SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_1794 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1794 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
32% identity, 98% coverage: 3:534/545 of query aligns to 7:517/539 of 6lpiB

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active site: I27 (= I23), G29 (= G25), G30 (≠ V26), S31 (≠ H27), I32 (≠ T28), E53 (= E48), C76 (≠ I71), F115 (≠ L112), Q116 (≠ H113), E117 (= E114), K165 (≠ L163), M256 (≠ T251), A283 (≠ D280), V375 (≠ S383), G401 (= G410), M403 (≠ L412), D428 (= D442), N455 (= N469), A457 (≠ G471), L458 (≠ Y472), L460 (≠ E474), V461 (≠ I475), Q464 (≠ Y478)
binding flavin-adenine dinucleotide: R155 (= R153), G212 (= G209), G213 (= G210), G214 (= G211), T236 (= T233), L237 (≠ I234), M238 (≠ N235), L254 (vs. gap), M256 (≠ T251), H257 (≠ Q252), G276 (= G271), A277 (≠ T272), R278 (≠ E273), D280 (≠ T277), R282 (≠ Y279), A283 (≠ D280), D300 (= D297), I301 (= I298), D319 (= D316), V320 (≠ S317), M380 (≠ Y388), G398 (≠ T407)
binding magnesium ion: D428 (= D442), N455 (= N469)
binding thiamine diphosphate: E53 (= E48), C76 (≠ I71), P79 (= P74), G376 (≠ T384), Q377 (= Q385), H378 (≠ P386), G401 (= G410), M403 (≠ L412), G427 (= G441), D428 (= D442), G429 (= G443), S430 (≠ G444), M433 (≠ F447), N455 (= N469), A457 (≠ G471), L458 (≠ Y472), G459 (≠ E473), L460 (≠ E474), V461 (≠ I475)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 16:554/582 of 7tzzA

query
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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ S250), R292 (≠ T282), W489 (vs. gap)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ D382), G401 (≠ S383), Q402 (≠ T384), H403 (≠ Q385), G426 (= G410), M428 (≠ L412), G452 (= G441), D453 (= D442), G454 (= G443), S455 (≠ G444), L483 (≠ Y472), G484 (≠ E473), M485 (vs. gap), V486 (vs. gap)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G209), G223 (= G210), G224 (= G211), T246 (= T233), L247 (≠ I234), M248 (≠ N235), M263 (≠ L247), L264 (≠ I248), M266 (≠ S250), H267 (≠ T251), G286 (= G271), R288 (≠ E273), V293 (≠ F283), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), M405 (≠ V387), G423 (≠ T407)
binding magnesium ion: A37 (≠ H27), T82 (≠ I71), S83 (≠ T72), Q122 (≠ H113), Y381 (≠ F366), D453 (= D442), M458 (≠ F447), Q461 (≠ P450), N480 (= N469), H482 (≠ G471), K533 (≠ V513)

Sites not aligning to the query:

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 568

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 15:553/582 of 3ea4A

query
sites
3ea4A

E

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M

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active site: Y32 (≠ I23), G34 (= G25), G35 (≠ V26), A36 (≠ H27), S37 (≠ T28), E58 (= E48), T81 (≠ I71), F120 (≠ L112), Q121 (≠ H113), E122 (= E114), K170 (≠ L163), M265 (≠ S250), V292 (≠ F283), V399 (≠ D382), G425 (= G410), M427 (≠ L412), D452 (= D442), N479 (= N469), H481 (≠ G471), L482 (≠ Y472), M484 (vs. gap), V485 (vs. gap), W488 (vs. gap)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ V281), R291 (≠ T282), W488 (vs. gap)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G209), G222 (= G210), G223 (= G211), T245 (= T233), L246 (≠ I234), M247 (≠ N235), L263 (≠ I248), G264 (= G249), M265 (≠ S250), H266 (≠ T251), G285 (= G271), R287 (≠ E273), D289 (= D280), R291 (≠ T282), D309 (= D297), I310 (= I298), G327 (≠ A315), D328 (= D316), V329 (≠ S317), M404 (≠ V387), G422 (≠ T407)
binding magnesium ion: D452 (= D442), N479 (= N469), H481 (≠ G471)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ D382), G400 (≠ S383), Q401 (≠ T384), H402 (≠ Q385), M427 (≠ L412), G451 (= G441), D452 (= D442), G453 (= G443), S454 (≠ G444), N479 (= N469), H481 (≠ G471), L482 (≠ Y472), G483 (≠ E473), M484 (vs. gap), V485 (vs. gap)

Sites not aligning to the query:

active site: 557
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 15:553/582 of 3e9yA

query
sites
3e9yA

E

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G

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K

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E

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active site: Y32 (≠ I23), G34 (= G25), G35 (≠ V26), A36 (≠ H27), S37 (≠ T28), E58 (= E48), T81 (≠ I71), F120 (≠ L112), Q121 (≠ H113), E122 (= E114), K170 (≠ L163), M265 (≠ S250), V292 (≠ F283), V399 (≠ D382), G425 (= G410), M427 (≠ L412), D452 (= D442), N479 (= N469), H481 (≠ G471), L482 (≠ Y472), M484 (vs. gap), V485 (vs. gap), W488 (vs. gap)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ V281), R291 (≠ T282), W488 (vs. gap)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G209), G222 (= G210), G223 (= G211), T245 (= T233), L246 (≠ I234), M247 (≠ N235), L263 (≠ I248), G285 (= G271), R287 (≠ E273), D289 (= D280), R291 (≠ T282), D309 (= D297), I310 (= I298), G327 (≠ A315), D328 (= D316), V329 (≠ S317), M404 (≠ V387), G422 (≠ T407)
binding magnesium ion: D452 (= D442), N479 (= N469), H481 (≠ G471)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ D382), G400 (≠ S383), Q401 (≠ T384), H402 (≠ Q385), M427 (≠ L412), G451 (= G441), G453 (= G443), S454 (≠ G444), N479 (= N469), H481 (≠ G471), L482 (≠ Y472), G483 (≠ E473), M484 (vs. gap), V485 (vs. gap)

Sites not aligning to the query:

active site: 557
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 567

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 16:554/585 of 5k2oA

query
sites
5k2oA

E

D

V

I

L

L

V

V

K

E

L

A

L

L

E

E

N

R

Y

Q

G

G

V

V

E

E

Q

T

V

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
S

V

M

E

E

L

I

Y

H

R

Q

G

A

L

L

A

T

R

R

S

S

S

S

-

S

I

I

N

R

H

N

V

V

T

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

T

S

S

S

G

G

K

K

P

P

G

G

V

I

C

C

F

I

I

A

I
x
T

T

S

G

G

P

P

G

G

M

A

T

T

N

N

I

L

T

V

T

S

A

G

M

L

G

A

Q

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

L

V

V

A

I

I

S

T

S

G

V

Q

Q

V

S

P

R

R

S

R

Q

M

L

I

G

-

G

-

G

G

R

T

G

D

K

A

L
x
F

H
x
Q

E
|
E

L

T

P

P

N

I

Q

V

G

-

A

E

L

V

C

T

A

R

G

S

V

I

A

T

A

K

F

H

S

N

H

Y

T

L

L

V

M

M

S

D

A

V

S

E

E

D

L

I

P

P

G

R

V

I

L

I

A

E

R

E

A

A

F

F

A

F

L

L

F

A

Q

T

A

S

G

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

E

D

I

V

P

P

L
x
K

D

D

V

I

L

Q

V

Q

E

Q

E

L

A

A

D

I

-

P

-

N

-

W

D

E

L

Q

L

A

A

M

S

R

L

L

P

P

V

G

N

Y

I

M

D

S

R

R

A

M

G

P

A

K

S

P

P

P

-

E

-

D

S

S

A

H

V

L

S

E

R

Q

M

I

A

V

E

R

L

L

L

I

A

S

G

E

A

S

K

K

R

K

P

P

L

V

I

L

L

Y

A

V

G
|
G

G
|
G

G
|
G

A

C

I

L

D

N

A

S

A

S

V

D

E

E

L

L

T

G

E

R

L

F

A

V

E

E

L

L

L

T

D

G

A

I

P

P

V

V

A

A

L

S

T
|
T

I
x
L

N
x
M

A

G

K

L

G

G

M

S

L

Y

A

P

S

C

G

D

H

D

P

E

L

L

-

S

-

L

-

H

L

M

I
x
L

G

G

S
x
M

T

H

Q

G

S

T

L

V

V

Y

A

A

T

N

R

Y

A

A

L

-

V

V

A

E

E

H

A

S

D

D

V

L

V

L

L

L

A

A

I

F

G
|
G

T

V

E
x
R

L

-

A

-

E

-

T

-

D

-

Y

F

D
|
D

V

D

T
x
R

F
x
V

A

T

G

G

-

K

-

L

-

E

G

A

F

F

E

A

I

S

P

R

G

A

K

K

L

I

L

V

R

H

V

I

D
|
D

I
|
I

D

D

P

S

D

A

Q

E

T

I

V

G

R

K

N

N

Y

K

P

T

P

P

H

H

V

V

A

S

L

V

V

C

A

G

D
|
D

S
x
V

R

K

N

L

A

A

A

L

Q

Q

A

G

L

M

L

N

S

K

A

V

L

L

S

E

H

N

K

R

S

A

L

-

A

E

E

E

R

L

R

K

N

L

D

D

W

F

G

G

Q

V

V

W

R

R

A

N

A

E

R

L

L

N

R

V

E

Q

D

K

L

Q

A

K

A

F

T

P

W

L

D

S

A

F

P

K

T

T

L

F

A

G

Q

E

T

A

-

I

-

P

-

P

R

Q

F

Y

L

A

E

I

T

K

V

V

L

L

Q

D

E

E

L

L

P

T

D

D

A

G

-

K

-

A

-

I

V

I

F

S

V

T

G

G

D
x
V

S
x
G

T
x
Q

Q
x
H

P
x
Q

V
x
M

Y

W

T

A

G

A

N

Q

L

F

T

-

F

Y

N

N

P

Y

E

K

R

K

P

P

R

R

R

Q

W

W

F

L

N

-

S

S

S

S

T
x
G

G

G

Y

L

G
|
G

T

A

L
x
M

G

G

Y

F

A

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

W

S

L

V

G

-

G

-

R

-

I

-

E

-

G

-

G

-

A

A

R

N

P

P

P

D

-

A

-

I

V

V

V

V

C

D

L

I

I

D

G

G

D
|
D

G
|
G

G
x
S

L

F

Q

I

F

M

T

N

L

V

P

Q

E

E

L

L

A

A

S

T

A

I

V

R

E

V

A

E

R

N

T

L

P

P

V

V

I

K

V

V

L

L

L

L

W

L

N

N

N
|
N

Q

Q

G
x
H

Y
x
L

E
x
G

-
x
M

-
x
V

-

M

-

Q

-
x
W

E

E

I

D

K

R

K

F

Y

Y

M

K

V

A

N

N

R

R

A

A

I

H

-

T

-

F

-

L

-

G

E

D

P

P

V

A

G

Q

V

E

D

D

I

E

Y

I

T

F

P

P

D

N

F

M

I

L

G

L

V

F

A

A

K

A

A

A

L

C

G

G

C

I

A

P

A

A

E

A

A

R

V

V

S

T

S

K

V

K

E

A

Q

D

L

L

R

R

G

E

A

A

L

I

R

Q

V

T

A

M

T

L

D

D

R

T

Q

P

G

G

P

P

T

Y

L

L

I

L

E

D

I

V

active site: Y33 (≠ I23), G35 (= G25), G36 (≠ V26), A37 (≠ H27), S38 (≠ T28), E59 (= E48), T82 (≠ I71), F121 (≠ L112), Q122 (≠ H113), E123 (= E114), K171 (≠ L163), M266 (≠ S250), V293 (≠ F283), V400 (≠ D382), G426 (= G410), M428 (≠ L412), D453 (= D442), N480 (= N469), H482 (≠ G471), L483 (≠ Y472), M485 (vs. gap), V486 (vs. gap), W489 (vs. gap)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ S250), R292 (≠ T282), W489 (vs. gap)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G209), G223 (= G210), G224 (= G211), T246 (= T233), L247 (≠ I234), M248 (≠ N235), L264 (≠ I248), G286 (= G271), R288 (≠ E273), D290 (= D280), V293 (≠ F283), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), Q404 (≠ P386), M405 (≠ V387), G423 (≠ T407)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ G471)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ D382), G401 (≠ S383), Q402 (≠ T384), H403 (≠ Q385), M428 (≠ L412), D453 (= D442), G454 (= G443), S455 (≠ G444), N480 (= N469), H482 (≠ G471), L483 (≠ Y472), G484 (≠ E473), M485 (vs. gap), V486 (vs. gap)

Sites not aligning to the query:

active site: 558
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: 568

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 16:554/583 of 5k3sA

query
sites
5k3sA

E

D

V

I

L

L

V

V

K

E

L

A

L

L

E

E

N

R

Y

Q

G

G

V

V

E

E

Q

T

V

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
S

V

M

E

E

L

I

Y

H

R

Q

G

A

L

L

A

T

R

R

S

S

S

S

-

S

I

I

N

R

H

N

V

V

T

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

G

V

F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

T

S

S

S

G

G

K

K

P

P

G

G

V

I

C

C

F

I

I

A

I
x
T

T

S

G

G

P

P

G

G

M

A

T

T

N

N

I

L

T

V

T

S

A

G

M

L

G

A

Q

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

L

V

V

A

I

I

S

T

S

G

V

Q

Q

V

S

P

R

R

S

R

Q

M

L

I

G

-

G

-

G

G

R

T

G

D

K

A

L
x
F

H
x
Q

E
|
E

L

T

P

P

N

I

Q

V

G

-

A

E

L

V

C

T

A

R

G

S

V

I

A

T

A

K

F

H

S

N

H

Y

T

L

L

V

M

M

S

D

A

V

S

E

E

D

L

I

P

P

G

R

V

I

L

I

A

E

R

E

A

A

F

F

A

F

L

L

F

A

Q

T

A

S

G

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

E

D

I

V

P

P

L
x
K

D

D

V

I

L

Q

V

Q

E

Q

E

L

A

A

D

I

-

P

-

N

-

W

D

E

L

Q

L

A

A

M

S

R

L

L

P

P

V

G

N

Y

I

M

D

S

R

R

A

M

G

P

A

K

S

P

P

P

-

E

-

D

S

S

A

H

V

L

S

E

R

Q

M

I

A

V

E

R

L

L

L

I

A

S

G

E

A

S

K

K

R

K

P

P

L

V

I

L

L

Y

A

V

G
|
G

G
|
G

G
|
G

A

C

I

L

D

N

A

S

A

S

V

D

E

E

L

L

T

G

E

R

L

F

A

V

E

E

L

L

L

T

D

G

A

I

P

P

V

V

A

A

L

S

T
|
T

I
x
L

N
x
M

A

G

K

L

G

G

M

S

L

Y

A

P

S

C

G

D

H

D

P

E

L

L

-

S

-

L

-

H

L

M

I
x
L

G

G

S
x
M

T

H

Q

G

S

T

L

V

V

Y

A

A

T

N

R

Y

A

A

L

-

V

V

A

E

E

H

A

S

D

D

V

L

V

L

L

L

A

A

I

F

G
|
G

T

V

E
x
R

L

-

A

-

E

-

T

-

D

-

Y

F

D
|
D

V

D

T
x
R

F
x
V

A

T

G

G

-

K

-

L

-

E

G

A

F

F

E

A

I

S

P

R

G

A

K

K

L

I

L

V

R

H

V

I

D
|
D

I
|
I

D

D

P

S

D

A

Q

E

T

I

V

G

R

K

N

N

Y

K

P

T

P

P

H

H

V

V

A

S

L

V

V

C

A

G

D
|
D

S
x
V

R

K

N

L

A

A

A

L

Q

Q

A

G

L

M

L

N

S

K

A

V

L

L

S

E

H

N

K

R

S

A

L

-

A

E

E

E

R

L

R

K

N

L

D

D

W

F

G

G

Q

V

V

W

R

R

A

N

A

E

R

L

L

N

R

V

E

Q

D

K

L

Q

A

K

A

F

T

P

W

L

D

S

A

F

P

K

T

T

L

F

A

G

Q

E

T

A

-

I

-

P

-

P

R

Q

F

Y

L

A

E

I

T

K

V

V

L

L

Q

D

E

E

L

L

P

T

D

D

A

G

-

K

-

A

-

I

V

I

F

S

V

T

G

G

D
x
V

S
x
G

T
x
Q

Q
x
H

P

Q

V
x
M

Y

W

T

A

G

A

N

Q

L

F

T

-

F

Y

N

N

P

Y

E

K

R

K

P

P

R

R

R

Q

W

W

F

L

N

-

S

S

S

S

T
x
G

G

G

Y

L

G
|
G

T

A

L
x
M

G

G

Y

F

A

G

L

L

P

P

A

A

A

A

I

I

G

G

A

A

W

S

L

V

G

-

G

-

R

-

I

-

E

-

G

-

G

-

A

A

R

N

P

P

P

D

-

A

-

I

V

V

V

V

C

D

L

I

I

D

G

G

D
|
D

G
|
G

G
x
S

L

F

Q

I

F

M

T

N

L

V

P

Q

E

E

L

L

A

A

S

T

A

I

V

R

E

V

A

E

R

N

T

L

P

P

V

V

I

K

V

V

L

L

L

L

W

L

N

N

N
|
N

Q

Q

G
x
H

Y
x
L

E
x
G

-
x
M

-
x
V

-

M

-

Q

-
x
W

E

E

I

D

K

R

K

F

Y

Y

M

K

V

A

N

N

R

R

A

A

I

H

-

T

-

F

-

L

-

G

E

D

P

P

V

A

G

Q

V

E

D

D

I

E

Y

I

T

F

P

P

D

N

F

M

I

L

G

L

V

F

A

A

K

A

A

A

L

C

G

G

C

I

A

P

A

A

E

A

A

R

V

V

S

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S

K

V

K

E

A

Q

D

L

L

R

R

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E

A

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active site: Y33 (≠ I23), G35 (= G25), G36 (≠ V26), A37 (≠ H27), S38 (≠ T28), E59 (= E48), T82 (≠ I71), F121 (≠ L112), Q122 (≠ H113), E123 (= E114), K171 (≠ L163), M266 (≠ S250), V293 (≠ F283), V400 (≠ D382), G426 (= G410), M428 (≠ L412), D453 (= D442), N480 (= N469), H482 (≠ G471), L483 (≠ Y472), M485 (vs. gap), V486 (vs. gap), W489 (vs. gap)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (≠ T282), M485 (vs. gap), W489 (vs. gap)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G209), G223 (= G210), G224 (= G211), T246 (= T233), L247 (≠ I234), M248 (≠ N235), L264 (≠ I248), M266 (≠ S250), G286 (= G271), R288 (≠ E273), D290 (= D280), V293 (≠ F283), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), M405 (≠ V387), G423 (≠ T407)
binding magnesium ion: D453 (= D442), N480 (= N469), H482 (≠ G471)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ D382), G401 (≠ S383), Q402 (≠ T384), H403 (≠ Q385), G426 (= G410), M428 (≠ L412), D453 (= D442), G454 (= G443), S455 (≠ G444), N480 (= N469), H482 (≠ G471), L483 (≠ Y472), G484 (≠ E473), M485 (vs. gap), V486 (vs. gap)

Sites not aligning to the query:

active site: 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
31% identity, 97% coverage: 6:534/545 of query aligns to 16:554/582 of 8et4A

query
sites
8et4A

E

D

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N

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G

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ D382), G401 (≠ S383), Q402 (≠ T384), H403 (≠ Q385), G426 (= G410), M428 (≠ L412), G452 (= G441), D453 (= D442), G454 (= G443), S455 (≠ G444), M458 (≠ F447), N480 (= N469), H482 (≠ G471), L483 (≠ Y472), G484 (≠ E473), M485 (vs. gap), V486 (vs. gap)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G209), G223 (= G210), G224 (= G211), T246 (= T233), L247 (≠ I234), M248 (≠ N235), L264 (≠ I248), M266 (≠ S250), H267 (≠ T251), G286 (= G271), V287 (≠ T272), R288 (≠ E273), D290 (= D280), R292 (≠ T282), V293 (≠ F283), D310 (= D297), I311 (= I298), D329 (= D316), V330 (≠ S317), M405 (≠ V387), G423 (≠ T407)
binding magnesium ion: F370 (≠ A358), D453 (= D442), M458 (≠ F447), Q461 (≠ P450), N480 (= N469), H482 (≠ G471), K533 (≠ V513)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ S250), R292 (≠ T282), M485 (vs. gap),