SitesBLAST
Comparing PfGW456L13_3585 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3585 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1eyyA Crystal structure of the NADP+ dependent aldehyde dehydrogenase from vibrio harveyi. (see paper)
50% identity, 89% coverage: 58:522/525 of query aligns to 42:502/504 of 1eyyA
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
31% identity, 56% coverage: 145:437/525 of query aligns to 139:408/490 of Q9HTJ1
- GAWN 150:153 (≠ GASN 156:159) binding
- K162 (≠ D170) active site, Charge relay system
- KPSE 176:179 (≠ KAHG 184:187) binding
- G209 (= G221) binding
- GTST 230:233 (≠ SRSG 242:245) binding
- E252 (= E265) active site, Proton acceptor
- C286 (= C302) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (≠ H416) binding
Sites not aligning to the query:
- 464 active site, Charge relay system
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
31% identity, 56% coverage: 145:437/525 of query aligns to 138:407/489 of 4cazA
- active site: N152 (= N159), K175 (= K184), E251 (= E265), C285 (= C302), E386 (≠ H416)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ F155), G149 (= G156), W151 (≠ S158), N152 (= N159), K175 (= K184), E178 (≠ G187), G208 (= G221), G212 (= G225), F226 (= F239), T227 (= T240), G228 (= G241), G229 (≠ S242), T232 (≠ G245), V236 (≠ L249), E251 (= E265), L252 (≠ M266), C285 (= C302), E386 (≠ H416), F388 (≠ E418)
Sites not aligning to the query:
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
31% identity, 56% coverage: 145:437/525 of query aligns to 138:407/489 of 2woxA
- active site: N152 (= N159), K175 (= K184), E251 (= E265), C285 (= C302), E386 (≠ H416)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (≠ F155), G149 (= G156), W151 (≠ S158), N152 (= N159), K175 (= K184), S177 (≠ H186), E178 (≠ G187), G208 (= G221), G212 (= G225), F226 (= F239), T227 (= T240), G228 (= G241), G229 (≠ S242), T232 (≠ G245), V236 (≠ L249), E251 (= E265), L252 (≠ M266), C285 (= C302), E386 (≠ H416), F388 (≠ E418)
Sites not aligning to the query:
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
31% identity, 56% coverage: 145:437/525 of query aligns to 138:407/489 of 2wmeA
- active site: N152 (= N159), K175 (= K184), E251 (= E265), C285 (= C302), E386 (≠ H416)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (= G156), W151 (≠ S158), K175 (= K184), S177 (≠ H186), E178 (≠ G187), G208 (= G221), G212 (= G225), F226 (= F239), G228 (= G241), G229 (≠ S242), T232 (≠ G245), V236 (≠ L249)
Sites not aligning to the query:
3rhdA Crystal structure of glyceraldehyde-3-phosphate dehydrogenase gapn from methanocaldococcus jannaschii dsm 2661 complexed with NADP
25% identity, 66% coverage: 17:360/525 of query aligns to 2:318/456 of 3rhdA
- active site: N133 (= N159), H156 (≠ K184), E233 (= E265), C267 (= C302)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I129 (≠ F155), T130 (≠ G156), F132 (≠ S158), H156 (≠ K184), S158 (≠ H186), S159 (≠ G187), K160 (≠ A188), G193 (= G221), E194 (≠ R222), G197 (= G225), D198 (≠ I226), F211 (= F239), S214 (= S242), V217 (≠ G245)
Sites not aligning to the query:
3ju8A Crystal structure of succinylglutamic semialdehyde dehydrogenase from pseudomonas aeruginosa.
29% identity, 58% coverage: 9:313/525 of query aligns to 7:290/486 of 3ju8A
- active site: N147 (= N159), K170 (= K184), E245 (= E265), C279 (= C302)
- binding nicotinamide-adenine-dinucleotide: G144 (= G156), Y146 (≠ S158), N147 (= N159), L152 (≠ V166), K170 (= K184), S172 (≠ H186), F220 (= F239), T221 (= T240), G222 (= G241), S223 (= S242), T226 (≠ G245), E245 (= E265), M246 (= M266), G247 (≠ S267), C279 (= C302)
Sites not aligning to the query:
2d4eC Crystal structure of the hpcc from thermus thermophilus hb8
30% identity, 57% coverage: 6:303/525 of query aligns to 29:306/515 of 2d4eC
- active site: N173 (= N159), K196 (= K184), E271 (= E265), C305 (= C302)
- binding nicotinamide-adenine-dinucleotide: I169 (≠ F155), T170 (≠ G156), P171 (≠ A157), W172 (≠ S158), K196 (= K184), A198 (≠ H186), G229 (= G221), G233 (= G225), A234 (≠ I226), T248 (= T240), G249 (= G241), E250 (vs. gap), T253 (≠ S244), E271 (= E265), L272 (≠ M266), C305 (= C302)
Sites not aligning to the query:
P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
23% identity, 80% coverage: 11:429/525 of query aligns to 17:426/497 of P17202
- I28 (= I24) binding
- D96 (≠ E91) binding
- SPW 156:158 (≠ GAS 156:158) binding
- Y160 (≠ F160) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-(trimethylamino)butanal.
- W167 (≠ G169) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- KP-------SE 182:185 (≠ KAHGAHPGTSE 184:194) binding
- L186 (= L195) binding
- SSAT 236:239 (≠ SR-S 242:244) binding
- V251 (≠ R256) binding in other chain
- L258 (≠ M266) binding
- W285 (≠ Q296) mutation to A: Decreases binding affinity for betaine aldehyde.
- E390 (= E393) binding
Sites not aligning to the query:
- 441 A→I: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- 450 C→S: Loss of partial inactivation by betaine aldehyde in the absence of NAD(+).
- 456 binding ; W→A: Decreases binding affinity for betaine aldehyde.
- 460 binding
5ur2B Crystal structure of proline utilization a (puta) from bdellovibrio bacteriovorus inactivated by n-propargylglycine (see paper)
25% identity, 84% coverage: 53:494/525 of query aligns to 520:945/959 of 5ur2B
Sites not aligning to the query:
- binding N-propargylglycine-modified flavin adenine dinucleotide: 174, 215, 216, 249, 278, 279, 280, 281, 283, 300, 301, 302, 303, 306, 329, 330, 331, 332, 356, 357, 358, 379, 398, 403, 405
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
27% identity, 63% coverage: 1:329/525 of query aligns to 1:315/482 of P25526
Sites not aligning to the query:
4v37A Crystal structure of betaine aldehyde dehydrogenase from spinach showing a thiohemiacetal with 3-aminopropionaldehyde
29% identity, 50% coverage: 11:275/525 of query aligns to 15:265/495 of 4v37A
- active site: N157 (= N159), K180 (= K184), E255 (= E265)
- binding nicotinamide-adenine-dinucleotide: I153 (≠ F155), S154 (≠ G156), P155 (≠ A157), W156 (≠ S158), N157 (= N159), M162 (≠ V166), K180 (= K184), S182 (= S193), E183 (= E194), G213 (= G221), G217 (= G225), A218 (≠ I226), T232 (= T240), G233 (= G241), S234 (= S242), T237 (≠ S244), E255 (= E265), L256 (≠ M266)
Sites not aligning to the query:
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
27% identity, 62% coverage: 7:329/525 of query aligns to 13:314/481 of 3jz4A
- active site: N156 (= N159), K179 (= K184), E254 (= E265), C288 (= C302)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (≠ A157), W155 (≠ S158), K179 (= K184), A181 (≠ H186), S182 (≠ G187), A212 (≠ G221), G216 (= G225), G232 (= G241), S233 (= S242), I236 (≠ G245), C288 (= C302)
Sites not aligning to the query:
Q84LK3 Betaine aldehyde dehydrogenase 2; OsBADH2; EC 1.2.1.8 from Oryza sativa subsp. japonica (Rice) (see paper)
24% identity, 66% coverage: 66:409/525 of query aligns to 74:409/503 of Q84LK3
- N162 (= N159) mutation to A: Slightly reduced affinity for NAD, 4-fold enhanced affinity for betaine aldehyde (Bet-ald), but 3-fold reduction in gamma-4-aminobutyraldehyde (GAB-ald) affinity and reduced catalytic efficiency (2-fold for Bet-ald and 8-fold for GAB-ald).
- W170 (≠ G169) mutation to A: Slightly reduced affinity for NAD, 4-fold enhanced affinity for betaine aldehyde (Bet-ald), but 2-fold reduction in gamma-4-aminobutyraldehyde (GAB-ald) affinity and reduced catalytic efficiency (2.5-fold for Bet-ald and 6-fold for GAB-ald).; mutation to F: Slightly reduced affinity for NAD, 5-fold enhanced affinity for betaine aldehyde (Bet-ald), but 3-fold reduction in gamma-4-aminobutyraldehyde (GAB-ald) affinity and 1.5-fold increase in catalytic efficiency towards gamma-aminobutyraldehyde (GAB-ald).
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
29% identity, 56% coverage: 10:304/525 of query aligns to 20:295/497 of 8skfA
- binding calcium ion: T33 (≠ G23), I34 (= I24), D100 (≠ E91), V187 (≠ A188)
- binding nicotinamide-adenine-dinucleotide: I156 (≠ F155), G157 (= G156), A158 (= A157), W159 (≠ S158), K183 (= K184), E186 (≠ G187), G216 (= G221), G220 (= G225), T235 (= T240), G236 (= G241), G237 (≠ S242), S240 (= S244), K243 (≠ I247), E259 (= E265), C293 (= C302)
Sites not aligning to the query:
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
29% identity, 56% coverage: 10:304/525 of query aligns to 11:286/488 of 8vr1A
Sites not aligning to the query:
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
29% identity, 56% coverage: 10:304/525 of query aligns to 11:286/488 of 8vr0A
Sites not aligning to the query:
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
29% identity, 56% coverage: 10:304/525 of query aligns to 11:286/488 of 8vqzA
Sites not aligning to the query:
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
29% identity, 56% coverage: 10:304/525 of query aligns to 11:286/488 of 8vqwC
- binding coenzyme a: I147 (≠ F155), W150 (≠ S158), K174 (= K184), S176 (≠ H186), E177 (≠ G187), G207 (= G221), G211 (= G225), F225 (= F239), G227 (= G241), G228 (≠ S242), S231 (= S244)
Sites not aligning to the query:
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
29% identity, 56% coverage: 10:304/525 of query aligns to 11:286/488 of 8vj3A
Query Sequence
>PfGW456L13_3585 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3585
MTLTGNMLIGQQAIAGNREAIRGIDPATDLPLEPAYHGGSGEHVEQACALAWSALDSYRA
TSLEARAEFLETIASEIEALGDELIDRAVAETGLPRPRIQGERGRTCQQLRTFALTVRAG
EWLDVRVDSALPERQPLPRPDLRQRQVPLGPVAVFGASNFPLAFSVAGGDTASALAAGCP
VIVKAHGAHPGTSELVGRALARAVKLCGLHEGVFSLLFGSGREVGIALVTDPRIKAVGFT
GSRSGGIALCNAAQARPEPIPVYAEMSSINPVLLFPAALQSRAEALAQGFVASLTQGAGQ
FCTNPGLVIARQGPDLERFIQSAAQLLQRSPAQTMLTPGIFKAYEAGVGALAEHAHAETV
AVGLKGETPNQCQAHLFVTQASEFLADPALQAEMFGAASLIVQCASDEQIRQVIEHLEGQ
LTATLHLDDADVESARALLPILERKAGRLLVNGWPTGVEVCDAMVHGGPFPATSDARTTS
VGTAAILRFLRPVCYQDFPDALLPAALKHGNPLQLRRLLDGQREA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory