SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_2157 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2157 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09110 3-ketoacyl-CoA thiolase, peroxisomal; Acetyl-CoA C-myristoyltransferase; Acetyl-CoA acyltransferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase; EC 2.3.1.16; EC 2.3.1.155; EC 2.3.1.9 from Homo sapiens (Human) (see 3 papers)
43% identity, 99% coverage: 3:391/394 of query aligns to 37:420/424 of P09110

query
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P09110

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V387 (= V359) to A: in dbSNP:rs2229528

Sites not aligning to the query:

1:26 PTS2-type peroxisomal targeting signal
5 mutation Q->D,K,L: Does not affect localization to peroxisomes.
6 V→D: Abolished localization to peroxisomes.; V→K: Does not affect localization to peroxisomes.
7 mutation V->D,K: Abolished localization to peroxisomes.
8 mutation L->D,K: Does not affect localization to peroxisomes.
9 mutation G->D,R,L: Does not affect localization to peroxisomes.
10 H→E: In S3E mutant; Abolished localization to peroxisomes.

2d3tC Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form v (see paper)
40% identity, 99% coverage: 2:393/394 of query aligns to 5:390/390 of 2d3tC

query
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2d3tC

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active site: C94 (= C90), H346 (= H349), C376 (= C379), G378 (= G381)
binding acetyl coenzyme *a: C94 (= C90), M129 (≠ K125), M150 (= M148), H176 (≠ Q174), R214 (= R221), L222 (= L229), L225 (= L232), A238 (= A244), G239 (= G245), S242 (= S248), I244 (≠ L250), M283 (= M289), A313 (= A319), F314 (= F320), H346 (= H349), C376 (= C379)

5bz4K Crystal structure of a t1-like thiolase (coa-complex) from mycobacterium smegmatis (see paper)
41% identity, 100% coverage: 2:394/394 of query aligns to 1:399/400 of 5bz4K

query
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5bz4K

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active site: C87 (= C90), H354 (= H349), C384 (= C379), G386 (= G381)
binding coenzyme a: C87 (= C90), R146 (≠ K138), M160 (= M148), L161 (≠ G149), R220 (= R221), L228 (= L229), L231 (= L232), A246 (= A244), G247 (= G245), S250 (= S248), Q252 (≠ L250), M291 (= M289), A321 (= A319), F322 (= F320), H354 (= H349)

P45359 Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; CaTHL; EC 2.3.1.9 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) (see paper)
37% identity, 99% coverage: 1:391/394 of query aligns to 1:390/392 of P45359

query
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P45359

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R

K

E

E

V

V

I

I

V

A

D

S

S

A

V

V

R

R

T

T

G

A

L

I

A

G

K

-

S

S

F

Y

R

G

G

K

K

S

F

L

N

K

M

D

T

V

R

P

P

A

D

V

D

D

M

L

A

G

A

A

H

T

C

A

V

I

D

K

A

E

L

A

L

V

A

K

R

K

T

A

G

G

I

I

D

K

P

P

A

E

S

D

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

N

G

V

S

L

N

Q

E

A

G

G

A

L

Q

-

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

S

V

F

L

K

S

A

R

G

L

L

G

P

I
x
V

G

E

T

I

A

P

G

A

M

M

T

T

L

I

N

N

R

K

F

V

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

T

I

V

A
x
S

I

L

A

A

N

Q

Q

I

I

I

A

K

S

A

G

G

C

D

S

A

D

D

I

V

I

I

V

I

A

A

G

G

V

M

E

E

S

N

I

M

S

S

L

R

T

A

M

P

K

Y

S

L

V

A

N

N

T

N

D

A

-

R

-

W

-

G

-

Y

-

R

-

M

-

G

-

N

-

A

N

K

L

F

I

V

N

D

P

E

L

M

L

I

K

T

E

D

Q

G

V

L

P

W

G

D

I

A

Y

F

-
x
N

-

D

F

Y

P

H

M

M

G

G

Q

I

T

T

A

A

E

E

I

N

V

I

A

A

R

E

R

R

Y

W

N

N

V

I

S

S

R

R

Q

E

E

E

Q

Q

D

D

L

E

Y

F

A

A

L

L

Q

A

S

S

Q

Q

Q

K

R

K

T

A

A

E

E

E

A

A

Q

I

A

K

A

S

G

G

L

Q

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

V

S

V

V

I

K

K

Y

G

R

R

V

-

E

-

D

-

K

-

A

-

T

K

G

G

Q

E

A

T

Q

-

I

-

L

-

D

-

G

-

I

V

V

V

D

D

R

T

D

D

D

E

C

H

N

P

R

R

P

F

D

G

T

S

T

T

L

I

E

E

S

G

L

L

T

A

G

K

L

L

K

K

P

P

V

A

F

F

A

K

E

K

D

D

G

G

S

T

V

V

T

T

A

A

G

G

N

N

S

A

S

S

Q

G

L

L

S

N

D

D

G

C

A

A

S

A

M

V

T

L

L

V

V

I

M

M

S

S

L

A

E

E

K

K

A

A

L

K

E

E

L

L

G

G

L

V

K

K

P

P

K

L

A

A

F

K

F

I

R

V

G

S

F

Y

T
x
G

V
x
S

A

A

G

G

C

V

A

D

P

P

D
x
A

E

I

M

M

G

G

I

Y

G

G

P

P

V

F

F

Y

S

A

V

T

P

K

K

A

L

A

L

I

K

E

A

K

K

A

G

G

L

W

R

T

I

V

A

D

D

E

I

L

D

D

L

L

W

I

E

E

L

S

N

N

E

E

A

A

F

F

A

A

S

A

Q

Q

C

S

L

L

Y

A

S

V

R

A

N

K

R

D

L

L

E

K

I

F

D

D

N

M

A

N

R

K

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

L

G

G

H

H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

V

H

T

L

L

V

V

R

H

E

A

L

M

Q

Q

R

K

R

R

N

D

L

A

R

K

Y

K

G

G

I

L

V

A

T

T

M

L

C
|
C

V

I

G

G

M

Q

G

G

A

T

T
x
A

G

I

L

L

F

L

E

E

V77 (≠ I79) mutation to Q: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Y-153 and K-286.
C88 (= C90) modified: Disulfide link with 378, In inhibited form
S96 (≠ A98) binding
N153 (vs. gap) mutation to Y: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and K-286.
GS 279:280 (≠ TV 280:281) binding
A286 (≠ D287) mutation to K: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and Y-153.
C378 (= C379) modified: Disulfide link with 88, In inhibited form
A386 (≠ T387) binding

4xl4A Crystal structure of thiolase from clostridium acetobutylicum in complex with coa (see paper)
37% identity, 99% coverage: 1:391/394 of query aligns to 1:390/392 of 4xl4A

query
sites
4xl4A

M

M

R

K

E

E

V

V

I

I

V

A

D

S

S

A

V

V

R

R

T

T

G

A

L

I

A

G

K

-

S

S

F

Y

R

G

G

K

K

S

F

L

N

K

M

D

T

V

R

P

P

A

D

V

D

D

M

L

A

G

A

A

H

T

C

A

V

I

D

K

A

E

L

A

L

V

A

K

R

K

T

A

G

G

I

I

D

K

P

P

A

E

S

D

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

N

G

V

S

L

N

Q

E

A

G

G

A

L

Q

-

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

S

V

F

L

K

S

A

R

G

L

L

G

P

I

V

G

E

T

I

A

P

G

A

M

M

T

T

L

I

N

N

R

K

F

V

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

T

I

V

A

S

I

L

A

A

N

Q

Q

I

I

I

A

K

S

A

G

G

C

D

S

A

D

D

I

V

I

I

V

I

A

A

G

G

V

M

E

E

S

N

I

M

S

S

L

R

T

A

M

P

K

Y

S

L

V

A

N

N

T

N

D

A

-

R

-

W

-

G

-

Y

-

R

-

M

-

G

-

N

-

A

N

K

L

F

I

V

N

D

P

E

L

M

L

I

K

T

E

D

Q

G

V
x
L

P

W

G

D

I

A

Y

F

-

N

-

D

F

Y

P
x
H

M
|
M

G

G

Q

I

T

T

A

A

E

E

I

N

V

I

A

A

R

E

R

R

Y

W

N

N

V

I

S

S

R

R

Q

E

E

E

Q

Q

D

D

L

E

Y

F

A

A

L

L

Q

A

S

S

Q

Q

Q

K

R

K

T

A

A

E

E

E

A

A

Q

I

A

K

A

S

G

G

L

Q

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

V

S

V

V

I

K

K

Y

G

R

R

V

-

E

-

D

-

K

-

A

-

T

K

G

G

Q

E

A

T

Q

-

I

-

L

-

D

-

G

-

I

V

V

V

D

D

R

T

D

D

D

E

C

H

N

P

R
|
R

P

F

D

G

T

S

T

T

L

I

E

E

S

G

L
|
L

T

A

G

K

L
|
L

K

K

P

P

V

A

F
|
F

A

K

E

K

D

D

G

G

S

T

V

V

T

T

A
|
A

G
|
G

N

N

S

A

S
|
S

Q
x
G

L
|
L

S

N

D

D

G

C

A

A

S

A

M

V

T

L

L

V

V

I

M

M

S

S

L

A

E

E

K

K

A

A

L

K

E

E

L

L

G

G

L

V

K

K

P

P

K

L

A

A

F

K

F

I

R

V

G

S

F

Y

T

G

V

S

A

A

G

G

C

V

A

D

P

P

D

A

E

I

M

M

G

G

I

Y

G

G

P

P

V

F

F

Y

S

A

V

T

P

K

K

A

L

A

L

I

K

E

A

K

K

A

G

G

L

W

R

T

I

V

A

D

D

E

I

L

D

D

L

L

W

I

E

E

L

S

N

N

E

E

A
|
A

F
|
F

A

A

S

A

Q

Q

C

S

L

L

Y

A

S

V

R

A

N

K

R

D

L

L

E

K

I

F

D

D

N

M

A

N

R

K

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

L

G

G

H
|
H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

V

H

T

L

L

V

V

R

H

E

A

L

M

Q

Q

R

K

R

R

N

D

L

A

R

K

Y

K

G

G

I

L

V

A

T

T

M

L

C
x
S

V

I

G
|
G

G

G

M

Q

G

G

A

T

T

A

G

I

L

L

F

L

E

E

active site: C88 (= C90), H348 (= H349), S378 (≠ C379), G380 (= G381)
binding coenzyme a: L148 (≠ V141), H156 (≠ P147), M157 (= M148), R220 (= R221), L228 (= L229), L231 (= L232), F235 (= F236), A243 (= A244), G244 (= G245), S247 (= S248), G248 (≠ Q249), L249 (= L250), A318 (= A319), F319 (= F320), H348 (= H349)

2vu2A Biosynthetic thiolase from z. Ramigera. Complex with s-pantetheine-11- pivalate. (see paper)
37% identity, 99% coverage: 4:393/394 of query aligns to 2:389/389 of 2vu2A

query
sites
2vu2A

V

I

V

V

I

I

V

A

D

S

S

A

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

T

R

P

P

A

D

H

D

E

M

L

A

G

A

A

H

T

C

V

V

I

D

S

A

A

L

V

L

L

A

E

R

R

T

A

G

G

I

V

D

A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

T

G

A

M

W

T

G

L

M

N

N

R

Q

F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

G

A

M

N

Q

Q

Q

I

I

A

A

S

T

G

G

C

D

S

A

D

S

I

I

V

V

A

A

G

G

V

M

E

E

S

S

I

M

S

S

L

M

T

A

M

P

K

H

S

C

V

A

N

H

T

L

D

R

-

G

-

V

-

K

-

M

-

G

-

D

-

F

N

K

L

M

I

I

N

D

P

T

L

M

L

I

K

K

E

D

Q

G

V
x
L

P

T

G

D

I

A

Y

F

F

Y

-

G

-

Y

P
x
H

M
|
M

G

G

Q

T

T

T

A

A

E

E

I

N

V

V

A

A

R

K

R

Q

Y

W

N

Q

V

L

S

S

R

R

Q

D

E

E

Q

Q

D

D

L

A

Y

F

A

A

L

V

Q

A

S

S

Q

Q

Q

N

R

K

T

A

A

E

E

A

A

A

Q

Q

A

K

A

D

G

G

L

R

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

F

S

I

V

V

K

K

Y

G

R

R

V

K

E

G

D

D

K

I

A

-

T

-

G

-

Q

-

A

-

Q

-

I

-

L

-

D

-

G

-

I

T

V

V

D

D

R

A

D

D

D

E

C

Y

N

I

R

R

P

H

D

G

T

A

T

T

L

L

E

D

S

S

L

M

T

A

G

K

L

L

K

R

P

P

V

A

F
|
F

A

D

E

K

D

E

G

G

S

T

V

V

T

T

A
|
A

G

G

N

N

S

A

S
|
S

Q
x
G

L
|
L

S

N

D

D

G

G

A

A

S

A

M

A

T

A

L

L

V

L

M

M

S

S

L

E

E

A

K

E

A

A

L

S

E

R

L

R

G

G

L

I

K

Q

P

P

K

L

A

G

F

R

F

I

R

V

G

S

F

W

T

A

V

T

A

V

G

G

C

V

A

D

P

P

D

K

E

V

M

M

G

G

I

T

G

G

P

P

V

I

F

P

S

A

V

S

P

R

K

K

L

A

L

L

K

E

A

R

K

A

G

G

L

W

R

K

I

I

A

G

D

D

I

L

D

D

L

L

W

V

E

E

L

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

Q

Q

C

A

L

C

Y

A

S

V

R

N

N

K

R

D

L

L

E

G

I

W

D

D

N

P

A

S

R

I

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

I

G

G

H
|
H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

N

H

T

L

L

V

L

R

F

E

E

L

M

Q

K

R

R

N

G

L

A

R

R

Y

K

G

G

I

L

V

A

T

T

M

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

A

V

T

A

G

M

L

C

F

I

E

E

A

S

V

L

active site: C86 (= C90), H345 (= H349), C375 (= C379), G377 (= G381)
binding (3R)-3-hydroxy-2,2-dimethyl-4-oxo-4-({3-oxo-3-[(2-sulfanylethyl)amino]propyl}amino)butyl 2,2-dimethylpropanoate: C86 (= C90), L145 (≠ V141), H153 (≠ P147), M154 (= M148), F232 (= F236), A240 (= A244), S244 (= S248), G245 (≠ Q249), L246 (= L250), A315 (= A319), F316 (= F320), H345 (= H349)

1dm3A Acetylated biosynthetic thiolase from zoogloea ramigera in complex with acetyl-coa (see paper)
37% identity, 99% coverage: 4:393/394 of query aligns to 2:389/389 of 1dm3A

query
sites
1dm3A

V

I

V

V

I

I

V

A

D

S

S

A

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

T

R

P

P

A

D

H

D

E

M

L

A

G

A

A

H

T

C

V

V

I

D

S

A

A

L

V

L

L

A

E

R

R

T

A

G

G

I

V

D

A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

T

G

A

M

W

T

G

L

M

N

N

R

Q

F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

G

A

M

N

Q

Q

Q

I

I

A

A

S

T

G

G

C

D

S

A

D

S

I

I

V

V

A

A

G

G

V

M

E

E

S

S

I

M

S

S

L

M

T

A

M

P

K

H

S

C

V

A

N

H

T

L

D

R

-

G

-

V

-

K

-

M

-

G

-

D

-

F

N

K

L

M

I

I

N

D

P

T

L

M

L

I

K

K

E

D

Q

G

V
x
L

P

T

G

D

I

A

Y

F

F

Y

-

G

-

Y

P
x
H

M
|
M

G

G

Q

T

T

T

A

A

E

E

I

N

V

V

A

A

R

K

R

Q

Y

W

N

Q

V

L

S

S

R

R

Q

D

E

E

Q

Q

D

D

L

A

Y

F

A

A

L

V

Q

A

S

S

Q

Q

Q

N

R

K

T

A

A

E

E

A

A

A

Q

Q

A

K

A

D

G

G

L

R

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

F

S

I

V

V

K

K

Y

G

R

R

V

K

E

G

D

D

K

I

A

-

T

-

G

-

Q

-

A

-

Q

-

I

-

L

-

D

-

G

-

I

T

V

V

D

D

R

A

D

D

D

E

C

Y

N

I

R
|
R

P

H

D

G

T

A

T

T

L

L

E

D

S
|
S

L
x
M

T

A

G

K

L
|
L

K

R

P

P

V

A

F
|
F

A

D

E

K

D

E

G

G

S

T

V

V

T

T

A
|
A

G
|
G

N

N

S
x
A

S
|
S

Q
x
G

L
|
L

S

N

D

D

G

G

A

A

S

A

M

A

T

A

L

L

V

L

M

M

S

S

L

E

E

A

K

E

A

A

L

S

E

R

L

R

G

G

L

I

K

Q

P

P

K

L

A

G

F

R

F

I

R

V

G

S

F

W

T

A

V

T

A

V

G

G

C

V

A

D

P

P

D

K

E

V

M
|
M

G

G

I

T

G

G

P

P

V

I

F

P

S

A

V

S

P

R

K

K

L

A

L

L

K

E

A

R

K

A

G

G

L

W

R

K

I

I

A

G

D

D

I

L

D

D

L

L

W

V

E

E

L

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

Q

Q

C

A

L

C

Y

A

S

V

R

N

N

K

R

D

L

L

E

G

I

W

D

D

N

P

A

S

R

I

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

I

G

G

H
|
H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

N

H

T

L

L

V

L

R

F

E

E

L

M

Q

K

R

R

N

G

L

A

R

R

Y

K

G

G

I

L

V

A

T

T

M

L

C
|
C

V
x
I

G
|
G

G

G

M

M

G

G

A

V

T

A

G

M

L

C

F

I

E

E

A

S

V

L

active site: C86 (= C90), H345 (= H349), C375 (= C379), G377 (= G381)
binding acetyl coenzyme *a: C86 (= C90), L145 (≠ V141), H153 (≠ P147), M154 (= M148), R217 (= R221), S224 (= S228), M225 (≠ L229), L228 (= L232), F232 (= F236), A240 (= A244), G241 (= G245), A243 (≠ S247), S244 (= S248), G245 (≠ Q249), L246 (= L250), M285 (= M289), A315 (= A319), F316 (= F320), H345 (= H349), C375 (= C379), I376 (≠ V380)

1dlvA Biosynthetic thiolase from zoogloea ramigera in complex with coa (see paper)
37% identity, 99% coverage: 4:393/394 of query aligns to 2:389/389 of 1dlvA

query
sites
1dlvA

V

I

V

V

I

I

V

A

D

S

S

A

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

T

R

P

P

A

D

H

D

E

M

L

A

G

A

A

H

T

C

V

V

I

D

S

A

A

L

V

L

L

A

E

R

R

T

A

G

G

I

V

D

A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

T

G

A

M

W

T

G

L

M

N

N

R

Q

F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

G

A

M

N

Q

Q

Q

I

I

A

A

S

T

G

G

C

D

S

A

D

S

I

I

V

V

A

A

G

G

V

M

E

E

S

S

I

M

S

S

L

M

T

A

M

P

K

H

S

C

V

A

N

H

T

L

D

R

-

G

-

V

-

K

-

M

-

G

-

D

-

F

N

K

L

M

I

I

N

D

P

T

L

M

L

I

K

K

E

D

Q

G

V
x
L

P

T

G

D

I

A

Y

F

F

Y

-

G

-

Y

P
x
H

M
|
M

G

G

Q

T

T

T

A

A

E

E

I

N

V

V

A

A

R

K

R

Q

Y

W

N

Q

V

L

S

S

R

R

Q

D

E

E

Q

Q

D

D

L

A

Y

F

A

A

L

V

Q

A

S

S

Q

Q

Q

N

R

K

T

A

A

E

E

A

A

A

Q

Q

A

K

A

D

G

G

L

R

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

F

S

I

V

V

K

K

Y

G

R

R

V

K

E

G

D

D

K

I

A

-

T

-

G

-

Q

-

A

-

Q

-

I

-

L

-

D

-

G

-

I

T

V

V

D

D

R

A

D

D

D

E

C

Y

N

I

R
|
R

P

H

D

G

T

A

T

T

L

L

E

D

S
|
S

L
x
M

T

A

G

K

L
|
L

K

R

P

P

V

A

F
|
F

A

D

E

K

D

E

G

G

S

T

V

V

T

T

A
|
A

G
|
G

N

N

S

A

S
|
S

Q
x
G

L
|
L

S

N

D

D

G

G

A

A

S

A

M

A

T

A

L

L

V

L

M

M

S

S

L

E

E

A

K

E

A

A

L

S

E

R

L

R

G

G

L

I

K

Q

P

P

K

L

A

G

F

R

F

I

R

V

G

S

F

W

T

A

V

T

A

V

G

G

C

V

A

D

P

P

D

K

E

V

M

M

G

G

I

T

G

G

P

P

V

I

F

P

S

A

V

S

P

R

K

K

L

A

L

L

K

E

A

R

K

A

G

G

L

W

R

K

I

I

A

G

D

D

I

L

D

D

L

L

W

V

E

E

L

A

N

N

E

E

A

A

F
|
F

A

A

S

A

Q

Q

C

A

L

C

Y

A

S

V

R

N

N

K

R

D

L

L

E

G

I

W

D

D

N

P

A

S

R

I

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

I

G

G

H
|
H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

N

H

T

L

L

V

L

R

F

E

E

L

M

Q

K

R

R

N

G

L

A

R

R

Y

K

G

G

I

L

V

A

T

T

M

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

A

V

T

A

G

M

L

C

F

I

E

E

A

S

V

L

active site: C86 (= C90), H345 (= H349), C375 (= C379), G377 (= G381)
binding coenzyme a: C86 (= C90), L145 (≠ V141), H153 (≠ P147), M154 (= M148), R217 (= R221), S224 (= S228), M225 (≠ L229), L228 (= L232), F232 (= F236), A240 (= A244), G241 (= G245), S244 (= S248), G245 (≠ Q249), L246 (= L250), F316 (= F320), H345 (= H349)

2vu1A Biosynthetic thiolase from z. Ramigera. Complex of with o-pantheteine- 11-pivalate. (see paper)
37% identity, 99% coverage: 4:393/394 of query aligns to 4:391/391 of 2vu1A

query
sites
2vu1A

V

I

V

V

I

I

V

A

D

S

S

A

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

T

R

P

P

A

D

H

D

E

M

L

A

G

A

A

H

T

C

V

V

I

D

S

A

A

L

V

L

L

A

E

R

R

T

A

G

G

I

V

D

A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

T

G

A

M

W

T

G

L

M

N

N

R

Q

F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

G

A

M

N

Q

Q

Q

I

I

A

A

S

T

G

G

C

D

S

A

D

S

I

I

V

V

A

A

G

G

V

M

E

E

S

S

I

M

S

S

L

M

T

A

M

P

K

H

S

C

V

A

N

H

T

L

D

R

-

G

-

V

-

K

-

M

-

G

-

D

-

F

N

K

L

M

I

I

N

D

P

T

L

M

L

I

K

K

E

D

Q

G

V
x
L

P

T

G

D

I

A

Y

F

F

Y

-

G

-

Y

P
x
H

M

M

G

G

Q

T

T

T

A

A

E

E

I

N

V

V

A

A

R

K

R

Q

Y

W

N

Q

V

L

S

S

R

R

Q

D

E

E

Q

Q

D

D

L

A

Y

F

A

A

L

V

Q

A

S

S

Q

Q

Q

N

R

K

T

A

A

E

E

A

A

A

Q

Q

A

K

A

D

G

G

L

R

F

F

T

K

D

D

E

E

I

I

V

V

P

P

M

F

S

I

V

V

K

K

Y

G

R

R

V

K

E

G

D

D

K

I

A

-

T

-

G

-

Q

-

A

-

Q

-

I

-

L

-

D

-

G

-

I

T

V

V

D

D

R

A

D

D

D

E

C

Y

N

I

R

R

P

H

D

G

T

A

T

T

L

L

E

D

S

S

L

M

T

A

G

K

L

L

K

R

P

P

V

A

F
|
F

A

D

E

K

D

E

G

G

S

T

V

V

T

T

A
|
A

G

G

N

N

S

A

S
|
S

Q
x
G

L
|
L

S

N

D

D

G

G

A

A

S

A

M

A

T

A

L

L

V

L

M

M

S

S

L

E

E

A

K

E

A

A

L

S

E

R

L

R

G

G

L

I

K

Q

P

P

K

L

A

G

F

R

F

I

R

V

G

S

F

W

T

A

V

T

A

V

G

G

C

V

A

D

P

P

D

K

E

V

M

M

G

G

I

T

G

G

P

P

V

I

F

P

S

A

V

S

P

R

K

K

L

A

L

L

K

E

A

R

K

A

G

G

L

W

R

K

I

I

A

G

D

D

I

L

D

D

L

L

W

V

E

E

L

A

N

N

E

E

A
|
A

F
|
F

A

A

S

A

Q

Q

C

A

L

C

Y

A

S

V

R

N

N

K

R

D

L

L

E

G

I

W

D

D

N

P

A

S

R

I

Y

V

N

N

V

V

N

N

G

G

S

A

I

I

S

A

I

I

G

G

H
|
H

P

P

F

I

G

G

M

A

T

S

G

G

S

A

R

R

Q

I

V

L

G

N

H

T

L

L

V

L

R

F

E

E

L

M

Q

K

R

R

N

G

L

A

R

R

Y

K

G

G

I

L

V

A

T

T

M

L

C
|
C

V

I

G
|
G

G

G

M

M

G

G

A

V

T

A

G

M

L

C

F

I

E

E

A

S

V

L

active site: C88 (= C90), H347 (= H349), C377 (= C379), G379 (= G381)
binding pantothenyl-aminoethanol-11-pivalic acid: L147 (≠ V141), H155 (≠ P147), F234 (= F236), A242 (= A244), S246 (= S248), G247 (≠ Q249), L248 (= L250), A317 (= A319), F318 (= F320), H347 (= H349)

1ou6A Biosynthetic thiolase from zoogloea ramigera in complex with acetyl-o- pantetheine-11-pivalate
37% identity, 99% coverage: 4:393/394 of query aligns to 5:392/392 of 1ou6A

query
sites
1ou6A

V

I

V

V

I

I

V

A

D

S

S

A

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

T

R

P

P

A

D

H

D

E

M

L

A

G

A

A

H

T

C

V

V

I

D

S

A

A

L

V

L

L

A

E

R

R

T

A

G

G

I

V

D

A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

T

G

A

M

W

T

G

L

M

N

N

R

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F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

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N

Q

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I

A

A

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G

G

C

D

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A

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I

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A

A

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E

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I

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M

T

A

M

P

K

H

S

C

V

A

N

H

T

L

D

R

-

G

-

V

-

K

-

M

-

G

-

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-

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N

D

P

T

L

M

L
x
I

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K

E

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x
L

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A

Y

F

F

Y

-

G

-

Y

P
x
H

M
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M

G

G

Q

T

T

T

A

A

E

E

I

N

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A

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R

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R

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A

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-

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x
A

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|
F

A

D

E

K

D

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G

G

S

T

V

V

T

T

A
|
A

G

G

N

N

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S

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x
G

L
|
L

S

N

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D

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G

A

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A

T

A

L

L

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P

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M

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G

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A

L

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I

A

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A

N

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E

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A
|
A

F
|
F

A

A

S

A

Q

Q

C

A

L

C

Y

A

S

V

R

N

N

K

R

D

L

L

E

G

I

W

D

D

N

P

A

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R

I

Y

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N

N

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N

N

G

G

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A

I

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A

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H
|
H

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I

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A

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C

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G

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G

G

A

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F

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active site: C89 (= C90), H348 (= H349), C378 (= C379), G380 (= G381)
binding pantothenyl-aminoethanol-acetate pivalic acid: I144 (≠ L137), L148 (≠ V141), H156 (≠ P147), M157 (= M148), A234 (≠ V235), F235 (= F236), A243 (= A244), S247 (= S248), G248 (≠ Q249), L249 (= L250), A318 (= A319), F319 (= F320), H348 (= H349)

P07097 Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; Beta-ketothiolase; EC 2.3.1.9 from Shinella zoogloeoides (Crabtreella saccharophila) (see 2 papers)
37% identity, 99% coverage: 5:393/394 of query aligns to 6:392/392 of P07097

query
sites
P07097

V

I

I

V

V

I

D

A

S

S

V

A

R

R

T

T

G

A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

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P

A

D

H

D

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A

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A

A

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V

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A

A

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L

L

A

E

R

R

T

A

G

G

I

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A

P

A

A

G

S

E

V

V

E

N

D

E

C

V

I

I

V

L

G

G

A

Q

G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F
x
Q

N

N

I

P

G

A

R

R

N

Q

V

A

A

A

V

M

L

K

S

A

R

G

L

V

G

P

I

Q

G

E

T

A

A

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G

A

M

W

T

G

L

M

N

N

R

Q

F

L

C
|
C

S

G

S

S

G

G

L

L

Q

R

A

A

I

V

A

A

I

L

A

G

A

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N

Q

Q

Q

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I

A

A

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T

G

G

C

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A

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S

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I

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A

A

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G

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E

E

S

S

I

M

S

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L

M

T

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M

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A

N

H

T

L

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A

-

G

-

V

-

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M

-

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-

D

-

F

N

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N

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D

I

A

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Y

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M

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G

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T

T

A

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A

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R

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Q

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A

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F

A

A

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A

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A

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A

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F

A

D

E

K

D

E

G

G

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V

V

T

T

A

A

G

G

N

N

S

A

S

S

Q

G

L

L

S

N

D

D

G

G

A

A

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A

M

A

T

A

L

L

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L

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M

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A

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G

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P

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V

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S

F

W

T

A

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T

A

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G

C

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A

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P

D

K

E

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M

G

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A

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S

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K

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A

L

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A

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R

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I

I

A

G

D

D

I

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D

D

L

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W

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E

E

L

A

N

N

E

E

A

A

F

F

A

A

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A

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Q

C

A

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C

Y

A

S

V

R

N

N

K

R

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L

L

E

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I

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D

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N

P

A

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R

I

Y

V

N

N

V

V

N

N

G

G

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A

I

I

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A

I

I

G

G

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H

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G

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R

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G

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A

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C
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C

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M

G

G

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C

F

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E

E

A

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L

Q64 (≠ F65) mutation to A: Slightly lower activity.
C89 (= C90) mutation to A: Loss of activity.
C378 (= C379) mutation to G: Loss of activity.

2wkuA Biosynthetic thiolase from z. Ramigera. The n316h mutant. (see paper)
37% identity, 99% coverage: 4:393/394 of query aligns to 2:389/389 of 2wkuA

query
sites
2wkuA

V

I

V

V

I

I

V
x
A

D
x
S

S
x
A

V

A

R

R

T

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A

L

V

A

G

K

-

S

S

F

F

R

N

G

G

K

A

F

F

N

A

M

N

T

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P

A

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H

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M

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A

G

A

A

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A

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L

L

A

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R

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A

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G

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A

P

A

A

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S

E

V

V

E

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D

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I

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G

G

A

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G

V

S

L

N

P

E

A

G

G

A

-

Q

E

G

G

F

Q

N

N

I

P

G

A

R

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N

Q

V

A

A

A

V

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S

A

R

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L

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G

P

I

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G

E

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A

A

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A

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W

T

G

L

M

N

N

R

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L