SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_3738 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3738 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00046 Cytochrome c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
42% identity, 77% coverage: 29:127/129 of query aligns to 7:105/109 of P00046

query
sites
P00046

D

D

T

E

R

K

R

K

G

G

A

A

E

S

V

L

F

F

A

K

T

T

E

R

C

C

S

A

V

Q

C

C

H

H

A

T

V

V

T

E

K

K

G

G

T

G

T

A

G

N

M

K

M

V

G

G

P

P

N

N

L

L

F

H

G

G

V

V

Y

F

G

G

R

R

K

K

S

T

G

G

S

Q

L

A

A

E

G

G

F

F

S

S

Y

Y

S

T

Q

E

A

A

M

N

R

R

D

D

K

K

D

G

V

I

D

T

W

W

Q

D

A

E

E

E

N

T

I

L

T

F

Q

A

L

Y

I

L

T

E

Q

N

P

P

Q
x
K

A

K

F

Y

V

I

P

P

G

G

T

T

Y

K

M

M

P

A

Y

F

M

A

G

G

L

F

A

K

S

K

A

P

D

A

D

D

R

R

Q

N

A

N

V

V

A

I

C

T

F

Y

L

L

K

K

E

K

K77 (≠ Q99) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00047 Cytochrome c from Thermomyces lanuginosus (Humicola lanuginosa) (see paper)
38% identity, 77% coverage: 29:127/129 of query aligns to 10:108/111 of P00047

query
sites
P00047

D

D

T

A

R

S

R

K

G

G

A

A

E

N

V

L

F

F

A

K

T

T

E

R

C
|
C

S

A

V

Q

C
|
C

H

H

A

S

V

V

T

E

K

Q

G

G

T

G

T

A

G

N

M

K

M

I

G

G

P

P

N

N

L

L

F

H

G

G

V

L

Y

F

G

G

R

R

K

K

S

T

G

G

S

S

L

V

A

E

G

G

F

Y

S

S

Y

Y

S

T

Q

D

A

A

M

N

R

K

D

Q

K

A

D

G

V

I

D

T

W

W

Q

N

A

E

E

D

N

T

I

L

T

F

Q

E

L

Y

I

L

T

E

Q

N

P

P

Q
x
K

A

K

F

F

V

I

P

P

G

G

T

T

Y

K

M

M

P

A

Y

F

M

G

G

G

L

L

A
x
K

S

K

A

N

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

E

C22 (= C41) binding covalent
C25 (= C44) binding covalent
K80 (≠ Q99) modified: N6,N6-dimethyllysine
K94 (≠ A113) modified: N6,N6,N6-trimethyllysine; partial

1criA The role of a conserved internal water molecule and its associated hydrogen bond network in cytochromE C (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 1criA

query
sites
1criA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G

G

P

P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
I

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L

F

I

L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding protoporphyrin ix containing fe: R18 (≠ E40), C19 (= C41), C22 (= C44), H23 (= H45), I40 (≠ V62), S45 (= S67), Y51 (≠ F73), Y53 (= Y75), T54 (≠ S76), I57 (≠ M79), W64 (= W86), M69 (≠ I91), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)

P62773 Cytochrome c from Brassica oleracea (Wild cabbage) (see paper)
39% identity, 77% coverage: 29:127/129 of query aligns to 10:108/111 of P62773

query
sites
P62773

D

N

T

S

R

K

R

A

G

G

A

E

E

K

V

I

F

F

A

K

T

T

E

K

C

C

S

A

V

Q

C

C

H

H

A

T

V

V

T

D

K

K

G

G

T

A

T

G

G

H

M

K

M

Q

G

G

P

P

N

N

L

L

F

N

G

G

V

L

Y

F

G

G

R

R

K

Q

S

S

G

G

S

T

L

T

A

A

G

G

F

Y

S

S

Y

Y

S

S

Q

A

A

A

M

N

R

K

D

N

K

K

D

A

V

V

D

E

W

W

Q

E

A

E

E

K

N

T

I

L

T

Y

Q

D

L

Y

I

L

T

L

Q

N

P

P

Q
x
K

A

K

F

Y

V

I

P

P

G

G

T

T

Y

K

M

M

P

V

Y

F

M

P

G

G

L

L

A
x
K

S

K

A

P

D

Q

D

D

R

R

Q

A

A

D

V

L

A

I

C

A

F

Y

L

L

K

K

E

E

K80 (≠ Q99) modified: N6,N6,N6-trimethyllysine
K94 (≠ A113) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

P62772 Cytochrome c from Brassica napus (Rape) (see paper)
39% identity, 77% coverage: 29:127/129 of query aligns to 10:108/111 of P62772

query
sites
P62772

D

N

T

S

R

K

R

A

G

G

A

E

E

K

V

I

F

F

A

K

T

T

E

K

C

C

S

A

V

Q

C

C

H

H

A

T

V

V

T

D

K

K

G

G

T

A

T

G

G

H

M

K

M

Q

G

G

P

P

N

N

L

L

F

N

G

G

V

L

Y

F

G

G

R

R

K

Q

S

S

G

G

S

T

L

T

A

A

G

G

F

Y

S

S

Y

Y

S

S

Q

A

A

A

M

N

R

K

D

N

K

K

D

A

V

V

D

E

W

W

Q

E

A

E

E

K

N

T

I

L

T

Y

Q

D

L

Y

I

L

T

L

Q

N

P

P

Q
x
K

A

K

F

Y

V

I

P

P

G

G

T

T

Y

K

M

M

P

V

Y

F

M

P

G

G

L

L

A
x
K

S

K

A

P

D

Q

D

D

R

R

Q

A

A

D

V

L

A

I

C

A

F

Y

L

L

K

K

E

E

K80 (≠ Q99) modified: N6,N6,N6-trimethyllysine
K94 (≠ A113) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine
109 A → S: in 40% of the molecules

P00066 Cytochrome c from Nigella damascena (Love-in-a-mist) (see paper)
38% identity, 78% coverage: 27:127/129 of query aligns to 8:108/111 of P00066

query
sites
P00066

A

A

P

G

D

B

T

S

R

A

R

S

G

G

A

E

E

K

V

I

F

F

A

K

T

T

E

K

C
|
C

S

A

V

Z

C
|
C

H

H

A

T

V

V

T

B

K

Z

G

G

T

A

T

G

G

H

M

K

M

Z

G

G

P

P

N

N

L

L

F

H

G

G

V

L

Y

F

G

G

R

R

K

Q

S

S

G

G

S

T

L

V

A

A

G

G

F

Y

S

S

Y

Y

S

S

Q

A

A

A

M

N

R

K

D

N

K

K

D

A

V

V

D

N

W

W

Q

E

A

E

E

K

N

T

I

L

T

Y

Q

D

L

Y

I

L

T

L

Q

N

P

P

Q
x
K

A

K

F

Y

V

I

P

P

G

G

T

T

Y

K

M

M

P

V

Y

F

M

P

G

G

L

L

A
x
K

S

K

A

P

D

Z

D

Z

R

R

Q

A

A

B

V

L

A

L

C

A

F

Y

L

L

K

K

E

E

C22 (= C41) binding covalent
C25 (= C44) binding covalent
K80 (≠ Q99) modified: N6,N6,N6-trimethyllysine
K94 (≠ A113) modified: N6,N6,N6-trimethyllysine

Sites not aligning to the query:

1 modified: N-acetylalanine

7pr2A Cocrystal of cytochromE C and sulfonato-thiacalix[4]arene (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 7pr2A

query
sites
7pr2A

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V
x
Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W

W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A
x
K

S
x
K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding sulfonato-thiacalix[4]arene: R18 (≠ E40), K91 (≠ A113), K92 (≠ S114)
binding heme c: C19 (= C41), Q21 (≠ V43), C22 (= C44), H23 (= H45), G34 (= G56), P35 (= P57), I40 (≠ V62), S45 (= S67), G46 (= G68), Y51 (≠ F73), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)

6gd6A CytochromE C in complex with sulfonato-calix[8]arene, h3 form with ammonium sulfate (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 6gd6A

query
sites
6gd6A

A

A

P

G

D
x
S

T
x
A

R
x
K

R

K

G

G

A
|
A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C
x
T

F
x
Y

L

L

K

K

E
x
K

binding sulfonato-calix[8]arene: S7 (≠ D29), A8 (≠ T30), K9 (≠ R31), A12 (= A34), T101 (≠ C123), Y102 (≠ F124), K105 (≠ E127)
binding heme c: R18 (≠ E40), C19 (= C41), C22 (= C44), H23 (= H45), G34 (= G56), P35 (= P57), I40 (≠ V62), S45 (= S67), G46 (= G68), Y51 (≠ F73), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), W64 (= W86), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)

6egzA Crystal structure of cytochromE C in complex with di-pegylated sulfonatocalix[4]arene (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 6egzA

query
sites
6egzA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E

R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M
x
K

M
x
V

G
|
G

P
|
P

N

N

L
|
L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W

W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y

F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding di-PEGylated sulfonatocalix[4]arene: K32 (≠ M54), V33 (≠ M55)
binding heme c: C19 (= C41), C22 (= C44), H23 (= H45), G34 (= G56), P35 (= P57), L37 (= L59), I40 (≠ V62), S45 (= S67), Y51 (≠ F73), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107)

6egyA Crystal structure of cytochromE C in complex with mono-pegylated sulfonatocalix[4]arene (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 6egyA

query
sites
6egyA

A

A

P

G

D

S

T
x
A

R
x
K

R

K

G

G

A
|
A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V
x
Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M
x
K

M
x
V

G
|
G

P

P

N

N

L
|
L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M
x
G

G

G

L

L

A
x
K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V
x
L

A

I

C

T

F
x
Y

L

L

K

K

E
x
K

binding mono-PEGylated sulfonatocalix[4]arene: A8 (≠ T30), K9 (≠ R31), A12 (= A34), R18 (≠ E40), Q21 (≠ V43), K32 (≠ M54), V33 (≠ M55), F87 (≠ Y109), G88 (≠ M110), K91 (≠ A113), Y102 (≠ F124), K105 (≠ E127)
binding heme c: C19 (= C41), C22 (= C44), H23 (= H45), G34 (= G56), L37 (= L59), I40 (≠ V62), S45 (= S67), Y51 (≠ F73), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), W64 (= W86), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), L99 (≠ V121)

5lftA Crystal structure of cytochromE C - bromo-trisulfonatocalix[4]arene complexes (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 5lftA

query
sites
5lftA

A

A

P

G

D
x
S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E

R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F

Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W

W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y

F

M

G

G

G

L
|
L

A
x
K

S

K

A

E

D
x
K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding Bromo-trisulfonatocalix[4]arene: S7 (≠ D29), L90 (= L112), K91 (≠ A113), K94 (≠ D116)
binding heme c: C19 (= C41), C22 (= C44), H23 (= H45), G34 (= G56), P35 (= P57), I40 (≠ V62), S45 (= S67), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107)

5kpfB Crystal structure of cytochromE C - phenyl-trisulfonatocalix[4]arene complex (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 5kpfB

query
sites
5kpfB

A

A

P

G

D
x
S

T
x
A

R
x
K

R
x
K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M
x
V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F

Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding Phenyl-trisulfonatocalix[4]arene: S7 (≠ D29), A8 (≠ T30), K9 (≠ R31), K10 (≠ R32)
binding heme c: R18 (≠ E40), C19 (= C41), C22 (= C44), H23 (= H45), V33 (≠ M55), G34 (= G56), P35 (= P57), I40 (≠ V62), S45 (= S67), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), W64 (= W86), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)

3tyiA Crystal structure of cytochromE C - p-sulfonatocalix[4]arene complexes (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 3tyiA

query
sites
3tyiA

A

A

P

G

D
x
S

T

A

R

K

R
x
K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F

Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S
x
K

A

E

D
x
K

D

D

R

R

Q
x
N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding protoporphyrin ix containing fe: R18 (≠ E40), C19 (= C41), C22 (= C44), H23 (= H45), G34 (= G56), P35 (= P57), I40 (≠ V62), S45 (= S67), G46 (= G68), Y53 (= Y75), T54 (≠ S76), N57 (≠ M79), W64 (= W86), M69 (≠ I91), Y72 (≠ L94), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)
binding 25,26,27,28-tetrahydroxypentacyclo[19.3.1.1~3,7~.1~9,13~.1~15,19~]octacosa-1(25),3(28),4,6,9(27),10,12,15(26),16,18,21,23-dodecaene-5,11,17,23-tetrasulfonic acid: S7 (≠ D29), K10 (≠ R32), K92 (≠ S114), K94 (≠ D116), N97 (≠ Q119)

2orlA Solution structure of the cytochromE C- para-aminophenol adduct (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 5:105/108 of 2orlA

query
sites
2orlA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E

R

C
|
C

S

L

V
x
Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G

G

P
|
P

N

N

L
|
L

F

H

G

G

V

I

Y
x
F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F

Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M

N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q
x
D

A
x
E

E

N

N

N

I

M

T

S

Q

E

L

Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K
|
K

E

K

binding 4-aminophenol: F41 (≠ Y63), D65 (≠ Q87), E66 (≠ A88), K104 (= K126)
binding heme c: C19 (= C41), Q21 (≠ V43), C22 (= C44), H23 (= H45), P35 (= P57), L37 (= L59), S45 (= S67), G46 (= G68), Y53 (= Y75), T54 (≠ S76), W64 (= W86), L73 (≠ I95), T83 (= T105), K84 (≠ Y106), M85 (= M107), F87 (≠ Y109)

7bbtC Structure of cytochromE C in complex with a p-benzyl-sulfonato- calix[8]arene-peg pseudorotaxane (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 4:104/107 of 7bbtC

query
sites
7bbtC

A

A

P

G

D

S

T

A

R

K

R
x
K

G

G

A

A

E
x
T

V

L

F

F

A
x
K

T
|
T

E
x
R

C
|
C

S

L

V
x
Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V
x
L

A

I

C

T

F

Y

L

L

K

K

E

K

binding heme c: R17 (≠ E40), C18 (= C41), C21 (= C44), H22 (= H45), G33 (= G56), P34 (= P57), I39 (≠ V62), S44 (= S67), G45 (= G68), Y50 (≠ F73), Y52 (= Y75), T53 (≠ S76), N56 (≠ M79), W63 (= W86), M68 (≠ I91), Y71 (≠ L94), L72 (≠ I95), T82 (= T105), K83 (≠ Y106), M84 (= M107), F86 (≠ Y109), L98 (≠ V121)
binding 4-[[49,50,51,52,53,54,55,56-octahydroxy-11,17,23,29,35,41,47-heptakis[(4-sulfonatophenyl)methyl]-5-nonacyclo[43.3.1.13,7.19,13.115,19.121,25.127,31.133,37.139,43]hexapentaconta-1(49),3,5,7(56),9,11,13(55),15,17,19(54),21,23,25(53),27,29,31(52),33,35,37(51),39,41,43(50),45,47-tetracosaenyl]methyl]benzenesulfonate: K9 (≠ R32), K9 (≠ R32), T12 (≠ E35), K15 (≠ A38), K15 (≠ A38), T16 (= T39), T16 (= T39), R17 (≠ E40), Q20 (≠ V43)

6suyA Yeast cytochromE C in complex with an octa-anionic calix[4]arene (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 4:104/107 of 6suyA

query
sites
6suyA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W

W

Q

D

A

E

E

N

N

N

I

M

T

S

Q

E

L

Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M
x
G

G
|
G

L

L

A
x
K

S
x
K

A

E

D

K

D

D

R

R

Q

N

A

D

V
x
L

A

I

C

T

F

Y

L

L

K

K

E

K

binding heme c: R17 (≠ E40), C18 (= C41), C21 (= C44), H22 (= H45), G33 (= G56), P34 (= P57), I39 (≠ V62), S44 (= S67), Y50 (≠ F73), Y52 (= Y75), T53 (≠ S76), N56 (≠ M79), L72 (≠ I95), T82 (= T105), K83 (≠ Y106), M84 (= M107), F86 (≠ Y109), L98 (≠ V121)
binding octa-anionic calix[4]arene: R17 (≠ E40), F86 (≠ Y109), G87 (≠ M110), G88 (= G111), K90 (≠ A113), K91 (≠ S114)

6s8yA Crystal structure of cytochromE C in complex with a sulfonated quinoline-derived foldamer (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 4:104/107 of 6s8yA

query
sites
6s8yA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T
x
P

G
x
H

M
x
K

M

V

G
|
G

P
|
P

N

N

L

L

F
x
H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I

L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding heme c: R17 (≠ E40), C18 (= C41), C21 (= C44), H22 (= H45), G33 (= G56), P34 (= P57), I39 (≠ V62), S44 (= S67), G45 (= G68), Y50 (≠ F73), Y52 (= Y75), T53 (≠ S76), N56 (≠ M79), W63 (= W86), M68 (≠ I91), Y71 (≠ L94), T82 (= T105), K83 (≠ Y106), M84 (= M107), F86 (≠ Y109)
binding 8-acetamido-2-[[2-[[2-[[2-[[2-[[2-[[2-[(2-carboxy-4-sulfonato-quinolin-8-yl)carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]-4-sulfonato-quinolin-8-yl]carbamoyl]quinoline-4-sulfonate: P29 (≠ T52), H30 (≠ G53), K31 (≠ M54)
binding zinc ion: H37 (≠ F60)

Sites not aligning to the query:

binding zinc ion: 107

5lycA CytochromE C in complex with phosphonato-calix[6]arene (see paper)
40% identity, 78% coverage: 27:127/129 of query aligns to 4:104/107 of 5lycA

query
sites
5lycA

A

A

P

G

D

S

T
x
A

R
x
K

R

K

G

G

A
|
A

E

T

V

L

F

F

A
x
K

T

T

E

R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M
x
V

G
|
G

P
|
P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G

G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q

D

A

A

M
x
N

R

I

D

K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q

N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F
x
Y

L

L

K

K

E
x
K

binding phosphonato-calix[6]arene: A7 (≠ T30), K8 (≠ R31), A11 (= A34), K15 (≠ A38), Y101 (≠ F124), K104 (≠ E127)
binding heme c: C18 (= C41), C21 (= C44), H22 (= H45), V32 (≠ M55), G33 (= G56), P34 (= P57), I39 (≠ V62), S44 (= S67), Y50 (≠ F73), Y52 (= Y75), T53 (≠ S76), N56 (≠ M79), W63 (= W86), M68 (≠ I91), Y71 (≠ L94), L72 (≠ I95), T82 (= T105), K83 (≠ Y106), M84 (= M107), F86 (≠ Y109)

5ncvA Crystal structure of cytochromE C in complex with p- methylphosphonatocalix[4]arene
40% identity, 78% coverage: 27:127/129 of query aligns to 1:101/104 of 5ncvA

query
sites
5ncvA

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E
x
R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G
|
G

P

P

N

N

L

L

F

H

G

G

V
x
I

Y

F

G

G

R

R

K

H

S
|
S

G
|
G

S

Q

L

A

A

E

G

G

F
x
Y

S

S

Y
|
Y

S
x
T

Q
x
D

A
|
A

M
x
N

R

I

D
x
K

K

K

D

N

V

V

D

L

W
|
W

Q

D

A

E

E

N

N

N

I
x
M

T

S

Q

E

L
x
Y

I
x
L

T

T

Q
x
N

P

P

Q

K

A

K

F

Y

V

I

P

P

G

G

T
|
T

Y
x
K

M
|
M

P

A

Y
x
F

M

G

G

G

L

L

A
x
K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

binding p-Methylphosphonatocalix[4]arene: D51 (≠ Q77), A52 (= A78), K55 (≠ D81), N71 (≠ Q97), K87 (≠ A113)
binding heme c: R14 (≠ E40), C15 (= C41), C18 (= C44), H19 (= H45), G30 (= G56), I36 (≠ V62), S41 (= S67), G42 (= G68), Y47 (≠ F73), Y49 (= Y75), T50 (≠ S76), N53 (≠ M79), W60 (= W86), M65 (≠ I91), Y68 (≠ L94), L69 (≠ I95), T79 (= T105), K80 (≠ Y106), M81 (= M107), F83 (≠ Y109)

P00044 Cytochrome c isoform 1; Iso-1-cytochrome c; Cytochrome c aerobic isoform from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
40% identity, 78% coverage: 27:127/129 of query aligns to 6:106/109 of P00044

query
sites
P00044

A

A

P

G

D

S

T

A

R

K

R

K

G

G

A

A

E

T

V

L

F

F

A

K

T

T

E

R

C
|
C

S

L

V

Q

C
|
C

H
|
H

A

T

V

V

T

E

K

K

G

G

T

G

T

P

G

H

M

K

M

V

G

G

P

P

N

N

L

L

F

H

G

G

V

I

Y

F

G

G

R

R

K

H

S

S

G

G

S

Q

L

A

A

E

G

G

F

Y

S

S

Y

Y

S

T

Q

D

A

A

M

N

R

I

D

K

K

K

D

N

V

V

D

L

W

W

Q

D

A

E

E

N

N

N

I

M

T

S

Q

E

L

Y

I

L

T

T

Q

N

P

P

Q
x
K

A
x
K

F

Y

V

I

P

P

G

G

T

T

Y

K

M
|
M

P

A

Y

F

M

G

G

G

L

L

A

K

S

K

A

E

D

K

D

D

R

R

Q

N

A

D

V

L

A

I

C

T

F

Y

L

L

K

K

E

K

C20 (= C41) binding covalent
C23 (= C44) binding covalent
H24 (= H45) binding axial binding residue
K78 (≠ Q99) modified: N6,N6,N6-trimethyllysine; by CTM1; mutation to R: Loss of methylation by CTM1.
K79 (≠ A100) modified: N6,N6,N6-trimethyllysine
M86 (= M107) binding axial binding residue

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>PfGW456L13_3738 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3738
MFTSLRVPVIGLLSFTLVQAVSAAECAPDTRRGAEVFATECSVCHAVTKGTTGMMGPNLF
GVYGRKSGSLAGFSYSQAMRDKDVDWQAENITQLITQPQAFVPGTYMPYMGLASADDRQA
VACFLKEQH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory