SitesBLAST

4ea8A X-ray crystal structure of perb from caulobacter crescentus in complex with coenzyme a and gdp-n-acetylperosamine at 1 angstrom resolution (see paper)
32% identity, 80% coverage: 30:205/221 of query aligns to 33:204/207 of 4ea8A

query
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4ea8A

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binding coenzyme a: H145 (≠ F146), F163 (= F164), G165 (= G166), V166 (≠ T167), A184 (= A185)
binding GDP-N-acetylperosamine: V35 (≠ L32), D36 (= D33), A37 (≠ N34), D50 (≠ T52), I68 (≠ T70), G69 (= G71)

Sites not aligning to the query:

4eaaA X-ray crystal structure of the h141n mutant of perosamine n- acetyltransferase from caulobacter crescentus in complex with coa and gdp-perosamine (see paper)
31% identity, 80% coverage: 30:205/221 of query aligns to 33:206/210 of 4eaaA

query
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4eaaA

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binding coenzyme a: P150 (= P149), F165 (= F164), V168 (≠ T167), A186 (= A185), I201 (≠ V200)
binding GDP-perosamine: V35 (≠ L32), D36 (= D33), A37 (≠ N34), D52 (≠ T52), I70 (≠ T70), G71 (= G71)

Sites not aligning to the query:

7txsA X-ray structure of the viob n-aetyltransferase from acinetobacter baumannii in the presence of a reaction intermediate (see paper)
32% identity, 81% coverage: 31:208/221 of query aligns to 30:206/209 of 7txsA

query
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7txsA

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binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3R)-4-({3-[(2-{[(1S)-1-{[(2R,3S,4S,5R,6R)-4,5-dihydroxy-6-{[(R)-hydroxy{[(R)-hydroxy{[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)oxolan-2-yl]methoxy}phosphoryl]oxy}phosphoryl]oxy}-2-methyloxan-3-yl]amino}ethyl]sulfanyl}ethyl)amino]-3-oxopropyl}amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate (non-preferred name): D32 (= D33), N33 (= N34), T34 (≠ D35), K37 (= K38), I66 (= I69), G67 (≠ T70), K74 (≠ Y77), I75 (≠ R78), P147 (= P149), G164 (= G166), A165 (≠ T167), M180 (≠ T182), G182 (= G184), M183 (≠ A185), V198 (= V200), L205 (≠ I207)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3R)-4-({3-[(2-{[(1S)-1-{[(2R,3S,4S,5R,6R)-4,5-dihydroxy-6-{[(R)-hydroxy{[(R)-hydroxy{[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)oxolan-2-yl]methoxy}phosphoryl]oxy}phosphoryl]oxy}-2-methyloxan-3-yl]amino}ethyl]sulfanyl}ethyl)amino]-3-oxopropyl}amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate (non-preferred name): 10, 11, 12

7txqA X-ray structure of the viob n-acetyltransferase from acinetobacter baumannii in the present of tdp and acetyl-coenzymea (see paper)
32% identity, 81% coverage: 31:208/221 of query aligns to 30:206/209 of 7txqA

query
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7txqA

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binding acetyl coenzyme *a: P147 (= P149), G164 (= G166), A165 (≠ T167), G182 (= G184), M183 (≠ A185), V198 (= V200), L205 (≠ I207)
binding thymidine-5'-diphosphate: D32 (= D33), N33 (= N34), T34 (≠ D35), K37 (= K38), I66 (= I69), G67 (≠ T70)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 10, 11, 12

7txpA X-ray structure of the viob n-acetyltransferase from acinetobacter baumannii in complex with tdp-4-amino-4,6-dideoxy-d-glucose (see paper)
32% identity, 81% coverage: 31:208/221 of query aligns to 30:206/209 of 7txpA

query
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7txpA

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binding dTDP-4-amino-4,6-dideoxyglucose: D32 (= D33), N33 (= N34), T34 (≠ D35), K37 (= K38), I66 (= I69), G67 (≠ T70), I75 (≠ R78)

Sites not aligning to the query:

binding dTDP-4-amino-4,6-dideoxyglucose: 10, 11, 12

4m99A Acetyltransferase domain of pglb from neisseria gonorrhoeae fa1090 in complex with acetyl coenzyme a (see paper)
29% identity, 81% coverage: 28:207/221 of query aligns to 29:206/206 of 4m99A

query
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4m99A

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I

L

binding acetyl coenzyme *a: D135 (≠ G136), P148 (= P149), G154 (= G155), G165 (= G166), T166 (= T167), R171 (≠ L172), Q172 (≠ P173), G183 (= G184), A184 (= A185), V189 (≠ T190), A199 (≠ V200), N201 (≠ S202)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
31% identity, 56% coverage: 93:215/221 of query aligns to 1:148/290 of 4mzuB

query
sites
4mzuB

F

M

I

I

H

H

P

K

G

L

I

A

D

D

L

V

T

Q

M

S

I

Q

T

N

M

I

G

G

V

D

G

N

T

T

Y

K

L

V

Q
x
W

E
x
Q

G

F

V

C

I

V

M

I

Q

L

A

A

Q

G

V

A

S

V

L

I

G

G

D

R

N

N

T

C

S

N

I

I

S
x
C

A
|
A

G

N

S

S

V

L

V

I

G

E

H

N

E

D

G

V

Q

V

I

I

G

G

H

D

S

N

V

V

F

T

M

I

A

K

P

S

G
|
G

V

V

C

Q

I

I

A

W

G

D

C

G

V

I

E

H

I

I

G

Q

D

D

G

D

T

V

F
|
F

I

I

G

G

-

P

-
x
N

-

V

-

T

-

F

-
x
T

-
x
N

-
x
D

-
x
K

-

Q

-
x
P

-

R

-

S

-

L

-

K

-

T

-

I

-

V

-

K

-

G

-

A

-

S

-

I

-
x
G

T
x
A

N

N

A

S

T

T

I

I

L

L

P

P

R

G

L

I

R

L

I

I

G

G

R

E

W

N

V

A

T

M

I

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

D

D

Y

N

A

A

V

I

V

V

V
x
I

G

G

S

N

P

P

A

G

R
|
R

T

I

I

T

K

G

T

Y

N

V

P

E

A

A

S

N

F

F

Q

E

binding coenzyme a: F72 (= F164), T80 (vs. gap), N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), P85 (vs. gap), G99 (vs. gap), A100 (≠ T167), G117 (= G184), A118 (= A185), T123 (= T190), K124 (= K191), I133 (≠ V200), R138 (= R205)
binding magnesium ion: G58 (= G150), N76 (vs. gap)
binding thymine: W20 (≠ Q112), Q21 (≠ E113), C38 (≠ S130), A39 (= A131)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

7l7yAAA Putative acetyl transferase protein (see paper)
31% identity, 92% coverage: 5:208/221 of query aligns to 16:217/217 of 7l7yAAA

query
sites
7l7yAAA

L

L

L

M

G

P

A

V

A

A

N

N

P

-

E

E

A

Q

V

L

R

R

M

I

I

L

H

-

T

-

L

-

Q

-

R

E

S

G

Q

D

P

T

D

D

V

S

Q

Q

F

I

A

V

F

F

L

I

D
|
D

N

-

D
|
D

P

A

A

L

K

D

Q

D

G

N

T

I

L

T

F

V

Y

N

G

G

V

Y

P

T

V

A

V

M

G

N

G

Y

T

T

D

-

K

K

V

F

A

K

E

S

L

I

K

K

G

N

P

D

D

D

T

-

R

K

F

F

V

V

N

L

L

I

I

A

T
x
I

G
x
A

T

N

T

S

A

S

L

I

R

R

Y

Q

R

K

T

I

T

A

C

D

E

K

I

L

V

V

E

K

A

D

G

G

G

I

T

S

L

L

G

W

N

T

F

-

I

V

H

Q

P

G

G

M

I

T

D

T

L

L

T

I

M

M

-

D

-

E

I

V

T

S

M

I

G

D

V

A

G

G

T

A

Y

A

L

L

Q

S

E

P

G

F

V

V

I

T

M

I

Q

A

A

A

Q

N

V

V

S

T

L

I

G

G

D

K

N

C

T

F

S

H

I

A

S

N

A

L

G

Y

S

S

V

Y

V

V

G

E

H

H

E

D

G

C

Q

I

I

I

G

G

H

D

S

Y

V

V

F

T

M

F

A

A

P

P

G

R

V

V

C

S

I

C

A

N

G

G

C

N

V

I

E

H

I

I

G

H

D

D

G

H

T

A

F
x
Y

I

I

G
|
G

T
|
T

N

G

A

A

T

V

I

I

L

K

-

Q

-

G

-

T

-

P

-

D

P

K

R

P

L

L

R

I

I

I

G

G

R

K

W

G

V

A

T

I

I

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

Y

G

A

A

V

V

V
x
I

G

G

S

N

P

P

A

A

R

R

T

L

I

L

K

N

binding acetyl coenzyme *a: Y168 (≠ F164), G170 (= G166), T171 (= T167), G193 (= G184), M194 (≠ A185), I209 (≠ V200)
binding uridine-5'-diphosphate: D39 (= D33), D40 (= D35), I73 (≠ T70), A74 (≠ G71)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 10, 12

7l82AAA Putative acetyl transferase protein (see paper)
31% identity, 92% coverage: 5:207/221 of query aligns to 16:216/216 of 7l82AAA

query
sites
7l82AAA

L

L

L

M

G

P

A

V

A

A

N

N

P

-

E

E

A

Q

V

L

R

R

M

I

I

L

H

-

T

-

L

-

Q

-

R

E

S

G

Q

D

P

T

D

D

V

S

Q

Q

F

I

A

V

F

F

L

I

D
|
D

N

-

D
|
D

P

A

A

L

K

D

Q

D

G

N

T

I

L

T

F

V

Y

N

G

G

V

Y

P

T

V

A

V

M

G

N

G

Y

T

T

D

-

K

K

V

F

A

K

E

S

L

I

K

K

G

N

P

D

D

D

T

-

R

K

F

F

V

V

N

L

L

I

I

A

T
x
I

G
x
A

T

N

T

S

A

S

L

I

R

R

Y

Q

R

K

T

I

T

A

C

D

E

K

I

L

V

V

E

K

A

D

G

G

G

I

T

S

L

L

G

W

N

T

F

-

I

V

H

Q

P

G

G

M

I

T

D

T

L

L

T

I

M

M

-

D

-

E

I

V

T

S

M

I

G

D

V

A

G

G

T

A

Y

A

L

L

Q

S

E

P

G

F

V

V

I

T

M

I

Q

A

A

A

Q

N

V

V

S

T

L

I

G

G

D

K

N

C

T

F

S

H

I

A

S

N

A

L

G

Y

S

S

V

Y

V

V

G

E

H

H

E

D

G

C

Q

I

I

I

G

G

H

D

S

Y

V

V

F

T

M

F

A

A

P

P

G

R

V

V

C

S

I

C

A

N

G

G

C

N

V

I

E

H

I

I

G

H

D

D

G

H

T

A

F
x
Y

I

I

G

G

T
|
T

N

G

A

A

T

V

I

I

L

K

-

Q

-

G

-

T

-

P

-

D

P

K

R

P

L

L

R

I

I

I

G

G

R

K

W

G

V

A

T

I

I

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

Y

G

A

A

V

V

V
x
I

G

G

S

N

P

P

A

A

R

R

T

L

I

L

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: D39 (= D33), D40 (= D35), I73 (≠ T70), A74 (≠ G71), Y168 (≠ F164), T171 (= T167), G193 (= G184), M194 (≠ A185), I209 (≠ V200)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 10, 12

7l81AAA Putative acetyl transferase protein (see paper)
31% identity, 92% coverage: 5:207/221 of query aligns to 16:216/216 of 7l81AAA

query
sites
7l81AAA

L

L

L

M

G

P

A

V

A

A

N

N

P

-

E

E

A

Q

V

L

R

R

M

I

I

L

H

-

T

-

L

-

Q

-

R

E

S

G

Q

D

P

T

D

D

V

S

Q

Q

F

I

A

V

F

F

L

I

D
|
D

N

-

D
|
D

P

A

A

L

K

D

Q

D

G

N

T

I

L

T

F

V

Y

N

G

G

V

Y

P

T

V

A

V

M

G

N

G

Y

T

T

D

-

K

K

V

F

A

K

E

S

L

I

K

K

G

N

P

D

D

D

T

-

R

K

F

F

V

V

N

L

L

I

I

A

T
x
I

G
x
A

T

N

T

S

A

S

L

I

R

R

Y

Q

R

K

T

I

T

A

C

D

E

K

I

L

V

V

E

K

A

D

G

G

G

I

T

S

L

L

G

W

N

T

F

-

I

V

H

Q

P

G

G

M

I

T

D

T

L

L

T

I

M

M

-

D

-

E

I

V

T

S

M

I

G

D

V

A

G

G

T

A

Y

A

L

L

Q

S

E

P

G

F

V

V

I

T

M

I

Q

A

A

A

Q

N

V

V

S

T

L

I

G

G

D

K

N

C

T

F

S

H

I

A

S

N

A

L

G

Y

S

S

V

Y

V

V

G

E

H

H

E

D

G

C

Q

I

I

I

G

G

H

D

S

Y

V

V

F

T

M

F

A
|
A

P

P

G

R

V

V

C

S

I

C

A

N

G

G

C

N

V

I

E

H

I

I

G

H

D

D

G

H

T

A

F
x
Y

I

I

G

G

T
|
T

N

G

A

A

T

V

I

I

L

K

-

Q

-

G

-

T

-

P

-

D

P

K

R

P

L

L

R

I

I

I

G

G

R

K

W

G

V

A

T

I

I

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

Y

G

A

A

V

V

V
x
I

G

G

S

N

P

P

A

A

R

R

T

L

I

L

binding coenzyme a: A152 (= A148), Y168 (≠ F164), T171 (= T167), G193 (= G184), M194 (≠ A185), I209 (≠ V200)
binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: D39 (= D33), D40 (= D35), I73 (≠ T70), A74 (≠ G71)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 10, 12

7l7zAAA Putative acetyl transferase protein (see paper)
31% identity, 92% coverage: 5:207/221 of query aligns to 16:216/216 of 7l7zAAA

query
sites
7l7zAAA

L

L

L

M

G

P

A

V

A

A

N

N

P

-

E

E

A

Q

V

L

R

R

M

I

I

L

H

-

T

-

L

-

Q

-

R

E

S

G

Q

D

P

T

D

D

V

S

Q

Q

F

I

A

V

F

F

L

I

D
|
D

N

-

D
|
D

P

A

A

L

K

D

Q

D

G

N

T

I

L

T

F

V

Y

N

G

G

V

Y

P

T

V

A

V

M

G

N

G

Y

T

T

D

-

K

K

V

F

A

K

E

S

L

I

K

K

G

N

P

D

D

D

T

-

R

K

F

F

V

V

N

L

L

I

I

A

T
x
I

G
x
A

T

N

T

S

A

S

L

I

R

R

Y

Q

R

K

T

I

T

A

C

D

E

K

I

L

V

V

E

K

A

D

G

G

G

I

T

S

L

L

G

W

N

T

F

-

I

V

H

Q

P

G

G

M

I

T

D

T

L

L

T

I

M

M

-

D

-

E

I

V

T

S

M

I

G

D

V

A

G

G

T

A

Y

A

L

L

Q

S

E

P

G

F

V

V

I

T

M

I

Q

A

A

A

Q

N

V

V

S

T

L

I

G

G

D

K

N

C

T

F

S

H

I

A

S

N

A

L

G

Y

S

S

V

Y

V

V

G

E

H

H

E

D

G

C

Q

I

I

I

G

G

H

D

S

Y

V

V

F

T

M

F

A

A

P

P

G

R

V

V

C

S

I

C

A

N

G

G

C

N

V

I

E

H

I

I

G

H

D

D

G

H

T

A

F
x
Y

I

I

G

G

T
|
T

N

G

A

A

T

V

I

I

L

K

-

Q

-

G

-

T

-

P

-

D

P

K

R

P

L

L

R

I

I

I

G

G

R

K

W

G

V

A

T

I

I

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

Y

G

A

A

V

V

V
x
I

G

G

S

N

P

P

A

A

R

R

T

L

I

L

binding coenzyme a: Y168 (≠ F164), T171 (= T167), G193 (= G184), M194 (≠ A185), I209 (≠ V200)
binding UDP-di-N-acetyl-alpha-bacillosamine: D39 (= D33), D40 (= D35), I73 (≠ T70), A74 (≠ G71)

Sites not aligning to the query:

binding UDP-di-N-acetyl-alpha-bacillosamine: 10, 12

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
32% identity, 51% coverage: 93:205/221 of query aligns to 1:139/294 of 4mzuF

query
sites
4mzuF

F

M

I

I

H

H

P

K

G

L

I

A

D

D

L

V

T

Q

M

S

I

Q

T

N

M

I

G

G

V

D

G

N

T

T

Y

K

L

V

Q
x
W

E
x
Q

G

F

V

C

I

V

M

I

Q

L

A

A

Q

G

V

A

S

V

L

I

G

G

D

R

N

N

T

C

S

N

I

I

S
x
C

A

A

G

N

S

S

V

L

V

I

G

E

H

N

E

D

G

V

Q

V

I

I

G

G

H

D

S

N

V

V

F

T

M

I

A

K

P

S

G

G

V

V

C

Q

I

I

A

W

G

D

C

G

V

I

E

H

I

I

G

Q

D

D

G

D

T

V

F

F

I

I

G

G

-

P

-

N

-

V

-

T

-

F

-

T

-
x
N

-
x
D

-
x
K

-

Q

-

P

-

R

-

S

-

K

-

I

-

K

-

T

-

I

-

V

-

K

-

G

-

A

-

S

-

I

-
x
G

T
x
A

N

N

A

S

T

T

I

I

L
|
L

P

P

R

G

L

I

R

L

I

I

G

G

R

E

W

N

V

A

T

M

I

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

D

D

Y

N

A

A

V

I

V

V

V
x
I

G

G

S
x
N

P

P

A

G

R
|
R

binding coenzyme a: N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), G100 (vs. gap), A101 (≠ T167), L106 (= L172), G118 (= G184), A119 (= A185), T124 (= T190), K125 (= K191), I134 (≠ V200), N136 (≠ S202), R139 (= R205)
binding thymine: W20 (≠ Q112), Q21 (≠ E113), C38 (≠ S130)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 189, 203, 205, 270

3bssA Pgld from campylobacter jejuni, nctc 11168, with native substrate (see paper)
27% identity, 62% coverage: 71:207/221 of query aligns to 55:194/194 of 3bssA

query
sites
3bssA

G
|
G

T

N

A

E

L
x
I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L

I

T

S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q

N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S

N

A

T

G

S

S

S

V

V

V

I

G

E

H

H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F

H

M

V

A

S

P

V

G

G

V

A

C

K

I

C

A

A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F

F

I

L

G

G

T

I

N

N

A

S

T

C

I

V

L

L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G

G

A

G

G

G

A

A

V

T

V

L

T

V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V

V

G

G

S

V

P

P

A

A

R

K

T

R

I

M

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: G55 (= G71), I59 (≠ L75)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 12, 14, 34, 35, 36, 54

2vheA Pgld-coa complex: an acetyl transferase from campylobacter jejuni (see paper)
27% identity, 62% coverage: 71:207/221 of query aligns to 55:194/194 of 2vheA

query
sites
2vheA

G

G

T

N

A

E

L

I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L

I

T

S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q

N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S

N

A

T

G

S

S

S

V

V

V

I

G

E

H

H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F

H

M

V

A
x
S

P
x
V

G

G

V

A

C

K

I

C

A

A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F
|
F

I

L

G
|
G

T
x
I

N

N

A

S

T

C

I

V

L

L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G
|
G

A
x
G

G

G

A

A

V

T

V

L

T

V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V
|
V

G

G

S

V

P

P

A

A

R

K

T

R

I
x
M

binding coenzyme a: S135 (≠ A148), V136 (≠ P149), F151 (= F164), G153 (= G166), I154 (≠ T167), G171 (= G184), G172 (≠ A185), V187 (= V200), M194 (≠ I207)

3bsyA Pgld from campylobacter jejuni, nctc 11168, in complex with acetyl coenzyme a (see paper)
27% identity, 62% coverage: 71:207/221 of query aligns to 54:193/193 of 3bsyA

query
sites
3bsyA

G

G

T

N

A

E

L

I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L

I

T

S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q

N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S

N

A

T

G

S

S

S

V

V

V

I

G

E

H

H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F
x
H

M

V

A
x
S

P
x
V

G

G

V

A

C

K

I

C

A
|
A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F
|
F

I

L

G
|
G

T
x
I

N

N

A

S

T

C

I

V

L
|
L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G
|
G

A
x
G

G

G

A

A

V

T

V

L

T
x
V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V
|
V

G

G

S

V

P
|
P

A

A

R

K

T

R

I

M

binding acetyl coenzyme *a: H132 (≠ F146), S134 (≠ A148), V135 (≠ P149), A140 (= A154), F150 (= F164), G152 (= G166), I153 (≠ T167), L158 (= L172), G170 (= G184), G171 (≠ A185), V176 (≠ T190), V186 (= V200), P189 (= P203)

Q0P9D1 UDP-N-acetylbacillosamine N-acetyltransferase; Protein glycosylation D; UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine N-acetyltransferase; EC 2.3.1.203 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see 2 papers)
27% identity, 62% coverage: 71:207/221 of query aligns to 56:195/195 of Q0P9D1

query
sites
Q0P9D1

G

G

T

N

A

E

L

I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L

I

T

S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q

N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S
x
N

A

T

G

S

S

S

V

V

V

I

G
x
E

H
|
H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F
x
H

M

V

A

S

P

V

G

G

V

A

C

K

I

C

A

A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F

F

I

L

G

G

T
x
I

N

N

A

S

T

C

I

V

L

L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G

G

A
x
G

G

G

A

A

V

T

V

L

T

V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V

V

G

G

S

V

P

P

A

A

R

K

T

R

I

M

N118 (≠ S130) mutation to A: Reduction in catalytic activity.
E124 (≠ G136) mutation to A: Reduction in catalytic activity.
H125 (= H137) active site, Proton acceptor; mutation to A: Strong reduction in catalytic activity.
H134 (≠ F146) binding ; mutation to A: Slight reduction in catalytic activity.
I155 (≠ T167) binding
G173 (≠ A185) binding

Sites not aligning to the query:

15 H→A: Induces a higher KM and approximate 3-fold decrease in the turnover number.

5t2yA Crystal structure of c. Jejuni pgld in complex with 5-methyl-4- (methylamino)-2-phenethylthieno[2,3-d]pyrimidine-6-carboxylic acid (see paper)
27% identity, 62% coverage: 71:207/221 of query aligns to 53:192/192 of 5t2yA

query
sites
5t2yA

G

G

T

N

A

E

L

I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L

I

T

S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q

N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S

N

A

T

G

S

S

S

V

V

V

I

G

E

H

H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F

H

M

V

A
x
S

P

V

G

G

V

A

C

K

I

C

A

A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F
|
F

I

L

G
|
G

T
x
I

N

N

A

S

T

C

I

V

L

L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G

G

A
x
G

G

G

A

A

V

T

V

L

T

V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V
|
V

G

G

S

V

P

P

A

A

R

K

T

R

I

M

binding 5-methyl-4-(methylamino)-2-(2-phenylethyl)thieno[2,3-d]pyrimidine-6-carboxylic acid: S133 (≠ A148), F149 (= F164), G151 (= G166), I152 (≠ T167), G170 (≠ A185), V185 (= V200)

5tyhA Pgld from campylobacter jejuni nctc 11168 in complex with 5-(2- furanyl)-1h-pyrazole-3-carboxylic acid (see paper)
27% identity, 62% coverage: 71:207/221 of query aligns to 46:185/185 of 5tyhA

query
sites
5tyhA

G

G

T

N

A

E

L

I

R

R

Y

K

R

K

T

I

T

Y

C

Q

E

K

I

I

V

S

E

E

A

N

G

G

G

F

T

K

L

I

G

V

N

N

F

L

I

I

H

H

P

K

G

S

I

A

D

L

L
x
I

T
x
S

-

P

-

S

-

A

M

I

I

V

T

E

M

E

G

N

V

A

G

G

T

I

Y

L

L

I

Q

M

E

P

G

Y

V

V

I

V

M

I

Q
x
N

A

A

Q

K

V

A

S

K

L

I

G

E

D

K

N

G

T

V

S

I

I

L

S
x
N

A

T

G

S

S

S

V

V

V

I

G

E

H

H

E

E

G

C

Q

V

I

I

G

G

H

E

S

F

V

S

F

H

M

V

A
x
S

P
x
V

G

G

V

A

C

K

I

C

A

A

G

G

C

N

V

V

E

K

I

I

G

G

D

K

G

N

T

C

F
|
F

I

L

G
|
G

T
x
I

N

N

A

S

T

C

I

V

L

L

P

P

R

N

L

L

R

S

I

L

G

A

R

D

W

D

V

S

T

I

I

L

G

G

A
x
G

G

G

A

A

V

T

V

L

T

V

K

K

D

N

V

Q

P

D

D

E

Y

K

A

G

V

V

V

F

V

V

G

G

S

V

P

P

A

A

R

K

T

R

I

M

binding 3-(furan-2-yl)-1H-pyrazole-5-carboxylic acid: I75 (≠ L100), S76 (≠ T101), N96 (≠ Q118), N108 (≠ S130), S126 (≠ A148), V127 (≠ P149), F142 (= F164), G144 (= G166), I145 (≠ T167), G163 (≠ A185)

Query Sequence

>PfGW456L13_4374 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4374
MKVYLLGAANPEAVRMIHTLQRSQPDVQFAFLDNDPAKQGTLFYGVPVVGGTDKVAELKG
PDTRFVNLITGTTALRYRTTCEIVEAGGTLGNFIHPGIDLTMITMGVGTYLQEGVIMQAQ
VSLGDNTSISAGSVVGHEGQIGHSVFMAPGVCIAGCVEIGDGTFIGTNATILPRLRIGRW
VTIGAGAVVTKDVPDYAVVVGSPARTIKTNPASFQDGRVFN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory