SitesBLAST
Comparing PfGW456L13_1420 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1420 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3q2iA Crystal structure of the wlba dehydrognase from chromobactrium violaceum in complex with nadh and udp-glcnaca at 1.50 a resolution (see paper)
24% identity, 97% coverage: 2:342/351 of query aligns to 9:337/345 of 3q2iA
- active site: K104 (= K95), H192 (= H180)
- binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: K104 (= K95), N133 (≠ R124), T164 (≠ R155), R165 (≠ K156), Y169 (= Y160), N188 (≠ T176), Q189 (≠ N177), H192 (= H180), K247 (≠ R250), N248 (≠ H251)
- binding 1,4-dihydronicotinamide adenine dinucleotide: C19 (≠ A12), G20 (= G13), R21 (≠ N14), I22 (≠ M15), D44 (= D36), I45 (≠ P37), T81 (≠ N72), P82 (= P73), S83 (≠ N74), H86 (= H77), E103 (= E94), K104 (= K95), Q132 (≠ H123), W175 (= W165), R176 (= R166), H192 (= H180)
3q2kC Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
25% identity, 97% coverage: 2:341/351 of query aligns to 5:339/347 of 3q2kC
- active site: K100 (= K95), H188 (= H180)
- binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R17 (≠ N14), K100 (= K95), W159 (≠ L154), T160 (≠ R155), R161 (≠ K156), Y165 (= Y160), N184 (≠ T176), Q185 (≠ N177), H188 (= H180), Q243 (≠ R250), N244 (≠ H251)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G16 (= G13), R17 (≠ N14), I18 (≠ M15), D40 (= D36), T41 (≠ P37), A76 (= A71), T77 (≠ N72), S79 (≠ N74), H82 (= H77), Q85 (≠ T80), E99 (= E94), K100 (= K95), Q128 (≠ H123), W171 (= W165), R172 (= R166)
3q2kK Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
25% identity, 96% coverage: 5:341/351 of query aligns to 3:314/322 of 3q2kK
- active site: K95 (= K95), H183 (= H180)
- binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R12 (≠ N14), K95 (= K95), T155 (≠ R155), R156 (≠ K156), Y160 (= Y160), N179 (≠ T176), Q180 (≠ N177), H183 (= H180), N239 (≠ H251)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G11), G11 (= G13), R12 (≠ N14), I13 (≠ M15), D35 (= D36), T36 (≠ P37), T72 (≠ N72), P73 (= P73), S74 (≠ N74), L76 (= L76), H77 (= H77), E94 (= E94), K95 (= K95), Q123 (≠ H123), W166 (= W165), R167 (= R166), H183 (= H180)
8qc8D Crystal structure of NAD-dependent glycoside hydrolase from flavobacterium sp. (Strain k172) in complex with co-factor NAD+ (see paper)
30% identity, 62% coverage: 5:221/351 of query aligns to 2:225/339 of 8qc8D
- binding nicotinamide-adenine-dinucleotide: G10 (= G13), H11 (≠ N14), M12 (= M15), D33 (= D36), P34 (= P37), T35 (≠ G38), S38 (≠ A41), A71 (= A71), S72 (≠ N72), P73 (= P73), N74 (= N74), H77 (= H77), E96 (= E94), K97 (= K95), E125 (≠ H123), K162 (≠ F161), V163 (≠ E162), W166 (= W165)
8qc8B Crystal structure of NAD-dependent glycoside hydrolase from flavobacterium sp. (Strain k172) in complex with co-factor NAD+ (see paper)
32% identity, 52% coverage: 5:186/351 of query aligns to 6:193/367 of 8qc8B
- binding nicotinamide-adenine-dinucleotide: H15 (≠ N14), M16 (= M15), D37 (= D36), P38 (= P37), T39 (≠ G38), S42 (≠ A41), A75 (= A71), S76 (≠ N72), P77 (= P73), N78 (= N74), H81 (= H77), E100 (= E94), K101 (= K95), E129 (≠ H123), K166 (≠ F161), V167 (≠ E162), W170 (= W165)
Sites not aligning to the query:
8qc2A Crystal structure of NAD-dependent glycoside hydrolase from flavobacterium sp. (Strain k172) in complex with co-factor NAD+ and sulfoquinovose (sq) (see paper)
32% identity, 52% coverage: 5:186/351 of query aligns to 4:191/363 of 8qc2A
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), H13 (≠ N14), M14 (= M15), D35 (= D36), P36 (= P37), T37 (≠ G38), S40 (≠ A41), A73 (= A71), S74 (≠ N72), P75 (= P73), N76 (= N74), H79 (= H77), E98 (= E94), K99 (= K95), E127 (≠ H123), K164 (≠ F161), V165 (≠ E162), W168 (= W165)
- binding sulfoquinovose: K99 (= K95), Y128 (≠ R124), R158 (= R155), F161 (≠ D158), L162 (≠ S159), K164 (≠ F161), E181 (≠ T176), H185 (= H180)
Sites not aligning to the query:
3ceaA Crystal structure of myo-inositol 2-dehydrogenase (np_786804.1) from lactobacillus plantarum at 2.40 a resolution
24% identity, 97% coverage: 3:342/351 of query aligns to 3:336/342 of 3ceaA
- active site: K98 (= K95), H185 (= H180)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), G13 (= G13), R14 (≠ N14), L15 (≠ M15), L38 (≠ D36), Q42 (= Q40), V74 (≠ A71), A75 (≠ N72), P76 (= P73), T77 (≠ N74), F79 (≠ L76), H80 (= H77), M83 (≠ T80), E97 (= E94), K98 (= K95), M127 (≠ H123), F169 (≠ W165), H185 (= H180), F286 (≠ T290)
7x2yA Crystal structure of cis-4,5-dihydrodiol phthalate dehydrogenase in complex with NAD+ and 3-hydroxybenzoate (see paper)
28% identity, 64% coverage: 5:227/351 of query aligns to 4:224/342 of 7x2yA
- binding 3-hydroxybenzoic acid: H122 (= H122), Q173 (≠ N177), H176 (= H180)
- binding nicotinamide-adenine-dinucleotide: G10 (= G11), G12 (= G13), R13 (≠ N14), A14 (≠ M15), D35 (= D36), P36 (= P37), R37 (vs. gap), A71 (= A71), S72 (≠ N72), P73 (= P73), F76 (≠ L76), Q80 (≠ T80), E94 (= E94), K95 (= K95), P96 (= P96), R161 (≠ W165), P163 (≠ R167), E164 (= E168)
Sites not aligning to the query:
8qc6A Crystal structure of NAD-dependent glycoside hydrolase from arthrobacter sp. U41 in complex with NAD+ and sulfoquinovose (sq) (see paper)
31% identity, 52% coverage: 5:186/351 of query aligns to 6:193/366 of 8qc6A
- binding nicotinamide-adenine-dinucleotide: H15 (≠ N14), M16 (= M15), D37 (= D36), P38 (= P37), S42 (≠ A41), A75 (= A71), S76 (≠ N72), P77 (= P73), N78 (= N74), H81 (= H77), E100 (= E94), K101 (= K95), E129 (≠ H123), K166 (≠ F161), V167 (≠ E162), W170 (= W165)
- binding sulfoquinovose: K101 (= K95), Y130 (≠ R124), R160 (= R155), F163 (≠ D158), L164 (≠ S159), K166 (≠ F161), E183 (≠ T176), K184 (≠ N177), H187 (= H180)
Sites not aligning to the query:
1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
24% identity, 95% coverage: 5:338/351 of query aligns to 4:315/325 of 1zh8A
- active site: K98 (= K95), H187 (= H180)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), C11 (≠ A12), G12 (= G13), I13 (≠ N14), A14 (≠ M15), S37 (≠ A35), R38 (≠ D36), T39 (≠ P37), H42 (≠ Q40), T74 (≠ A71), L75 (≠ N72), P76 (= P73), L79 (= L76), E97 (= E94), K98 (= K95), N126 (≠ H123), Y165 (= Y160), W170 (= W165), R171 (= R166), H187 (= H180), Y276 (≠ L300)
1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
25% identity, 86% coverage: 40:342/351 of query aligns to 40:326/340 of 1evjA
- active site: K100 (= K95), Y188 (≠ H180)
- binding nicotinamide-adenine-dinucleotide: L77 (≠ N72), P78 (= P73), N79 (= N74), H82 (= H77), E99 (= E94), K100 (= K95), R128 (≠ H123), W170 (≠ T163), R171 (≠ P164), Y188 (≠ H180), Y267 (≠ W283)
Sites not aligning to the query:
6jw6A The crystal structure of kand2 in complex with NAD (see paper)
32% identity, 63% coverage: 4:224/351 of query aligns to 1:224/341 of 6jw6A
- binding nicotinamide-adenine-dinucleotide: G10 (= G13), F11 (≠ N14), M12 (= M15), D33 (= D36), L34 (≠ P37), T70 (≠ A71), T71 (≠ N72), P72 (= P73), N73 (= N74), L75 (= L76), H76 (= H77), Q79 (≠ T80), E93 (= E94), K94 (= K95), N122 (≠ H123), W161 (≠ F161), H179 (= H180)
Sites not aligning to the query:
6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
32% identity, 63% coverage: 4:224/351 of query aligns to 1:224/342 of 6jw8A
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: F11 (≠ N14), F152 (≠ W152), N154 (≠ L154), D175 (≠ T176), L176 (≠ N177), H179 (= H180)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G13), F11 (≠ N14), M12 (= M15), D33 (= D36), L34 (≠ P37), T70 (≠ A71), T71 (≠ N72), P72 (= P73), N73 (= N74), L75 (= L76), H76 (= H77), Q79 (≠ T80), E93 (= E94), K94 (= K95), N122 (≠ H123), W161 (≠ F161), H179 (= H180)
Sites not aligning to the query:
- binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 290
6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
32% identity, 63% coverage: 4:224/351 of query aligns to 1:224/342 of 6jw7A
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: F11 (≠ N14), N154 (≠ L154), D175 (≠ T176), H179 (= H180)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G11), G10 (= G13), F11 (≠ N14), M12 (= M15), D33 (= D36), L34 (≠ P37), T70 (≠ A71), T71 (≠ N72), P72 (= P73), N73 (= N74), L75 (= L76), H76 (= H77), Q79 (≠ T80), E93 (= E94), K94 (= K95), N122 (≠ H123), W161 (≠ F161)
Sites not aligning to the query:
- binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: 236, 271
- binding 1,4-dihydronicotinamide adenine dinucleotide: 290
6a3iA Levoglucosan dehydrogenase, complex with nadh and levoglucosan (see paper)
27% identity, 64% coverage: 5:229/351 of query aligns to 3:249/372 of 6a3iA
- binding Levoglucosan: K103 (= K95), Y132 (≠ R124), Y160 (≠ W152), Q162 (= Q153), R175 (= R166), D188 (≠ T176), H192 (= H180)
- binding 1,4-dihydronicotinamide adenine dinucleotide: F12 (≠ N14), M13 (= M15), E42 (vs. gap), A43 (vs. gap), P81 (= P73), N82 (= N74), L84 (= L76), H85 (= H77), E102 (= E94), K103 (= K95), W174 (= W165), R175 (= R166)
6a3jC Levoglucosan dehydrogenase, complex with nadh and l-sorbose (see paper)
27% identity, 63% coverage: 5:226/351 of query aligns to 2:250/378 of 6a3jC
- binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G13), F11 (≠ N14), M12 (= M15), E41 (vs. gap), T79 (≠ N72), P80 (= P73), N81 (= N74), H84 (= H77), E101 (= E94), K102 (= K95), W173 (= W165), R174 (= R166)
- binding alpha-L-sorbopyranose: K102 (= K95), Y131 (≠ R124), Y159 (≠ W152), Q161 (= Q153), W163 (≠ R155), R174 (= R166), D187 (≠ T176)
6o15A Crystal structure of a putative oxidoreductase yjhc from escherichia coli in complex with NAD(h) (see paper)
24% identity, 68% coverage: 5:243/351 of query aligns to 2:244/355 of 6o15A
- binding nicotinamide-adenine-dinucleotide: G10 (= G13), Y11 (≠ N14), F12 (≠ M15), Y32 (≠ A35), D33 (= D36), A67 (= A71), T68 (≠ N72), P69 (= P73), N70 (= N74), H73 (= H77), E90 (= E94), K91 (= K95), I119 (≠ H123), W160 (= W165)
1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
25% identity, 86% coverage: 40:342/351 of query aligns to 71:369/383 of 1h6dA
- active site: K131 (= K95), Y219 (≠ H180)
- binding glycerol: K131 (= K95), R202 (≠ P164), D215 (≠ T176), Y219 (≠ H180)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K71 (≠ Q40), Y89 (≠ F53), I107 (≠ A71), L108 (≠ N72), P109 (= P73), N110 (= N74), H113 (= H77), E130 (= E94), K131 (= K95), R159 (≠ H123), A198 (≠ Y160), W201 (≠ T163), R202 (≠ P164), Y219 (≠ H180), Y298 (≠ R250)
Sites not aligning to the query:
1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
25% identity, 86% coverage: 40:342/351 of query aligns to 69:367/381 of 1rydA
- active site: K129 (= K95), Y217 (≠ H180)
- binding alpha-D-glucopyranose: Y236 (≠ V195), I254 (≠ A213), Q256 (≠ L215)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K69 (≠ Q40), Y87 (≠ F53), L106 (≠ N72), P107 (= P73), N108 (= N74), L110 (= L76), H111 (= H77), E128 (= E94), K129 (= K95), R157 (≠ H123), A196 (≠ Y160), W199 (≠ T163), R200 (≠ P164), Y217 (≠ H180), Y296 (≠ R250)
Sites not aligning to the query:
6ktkC Crystal structure of scyllo-inositol dehydrogenase r178a mutant, complexed with nadh and l-glucono-1,5-lactone, from paracoccus laeviglucosivorans (see paper)
31% identity, 64% coverage: 5:229/351 of query aligns to 3:248/368 of 6ktkC
- binding L-glucono-1,5-lactone: K101 (= K95), Y130 (≠ R124), Y158 (≠ W152), E160 (vs. gap), Y162 (vs. gap), D186 (≠ T176), H190 (= H180)
- binding 1,4-dihydronicotinamide adenine dinucleotide: T10 (≠ A12), G11 (= G13), F12 (≠ N14), M13 (= M15), D40 (= D36), M41 (vs. gap), T77 (≠ A71), T78 (≠ N72), N80 (= N74), H83 (= H77), E100 (= E94), K101 (= K95), N129 (≠ H123), H190 (= H180)
Query Sequence
>PfGW456L13_1420 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1420
LNSPLRIALIGAGNMGQQHYQHLQSLTEATLCAVADPGPQAASLAAQWGVACFADHRQML
EQAKPDAVIVANPNTLHVSTALDCLAAGVPVLLEKPVGVHLDEARELVTASKASGVPVLV
GHHRRHNPLIVRAHELVHSGALGRLTTVTALWQLRKPDSYFETPWRREAGAGMLLTNLIH
DLDLLRHLCGEVRQVQAITSNAIRGFANEDCAAVLLQFDNGALGSLTGSDAVAAPWSWEL
DSGENPVYPRHPDQPCYLLAGTGGALSIPQLKRWHYNDVDGGWHQPLLATQENFSADEAL
RLQLQHFVRVARREVEPLVSAADAARTLALIEAIREAAETGRACAPSLIEG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory