SitesBLAST

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 89% coverage: 18:405/436 of query aligns to 69:472/582 of Q9JI12

query
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Q9JI12

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R88 (= R37) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
H128 (≠ F62) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R116) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E123) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ L257) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
25% identity, 89% coverage: 18:405/436 of query aligns to 11:414/452 of 7t3nA

query
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7t3nA

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binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y69), Y137 (= Y127), Y165 (≠ F155), R264 (≠ L257), F268 (≠ L261), Y269 (≠ W262)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: R12 (= R19), Y13 (≠ F20), E152 (= E142), G163 (= G153)

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
24% identity, 83% coverage: 59:420/436 of query aligns to 109:467/495 of Q8BN82

query
sites
Q8BN82

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H183 (≠ S131) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 86% coverage: 59:433/436 of query aligns to 109:484/495 of Q5Q0U0

query
sites
Q5Q0U0

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L

L

R
|
R

M

A

A

L

V
x
E

G
|
G

A

L

L

G

E
|
E

A
x
G

P

V

A

T

Y
x
F

P
|
P

I

A

N

M

S
x
H

R

A

V

M

V
x
W

T

S

W

W

F

A

P

P

E

P

R

L

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E

R

R

A

S

T

K

A

L

I

L

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F

I

Y

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Y

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G

G

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A

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Q

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G

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W

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V

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G

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V

G

G

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I

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W

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K

G

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N

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A

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E

I

K

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L

Y

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-

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-

G

-

L

-

V

V

D
x
S

I
x
S

Q
x
L

A
x
K

E
x
N

Q

Q

A

L

R

S

V

S

K

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A

K

K

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F

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W

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W

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N

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Y

Y

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K

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P

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|
G

L

M

-

V

-

G

-

P

-

A

-

V

-

F

L

L

I

V

S

A

T

A

S

G

I

F

I

I

G

G

A

C

N

D

F

Y

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-

E

-

S

-

T

-

P

S

L

L

V

A

I

V

A

A

F

F

L

L

A

T

L

I

A

S

F

T

G

L

N

G

G

G

L

F

A

A

S

S

I

S

T

G

W

F

S

S

L

I

V

N

S

H

T

L

L

D

A

I

P

A

A

P

R

S

L

Y

L

A

G

G

L

I

T

L

G

L

G

G

V

I

F

T

N

N

F

T

I

F

G

A

N

T

L

I

S

P

A

G

I

M

A

I

T

G

P

P

I

I

V

I

I

A

G

K

F

S

L

L

A

T

T

P

G

D

D

N

S

T

F

I

A

K

P

E

A

W

I

Q

T

T

Y

V

I

F

S

C

V

I

L

A

A

A

L

A

I

I

-

N

-

V

-

F

G

G

A

A

L

I

S

F

Y

F

I

T

L

L

L

F

V

A

-

K

G

G

K

E

V

V

E

Q

R

N

K136 (≠ R86) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R116) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ V119) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G120) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E123) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A124) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ Y127) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P128) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ S131) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ V134) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ DIQAE 221:225) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P290) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (= G326) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 67% coverage: 19:312/436 of query aligns to 15:338/493 of Q03567

query
sites
Q03567

R

R

F

F

F

A

I

L

M

S

V

L

L

V

L

M

F

F

I

F

T

G

V

C

V

L

I

V

N

T

Y

Y

L

M

D

M

R

R

S

T

N

N

L

M

S

S

I

F

A

A

A

V

P

V

A

C

L

M

-

V

-

N

-

E

-

N

T

K

S

T

D

D

L

T

G

G

I

V

D

E

P

K

I

V

H

S

-

R

-

C

-

G

-

K

-

E

-

M

-

T

-

P

-

V

-

E

-

S

-
x
N

-

S

-

V

-

I

-

G

-

E

-

F

-

D

-

W

-

D

-

K

-

Q

-

T

V

T

G

G

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M

I

V

F

L

S

S

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

A

G

M

S

Q

Q

I

I

P

I

G

G

W

H

L

L

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V

Y

P

G

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I

R

L

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Y

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F

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V

A

T

L

I

L

L

L

G

W

S

S

A

V

L

A

L

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T

V

L

M

L

L

N

G

P

F

V

A

A

G

A

-

R

-

T

S

S

F

E

I

Y

A

A

L

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F

A

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I

L

L

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R

M

A

A

A

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I

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G

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Q

A

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A

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G

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V

F

G

G

I

V

V

L

W

W

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T

A

A

V

V

I

W

W

W

Y

Y

A

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V

S

Y

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K

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A

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H

K

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R

A

I

N

T

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P

A

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E

E

I

K

D

Q

L

Y

I

I

R

-

E

-

G

-

L

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V

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D

A

I

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Q

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A

A

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Q

M

A

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A

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I

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K

S

R

P

K

A

L

V

W

W

G

A

I

C

Y

W

L

A

G

G

Q

H

F

F

C

A

L

G

N

D

S

W

T

G

L

A

W

Y

F

T

F

M

L

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W

S

F

L

P

P

T

S

Y

F

L

L

V

K

K

D

Y

V

R

L

G

G

M

L

D

N

F

L

I

S

K

S

S

L

G

G

L

A

L

V

A

A

S

S

L

I

P

P

F

Y

L

I

A

A

A

Y

F

F

I

L

G

A

V

I

L

N

C

A

S

G

G

G

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V

F

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A

D

D

F

T

L

L

I

R

R

S

R

K

G

G

N69 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 86% coverage: 59:433/436 of query aligns to 109:484/495 of Q9NRA2

query
sites
Q9NRA2

G

G

L

W

I

I

F

L

S

G

A

S

F

F

G

F

W

Y

T

G

Y

Y

A

I

M

T

Q

Q

I

I

P

P

G

G

W

Y

L

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V

A

D

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V

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G

R
x
K

I

M

L

L

Y

L

S

G

V

F

A

G

L

I

L

L

L

G

W

T

S

A

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V

A

L

T

T

V

L

M

F

L

T

G

P

F

I

A

A

G

A

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D

F

L

I

G

A

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-

G

-

P

L

L

F

I

V

V

L

L

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R

M

A

A

L

V

E

G

G

A

L

L

G

E

E

A

G

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A

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Y

F

P

P

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A

N

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x
H

R

A

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M

V

W

T

S

W

W

F

A

P

P

E

P

R

L

E

E

R

R

A

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T

K

A
x
L

I
x
L

G

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F

I

Y

S

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Y

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A

G

G

Q

A

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Q

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G

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N

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W

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V

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F

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Y

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G

A

T

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I

G

G

I

I

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W

W

A

F

V

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I

L

W

W

-

I

Y

W

A

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V

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Q

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H

K

K

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R

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A

Y

E

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I

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D

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I
|
I

R

-

E

-

G

-

L

-

V
x
L

D
x
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I
x
S

Q
x
L

A
x
R

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x
N

Q

Q

A

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Q

A

K

K

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V

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W

T

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-

I

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V

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K

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L

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W

G

A

I

I

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A

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H

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F

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Y

N

N

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L

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W

Y

F

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F

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L

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W

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T

T

Y

Y

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K

E

Y

I

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L

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R

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N

F

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E

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G
|
G

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L

A

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S

S

L

L

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|
P

F

Y

L

L

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A

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M

L

I

C

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S

G

G

F

Q

F

A

S

A

D

D

F

N

L

L

I

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R

A

R

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-

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T

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R

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R

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F

I

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I

L

S

I

G
|
G

L

M

-

I

-

G

-

P

-

A

-

V

-

F

L

L

I

V

S

A

T

A

S

G

I

F

I

I

G

G

A

C

N

D

F

Y

V

-

E

-

S

-

T

-

P

S

L

L

V

A

I

V

A

A

F

F

L

L

A

T

L

I

A

S

F

T

G

L

N

G

G

G

L

F

A

C

S

S

I

S

T

G

W

F

S

S

L

I

V

N

S

H

T

L

L

D

A

I

P

A

A

P

R

S

L

Y

L

A

G

G

L

I

T

L

G

L

G

G

V

I

F

T

N

N

F

T

I

F

G

A

N

T

L

I

S

P

A

G

I

M

A

V

T

G

P

P

I

V

V

I

I

A

G

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F

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L

L

A

T

T

P

G

D

D

N

S

T

F

V

A

G

P

E

A

W

I

Q

T

T

Y

V

I

F

S

Y

V

I

L

A

A

A

L

A

I

I

-

N

-

V

-

F

G

G

A

A

L

I

S

F

Y

F

I

T

L

L

L

F

V

A

-

K

G

G

K

E

V

V

E

Q

R

N

K136 (≠ R86) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ S131) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 146:147) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
I------L 266:267 (≠ IREGGGLV 213:220) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ DIQAE 221:225) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G284) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P290) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (= G326) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
23% identity, 81% coverage: 54:405/436 of query aligns to 106:455/497 of Q9Y2C5

