SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_3221 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3221 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
35% identity, 95% coverage: 20:449/452 of query aligns to 40:469/472 of P78061

query
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P78061

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H282 (= H261) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R337) mutation to Q: Activity is impaired to 3% of wild-type.

4s0rD Structure of gs-tnra complex (see paper)
30% identity, 82% coverage: 78:449/452 of query aligns to 70:444/447 of 4s0rD

query
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4s0rD

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active site: E135 (= E140), E137 (= E142), E192 (= E205), E199 (= E212), H248 (= H261), R319 (= R332), E336 (= E349), R338 (= R351)
binding glutamine: E137 (= E142), E192 (= E205), R301 (= R314), E307 (≠ S320)
binding magnesium ion: E135 (= E140), E135 (= E140), E199 (= E212), H248 (= H261), H248 (= H261), E336 (= E349), H419 (≠ G424)
binding : D161 (≠ S174), G241 (≠ D254), V242 (≠ E255), N243 (≠ P256), G305 (≠ D318), Y306 (≠ T319), Y376 (= Y389), I426 (≠ A431), M430 (≠ N435)

Sites not aligning to the query:

active site: 56
binding magnesium ion: 66
binding : 63, 64, 65, 66

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
30% identity, 82% coverage: 78:449/452 of query aligns to 67:441/444 of P12425

query
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P12425

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E132 (= E140) binding
E134 (= E142) binding
E189 (= E205) binding
V190 (≠ D206) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E212) binding
G241 (= G257) binding
H245 (= H261) binding
G302 (≠ D318) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ S320) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P322) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E349) binding
E424 (= E432) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
59 G→R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
62 Important for inhibition by glutamine; R→A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
30% identity, 82% coverage: 78:449/452 of query aligns to 66:440/443 of 4lnkA

query
sites
4lnkA

D

D

M

M

V

Y

C

L

K

Y

P

P

D

D

A

L

D

N

A

T

V

F

Y

V

V

I

V

F

P

P

W

W

A

T

I

A

E

E

P

K

T

G

A

K

I

V

V

A

-

R

-

F

I

I

H

C

D

D

T

I

F

Y

D

N

K

P

F

D

G

G

N

T

P

P

I

F

E

E

L

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

K

R

V

I

L

L

Q

K

L

E

Y

M

T

E

D

D

K

L

G

G

W

F

R

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

R

L

C

D

E

E

-

K

-

G

D

E

P

P

D

T

L

L

P

E

L

L

K

N

A

D

P

K

M

G

G

G

R

Y

S

-

G

-

R

-

A

-

E

-

S

-

G

-

R

-

Q

-

S

-

F

F

S

D

I

L

D

A

A

P

A

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

W

E

C

L

E

E

L

E

Q

M

G

G

L

F

D

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

D
x
V

G

A

P

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F
|
F

R

K

H
x
Y

G

A

N

G

A

A

L

V

D

R

L

S

A

C

D

D

Q

D

I

I

T

Q

V

T

F

F

K

K

R

L

T

V

M

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

N

G

V

L

A

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

V

L

G

F

D

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

L

C

H
x
N

Q

L

S
|
S

V

L

V

F

D

K

I

N

A

G

T

V

G

N

Q

A

P

F

I

F

F

D

A

E

D

N

A

A

D

D

G

L

N

Q

M

L

S

S

E

E

L

T

F

A

L

K

H

H

H

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

I

A

P

T

K

S

V

F

L

T

P

A

M

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R

R

F

L

L

V

P

P

D

G

T

Y

S
x
E

A

A

P

P

V

C

N

Y

V

V

E

A

W

W

G

S

E

A

E

Q

N

N

R
|
R

T

S

V

P

G

L

L

I

R

R

V

I

P

P

T

A

S
|
S

S

R

P

G

D

I

A
x
S

M

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

A

V

S

L

L

L

L

A

C

A

G

G

Y

L

L

D

G

G

M

I

V

K

E

N

R

K

I

L

E

E

P

A

S

P

A

A

A

P

V

I

Q

D

G

R

R

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

-

M

E

E

R

N

R

G

N

I

L

V

R

D

L

L

P

P

I

A

T

T

I

L

E

A

D

E

A

A

L

L

T

E

Q

E

M

F

E

K

E

S

C

N

V

E

T

V

I

M

G

V

R

K

Y

A

L

L

G

G

S

E

K

H

F

L

V

F

R

E

G

H

Y

F

V

I

A

E

V

A

K

K

R

E

A

I

E

E

H

W

E

D

N

M

F

F

K

R

R

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

E

E

F

Q

L

Y

M

M

active site: E131 (= E140), E133 (= E142), E188 (= E205), E195 (= E212), H244 (= H261), R315 (= R332), E332 (= E349), R334 (= R351)
binding adenosine-5'-diphosphate: F198 (= F215), Y200 (≠ H217), N246 (≠ H263), S248 (= S265), S324 (= S341), S328 (≠ A345), R330 (= R347)
binding glutamic acid: E133 (= E142), E188 (= E205), V189 (≠ D206), N239 (≠ P256), G240 (= G257), G242 (≠ A259), E303 (≠ S320)
binding magnesium ion: E131 (= E140), E188 (= E205), E195 (= E212), H244 (= H261), E332 (= E349)

Sites not aligning to the query:

active site: 52
binding adenosine-5'-diphosphate: 43, 50

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
30% identity, 82% coverage: 78:449/452 of query aligns to 66:440/443 of 4lniA

query
sites
4lniA

D

D

M

M

V

Y

C

L

K

Y

P

P

D

D

A

L

D

N

A

T

V

F

Y

V

V

I

V

F

P

P

W

W

A

T

I

A

E

E

P

K

T

G

A

K

I

V

V

A

-

R

-

F

I

I

H

C

D

D

T

I

F

Y

D

N

K

P

F

D

G

G

N

T

P

P

I

F

E

E

L

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

K

R

V

I

L

L

Q

K

L

E

Y

M

T

E

D

D

K

L

G

G

W

F

R

S

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

R

L

C

D

E

E

-

K

-

G

D

E

P

P

D

T

L

L

P

E

L

L

K

N

A

D

P

K

M

G

G

G

R

Y

S

-

G

-

R

-

A

-

E

-

S

-

G

-

R

-

Q

-

S

-

F

F

S

D

I

L

D

A

A

P

A

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

W

E

C

L

E

E

L

E

Q

M

G

G

L

F

D

E

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

D

V

G

A

P

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

R

K

H
x
Y

G

A

N

G

A

A

L

V

D

R

L

S

A

C

D

D

Q

D

I

I

T

Q

V

T

F

F

K

K

R

L

T

V

M

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

N

G

V

L

A

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

V

L

G

F

D

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

L

C

H
x
N

Q

L

S
|
S

V

L

V

F

D

K

I

N

A

G

T

V

G

N

Q

A

P

F

I

F

F

D

A

E

D

N

A

A

D

D

G

L

N

Q

M

L

S

S

E

E

L

T

F

A

L

K

H

H

H

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

I

A

P

T

K

S

V

F

L

T

P

A

M

V

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

F

L

L

V

P

P

D

G

T

Y

S
x
E

A

A

P

P

V

C

N

Y

V

V

E

A

W

W

G

S

E

A

E

Q

N

N

R
|
R

T

S

V

P

G

L

L

I

R
|
R

V

I

P

P

T

A

S

S

S

R

P

G

D

I

A

S

M

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

A

V

S

L

L

L

L

A

C

A

G

G

Y

L

L

D

G

G

M

I

V

K

E

N

R

K

I

L

E

E

P

A

S

P

A

A

A

P

V

I

Q

D

G

R

R

N

A

I

Y

Y

-

V

-

M

-

S

-

K

-

E

-

R

-

M

E

E

R

N

R

G

N

I

L

V

R

D

L

L

P

P

I

A

T

T

I

L

E

A

D

E

A

A

L

L

T

E

Q

E

M

F

E

K

E

S

C

N

V

E

T

V

I

M

G

V

R

K

Y

A

L

L

G

G

S

E

K

H

F

L

V

F

R

E

G

H

Y

F

V

I

A

E

V

A

K

K

R

E

A

I

E

E

H

W

E

D

N

M

F

F

K

R

R

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

E

E

F

Q

L

Y

M

M

active site: E131 (= E140), E133 (= E142), E188 (= E205), E195 (= E212), H244 (= H261), R315 (= R332), E332 (= E349), R334 (= R351)
binding adenosine-5'-diphosphate: E131 (= E140), E183 (≠ D200), D197 (≠ N214), Y200 (≠ H217), N246 (≠ H263), S248 (= S265), R320 (= R337), R330 (= R347)
binding magnesium ion: E131 (= E140), E131 (= E140), E133 (= E142), E188 (= E205), E195 (= E212), E195 (= E212), H244 (= H261), E332 (= E349)
binding l-methionine-s-sulfoximine phosphate: E133 (= E142), E188 (= E205), H244 (= H261), R297 (= R314), E303 (≠ S320), R315 (= R332), R334 (= R351)

Sites not aligning to the query:

active site: 52

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
29% identity, 82% coverage: 78:446/452 of query aligns to 65:433/439 of 7tdpA

query
sites
7tdpA

D

D

M

M

V

Y

C

L

K

Y

P

P

D

D

A

L

D

D

A

T

V

W

Y

V

V

V

V

F

P

P

W

W

A

S

I

-

E

D

P

R

T

V

A

A

I

R

V

L

I

I

H

C

D

D

T

I

F

Y

D

K

K

P

F

D

G

G

N

S

P

P

I

F

E

A

L

G

S

D

P

P

R

R

N

G

V

I

L

L

K

K

K

R

V

V

L

L

Q

K

L

E

Y

A

T

E

D

E

K

L

G

G

W

Y

R

T

P

S

I

M

-
x
N

V

V

A
x
G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

R

T

C

D

E

E

D

K

P

G

D

D

L

-

P

P

L

T

K

T

A

E

P

L

M

N

G

D

R

Q

S

G

G

G

R

Y

A

F

E

D

S

L

G

A

R

P

Q

M

S

D

F

L

S

G

I

E

D

N

A

C

A

R

N

R

E

E

F

I

D

V

P

L

L

K

F

L

E

E

D

E

V

M

Y

-

D

-

W

-

C

-

E

-

L

-

Q

-

G

G

L

F

D

E

L

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

D

V

G

A

P

P

A

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

R

K

H
x
Y

G

A

N

D

A

A

L

V

D

K

L

A

A

A

D

D

Q

Q

I

I

T

Q

V

T

F

F

K

K

R

L

T

V

M

V

R

K

E

T

A

I

A

A

L

R

K

Q

H

H

N

G

V

L

A

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

V

L

G

F

D

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

L

C

H
x
N

Q

Q

S
|
S

V

L

V

-

D

-

I

F

A

K

T

D

G

N

Q

E

P

N

I

V

F

F

A

Y

D

D

A

E

-

T

-

D

D

E

G

L

N

G

M

L

S

S

E

Q

L

T

F

A

L

R

H

H

H

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

I

A

P

R

K

A

V

M

L

A

P

A

M

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

F

L

L

V

P

P

D

G

T

Y

S
x
E

A

A

P

P

V

C

N

Y

V

V

E

A

W

W

G

S

E

A

E

S

N

N

R
|
R

T

S

V

P

G

M

L

I

R
|
R

V

I

P

P

T

A

S

S

S

R

P

G

D

L

A

S

M

T

R
|
R

V

V

E
|
E

N

V

R
|
R

L

N

P

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

A

V

S

M

L

L

L

R

C

A

G

G

Y

L

L

D

G

G

M

I

V

K

E

R

R

Q

I

M

E

A

P

L

S

P

A

A

A

P

V

I

Q

D

G

R

R

N

A

I

Y

Y

-

V

-

M

-

S

-

E

-

R

-

I

E

E

R

E

R

G

N

I

L

P

R

S

L

L

P

P

I

A

T

D

I

L

E

K

D

E

A

A

L

L

T

S

Q

E

M

L

E

I

E

R

C

S

V

E

T

V

I

I

G

S

R

D

Y

A

L

L

G

G

S

D

K

H

F

A

V

L

R

A

G

Y

Y

F

V

Y

A

E

V

L

K

K

R

E

A

I

E

E

H

W

E

D

N

M

F

Y

K

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

E

D

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A138), E127 (= E140), E179 (≠ D200), D193 (≠ N214), Y196 (≠ H217), N242 (≠ H263), S244 (= S265), R316 (= R337), R326 (= R347)
binding magnesium ion: E127 (= E140), E127 (= E140), E129 (= E142), E184 (= E205), E191 (= E212), E191 (= E212), H240 (= H261), E328 (= E349)
binding l-methionine-s-sulfoximine phosphate: E127 (= E140), E129 (= E142), E184 (= E205), E191 (= E212), G236 (= G257), H240 (= H261), R293 (= R314), E299 (≠ S320), R311 (= R332), R330 (= R351)

7tf6A Glutamine synthetase (see paper)
30% identity, 82% coverage: 78:449/452 of query aligns to 65:435/438 of 7tf6A

query
sites
7tf6A

D

D

M

M

V

Y

C

L

K

H

P

P

D

D

A

L

D

D

A

T

V

W

Y

V

V

I

V

F

P

P

W

W

A

G

I

-

E

-

P

K

T

V

A

A

I

R

V

L

I

I

H

C

D

D

T

V

F

Y

D

K

K

T

F

D

G

G

N

T

P

P

I

F

E

E

L

G

S

D

P

P

R

R

N

A

V

N

L

L

K

K

K

R

V

V

L

L

Q

K

L

E

Y

M

T

E

D

D

K

L

G

G

W

F

R

T

P

D

I

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

R

L

C

D

E

E

-

K

-

G

D

E

P

P

D

T

L

L

P

E

L

L

K

N

A

D

P

D

M

G

G

G

R

-

S

-

G

-

R

-

A

-

E

-

S

-

G

-

R

-

Q

-

S

Y

F

F

S

D

I

L

D

A

A

P

A

T

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

W

E

C

L

E

E

L

D

Q

M

G

G

L

F

D

D

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

D

V

G

A

P

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

R

K

H

Y

G

A

N

D

A

A

L

V

D

T

L

A

A

C

D

D

Q

N

I

I

T

Q

V

T

F

F

K

K

R

L

T

V

M

V

R

K

E

T

A

I

A

A

L

R

K

K

H

H

N

N

V

L

A

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

V

L

G

F

D
x
G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

L

F

H

N

Q

V

S

S

V

L

V

F

D

K

I

G

A

K

T

E

G

N

Q

A

P

F

I

F

F

D

A

P

D

N

A

T

D

E

G

M

N

G

M

L

S

T

E

E

L

T

F

A

L

Y

H

Q

H

F

I

T

G

A

G

G

L

V

Q

L

K

K

Y

N

I

A

P

R

K

G

V

F

L

T

P

A

M

V

F

C

A

N

P

P

N

L

V

V

N

N

S

S

F

Y

R

K

R
|
R

F

L

L

V

P

P

D
x
G

T
x
Y

S
x
E

A

A

P

P

V

C

N

Y

V

I

E

A

W

W

G

S

E

G

E

K

N

N

R
|
R

T

S

V

P

G

L

L

I

R

R

V

V

P

P

T

S

S

S

S

R

P

G

D

L

A

S

M

T

R

R

V

I

E
|
E

N

V

R

R

L

S

P

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

I

L

A

A

S

I

L

L

L

E

C

A

G

G

Y

L

L

D

G

G

M

I

V

K

E

N

R

K

I

L

E

K

P

V

S

P

A

E

A

P

V

V

Q

N

G

Q

R

N

A

I

Y
|
Y

E

E

-

M

R

N

R

R

N

E

L

E

R

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

I

S

T

T

I

L

E

Y

D

T

A

A

L

L

T

K

Q

A

M

M

E

R

E

E

C

N

V

E

T

V

I

I

G

K

R

K

Y

A

L

L

G

G

S

N

K

H

F

I

V

Y

R

N

G

Q

Y

F

V

I

A

N

V

S

K

K

R

S

A

I

E

E

H

W

E

D

N
x
Y

F

Y

K

R

R

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

E

D

F
x
Q

L

Y

M

M

binding glutamine: E128 (= E142), E183 (= E205), G235 (= G257), H239 (= H261), R292 (= R314), E298 (≠ S320)
binding magnesium ion: E126 (= E140), E128 (= E142), E183 (= E205), E190 (= E212), H239 (= H261), E327 (= E349)
binding : G232 (≠ D254), N234 (≠ P256), G296 (≠ D318), Y297 (≠ T319), R310 (= R332), Y367 (= Y389), Y421 (≠ N435), Q433 (≠ F447)

Sites not aligning to the query:

binding : 58, 60, 437

7tfaB Glutamine synthetase (see paper)
30% identity, 83% coverage: 78:450/452 of query aligns to 65:440/441 of 7tfaB

query
sites
7tfaB

D

D

M

M

V

Y

C

L

K

Y

P

P

D

D

A

L

D

D

A

T

V

W

Y

V

V

V

V

F

P

P

W

W

A

V

I

T

-

S

E

D

P

R

T

V

A

A

I

R

V

L

I

I

H

C

D

D

T

I

F

Y

D

K

K

P

F

D

G

G

N

S

P

P

I

F

E

A

L

G

S

D

P

P

R

R

N

G

V

I

L

L

K

K

K

R

V

V

L

L

Q

K

L

E

Y

A

T

E

D

E

K

L

G

G

W

Y

R

T

P

S

I

M

-

N

V

V

A

G

P

P

E
|
E

M

P

E
|
E

F

F

Y

F

L

L

T

F

Q

K

R

T

C

D

E

E

D

K

P

G

D

D

L

-

P

P

L

T

K

T

A

E

P

L

M

N

G

D

R

Q

S

G

G

G

R
x
Y

A

F

E

D

S

L

G

A

R

P

Q

M

S

D

F

L

S

G

I

E

D

N

A

C

A

R

N

R

E

E

F

I

D

V

P

L

L

K

F

L

E

E

D

E

V

M

Y

-

D

-

W

-

C

-

E

-

L

-

Q

-

G

G

L

F

D

E

L

I

D

E

T

A

L

S

I

H

H

H

E
|
E

D
x
V

G

A

P

P

A

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

R

K

H

Y

G

A

N

D

A

A

L

V

D

K

L

A

A

A

D

D

Q

Q

I

I

T

Q

V

T

F

F

K

K

R

L

T

V

M

V

R

K

E

T

A

I

A

A

L

R

K

Q

H

H

N

G

V

L

A

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

V

L

G

F

D

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

L

C

H

N

Q

Q

S

S

V

L

V

-

D

-

I

F

A

K

T

D

G

N

Q

E

P

N

I

V

F

F

A

Y

D

D

A

E

-

T

-

D

D

E

G

L

N

G

M

L

S

S

E

Q

L

T

F

A

L

R

H

H

H

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

I

A

P

R

K

A

V

M

L

A

P

A

M

I

F

T

A

N

P

P

N

T

V

V

N

N

S

S

F

Y

R

K

R
|
R

F

L

L

V

P

P

D
x
G

T
x
Y

S
x
E

A

A

P

P

V

C

N

Y

V

V

E

A

W

W

G

S

E

A

E

S

N

N

R
|
R

T

S

V

P

G

M

L

I

R

R

V

I

P

P

T

A

S

S

S

R

P

G

D

L

A

S

M

T

R

R

V

V

E
|
E

N

V

R

R

L

N

P

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

A

V

S

M

L

L

L

R

C

A

G

G

Y

L

L

D

G

G

M

I

V

K

E

R

R

Q

I

M

E

A

P

L

S

P

A

A

A

P

V

I

Q

D

G

R

R

N

A

I

Y

Y

-

V

-

M

-

S

-

E

-

R

-

I

E

E

R

E

R

G

N

I

L

P

R

S

L

L

P

P

I

A

T

D

I

L

E

K

D

E

A

A

L

L

T

S

Q

E

M

L

E

I

E

R

C

S

V

E

T

V

I

I

G

S

R

D

Y

A

L

L

G

G

S

D

K

H

F

A

V

L

R

A

G

Y

Y

F

V

Y

A

E

V

L

K

K

R

E

A

I

E

E

H

W

E

D

N
x
M

F

Y

K

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

-

D

E

Q

F

Y

L

L

M

T

L

L

binding glutamine: E131 (= E142), Y153 (≠ R165), E186 (= E205), G238 (= G257), H242 (= H261), R295 (= R314), E301 (≠ S320)
binding magnesium ion: E129 (= E140), E131 (= E142), E186 (= E205), E193 (= E212), H242 (= H261), E330 (= E349)
binding : V187 (≠ D206), N237 (≠ P256), G299 (≠ D318), Y300 (≠ T319), R313 (= R332), M424 (≠ N435)

Sites not aligning to the query:

binding : 58, 60

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
31% identity, 81% coverage: 80:444/452 of query aligns to 55:390/396 of 5dm3C

query
sites
5dm3C

V

I

C

M

K

K

P

P

D

D

A

L

D

A

A

T

V

L

Y

R

V

C

V

V

P

P

W

W

A

-

I

L

E

E

P

G

T

T

A

A

I

M

V

V

I

L

H

C

D

D

T

L

F

L

D

D

-

H

K

R

F

T

G

H

N

A

P

E

I

V

E

P

L

H

S

A

P

P

R

R

N

A

V

I

L

L

K

K

K

R

V

Q

L

L

Q

A

L

R

Y

L

T

E

D

A

K

M

G

G

W

L

R

E

P

A

I

I

V

M

A

A

P

T

E
|
E

M

L

E
|
E

F

F

Y

F

L

L

T

F

Q

E

R

K

C

S

E

L

D

D

P

E

D

I

L

-

P

-

L

-

K

-

A

-

P

-

M

-

G

-

R

R

S

K

G

G

R

R

A

F

E

R

S

T

G

T

R

K

Q

E

S

E

F

H

S

V

I

L

D

R

A

-

N

-

E

-

F

-

D

-

P

P

L

L

F

R

E

N

D

H

V

L

Y

H

D

-

W

-

C

-

E

-

L

A

Q

A

G

G

L

I

D

P

L

V

D

E

T

G

L

T

I

K

H

G

E
|
E

D

A

G

G

P

A

A

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

R
|
R

H
x
C

G

A

N

K

A

A

L

L

D

D

L

T

A

A

D

D

Q

Y

I

H

T

T

V

I

F

A

K

K

R

H

T

A

M

T

R

K

E

E

A

I

A

A

L

W

K

Q

H

Q

N

G

V

R

A

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

V

H

G

H

D

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

L

I

H
|
H

Q

Q

S
|
S

V

L

V

W

D

K

I

-

A

-

T

Q

G

G

Q

L

P

P

I

A

F

F

A

H

D

D

A

E

D

R

G

D

-

A

-

L

N

G

M

M

S

S

E

A

L

L

F

M

L

K

H

H

H

Y

I

L

G

A

G

G

L

L

Q

L

K

K

Y

Y

I

A

P

P

K

D

V

Y

L

T

P

Y

M

F

F

L

A

A

P

P

N

Y

V

L

N

N

S

S

F

Y

R

K

R

R

F

F

L

-

P

-

D

-

T

Q

S

F

A

A

P

P

V

T

N

R

V

T

E

V

W

W

G

S

E

V

E

D

N

N

R
|
R

T

T

V

A

G

G

L

F

R

R

V

L

P

C

T

A

S

E

S

G

P

T

D

R

A

A

M

V

R
|
R

V

I

E
|
E

N

C

R
|
R

L

I

P

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

A

G

S

Q

L

L

L

A

C

A

G

G

Y

I

L

K

G

G

M

I

V

E

E

E

R

C

I

L

E

A

P

L

S

P

A

P

V

A

Q

S

G

G

R

-

A

-

Y

-

E

-

R

-

N

-

L

-

R

L

L

I

P

P

I

Q

T

N

I

L

E

R

D

D

A

A

L

M

T

E

Q

A

M

L

E

R

E

G

C

S

V

T

T

M

I

L

G

R

R

E

Y

A

L

M

G

G

S

E

K

D

F

V

V

V

R

D

G

H

Y

Y

V

V

A

R

V

A

K

A

R

E

A

V

E

E

H

L

E

E

N

D

F

F

K

Q

R

R

V

V

I

V

S

S

S

D

W

Y

E

E

active site: E115 (= E140), E117 (= E142), E162 (= E205), E169 (= E212), H218 (= H261), R286 (= R332), E303 (= E349), R305 (= R351)
binding adenosine-5'-diphosphate: R173 (= R216), C174 (≠ H217), H220 (= H263), S222 (= S265), R301 (= R347)

7tdvC Glutamine synthetase (see paper)
30% identity, 82% coverage: 78:449/452 of query aligns to 66:440/443 of 7tdvC

query
sites
7tdvC

D

D

M

M

V

Y

C

L

K

H

P

P

D

D

A

L

D

D

A

T

V

W

Y

V

V

I

V

F

P

P

W

W

A

T

I

A

E

G

-

Q

-

G

P

K

T

V

A

A

I

R

V

L

I

I

H

C

D

D

T

V

F

Y

D

K

K

T

F

D

G

G

N

T

P

P

I

F

E

E

L

G

S

D

P

P

R

R

N

A

V

N

L

L

K

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binding adenosine-5'-diphosphate: G129 (≠ A138), E131 (= E140), E183 (≠ D200), D197 (≠ N214), F198 (= F215), K199 (≠ R216), Y200 (≠ H217), N246 (≠ H263), V247 (≠ Q264), S248 (= S265), R320 (= R337), S328 (≠ A345), R330 (= R347)
binding magnesium ion: E131 (= E140), E131 (= E140), E133 (= E142), E188 (= E205), E195 (= E212), E195 (= E212), H244 (= H261), E332 (= E349)
binding l-methionine-s-sulfoximine phosphate: E131 (= E140), E133 (= E142), E188 (= E205), E195 (= E212), G240 (= G257), H244 (= H261), R297 (= R314), E303 (≠ S320), R315 (= R332)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 83% coverage: 75:450/452 of query aligns to 63:446/446 of P9WN37

query
sites
P9WN37

A

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K363 (≠ E376) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
30% identity, 82% coverage: 78:446/452 of query aligns to 66:437/443 of 7tf9S

query
sites
7tf9S

D

D

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M

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