SitesBLAST
Comparing PfGW456L13_3817 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3817 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
56% identity, 99% coverage: 2:335/337 of query aligns to 2:337/337 of 1krhA
- active site: C306 (= C304)
- binding flavin-adenine dinucleotide: Y143 (= Y141), R155 (= R153), S156 (≠ A154), Y157 (= Y155), S158 (= S156), V171 (≠ L169), V172 (≠ I170), R173 (= R171), V175 (= V173), G178 (= G176), K179 (≠ L177), M180 (= M178), S181 (= S179), T219 (= T217), E332 (= E330), K333 (= K331), F334 (= F332), S335 (≠ A333), A336 (= A334), N337 (≠ S335)
- binding fe2/s2 (inorganic) cluster: M38 (≠ L38), D39 (= D39), C40 (= C40), R41 (= R41), G43 (= G43), E44 (≠ A44), C45 (= C45), G46 (= G46), C48 (= C48), C82 (= C81)
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 96% coverage: 15:336/337 of query aligns to 10:334/334 of P0DPQ8
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 96% coverage: 15:336/337 of query aligns to 9:333/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y141), R144 (= R153), Q145 (≠ A154), Y146 (= Y155), S147 (= S156), H161 (vs. gap), V162 (≠ I170), R163 (= R171), V165 (= V173), G168 (= G176), V169 (≠ L177), A170 (≠ M178), T171 (≠ S179), T214 (= T217), E327 (= E330), K328 (= K331), F329 (= F332), T330 (≠ A333), P331 (≠ A334), S333 (≠ A336)
- binding fe2/s2 (inorganic) cluster: N32 (≠ L38), S33 (≠ D39), C34 (= C40), N35 (≠ R41), G37 (= G43), C39 (= C45), G40 (= G46), C42 (= C48), C74 (= C81)
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
31% identity, 69% coverage: 105:337/337 of query aligns to 9:249/250 of 1tvcA
- active site: Y63 (= Y155), S64 (= S156), L215 (= L303)
- binding dihydroflavine-adenine dinucleotide: N24 (≠ S120), F49 (≠ Y141), R61 (= R153), S62 (≠ A154), Y63 (= Y155), S64 (= S156), L78 (= L169), I79 (= I170), R80 (= R171), V81 (≠ N172), L82 (≠ V173), R86 (≠ L177), F87 (≠ M178), V109 (≠ S200), G128 (= G218), N155 (≠ T245), E159 (≠ D249), S186 (vs. gap), G187 (vs. gap), E242 (= E330), F244 (= F332), L245 (≠ A333)
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
27% identity, 93% coverage: 6:317/337 of query aligns to 1:309/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C40), S38 (≠ D42), W57 (≠ G60), Y135 (= Y141), R146 (= R153), A147 (= A154), Y148 (= Y155), S149 (= S156), I162 (≠ L169), V163 (≠ I170), K164 (≠ R171), G169 (= G176), K170 (≠ L177), V171 (≠ M178), S172 (= S179), S209 (≠ T217)
- binding fe2/s2 (inorganic) cluster: Y34 (≠ L38), C36 (= C40), N37 (≠ R41), G39 (= G43), G40 (≠ A44), C41 (= C45), G42 (= G46), C44 (= C48), L74 (= L79), C76 (= C81)
Sites not aligning to the query:
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
27% identity, 92% coverage: 7:317/337 of query aligns to 2:309/326 of Q03304
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
29% identity, 91% coverage: 28:332/337 of query aligns to 28:333/334 of 7c3bC
- binding fe2/s2 (inorganic) cluster: Y38 (≠ L38), E39 (≠ D39), C40 (= C40), A41 (≠ R41), G43 (= G43), G44 (≠ A44), C45 (= C45), G46 (= G46), C48 (= C48), L79 (= L79), C81 (= C81)
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
29% identity, 91% coverage: 28:332/337 of query aligns to 27:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C40), S41 (≠ D42), W60 (≠ I64), F139 (≠ Y141), G149 (= G148), R152 (= R153), A153 (= A154), Y154 (= Y155), S155 (= S156), Y169 (≠ L169), I170 (= I170), K171 (≠ R171), G176 (= G176), R177 (≠ L177), F178 (≠ M178), S179 (= S179), A218 (≠ T217), S221 (≠ A220), A319 (≠ K331), F320 (= F332)
- binding fe2/s2 (inorganic) cluster: Y37 (≠ L38), E38 (≠ D39), C39 (= C40), A40 (≠ R41), G42 (= G43), G43 (≠ A44), C44 (= C45), G45 (= G46), C47 (= C48), C80 (= C81)
Sites not aligning to the query:
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
32% identity, 55% coverage: 139:323/337 of query aligns to 45:240/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R153), S62 (≠ A154), Y63 (= Y155), T64 (≠ S156), L79 (= L169), V80 (≠ I170), K81 (≠ R171), Y83 (≠ V173), T85 (≠ G175), G86 (= G176), N87 (≠ L177), V88 (≠ M178), S89 (= S179), T127 (= T217), A130 (= A220), P131 (= P221)
Sites not aligning to the query:
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
27% identity, 91% coverage: 28:332/337 of query aligns to 27:305/306 of 7c3bB
7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
26% identity, 54% coverage: 153:334/337 of query aligns to 83:280/280 of 7qu5A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (= A154), Y85 (= Y155), S86 (= S156), N100 (≠ L169), I101 (= I170), R102 (= R171), A104 (vs. gap), S105 (vs. gap), P106 (vs. gap), L112 (≠ V173), P113 (= P174), P114 (≠ G175), G115 (= G176), Q116 (≠ L177), M117 (= M178), S118 (= S179), A155 (≠ T217), F278 (= F332)
- binding gamma-Valerolactone: I103 (vs. gap), S105 (vs. gap), P108 (vs. gap), D196 (≠ E257), W207 (≠ F268), H208 (≠ S269), L209 (≠ A270)
Sites not aligning to the query:
7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
26% identity, 54% coverage: 153:333/337 of query aligns to 83:279/279 of 7qu3A
- binding flavin-adenine dinucleotide: R83 (= R153), A84 (= A154), Y85 (= Y155), S86 (= S156), N100 (≠ L169), I101 (= I170), R102 (= R171), A104 (vs. gap), S105 (vs. gap), P106 (vs. gap), L112 (≠ V173), P113 (= P174), P114 (≠ G175), G115 (= G176), Q116 (≠ L177), M117 (= M178), S118 (= S179), A155 (≠ T217), F278 (= F332)
- binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R171), G154 (= G216), G182 (= G243), A183 (≠ V244), S212 (≠ A273), F226 (≠ Y284), I227 (≠ V285), H228 (≠ T286), P252 (= P306), M254 (≠ P308), M255 (= M309)
Sites not aligning to the query:
8a1yF Na(+)-translocating NADH-quinone reductase subunit F
21% identity, 80% coverage: 66:334/337 of query aligns to 96:408/408 of 8a1yF
- binding flavin-adenine dinucleotide: R210 (= R153), A211 (= A154), Y212 (= Y155), S213 (= S156), N227 (= N172), V228 (= V173), R229 (vs. gap), A231 (vs. gap), T232 (vs. gap), P233 (vs. gap), V240 (vs. gap), P241 (= P174), P242 (≠ G175), G243 (= G176), Q244 (≠ L177), M245 (= M178), S246 (= S179), A283 (≠ T217), F406 (= F332)
- binding fe2/s2 (inorganic) cluster: C111 (= C81)
Sites not aligning to the query:
A5F5Y4 Na(+)-translocating NADH-quinone reductase subunit F; Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F; EC 7.2.1.1 from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395) (see paper)
21% identity, 80% coverage: 66:334/337 of query aligns to 96:408/408 of A5F5Y4
- C111 (= C81) binding ; mutation to A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- R210 (= R153) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- Y212 (= Y155) mutation to L: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
- S246 (= S179) mutation to A: Decreases flavin content, but does not affect the 2Fe-2S center. Exhibits very low NADH:quinone oxidoreductase activity.
Sites not aligning to the query:
- 70 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 76 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
- 79 binding ; C→A: Loss of the 2Fe-2S center, but does not affect flavin content. Exhibits very low NADH:quinone oxidoreductase activity.
4u9uA Crystal structure of nqrf fad-binding domain from vibrio cholerae (see paper)
25% identity, 47% coverage: 176:334/337 of query aligns to 114:279/279 of 4u9uA