SitesBLAST

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
30% identity, 46% coverage: 9:248/517 of query aligns to 6:243/353 of Q97UY8

query
sites
Q97UY8

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S142 (≠ G147) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G149) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E171) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs
33% identity, 39% coverage: 23:225/517 of query aligns to 23:224/229 of 7v8iD

query
sites
7v8iD

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binding phosphoaminophosphonic acid-adenylate ester: V23 (= V23), S43 (≠ N43), G44 (= G44), S45 (≠ A45), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49), Q93 (= Q90), R137 (≠ D138), H140 (≠ T141), E144 (= E145), L145 (= L146), S146 (≠ G147), G147 (≠ I148), G148 (= G149), E149 (≠ H150), N174 (≠ M175), H203 (= H203)
binding magnesium ion: S48 (= S48), Q93 (= Q90)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
28% identity, 41% coverage: 9:221/517 of query aligns to 1:221/232 of 1f3oA

query
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1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y19), A20 (≠ V23), S40 (≠ N43), G41 (= G44), S42 (≠ A45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
32% identity, 41% coverage: 9:221/517 of query aligns to 3:218/226 of 5xu1B

query
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5xu1B

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binding magnesium ion: G45 (= G46), K46 (= K47), S47 (= S48)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 42% coverage: 9:224/517 of query aligns to 1:215/240 of 4ymuJ

query
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4ymuJ

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binding adenosine-5'-triphosphate: F11 (≠ Y19), L14 (vs. gap), V16 (= V23), S36 (≠ N43), G37 (= G44), S38 (≠ A45), G39 (= G46), K40 (= K47), S41 (= S48), T42 (= T49), E51 (≠ V58), E162 (= E171), H194 (= H203)
binding magnesium ion: S41 (= S48), E162 (= E171)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
28% identity, 41% coverage: 9:221/517 of query aligns to 1:221/230 of 1l2tA

query
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1l2tA

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L

R

D

P

D

G

D

S

E

R

L

A

T

Q

K

A

I

E

R

A

R

L

D

G

K

I

I

R

G

M

F

V

V

M

F

Q

Q

E

Q

L

F

N

N

L

L

L

I

P

P

T

L

L

L

S

T

V

A

A

L

E

E

N

N

L

V

F

E

L

L

D

P

N

L

L

I

P

F

S

K

K

Y

G

R

G

G

W

A

I

M

S

S

R

G

K

E

Q

E

L

R

R

R

K

K

A

R

A

A

I

L

E

E

A

C

M

L

A

K

H

M

V

A

G

E

L

L

D

E

A

E

I

R

D
x
F

P

A

D

N

T
x
H

L

K

V

P

G

N

E
x
Q

L

L

G
x
S

I
x
G

G
|
G

H
x
Q

Q

Q

M

R

V

V

E

A

I

I

A

A

R

R

N

A

L

L

I

A

G

N

D

N

C

P

H

P

V

I

L

I

I

L

L

A

D

D

E

Q

P

P

T

T

A

G

M
x
A

L

L

T

D

A

S

R

K

E

T

V

G

E

E

M

K

L

I

F

M

E

Q

Q

L

I

L

T

K

R

K

L

L

-

N

Q

E

S

E

R

D

G

G

V

K

S

T

I

V

Y

V

I

V

S

T

H
|
H

R

D

L

I

E

-

E

N

L

V

A

A

R

R

V

F

A

G

Q

E

R

R

I

I

A

I

V

Y

L

L

R

K

D

D

G

G

binding adenosine-5'-triphosphate: Y11 (= Y19), M13 (≠ Q21), I18 (vs. gap), A20 (≠ V23), P39 (≠ E42), S40 (≠ N43), G41 (= G44), S42 (≠ A45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), F138 (≠ D138), H141 (≠ T141), Q145 (≠ E145), S147 (≠ G147), G148 (≠ I148), G149 (= G149), Q150 (≠ H150), A175 (≠ M175), H204 (= H203)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
31% identity, 40% coverage: 23:228/517 of query aligns to 23:226/650 of 5ws4A

query
sites
5ws4A

V
x
I

L

L

A

K

G

G

I

I

D

D

L

L

T

T

L

I

M

Y

R

E

G

G

E

E

V

L

L

V

A

A

L

I

T

V

G

G

E

Q

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

M

K

N

I

I

I

L

G

G

G

C

L

L

V

D

T

R

P

P

T

T

T

S

G

G

Q

S

M

Y

Q

K

Y

V

Q

N

G

G

Q

Q

D

E

-

T

-

G

-

K

Y

L

R

E

P

P

G

D

S

Q

R

L

A

A

Q

Q

A

L

E

R

A

R

L

E

G

Y

I

F

R

G

M

F

V

I

M

F

Q

Q

E

R

L

Y

N

H

L

L

P

G

T

D

L

L

S

S

V

A

A

E

E

G

N

N

L

V

F

-

L

-

D

-

N

E

L

V

P

P

S

A

K

V

G

Y

G

A

W

G

I

V

S

T

R

P

K

A

Q

D

L

R

R

K

K

Q

A

R

A

A

I

T

E

A

A

L

M

L

A

T

H

E

V

L

G

G

L

L

D

-

A

G

I

T

D

K

P

T

D

Q

T

N

L

R

V

P

G

S

E

Q

L

L

G

S

I

G

G

G

H

Q

Q

Q

M

R

V

V

E

S

I

I

A

A

R

R

N

A

L

L

I

M

G

N

D

G

C

G

H

D

V

V

L

I

I

L

L

A

D

D

E

E

P

P

T

T

A

G

M

A

L

L

T

D

A

S

R

H

E

S

V

G

E

V

M

E

L

V

F

M

E

R

Q

I

I

L

T

R

R

E

L

L

Q

N

S

A

R

A

G

G

V

H

S

T

I

I

Y

L

I

V

S

T

H

H

R

D

L

M

E

-

E

Q

L

V

A

A

R

K

V

N

A

A

Q

T

R

R

I

I

A

I

V

E

L

I

R

S

D

D

G

G

N

E

L

I

V

I

C

S

V

D

E

R

P

P

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: I23 (≠ V23), S43 (≠ N43), G44 (= G44), S45 (≠ A45), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 14

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
32% identity, 40% coverage: 23:228/517 of query aligns to 23:226/648 of P75831

query
sites
P75831

V

V

L

L

A

K

G

G

I

I

D

S

L

L

T

D

L

I

M

Y

R

A

G

G

E

E

V

M

L

V

A

A

L

I

T

V

G

G

E

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

S

M

K

N

I

I

I

L

G

G

G

C

L

L

V

D

T

K

P

A

T

T

T

S

G

G

Q

T

M

Y

Q

R

Y

V

Q

A

G

G

Q

Q

D

D

Y

V

R

A

P

T

-

L

-

D

-

A

G

D

S

A

R

L

A

A

Q

Q

A

L

E

R

A

R

L

E

G

H

I

F

R

G

M

F

V

I

M

F

Q

Q

E

R

L

Y

N

H

L

L

P

S

T

H

L

L

S

T

V

A

A

E

E

Q

N

N

L

V

F

-

L

-

D

-

N

E

L

V

P

P

S

A

K

V

G

Y

G

A

W

G

I

L

S

E

R

R

K

K

Q

Q

L

R

R

L

K

L

A

R

A

A

I

Q

E

E

A

L

M

L

A

Q

H

R

V

L

G

G

L

L

D

E

A

D

I

-

D

R

P

T

D

E

T

Y

L

Y

V

P

G

A

E

Q

L

L

G

S

I

G

G

G

H

Q

Q

Q

M

R

V

V

E

S

I

I

A

A

R

R

N

A

L

L

I

M

G

N

D

G

C

G

H

Q

V

V

L

I

I

L

L

A

D
|
D

E

E

P

P

T

T

A

G

M

A

L

L

T

D

A

S

R

H

E

S

V

G

E

E

M

E

L

V

F

M

E

A

Q

I

I

L

T

H

R

Q

L

L

Q

R

S

D

R

R

G

G

V

H

S

T

I

V

I

I

Y

I

I

V

S

T

H

H

R

D

L

-

E

P

E

Q

L

V

A

A

R

A

V

Q

A

A

Q

E

R

R

I

V

A

I

V

E

L

I

R

R

D

D

G

G

N

E

L

I

V

V

C

R

V

N

E

P

P

P

K47 (= K47) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
33% identity, 46% coverage: 8:243/517 of query aligns to 1:234/374 of 2awnB

query
sites
2awnB

A

A

V

S

L

V

S

Q

V

L

S

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

Q

E

P

V

V

V

L
x
V

A

S

G

K

-

D

I

I

D

N

L

L

T

D

L

I

M

H

R

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

Q

D

M

L

-

F

-

I

-

G

Q

E

Y

K

Q

R

G

M

Q

N

D

D

Y

T

R

P

P

P

G

A

S

E

R

R

A

-

Q

-

A

-

E

-

A

-

L

-

G

G

I

V

R

G

M

M

V

V

M

F

Q

Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

-

F

-

G

L

L

D

K

N

L

L

A

P

G

S

A

K

K

G

K

G

E

W

V

I

I

S

N

R

Q

K

R

Q

V

L

N

R

Q

K

V

A

A

A

E

I

V

E

L

A

Q

M

L

A

A

H

H

V

L

G

-

L

L

D

D

A

R

I

-

D

K

P

P

D

K

T

A

L

L

V

S

G

G

E

-

L

-

G

-

I

-

G

G

H

Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

H

S

V

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

M

N

L

L

-

D

T

A

A

A

R

L

E

R

V

V

E

Q

M

M

L

R

F

I

E

E

Q

-

I

I

T

S

R

R

L

L

Q

H

S

K

R

R

-

L

G

G

V

R

S

T

I

M

I

I

Y

Y

I

V

S

T

H

H

R

D

L

Q

E

V

E

E

L

A

A

M

R

T

V

L

A

A

Q

D

R

K

I

I

A

V

V

V

L

L

R

D

D

A

G

G

N

R

L

V

V

A

C

Q

V

V

-

G

E

K

P

P

M

L

A

E

-

L

-

Y

N

H

Y

Y

N

P

S

A

E

D

Q

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y19), V17 (≠ L24), S37 (≠ N43), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
33% identity, 46% coverage: 8:243/517 of query aligns to 1:234/372 of 2awoA

query
sites
2awoA

A

A

V

S

L

V

S

Q

V

L

S

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

Q

E

P

V

V

V

L
x
V

A

S

G

K

-

D

I

I

D

N

L

L

T

D

L

I

M

H

R

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

Q

D

M

L

-

F

-

I

-

G

Q

E

Y

K

Q

R

G

M

Q

N

D

D

Y

T

R

P

P

P

G

A

S

E

R

R

A

-

Q

-

A

-

E

-

A

-

L

-

G

G

I

V

R

G

M

M

V

V

M

F

Q

Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

-

F

-

G

L

L

D

K

N

L

L

A

P

G

S

A

K

K

G

K

G

E

W

V

I

I

S

N

R

Q

K

R

Q

V

L

N

R

Q

K

V

A

A

A

E

I

V

E

L

A

Q

M

L

A

A

H

H

V

L

G

-

L

L

D

D

A

R

I

-

D

K

P

P

D

K

T

A

L

L

V

S

G

G

E

-

L

-

G

-

I

-

G

G

H

Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

H

S

V

V

L

F

I

L

L

L

D
|
D

E

E

P

P

T

L

A

S

M

N

L

L

-

D

T

A

A

A

R

L

E

R

V

V

E

Q

M

M

L

R

F

I

E

E

Q

-

I

I

T

S

R

R

L

L

Q

H

S

K

R

R

-

L

G

G

V

R

S

T

I

M

I

I

Y

Y

I

V

S

T

H

H

R

D

L

Q

E

V

E

E

L

A

A

M

R

T

V

L

A

A

Q

D

R

K

I

I

A

V

V

V

L

L

R

D

D

A

G

G

N

R

L

V

V

A

C

Q

V

V

-

G

E

K

P

P

M

L

A

E

-

L

-

Y

N

H

Y

Y

N

P

S

A

E

D

Q

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ Y19), V17 (≠ L24), S37 (≠ N43), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49)
binding magnesium ion: S42 (= S48), D157 (= D170)

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
33% identity, 46% coverage: 8:243/517 of query aligns to 1:234/371 of 3puyA

query
sites
3puyA

A

A

V

S

L

V

S

Q

V

L

S

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

Q

E

P

V

V

V

L
x
V

A

S

G

K

-

D

I

I

D

N

L

L

T

D

L

I

M

H

R

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E
x
P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

S

L

K

R

I

M

I

I

G

A

G

G

L

L

V

E

T

T

P

I

T

T

T

S

G

G

Q

D

M

L

-

F

-

I

-

G

Q

E

Y

K

Q

R

G

M

Q

N

D

D

Y

T

R

P

P

P

G

A

S

E

R

R

A

-

Q

-

A

-

E

-

A

-

L

-

G

G

I

V

R

G

M

M

V

V

M

F

Q
|
Q

E

S

L

Y

N

A

L

L

L

Y

P

P

T

H

L

L

S

S

V

V

A

A

E

E

N

N

L

M

F

S

-

F

-

G

L

L

D

K

N

L

L

A

P

G

S

A

K

K

G

K

G

E

W

V

I

I

S

N

R

Q

K

R

Q

V

L

N

R

Q

K

V

A

A

A

E

I

V

E

L

A

Q

M

L

A

A

H

H

V

L

G

-

L

L

D

D

A
x
R

I

-

D

K

P

P

D

K

T
x
A

L

L

V
x
S

G
|
G

E

-

L

-

G

-

I

-

G
|
G

H
x
Q

Q

R

Q

Q

M

R

V

V

E

A

I

I

A

G

R

R

N

T

L

L

I

V

G

A

D

E

C

P

H

S

V

V

L

F

I

L

L

L

D
|
D

E
|
E

P

P

T

L

A

S

M

N

L

L

-

D

T

A

A

A

R

L

E

R

V

V

E

Q

M

M

L

R

F

I

E

E

Q

-

I

I

T

S

R

R

L

L

Q

H

S

K

R

R

-

L

G

G

V

R

S

T

I

M

I

I

Y

Y

I

V

S

T

H
|
H

R

D

L

Q

E

V

E

E

L

A

A

M

R

T

V

L

A

A

Q

D

R

K

I

I

A

V

V

V

L

L

R

D

D

A

G

G

N

R

L

V

V

A

C

Q

V

V

-

G

E

K

P

P

M

L

A

E

-

L

-

Y

N

H

Y

Y

N

P

S

A

E

D

Q

R

L

F

V

V

T

A

L

G

M

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y19), V17 (≠ L24), P36 (≠ E42), S37 (≠ N43), G38 (= G44), C39 (≠ A45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), Q81 (= Q90), R128 (≠ A136), A132 (≠ T141), S134 (≠ V143), G135 (= G144), G136 (= G149), Q137 (≠ H150), E158 (= E171), H191 (= H203)
binding magnesium ion: S42 (= S48), Q81 (= Q90), D157 (= D170)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
33% identity, 46% coverage: 8:243/517 of query aligns to 1:234/371 of 3puxA

query
sites
3puxA

A

A

V

S

L

V

S

Q

V

L

S

Q

G

N

I

V

G

T

K

K

T

A

Y
x
W

A

G

Q

E

P

V

V

V

L
x
V

A

S

G

K

-

D

I

I

D

N

L

L

T

D

L

I

M

H

R

E

G

G

E

E

V

F

L

V

A

V

L

F

T

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G