query
sites
Q9Y2C5

D

S

P

P

I

E

H

I

V

Q

G

G

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I

I

I

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A

S

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N

W

Y

T

G

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A

F

A

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A

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P

I

I

P

P

G

S

G

G

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Y

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V

A

D

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G

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K

I

Y

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Y

V

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G

V

A

A

G

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L

L

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L

I

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S

S

S

V

F

A

L

T

T

V

L

M

F

L

I

G

P

F

L

A

A

G

A

S

N

-

A

-

G

F

V

I

A

A

L

L

L

F

I

V

V

L

L

R

R

M

I

A

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V

Q

G

G

A

I

L

A

E

Q

A

V

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M

A

V

Y

L

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T

I

G

N

Q

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Y

R

S

V

I

V

W

T

V

T

K

W

W

F

A

P

P

E

P

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E

E

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R

A

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A

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T

G

T

F

I

Y

A

T

G

S

S

G

G

Q

S

F

M

V

L

G

G

L

S

A

F

F

I

L

V

T

L

P

L

V

A

L

G

A

G

W

L

L

L

Q

C

H

Q

E

T

F

I

G

G

W

W

H

P

M

Y

V

V

F

F

V

Y

T

I

T

F

G

G

A

G

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I

G

G

I

C

V

A

W

C

A

C

V

P

I

L

W

W

Y

F

A

P

-

L

V

I

Y

Y

R

D

E

D

P

P

R

V

D

N

F

H

K

P

G

F

A

I

N

S

D

A

A

G

E

E

I

K

D

R

L

Y

I

I

R

V

E

-

G

-

L

-

V

-

D

-

I

-

Q

-

A

C

E

S

Q

L

A

A

R

Q

V

Q

K

D

A

C

K

S

F

P

S

G

W

W

T

S

-

L

D

P

L

I

G

R

I

A

V

M

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K

K

S

R

L

K

P

L

L

W

W

G

A

I

I

Y

L

L

V

G

S

Q

Y

F

F

C

C

L

E

N

Y

S

W

T

L

L

F

W

Y

F

T

F

I

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M

T

A

W

Y

F

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P

P

T

T

Y

Y

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I

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S

Y

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L

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Q

M

A

D

N

F

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G

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I

L

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A

S

S

A

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P

P

F

F

L

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A

V

A

G

F

C

I

I

G

C

V

I

L

I

C

L

S

G

G

G

F

L

S

A

D

D

F

F

L

L

I

L

-

S

R

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R

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G

I

Y

L

T

R

V

L

G

I

F

T

A

I

R

R

K

K

L

L

P

F

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I
x
A

S

I

G

G

L

V

L

L

I

F

S

P

T

S

S

V

I

I

-

L

I

V

G

S

A

L

N

P

F

W

V

V

E

R

S

S

T

S

-

H

P

S

L

M

V

T

I

M

A

T

F

F

L

L

A

V

L

L

A

S

F

-

G

-

N

S

G

A

L

I

A

S

S

S

I

F

T

C

W

E

S

S

-

G

-

A

L

L

V

V

S

N

T

F

L

L

A

D

P

I

A

A

-

P

R

R

L

Y

L

T

G

G

L

F

T

L

G

K

G

G

V

L

F

L

N

Q

F

V

I

F

G

A

N

H

L

I

S

A

A

G

I

A

A

I

T

S

P

P

I

T

V

A

I

A

G

G

F

F

L

F

A

I

T

S

G

Q

D

D

S

S

A372 (≠ I324) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
24% identity, 60% coverage: 16:275/436 of query aligns to 24:280/448 of Q51955

query
sites
Q51955

S

S

R

R

K

Y

R

Q

F

W

F

R

I

V

M

V

V

L

L

L

L

C

F

F

I

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T

I

V

V

V

F

I

L

N
x
D

Y

G

L

L

D
|
D

R

T

S

A

N

A

L

M

S

G

I

F

A

I

A

A

P

P

A

A

L

L

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S

S

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E

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W

G

G

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I

D

D

P

R

I

A

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S

V

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G

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P

I

V

F

M

S

S

A

A

-

A

-

L

F

I

G

G

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M

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V

Y

F

A

G

A

A

M

L

Q

G

I

-

P

-

G

S

G
|
G

W

P

L

L

V

A

D
|
D

R

R

V

F

P
x
G

P

R

R

K

I

G

L

V

Y

L

S

V

V

G

A

A

L

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L

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L

V

W

F

S

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V

G

A

F

T

S

V

L

M

A

L

S

G

A

F

Y

A

A

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T

S

N

F

V

I

D

A

Q

L

L

F

L

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L

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R
|
R

M

F

A

L

V

T

G

G

A

L

L

G

E

L

A

G

P

A

A

G

Y

M

P

P

I

N

N

A

S

T

R

T

V

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S

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x
E

W

Y

F

T

P

P

E

E

R

R

E

L

R

K

A

S

T

L

A

L

I

V

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T

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S

Y

M

T

F

S

C

G

G

Q

F

F

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V

L

G

G

L

M

A

A

F

G

L

G

T

G

P

F

V

I

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S

A

A

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K

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M

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I

H

P

E

A

F

Y

G

G

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W

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H

M

S

V

L

F

L

V

V

T

I

T

G

G

G

A

V

V

L

G

P

I

L

V

L

W

L

A

A

V

L

I

V

W

L

Y

M

A

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V

W

Y

L

R

P

E

E

P

S

R

A

D

R

F

F

K

L

G

V

A

V

N

R

D

N

A

R

E

G

I

T

D

D

L

K

I

I

R

R

E

K

G

-

G

T

L

L

V

S

D

P

I

I

Q

A

A

P

E

Q

Q

V

A

V

R

A

V

E

K

A

A

G

K

S

F

F

S

S

W

V

T

P

D

E

L

Q

G

K

I

A

V

V

L

A

T

A

K

R

R

S

K

V

L

F

W

A

G

V

I

I

Y

F

L

S

G

G

Q

T

F

Y

C

G

L

L

N

G

S

-

T

T

L

M

W

L

F

L

F

W

L

L

T

T

W

Y

F

F

P

M

T

G

Y

L

L

V

V

I

K

V

Y

Y

D41 (≠ N33) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D36) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (= G77) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (= D81) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (≠ P84) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R116) mutation to A: Abolishes 4-HBA transport.
E144 (≠ T136) mutation to A: Strong decrease in 4-HBA transport.
H183 (= H175) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
22% identity, 82% coverage: 11:369/436 of query aligns to 99:449/605 of Q9GQQ0

query
sites
Q9GQQ0

S

S

L

A

V

V

T

V

P

P

S

S

R

R

-

L

-

S

-

V

-

G

-

R

-

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Q

R

W

F

F

F

T

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M

T

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V

L

L

L

C

F

F

I

V

T

N

V

L

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-

I

I

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N

Y

Y

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M

D

D

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R

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F

N

T

L

I

S

A

I

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A

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A

D

L

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T

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D

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G

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A

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F

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A

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R

M

A

A

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V

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G

A

I

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E
|
E

A

A

P

S

A

Y

Y

S

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T

I

I

N

A

S

P

R

T

V

I

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S

T

D

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E

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E

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A

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A

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A

Q

I

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V

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G

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S

A

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F

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Y

P

I

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V

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A

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Q

A

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H

-

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E

A

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N

G

D

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W

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W

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A

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A

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I

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Y

I

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K

E

D

P

P

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-

D

-

F

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K

R

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G

A

H

N

S

D

E

A

G

E

S

I

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D

N

L

L

I

-

R

-

E

-

G

-

L

-

V

-

D

-

I

-

Q

-

A

-

E

-

Q

-

A

-

R

-

V

-

K

E

A

A

K

T

F

T

S

Y

W

K

T

Q

D

D

L

I

G

K

I

A

V

L

L

V

T

R

K

N

R

R

K

S

L

F

W

M

G

L

I

S

Y

T

L

A

G

G

Q

F

F

T

C

C

L

V

N

A

S

F

T

V

L

A

W

G

F

A

F

L

L

A

T

W

W

W

F

G

P

P

T

S

Y

F

L

I

V

-

K

-

Y

Y

R

L

G

G

M

M

-

K

-

M

-

Q

-

P

-

G

-

N

-

E

D

N

F

I

I

V

K

Q

S

D

G

D

L

V

-

A

-

F

-

N

L

F

A

G

S

V

L

I

P

T

F

M

L

L

A

A

A

G

F

L

I

L

G

G

V

V

L

P

C

L

S

G

G

S

F

F

F

L

S

S

D

Q

F

Y

L

L

I

V

R

K

R

R

G

-

Y

-

T

-

V

-

G

-

F

Y

A

P

R

T

K

A

L

D

P

P

I

V

I

I

S

C

-

A

-

F

G

G

L

L

I

V

S

S

T

A

S

P

I

L

G

T

A

G

N

A

F

C

V

L

E

L

S

V

T

N

P

S

L

N

V

S

I

V

A

G

F

T

L

Y

A

A

L

L

A

I

F

F

G

G

N

Q

G

L

L

A

A

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S

N

I

L

T

N

W

W

S

A

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V

S

A

T

D

L

I

E217 (= E123) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 60% coverage: 18:280/436 of query aligns to 19:288/452 of Q5EXK5

query
sites
Q5EXK5

K

R

R

Q

F

W

F

R

I

V

M

I

V

I

L

C

F

F

I

L

T

V

V

V

V

M

I

L

N

D

Y

G

L

F

D

D

R

T

S

A

N

A

L

I

S

G

I

F

A

I

A

A

P

P

A

D

L

I

T

R

S

S

D

H

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W

G

Q

I

L

D

T

P

A

I

G

H

D

V

L

G

A

L

P

I

L

F

F

S

G

A

A

F

G

G

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W

L

T

G

Y

L

A

T

A

A

M

G

Q

A

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L

G

C

G

G

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P

L

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S

D
|
D

R

R

V

F

P

G

P

R

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K

I

R

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Y

I

S

E

V

F

A

C

L

V

L

A

L

L

W

F

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V

L

A

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T

S

V

L

M

L

L

S

G

A

F

F

A

A

G

P

S

D

F

L

I

Q

A

M

L

L

F

V

V

F

L

L

R

R

M

F

A

L

V

T

G

G

A

L

L

G

E

L

A

G

P

G

A

A

Y

M

P

P

I

N

N

T

S

I

R

T

V

M

V

T

T

S

T

E

W

Y

F

L

P

P

E

A

R

R

E

R

R

R

A

G

T

A

A

L

I

V

G

T

F

L

Y

M

T

F

S

C

G

G

Q

F

F

T

V

L

G

G

L

S

A

A

F

F

L

G

T

G

P

V

V

V

L

S

A

A

W

Q

L

L

Q

V

H

P

E

L

F

I

G

G

W

W

H

H

M

G

V

I

F

L

V

V

T

L

T

G

G

G

A

V

V

L

G

P

I

L

V

I

W

L

A

A

V

V

I

A

W

L

Y

V

A

P

V

L

Y

L

R

P

E

E

-

S

P

P

R

R

D

W

F

Q

K

I

G

R

A

R

N

Q

D

L

A

P

E

Q

I

A

D

T

L

I

I

A

R

R

E

T

G

V

G

G

G

A

L

I

V

T

D

G

I

-

Q

-

A

-

E

-

Q

-

A

E

R

R

V

Y

K

N

A

D

K

T

F

H

S

F

W

W

T

L

D

D

L

E

G

P

I

A

V

A

L

G

T

A

K

K

R

G

K

S

L

I

W

S

G

Q

I

L

Y

F

L

A

G

G

-

R

Q

Q

F

L

C

A

L

I

N

T

S

L

T

M

L

L

W

W

-

V

F

F

L

M

-

S

-

L

-

I

-

Y

-

L

-

S

T

S

W

W

F

M

P

P

T

T

Y

-

L

L

V

L

K

N

Y

H

R

R

G

G

M

I

D

D

F

L

D82 (= D81) mutation to A: Loss of activity.

Sites not aligning to the query:

311 V→W: Loss of activity.
314 D→A: Loss of activity.

P0AGC0 Hexose-6-phosphate:phosphate antiporter from Escherichia coli (strain K12) (see paper)
26% identity, 29% coverage: 24:151/436 of query aligns to 33:165/463 of P0AGC0

query
sites
P0AGC0

V

L

L

V

F

F

I

I

T

G

V

Y

V

L

I

T

N

M

Y

Y

L

L

D

I

R

R

S

K

N

N

L

F

S

N

I

I

A

A

A

Q

P

N

A

D

L

M

T

I

S

S

D

T

L

Y

G

G

I

L

D

S

P

M

I

T

H

Q

V

L

G

G

L

M

I

I

F

G

S

L

A

G

F

F

G

S

W

I

T

T

Y

Y

A

G

A

V

M

G

Q

K

I

T

P

L

G

V

G

S

W

Y

L

Y

V

A

D

D

R

G

V

K

P

N

P

T

R

K

I

Q

L

F

Y

L

S

P

V

F

A

M

L

L

L

I

L

L

W

-

S

-

V

S

A

A

T

I

V
x
C

M

M

L

L

G

G

F

F

A

S

G

A

S

S

F

M

-

G

-

S

-

G

-

S

I

V

A

S

L

L

F

F

V

L

L

M

-

I

-

A

-

F

R

Y

M

A

A

L

V

S

G

G

A

F

L

F

E

Q

A

S

P

T

A

G

Y

G

P

S

I
x
C

N

S

S

Y

R

S

V

T

V

I

T

T

T

K

W

W

F

T

P

P

E

R

R

R

E

K

R

R

A

G

T

T

A

F

I

L

G

G

F

F

Y

W

T

N

C108 (≠ V101) mutation to S: No change in activity.
C143 (≠ I129) mutation to S: 30% of wild-type sugar phosphate transport activity.

Sites not aligning to the query:

265 C→S: No change in activity.
331 C→S: No change in activity.
436 C→S: No change in activity.
438 C→S: No change in activity.

Q51330 Oxalate:formate antiporter; OFA; Oxalate:formate antiport protein; Oxalate:formate exchange protein from Oxalobacter formigenes (see 4 papers)
25% identity, 38% coverage: 2:166/436 of query aligns to 3:162/418 of Q51330

query
sites
Q51330

Q

N

P

P

Q

Q

T

T

L

-

T

-

G

G

Q

Q

A

S

S

T

L

G

V

L

T

L

P

G

S

N

R

R

K

W

R

F

F

Y

F

L

I

V

M

L

V

A

L

V

L

L

F

L

I

M

T
x
C

V

M

V

I

I

S

N

G

Y

V

L

-

D

-

R

Q

S

Y

N

S

L

W

S

T

I

L

A

Y

A

A

P

N

A

P

L

V

T

K

S

D

D

N

L

L

G

G

I

V

D

S

P

L

I

A

H

A

V

V

G
x
Q

L

T

I

A

F
|
F

S

T

A

L

F

S

G

Q

W

V

T

I

Y
x
Q

A

A

A

G

M
x
S

Q

Q

I

P

P

G

G

G

W

Y

L

F

V

V

D

D

R

K

V

F

P

G

P

P

R

R

I

I

-

P

-

L

L

M

Y

F

S

G

V

G

A

A

L

M

L

V

L

L

W

-

S

-

V

A

A

G

T

W

V

T

M

F

L

M

G

G

F

M

A

V

G

D

S

S

F

V

I

P

A

A

L

L

F

Y

V

A

L

L

R

Y

M

T

A

L

V

A

G

G

A

A

L

G

E

V

A

G

P

I

A

V

Y

Y

P

G

I

I

N

A

S

M

R

N

V

T

V

A

T

N

T

R

W

W

F

F

P

P

E

D

R

K

E

-

R

R

A

G

T

L

A

A

I

S

G

G

F

F

Y

T

T

A

S

A

G

G

Q

Y

F

G

V

L

G

G

L

V

A

L

F

P

L

F

T

L

P

P

V

L

L

I

A

S

C28 (≠ T29) mutation to G: Slight decrease in activity; when associated with A-271.
Q56 (≠ G59) mutation to C: Residual activity.
F59 (= F62) mutation to C: Loss of activity.
Q66 (≠ Y69) mutation to C: Residual activity.
S69 (≠ M72) mutation to C: Residual activity.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
271 C→A: Slight decrease in activity; when associated with G-28.
349 G→C: Loss of activity.
355 mutation K->C,G,Q,T: Loss of activity.; K→R: Residual activity.
362 G→C: Residual activity.
363 G→C: Residual activity.

Query Sequence

>PfGW456L13_2130 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2130
MQPQTLTGQASLVTPSRKRFFIMVLLFITVVINYLDRSNLSIAAPALTSDLGIDPIHVGL
IFSAFGWTYAAMQIPGGWLVDRVPPRILYSVALLLWSVATVMLGFAGSFIALFVLRMAVG
ALEAPAYPINSRVVTTWFPERERATAIGFYTSGQFVGLAFLTPVLAWLQHEFGWHMVFVT
TGAVGIVWAVIWYAVYREPRDFKGANDAEIDLIREGGGLVDIQAEQARVKAKFSWTDLGI
VLTKRKLWGIYLGQFCLNSTLWFFLTWFPTYLVKYRGMDFIKSGLLASLPFLAAFIGVLC
SGFFSDFLIRRGYTVGFARKLPIISGLLISTSIIGANFVESTPLVIAFLALAFFGNGLAS
ITWSLVSTLAPARLLGLTGGVFNFIGNLSAIATPIVIGFLATGDSFAPAITYISVLALIG
ALSYILLVGKVERIKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory