SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_3207 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3207 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6lpiB Crystal structure of ahas holo-enzyme (see paper)
32% identity, 99% coverage: 4:530/535 of query aligns to 4:518/539 of 6lpiB

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6lpiB

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active site: I27 (= I27), G29 (= G29), G30 (≠ V30), S31 (≠ H31), I32 (≠ T32), E53 (= E52), C76 (≠ I75), F115 (≠ L116), Q116 (≠ H117), E117 (= E118), K165 (≠ L167), M256 (≠ S256), A283 (≠ D283), V375 (≠ M383), G401 (= G409), M403 (≠ L411), D428 (= D436), N455 (= N464), A457 (= A466), L458 (= L467), L460 (≠ Q469), V461 (≠ I470), Q464 (≠ D473)
binding flavin-adenine dinucleotide: R155 (= R157), G212 (= G214), G213 (= G215), G214 (= G216), T236 (≠ S238), L237 (≠ V239), M238 (≠ A240), M253 (vs. gap), L254 (vs. gap), G255 (≠ S255), M256 (≠ S256), H257 (≠ L257), G276 (= G276), A277 (≠ T277), R278 (≠ E278), D280 (≠ A280), R282 (≠ T282), A283 (≠ D283), D300 (= D299), I301 (= I300), D302 (= D301), E305 (≠ K304), A318 (≠ G317), D319 (= D318), V320 (≠ A319), Q379 (≠ A387), M380 (≠ Y388), G398 (≠ T406), G399 (= G407)
binding magnesium ion: D428 (= D436), N455 (= N464)
binding thiamine diphosphate: P28 (= P28), E53 (= E52), C76 (≠ I75), P79 (= P78), G80 (= G79), N83 (= N82), V375 (≠ M383), G376 (≠ T384), Q377 (= Q385), H378 (≠ L386), G401 (= G409), T402 (= T410), M403 (≠ L411), G427 (= G435), D428 (= D436), G429 (= G437), S430 (≠ G438), M433 (≠ Y441), N455 (= N464), A457 (= A466), L458 (= L467), G459 (= G468), L460 (≠ Q469), V461 (≠ I470)

1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 98% coverage: 6:530/535 of query aligns to 7:539/582 of 1t9dB

query
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1t9dB

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active site: Y28 (≠ I27), G30 (= G29), G31 (≠ V30), A32 (≠ H31), I33 (≠ T32), E54 (= E52), T77 (≠ I75), F116 (≠ L116), Q117 (≠ H117), E118 (= E118), K166 (≠ L167), R213 (vs. gap), M249 (≠ S255), V276 (vs. gap), V392 (≠ M383), L417 (≠ Y408), G418 (= G409), M420 (≠ L411), D445 (= D436), N472 (= N464), E474 (≠ A466), Q475 (≠ L467), M477 (≠ Q469), V478 (≠ I470), W481 (vs. gap), L503 (= L494), G508 (= G499), L509 (≠ C500)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (≠ V30), A32 (≠ H31), V106 (≠ N104), P107 (≠ H105), A110 (≠ S108), A115 (≠ S115), F116 (≠ L116), K166 (≠ L167), M249 (≠ S255), D274 (= D281), R275 (vs. gap), M477 (≠ Q469), V478 (≠ I470), W481 (vs. gap)
binding flavin-adenine dinucleotide: D95 (= D93), R156 (= R157), G202 (= G214), A203 (≠ G215), G204 (= G216), N207 (= N219), T229 (≠ S238), L230 (≠ V239), Q231 (≠ A240), M246 (vs. gap), L247 (≠ A253), G248 (= G254), M249 (≠ S255), H250 (≠ S256), G269 (= G276), A270 (≠ T277), R271 (≠ E278), D273 (≠ A280), R275 (vs. gap), V276 (vs. gap), F301 (≠ V298), E302 (≠ D299), V303 (≠ I300), S304 (≠ D301), N307 (≠ K304), G320 (= G317), D321 (= D318), A322 (= A319), Q396 (≠ A387), M397 (≠ Y388), G415 (≠ T406), G416 (= G407)
binding magnesium ion: D445 (= D436), N472 (= N464), E474 (≠ A466)
binding 2,5-dimethyl-pyrimidin-4-ylamine: Y28 (≠ I27), P29 (= P28), E54 (= E52), T77 (≠ I75), P80 (= P78), Q117 (≠ H117), G418 (= G409), T419 (= T410), M420 (≠ L411), M450 (≠ Y441), Q475 (≠ L467)

Sites not aligning to the query:

active site: 542

1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 98% coverage: 6:530/535 of query aligns to 8:540/583 of 1t9bA

query
sites
1t9bA

L

L

T

T

G

G

Q

Q

A

I

L

F

V

N

R

E

L

M

A

S

N

R

Y

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
I

L

L

E

P

L

V

Y

Y

R

D

G

A

L

I

P

H

G

N

S

S

-

D

G

K

I

F

R

N

H

F

V

V

L

L

T

P

R

K

H

H

E
|
E

Q

Q

G

G

A

A

S

G

F

H

M

M

A

A

D

E

G

G

Y

Y

A

A

R

R

V

A

S

S

G

G

K

K

P

P

G

G

V

V

C

V

F

L

V

V

I
x
T

T

S

G

G

P

P

G

G

V

A

T

T

N

N

A

V

T

T

G

P

I

M

G

A

Q

D

A

A

Y

F

A

A

D
|
D

S

G

I

I

P

P

M

M

L

V

V

V

I

F

S

T

S

G

V

Q

N
x
V

H
x
P

S

T

A

S

S

A

L

I

G

G

K

T

G

-

W

-

G

D

S

A

L
x
F

H
x
Q

E
|
E

C

A

Q

-

D

D

Q

V

R

V

A

G

M

I

T

S

A

R

P

S

I

C

T

T

A

K

F

W

S

N

A

V

V

M

A

V

L

K

T

S

A

V

E

E

D

E

L

L

P

P

E

L

L

R

I

I

A

N

R

E

A

A

Y

F

A

E

V

I

F

A

D

T

S

S

E

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

S

D

V

L

P

P

L
x
K

D

D

V

V

L

T

S

A

A

A

P

-

I

-

A

-

R

-

D

-

W

-

S

-

N

-

E

-

V

I

V

L

R

R

R

N

P

P

G

-

R

-

G

I

P

P

A

F

S

V

T

M

T

Q

A

S

I

I

D

N

Q

K

A

A

V

A

A

D

K

L

L

I

N

N

A

L

A

A

K

K

R

K

P

P

M

V

I

L

I

Y

A

V

G
|
G

G
x
A

G
|
G

A

I

L

L

N
|
N

A

H

T

A

S

D

E

G

-

P

-
x
R

-

L

L

L

Q

K

E

E

V

L

S

S

T

D

R

R

L

A

A

Q

A

I

P

P

L

V

F

T

T

T

S
x
T

V
x
L

A
x
Q

G

G

K

L

G

G

L

S

L

F

P

D

P

Q

D

E

A

D

P

P

L

K

N

S

-

L

-

D

-
x
M

A
x
L

G
|
G

S
x
M

S
x
H

L

G

C

C

V

A

E

T

P

A

G

-

W

-

N

N

L

L

-

A

I

V

A

Q

E

N

A

A

D

D

V

L

V

I

L

I

A

A

V

V

G
|
G

T
x
A

E
x
R

M

F

A
x
D

D
|
D

-
x
R

-
x
V

-

T

-

G

-

N

-

I

T

S

D

K

F

F

W

A

R

P

E

E

R

A

L

R

P

R

L

A

-

A

-

E

-

G

N

R

G

G

E

G

L

I

R

H

V
x
F

D
x
E

I
x
V

D
x
S

P

P

R

K

K
x
N

F

I

N

N

D

K

F

V

Y

V

P

Q

C

T

A

Q

V

I

A

A

L

V

H

E

G
|
G

D
|
D

A
|
A

Q

T

T

N

L

L

S

G

G

K

L

M

E

S

R

K

L

I

P

-

T

F

D

P

V

V

R

K

E

E

A

R

S

S

A

E

A

W

I

F

A

A

S

Q

V

I

A

N

A

K

L

W

R

K

K

K

A

E

V

Y

K

P

A

Y

S

A

H

Y

-

M

-

E

G

T

P

P

L

G

Q

S

S

K

I

I

H

K

-

P

Q

Q

S

T

I

V

-

I

-

K

L

L

D

S

R

K

I

V

A

A

A

N

E

D

L

T

P

G

D

R

N

H

A

V

F

I

I

V

S

T

T

T

D

G

M
x
V

T

G

Q

Q

L

H

A
x
Q

Y
x
M

T

W

G

A

N

A

Y

Q

A

H

F

W

D

T

S

W

L

R

A

N

I

P

R

H

S

T

W

F

L

I

H

T

P

S

T
x
G

G
|
G

Y
x
L

G
|
G

T

T

L
x
M

G

G

Y

Y

G

G

L

L

P

P

A

A

G

A

I

I

G

G

A

A

K

Q

F

V

G

A

A

K

P

P

Q

E

R

S

P

L

G

V

L

I

V

D

L

I

V

D

G

G

D
|
D

G

A

G

S

F

F

L

N

Y

M

T

T

A

L

Q

T

E

E

L

L

A

S

T

S

S

A

V

V

E

Q

E

A

L

-

D

G

S

T

P

P

L

V

V

K

V

I

L

L

L

I

W

L

N

N

N
|
N

D

E

A
x
E

L
x
Q

G

G

Q
x
M

I
x
V

R

T

D

Q

-
x
W

D

Q

M

S

L

L

G

F

L

Y

D

E

I

H

E

R

P

Y

I

S

G

H

V

T

L

H

P

Q

R

L

N

N

P

P

D

D

F

F

A

I

A

K

L
|
L

A

A

R

E

A

A

F

M

G
|
G

C
x
L

T

K

V

G

T

L

Q

R

P

V

Q

K

S

K

L

Q

A

E

E

E

L

L

Q

D

T

A

D

K

L

L

R

K

H

E

G

F

F

V

K

S

R

T

N

K

G

G

V

P

T

V

L

L

I

L

E

E

L

V

K

E

active site: Y29 (≠ I27), G31 (= G29), G32 (≠ V30), A33 (≠ H31), I34 (≠ T32), E55 (= E52), T78 (≠ I75), F117 (≠ L116), Q118 (≠ H117), E119 (= E118), K167 (≠ L167), R214 (vs. gap), M250 (≠ S255), V277 (vs. gap), V393 (≠ M383), L418 (≠ Y408), G419 (= G409), M421 (≠ L411), D446 (= D436), N473 (= N464), E475 (≠ A466), Q476 (≠ L467), M478 (≠ Q469), V479 (≠ I470), W482 (vs. gap), L504 (= L494), G509 (= G499), L510 (≠ C500)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: G32 (≠ V30), A33 (≠ H31), V107 (≠ N104), P108 (≠ H105), F117 (≠ L116), K167 (≠ L167), M250 (≠ S255), D275 (= D281), R276 (vs. gap), M478 (≠ Q469), V479 (≠ I470), W482 (vs. gap)
binding flavin-adenine dinucleotide: D96 (= D93), R157 (= R157), G203 (= G214), A204 (≠ G215), G205 (= G216), N208 (= N219), T230 (≠ S238), L231 (≠ V239), Q232 (≠ A240), M247 (vs. gap), L248 (≠ A253), G249 (= G254), M250 (≠ S255), H251 (≠ S256), G270 (= G276), A271 (≠ T277), R272 (≠ E278), D274 (≠ A280), R276 (vs. gap), V277 (vs. gap), F302 (≠ V298), E303 (≠ D299), V304 (≠ I300), S305 (≠ D301), N308 (≠ K304), G321 (= G317), D322 (= D318), A323 (= A319), Q397 (≠ A387), M398 (≠ Y388), G416 (≠ T406), G417 (= G407)
binding magnesium ion: D446 (= D436), N473 (= N464), E475 (≠ A466)

Sites not aligning to the query:

active site: 543

5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 98% coverage: 6:530/535 of query aligns to 8:548/591 of 5wkcA

query
sites
5wkcA

L

L

T

T

G

G

Q

Q

A

I

L

F

V

N

R

E

L

M

A

S

N

R

Y

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

I
x
Y

P
|
P

G
|
G

V
x
G

H
x
A

T
x
I

L
|
L

E

P

L

V

Y

Y

R

D

G

A

L

I

P

H

G

N

S

S

-

D

G

K

I

F

R

N

H

F

V

V

L

L

T

P

R

K

H

H

E
|
E

Q

Q

G

G

A

A

S

G

F

H

M

M

A

A

D

E

G

G

Y

Y

A

A

R

R

V

A

S

S

G

G

K

K

P

P

G

G

V

V

C

V

F

L

V

V

I
x
T

T

S

G

G

P
|
P

G

G

V

A

T

T

N

N

A

V

T

T

G

P

I

M

G

A

Q

D

A

A

Y

F

A

A

D
|
D

S

G

I

I

P

P

M

M

L

V

V

V

I

F

S

T

S

G

V

Q

N
x
V

H
x
P

S

T

A

S

S

A

L

I

G

G

K

T

G

-

W

-

G

D

S

A

L
x
F

H
x
Q

E
|
E

C

A

Q

-

D

D

Q

V

R

V

A

G

M

I

T

S

A

R

P

S

I

C

T

T

A

K

F

W

S

N

A

V

V

M

A

V

L

K

T

S

A

V

E

E

D

E

L

L

P

P

E

L

L

R

I

I

A

N

R

E

A

A

Y

F

A

E

V

I

F

A

D

T

S

S

E

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

S

D

V

L

P

P

L
x
K

D

D

V

V

L

T

S

A

A

A

P

-

I

-

A

-

R

-

D

-

W

-

S

-

N

-

E

-

V

I

V

L

R

R

R

N

P

P

G

-

R

-

G

I

P

P

A

T

S

K

T

T

A

A

I

Q

D

D

Q

E

A

F

V

V

A

M

K

Q

-

S

-

I

-

N

-

K

-

A

-

D

-

L

L

I

N

N

A

L

A

A

K

K

R

K

P

P

M

V

I

L

I

Y

A

V

G
|
G

G
x
A

G
|
G

A

I

L

L

N
|
N

A

H

T

A

S

D

E

G

-

P

-
x
R

-

L

L

L

Q

K

E

E

V

L

S

S

T

D

R

R

L

A

A

Q

A

I

P

P

L

V

F

T

T

T

S
x
T

V
x
L

A
x
Q

G

G

K

L

G

G

L

S

L

F

P

D

P

Q

D

E

A

D

P

P

L

K

N

S

-

L

-

D

-
x
M

A
x
L

G
|
G

S
x
M

S
x
H

L

G

C

C

V

A

E

T

P

A

G

-

W

-

N

N

L

L

-

A

I

V

A

Q

E

N

A

A

D

D

V

L

V

I

L

I

A

A

V

V

G
|
G

T
x
A

E
x
R

M

F

A
x
D

D
|
D

-
x
R

-
x
V

-

T

-

G

-

N

-

I

T

S

D

K

F

F

W

A

R

P

E

E

R

A

L

R

P

R

L

A

-

A

-

E

-

G

N

R

G

G

E

G

L

I

R

H

V
x
F

D
x
E

I
x
V

D

S

P

P

R

K

K
x
N

F

I

N

N

D

K

F

V

Y

V

P

Q

C

T

A

Q

V

I

A

A

L

V

H

E

G
|
G

D
|
D

A
|
A

Q

T

T

N

L

L

S

G

G

K

L

M

E

S

R

K

L

I

P

-

T

F

D

P

V

V

R

K

E

E

A

R

S

S

A

E

A

W

I

F

A

A

S

Q

V

I

A

N

A

K

L

W

R

K

K

K

A

E

V

Y

K

P

A

Y

S

A

H

Y

-

M

-

E

G

T

P

P

L

G

Q

S

S

K

I

I

H

K

-

P

Q

Q

S

T

I

V

-

I

-

K

L

L

D

S

R

K

I

V

A

A

A

N

E

D

L

T

P

G

D

R

N

H

A

V

F

I

I

V

S

T

T

T

D

G

M
x
V

T
x
G

Q
|
Q

L
x
H

A
x
Q

Y
x
M

T

W

G

A

N

A

Y

Q

A

H

F

W

D

T

S

W

L

R

A

N

I

P

R

H

S

T

W

F

L

I

H

T

P

S

T
x
G

G
|
G

Y
x
L

G
|
G

T

T

L
x
M

G

G

Y

Y

G

G

L

L

P

P

A

A

G

A

I

I

G

G

A

A

K

Q

F

V

G

A

A

K

P

P

Q

E

R

S

P

L

G

V

L

I

V

D

L

I

V

D

G
|
G

D
|
D

G
x
A

G
x
S

F

F

L

N

Y
x
M

T

T

A

L

Q

T

E

E

L

L

A

S

T

S

S

A

V

V

E

Q

E

A

L

-

D

G

S

T

P

P

L

V

V

K

V

I

L

L

L

I

W

L

N

N

N
|
N

D

E

A
x
E

L
x
Q

G
|
G

Q
x
M

I
x
V

R

T

D

Q

-
x
W

D

Q

M

S

L

L

G

F

L

Y

D

E

I

H

E

R

P

Y

I

S

G

H

V

T

L

H

P

Q

R

L

N

N

P

P

D

D

F

F

A

I

A

K

L
|
L

A

A

R

E

A

A

F

M

G
|
G

C
x
L

T

K

V

G

T

L

Q

R

P

V

Q

K

S

K

L

Q

A

E

E

E

L

L

Q

D

T

A

D

K

L

L

R

K

H

E

G

F

F

V

K

S

R

T

N

K

G

G

V

P

T

V

L

L

I

L

E

E

L

V

K

E

active site: Y29 (≠ I27), G31 (= G29), G32 (≠ V30), A33 (≠ H31), I34 (≠ T32), E55 (= E52), T78 (≠ I75), F117 (≠ L116), Q118 (≠ H117), E119 (= E118), K167 (≠ L167), R222 (vs. gap), M258 (≠ S255), V285 (vs. gap), V401 (≠ M383), L426 (≠ Y408), G427 (= G409), M429 (≠ L411), D454 (= D436), N481 (= N464), E483 (≠ A466), Q484 (≠ L467), M486 (≠ Q469), V487 (≠ I470), W490 (vs. gap), L512 (= L494), G517 (= G499), L518 (≠ C500)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (≠ M383), G402 (≠ T384), Q403 (= Q385), H404 (≠ L386), G427 (= G409), M429 (≠ L411), G453 (= G435), D454 (= D436), A455 (≠ G437), S456 (≠ G438), M459 (≠ Y441), N481 (= N464), E483 (≠ A466), Q484 (≠ L467), G485 (= G468), M486 (≠ Q469), V487 (≠ I470)
binding ethaneperoxoic acid: G31 (= G29), G32 (≠ V30), Q118 (≠ H117)
binding flavin-adenine dinucleotide: D96 (= D93), R157 (= R157), G211 (= G214), A212 (≠ G215), G213 (= G216), N216 (= N219), T238 (≠ S238), L239 (≠ V239), Q240 (≠ A240), M255 (vs. gap), L256 (≠ A253), G257 (= G254), M258 (≠ S255), H259 (≠ S256), G278 (= G276), A279 (≠ T277), R280 (≠ E278), D282 (≠ A280), R284 (vs. gap), V285 (vs. gap), F310 (≠ V298), E311 (≠ D299), V312 (≠ I300), N316 (≠ K304), G329 (= G317), D330 (= D318), A331 (= A319), Q405 (≠ A387), M406 (≠ Y388), G424 (≠ T406), G425 (= G407)
binding magnesium ion: D454 (= D436), N481 (= N464), E483 (≠ A466)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (≠ V30), A33 (≠ H31), L35 (= L33), V107 (≠ N104), P108 (≠ H105), F117 (≠ L116), K167 (≠ L167), M258 (≠ S255), D283 (= D281), R284 (vs. gap), M486 (≠ Q469), V487 (≠ I470), W490 (vs. gap)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: Y29 (≠ I27), P30 (= P28), G31 (= G29), E55 (= E52), T78 (≠ I75), P81 (= P78), Q118 (≠ H117)

Sites not aligning to the query:

active site: 551

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
32% identity, 87% coverage: 8:470/535 of query aligns to 13:485/582 of 3ea4A

query
sites
3ea4A

G

G

G

A

Q

D

A

I

L

L

V

V

R

E

L

A

L

L

A

E

N

R

Y

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
S

L

M

E

E

L

I

Y

H

R

Q

G

A

L

L

P

T

-

R

G

S

S

S

G

S

I

I

R

R

H

N

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

S

V

F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

V

S

S

S

G

G

K

K

P

P

G

G

V

I

C

C

F

I

V

A

I
x
T

T

S

G

G

P

P

G

G

V

A

T

T

N

N

A

L

A

V

T

S

G

G

I

L

G

A

Q

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

L

V

V

A

I

I

S

T

S

G

V

Q

N

V

H

P

S

R

A

R

S

M

L

I

G

G

K

T

G

-

W

-

G

D

S

A

L
x
F

H
x
Q

E
|
E

C

T

Q

P

D

I

Q

V

R

E

A

-

M

V

T

T

A

R

P

S

I

I

T

T

A

K

F

H

S

N

A

Y

V

L

A

V

L

M

T

D

A

V

E

E

D

D

L

I

P

P

E

R

L

I

I

I

A

E

R

E

A

A

Y

F

A

F

V

L

F

A

D

T

S

S

E

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

S

D

V

V

P

P

L
x
K

D

D

V

I

L

Q

S

Q

A

Q

P

L

I

A

A

I

R

P

D

N

W

W

S

E

N

-

E

Q

V

A

V

M

R

R

R

L

P

P

G

G

R

Y

-

M

-

S

-

R

-

M

-

P

G

K

P

P

A

P

S

E

T

D

T

S

A

H

I

L

D

E

Q

Q

A

I

V

V

A

R

K

L

L

I

N

S

A

E

A

S

K

K

R

K

P

P

M

V

I

L

I

Y

A

V

G
|
G

A

C

L

L

N

N

A

S

T

S

S

D

E

E

L

L

Q

G

E

R

V

F

S

V

T

E

R

L

L

T

A

G

A

I

P

P

L

V

F

A

T

T

S
x
T

V
x
L

A
x
M

G

G

K

L

G

G

L

S

L

Y

P

P

P

C

D

D

A

D

P

E

L

L

N

S

-

L

-

H

-
x
M

A
x
L

G
|
G

S
x
M

S
x
H

L

G

C

T

V

V

E

Y

P

A

G

N

W

Y

N

A

L

-

I

V

A

E

E

H

A

S

D

D

V

L

L

L

A

A

V

F

G
|
G

T
x
V

E
x
R

M

F

A
x
D

D
|
D

T
x
R

D
x
V

F

T

W

G

R

K

-

L

E

E

R

A

L

F

P

A

L

S

N

R

G

A

E

K

L

I

L

V

R

H

V
x
I

D
|
D

I
|
I

D
|
D

P

S

R

A

K
x
E

F

I

N

G

D

K

F

N

Y

K

P

T

C

P

A

H

V

V

A

S

L

V

H

C

G
|
G

D
|
D

A
x
V

Q

K

Q

L

T

A

L

L

S

Q

G

G

L

M

L

N

E

K

R

V

L

L

P

E

T

N

D

R

V

A

R

E

E

E

A

L

S

K

A

L

A

D

I

F

A

G

S

-

V

V

A

W

A

R

L

N

R

E

K

L

A

N

V

V

K

Q

A

K

S

Q

H

K

G

F

P

P

L

L

-

S

-

F

-

K

-

T

-

F

-

G

Q

E

S

A

I

I

H

P

Q

P

S

Q

I

Y

L

A

D

I

R

K

I

V

A

L

A

D

E

E

L

L

P

T

D

D

-

G

N

K

A

A

F

I

I

I

S

S

T

T

D

G

M
x
V

T
x
G

Q
|
Q

L
x
H

A
x
Q

Y
x
M

T

W

G

A

N

A

Y

Q

A

F

F

Y

D

N

S

Y

L

K

A

K

I

P

R

R

S

Q

W

W

L

L

H

S

P

S

T
x
G

G
|
G

Y

L

G
|
G

T

A

L
x
M

G

G

Y

F

G

G

L

L

P

P

A

A

G

A

I

I

G

G

A

A

K

S

F

V

G

A

A

N

P

P

Q

D

R

A

P

I

G

V

L

V

V

D

L

I

V

D

G
|
G

D
|
D

G
|
G

G
x
S

F

F

L

I

Y

M

T

N

A

V

Q

Q

E

E

L

L

A

A

T

T

-

I

S

R

V

V

E

E

E

N

L

L

D

-

S

-

P

P

L

V

V

K

V

V

L

L

W

L

N

N

N
|
N

D

Q

A
x
H

L
|
L

G
|
G

Q
x
M

I
x
V

active site: Y32 (≠ I27), G34 (= G29), G35 (≠ V30), A36 (≠ H31), S37 (≠ T32), E58 (= E52), T81 (≠ I75), F120 (≠ L116), Q121 (≠ H117), E122 (= E118), K170 (≠ L167), M265 (≠ S255), V292 (≠ D283), V399 (≠ M383), G425 (= G409), M427 (≠ L411), D452 (= D436), N479 (= N464), H481 (≠ A466), L482 (= L467), M484 (≠ Q469), V485 (≠ I470)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D281), R291 (≠ T282), M484 (≠ Q469), V485 (≠ I470)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R157), G221 (= G214), G222 (= G215), G223 (= G216), T245 (≠ S238), L246 (≠ V239), M247 (≠ A240), M262 (vs. gap), L263 (≠ A253), G264 (= G254), M265 (≠ S255), H266 (≠ S256), G285 (= G276), V286 (≠ T277), R287 (≠ E278), D289 (≠ A280), R291 (≠ T282), V292 (≠ D283), I308 (≠ V298), D309 (= D299), I310 (= I300), D311 (= D301), E314 (≠ K304), G327 (= G317), D328 (= D318), V329 (≠ A319), Q403 (≠ A387), M404 (≠ Y388), G422 (≠ T406), G423 (= G407)
binding magnesium ion: D452 (= D436), N479 (= N464), H481 (≠ A466)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ M383), G400 (≠ T384), Q401 (= Q385), H402 (≠ L386), G425 (= G409), M427 (≠ L411), G451 (= G435), D452 (= D436), G453 (= G437), S454 (≠ G438), N479 (= N464), H481 (≠ A466), L482 (= L467), G483 (= G468), M484 (≠ Q469), V485 (≠ I470)

Sites not aligning to the query:

active site: 488, 557
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 488, 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
32% identity, 87% coverage: 8:470/535 of query aligns to 13:485/582 of 3e9yA

query
sites
3e9yA

G

G

G

A

Q

D

A

I

L

L

V

V

R

E

L

A

L

L

A

E

N

R

Y

Q

G

G

V

V

D

E

T

T

V

V

F

F

G

A

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
S

L

M

E

E

L

I

Y

H

R

Q

G

A

L

L

P

T

-

R

G

S

S

S

G

S

I

I

R

R

H

N

V

V

L

L

T

P

R

R

H

H

E
|
E

Q

Q

G

G

A

G

S

V

F

F

M

A

A

A

D

E

G

G

Y

Y

A

A

R

R

V

S

S

S

G

G

K

K

P

P

G

G

V

I

C

C

F

I

V

A

I
x
T

T

S

G

G

P

P

G

G

V

A

T

T

N

N

A

L

A

V

T

S

G

G

I

L

G

A

Q

D

A

A

Y

L

A

L

D

D

S

S

I

V

P

P

M

L

L

V

V

A

I

I

S

T

S

G

V

Q

N

V

H

P

S

R

A

R

S

M

L

I

G

G

K

T

G

-

W

-

G

D

S

A

L
x
F

H
x
Q

E
|
E

C

T

Q

P

D

I

Q

V

R

E

A

-

M

V

T

T

A

R

P

S

I

I

T

T

A

K

F

H

S

N

A

Y

V

L

A

V

L

M

T

D

A

V

E

E

D

D

L

I

P

P

E

R

L

I

I

I

A

E

R

E

A

A

Y

F

A

F

V

L

F

A

D

T

S

S

E

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

S

D

V

V

P

P

L
x
K

D

D

V

I

L

Q

S

Q

A

Q

P

L

I

A

A

I

R

P

D

N

W

W

S

E

N

-

E

Q

V

A

V

M

R

R

R

L

P

P

G

G

R

Y

-

M

-

S

-

R

-

M

-

P

G

K

P

P

A

P

S

E

T

D

T

S

A

H

I

L

D

E

Q

Q

A

I

V

V

A

R

K

L

L

I

N

S

A

E

A

S

K

K

R

K

P

P

M

V

I

L

I

Y

A

V

G
|
G

A

C

L

L

N

N

A

S

T

S

S

D

E

E

L

L

Q

G

E

R

V

F

S

V

T

E

R

L

L

T

A

G

A

I

P

P

L

V

F

A

T

T

S
x
T

V
x
L

A
x
M

G

G

K

L

G

G

L

S

L

Y

P

P

P

C

D

D

A

D

P

E

L

L

N

S

-

L

-

H

-
x
M

A
x
L

G
|
G

S
x
M

S
x
H

L

G

C

T

V

V

E

Y

P

A

G

N

W

Y

N

A

L

-

I

V

A

E

E

H

A

S

D

D

V

L

L

L

A

A

V

F

G
|
G

T
x
V

E
x
R

M

F

A
x
D

D
|
D

T
x
R

D
x
V

F

T

W

G

R

K

-

L

E

E

R

A

L

F

P

A

L

S

N

R

G

A

E

K

L

I

L

V

R

H

V
x
I

D
|
D

I
|
I

D
|
D

P

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R

A

K
x
E

F

I

N

G

D

K

F

N

Y

K

P

T

C

P

A

H

V

V

A

S

L

V

H

C

G
|
G

D
|
D

A
x
V

Q

K

Q

L

T

A

L

L

S

Q

G

G

L

M

L

N

E

K

R

V

L

L

P

E

T

N

D

R

V

A

R

E

E

E

A

L

S

K

A

L

A

D

I

F

A

G

S

-

V

V

A

W

A

R

L

N

R

E

K

L

A

N

V

V

K

Q

A

K

S

Q

H

K

G

F

P

P

L

L

-

S

-

F

-

K

-

T

-

F

-

G

Q

E

S

A

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H

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Q

I

Y

L

A

D

I

R

K

I

V

A

L

A

D

E

E

L

L

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D

D

-

G

N

K

A

A

F

I

I

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S

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G

M
x
V

T
x
G

Q
|
Q

L
x
H

A
x
Q

Y
x
M

T

W

G

A

N

A

Y

Q

A

F

F

Y

D

N

S

Y

L

K

A

K

I

P

R

R

S

Q

W

W

L

L

H

S

P

S

T
x
G

G
|
G

Y

L

G
|
G

T

A

L
x
M

G

G

Y

F

G

G

L

L

P

P

A

A

G

A

I

I

G

G

A

A

K

S

F

V

G

A

A

N

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P

Q

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A

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I

G

V

L

V

V

D

L

I

V

D

G
|
G

D
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D

G
|
G

G
x
S

F

F

L

I

Y
x
M

T

N

A

V

Q

Q

E

E

L

L

A

A

T

T

-

I

S

R

V

V

E

E

E

N

L

L

D

-

S

-

P

P

L

V

V

K

V

V

L

L

W

L

N

N

N
|
N

D

Q

A
x
H

L
|
L

G
|
G

Q
x
M

I
x
V

active site: Y32 (≠ I27), G34 (= G29), G35 (≠ V30), A36 (≠ H31), S37 (≠ T32), E58 (= E52), T81 (≠ I75), F120 (≠ L116), Q121 (≠ H117), E122 (= E118), K170 (≠ L167), M265 (≠ S255), V292 (≠ D283), V399 (≠ M383), G425 (= G409), M427 (≠ L411), D452 (= D436), N479 (= N464), H481 (≠ A466), L482 (= L467), M484 (≠ Q469), V485 (≠ I470)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D281), R291 (≠ T282), M484 (≠ Q469)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R157), G221 (= G214), G222 (= G215), G223 (= G216), T245 (≠ S238), L246 (≠ V239), M247 (≠ A240), M262 (vs. gap), L263 (≠ A253), G264 (= G254), M265 (≠ S255), H266 (≠ S256), G285 (= G276), V286 (≠ T277), R287 (≠ E278), D289 (≠ A280), R291 (≠ T282), V292 (≠ D283), I308 (≠ V298), D309 (= D299), I310 (= I300), D311 (= D301), E314 (≠ K304), G327 (= G317), D328 (= D318), V329 (≠ A319), Q403 (≠ A387), M404 (≠ Y388), G422 (≠ T406), G423 (= G407)
binding magnesium ion: D452 (= D436), N479 (= N464), H481 (≠ A466)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ M383), G400 (≠ T384), Q401 (= Q385), H402 (≠ L386), G425 (= G409), M427 (≠ L411), G451 (= G435), D452 (= D436), G453 (= G437), S454 (≠ G438), M457 (≠ Y441), N479 (= N464), H481 (≠ A466), L482 (= L467), G483 (= G468), M484 (≠ Q469), V485 (≠ I470)

Sites not aligning to the query:

active site: 488, 557
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 488, 567

1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
29% identity, 98% coverage: 6:530/535 of query aligns to 8:552/595 of 1t9bB

query
sites
1t9bB

L

L

T

T

G

G

Q

Q

A

I

L

F

V

N

R

E

L

M

A

S

N

R

Y

Q

G

N

V

V

D

D

T

T

V

V

F

F

G

G

I
x
Y

P

P

G
|
G

V
x
G

H
x
A

T
x
I

L

L

E

P

L

V

Y

Y

R

D

G

A

L

I

P

H

G

N

S

S

-

D

G

K

I

F

R

N

H

F

V

V

L

L

T

P

R

K

H

H

E
|
E

Q

Q

G

G

A

A

S

G

F

H

M

M

A

A

D

E

G

G

Y

Y

A

A

R

R

V

A

S

S

G

G

K

K

P

P

G

G

V

V

C

V

F

L

V

V

I
x
T

T

S

G

G

P

P

G

G

V

A

T

T

N

N

A

V

T

T

G

P

I

M

G

A

Q

D

A

A

Y

F

A

A

D
|
D

S

G

I

I

P

P

M

M

L

V

V

V

I

F

S

T

S

G

V

Q

N
x
V

H
x
P

S

T

A

S

S

A

L

I

G

G

K

T

G

-

W

-

G

D

S

A

L
x
F

H
x
Q

E
|
E

C

A

Q

-

D

D

Q

V

R

V

A

G

M

I

T

S

A

R

P

S

I

C

T

T

A

K

F

W

S

N

A

V

V

M

A

V

L

K

T

S

A

V

E

E

D

E

L

L

P

P

E

L

L

R

I

I

A

N

R

E

A

A

Y

F

A

E

V

I

F

A

D

T

S

S

E

G

R
|
R

P

P

R

G

P

P

V

V

H

L

I

V

S

D

V

L

P

P

L
x
K

D

D

V

V

L

-

S

T

A

A

P

A

I

I

A

L

R

R

D

N

W

P

S

I

N

P

E

T

V

K

V

T

R

T

R

L

P

P

G

S

R

R

G

A

P

Q

A

D

S

E

-

F

-

V

T

M

T

Q

A

S

I

I

D

N

Q

K

A

A

V

A

A

D

K

L

L

I

N

N

A

L

A

A

K

K

R

K

P

P

M

V

I

L

I

Y

A

V

G
|
G

G
x
A

G
|
G

A

I

L

L

N
|
N

A

H

T

A

S

D

E

G

-

P

-
x
R

-

L

L

L

Q

K

E

E

V

L

S

S

T

D

R

R

L

A

A

Q

A

I

P

P

L

V

F

T

T

T

S
x
T

V
x
L

A
x
Q

G

G

K

L

G

G

L

S

L

F

P

D

P

Q

D

E

A

D

P

P

L

K

N

S

-

L

-

D

-
x
M

A
x
L

G
|
G

S
x
M

S
x
H

L

G

C

C

V

A

E

T

P

A

G

-

W

-

N

N

L

L

-

A

I

V

A

Q

E

N

A

A

D

D

V

L

V

I

L

I

A

A

V

V

G
|
G

T
x
A

E
x
R

M

F

A
x
D

D
|
D

-
x
R

-
x
V

-

T

-

G

-

N

-

I

T

S

D

K

F

F

W

A

R

P

E

E

R

A

L

R

P

R

L

A

-

A

-

E

-

G

N

R

G

G

E

G

L

I

R

H

V
x
F

D
x
E

I
x
V

D

S

P

P

R

K

K
x
N

F

I

N

N

D

K

F

V

Y

V

P

Q

C

T

A

Q

V

I

A

A

L

V

H

E

G
|
G

D
|
D

A
|
A

Q

T

T

N

L

L

S

G

G

K

L

M

E

S

R

K

L

I

P

-

T

F

D

P

V

V

R

K

E

E

A

R

S

S

A

E

A

W

I

F

A

A

S

Q

V

I

A

N

A

K

L

W

R

K

K

K

A

E

V

Y

K

P

A

Y

S

A

H

Y

-

M

-

E

G

T

P

P

L

G

Q

S

S

K

I

I

H

K

-

P

Q

Q

S

T

I

V

-

I

-

K

L

L

D

S

R

K

I

V

A

A

A

N

E

D

L

T

P

G

D

R

N

H

A

V

F

I

I

V

S

T

T

T

D

G

M
x
V

T

G

Q

Q

L

H

A
x
Q

Y
x
M

T

W

G

A

N

A

Y

Q

A

H

F

W

D

T

S

W

L

R

A

N

I

P

R

H

S

T

W

F

L

I

H

T

P

S

T
x
G

G
|
G

Y
x
L

G
|
G

T

T

L
x
M

G

G

Y

Y

G

G

L

L

P

P

A

A

G

A

I

I

G

G

A

A

K

Q

F

V

G

A

A

K

P

P

Q

E

R

S

P

L

G

V

L

I

V

D

L

I

V

D

G

G

D
|
D

G

A

G

S

F

F

L

N

Y

M

T

T

A

L

Q

T

E

E

L

L

A

S

T

S

S

A

V

V

E

Q

E

A

L

-

D

G