

D

N

M

P

A

V

L

T

S

E

E

E

Q

A

A

K

R

I

A

L
x
I

A

L

A

E

W

Y

V

L

binding heme c: Y36 (≠ K30), C37 (= C32), C40 (= C35), H41 (= H36), G49 (= G44), P50 (= P45), R58 (≠ K53), R59 (≠ N54), Y63 (≠ A58), V67 (vs. gap), M68 (vs. gap), M81 (≠ I68), L84 (≠ G71), V85 (≠ T72), T90 (≠ P78), M91 (= M79), M92 (= M81), I105 (≠ L94)

6cunA Engineered cytochromE C from rhodothermus marinus (rma tde) bound to carbene intermediate (1-ethoxy-1-oxopropan-2-ylidene) (see paper)
48% identity, 39% coverage: 18:57/102 of query aligns to 24:62/112 of 6cunA

query
sites
6cunA

A

A

L

L

A

A

Q

Q

D

Q

G

G

P

E

T

Q

L

L

F

F

K

N

S

T

K

Y

P

-

C
|
C

A

T

A

A

C
|
C

H
|
H

S

R

I

L

D

D

T

E

K

R

V

F

V

I

G

G

P
|
P

A

A

L

L

K

R

D

D

V

V

A

T

A

K

K
x
R

N
x
R

A

G

G

P

V

V

binding heme c: C37 (= C32), C40 (= C35), H41 (= H36), P50 (= P45), R58 (≠ K53), R59 (≠ N54)

Sites not aligning to the query:

binding ethyl propionate: 67, 71, 75, 81
binding heme c: 63, 67, 84, 85, 89, 102

3x15A Dimeric aquifex aeolicus cytochrome c555 (see paper)
34% identity, 66% coverage: 22:88/102 of query aligns to 2:58/87 of 3x15A

query
sites
3x15A

D

D

G

G

P

K

T

A

L

I

F

F

K

Q

S

Q

K

K

P

G

C
|
C

A

G

A

S

C
|
C

H
|
H

S

Q

I

A

D

N

T

V

K

D

V

T

V

V

G

G

P
|
P

A

S

L

L

K

K

D

K

V

I

A

A

A

-

K

-

N

-

A

Q

G

A

V
x
Y

A

A

G

G

A

K

Q

E

D

D

L

Q

L

L

A

I

K

K

H
x
F

I

L

K

K

E

-

G

-

T

-

Q

-

G

-

N

-

W

-

G

G

P

E

M

A

P

P

M
x
A

P
x
I

A
x
V

N

D

P

P

V

A

T
x
K

E
|
E

binding heme c: C12 (= C32), C15 (= C35), H16 (= H36), P25 (= P45), Y34 (≠ V57), F44 (≠ H67), A51 (≠ M81), I52 (≠ P82), V53 (≠ A83), K57 (≠ T87), E58 (= E88)

Sites not aligning to the query:

binding heme c: 61, 64

Query Sequence

>Pf6N2E2_704 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_704
MKPILIALALTAAYSLPALAQDGPTLFKSKPCAACHSIDTKVVGPALKDVAAKNAGVAGA
QDLLAKHIKEGTQGNWGPMPMPANPVTEEEAKILAAWVLTLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory