SitesBLAST

HLN 66:68 (= HLN 83:85) binding
Y138 (≠ F155) binding
GFA 199:201 (= GFA 216:218) binding
N203 (= N220) binding
R206 (= R223) binding
PL 236:237 (≠ PV 253:254) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
74% identity, 86% coverage: 23:276/297 of query aligns to 1:254/254 of 1fx8A

query
sites
1fx8A

S

T

L

L

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S

G

Q

Q

C

C

M

I

A

A

E

E

F

F

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L

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T

T

A

G

L

L

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I

F

F

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G

G

C

C

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V

A

A

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L

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K

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V

A

A

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G

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A

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F

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Q

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W

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I

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W

G

G

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A

M

M

A

A

I

I

Y

Y

L

L

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T

A

A

G

G

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V

S

S

G

G

A

A

H
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H

L
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L

N
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N

P

P

A

A

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T

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I

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A

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I

L

F

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A

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Y

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L

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x
Y

P
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P
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P

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T

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D

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A

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I

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R

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D

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Y

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P

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C

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H

L

L

binding glycerol: W43 (= W65), V47 (= V69), H61 (= H83), L62 (= L84), N63 (= N85), Y133 (≠ F155), P134 (= P156), N135 (= N157), P136 (= P158), I182 (= I204), G190 (= G212), P191 (= P213), G194 (= G216), G194 (= G216), F195 (= F217), A196 (= A218), N198 (= N220), R201 (= R223)

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
69% identity, 86% coverage: 23:277/297 of query aligns to 4:258/269 of B1VB61

query
sites
B1VB61

S

S

L

L

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A

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C

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A

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E

F

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A

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F

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G

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C

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A

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K

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A

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F

L

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C

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A

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C

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A

A

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S

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L

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M

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M

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A

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D

D

N

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N

L

L

P

P

NPA 66:68 (= NPA 85:87) NPA 1
NPA 201:203 (= NPA 220:222) NPA 2

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
69% identity, 86% coverage: 23:277/297 of query aligns to 4:258/264 of P37451

query
sites
P37451

S

S

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L

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A

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A

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A

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C

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A

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W

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A

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A

A

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A

A

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C

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A

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S

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L

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M

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D

N

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N

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P

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F

A

A

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M

N
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N

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A
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A

R

R

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D

F

F

G

G

P

P

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K

L

L

M

F

T

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F

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A

A

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N

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A

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N

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L

P

P

NPA 66:68 (= NPA 85:87) NPA 1
NPA 201:203 (= NPA 220:222) NPA 2

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
39% identity, 90% coverage: 24:289/297 of query aligns to 20:281/301 of Q96PS8

query
sites
Q96PS8

L

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F

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x
G

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H

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L

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N

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x
F

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A

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D
x
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W

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A

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N

D

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x
N

H

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T

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G

T

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Q

K

A

-

S

-

L

-

E

E

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F

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F

Y

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Y

L

L

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L

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N

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-

V

G

G

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A

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I

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D

D

R

N

N

N

K

G

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L

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M

M

G

G

P

A

L

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C

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N

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P

A

A

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D

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P

P

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M

F

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A

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W

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S

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A

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R

N

D

G

I

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P

-

Y

W

F

W

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W

I

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Q

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L

L

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A

A

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L

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L

H

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K

Q

D

D

A

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T

V

A

S

A

A

E27 (= E31) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ T76) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S80) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H83) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V88) mutation to A: Nearly abolishes permeability to glycerol.
R94 (≠ D97) mutation to A: Abolishes permeability to glycerol.
N133 (≠ H136) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

6f7hD Crystal structure of human aqp10 (see paper)
40% identity, 85% coverage: 24:275/297 of query aligns to 8:255/256 of 6f7hD

query
sites
6f7hD

L

L

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A

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Q

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C

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L

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A

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F

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G

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F

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-

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A

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A

A

H
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H

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L

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N

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P

A

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A

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C

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L

A

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S

A

A

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F

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C

G

A

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L

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N

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Q

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A

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S

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-

E

E

L

T

A

A

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F

F

S

A

T

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F

Y

P

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A

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A

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L
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L

M

I

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V

G

G

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x
L

I

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D

D

R

N

N

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K

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M

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A

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P

P

K

R

L

L

M

F

T

T

F

Y

F

V

A

A

G

G

W

W

G

G

E

P

I

E

S

V

F

F

T

S

G

A

G

G

R

N

D

G

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

V

V

F

V

A

A

P

P

I

L

V

V

G

G

A

A

C

T

L

V

G

G

A

T

A

A

A

T

Y

Y

R

Q

G

L

L

L

I

V

A

A

R

L

H

H

binding glycerol: H68 (= H83), N70 (= N85), L158 (= L176), L162 (≠ V179), N202 (= N220)

O14520 Aquaporin-7; AQP-7; Aquaglyceroporin-7; Aquaporin adipose; AQPap; Aquaporin-7-like from Homo sapiens (Human) (see 4 papers)
32% identity, 94% coverage: 13:291/297 of query aligns to 22:295/342 of O14520

query
sites
O14520

V
x
I

S
x
A

M
x
K

T
x
I

T
x
Q

D
x
E

L
x
I

Q
x
L

Q
|
Q

P
x
R

S
x
K

L

M

S

V

S

R

Q

E

C

F

M

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-
x
V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

I

L

I

G

W

F

G

G

V

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

T

A

A

G

G

R

V

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

S

C

I

A

F

L

A

G

D

R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

F

V

Y

Y

I

V

F

L

A

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

G

A

A

A

L

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S
x
Y

N

T

L

A

F

I

F

L

E

H

F

F

E

S

Q

G

T

G

H

Q

H

L

M

M

V

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

F

Y

P

L

N

P

P

D

V
x
H

L

M

T

T

T

L

A

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M

F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

K

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

A

A

G

G

W

W

G

G

E

K

I

Q

S

V

F

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

V

V

F

V

A

A

P

P

I

L

V

L

G
|
G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

R

L

G

V

L

F

I

I

A

G

R

S

H

T

L

I

P

P

S

R

A

E

V

P

P

L

A

K

T

L

K

E

D

D

A

S

T

V

A

A

A

Y

I

E

D

D

V59 (vs. gap) to L: in dbSNP:rs4008659
Y135 (≠ S126) Important for permeability to glycerol; mutation to A: Strongly decreased permeability to glycerol. Mildly decreased water permeability.
H165 (≠ V159) mutation to A: Decreased permeability to glycerol. Mildly decreased water permeability.
G264 (= G260) to V: affects water and glycerol transport; dbSNP:rs62542743

Sites not aligning to the query:

1:32 mutation Missing: Decreased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

6n1gA Crystal structure of aquaglyceroporin aqp7 (see paper)
34% identity, 83% coverage: 23:268/297 of query aligns to 1:241/249 of 6n1gA

query
sites
6n1gA

S

K

L

M

S

V

S

R

Q

E

C

F

M

L

A

A

E

E

F

F

L

M

G

S

T

T

A

Y

L

V

L

M

I

M

F

V

F

F

G

G

T

L

G

G

C

S

V

V

A

A

A

H

L

M

-

V

-

L

-

N

K

K

V

K

A

Y

G

G

A

S

S

Y

F

L

G

G

L

-

W

-

E

-

I

V

S

N

I

L

I

G

W

F

G

G

V

F

G

G

V

V

S

T

M

M

A

G

I

V

Y

H

L

V

T

A

A

G

G

R

V

I

S

S

G

G

A

A

H

H

L

M

N

N

P

A

A

A

V

V

S

T

I

F

A

A

L

N

S

C

I

A

F
x
L

A
x
G

D
x
R

F

V

E

P

K

W

R

R

K

K

L

F

P

P

F

V

Y

Y

I

V

F

L

A

G

Q

Q

V

F

A

L

G

G

A

S

F

F

C

L

G

A

A

A

L

A

L

T

V

I

Y

Y

T

S

L

L

Y

F

S

Y

N

T

L

A

F

I

F

L

E

H

F

F

E

S

Q

G

T

G

H

Q

H

L

M

M

V

V

R

T

G

G

T

P

Q

V

A

A

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

A

T

T

F

Y

P

L

N

P

P

D

V

H

L

M

T

T

T

L

A

W

Q

R

A

G

F

F

L

L

V

N

E

E

V

A

I

W

I

L

T

T

A

G

I

M

L

L

M

Q

G

L

V

C

I

L

M
x
F

S

A

L

I

T

T

D

D

D

Q

N

E

N

N

G

N

L

P

P

A

K

L

G

P

P

G

L

T

A

E

P

A

L

L

L

V

I

I

G

G

L

I

L

L

I

V

A

V

V

I

I

I

G

G

S

V

S

S

M

L

G

G

P

M

L

N

T

T

G

G

F

Y

A

A

M

I

N

N

P

P

A

S

R

R

D

D

F

L

G

P

P

P

K

R

L

I

M

F

T

T

F

F

F

I

A

A

G

G

W

W

G

G

E

K

I

Q

S

V

F

F

T

S

G

N

G

G

R

E

D

N

I

-

P

-

Y

W

F

W

L

W

I

V

P

P

V

V

F

V

A

A

P

P

I

L

V

L

G

G

A

A

C

Y

L

L

G

G

A

G

A

I

Y

Y

binding glycerol: L73 (≠ F95), G74 (≠ A96), R75 (≠ D97), F156 (≠ M181)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
37% identity, 89% coverage: 2:264/297 of query aligns to 31:292/306 of I1CR68

query
sites
I1CR68

M

M

E

E

K

S

A

S

P

V

D

F

N

T

K

L

K

Y

N

N

E

K

V

A

S

A

M

D

T

E

T

L

D

D

L

T

Q

S

Q

Q

P

R

S

S

L

A

S

F

S

Q

Q

A

C

C

-

H

-

R

-

E

-

F

M

L

A

A

E

E

F

F

L

I

G

G

T

T

A

V

L

I

L

L

I

V

F

L

G

T

T

C

G

G

C

F

V

C

A

A

A

E

L

Q

K

T

V

L

A

H

G

I

A

E

S

E

F

S

G

K

L

S

W

W

E

L

I

T

S

S

I

S

I

F

W

G

-

S

G

G

V

L

G

S

V

V

S

L

M

I

A

G

I

I

Y

C

L

V

T

S

A

G

G

H

V

V

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

S

C

I

I

F

F

A

S

D

G

F

F

E

P

K

I

R

R

K

K

L

V

P

P

F

S

Y

Y

I

I

F

T

A

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

A

G

G

A

A

L

A

L

L

V

L

Y

Y

T

I

L

I

Y

I

S

E

N

P

L

A

F

I

F

V

E

Q

F

F

E

D

Q

G

T

G

H

Q

H

R

M

Y

V

I

R

L

G

G

T

E

Q

K

A

S

S

T

L

-

E

-

L

-

A

A

S

G

V

I

F

F

S

G

T

T

F

Y

P

P

N

P

P

L

V

Y

L

V

T

G

T

I

A

G

Q

S

A

A

F

I

L

A

V

S

E

E

V

I

I

M

I

G

T

T

A

A

I

M

L

L

M

L

G

L

V

V

I

I

M

M

S

-

L

V

T

T

D

G

D

H

N

P

N

N

G

N

L

L

P

P

K

Y

G

K

P

S

L

A

A

Q

P

G

L

A

L

M

I

I

G

A

L

L

L

G

I

I

A

T

V

T

I

I

G

S

S

L

S

C

M

I

G

G

P

Y

L

T

T

S

G

G

F

F

A

S

M

L

N

N

P

P

A

A

R

R

D

D

F

F

G

G

P

P

K

R

L

L

M

F

T

T

F

A

F

I

A

A

G

G

W

W

G

G

E

F

I

D

S

V

F

F

T

K

G

V

G

Y

R
x
H

D

Y

I

-

P

-

Y

Y

F

A

L

L

I

V

P

P

V

M

F

F

A

A

P

P

I

I

V

L

G

G

A

G

C

L

L

V

G

G

H275 (≠ R245) mutation to A: Affects pH sensing; when associated with A-85.

3c02A X-ray structure of the aquaglyceroporin from plasmodium falciparum (see paper)
36% identity, 85% coverage: 23:273/297 of query aligns to 1:236/242 of 3c02A

query
sites
3c02A

S

S

L

Y

S

V

S

R

Q

E

C

F

M

I

A

G

E

E

F

F

L

L

G

G

T

T

A

F

L

V

L
|
L

I

M

F

F

F

L

G

G

T

E

G

G

C

A

V

T

A

A

A

N

L
x
F

K

H

V

T

A

T

G

G

A

L

S

S

F

G

G

D

L
x
W

W

Y

E

K

I

L

S

C

I

L

I

G

W
|
W

G

G

V

L

G

A

V
|
V

S

F

M

F

A

G

I
|
I

Y

L

L

V

T

S

A

A

G

K

V

L

S

S

G
|
G

A
|
A

H
|
H

L
|
L

N
|
N

P

L

A

A

V

V

S

S

I

I

A

G

L
|
L

S

S

I

S

F

I

A

N

D

K

F

F

E

D

K

L

R

K

K

K

L

I

P

P

F

V

Y

Y

I

F

F

F

A

A

Q

Q

V

L

A

L

G

G

A

A

F

F

C

V

G

G

A

T

L

S

L

T

V

V

Y

Y

T

G

L

L

Y

Y

S

-

N

-

L

-

F

-

E

-

F

-

E

-

Q

-

T

-

H

H

H

G

M

F

V

I

R

S

G

N

T

S

Q

K

A

I

S

P

L

-

E

-

L

-

A

Q

S

F

V

A

F

W

S

E

T

T

F
x
S

P

R

N
|
N

P
|
P

V

S

L

I

T

S

T

L

A

T

Q

G

A

A

F

F

L

F

V

N

E

E

V

L

I

I

I

L

T

T

A

G

I

I

L
|
L

M

L

G

L

V

V

I
|
I

M

L

S

V

L

V

T
x
V

D

D

D

E

N

N

N

I

G

-

L

C

P

G

K

K

G

F

P

H

L

I

A

L

P

K

L

L

-

S

L

V

I

V

G

G

L

L

L

I

I
|
I

A

L

V

C

I

I

G

G

S

I

S

T

M

F

G

G

P
x
G

L

N

T
|
T

G
|
G

F
|
F

A
|
A

M

L

N
|
N

P

P

A

S

R
|
R

D

D

F

L

G

G

P

S

K

R

L

F

M

L

T

S

F

L

F

I

A

A

G

-

W

Y

G

G

E

K

I

D

S

T

F

F

T

T

G

-

R

K

D

D

I

N

P

F

Y

Y

F

F

L

W

I

V

P

P

V

L

F

V

A

A

P

P

I

C

V

V

G

G

A

S

C

V

L

V

G

F

A

C

A

Q

A

F

Y

Y

R

D

G

K

L

V

I

I

A

C

binding glycerol: L16 (= L38), F27 (≠ L49), W36 (≠ L58), W43 (= W65), V47 (= V69), I51 (= I73), G59 (= G81), A60 (= A82), H61 (= H83), H61 (= H83), L62 (= L84), N63 (= N85), L70 (= L92), S120 (≠ F155), N122 (= N157), P123 (= P158), L141 (= L176), I145 (= I180), V149 (≠ T184), I170 (= I204), G179 (≠ P213), G179 (≠ P213), T181 (= T215), G182 (= G216), G182 (= G216), F183 (= F217), A184 (= A218), N186 (= N220), R189 (= R223)

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
35% identity, 84% coverage: 23:271/297 of query aligns to 3:243/245 of 2evuA

query
sites
2evuA

S

S

L

L

S

T

S

K

Q

R

C

C

M

I

A

A

E

E

F

F

L

I

G

G

T

T

A

F

L

I

L
|
L

I

V

F

F

G

G

T

A

G

G

C

S

V

-

A
|
A

A

A

L

V

K

T

V

L

A

M

G

I

A
|
A

S
|
S

F

G

G
|
G

L

T

-
x
S

-

P

-

N

-

P

-

F

-
x
N

-
x
I

-
x
G

-
x
I

-

G

-

L

-

G

-

L

-

G

-

D

W
|
W

-

V

E

A

I

I

S

G

I

L

I

A

W

F

G

G

V

F

G

A

V
x
I

S

A

M

A

A

S

I

I

Y

Y

L

A

T

L

A

G

G

N

V

I

S

S

G
|
G

A
x
C

H
|
H

L
x
I

N
|
N

P

P

A

A

V

V

S

T

I

I

A

G

L
|
L

S

W

I

S

F

V

A

K

D

K

F

F

E

P

K

G

R

R

K

E

L

V

P

V

F

P

Y

Y

I

I

F

I

A

A

Q

Q

V

L

A

L

G

G

A

A

F

A

C

F

G

G

A

S

L

-

V

-

Y

-

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

F

F

E

I

F

F

E

L

Q

Q

T

C

H

A

H

G

M

I

V

G

R

A

G

A

T
|
T

Q

V

A

G

S
x
G

L

L

E
x
G

L

-

A

-

S

-

V

-

F

-

S
x
A

T
|
T

F
x
A

P
|
P

N
x
F

P
|
P

V

G

L

I

T

S

T

Y

A

W

Q

Q

A

A

F

M

L

L

V

A

E

E

V

V

I

V

I

G

T

T

A

F

I

L

L
|
L

M

M

G

I

V

T

I
|
I

M

M

S

G

L

I

T
x
A

D

V

D

D

N

E

N

R

G

A

L

-

P

P

K

K

G

G

P

-

L

F

A

A

P

G

L

I

I
|
I

G

G

L

L

L

T

I

V

A

A

V

G

I

I

G

I

S

T

M

L

G

G

P

N

L

I

T

S

G
|
G

F

S

A

S

M

L

N
|
N

P

P

A

A

R
|
R

D

T

F

F

G

G

P

P

K

Y

L

L

M

-

T

-

F

-

A

-

G

-

W

-

G

N

E

D

I

M

S

I

F

F

T

A

G

G

G

T

R

D

D

L

I

W

P

N

Y

Y

F

Y

L

S

I

I

P

Y

V

V

F

I

A

G

P

P

I

I

V

V

G

G

A

A

C

V

L

L

G

A

A

A

A

L

A

T

Y

Y

R

Q

G

Y

L

L

binding glycerol: L18 (= L38), A26 (= A47), A33 (= A54), S34 (= S55), G36 (= G57), S38 (vs. gap), N43 (vs. gap), I44 (vs. gap), I44 (vs. gap), G45 (vs. gap), I46 (vs. gap), W55 (= W59), I66 (≠ V69), G78 (= G81), C79 (≠ A82), H80 (= H83), I81 (≠ L84), N82 (= N85), L89 (= L92), T128 (= T142), G131 (≠ S145), G133 (≠ E147), A134 (≠ S153), T135 (= T154), A136 (≠ F155), P137 (= P156), F138 (≠ N157), P139 (= P158), L157 (= L176), I161 (= I180), A165 (≠ T184), I179 (= I200), G195 (= G216), N199 (= N220), R202 (= R223)

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
30% identity, 97% coverage: 7:293/297 of query aligns to 19:267/271 of P08995

query
sites
P08995

D

K

N

N

K

T

K

S

N

E

E

T

V

I

S

Q

M

R

T

S

T

D

D

S

L

L

Q

V

Q

S

P

V

S

P

L

F

S

L

S

Q

Q

K

C

L

M

V

A

A

E

E

F

A

L

V

G

G

T

T

A

Y

L

F

L

L

I

I

F

F

F

-

G

-

T

A

G

G

C

C

V

-

A

A

A

S

L

L

K

V

V

V

A

N

G

E

A

N

S

Y

F

Y

G

N

L

M

W

I

E

T

-

F

-

P

-

G

I

I

S

A

I

I

I

V

W

W

G

G

V

L

G

V

V

L

S

T

M

V

A

L

I

V

Y

Y

L

T

T

V

A

G

G

H

V

I

S

S

G

G

A

G

H

H

L

F

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

S

A

I

S

F

T

A

R

D

R

F

F

E

P

K

L

R

I

K

Q

L

V

P

P

F

A

Y

Y

I

V

F

V

A

A

Q

Q

V

L

A

L

G

G

A

S

F

I

C

L

G

A

A

S

L

-

V

-

Y

-

T

-

L

-

Y

-

S

G

N

T

L

L

F

R

F

L

E

L

F

F

E

M

Q

G

T

N

H

H

H

D

M

Q

V

F

R

S

G

G

T

-

Q

-

A

-

S

-

L

-

E

-

L

-

A

-

S

-

V

-

F

-

S

-

T

T

F

V

P

P

N

N

P

G

V

-

L

-

T

T

T

N

A

L

Q

Q

A

A

F

F

L

V

V

F

E

E

V

F

I

I

I

M

T

T

A

F

I

F

L

L

M

M

G

F

V

V

I

I

M

C

S

G

L

V

T

A

D

T

D

D

N

N

N

R

G

A

L

V

P

-

K

-

G

G

P

E

L

F

A

A

P

G

L

I

L

A

I

I

G

G

-

S

-

T

-

L

L

L

I

N

A

V

V

I

I

I

G

G

S

-

M

-

G

G

P

P

L

V

T

T

G

G

F

A

A

S

M

M

N

N

P

P

A

A

R

R

D

S

F

L

G

G

P

P

K

A

L

F

M

V

T

H

F

-

A

-

G

-

W

-

G

G

E

E

I

-

S

-

F

Y

T

E

G

G

G

-

R

-

D

-

I

-

P

-

Y

-

F

I

L

W

I

I

P

Y

V

L

F

L

A

A

P

P

I

V

V

V

G

G

A

A

C

I

L

A

G

G

A

A

A

W

A

V

Y

Y

R

N

G

-

L

-

I

I

A

V

R

R

H

Y

L

T

P

D

S

K

A

P

V

L

P

S

A

E

T

T

K

T

D

K

A

S

T

A

A
x
S

A

F

I

L

D

K

G

G

K

R

S262 (≠ A288) modified: Phosphoserine; by CPK

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 82% coverage: 18:261/297 of query aligns to 41:251/298 of Q6Z2T3

query
sites
Q6Z2T3

D

D

L

F

Q

F

Q

P

P

P

S

H

L

L

S

L

S

K

Q

K

C

V

M

V

A

S

E

E

F

V

L

V

G

A

T

T

A

F

L

L

I

V

F

F

F

M

G

T

T

C

G

G

C

A

V

A

A

G

A

I

L

S

K

G

V

S

A

D

G

L

A

S

S

R

F

I

G

S

L

Q

W

L

E

G

I

Q

S

S

I

I

I

A

W

G

G

G

V

L

G

I

V

V

S

T

M

V

A

M

I

I

Y

Y

L

A

T

V

A

G

G

H

V

I

S

S

G

G

A

A

H

H

L

M

N

N

P

P

A

A

V

V

S

T

I

L

A

A

L

F

S

A

I

V

F

F

A

R

D

H

F

F

E

P

K

W

R

I

K

Q

L

V

P

P

F

F

Y

Y

I

W

F

A

A
|
A

Q

Q

V

F

A

T

G

G

A

A

F

I

C

C

G

A

A

S

L

F

L

V

V

L

Y

-

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

E

-

F

-

E

-

Q

-

T

-

H

-

M

-

V

-

R

-

G

-

T

-

Q

K

A

A

S

V

L

I

E

H

L

P

A

V

S

D

V

V

F

I

S

G

T

T

F

-

P

T

N

T

P

P

V

V

L

G

T

P

T

H

A

W

Q

H

A

S

F

L

L

V

V

V

E

E

V

V

I

I

I

V

T

T

A

F

I

N

L

M

M

M

G

F

V

V

I

T

M

L

S

A

L

V

T

A

D

T

D

D

N

T

N

R

G

A

L

V

P

-

K

-

G

G

P

E

L

L

A

A

P

G

L

L

L

A

I

V

G

G

L

S

L

A

I

V

A

C

V

I

I

T

G

S

S

I

S

F

M

A

G

G

P

A

L

I

T

S

G

G

F

G

A

S

M

M

N

N

P

P

A

A

R

R

D

T

F

L

G

G

P

P

K

A

L

L

M

A

T

S

-

N

-

K

F

F

F

D

A

G

G

L

W

W

G

-

E

-

I

-

S

-

F

-

T

-

G

-

R

-

D

-

I

-

P

I

Y

Y

F

F

L

L

I

G

P

P

V

V

F

M

A

G

P

T

I

L

V

S

G

G

A

A

A132 (= A109) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

4nefA X-ray structure of human aquaporin 2 (see paper)
33% identity, 85% coverage: 17:269/297 of query aligns to 1:219/239 of 4nefA

query
sites
4nefA

T

S

D

E

L

L

Q

R

Q

S

P

I

S

A

L

F

S

S

S

R

Q

A

C

V

M

F

A

A

E

E

F

F

L

L

G

A

T

T

A

L

L

L

L

F

I

V

F

F

G

G

T

L

G

G

C

-

V

-

A

S

A

A

L

L

K

N

V

W

A

P

G

Q

A

A

S

L

F

P

G

S

L

V

W

L

E

Q

I

I

S

A

I

M

I

A

W

F

G

G

V

L

G

G

V

I

S

G

M

T

A

L

I

V

Y

Q

L

A

T

L

A

G

G

H

V

I

S

S

G

G

A

A

H

H

L

I

N

N

P

P

A

A

V

V

S

T

I

V

A

A

L
x
C

S

L

I

V

F

G

A

C

D
x
H

F

V

E

S

K

V

R

L

K

R

L

A

P

A

F

F

Y

Y

I

V

F

A

A

A

Q

Q

V

L

A

L

G

G

A

A

F

V

C

A

G

G

A

A

L

A

L

L

V

L

Y

H

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

E

-

F

-

E

E

Q

I

T

T

H

P

H

A

M

D

V

I

R

R

G

G

T

D

Q

L

A

A

S

V

L

N

E

A

L

L

A

S

S

N

V

-

F

-

S

S

T

T

F

-

P

-

N

-

P

-

V

-

L

-

T

T

T

A

A

G

Q

Q

A

A

F

V

L

T

V

V

E

E

V

L

I

F

I

L

T

T

A

L

I

Q

L

L

M

V

G

L

V

C

I

I

M

F

S

A

L

S

T

T

D

D

D

E

N

R

G

G

L

-

P

-

K

E

G

N

P

P

L

G

A

T

P

P

L

A

L

L

-

S

I

I

G

G

L

F

L

S

I

V

A

A

V

-

I

L

G

G

S

H

S

L

M

L

G

G

-

I

P

H

L

Y

T

T

G

G

F

C

A

S

M

M

N

N

P

P

A

A

R

R

D

S

F

L

G

A

P

P

K

A

L

V

M

V

T

T

F

-

A

-

G

-

W

-

G

G

E

K

I

F

S

D

F

-

T

-

G

-

R

-

D

D

I

H

P

W

Y

V

F

F

L

W

I

I

P

-

V

-

F

-

A

G

P

P

I

L

V

V

G

G

A

A

C

I

L

L

G

G

A

S

A

L

Y

Y

R

N

binding zinc ion: C74 (≠ L92), H79 (≠ D97)

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 11 papers)
33% identity, 85% coverage: 18:268/297 of query aligns to 3:219/271 of P41181

query
sites
P41181

D

E

L

L

Q

R

Q

S

P

I

S

A

L

F

S

S

S

R

Q

A

C

V

M

F

A

A

E

E

F

F

L

L

G

A

T

T

A

L

L

L

L

F

I

V

F

F

G

G

T

L

G

G

C

-

V

-

A

S

A

A

L

L

K

N

V

W

A

P

G

Q

A

A

S

L

F

P

G

S

L

V

W

L

E

Q

I

I

S

A

I

M

I

A

W

F

G

G

V

L

G

G

V

I

S

G

M

T

A

L

I

V

Y

Q

L

A

T

L

A

G

G

H

V

I

S

S

G
|
G

A

A

H

H

L

I

N

N

P

P

A

A

V

V

S

T

I

V

A

A

L

C

S

L

I

V

F

G

A

C

D

H

F

V

E

S

K

V

R

L

K

R

L

A

P

A

F

F

Y

Y

I

V

F

A

A

A

Q

Q

V

L

A

L

G

G

A

A

F

V

C

A

G

G

A

A

L

A

L

L

V

L

Y

H

T

-

L

-

Y

-

S

-

N

-

L

-

F

-

E

-

F

-

E

E

Q

I

T

T

H

P

H

A

M

D

V

I

R

R

G

G

T

D

Q

L

A

A

S

V

L

N

E

A

L

L

A

S

S

N

V

-

F

-

S

S

T

T

F

-

P

-

N

-

P

-

V

-

L

-

T

T

T

A

A

G

Q

Q

A

A

F

V

L

T

V

V

E

E

V

L

I

F

I

L

T

T

A

L

I

Q

L

L

M

V

G

L

V

C

I

I

M

F

S

A

L
x
S

T

T

D

D

D

E

N

R

G

G

L

-

P

-

K

E

G

N

P

P

L

G

A

T

P

P

L

A

L

L

-

S

I

I

G

G

L

F

L

S

I

V

A

A

V

-

I

L

G

G

S

H

S

L

M

L

G

G

-

I

P

H

L

Y

T

T

G

G

F

C

A

S

M

M

N

N

P

P

A

A

R
|
R

D

S

F

L

G
x
A

P

P

K

A

L

V

M
x
V

T

T

F

-

A

-

G

-

W

-

G

G

E

K

I

F

S

D

F

-

T

-

G

-

R

-

D

D

I

H

P

W

Y

V

F

F

L

W

I

I

P

-

V

-

F

-

A

G

P

P

I

L

V

V

G

G

A

A

C

I

L

L

G

G

A
x
S

A

L

Y

Y

G64 (= G81) to R: in NDI2; loss of water channel activity; dbSNP:rs104894326
S148 (≠ L183) mutation to A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; mutation to D: Retained in the endoplasmic reticulum.
R187 (= R223) to C: in NDI2; loss of water channel activity; mutant protein does not fold properly; dbSNP:rs104894328
A190 (≠ G226) to T: in NDI2; mutant protein does not fold properly and is not functional; dbSNP:rs104894341
V194 (≠ M230) to I: in dbSNP:rs772051028
S216 (≠ A265) to P: in NDI2; loss of water channel activity; dbSNP:rs104894329

Sites not aligning to the query:

229 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
231 S→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; S→D: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
244 T→A: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.; T→E: No effect on sorting from the ER to the vesicles, redistribution to apical membrane, or endocytosis.
254 R → L: in NDI2; results in the loss of arginine vasopressin-mediated phosphorylation at S-256; R → Q: in NDI2; exerts a dominant-negative effect on wild-type-AQP2 in that it interferes with its trafficking to the apical membrane; is a loss of function instead of a gain of function mutation on dominant nephrogenic diabetes insipidus
256 modified: Phosphoserine; by PKA; S→A: Retained in vesicles.; S→D: Expressed in the apical membrane.
258 E → K: in NDI2; retained in the Golgi compartment; dbSNP:rs104894332
262 P → L: in NDI2; mutant protein folds properly and is functional but is retained in intracellular vesicles; able to assemble into tetramers with wild-type AQP2 that properly localize to the apical membrane; dbSNP:rs104894339; P→A: No effect on expression at the apical cell membrane.

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 86% coverage: 15:268/297 of query aligns to 67:285/305 of Q9SAI4

query
sites
Q9SAI4

M

V

T

T

T

C

D

S

L

L

Q

P

P

P

-

N

-

V

S

S

L

L

S

Y

S

R

Q

K

C

L

M

G

A

A

E

E

F

F

L

V

G

G

T

T

A

L

L

I

L

L

I

I

F

F

F

A

G

G

T

T

G

A

C

T

V

A

A

I

A

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K

Q

V

K

A

T

G

D

A

G

S

A

F

E

G

T

L

L

W

I

E

G

I

C

S

A

I

A

I

S

W
x
A

G

G

V

L

G

A

V

V

S

M

M

I

A

V

I

I

Y

L

L

S

T

T

A

G

G

H

V

I

S

S

G

G

A

A

H

H

L

L

N

N

P

P

A

A

V

V

S

T

I

I

A

A

L

F

S

A

I

A

F

L

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K

D

H

F

F

E

P

K

W

R

K

K

H

L

V

P

P

F

V

Y

Y

I

I

F

G

A

A

Q

Q

V

V

A

M

G

A

A

S

F

V

C

S

G

A

A

A

L

F

L

A

V

L

Y

K

T

A

L

V

Y

-

S

-

N

-

L

-

F

-

E

-

F

F

E

E

Q

P

T

T

H

-

M

M

V

S

R

G

G

G

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-

L

-

E

-

L

-

A

-

S

-

V

-

F

-

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T

-

F

-

P

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N

-

P

P

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L

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G

T

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Q

A

A

F

F

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A

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F

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L

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M

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F

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M

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S

A

L

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T

A

D

T

D

D

N

T

N

R

G

A

L

V

P

-

K

-

G

G

P

E

L

L

A

A

P

G

L

I

L

A

I

V

G

G

L

A

L

T

I

V

A

M

V

L

I

N

G

I

S

L

S

I

M

A

G

G

P

P

L

A

T

T

G

S

F

A

A

S

M

M

N

N

P

P

A
x
V

R

R

D

T

F

L

G

G

P

P

K

A

L

-

M

-

T

-

F

-

A

-

G

-

W

-

G

-

E

-

I

I

S

A

F

A

T

N

G

N

G

Y

R

R

D

A

I

I

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W

Y

V

F

Y

L

L

I

T

P

-

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-

F

-

A

A

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A

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Y

A119 (≠ W65) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.
V252 (≠ A222) mutation to A: No effect.

P56402 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Mus musculus (Mouse) (see 2 papers)
32% identity, 86% coverage: 18:272/297 of query aligns to 3:223/271 of P56402

query
sites
P56402

D

E

L

L

Q

R

Q

S

P

I

S

A

L

F

S

S

S

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Q

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A

E

E

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A

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T

A

L

L

L

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-

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A

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A

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Q

L

A

T

L

A

G

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H

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S

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A

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Y

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C

A

G

G

A

A

L

A

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V

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Y

H

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-

L

-

Y

-

S

-

N

-

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-

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-

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T
|
T

T

A

A

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Q

Q

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A

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T

T

D

D

-

E

-

R

-

S

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D

N

N

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L

G

G

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P

P

K

A

G

-

P

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-

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-

V

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M

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-

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M

M

N

N

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P

A

A

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-

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W

-

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L

T126 (= T161) mutation to M: Does not cause loss of water channel activity, but impairs trafficking from cytoplasmic vesicles to the cell membrane.

Sites not aligning to the query:

256 modified: Phosphoserine; S → L: in cph; loss of a phosphorylation site and loss of trafficking to the apical cell membrane; causes aberrant location at the basolateral cell membrane

O94778 Aquaporin-8; AQP-8 from Homo sapiens (Human) (see 4 papers)
29% identity, 74% coverage: 10:230/297 of query aligns to 25:220/261 of O94778

query
sites
O94778

K

R

N

W

E

R

V

V

S

S

M

W

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Y

T

E

D

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F

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-

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-

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C
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C

M

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A

V

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L

L

G

G

T

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A

A

L

L

L
x
F

I

I

F

F

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I

G

G

T

-

G

-

C
|
C

V

L

A

S

A

V

L

I

K

E

V

-

A

N

G

G

A

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S

D

F

T

G

G

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L

W

L

E

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S

A

I

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I

A

W
x
H

G

G

V

L

G

A

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S

G

M

L

A

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I

I

Y

A

L

T

T

L

A

G

G

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V

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S

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G

A

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H

L

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N

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P

A

A

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S

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A

A

L

A

S

M

I

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F

I

A

G

D

G

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N