SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_1873 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P08308 Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
89% identity, 100% coverage: 1:336/336 of query aligns to 1:336/336 of P08308

query
sites
P08308

M
|
M

A

A

F

F

N

N

I

M

H

H

N

N

R

R

N

N

L

L

S

S

L

L

E

M

H

H

T

S

P

T

R

R

E

E

L

L

R

R

Y

Y

L

L

D

D

L

L

S

S

R

R

D

D

L

L

K

K

R

R

A

A

K

K

Y

Y

T

T

G

G

T

T

E

E

Q

Q

H

H

L

L

K

K

G

R

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

T

S

S

T

T

R

R

T

T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

Y

D

D

Q

Q

G

G

A

A

N

N

V

V

T

T

Y

Y

I

I

D

D

P

P

N

N

S

S

Q

Q

I

I

G

G

H

H

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

A

I

I

E
|
E

Y

Y

R

R

G

G

F

F

K

K

Q

Q

E

E

I

I

V

V

E

E

L

L

A

A

K

K

F

F

A

A

G

G

V

V

P

P

V

V

F

F

N

N

G

G

L

L

T

T

D

D

E

E

Y

Y

H

H

P

P

T

T

Q

Q

M

M

I

L

A

A

D

D

V

V

L

L

T

T

M

M

R

R

E

E

H

H

A

S

D

D

K

K

P

P

I

L

H

H

E

D

I

I

S

S

Y

Y

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

M

M

G

G

N

N

S

S

L

L

I

I

G

G

A

A

K

K

L

L

G

G

M

M

D

D

V

V

R

R

I

I

C

A

A

A

P

P

K

K

A

A

L

L

W

W

P

P

A

H

D

D

D

E

L

F

V

V

A

A

R

Q

C

C

K

K

Q

K

Y

F

G

A

E

E

S

S

G

G

A

A

R

K

I

L

T

T

L

L

T

T

E

E

D

D

P

P

K

K

A

E

A

A

V

V

K

K

G

G

V

V

D

D

F

F

I

V

H

H

T

T

D

D

V

V

W

W

V

V

S

S

M

M

G

G

E

E

P

P

V

V

E

E

A

A

W

W

A

G

E

E

R

R

I

I

Q

K

Q

E

L

L

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

E

L

I

M

M

K

K

A

A

T

T

G

G

N

N

P

P

R

R

T

A

K

K

F

F

M

M

H

H

C

C

L

L

P

P

A

A

F

F

H

H

N

N

C

S

D

E

T

T

K

K

V

V

G

G

K

K

Q

Q

I

I

A

A

E

E

Q

Q

Y

Y

P

P

Y

N

L

L

S

A

N

N

G

G

I

I

E

E

V

V

T

T

D

E

D

D

V

V

F

F

E

E

S

S

P

P

A

Y

C

N

I

I

A

A

F

F

E

E

Q

Q

A

A

E

E

N

N

R

R

M

M

H

H

T

T

I

I

K

K

A

A

I

I

L

L

V

V

S

S

T

T

L

L

A

A

D

D

L

I

M1 (= M1) modified: Initiator methionine, Removed
E106 (= E106) mutation E->A,G: Loss of homotropic cooperativity; gain of anabolic activity. Conformational change which modifies the catalytic site. This mutant is blocked in the active R (relaxed) state.

4jqoA Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with citrulline and inorganic phosphate
63% identity, 99% coverage: 1:334/336 of query aligns to 5:337/338 of 4jqoA

query
sites
4jqoA

M

M

A

A

F

F

N

N

I

L

H

R

N

N

R

R

N

N

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

S

P

T

R

K

E

E

L

I

R

Q

Y

F

L

L

L

I

D

D

L

L

S

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

T

A

G

G

T

T

E

E

Q

Q

Q

K

H

K

L

L

K

L

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

A

S

S

T

T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

I

I

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H
x
D

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

F

K

G

Q

Q

E

A

I

I

V

V

E

E

L

L

A

G

K

A

F

F

A

A

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

L

L

T

T

M

M

R

L

E

E

H

H

A

S

D

Q

-

G

K

K

P

A

I

L

H

A

E

D

I

I

S

Q

Y

F

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

N
|
N

M

V

G

G

N

N

S

S

L

L

L

M

L

V

I

G

G

A

A

A

K

K

L

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

A

G

P

P

K

Q

A

A

L

Y

W

W

P

P

A

D

D

E

L

L

V

V

A

A

R

A

C

C

K

Q

Q

A

Y

I

G

A

E

K

E

Q

S

T

G

G

A

G

R

K

I

I

T

T

L

L

T

T

E

E

D

N

P

V

K

A

A

E

A

G

V

V

K

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

V

P

E

E

A

A

W

W

A

D

E

E

R

R

I

V

Q

A

Q

L

L

M

L

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

K

K

A

Q

T

T

G

G

N

N

P

P

R

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

N

C

D

D

E

T

T

K

T

V

I

G

G

K

K

Q

Q

I

V

A

A

E

D

Q

K

Y

F

P

G

Y

M

L

-

S

-

N

K

G

G

I

L

E

E

V

V

T

T

D

E

V

V

F

F

E

E

S

S

P

E

A

H

C

S

I

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

G

active site: R63 (= R59), T64 (= T60), D91 (≠ H87), R112 (= R108), H139 (= H135), Q142 (= Q138), D237 (= D232), C279 (= C274), R324 (= R321)
binding citrulline: H139 (= H135), Q142 (= Q138), N173 (= N168), D237 (= D232), S241 (= S236), M242 (= M237), C279 (= C274), L280 (= L275), R324 (= R321)

4jhxA Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoylphosphate and arginine
63% identity, 99% coverage: 1:334/336 of query aligns to 3:335/336 of 4jhxA

query
sites
4jhxA

M

M

A

A

F

F

N

N

I

L

H

R

N

N

R

R

N

N

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

S

P

T

R

K

E

E

L

I

R

Q

Y

F

L

L

L

I

D

D

L

L

S

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

T

A

G

G

T

T

E

E

Q

Q

Q

K

H

K

L

L

K

L

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

I

I

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H
x
D

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

F

K

G

Q

Q

E

A

I

I

V

V

E

E

L

L

A

G

K

A

F

F

A

A

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L
|
L

T

T

D

D

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

L

L

T

T

M

M

R

L

E

E

H

H

A

S

D

Q

-

G

K

K

P

A

I

L

H

A

E

D

I

I

S

Q

Y

F

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

N
|
N

M

V

G

G

N

N

S

S

L

L

L

M

L

V

I

G

G

A

A

A

K

K

L

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

A

G

P

P

K

Q

A

A

L

Y

W

W

P

P

A

D

D

E

L

L

V

V

A

A

R

A

C

C

K

Q

Q

A

Y

I

G

A

E

K

E

Q

S

T

G

G

A

G

R

K

I

I

T

T

L

L

T

T

E

E

D

N

P

V

K

A

A

E

A

G

V

V

K

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

V

P

E

E

A

A

W

W

A

D

E

E

R

R

I

V

Q

A

Q

L

L

M

L

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

K

K

A

Q

T

T

G

G

N

N

P

P

R

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P
|
P

A

A

F

F

H

H

N

N

C

D

D

E

T

T

K

T

V

I

G

G

K

K

Q

Q

I

V

A

A

E

D

Q

K

Y

F

P

G

Y

M

L

-

S

-

N

K

G

G

I

L

E

E

V

V

T

T

D

E

V

V

F

F

E

E

S

S

P

E

A

H

C

S

I

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

G

active site: R61 (= R59), T62 (= T60), D89 (≠ H87), R110 (= R108), H137 (= H135), Q140 (= Q138), D235 (= D232), C277 (= C274), R322 (= R321)
binding arginine: L132 (= L130), N171 (= N168), D235 (= D232), S239 (= S236), M240 (= M237), P279 (= P276)
binding phosphoric acid mono(formamide)ester: S59 (= S57), T60 (= T58), R61 (= R59), T62 (= T60), R110 (= R108), H137 (= H135), C277 (= C274), L278 (= L275), R322 (= R321)

Q8DCF5 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Vibrio vulnificus (strain CMCP6)
63% identity, 99% coverage: 1:334/336 of query aligns to 1:333/334 of Q8DCF5

query
sites
Q8DCF5

M

M

A

A

F

F

N

N

I

L

H

R

N

N

R

R

N

N

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

S

P

T

R

K

E

E

L

I

R

Q

Y

F

L

L

L

I

D

D

L

L

S

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

T

A

G

G

T

T

E

E

Q

Q

Q

K

H

K

L

L

K

L

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

I

I

D

G

P

P

N

S

S

G

S

S

Q
|
Q

I

I

G

G

H

D

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

F

K

G

Q

Q

E

A

I

I

V

V

E

E

L

L

A

G

K

A

F

F

A

A

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

F

H
|
H

P
|
P

T
|
T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

L

L

T

T

M

M

R

L

E

E

H

H

A

S

D

Q

-

G

K

K

P

A

I

L

H

A

E

D

I

I

S

Q

Y

F

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

N
|
N

M

V

G

G

N

N

S

S

L

L

L

M

L

V

I

G

G

A

A

A

K

K

L

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

A

G

P

P

K

Q

A

A

L

Y

W

W

P

P

A

D

D

E

L

L

V

V

A

A

R

A

C

C

K

Q

Q

A

Y

I

G

A

E

K

E

Q

S

T

G

G

A

G

R

K

I

I

T

T

L

L

T

T

E

E

D

N

P

V

K

A

A

E

A

G

V

V

K

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

V

P

E

E

A

A

W

W

A

D

E

E

R

R

I

V

Q

A

Q

L

L

M

L

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

K

K

A

Q

T

T

G

G

N

N

P

P

R

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

N

C

D

D

E

T

T

K

T

V

I

G

G

K

K

Q

Q

I

V

A

A

E

D

Q

K

Y

F

P

G

Y

M

L

-

S

-

N

K

G

G

I

L

E

E

V

V

T

T

D

E

V

V

F

F

E

E

S

S

P

E

A

H

C

S

I

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

G

STRT 57:60 (= STRT 57:60) binding carbamoyl phosphate
Q84 (= Q84) binding carbamoyl phosphate
R108 (= R108) binding carbamoyl phosphate
HPTQ 135:138 (= HPTQ 135:138) binding carbamoyl phosphate
N169 (= N168) binding L-ornithine
D233 (= D232) binding L-ornithine
SM 237:238 (= SM 236:237) binding L-ornithine
CL 275:276 (= CL 274:275) binding carbamoyl phosphate
R320 (= R321) binding carbamoyl phosphate

4h31A Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoyl phosphate and l-norvaline
63% identity, 99% coverage: 1:334/336 of query aligns to 3:335/335 of 4h31A

query
sites
4h31A

M

M

A

A

F

F

N

N

I

L

H

R

N

N

R

R

N

N

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

S

P

T

R

K

E

E

L

I

R

Q

Y

F

L

L

L

I

D

D

L

L

S

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

T

A

G

G

T

T

E

E

Q

Q

Q

K

H

K

L

L

K

L

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

I

I

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H
x
D

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

F

K

G

Q

Q

E

A

I

I

V

V

E

E

L

L

A

G

K

A

F

F

A

A

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L
|
L

T

T

D

D

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

L

L

T

T

M

M

R

L

E

E

H

H

A

S

D

Q

-

G

K

K

P

A

I

L

H

A

E

D

I

I

S

Q

Y

F

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

N
|
N

M

V

G

G

N

N

S

S

L

L

L

M

L

V

I

G

G

A

A

A

K

K

L

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

A

G

P

P

K

Q

A

A

L

Y

W

W

P

P

A

D

D

E

L

L

V

V

A

A

R

A

C

C

K

Q

Q

A

Y

I

G

A

E

K

E

Q

S

T

G

G

A

G

R

K

I

I

T

T

L

L

T

T

E

E

D

N

P

V

K

A

A

E

A

G

V

V

K

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

V

P

E

E

A

A

W

W

A

D

E

E

R

R

I

V

Q

A

Q

L

L

M

L

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

K

K

A

Q

T

T

G

G

N

N

P

P

R

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

N

C

D

D

E

T

T

K

T

V

I

G

G

K

K

Q

Q

I

V

A

A

E

D

Q

K

Y

F

P

G

Y

M

L

-

S

-

N

K

G

G

I

L

E

E

V

V

T

T

D

E

V

V

F

F

E

E

S

S

P

E

A

H

C

S

I

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

G

active site: R61 (= R59), T62 (= T60), D89 (≠ H87), R110 (= R108), H137 (= H135), Q140 (= Q138), D235 (= D232), C277 (= C274), R322 (= R321)
binding phosphoric acid mono(formamide)ester: S59 (= S57), T60 (= T58), R61 (= R59), T62 (= T60), R110 (= R108), H137 (= H135), Q140 (= Q138), C277 (= C274), L278 (= L275), R322 (= R321)
binding norvaline: L132 (= L130), N171 (= N168), D235 (= D232), S239 (= S236), M240 (= M237)

4jfrB Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoyl phosphate
63% identity, 99% coverage: 1:334/336 of query aligns to 7:339/340 of 4jfrB

query
sites
4jfrB

M

M

A

A

F

F

N

N

I

L

H

R

N

N

R

R

N

N

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

S

P

T

R

K

E

E

L

I

R

Q

Y

F

L

L

L

I

D

D

L

L

S

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

T

A

G

G

T

T

E

E

Q

Q

Q

K

H

K

L

L

K

L

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

Y

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

I

I

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H
x
D

K

K

E

E

S

S

M

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

F

K

G

Q

Q

E

A

I

I

V

V

E

E

L

L

A

G

K

A

F

F

A

A

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

L

L

T

T

M

M

R

L

E

E

H

H

A

S

D

Q

-

G

K

K

P

A

I

L

H

A

E

D

I

I

S

Q

Y

F

A

A

Y

Y

L

L

G

G

D

D

A

A

R

R

N

N

M

V

G

G

N

N

S

S

L

L

L

M

L

V

I

G

G

A

A

A

K

K

L

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

A

G

P

P

K

Q

A

A

L

Y

W

W

P

P

A

D

D

E

L

L

V

V

A

A

R

A

C

C

K

Q

Q

A

Y

I

G

A

E

K

E

Q

S

T

G

G

A

G

R

K

I

I

T

T

L

L

T

T

E

E

D

N

P

V

K

A

A

E

A

G

V

V

K

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S

S

M

M

G

G

E

E

P

S

V

P

E

E

A

A

W

W

A

D

E

E

R

R

I

V

Q

A

Q

L

L

M

L

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

K

K

A

Q

T

T

G

G

N

N

P

P

R

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L

L

P

P

A

A

F

F

H

H

N

N

C

D

D

E

T

T

K

T

V

I

G

G

K

K

Q

Q

I

V

A

A

E

D

Q

K

Y

F

P

G

Y

M

L

-

S

-

N

K

G

G

I

L

E

E

V

V

T

T

D

E

V

V

F

F

E

E

S

S

P

E

A

H

C

S

I

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

G

active site: R65 (= R59), T66 (= T60), D93 (≠ H87), R114 (= R108), H141 (= H135), Q144 (= Q138), D239 (= D232), C281 (= C274), R326 (= R321)
binding phosphoric acid mono(formamide)ester: S63 (= S57), T64 (= T58), R65 (= R59), T66 (= T60), R114 (= R108), H141 (= H135), Q144 (= Q138), C281 (= C274), R326 (= R321)

P04391 Ornithine carbamoyltransferase subunit I; OTCase-1; EC 2.1.3.3 from Escherichia coli (strain K12) (see 7 papers)
60% identity, 99% coverage: 4:334/336 of query aligns to 3:333/334 of P04391

query
sites
P04391

N

G

I

F

H

Y

N

H

R

K

N

H

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

T

P

P

R

A

E

E

L

L

R

N

Y

S

L

L

D

Q

L

L

S

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

T

S

G

G

T

K

E

E

Q

E

Q

A

H

K

L

L

K

T

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

Y

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

I

L

D

G

P

P

N

S

S

G

S

S

Q
|
Q

I

I

G

G

H

H

K
|
K

E

E

S

S

M

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

Y

K

G

Q

Q

E

E

I

I

V

V

E

E

E

T

L

L

A

A

K

E

F

Y

A

A

G

S

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

N

E

E

Y

F

H
|
H

P
|
P

T
|
T

Q
|
Q

M

L

I

L

A

A

D

D

V

L

L

L

T

T

M

M

R

Q

E

E

H

H

-

L

A

P

D

G

K

K

P

A

I

F

H

N

E

E

I

M

S

T

Y

L

A

V

Y

Y

L

A

G

G

D

D

A

A

R

R

N

N

N
|
N

M

M

G

G

N

N

S

S

L

M

L

L

L

E

I

A

G

A

A

A

K

L

L

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

A

A

P

P

K

Q

A

A

L

C

W

W

P

P

A

E

D

A

L

L

V

V

A

T

R

E

C

C

K

R

Q

A

Y

L

G

A

E

Q

S

N

G

G

A

G

R

N

I

I

T

T

L

L

T

T

E

E

D

D

P

V

K

A

A

K

A

G

V

V

K

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

V

K

E

E

A

K

W

W

A

A

E

E

R

R

I

I

Q

A

Q

L

L

L

L

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

K

Q

A

L

T

T

G

G

N

N

P

P

R

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

D

C

D

D

Q

T

T

K

T

V

L

G

G

K

K

Q

K

I

M

A

A

E

E

Q

E

Y

F

P

G

Y

-

L

L

S

H

N

G

G

G

I

M

E

E

V

V

T

T

D

D

D

E

V

V

F

F

E

E

S

S

P

A

A

A

C

S

I

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A
|
A

I

V

L

M

V

V

S

A

T

T

L

L

A

S

S56 (= S57) mutation to H: Much less active than the wild-type.
STRT 56:59 (= STRT 57:60) binding carbamoyl phosphate
R58 (= R59) mutation to G: The mutant is drastically inefficient in catalysis, but affects only moderately the binding of carbamoyl phosphate.
Q83 (= Q84) binding carbamoyl phosphate
K87 (= K88) mutation to Q: Much less active than the wild-type.
R107 (= R108) binding carbamoyl phosphate
HPTQ 134:137 (= HPTQ 135:138) binding carbamoyl phosphate
N168 (= N168) binding L-ornithine
D232 (= D232) binding L-ornithine
SM 236:237 (= SM 236:237) binding L-ornithine
C274 (= C274) binding Zn(2+); mutation to A: Zinc ion is no longer a tight-binding inhibitor and does not promote isomerization.
CL 274:275 (= CL 274:275) binding carbamoyl phosphate
R320 (= R321) binding carbamoyl phosphate; mutation to A: Much less active than the wild-type.
A326 (= A327) mutation to G: Activity greater than the wild-type and Km for ornithwinas increases about twofold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1duvG Crystal structure of e. Coli ornithine transcarbamoylase complexed with ndelta-l-ornithine-diaminophosphinyl-n-sulphonic acid (psorn) (see paper)
60% identity, 99% coverage: 4:334/336 of query aligns to 2:332/333 of 1duvG

query
sites
1duvG

N

G

I

F

H

Y

N

H

R

K

N

H

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

T

P

P

R

A

E

E

L

L

R

N

Y

S

L

L

D

Q

L

L

S

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

T

S

G

G

T

K

E

E

Q

E

Q

A

H

K

L

L

K

T

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

Y

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

I

L

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H

H

K

K

E

E

S

S

M

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

Y

K

G

Q

Q

E

E

I

I

V

V

E

E

E

T

L

L

A

A

K

E

F

Y

A

A

G

S

V

V

P

P

V

V

F

W

N

N

G

G

L
|
L

T

T

D

N

E

E

Y

F

H
|
H

P

P

T

T

Q

Q

M

L

I

L

A

A

D

D

V

L

L

L

T

T

M

M

R

Q

E

E

H

H

-

L

A

P

D

G

K

K

P

A

I

F

H

N

E

E

I

M

S

T

Y

L

A

V

Y

Y

L

A

G

G

D

D

A

A

R

R

N

N

N
|
N

M

M

G

G

N

N

S

S

L

M

L

L

L

E

I

A

G

A

A

A

K

L

L

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

A

A

P

P

K

Q

A

A

L

C

W

W

P

P

A

E

D

A

L

L

V

V

A

T

R

E

C

C

K

R

Q

A

Y

L

G

A

E

Q

S

N

G

G

A

G

R

N

I

I

T

T

L

L

T

T

E

E

D

D

P

V

K

A

A

K

A

G

V

V

K

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

V

K

E

E

A

K

W

W

A

A

E

E

R

R

I

I

Q

A

Q

L

L

L

L

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

K

Q

A

L

T

T

G

G

N

N

P

P

R

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

D

C

D

D

Q

T

T

K

T

V

L

G

G

K

K

Q

K

I

M

A

A

E

E

Q

E

Y

F

P

G

Y

-

L

L

S

H

N

G

G

G

I

M

E

E

V

V

T

T

D

D

D

E

V

V

F

F

E

E

S

S

P

A

A

A

C

S

I

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

S

binding ndelta-(n'-sulphodiaminophosphinyl)-l-ornithine: S55 (= S57), T56 (= T58), R57 (= R59), T58 (= T60), R106 (= R108), L128 (= L130), H133 (= H135), N167 (= N168), D231 (= D232), S235 (= S236), M236 (= M237), C273 (= C274), L274 (= L275), R319 (= R321)

2otcA Ornithine transcarbamoylase complexed with n-(phosphonacetyl)-l- ornithine (see paper)
60% identity, 99% coverage: 4:334/336 of query aligns to 2:332/333 of 2otcA

query
sites
2otcA

N

G

I

F

H

Y

N

H

R

K

N

H

L

F

L

L

S

K

L

L

E

L

H

D

H

F

T

T

P

P

R

A

E

E

L

L

R

N

Y

S

L

L

D

Q

L

L

S

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

T

S

G

G

T

K

E

E

Q

E

Q

A

H

K

L

L

K

T

G

G

N

K

N

N

I

I

A

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

Y

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

I

L

D

G

P

P

N

S

S

G

S

S

Q

Q

I

I

G

G

H
|
H

K

K

E

E

S

S

M

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

F

Y

K

G

Q

Q

E

E

I

I

V

V

E

E

E

T

L

L

A

A

K

E

F

Y

A

A

G

R

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

N

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

L

I

L

A

A

D

D

V

L

L

L

T

T

M

M

R

Q

E

E

H

H

-

L

A

P

D

G

K

K

P

A

I

F

H

N

E

E

I

M

S

T

Y

L

A

V

Y

Y

L

A

G

G

D

D

A

A

R

R

N

N

N
|
N

M

M

G

G

N

N

S

S

L

M

L

L

L

E

I

A

G

A

A

A

K

L

L

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

A

A

P

P

K

Q

A

A

L

C

W

W

P

P

A

E

D

A

L

L

V

V

A

T

R

E

C

C

K

R

Q

A

Y

L

G

A

E

Q

S

N

G

G

A

G

R

N

I

I

T

T

L

L

T

T

E

E

D

D

P

V

K

A

A

K

A

G

V

V

K

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

V

K

E

E

A

K

W

W

A

A

E

E

R

R

I

I

Q

A

Q

L

L

L

L

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

K

Q

A

L

T

T

G

G

N

N

P

P

R

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

F

F

H

H

N

D

C

D

D

Q

T

T

K

T

V

L

G

G

K

K

Q

K

I

M

A

A

E

E

Q

E

Y

F

P

G

Y

-

L

L

S

H

N

G

G

G

I

M

E

E

V

V

T

T

D

D

D

E

V

V

F

F

E

E

S

S

P

A

A

A

C

S

I

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

I

V

L

M

V

V

S

A

T

T

L

L

A

S

active site: R57 (= R59), T58 (= T60), H85 (= H87), R106 (= R108), H133 (= H135), Q136 (= Q138), D231 (= D232), C273 (= C274), R319 (= R321)
binding n-(phosphonoacetyl)-l-ornithine: S55 (= S57), T56 (= T58), R57 (= R59), T58 (= T60), R106 (= R108), H133 (= H135), N167 (= N168), D231 (= D232), S235 (= S236), M236 (= M237), L274 (= L275), R319 (= R321)

Q8G998 Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 from Lentilactobacillus hilgardii (Lactobacillus hilgardii) (see paper)
50% identity, 99% coverage: 5:336/336 of query aligns to 10:338/343 of Q8G998

query
sites
Q8G998

I

F

H

Q

N

G

R

R

N

S

L

V

L

L

S

A

L

E

E

K

H

D

H

F

T

S

P

A

R

A

E

E

L

L

R

E

Y

Y

L

L

L

I

D

D

L

F

S

G

R

L

D

H

L

L

K

K

R

A

A

L

K

K

Y

K

T

A

G

G

T

I

E

P

Q

H

H

Y

L

L

K

E

G

G

N

K

N

N

I

I

A

A

L

L

I

L

F

F

E

E

K

K

T

S

S

S

T

T

R

R

T

T

R

R

C

S

A

A

F

F

E

T

V

T

A

A

A

S

Y

I

D

D

Q

L

G

G

A

A

N
x
H

V

P

T

E

Y

Y

I

L

D

G

P

Q

N

N

S

D

S

I

Q

Q

I

L

G

G

H

K

K

K

E

E

S

S

M

T

K

S

D

D

T

T

A

A

R

K

V

V

L

L

G

G

R

S

M

M

Y

F

D

D

A

G

I

I

E

E

Y

F

R

R

G

G

F

F

K

K

Q

Q

E

S

I

D

V

A

E

E

E

I

L

L

A

A

K

R

F

D

A

S

G

G

V

V

P

P

V

V

F

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

W

H

H

P

P

T

T

Q

Q

M

M

I

L

A

A

D

D

V

F

L

M

T

T

M

V

R

K

E

E

H

N

A

F

D

G

K

K

P

-

I

L

H

Q

E

G

I

L

S

T

Y

L

A

T

Y

F

L

M

G

G

D

D

A

G

R

R

N

N

M

V

G

A

N

N

S

S

L

L

L

V

I

T

G

G

A

A

K

I

L

L

G

G

M

V

D

N

V

I

R

H

I

I

C

V

A

A

P

P

K

K

A

A

L

L

W

F

P

P

A

T

D

E

L

T

V

Q

A

N

R

I

C

A

K

K

Q

G

Y

F

G

A

E

E

E

K

S

S

G

G

A

A

R

K

I

L

T

V

L

I

T

T

E

D

D

D

P

L

K

D

A

E

A

G

V

L

K

K

G

G

V

S

D

N

F

V

I

V

H

Y

T

T

D

D

V

V

W

W

V

V

S

S

M

M

G

G

E

E

P

S

V

-

E

-

A

N

W

W

A

E

E

E

R

R

I

V

Q

K

Q

E

L

L

L

T

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

E

L

A

M

M

K

K

A

K

T

T

G

G

N

T

P

P

R

D

T

D

K

Q

-

L

-

I

F

F

M

M

H

H

C

C

L

L

P

P

A

A

F

F

H

H

N

N

C

T

D

D

T

T

K

Q

V

Y

G

G

K

K

Q

E

I

I

A

K

E

E

Q

K

Y

Y

P

G

Y

I

L

T

S

E

N

-

G

-

I

M

E

E

V

V

T

T

D

D

D

E

V

V

F

F

E

T

S

S

P

K

A

Y

C

A

I

R

A

Q

F

F

E

E

Q

E

A

A

E

E

N

N

R

R

M

M

H

H

T

S

I

I

K

K

A

A

I

M

L

M

V

A

S

A

T

T

L

L

A

G

D
x
N

L
|
L

H79 (≠ N74) binding Ni(2+)

Sites not aligning to the query:

337:343 mutation Missing: It generates a metastable mutant that behaves as a mixture of monomeric and trimeric species with only the latter exhibiting OTC activity.

Q51742 Ornithine carbamoyltransferase, anabolic; OTCase; EC 2.1.3.3 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 3 papers)
39% identity, 100% coverage: 1:336/336 of query aligns to 1:313/315 of Q51742

query
sites
Q51742

M
|
M

A

V

F

V

N

S

I

L

H

A

N

G

R

R

N

D

L

L

S

C

L

L

E

Q

H

D

H

Y

T

T

P

A

R

E

E

E

L

I

R
x
W

Y

T

L

I

L

L

D
x
E

L

T

S

A

R

K

D
x
M

L

F

K

K

R

I

A
x
W

K

Q

Y

K

T

I

G

G

T

K

E

P

Q

H

Q

R

H

L

L

L

K

E

G

G

N

K

N

T

I

L

A

A

L

M

I

I

F

F

E

Q

K

K

T

P

S

S

T

T

R

R

T

T

R

R

C

V

A

S

F

F

E

E

V

V

A

A

A

M

Y

A

D

H

Q

L

G

G

A

G

N

H

V

A

T

L

Y

Y

I

L

D

N

P

A

N

Q

S

D

S

L

Q

Q

I

L

G

R

H

R

K

G

E

E

S

T

M

I

K

A

D

D

T

T

A

A

R

R

V

V

L

L

G

S

R

R

M

Y

Y

V

D

D

A

A

I

I

E

M

Y

A

R

R

G

V

F

Y

K

D

Q

H

E

K

I

D

V

V

E

E

E

D

L

L

A

A

K

K

F

Y

A

A

G

T

V

V

P

P

V

V

F

I

N

N

G

G

L

L

T

S

D

D

E

F

Y

S

H

H

P

P

T

C

Q

Q

M

A

I

L

A

A

D

D

V

Y

L

M

T

T

M

I

R

W

E

E

H

K

A

K

D

G

K

T

P

-

I

I

H

K

E

G

I

V

S

K

Y

V

A

V

Y

Y

L

V

G

G

D

D

A

G

R

-

N

N

M

V

G

A

N

H

S

S

L

L

L

M

L

I

I

A

G

G

A

T

K

K

L

L

G

G

M

A

D

D

V

V

R

V

I

V

C

A

A

T

P

P

K

E

A

G

L

Y

W

E

P

P

A

D

D

E

D

K

L

V

V

I

A

K

R

W

C

A

K

E

Q

Q

Y

N

G

A

E

A

E

E

S

S

G

G

A

G

R

S

I

F

T

E

L

L

T

L

E

H

D

D

P

P

K

V

A

K

A

A

V

V

K

K

G

D

V

A

D

D

F

V

I

I

H
x
Y

T

T

D

D

V

V

W

W

V

A

S

S

M

M

G

G

E

Q

P

E

V

A

E

E

A
|
A

W

-

A

E

E

E

R

R

I

R

Q

K

Q

I

L

F

L

R

P

P

Y

F

Q

Q

V

V

N

N

A

K

E

D

L

L

M

V

K

K

A

-

T

H

G

A

N

K

P

P

R

D

T

Y

K

M

F

F

M

M

H

H

C

C

L

L

P

P

A

A

F

-

H

-

N

-

C

-

D

-

T

-

K

-

V

-

G

-

K

-

Q

-

I

-

A

-

E

-

Q

-

Y

-

P

-

Y

-

L

-

S

H

N

R

G

G

I

E

E
|
E

V

V

T

T

D

D

V

V

F

I

E

D

S

S

P

P

A

N

C

S

I

V

A

V

F

W

E

D

Q

Q

A

A

E

E

N

N

R

R

M

L

H

H

T

A

I

Q

K

K

A

A

I

V

L

L

V

A

S

L

T

V

L

M

A

G

D

G

L

I

M1 (= M1) modified: Initiator methionine, Removed
W22 (≠ R22) mutation to A: Decreased heat stability.
E26 (≠ D26) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ D30) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ A34) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (≠ H230) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (= A243) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E301) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

P00481 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Rattus norvegicus (Rat) (see 2 papers)
38% identity, 99% coverage: 4:335/336 of query aligns to 36:344/354 of P00481

query
sites
P00481

N

Q

I

L

H

K

N

G

R

R

N

D

L

L

S

T

L

L

E

K

H

N

H

F

T

T

P

G

R

E

E

E

L

I

R

Q

Y

Y

L

M

L

L

D

W

L

L

S

S

R

A

D

D

L

L

K

K

-

F

R

R

A

I

K

K

Y

Q

T

K

G

G

T

E

E

Y

Q

L

Q

P

H

L

L

L

K

Q

G

G

N

K

N

S

I

L

A

G

L

M

I

I

F

F

E

E

K

K

T

R

S

S

T

T

R
|
R

T

T

R

R

C

L

A

S

F

T

E

E

V

T

A

G

A

F

Y

A

D

L

Q

L

G

G

A

G

N

H

V

P

T

S

Y

F

I

L

D

T

P

T

N

Q

S

D

S

I

Q

H

I

L

G

G

H

V

K

N

E

E

S

S

M

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

G

S

R

S

M

M

Y

T

D

D

A

A

I

V

E

L

Y

A

R

R

G

V

F

Y

K

K

Q

Q

E

S

I

D

V

L

E

D

E

I

L

L

A

A

K

K

F

E

A

A

G

T

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I

P

P

V

I

F

V

N

N

G

G

L

L

T

S

D

D

E

L

Y

Y

H

H

P

P

T

I

Q

Q

M

I

I

L

A

A

D

D

V

Y

L

L

T

T

M

L

R

Q

E

E

H

H

A

Y

D

G

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S

P

-

I

L

H

K

E

G

I

L

S

T

Y

L

A

S

Y

W

L

I

G

G

D

D

A

G

R

-

N

N

M

I

G

L

N

H

S

S

L

I

L

M

I

S

G

A

A

A

K

K

L

F

G

G

M

M

D

H

V

L

R

Q

I

A

C

A

A

T

P

P

K

K

A

G

L

Y

W

E

P

P

A

D

D

P

D

N

L

I

V

V

A

K

R

L

C

A

K

E

Q

Q

Y

Y

G

A

E

K

E

E

S

N

G

G

A

T

R

R

I

L

T

S

L

M

T

T

E

N

D

D

P

P

K

L

A

E

A

A

V

A

K

R

G

G

V

G

D

N

F

V

I

L

H

I

T

T

D

D

V

T

W

W

V

I

S

S

M

M

G

G

E

Q

P

E

V

D

E

E

A

K

W

-

A

K

E

K

R

R

I

L

Q

Q

Q

A

L

F

L

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

K

K

A

V

T

A

G

A

N

S

P

D

R

W

T

T

K

-

F

F

M

L

H

H

C
|
C

L

L

P

P

A

-

F

-

H

-

N

-

C

-

D

-

T

-

K

-

V

-

G

-

K

-

Q

-

I

-

A

-

E

-

Q

R

Y

K

P

P

Y

E

L

-

S

-

N

-

G

-

I

-

E

E

V

V

T

D

D

D

D

E

V

V

F

F

E

Y

S

S

P

P

A

R

C

S

I

L

A

V

F

F

E

P

Q

E

A

A

E

E

N

N

R

R

M

K

H

W

T

T

I

I

K

M

A

A

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L

M

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L

L

A

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D

D

R92 (= R59) mutation to L: Strong decrease in ornithine carbamoyltransferase activity.
C303 (= C274) mutation to S: Increases KM for ornithine 5-fold and decreases kcat 20-fold.

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
37% identity, 99% coverage: 5:336/336 of query aligns to 9:312/316 of Q81M99

query
sites
Q81M99

I

L

H

N

N

T

R

K

N

D

L

L

S

T

L

L

E

E

H

E

H

L

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T

P

Q

R

E

E

E

L

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R

I

Y

S

L

L

L

I

D

E

L

F

S

A

R

I

D

Y

L

L

K

K

R

K

A

N

K

K

Y

-

T

-

G

-

T

-

E

Q

Q

E

Q

P

H

L

L

L

K

Q

G

G

N

K

N

I

I

L

A

G

L

L

I

I

F

F

E

D

K

K

T

H

S
|
S

T
|
T

R
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R

T
|
T

R

R

C

V

A

S

F

F

E

E

V

A

A

G

A

M

Y

V

D

Q

Q

L

G

G

A

G

N

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V

G

T

M

Y

F

I

L

D

N

P

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Q

I

M

G

G

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K

G

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M

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S

D

D

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T

A

A

R

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D

A

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R

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E

L

L

A

A

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P

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I

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N

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L

L

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E

D

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H

H
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H

P
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P

T
x
C

Q
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Q

M

A

I

L

A

A

D

D

V

L

L

M

T

T

M

I

R

Y

E

E

H

E

A

T

D

N

K

-

P

T

I

F

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I

S

K

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L

A

A

Y

Y

L

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G

G

D

D

A

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R

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N

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N
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N

M

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C

N

H

S

S

L

L

I

A

G

S

A

A

K

K

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M

M

D

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M

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C

A

A

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K

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P

A

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A

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A

Y

I

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A

E

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E

E

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G

G

A

A

R

E

I

I

T

E

L

I

T

L

E

H

D

N

P

P

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E

A

L

A

A

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K

N

G

E

V

A

D

D

F

F

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I

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T

T

D
|
D

V

V

W

W

V

M

S
|
S

M
|
M

G

G

E

Q

P

-

V

-

E

E

A

G

W

E

A

E

E

E

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K

I

Y

Q

T

Q

L

L

F

L

Q

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P

Y

Y

Q

Q

V

I

N

N

A

K

E

E

L

L

M

V

K

K

A

H

T

-

G

A

N

K

P

Q

R

T

T

Y

K

H

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

F

-

H

H

N

R

C

E

D

E

T

-

K

-

V

-

G

-

K

-

Q

-

I

-

A

-

E

-

Q

-

Y

-

P

-

Y

-

L

-

S

-

N

-

G

-

I

-

E

E

V

V

T

T

D

G

D

E

V

I

F

I

E

D

S

G

P

P

A

Q

C

S

I

I

A

V

F

F

E

E

Q

Q

A

A

E

G

N

N

R
|
R

M

L

H

H

T

A

I

Q

K

K

A

A

I

L

L

L

V

V

S

S

T

L

L

F

A

K

D

N

L

V

STRT 57:60 (= STRT 57:60) binding carbamoyl phosphate
Q84 (= Q84) binding carbamoyl phosphate
R108 (= R108) binding carbamoyl phosphate
HPCQ 135:138 (≠ HPTQ 135:138) binding carbamoyl phosphate
N166 (= N168) binding L-ornithine
D230 (= D232) binding L-ornithine
SM 234:235 (= SM 236:237) binding L-ornithine
CL 269:270 (= CL 274:275) binding carbamoyl phosphate
R297 (= R321) binding carbamoyl phosphate

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
37% identity, 99% coverage: 5:335/336 of query aligns to 37:344/354 of P00480

query
sites
P00480

I

L

H

K

N
x
G

R
|
R

N

D

L

L

L
|
L

S

T

L

L

E
x
K

H

N

H

F

T

T

P

G

R

E

E

E

L

I

R

K

Y
|
Y

L

M

L

L

D

W

L

L

S

S

R

A

D

D

L
|
L

K

K

-

F

R

R

A

I

K

K

Y

Q

T

K

G

G

T

E

E

Y

Q

L

Q

P

H

L

L

L

K

Q

G

G

N

K

N

S

I

L

A

G

L

M

I

I

F

F

E

E

K
|
K

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|
S

T
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T

R
|
R

T
|
T

R

R

C

L

A

S

F

T

E

E

V

T