SitesBLAST
Comparing Pf6N2E2_883 Major myo-inositol transporter IolT to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
35% identity, 90% coverage: 44:470/472 of query aligns to 21:437/446 of A0A0H2VG78
- D22 (= D45) mutation to N: Affects symport activity. May function as an uniporter.
- R102 (= R125) mutation to A: Loss of transport activity.
- I105 (≠ L128) mutation to S: Affects symport activity. May function as an uniporter.
- E122 (= E145) mutation to A: Loss of transport activity.
- Q137 (≠ E160) mutation to A: Loss of transport activity.
- Q250 (≠ S282) mutation to A: Loss of transport activity.
- Q251 (= Q283) mutation to A: Loss of transport activity.
- N256 (= N288) mutation to A: Loss of transport activity.
- W357 (= W390) mutation to A: Loss of transport activity.
Q8VZR6 Inositol transporter 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 98% coverage: 9:472/472 of query aligns to 7:485/509 of Q8VZR6
- ER 481:482 (≠ -R 469) mutation to AA: No effect on targeting.
Sites not aligning to the query:
- 479:509 mutation Missing: Leads to endoplasmic reticulum relocalization.
- 500:509 mutation Missing: Leads to endoplasmic reticulum relocalization.
- 502:504 mutation LLE->AAA,SSS: Leads to plasma membrane relocalization.
P0AGF4 D-xylose-proton symporter; D-xylose transporter from Escherichia coli (strain K12) (see paper)
31% identity, 94% coverage: 25:467/472 of query aligns to 7:475/491 of P0AGF4
- F24 (= F42) mutation to A: Decreases xylose transport.
- G83 (= G93) mutation to A: Abolishes xylose transport.
- R133 (= R125) mutation R->C,H,L: Abolishes xylose transport.
- E153 (= E145) mutation to A: Abolishes xylose transport.
- R160 (= R152) mutation to A: Abolishes xylose transport.
- Q168 (≠ E160) binding ; mutation to A: Abolishes xylose transport.
- Q288 (≠ S282) mutation to A: Abolishes xylose transport.
- QQ 288:289 (≠ SQ 282:283) binding
- Q289 (= Q283) mutation to A: Strongly decreases xylose transport.
- N294 (= N288) binding ; mutation to A: Abolishes xylose transport.
- Y298 (= Y292) mutation to A: Abolishes xylose transport.
- N325 (≠ S319) mutation to A: No effect on xylose transport.
- G340 (= G334) mutation to A: Abolishes xylose transport.
- R341 (= R335) mutation R->A,W: Abolishes xylose transport.
- W392 (= W390) binding ; mutation to A: Abolishes xylose transport.
- E397 (= E395) mutation to A: Abolishes xylose transport.
- R404 (= R402) mutation to A: Strongly decreases xylose transport.
- Q415 (≠ F413) binding
- W416 (= W414) mutation to A: Strongly decreases xylose transport.
4gc0A The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to 6-bromo-6-deoxy-d-glucose (see paper)
31% identity, 94% coverage: 25:467/472 of query aligns to 3:471/475 of 4gc0A
4gbzA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-glucose (see paper)
31% identity, 94% coverage: 25:467/472 of query aligns to 3:471/475 of 4gbzA
- binding beta-D-glucopyranose: F20 (= F42), Q164 (≠ E160), Q171 (= Q167), Q284 (≠ S282), Q285 (= Q283), N290 (= N288), L293 (≠ M291), F379 (= F377), G384 (= G386), W388 (= W390), Q411 (≠ F413)
4gbyA The structure of the mfs (major facilitator superfamily) proton:xylose symporter xyle bound to d-xylose (see paper)
31% identity, 94% coverage: 25:467/472 of query aligns to 3:471/475 of 4gbyA
Q9C757 Probable inositol transporter 2 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 72% coverage: 10:350/472 of query aligns to 10:355/580 of Q9C757
Sites not aligning to the query:
- 399 C→A: Strongly decreased nickel inhibition; when associated with A-402, A-410 and A-413.; C→S: No effect on inostol transport or nickel inhibition. No effect on inostol transport or nickel inhibition; when associated with S-410.
- 402 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-410 and A-413.
- 410 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-413.; C→S: No effect on inostol transport or nickel inhibition; when associated with S-399.
- 413 C→A: Strongly decreased nickel inhibition; when associated with A-399, A-402 and A-410.
O23492 Inositol transporter 4; Myo-inositol-proton symporter INT4; Protein INOSITOL TRANSPORTER 4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 70% coverage: 27:356/472 of query aligns to 26:363/582 of O23492
Sites not aligning to the query:
- 559:561 LLE→AAA: No effect on targeting.
- 559:582 mutation Missing: No effect on targeting.
- 564:565 FK→AA: No effect on targeting.
- 570:575 RRREKK→AAAAAA: No effect on targeting.
P39004 High-affinity hexose transporter HXT7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 99% coverage: 3:467/472 of query aligns to 39:525/570 of P39004
Sites not aligning to the query:
- 560 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
P39003 High-affinity hexose transporter HXT6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 99% coverage: 3:467/472 of query aligns to 39:525/570 of P39003
Sites not aligning to the query:
- 560 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q9P3U7 Probable high-affinity hexose transporter ght8, mitochondrial; Hexose transporter 8 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 92% coverage: 36:468/472 of query aligns to 14:465/547 of Q9P3U7