SitesBLAST
Comparing PfGW456L13_3458 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
37% identity, 99% coverage: 1:249/251 of query aligns to 1:247/247 of 4jroC
- active site: G16 (= G16), S142 (= S135), Q152 (≠ K145), Y155 (= Y148), K159 (= K152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G12), S14 (≠ A14), R15 (= R15), G16 (= G16), I17 (= I17), N35 (≠ L35), Y36 (≠ D36), N37 (≠ L37), G38 (= G38), S39 (vs. gap), N63 (≠ D57), V64 (≠ I58), N90 (≠ A84), A91 (= A85), I93 (= I87), I113 (≠ V107), S142 (= S135), Y155 (= Y148), K159 (= K152), P185 (= P178), I188 (≠ W181), T190 (≠ P183)
3ak4A Crystal structure of nadh-dependent quinuclidinone reductase from agrobacterium tumefaciens
38% identity, 99% coverage: 1:249/251 of query aligns to 3:257/258 of 3ak4A
- active site: G18 (= G16), S141 (= S135), L151 (≠ K145), Y154 (= Y148), K158 (= K152), E199 (≠ D186)
- binding nicotinamide-adenine-dinucleotide: K17 (≠ R15), G18 (= G16), I19 (= I17), D38 (≠ S34), L39 (= L35), V60 (≠ C56), D61 (= D57), V62 (≠ I58), N88 (≠ A84), A89 (= A85), G90 (= G86), T139 (≠ F133), S141 (= S135), Y154 (= Y148), K158 (= K152), G185 (≠ A179), V187 (≠ W181), T189 (≠ P183), M191 (vs. gap)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
39% identity, 99% coverage: 1:249/251 of query aligns to 4:247/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (= R15), G19 (= G16), I20 (= I17), D39 (= D36), R40 (≠ L37), C63 (= C56), I65 (= I58), N91 (≠ A84), G93 (= G86), I94 (= I87), V114 (= V107), Y155 (= Y148), K159 (= K152), I188 (≠ W181), T190 (≠ P183), T193 (= T188)
8cxaA Crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from mycobacterium smegmatis with bound NAD
38% identity, 98% coverage: 1:247/251 of query aligns to 1:248/251 of 8cxaA
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), Q15 (≠ R15), G16 (= G16), I17 (= I17), D36 (= D36), V63 (≠ I58), N89 (≠ A84), A91 (≠ G86), S94 (≠ P89), I142 (≠ F133), S143 (≠ A134), S144 (= S135), Y157 (= Y148), K161 (= K152), P187 (= P178), H188 (≠ W181), I190 (≠ P183), I194 (≠ A187)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
38% identity, 97% coverage: 6:249/251 of query aligns to 3:255/256 of Q48436
- 6:33 (vs. 9:36, 39% identical) binding
- D59 (= D57) binding
- K156 (= K152) binding
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
38% identity, 97% coverage: 6:249/251 of query aligns to 3:255/256 of 1gegE
- active site: G13 (= G16), S139 (= S135), Y152 (= Y148), K156 (= K152), V197 (≠ R189)
- binding alpha-D-glucopyranose: R63 (≠ S61), D64 (≠ Q62), F67 (≠ D65), E123 (= E121)
- binding nicotinamide-adenine-dinucleotide: G9 (= G12), Q12 (≠ R15), I14 (= I17), D33 (= D36), Y34 (≠ L37), V58 (≠ C56), D59 (= D57), V60 (≠ I58), N86 (≠ A84), A87 (= A85), I109 (≠ V107), S139 (= S135), Y152 (= Y148), K156 (= K152), P182 (= P178), V185 (vs. gap), T187 (= T182), M189 (= M184)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 99% coverage: 2:249/251 of query aligns to 4:244/244 of 4nbuB
- active site: G18 (= G16), N111 (= N108), S139 (= S135), Q149 (≠ K145), Y152 (= Y148), K156 (= K152)
- binding acetoacetyl-coenzyme a: D93 (≠ N90), K98 (≠ S95), S139 (= S135), N146 (≠ Q142), V147 (≠ P143), Q149 (≠ K145), Y152 (= Y148), F184 (≠ M180), M189 (= M192), K200 (≠ Y203)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ R15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ L37), V59 (= V55), D60 (= D57), V61 (≠ I58), N87 (≠ A84), A88 (= A85), G89 (= G86), I90 (= I87), T137 (≠ F133), S139 (= S135), Y152 (= Y148), K156 (= K152), P182 (= P178), F184 (≠ M180), T185 (= T188), T187 (≠ A190), M189 (= M192)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
34% identity, 100% coverage: 1:250/251 of query aligns to 4:243/243 of 4i08A
- active site: G19 (= G16), N113 (= N108), S141 (= S135), Q151 (≠ K145), Y154 (= Y148), K158 (= K152)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (= I17), T40 (≠ L37), N62 (≠ D57), V63 (≠ I58), N89 (≠ A84), A90 (= A85), G140 (≠ A134), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (≠ A179), T189 (= T182)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
34% identity, 100% coverage: 1:250/251 of query aligns to 4:247/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G12), S17 (≠ A14), R18 (= R15), I20 (= I17), T40 (≠ L37), N62 (≠ D57), V63 (≠ I58), N89 (≠ A84), A90 (= A85), I92 (= I87), V139 (≠ F133), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (≠ A179), I187 (≠ W181), T189 (≠ P183), M191 (≠ A187)
3rwbA Crystal structure of complex of 4pal (4-pyridoxolactone) and pldh (tetrameric pyridoxal 4-dehydrogenase) from mesorhizobium loti
37% identity, 98% coverage: 2:248/251 of query aligns to 3:245/247 of 3rwbA
- active site: G17 (= G16), S140 (= S135), Y153 (= Y148), K157 (= K152)
- binding 7-hydroxy-6-methylfuro[3,4-c]pyridin-1(3H)-one: S140 (= S135), N141 (≠ A136), T142 (≠ A137), M150 (≠ K145), Y153 (= Y148), L185 (≠ M180), H196 (≠ S193)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), Q16 (≠ R15), G17 (= G16), I18 (= I17), D37 (= D36), I38 (≠ L37), D60 (= D57), I61 (= I58), N87 (≠ A84), A88 (= A85), S89 (≠ G86), I138 (≠ F133), S140 (= S135), Y153 (= Y148), K157 (= K152), P183 (= P178), L185 (≠ M180), I186 (≠ W181), S188 (≠ P183), G190 (≠ A187), V191 (≠ T188)
3ndrA Crystal structure of tetrameric pyridoxal 4-dehydrogenase from mesorhizobium loti
37% identity, 98% coverage: 2:248/251 of query aligns to 3:245/247 of 3ndrA
- active site: G17 (= G16), S140 (= S135), Y153 (= Y148), K157 (= K152)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), Q16 (≠ R15), G17 (= G16), I18 (= I17), D37 (= D36), I38 (≠ L37), D60 (= D57), I61 (= I58), N87 (≠ A84), A88 (= A85), S89 (≠ G86), V110 (= V107), I138 (≠ F133), S140 (= S135), Y153 (= Y148), K157 (= K152), P183 (= P178), L185 (≠ M180), I186 (≠ W181), S188 (≠ P183), G190 (≠ A187), V191 (≠ T188)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
36% identity, 98% coverage: 1:246/251 of query aligns to 2:248/252 of 1vl8B
- active site: G17 (= G16), S143 (= S135), I154 (≠ K145), Y157 (= Y148), K161 (= K152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G12), R16 (= R15), G17 (= G16), L18 (≠ I17), S37 (= S34), R38 (≠ L35), C63 (= C56), D64 (= D57), V65 (≠ I58), A91 (= A84), A92 (= A85), G93 (= G86), I94 (= I87), V114 (= V107), I141 (≠ F133), S143 (= S135), Y157 (= Y148), K161 (= K152), P187 (= P178), G188 (≠ A179), Y190 (≠ W181), T192 (= T182), M194 (= M184), T195 (≠ Y185)
7wbcA Hydroxysteroid dehydrogenase wild-type complexed with NAD+ and (4s)-2- 2-methyl-2,4-pentanediol
37% identity, 100% coverage: 1:250/251 of query aligns to 1:249/250 of 7wbcA
- binding calcium ion: Y115 (≠ R110), P116 (≠ G111), H119 (≠ L114)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), G16 (= G16), I17 (= I17), D36 (= D36), V37 (≠ L37), A61 (≠ C56), D62 (= D57), I63 (= I58), N89 (≠ A84), F138 (= F133), S140 (= S135), Y153 (= Y148), K157 (= K152), P183 (= P178), F184 (≠ Y185), A185 (≠ D186), T187 (= T188), G189 (≠ A190), V190 (≠ S191)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
34% identity, 98% coverage: 5:250/251 of query aligns to 4:243/243 of 7emgB
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 98% coverage: 1:246/251 of query aligns to 3:251/255 of 5itvA
- active site: G18 (= G16), S141 (= S135), Y154 (= Y148), K158 (= K152)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (≠ R15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (≠ L37), T61 (≠ C56), I63 (= I58), N89 (≠ A84), G91 (= G86), T139 (≠ F133), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (≠ A179), I186 (≠ M180), I187 (≠ W181)
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
39% identity, 98% coverage: 2:247/251 of query aligns to 8:252/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G12), S20 (≠ A14), K21 (≠ R15), G22 (= G16), I23 (= I17), A43 (vs. gap), S44 (= S34), S45 (≠ L35), G68 (≠ C56), D69 (= D57), V70 (≠ I58), N96 (≠ A84), S97 (≠ A85), G98 (= G86), Y100 (≠ A88), I144 (≠ F133), S146 (= S135), Y159 (= Y148), K163 (= K152), P189 (= P178), G190 (≠ A179), M191 (= M180), I192 (≠ W181), T194 (= T188), G196 (≠ A190), T197 (≠ S191)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S135), Y159 (= Y148), M191 (= M180), I202 (≠ Q196)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
34% identity, 97% coverage: 6:249/251 of query aligns to 5:246/246 of 3osuA
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
34% identity, 99% coverage: 2:249/251 of query aligns to 2:239/239 of 4nbtA
- active site: G16 (= G16), S132 (= S135), Y145 (= Y148), K149 (= K152)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ R15), G16 (= G16), L17 (≠ I17), D36 (= D36), L37 (= L37), L52 (≠ C56), N53 (≠ D57), V54 (≠ I58), N80 (≠ A84), A81 (= A85), G82 (= G86), I130 (≠ F133), S132 (= S135), Y145 (= Y148), K149 (= K152), P177 (= P178), G178 (≠ A179), I180 (≠ W181), T182 (≠ Y185)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
35% identity, 99% coverage: 3:250/251 of query aligns to 4:247/247 of P73574
- A14 (≠ G13) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (= P143) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K152) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ M180) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ S193) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 100% coverage: 1:250/251 of query aligns to 1:244/244 of P0A2C9
- M125 (≠ L122) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (= A229) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (≠ G230) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
Query Sequence
>PfGW456L13_3458 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
MQLAGKRIIVTGGARGIGAAVVKAFVEEGAHVVSLDLGEAASITGDGGGWAHSRVCDIAD
SQSVDAAFAWASEQLNGLDVLVHAAGIAPNASADSITLDEWEKVFAVNTRGTFLTNRAAY
ELLKGSGGRIINFASAAGVLGQPGKAHYAASKGAVLAWTRTVAREWGPLGITVNAIAPAM
WTPMYDATRASMSAEQLQQHDAYMAGQVPIGGKLGEPARDLAPVLVFLAGDGSRFVTGQT
LVIDGGMMMLS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory