SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
E35 (≠ Q36) binding
E76 (≠ Q75) binding
Y135 (= Y136) mutation to A: 300-fold decrease in activity with spermine as substrate. Retains the ability to inhibit PrprA activity.; mutation Y->C,F: 100-fold decrease in activity with spermine as substrate.; mutation to S: Loss of activity with spermine as substrate.

6e1xA Crystal structure of product-bound complex of spermidine/spermine n- acetyltransferase speg
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 6e1xA

query
sites
6e1xA

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T
x
W

L

F

Q

E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G
x
Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S
x
H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding N-[3-({4-[(3-aminopropyl)amino]butyl}amino)propyl]acetamide: W30 (≠ T34), Q85 (≠ G88), H121 (≠ S124)

Sites not aligning to the query:

binding N-[3-({4-[(3-aminopropyl)amino]butyl}amino)propyl]acetamide: 147, 148

6cx8A Crystal structure of spermidine/spermine n-acetyltransferase speg from vibrio cholerae in complex with manganese ions.
32% identity, 72% coverage: 21:145/173 of query aligns to 18:143/173 of 6cx8A

query
sites
6cx8A

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q
x
E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding manganese (ii) ion: E33 (≠ Q36), E75 (≠ Q75)

4r87A Crystal structure of spermidine n-acetyltransferase from vibrio cholerae in complex with coa and spermine (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 4r87A

query
sites
4r87A

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T
x
W

L

F

Q

E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M
x
I

G
x
I

V
x
I

A

A

V

P

A

E

W

H

Q
|
Q

G
|
G

K

K

G
|
G

V

F

G
x
A

S
x
R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S
x
H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L
|
L

Y
|
Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding coenzyme a: W30 (≠ T34), I86 (≠ M89), I87 (≠ G90), I88 (≠ V91), Q93 (= Q96), G94 (= G97), G96 (= G99), A98 (≠ G101), R99 (≠ S102), H121 (≠ S124), L132 (= L135), Y133 (= Y136)

4r57A Crystal structure of spermidine n-acetyltransferase from vibrio cholerae in complex with acetyl-coa (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 4r57A

query
sites
4r57A

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q
x
Y

T
x
W

L

F

Q

E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G
x
Q

M
x
I

G
x
I

V
x
I

A

A

V

P

A

E

W

H

Q
|
Q

G
|
G

K

K

G
|
G

V
x
F

G
x
A

S
x
R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S
x
H

V
|
V

Y

A

A

V

D

E

N
|
N

E

P

A
x
K

A

A

I

V

S

H

L
|
L

Y
|
Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding acetyl coenzyme *a: Y29 (≠ Q33), W30 (≠ T34), Q85 (≠ G88), I86 (≠ M89), I87 (≠ G90), I88 (≠ V91), Q93 (= Q96), G94 (= G97), G96 (= G99), F97 (≠ V100), A98 (≠ G101), R99 (≠ S102), H121 (≠ S124), V122 (= V125), N126 (= N129), K128 (≠ A131), L132 (= L135), Y133 (= Y136)

4nczA Spermidine n-acetyltransferase from vibrio cholerae in complex with 2- [n-cyclohexylamino]ethane sulfonate. (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 4nczA

query
sites
4nczA

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q
x
E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding calcium ion: E32 (≠ Q36), E74 (≠ Q75)

4mi4A Crystal structure of spermidine n-acetyltransferase from vibrio cholerae in complex with spermine (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 4mi4A

query
sites
4mi4A

V

I

A

H

A

N

L

L

Y
x
N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T

W

L

F

Q
x
E

M
x
E

P

P

F
x
Y

Q
x
E

S

S

-

F

-

D

-
x
E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding spermine: N21 (≠ Y25), E32 (≠ Q36), E33 (≠ M37), Y35 (≠ F39), E36 (≠ Q40), E40 (vs. gap)

4mhdA Crystal structure of spermidine n-acetyltransferase from vibrio cholerae in complex with spermidine (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/170 of 4mhdA

query
sites
4mhdA

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F
x
Y

Q

E

S

S

-

F

-

D

-
x
E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding spermidine: E32 (≠ Q36), Y35 (≠ F39), E40 (vs. gap)

Q9KL03 Spermidine N(1)-acetyltransferase; SAT; Spermidine/spermine N(1)-acetyltransferase; SSAT; EC 2.3.1.57 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see 2 papers)
32% identity, 72% coverage: 21:145/173 of query aligns to 18:143/173 of Q9KL03

query
sites
Q9KL03

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A
x
M

R

S

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-
x
E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R
x
H

L
x
I

A
x
H

L
x
D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q
x
E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S
x
E

F
|
F

G
x
Q

M
x
I

G
x
I

V
x
I

A

A

V

P

A

E

W

H

Q
|
Q

G
|
G

K
|
K

G
|
G

V
x
F

G
x
A

S
x
R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N
|
N

E
x
P

A
x
K

A
|
A

I
x
V

S
x
H

L
|
L

Y
|
Y

R
x
E

K
x
E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

M28 (≠ A31) binding
E33 (≠ Q36) binding ; binding ; binding
E41 (vs. gap) binding ; binding
HIHD 49:52 (≠ RLAL 48:51) binding
E75 (≠ Q75) binding
EFQ 84:86 (≠ SFG 86:88) binding
III 87:89 (≠ MGV 89:91) binding
94:100 (vs. 96:102, 57% identical) binding
127:136 (vs. 129:138, 40% identical) binding

6e1xC Crystal structure of product-bound complex of spermidine/spermine n- acetyltransferase speg
32% identity, 72% coverage: 21:145/173 of query aligns to 21:146/176 of 6e1xC

query
sites
6e1xC

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T
x
W

L

F

Q

E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q

E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G
x
Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding N-{3-[(4-aminobutyl)amino]propyl}acetamide: W34 (≠ T34), Q89 (≠ G88)

5cnpB X-ray crystal structure of spermidine n1-acetyltransferase from vibrio cholerae. (see paper)
32% identity, 72% coverage: 21:145/173 of query aligns to 17:142/171 of 5cnpB

query
sites
5cnpB

V

I

A

H

A

N

L

L

Y

N

N

N

D

N

P

R

A

N

I

I

A

M

R

S

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

S

-

F

-

D

-

E

-

L

T

E

E

E

L

L

W

Y

R

N

S

K

R

H

L

I

A

H

L

D

D

N

N

A

E

E

R

R

L

R

V

F

N

-

V

V

A

E

L

D

H

A

Q

Q

G

K

T

N

V

L

I

I

G

G

N

L

I

V

G

E

L

L

E

I

Q
x
E

F

I

S

N

R

Y

I

I

R

H

R

R

S

S

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

A

V

P

A

E

W

H

Q

Q

G

G

K

K

G

G

V

F

G

A

S

R

K

T

L

L

L

I

A

N

T

R

A

A

L

L

D

D

I

Y

A

S

D

F

N

T

W

I

M

L

N

N

L

L

Q

H

R

K

I

I

E

Y

L

L

S

H

V

V

Y

A

A

V

D

E

N

N

E

P

A

K

A

A

I

V

S

H

L

L

Y

Y

R

E

K

E

F

C

G

G

F

F

E

V

T

E

E

E

G

G

binding magnesium ion: E32 (≠ Q36), E74 (≠ Q75)

3wr7A Crystal structure of spermidine acetyltransferase from escherichia coli (see paper)
27% identity, 72% coverage: 21:145/173 of query aligns to 16:140/170 of 3wr7A

query
sites
3wr7A

V

V

A

H

A

Q

L

L

Y

D

N

N

D

N

P

A

A

S

I

V

A

M

R

R

Q
x
Y

T

W

L

F

Q
x
E

M

E

P

P

F
x
Y

Q

E

S

A

-

F

-

V

-
x
E

-

L

T

S

E

D

L

L

W

Y

R

D

S

K

R

H

L

I

A

H

L

D

D

Q

N

S

E

E

R

R

L

R

V

F

N

V

V

V

V

E

A

C

L

-

H

-

Q

D

G

G

T

E

V

K

I

A

G

G

N

L

I

V

G

E

L

L

E

V

Q

E

F

I

S

N

R

H

I

V

R

H

R

R

S

R

H

-

A

-

G

A

S

E

F

F

G
x
Q

M
x
I

G

I

V

I

A

S

V

P

A

E

W

Y

Q
|
Q

G
|
G

K
|
K

G
|
G

V
x
L

G
x
A

S

T

K

R

L

A

A

K

T

L

A

A

L

M

D

D

I

Y

A

G

D

F

N

T

W

V

M

L

N

N

L

L

Q

Y

R

K

I

L

E

Y

L

L

S
x
I

V

V

Y

D

A

K

D

E

N

N

E

E

A
x
K

A

A

I

I

S

H

L

I

Y
|
Y

R

R

K

K

F

L

G

G

F

F

E

S

T

V

E

E

G

G

binding coenzyme a: Y28 (≠ Q33), Q83 (≠ G88), I84 (≠ M89), Q91 (= Q96), G92 (= G97), K93 (= K98), G94 (= G99), L95 (≠ V100), A96 (≠ G101), I119 (≠ S124), K126 (≠ A131), Y131 (= Y136)
binding spermidine: E31 (≠ Q36), Y34 (≠ F39), E39 (vs. gap)

6d72B Crystal structure of spermidine/spermine n-acetyltransferase speg from yersinia pestis in complex with calcium ions.
29% identity, 72% coverage: 21:145/173 of query aligns to 21:145/176 of 6d72B

query
sites
6d72B

V

V

A

H

A

Q

L

L

Y

D

N

N

D

N

P

A

A

S

I

I

A

M

R

R

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

A

-

F

-

V

-

E

-

L

T

C

E

D

L

L

W

Y

R

D

S

K

R

H

L

I

A

H

L

D

D

Q

N

S

E

E

R

R

L

-

V

-

N

R

V

F

V

I

A

I

L

E

H

S

Q

Q

G

G

T

T

V

K

I

I

G

G

N

L

I

V

G

E

L

L

E

V

Q
x
E

F

I

S

N

R

H

I

I

R

H

R

R

S

R

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

D

V

P

A

T

W

H

Q

Q

G

G

K

K

G

G

V

Y

G

A

S

G

K

A

L

A

A

K

T

L

A

A

L

M

D

E

I

Y

A

G

D

F

N

S

W

V

M

L

N

N

L

L

Q

Y

R

K

I

L

E

Y

L

L

S

I

V

V

Y

D

A

K

D

E

N

N

E

E

A

K

A

A

I

I

S

H

L

I

Y

Y

R

S

K

K

F

L

G

G

F

F

E

E

T

I

E

E

G

G

binding calcium ion: E36 (≠ Q36), E77 (≠ Q75)

P0A948 [Ribosomal protein uS5]-alanine N-acetyltransferase; Acetylating enzyme for N-terminal of ribosomal protein S5; EC 2.3.1.267 from Escherichia coli (strain K12) (see paper)
29% identity, 69% coverage: 45:164/173 of query aligns to 59:184/194 of P0A948

query
sites
P0A948

W

W

R

Q

S

A

R

R

L

L

A

G

L

M

D

I

N

N

E

E

R

F

L

H

V

K

N

Q

V

G

V

S

A

A

L

F

H

Y

Q

F

G

G

T

L

V

F

I

D

G

P

N

D

-

E

-

K

-

E

-

I

I

I

G

G

L

V

E

A

Q

N

F

F

S

S

R

N

I

V

R

V

R

R

S

G

-

S

-

F

H

H

A

A

G
x
C

S

Y

F

L

G

G

M

Y

G

S

V

I

A

G

V

Q

A

K

W

W

Q

Q

G

G

K

K

G

G

V

L

G

M

S

F

K

E

L

A

L

L

A

T

T

A

A

A

L

I

D

R

I

Y

A

M

D

Q

N

R

W

T

M

Q

N

H

L

I

Q

H

R

R

I

I

E

M

L

A

S

N

V

Y

Y

M

A

P

D

H

N

N

E

K

A

R

A

S

I

G

S

D

L

L

Y

L

R

A

K

R

F

L

G

G

F

F

E

E

T

K

E

E

G

G

L

Y

F

A

R

K

D

D

Y

Y

A

L

V

L

R

I

D

D

G

G

V

Q

L

W

V

R

D

D

T

H

L

V

S

L

M

T

A

A

C105 (≠ G85) mutation to A: Loss of acetyltransferase activity, can still suppress uS5(G28D) defects; when associated with A-54.

Sites not aligning to the query:

54 C→A: Loss of acetyltransferase activity, can still suppress uS5(G28D) defects; when associated with A-105.; C→F: Loss of acetyltransferase activity.

6yzzA Arabidopsis thaliana naa50 in complex with accoa (see paper)
31% identity, 57% coverage: 60:158/173 of query aligns to 45:147/151 of 6yzzA

query
sites
6yzzA

V

L

A

A

L

Y

H

Y

Q

N

G

D

T

I

V

C

I

V

G

G

N

A

I

I

G

A

L

-

E

-

Q

-

F

-

S

C

R

R

I

L

R

E

R

K

S

K

H

E

A

S

G

G

S

A

F

M

-

R

-

V

-

Y

-

I

-

M

G

T

M

L

G
|
G

V
|
V

A

L

V

A

A

P

W

Y

Q
x
R

G
|
G

K

I

G
|
G

V

I

G
|
G

S
|
S

K

N

L

L

A

N

T

H

A

V

L

L

D

D

I

M

A

C

D

S

N

K

W

-

M

Q

N

N

L

M

Q

C

R

E

I

I

E

Y

L
|
L

S

H

V

V

Y

Q

A

T

D

N

N

N

E

E

A

D

A

A

I

I

S

K

L
x
F

Y
|
Y

R

K

K
|
K

F

F

G

G

F

F

E

E

T

I

E

T

G

D

L

T

F

I

R

Q

D

N

Y

Y

A

Y

V

I

-

N

-

I

-

E

-

P

R

R

D

D

G

C

V

Y

L

V

V

V

binding acetyl coenzyme *a: G76 (= G90), V77 (= V91), R82 (≠ Q96), G83 (= G97), G85 (= G99), G87 (= G101), S88 (= S102), L108 (= L123), F120 (≠ L135), Y121 (= Y136), K123 (= K138)

2i79A The crystal structure of the acetyltransferase of gnat family from streptococcus pneumoniae
30% identity, 66% coverage: 52:166/173 of query aligns to 57:170/171 of 2i79A

query
sites
2i79A

D

D

N

N

E

Q

R

-

L

-

V

I

N

T

V

L

A

A

L

F

H

L

Q

N

G

G

T

K

V

I

I

A

G

G

N

I

I

V

G

N

L

I

E

T

Q

A

F

D

S

Q

R

R

I

K

R

R

R

V

S

R

H

H

A

I

G

G

S

D

F

L

G
x
F

M
x
I

G
x
V

V
x
I

A

G

V

K

A

R

W

Y

Q
x
W

G
x
N

K

N

G
|
G

V

L

G
|
G

S
|
S

K

L

L

L

A

E

T

E

A

A

L

I

D

E

I

W

A

A

D

Q

N

A

W

S

M

G

N

I

L

L

Q

R

R

R

I

L

E

Q

L
|
L

S

T

V

V

Y

Q

A

T

D

R

N

N

E
x
Q

A
|
A

A

A

I

V

S
x
H

L

L

Y
|
Y

R

Q

K

K

F

H

G

G

F

F

E

V

T

I

E

E

G

G

-

S

L

Q

F

E

R

R

D

G

Y

A

A

Y

V

I

R

E

D

E

G

G

V

K

L

F

V

I

D

D

T

V

L

Y

S

L

M

M

A

G

R

K

L

L

binding acetyl coenzyme *a: F91 (≠ G88), I92 (≠ M89), V93 (≠ G90), I94 (≠ V91), W99 (≠ Q96), N100 (≠ G97), G102 (= G99), G104 (= G101), S105 (= S102), L126 (= L123), Q133 (≠ E130), A134 (= A131), H137 (≠ S134), Y139 (= Y136)

Sites not aligning to the query:

binding acetyl coenzyme *a: 32

6z00A Arabidopsis thaliana naa50 in complex with bisubstrate analogue coa- ac-mvnal (see paper)
31% identity, 57% coverage: 60:158/173 of query aligns to 50:152/156 of 6z00A

query
sites
6z00A

V

L

A

A

L

Y

H

Y

Q

N

G

D

T

I

V

C

I

V

G

G

N

A

I

I

G

A

L

-

E

-

Q

-

F

-

S

C

R

R

I

L

R

E

R

K

S

K

H

E

A

S

G

G

S

A

F

M

-

R

-

V

-

Y

-

I

-
x
M

G
x
T

M
x
L

G
|
G

V
|
V

A

L

V

A

A

P

W

Y

Q
x
R

G
|
G

K

I

G
|
G

V

I

G
|
G

S
|
S

K

N

L

L

A

N

T

H

A

V

L

L

D

D

I

M

A

C

D

S

N

K

W

-

M

Q

N

N

L

M

Q

C

R

E

I

I

E

Y

L

L

S
x
H

V
|
V

Y

Q

A

T

D

N

N

N

E

E

A
x
D

A

A

I

I

S

K

L
x
F

Y
|
Y

R

K

K

K

F

F

G

G

F

F

E

E

T

I

E

T

G

D

L

T

F

I

R

Q

D

N

Y
|
Y

A
x
Y

V

I

-

N

-

I

-

E

-

P

R

R

D

D

G

C

V

Y

L

V

V

V

binding carboxymethyl coenzyme *a: T79 (≠ G88), L80 (≠ M89), G81 (= G90), V82 (= V91), R87 (≠ Q96), G88 (= G97), G90 (= G99), G92 (= G101), S93 (= S102), H114 (≠ S124), V115 (= V125), D121 (≠ A131), F125 (≠ L135), Y126 (= Y136)
binding : M78 (vs. gap), Y140 (= Y150), Y141 (≠ A151)

Sites not aligning to the query:

binding : 29, 31, 33, 36

4r9mA Crystal structure of spermidine n-acetyltransferase from escherichia coli (see paper)
26% identity, 68% coverage: 29:145/173 of query aligns to 25:141/169 of 4r9mA

query
sites
4r9mA

A

S

I

V

A

M

R

R

Q

Y

T

W

L

F

Q
x
E

M

E

P

P

F

Y

Q

E

S

A

-

F

-

V

-

E

-

L

T

S

E

D

L

L

W

Y

R

D

S

K

R

H

L

I

A

H

L

D

D

Q

N

S

E

E

R

R

L

-

V

-

N

R

V

F

V

V

A

V

L

E

H

C

Q

D

G

G

T

E

V

K

I

A

G

G

N

L

I

V

G

E

L

L

E

V

Q
x
E

F

I

S

N

R

H

I

V

R

H

R

R

S

R

H

-

A

-

G

A

S

E

F

F

G

Q

M

I

G

I

V

I

A

S

V

P

A

E

W

Y

Q

Q

G

G

K

K

G

G

V

L

G

A

S

T

K

R

L

A

A

K

T

L

A

A

L

M

D

D

I

Y

A

G

D

F

N

T

W

V

M

L

N

N

L

L

Q

Y

R

K

I

L

E

Y

L

L

S

I

V

V

Y

D

A

K

D

E

N

N

E

E

A

K

A

A

I

I

S

H

L

I

Y

Y

R

R

K

K

F

L

G

G

F

F

E

S

T

V

E

E

G

G

binding magnesium ion: E32 (≠ Q36), E73 (≠ Q75)

Query Sequence

>PfGW456L13_3289 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3289
MPATEPVITLERFNESHIEGVAALYNDPAIARQTLQMPFQSTELWRSRLALDNERLVNVV
ALHQGTVIGNIGLEQFSRIRRSHAGSFGMGVAVAWQGKGVGSKLLATALDIADNWMNLQR
IELSVYADNEAAISLYRKFGFETEGLFRDYAVRDGVLVDTLSMARLRRTPKAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory