SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_2714 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2714 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
53% identity, 98% coverage: 7:455/457 of query aligns to 1:396/396 of 5dm3C

query
sites
5dm3C

S

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F

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active site: E115 (= E144), E117 (= E146), E162 (= E207), E169 (= E214), H218 (= H263), R286 (= R336), E303 (= E353), R305 (= R355)
binding adenosine-5'-diphosphate: R173 (= R218), C174 (≠ Y219), H220 (= H265), S222 (= S267), R301 (= R351)

5dm3A Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
51% identity, 98% coverage: 6:455/457 of query aligns to 2:374/374 of 5dm3A

query
sites
5dm3A

L

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active site: E107 (= E144), E109 (= E146), E146 (= E207), E150 (= E214), H199 (= H263), R265 (= R336), E282 (= E353), R284 (= R355)
binding adenosine-5'-diphosphate: I103 (≠ M140), E141 (= E202), R154 (= R218), C155 (≠ Y219), H201 (= H265), S203 (= S267), R280 (= R351)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
32% identity, 81% coverage: 81:449/457 of query aligns to 64:431/439 of 7tdpA

query
sites
7tdpA

G

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T

H

D

Q

W

W

E

E

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A142), E127 (= E144), E179 (= E202), D193 (≠ N216), Y196 (= Y219), N242 (≠ H265), S244 (= S267), R316 (= R341), R326 (= R351)
binding magnesium ion: E127 (= E144), E127 (= E144), E129 (= E146), E184 (= E207), E191 (= E214), E191 (= E214), H240 (= H263), E328 (= E353)
binding l-methionine-s-sulfoximine phosphate: E127 (= E144), E129 (= E146), E184 (= E207), E191 (= E214), G236 (= G259), H240 (= H263), R293 (= R318), E299 (≠ F324), R311 (= R336), R330 (= R355)

7tfaB Glutamine synthetase (see paper)
32% identity, 81% coverage: 81:449/457 of query aligns to 64:433/441 of 7tfaB

query
sites
7tfaB

G

S

D

D

F

M

V

Y

L

L

K

Y

P

P

D

D

M

L

S

D

T

T

L

W

R

V

V

F

P

P

W

W

L

V

E

T

C

S

-

D

-

R

T

V

A

A

L

R

V

L

L

I

C

C

D

D

V

I

L

-

D

-

H

-

H

Y

R

K

Q

P

D

D

-

G

-

S

-

P

L

F

P

A

H

G

S

D

P

P

R

R

A

G

I

I

L

L

K

K

K

R

Q

V

V

L

E

K

R

E

L

A

R

E

E

E

R

L

G

G

Y

Y

T

T

G

S

M

M

-

N

F

V

A

G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

T

D

D

E

E

S

K

Y

G

E

D

A

P

I

T

H

T

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

D

T

Q

A

G

G

G

H
x
Y

Y

F

-

D

I

L

E

A

D

P

Y

M

N

D

I

L

L

G

Q

E

T

N

T

C

R

R

E

R

E

E

P

I

V

V

L

L

R

K

A

-

I

-

R

-

K

-

H

-

L

L

Q

E

A

E

S

M

G

G

I

F

P

E

V

I

E

E

N

A

S

S

K

H

G

H

E
|
E

W
x
V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N

D

I

F

R

K

Y

Y

A

A

D

D

A

A

L

V

T

K

M

A

A

A

D

D

H

Q

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

Q

Q

H

G

G

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D

G

A

V

A
x
N

G
|
G

S

S

S

G

S

M

H
|
H

I

C

H

N

N

Q

S

S

L

L

W

F

D

K

K

D

N

N

A

E

K

N

K

-

P

-

L

V

F

F

F

Y

D

D

A

E

K

T

A

D

E

E

F

L

G

G

M

L

S

S

K

Q

L

T

M

A

R

R

S

H

W

Y

V

M

A

A

G

G

Q

I

L

L

K

K

Y

H

A

A

N

R

D

A

I

M

T

A

C

A

F

I

L

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

K

K

R
|
R

F

L

Q

V

A

P

G
|
G

T
x
Y

F
x
E

A

A

P

P

T

C

R

Y

A

V

V

A

W

W

S

S

R

A

D

S

N

N

R
|
R

T

S

A

P

G

M

F

I

R

R

L

I

C

P

A

A

E

S

G

R

S

G

K

L

A

S

I

T

R

R

I

V

E
|
E

C

V

R

R

I

N

G

P

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

A

A

V

L

M

I

L

A

R

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

R

K

Q

L

M

D

A

L

L

A

P

P

A

P

P

F

I

E

D

G

R

D

N

A

I

Y

Y

-

V

-

M

-

S

-

E

-

R

L

I

D

E

E

E

H

G

L

I

P

P

E

S

V

L

S

P

K

A

T

D

L

L

R

K

E

E

A

A

S

L

A

S

A

E

L

L

K

I

T

R

S

S

G

E

M

V

L

I

R

S

K

D

A

A

F

L

G

G

D

D

E

H

V

A

V

L

D

A

H

Y

Y

F

V

Y

H

E

T

L

A

K

E

E

W

I

E

E

Q

W

K

D

E
x
M

Y

Y

D

R

R

T

R

Q

I

V

T

H

D

Q

W

W

E

E

binding glutamine: E131 (= E146), Y153 (≠ H170), E186 (= E207), G238 (= G259), H242 (= H263), R295 (= R318), E301 (≠ F324)
binding magnesium ion: E129 (= E144), E131 (= E146), E186 (= E207), E193 (= E214), H242 (= H263), E330 (= E353)
binding : V187 (≠ W208), N237 (≠ A258), G299 (= G322), Y300 (≠ T323), R313 (= R336), M424 (≠ E440)

Sites not aligning to the query:

binding : 58, 60

7tf6A Glutamine synthetase (see paper)
31% identity, 82% coverage: 82:457/457 of query aligns to 65:438/438 of 7tf6A

query
sites
7tf6A

D

D

F

M

V

Y

L

L

K

H

P

P

D

D

M

L

S

D

T

T

L

W

R

V

R

I

V

F

P

P

W

W

L

G

E

K

C

V

T

A

A

R

L

L

V

I

L

-

C

C

D

D

V

V

L

-

D

-

H

-

H

Y

R

K

Q

T

D

D

-

G

-

T

-

P

L

F

P

E

H

G

S

D

P

P

R

R

A

A

I

N

L

L

K

K

K

R

Q

V

V

L

E

K

R

E

L

M

R

E

E

D

R

L

G

G

Y

F

T

T

G

D

M

F

-

N

F

L

A

G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

L

D

D

E

E

S

K

Y

G

E

E

A

P

I

T

H

L

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

-

T

D

A

D

G

G

H

G

Y

Y

I

-

E

-

D

-

Y

F

N

D

I

L

L

A

Q

P

T

T

T

D

R

L

E

G

E

E

P

N

V

C

L

R

R

R

A

D

I

I

R

V

K

L

H

E

L

L

Q

E

A

D

S

M

G

G

I

F

P

D

V

I

E

E

N

A

S

S

K

H

G

H

E
|
E

W

V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N

D

I

F

R

K

Y

Y

A

A

D

D

A

A

L

V

T

T

M

A

A

C

D

D

H

N

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

K

Q

H

G

N

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D
x
G

A

V

A
x
N

G
|
G

S

S

S

G

S

M

H
|
H

I

F

H

N

N

V

S

S

L

L

W

F

D

-

K

-

N

K

A

G

K

K

K

E

P

N

L

A

F

F

D

D

A

P

K

N

A

T

E

E

F

M

G

G

M

L

S

T

K

E

L

T

M

A

R

Y

S

Q

W

F

V

T

A

A

G

G

Q

V

L

L

K

K

Y

N

A

A

N

R

D

G

I

F

T

T

C

A

F

V

L

C

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

K

K

R
|
R

F

L

Q

V

A

P

G
|
G

T
x
Y

F
x
E

A

A

P

P

T

C

R

Y

A

I

V

A

W

W

S

S

R

G

D

K

N

N

R
|
R

T

S

A

P

G

L

F

I

R

R

L

V

C

P

A

S

E

S

G

R

S

G

K

L

A

S

I

T

R

R

I

I

E
|
E

C

V

R

R

I

S

G

V

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

M

A

A

F

L

A

A

L

I

I

L

A

E

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

K

K

L

L

D

K

L

V

A

P

P

E

P

P

F

V

E

N

G

Q

D

N

A

I

Y
|
Y

L

E

D

M

E

N

H

R

-

E

-

R

-

E

-

A

-

V

-

G

L

I

P

Q

E

D

V

L

S

P

K

S

T

T

L

L

R

Y

E

T

A

A

S

L

A

K

A

A

L

M

K

R

T

E

S

N

G

E

M

V

L

I

R

K

K

K

A

A

F

L

G

G

D

N

E

H

V

I

V

Y

D

N

H

Q

Y

F

V

I

H

N

T

S

A

K

E

S

W

I

E

E

Q

W

K

D

E
x
Y

Y

Y

D

R

R

T

R

Q

I

V

T

S

D

E

W

W

E

E

L

R

Q

D

R
x
Q

G

Y

F

M

E

K

R
x
Q

Y

Y

binding glutamine: E128 (= E146), E183 (= E207), G235 (= G259), H239 (= H263), R292 (= R318), E298 (≠ F324)
binding magnesium ion: E126 (= E144), E128 (= E146), E183 (= E207), E190 (= E214), H239 (= H263), E327 (= E353)
binding : G232 (≠ D256), N234 (≠ A258), G296 (= G322), Y297 (≠ T323), R310 (= R336), Y367 (= Y393), Y421 (≠ E440), Q433 (≠ R452), Q437 (≠ R456)

Sites not aligning to the query:

binding : 58, 60

7tdvC Glutamine synthetase (see paper)
30% identity, 82% coverage: 82:457/457 of query aligns to 66:443/443 of 7tdvC

query
sites
7tdvC

D

D

F

M

V

Y

L

L

K

H

P

P

D

D

M

L

S

D

T

T

L

W

R

V

R

I

V

F

P

P

W

W

L

T

E

A

C

G

T

Q

A

G

L

K

V

V

L

A

C

R

D

L

V

I

L

C

D

D

H

V

H

Y

H

K

R

T

Q

D

D

G

L

T

P

P

H

F

-

E

-

G

S

D

P

P

R

R

A

A

I

N

L

L

K

K

K

R

Q

V

V

L

E

K

R

E

L

M

R

E

E

D

R

L

G

G

Y

F

T

T

G

D

M

F

-

N

F

L

A
x
G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

L

D

D

E

E

S

K

Y

G

E

E

A

P

I

T

H

L

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

-

T

D

A

D

G

G

H

G

Y

Y

I

-

E

-

D

-

Y

F

N

D

I

L

L

A

Q

P

T

T

T

D

R

L

E

G

E

E

P

N

V

C

L

R

R

R

A

D

I

I

R

V

K

L

H

E

L

L

Q

E

A

D

S

M

G

G

I

F

P

D

V

I

E
|
E

N

A

S

S

K

H

G

H

E
|
E

W

V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N
x
D

I
x
F

R
x
K

Y
|
Y

A

A

D

D

A

A

L

V

T

T

M

A

A

C

D

D

H

N

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

K

Q

H

G

N

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D

G

A

V

A

N

G
|
G

S

S

S

G

S

M

H
|
H

I

F

H
x
N

N
x
V

S
|
S

L

L

W

F

D

-

K

-

N

K

A

G

K

K

K

E

P

N

L

A

F

F

D

D

A

P

K

N

A

T

E

E

F

M

G

G

M

L

S

T

K

E

L

T

M

A

R

Y

S

Q

W

F

V

T

A

A

G

G

Q

V

L

L

K

K

Y

N

A

A

N

R

D

G

I

F

T

T

C

A

F

V

L

C

A

N

P

P

Y

L

I

V

N

N

S

S

Y

Y

K

K

R
|
R

F

L

Q

V

A

P

G

G

T

Y

F
x
E

A

A

P

P

T

C

R

Y

A

I

V

A

W

W

S

S

R

G

D

K

N

N

R
|
R

T

S

A

P

G

L

F

I

R
|
R

L

V

C

P

A

S

E

S

G

R

S

G

K

L

A
x
S

I

T

R
|
R

I

I

E
|
E

C

V

R

R

I

S

G

V

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

M

A

A

F

L

A

A

L

I

I

L

A

E

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

K

K

L

L

D

K

L

V

A

P

P

E

P

P

F

V

E

N

G

Q

D

N

A

I

Y

Y

L

E

D

M

E

N

H

R

-

E

-

R

-

E

-

A

-

V

-

G

L

I

P

Q

E

D

V

L

S

P

K

S

T

T

L

L

R

Y

E

T

A

A

S

L

A

K

A

A

L

M

K

R

T

E

S

N

G

E

M

V

L

I

R

K

K

K

A

A

F

L

G

G

D

N

E

H

V

I

V

Y

D

N

H

Q

Y

F

V

I

H

N

T

S

A

K

E

S

W

I

E

E

Q

W

K

D

E

Y

Y

Y

D

R

R

T

R

Q

I

V

T

S

D

E

W

W

E

E

L

R

Q

D

R

Q

G

Y

F

M

E

K

R

Q

Y

Y

binding adenosine-5'-diphosphate: G129 (≠ A142), E131 (= E144), E183 (= E202), D197 (≠ N216), F198 (≠ I217), K199 (≠ R218), Y200 (= Y219), N246 (≠ H265), V247 (≠ N266), S248 (= S267), R320 (= R341), S328 (≠ A349), R330 (= R351)
binding magnesium ion: E131 (= E144), E131 (= E144), E133 (= E146), E188 (= E207), E195 (= E214), E195 (= E214), H244 (= H263), E332 (= E353)
binding l-methionine-s-sulfoximine phosphate: E131 (= E144), E133 (= E146), E188 (= E207), E195 (= E214), G240 (= G259), H244 (= H263), R297 (= R318), E303 (≠ F324), R315 (= R336)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
30% identity, 82% coverage: 82:457/457 of query aligns to 66:443/443 of 4lnkA

query
sites
4lnkA

D

D

F

M

V

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

L

F

R

V

R

I

V

F

P

P

W

W

L

T

E

A

C

E

T

K

A

G

L

K

V

V

L

A

C

R

D

F

V

I

L

C

D

D

H

I

H

Y

H

N

R

P

Q

D

D

G

L

T

P

P

H

F

S

E

-

G

-

D

P

P

R

R

A

N

I

N

L

L

K

K

K

R

Q

I

V

L

E

K

R

E

L

M

R

E

E

D

R

L

G

G

Y

F

T

S

G

D

M

F

-

N

F

L

A

G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

L

D

D

E

E

S

K

Y

G

E

E

A

P

I

T

H

L

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

-

T

D

A

K

G

G

H

G

Y

Y

I

-

E

-

D

-

Y

F

N

D

I

L

L

A

Q

P

T

T

T

D

R

L

E

G

E

E

P

N

V

C

L

R

R

R

A

D

I

I

R

V

K

L

H

E

L

L

Q

E

A

E

S

M

G

G

I

F

P

E

V

I

E

E

N

A

S

S

K

H

G

H

E
|
E

W
x
V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N

D

I
x
F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

H

D

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

K

Q

H

G

G

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D

G

A

V

A
x
N

G
|
G

S

S

S
x
G

S

M

H
|
H

I

C

H
x
N

N

L

S
|
S

L

L

W

F

D

K

K

N

N

G

A

V

K

N

K

A

P

-

L

-

F

F

D

D

A

E

K

N

A

A

E

D

F

L

G

Q

M

L

S

S

K

E

L

T

M

A

R

K

S

H

W

F

V

I

A

A

G

G

Q

I

L

V

K

K

Y

H

A

A

N

T

D

S

I

F

T

T

C

A

F

V

L

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

K

K

R

R

F

L

Q

V

A

P

G

G

T

Y

F
x
E

A

A

P

P

T

C

R

Y

A

V

V

A

W

W

S

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

F

I

R

R

L

I

C

P

A

A

E
x
S

G

R

S

G

K

I

A
x
S

I

T

R
|
R

I

V

E
|
E

C

V

R
|
R

I

S

G

V

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

S

A

V

L

L

I

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

K

K

L

L

D

E

L

A

A

P

P

A

P

P

F

I

E

D

G

R

D

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

L

M

D

E

E

N

H

G

L

I

P

V

E

D

V

L

S

P

K

A

T

T

L

L

R

A

E

E

A

A

S

L

A

E

L

F

K

K

T

S

S

N

G

E

M

V

L

M

R

V

K

K

A

A

F

L

G

G

D

E

E

H

V

L

V

F

D

E

H

H

Y

F

V

I

H

E

T

A

A

K

E

E

W

I

E

E

Q

W

K

D

E

M

Y

F

D

R

R

T

R

Q

I

V

T

H

D

P

W

W

E

E

L

R

Q

E

R

Q

G

Y

F

M

E

S

R

Q

Y

Y

active site: E131 (= E144), E133 (= E146), E188 (= E207), E195 (= E214), H244 (= H263), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: F198 (≠ I217), Y200 (= Y219), N246 (≠ H265), S248 (= S267), S324 (≠ E345), S328 (≠ A349), R330 (= R351)
binding glutamic acid: E133 (= E146), E188 (= E207), V189 (≠ W208), N239 (≠ A258), G240 (= G259), G242 (≠ S261), E303 (≠ F324)
binding magnesium ion: E131 (= E144), E188 (= E207), E195 (= E214), H244 (= H263), E332 (= E353)

Sites not aligning to the query:

active site: 52
binding adenosine-5'-diphosphate: 43, 50

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
30% identity, 82% coverage: 82:457/457 of query aligns to 66:443/443 of 4lniA

query
sites
4lniA

D

D

F

M

V

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

L

F

R

V

R

I

V

F

P

P

W

W

L

T

E

A

C

E

T

K

A

G

L

K

V

V

L

A

C

R

D

F

V

I

L

C

D

D

H

I

H

Y

H

N

R

P

Q

D

D

G

L

T

P

P

H

F

S

E

-

G

-

D

P

P

R

R

A

N

I

N

L

L

K

K

K

R

Q

I

V

L

E

K

R

E

L

M

R

E

E

D

R

L

G

G

Y

F

T

S

G

D

M

F

-

N

F

L

A

G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

L

D

D

E

E

S

K

Y

G

E

E

A

P

I

T

H

L

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

-

T

D

A

K

G

G

H

G

Y

Y

I

-

E

-

D

-

Y

F

N

D

I

L

L

A

Q

P

T

T

T

D

R

L

E

G

E

E

P

N

V

C

L

R

R

R

A

D

I

I

R

V

K

L

H

E

L

L

Q

E

A

E

S

M

G

G

I

F

P

E

V

I

E
|
E

N

A

S

S

K

H

G

H

E
|
E

W

V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N
x
D

I

F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

H

D

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

K

Q

H

G

G

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D

G

A

V

A

N

G

G

S

S

S

G

S

M

H
|
H

I

C

H
x
N

N

L

S
|
S

L

L

W

F

D

K

K

N

N

G

A

V

K

N

K

A

P

-

L

-

F

F

D

D

A

E

K

N

A

A

E

D

F

L

G

Q

M

L

S

S

K

E

L

T

M

A

R

K

S

H

W

F

V

I

A

A

G

G

Q

I

L

V

K

K

Y

H

A

A

N

T

D

S

I

F

T

T

C

A

F

V

L

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

K

K

R
|
R

F

L

Q

V

A

P

G

G

T

Y

F
x
E

A

A

P

P

T

C

R

Y

A

V

V

A

W

W

S

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

F

I

R
|
R

L

I

C

P

A

A

E

S

G

R

S

G

K

I

A

S

I

T

R
|
R

I

V

E
|
E

C

V

R
|
R

I

S

G

V

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

S

A

V

L

L

I

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

K

K

L

L

D

E

L

A

A

P

P

A

P

P

F

I

E

D

G

R

D

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

L

M

D

E

E

N

H

G

L

I

P

V

E

D

V

L

S

P

K

A

T

T

L

L

R

A

E

E

A

A

S

L

A

E

L

F

K

K

T

S

S

N

G

E

M

V

L

M

R

V

K

K

A

A

F

L

G

G

D

E

E

H

V

L

V

F

D

E

H

H

Y

F

V

I

H

E

T

A

A

K

E

E

W

I

E

E

Q

W

K

D

E

M

Y

F

D

R

R

T

R

Q

I

V

T

H

D

P

W

W

E

E

L

R

Q

E

R

Q

G

Y

F

M

E

S

R

Q

Y

Y

active site: E131 (= E144), E133 (= E146), E188 (= E207), E195 (= E214), H244 (= H263), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: E131 (= E144), E183 (= E202), D197 (≠ N216), Y200 (= Y219), N246 (≠ H265), S248 (= S267), R320 (= R341), R330 (= R351)
binding magnesium ion: E131 (= E144), E131 (= E144), E133 (= E146), E188 (= E207), E195 (= E214), E195 (= E214), H244 (= H263), E332 (= E353)
binding l-methionine-s-sulfoximine phosphate: E133 (= E146), E188 (= E207), H244 (= H263), R297 (= R318), E303 (≠ F324), R315 (= R336), R334 (= R355)

Sites not aligning to the query:

active site: 52

4s0rD Structure of gs-tnra complex (see paper)
30% identity, 82% coverage: 82:457/457 of query aligns to 70:447/447 of 4s0rD

query
sites
4s0rD

D

D

F

M

V

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

L

F

R

V

R

I

V

F

P

P

W

W

L

T

E

A

C

E

T

K

A

G

L

K

V

V

L

A

C

R

D

F

V

I

L

C

D

D

H

I

H

Y

H

N

R

P

Q

D

D

G

L

T

P

P

H

F

S

E

-

G

-

D

P

P

R

R

A

N

I

N

L

L

K

K

K

R

Q

I

V

L

E

K

R

E

L

M

R

E

E

D

R

L

G

G

Y

F

T

S

G

D

M

F

-

N

F

L

A

G

S

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

F

F

-

K

-

L

D

D

E

E

S

K

Y

G

E

E

A

P

I

T

H

L

K

E

R

L

N

N

Y

-

H

-

K

-

P

-

K

-

T

D

A

K

G

G

H

G

Y

Y

I

-

E

-

D

-

Y

F

N
x
D

I

L

L

A

Q

P

T

T

T

D

R

L

E

G

E

E

P

N

V

C

L

R

R

R

A

D

I

I

R

V

K

L

H

E

L

L

Q

E

A

E

S

M

G

G

I

F

P

E

V

I

E

E

N

A

S

S

K

H

G

H

E
|
E

W

V

G

A

P

P

G

G

Q

Q

E

H

E
|
E

I

I

N

D

I

F

R

K

Y

Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

H

D

H

I

V

Q

I

T

I

F

K

K

H

L

A

V

C

V

K

K

E

T

I

I

A

A

Q

R

L

K

Q

H

G

G

K

L

A

H

I

A

T

T

F

F

M

M

A

P

K

K

W

P

R

L

Y

F

D
x
G

A
x
V

A
x
N

G

G

S

S

S

G

S

M

H
|
H

I

C

H

N

N

L

S

S

L

L

W

F

D

K

K

N

N

G

A

V

K

N

K

A

P

-

L

-

F

F

D

D

A

E

K

N

A

A

E

D

F

L

G

Q

M

L

S

S

K

E

L

T

M

A

R

K

S

H

W

F

V

I

A

A

G

G

Q

I

L

V

K

K

Y

H

A

A

N

T

D

S

I

F

T

T

C

A

F

V

L

T

A

N

P

P

Y

T

I

V

N

N

S

S

Y

Y

K

K

R
|
R

F

L

Q

V

A

P

G
|
G

T
x
Y

F
x
E

A

A

P

P

T

C

R

Y

A

V

V

A

W

W

S

S

R

A

D

Q

N

N

R
|
R

T

S

A

P

G

L

F

I

R

R

L

I

C

P

A

A

E

S

G

R

S

G

K

I

A

S

I

T

R

R

I

V

E
|
E

C

V

R
|
R

I

S

G

V

G

D

A

P

D

A

L

A

N

N

P

P

Y

Y

L

L

A

A

F

L

A

S

A

V

L

L

I

L

A

A

G

G

L

L

A

D

G

G

I

I

D

K

E

N

K

K

L

L

D

E

L

A

A

P

P

A

P

P

F

I

E

D

G

R

D

N

A

I

Y
|
Y

-

V

-

M

-

S

-

K

-

E

-

R

L

M

D

E

E

N

H

G

L

I

P

V

E

D

V

L

S

P

K

A

T

T

L

L

R

A

E

E

A

A

S

L

A

E

L

F

K

K

T

S

S

N

G

E

M

V

L

M

R

V

K

K

A

A

F

L

G

G

D

E

E

H

V

L

V

F

D

E

H
|
H

Y

F

V

I

H

E

T

A

A

K

E

E

W
x
I

E

E

Q

W

K

D

E
x
M

Y

F

D

R

R

T

R

Q

I

V

T

H

D

P

W

W

E

E

L

R

Q

E

R

Q

G

Y

F

M

E

S

R

Q

Y

Y

active site: E135 (= E144), E137 (= E146), E192 (= E207), E199 (= E214), H248 (= H263), R319 (= R336), E336 (= E353), R338 (= R355)
binding glutamine: E137 (= E146), E192 (= E207), R301 (= R318), E307 (≠ F324)
binding magnesium ion: E135 (= E144), E135 (= E144), E199 (= E214), H248 (= H263), H248 (= H263), E336 (= E353), H419 (= H429)
binding : D161 (≠ N176), G241 (≠ D256), V242 (≠ A257), N243 (≠ A258), G305 (= G322), Y306 (≠ T323), Y376 (= Y393), I426 (≠ W436), M430 (≠ E440)

Sites not aligning to the query:

active site: 56
binding magnesium ion: 66
binding : 63, 64, 65, 66

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
30% identity, 82% coverage: 82:457/457 of query aligns to 67:444/444 of P12425

query
sites
P12425

D

D

F

M

V

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

L

F

R

V

R

I

V

F

P

P

W

W

L

T

E

A

C

E

T

K

A

G

L

K

V

V

L

A

C

R

D

F

V

I

L

C

D

D

H

I

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E132 (= E144) binding
E134 (= E146) binding
E189 (= E207) binding
V190 (≠ W208) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E214) binding
G241 (= G259) binding
H245 (= H263) binding
G302 (= G322) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ F324) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P326) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E353) binding
E424 (= E437) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
59 G→R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
62 Important for inhibition by glutamine; R→A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
30% identity, 81% coverage: 82:451/457 of query aligns to 66:441/446 of A0R083

query
sites
A0R083

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