SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_4635 Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
49% identity, 88% coverage: 28:257/260 of query aligns to 3:233/235 of 5owfA

query
sites
5owfA

L

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binding glutamine: Y11 (= Y36), F49 (= F74), S66 (= S91), S67 (= S92), L68 (≠ M93), S69 (≠ T94), R74 (= R99), K114 (≠ E139), S117 (≠ T142), T118 (≠ I143), Q119 (= Q144), D158 (= D183)

1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
49% identity, 89% coverage: 26:257/260 of query aligns to 4:236/238 of 1lstA

query
sites
1lstA

K

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D

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binding lysine: D11 (= D33), Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), L117 (≠ E139), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
49% identity, 89% coverage: 26:257/260 of query aligns to 4:236/238 of 1lahE

query
sites
1lahE

K

Q

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binding L-ornithine: D11 (= D33), Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
49% identity, 89% coverage: 26:257/260 of query aligns to 4:236/238 of 1lagE

query
sites
1lagE

K

Q

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-

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-

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D

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A

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N

D

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D

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Y

binding histidine: Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), L117 (≠ E139), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
49% identity, 89% coverage: 26:257/260 of query aligns to 4:236/238 of 1lafE

query
sites
1lafE

K

Q

E

T

L

V

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R

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D
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D

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A

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K

K

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Y

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K

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K

D

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D

K

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T

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K

K

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D

N

D

T

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E

L

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K

A

A

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F

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D

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L

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D

D

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G

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Y

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A

K

K

H

Y

F

F

G

-

D

D

L

F

N

N

L

V

Y

Y

binding arginine: D11 (= D33), Y14 (= Y36), F52 (= F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), L117 (≠ E139), S120 (≠ T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
43% identity, 90% coverage: 26:259/260 of query aligns to 26:260/260 of P02910

query
sites
P02910

K

Q

E

K

L

I

R

R

F

I

G

G

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T

D

D

P

P

S

T

Y

Y

A

A

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P

F

F

E
|
E

S

S

K
|
K

A

N

A

A

D

Q

G

G

S
x
E

L

L

V

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

E

I

L

C
|
C

A

K

E

R

L

I

K

N

V

T

K

Q

C
|
C

K

T

W

F

V

V

E

E

S

N

D

P

F

L

D

D

G

A

M

L

I

I

P

P

G

S

L

L

K

K

A

A

N

K

K

K

F

I

D

D

G

A

V

I

I

M

S

S

M

L

T

S

V

I

T

T

P

E

A

K

R

R

E

Q

K

Q

V

E

I

I

D

A

F

F

S

T

S

D

E

K

L

L

F

Y

S

A

G

A

P

D

T

S

A

R

Y

L

V

V

F

V

K

A

K

K

G

N

S

S

G

D

L

I

T

Q

E

P

D

T

V

V

A

A

S

S

L

L

K

K

G

G

K

K

T

R

V

V

G

G

Y

V

E

L

Q

Q

G

G

T

T

I

T

Q

Q

E

E

A

T

Y

F

A

G

K

N

A

E

V

H

L

W

D

A

K

P

A

K

G

G

V

I

K

E

T

I

Q

V

A

S

Y

Y

Q

Q

N

G

Q

Q

D

D

Q

N

V

I

Y

Y

S

S

D
|
D

L

L

T

T

S

A

G

G

R
|
R

L

I

D
|
D

A

A

I

F

Q

Q

D

D

M

E

L

V

Q

A

A

A

E

S

L

E

G

G

F

F

L

L

K

K

S

Q

P

P

K

V

G

G

E

K

G

D

Y

Y

E

K

V

F

S

G

K

G

P

P

V

A

-

V

-

K

D

D

S

E

E

K

L

L

L

F

P

G

A

V

K

G

T

T

A

G

V

M

G

G

I

L

K

R

K

K

G

E

N

D

T

N

E

E

L

L

K

R

A

E

L

A

L

L

D

N

K

K

G

A

I

F

K

A

A

E

L

M

H

R

D

A

D

D

G

G

T

T

Y

Y

A

E

T

K

I

L

Q

A

K

K

H

Y

F

F

G

-

D

D

L

F

N

D

L

V

Y

Y

S

G

G

G

E40 (= E40) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
K42 (= K42) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
E47 (≠ S47) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
C60 (= C60) modified: Disulfide link with 67
C67 (= C67) modified: Disulfide link with 60
D171 (= D171) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
R176 (= R176) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
D178 (= D178) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.

Sites not aligning to the query:

1:22 signal peptide

1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
43% identity, 90% coverage: 26:259/260 of query aligns to 4:238/238 of 1hslA

query
sites
1hslA

K

Q

E

K

L

I

R

R

F

I

G

G

V

T

D

D

P

P

S

T

Y
|
Y

A

A

P

P

F

F

E

E

S

S

K

K

A

N

A

A

D

Q

G

G

S

E

L

L

V

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

E

I

L

C

C

A

K

E

R

L

I

K

N

V

T

K

Q

C

C

K

T

W

F

V

V

E

E

S

N

D

P

F
x
L

D

D

G

A

M

L

I

I

P

P

G

S

L

L

K

K

A

A

N

K

K

K

F

I

D

D

G

A

V

I

I

M

S
|
S

M
x
L

T
x
S

V

I

T

T

P

E

A

K

R
|
R

E

Q

K

Q

V

E

I

I

D

A

F

F

S

T

S

D

E

K

L

L

F

Y

S

A

G

A

P

D

T

S

A

R

Y

L

V

V

F

V

K

A

K

K

G

N

S

S

G

D

L

I

T

Q

E

P

D

T

V

V

A

A

S

S

L

L

K

K

G

G

K

K

T

R

V

V

G

G

Y

V

E
x
L

Q

Q

G

G

T
|
T

I
x
T

Q
|
Q

E

E

A

T

Y

F

A

G

K

N

A

E

V

H

L

W

D

A

K

P

A

K

G

G

V

I

K

E

T

I

Q

V

A

S

Y

Y

Q

Q

N

G

Q

Q

D

D

Q

N

V

I

Y

Y

S

S

D

D

L

L

T

T

S

A

G

G

R

R

L

I

D

D

A

A

I

F

Q

Q

D
|
D

M

E

L

V

Q

A

A

A

E

S

L

E

G

G

F

F

L

L

K

K

S

Q

P

P

K

V

G

G

E

K

G

D

Y

Y

E

K

V

F

S

G

K

G

P

P

V

A

-

V

-

K

D

D

S

E

E

K

L

L

L

F

P

G

A

V

K

G

T

T

A

G

V

M

G

G

I

L

K

R

K

K

G

E

N

D

T

N

E

E

L

L

K

R

A

E

L

A

L

L

D

N

K

K

G

A

I

F

K

A

A

E

L

M

H

R

D

A

D

D

G

G

T

T

Y

Y

A

E

T

K

I

L

Q

A

K

K

H

Y

F

F

G

-

D

D

L

F

N

D

L

V

Y

Y

S

G

G

G

binding histidine: Y14 (= Y36), L52 (≠ F74), S69 (= S91), S70 (= S92), L71 (≠ M93), S72 (≠ T94), R77 (= R99), L117 (≠ E139), T120 (= T142), T121 (≠ I143), Q122 (= Q144), D161 (= D183)

4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
38% identity, 87% coverage: 28:252/260 of query aligns to 6:226/226 of 4zv1A

query
sites
4zv1A

L

L

R

R

F

V

G

G

V

T

D
x
E

P

A

S

T

Y
x
F

A

P

P

P

F

F

E

G

S

F

K

K

A

D

A

E

D

N

G

G

S

K

L

L

V

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

A

I

I

C

A

A

K

E

K

L

L

K

G

V

V

K

K

C

V

K

E

W

F

V

K

E

P

S

M

D

D

F
|
F

D

D

G

G

M

I

I

I

P

P

G

A

L

L

K

Q

A

S

N

G

K

K

F

I

D

D

G

V

V

V

I

I

S
x
A

S
x
G

M
|
M

T
|
T

V

I

T

T

P

E

A

E

R
|
R

E

K

K

K

V

Q

I

V

D

D

F

F

S

S

S

D

E

P

L

Y

F

F

S

E

G

A

P

G

T

Q

A

A

Y

I

V

V

F

V

K

K

G

G

S

N

G

D

L

S

T

I

E

K

D

S

V

L

A

E

S

D

L

L

K

K

G

G

K

K

T

K

V

V

G

G

Y

V

E
x
Q

Q

L

G

G

T
x
S

I
x
T

Q

S

E

E

A

Q

Y

H

A

V

K

K

A

K

V

V

L

A

D

K

K

D

A

A

G

G

V

V

K

K

T

V

Q

K

A

K

Y

F

Q

D

N

N

Q

F

D

S

Q

E

V

A

Y

F

S

Q

D

E

L

L

T

K

S

S

G

G

R

R

L

V

D

D

A

A

A

V

I

V

Q

T

D
|
D

M

N

L

A

Q

V

A

A

E

-

L

L

G

A

F

Y

L

V

K

K

S

Q

P

N

K

P

G

N

E

A

G

G

Y

V

E

K

V

I

S

-

K

-

P

-

V

V

D

G

S

E

E

T

L

F

L

S

P

G

A

E

K

P

T

Y

A

G

V

I

G

A

I

V

K

R

K

K

G

G

N

N

T

S

E

E

L

L

K

L

A

E

L

K

L

I

D

N

K

K

G

A

I

L

K

E

A

E

L

M

H

K

D

K

D

D

G

G

T

T

Y

Y

A

D

T

K

I

I

Q

Y

K

E

K

K

H

W

F

F

G

G

binding arginine: E11 (≠ D33), F14 (≠ Y36), F52 (= F74), A69 (≠ S91), G70 (≠ S92), M71 (= M93), T72 (= T94), R77 (= R99), Q117 (≠ E139), S120 (≠ T142), T121 (≠ I143), D161 (= D183)

4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
38% identity, 87% coverage: 28:253/260 of query aligns to 6:225/225 of 4zv2A

query
sites
4zv2A

L

L

R

R

F

V

G

G

V

T

D
x
E

P

A

S

T

Y
x
F

A

P

P

P

F

F

E

G

S

F

K

K

A

D

A

E

D

N

G

G

S

K

L

L

V

V

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

A

I

I

C

A

A

K

E

K

L

L

K

G

V

V

K

K

C

V

K

E

W

F

V

K

E

P

S

M

D

D

F
|
F

D

D

G

G

M

I

I

I

P

P

G

A

L

L

K

Q

A

S

N

G

K

K

F

I

D

D

G

V

V

V

I

I

S
x
A

S
x
G

M
|
M

T
|
T

V

I

T

T

P

E

A

E

R
|
R

E

K

K

K

V

Q

I

V

D

D

F

F

S

S

S

D

E

P

L

Y

F

F

S

E

G

A

P

G

T

Q

A

A

Y

I

V

V

F

V

K

K

G

G

S

N

G

D

L

S

T

I

E

K

D

S

V

L

A

E

S

D

L

L

K

K

G

G

K

K

T

K

V

V

G

G

Y

V

E

Q

Q

L

G

G

T
x
S

I
x
T

Q

S

E

E

A

Q

Y

H

A

V

K

K

A

K

V

V

L

A

D

-

K

-

A

A

G

G

V

V

K

K

T

V

Q

K

A

K

Y

F

Q

D

N

N

Q

F

D

S

Q

E

V

A

Y

F

S

Q

D

E

L

L

T

K

S

S

G

G

R

R

L

V

D

D

A

A

A

V

I

V

Q

T

D
|
D

M

N

L

A

Q

V

A

A

E

-

L

L

G

A

F

Y

L

V

K

K

S

Q

P

N

K

P

G

N

E

A

G

G

Y

V

E

K

V

I

S

-

K

-

P

-

V

V

D

G

S

E

E

T

L

F

L

S

P

G

A

E

K

P

T

Y

A

G

V

I

G

A

I

V

K

R

K

K

G

G

N

N

T

S

E

E

L

L

K

L

A

E

L

K

L

I

D

N

K

K

G

A

I

L

K

E

A

E

L

M

H

K

D

K

D

D

G

G

T

T

Y

Y

A

D

T

K

I

I

Q

Y

K

E

K

K

H

W

F

F

G

G

D

E

binding glutamine: E11 (≠ D33), F14 (≠ Y36), F52 (= F74), A69 (≠ S91), G70 (≠ S92), M71 (= M93), T72 (= T94), R77 (= R99), S120 (≠ T142), T121 (≠ I143), D159 (= D183)

5itoA Structure of the periplasmic binding protein m117n-noct from a. Tumefaciens in complex with octopine (see paper)
33% identity, 88% coverage: 24:252/260 of query aligns to 1:250/255 of 5itoA

query
sites
5itoA

E

D

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F
x
Y

E
x
N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G

T

P

P

T

N

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A

A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K

G

T

V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding octopine: E10 (≠ D33), Y13 (= Y36), Y16 (≠ F39), N17 (≠ E40), W51 (≠ F74), A68 (≠ S91), A69 (≠ S92), M70 (= M93), G71 (≠ T94), R76 (= R99), Q139 (≠ E139), T142 (= T142), S143 (≠ I143), H144 (≠ Q144), S181 (≠ E188)

5ovzA High resolution structure of the pbp noct in complex with nopaline (see paper)
33% identity, 88% coverage: 24:252/260 of query aligns to 1:250/259 of 5ovzA

query
sites
5ovzA

E

D

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F
x
Y

E
x
N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G
x
T

P

P

T
x
M

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A

A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K
x
G

T
x
V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding N-[(1S)-4-carbamimidamido-1-carboxybutyl]-D-glutamic acid: E10 (≠ D33), Y13 (= Y36), Y16 (≠ F39), N17 (≠ E40), W51 (≠ F74), A68 (≠ S91), A69 (≠ S92), M70 (= M93), G71 (≠ T94), R76 (= R99), T89 (≠ G112), M91 (≠ T114), Q139 (≠ E139), T142 (= T142), S143 (≠ I143), H144 (≠ Q144), S181 (≠ E188), G212 (≠ K214), V213 (≠ T215)

5otaA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with octopinic acid (see paper)
33% identity, 88% coverage: 25:252/260 of query aligns to 1:249/254 of 5otaA

query
sites
5otaA

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F
x
Y

E

N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S

A

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G

T

P

P

T
x
M

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A
|
A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K

G

T

V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding (2~{S})-5-azanyl-2-[[(2~{R})-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]pentanoic acid: E9 (≠ D33), Y12 (= Y36), Y15 (≠ F39), W50 (≠ F74), A68 (≠ S92), M69 (= M93), G70 (≠ T94), R75 (= R99), M90 (≠ T114), Q138 (≠ E139), T141 (= T142), S142 (≠ I143), H143 (≠ Q144), A179 (= A187), S180 (≠ E188)

5ot9A Structure of the periplasmic binding protein (pbp) noct from a.Tumefaciens c58 in complex with histopine. (see paper)
33% identity, 88% coverage: 25:252/260 of query aligns to 1:249/254 of 5ot9A

query
sites
5ot9A

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F
x
Y

E

N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G

T

P

P

T
x
M

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A

A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K

G

T

V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding Histopine: E9 (≠ D33), Y12 (= Y36), Y15 (≠ F39), W50 (≠ F74), A67 (≠ S91), A68 (≠ S92), M69 (= M93), G70 (≠ T94), R75 (= R99), M90 (≠ T114), Q138 (≠ E139), T141 (= T142), S142 (≠ I143), H143 (≠ Q144), S180 (≠ E188)

4powA Structure of the pbp noct in complex with pyronopaline (see paper)
33% identity, 88% coverage: 25:252/260 of query aligns to 1:249/254 of 4powA

query
sites
4powA

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F

Y

E
x
N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G
x
T

P

P

T
x
M

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A

A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K

G

T

V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding 1-[(1S)-4-carbamimidamido-1-carboxybutyl]-5-oxo-D-proline: E9 (≠ D33), Y12 (= Y36), N16 (≠ E40), W50 (≠ F74), A67 (≠ S91), A68 (≠ S92), M69 (= M93), G70 (≠ T94), R75 (= R99), T88 (≠ G112), M90 (≠ T114), Q138 (≠ E139), T141 (= T142), S142 (≠ I143), H143 (≠ Q144), S180 (≠ E188)

5otcA Structure of the periplasmic binding protein (pbp) noct from agrobacterium tumefaciens c58 in complex with noroctopinic acid. (see paper)
33% identity, 88% coverage: 25:252/260 of query aligns to 1:249/256 of 5otcA

query
sites
5otcA

Y

Y

K

K

E

S

L

I

R

T

F

I

G

A

V

T

D
x
E

P

G

S

S

Y
|
Y

A

A

P

P

F
x
Y

E

N

S

F

K

K

A

D

A

A

D

G

G

G

S

K

L

L

V

I

G

G

F

F

D

D

I

I

D

D

L

L

G

G

N

N

A

D

I

L

C

C

A

K

E

R

L

M

K

N

V

I

K

E

C

C

K

K

W

F

V

V

E

E

S

Q

D

A

F
x
W

D

D

G

G

M

I

I

I

P

P

G

S

L

L

K

T

A

A

N

G

K

R

F

Y

D

D

G

A

V

I

I

M

S

A

S
x
A

M
|
M

T
x
G

V

I

T

Q

P

P

A

A

R
|
R

E

E

K

K

V

V

I

I

D

A

F

F

S

S

S

R

E

P

L

Y

F

L

S

L

G

T

P

P

T

M

A

T

Y

F

V

L

F

T

K

T

K

A

G

D

S

S

G

P

L

L

T

L

E

K

D

T

V

Q

A

V

S

A

L

I

-

E

-

N

-

L

-

P

-

L

-

D

-

N

-

I

-

T

-

P

-

E

-

Q

-

K

-

A

-

E

-

L

-

D

-

K

-

F

-

T

-

K

-

I

-

F

K

E

G

G

K

V

T

K

V

F

G

G

Y

V

E
x
Q

Q

A

G

G

T
|
T

I
x
S

Q
x
H

E

E

A

A

Y

F

A

M

K

K

A

Q

V

M

L

M

D

-

K

-

A

P

G

S

V

V

K

Q

T

I

Q

S

A

T

Y

Y

Q

D

N

T

Q

I

D

D

Q

N

V

V

Y

V

S

M

D

D

L

L

T

K

S

A

G

G

R

R

L

I

D

D

A

A

A

S

I

L

Q

-

D

-

M

-

L

-

Q

-

A

A

E
x
S

L

V

G

S

F

F

L

L

K

K

S

P

-

L

-

T

-

D

-

K

P

P

K

D

G

N

E

K

G

D

Y

L

E

K

V

M

S

F

K

G

P

P

-

R

V

M

D

T

S

G

E

G

L

L

L

F

P

G

A

K

K

G

T

V

A

G

V

V

G

G

I

I

K

R

K

K

G

E

N

D

T

A

E

D

L

L

K

K

A

A

L

L

L

F

D

D

K

K

G

A

I

I

K

D

A

A

L

A

H

I

D

A

D

D

G

G

T

T

Y

V

A

Q

T

K

I

L

Q

S

K

Q

H

W

F

F

G

G

binding (2~{S})-5-azanyl-2-(2-hydroxy-2-oxoethylamino)pentanoic acid: E9 (≠ D33), Y12 (= Y36), Y15 (≠ F39), W50 (≠ F74), A68 (≠ S92), M69 (= M93), G70 (≠ T94), R75 (= R99), Q138 (≠ E139), T141 (= T142), S142 (≠ I143), H143 (≠ Q144), S180 (≠ E188)

2y7iA Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
32% identity, 87% coverage: 26:251/260 of query aligns to 6:227/228 of 2y7iA

query
sites
2y7iA

K

R

E

T

L

L

R
x
H

F

F

G

G

V

T

D
x
S

P

A

S

T

Y
|
Y

A

A

P

P

F

Y

E
|
E

S

F

K

V

A
x
D

A

A

D
|
D

G

N

S

K

L

I

V

V

G

G

F

F

D

D

I

I

D

D

L

V

G

A

N

N

A

A

I

V

C

C

A

K

E

E

L

M

K

Q

V

A

K

E

C

C

K

S

W

F

V

T

E

N

S

Q

D

S

F
|
F

D
|
D

G

S

M

L

I

I

P

P

G

S

L

L

K

R

A

F

N

K

K

K

F

F

D
|
D

G

A

V

V

I

I

S
x
A

S
x
G

M
|
M

T
x
D

V

M

T

T

P

P

A

K

R
|
R

E

E

K

Q

V

Q

I

V

D

S

F

F

S

S

S

Q

E

P

L

Y

F

Y

S

E

G

G

P

L

T

S

A

A

Y

V

V

V

F

V

K

T

K

R

G

-

S

K

G

G

L

A

T

Y

E

H

D

T

V

F

A

A

S

D

L

L

K

K

G

G

K

K

T

K

V

V

G

G

Y

L

E
|
E

Q

N

G

G

T
|
T

I
x
T

Q
x
H

E

Q

A

R

Y

Y

A

L

K

Q

A

D

V

-

L

-

D

K

K

Q

A

Q

G

A

V

I

K

T

T

P

Q

V

A

A

Y

Y

Q
x
D

N

S

Q

Y

D

L

Q

N

V

A

Y

F

S

T

D

D

L

L

T

K

S

N

G

N

R

R

L

L

D

E

A

G

A

V

I

F

Q

G

D
|
D

M

V

L

-

Q

-

A

A

E

A

L

I

G

G

-

K

F

W

L

L

K

K

S

N

P

N

K

P

G

D

E

Y

G

A

Y

I

E

M

V

D

S

E

K

R

P

A

V

S

D

D

S

P

E

D

L

Y

P

G

A

K

K

G

T

L

A

G

V

I

G

A

I

V

K

R

K

K

G

D

N

N

T

D

E

A

L

L

K

L

A

Q

L

E

L

I

D

N

K

A

G

A

I

L

K

D

A

K

L

V

H

K

D

A

D

S

G

P

T

E

Y

Y

A

A

T

Q

I

M

Q

Q

K

E

K

K

H

W

F

F

binding arginine: S13 (≠ D33), Y16 (= Y36), E20 (= E40), F54 (= F74), A71 (≠ S91), G72 (≠ S92), M73 (= M93), D74 (≠ T94), R79 (= R99), E118 (= E139), T121 (= T142), T122 (≠ I143), H123 (≠ Q144), D160 (= D183)
binding zinc ion: H9 (≠ R29), D23 (≠ A43), D25 (= D45), D55 (= D75), D67 (= D87), D140 (≠ Q163)

5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
35% identity, 86% coverage: 28:251/260 of query aligns to 12:228/229 of 5t0wA

query
sites
5t0wA

L

L

R

R

F

V

G

G

V

T

D
|
D

P

A

S

D

Y
|
Y

A

K

P

P

F

F

E

S

S

F

K

K

A

D

A

K

D

N

G

G

S

Q

L

Y

V

T

G

G

F

F

D

D

I

I

D

D

L

L

G

A

N

K

A

A

I

L

C

A

A

K

E

E

L

L

K

G

V

V

K

K

C

V

K

E

W

F

V

V

E

P

S

T

D

T

F
x
W

D

D

G

G

M

I

I

I

P

P

G

A

L

L

K

Q

A

T

N

G

K

K

F

F

D

D

G

I

V

V

I

M

S
|
S

S
x
G

M
|
M

T
|
T

V

I

T

T

P

P

A

E

R
|
R

E

K

K

K

V

K

I

V

D

D

F

F

S

S

S

D

E

P

-

Y

L

M

F

T

S

A

G

G

P

Q

T

T

A

I

Y

L

V

V

F

K

K

K

K

D

G

N

S

A

G

D

L

K

T

I

E

K

D

S

V

F

A

E

S

D

L

L

K

N

G

K

K

P

-

D

-

V

T

K

V

V

G

A

Y

V

E
x
Q

Q

L

G

G

T
|
T

I
x
T

Q

S

E

E

A

Q

Y

A

A

A

K

K

A

E

V

F

L

L

D

P

K

K

A

A

G

K

V

I

K

R

T

T

Q

-

A

-

Y

F

Q

E

N

N

Q

N

D

A

Q

E

V

A

Y

F

S

Q

D

E

L

V

T

V

S

S

G

G

R

R

L

A

D

D

A

A

A

M

I

V

Q

T

D
|
D

M

-

L

-

Q

-

A

-

E

-

L

-

G

-

F

-

L

-

K

-

S

S

P

P

K

V

G

A

E

A

G

Y

Y

Y

E

A

-

K

-

L

-

A

-

V

V

V

S

D

K

E

P

P

V

F

D

T

S

H

E

E

L

P

L

L

P

-

A

-

K

-

T

-

A

G

V

F

G

A

I

I

K

R

K

K

G

G

N

D

T

P

E

E

L

L

K

L

A

N

L

W

L

V

D

N

K

N

G

W

I

L

K

K

A

Q

L

M

H

K

D

K

D

D

G

G

T

T

Y

Y

A

D

T

K

I

L

Q

Y

K

E

K

K

H

W

F

F

binding arginine: D17 (= D33), Y20 (= Y36), W58 (≠ F74), S75 (= S91), G76 (≠ S92), M77 (= M93), T78 (= T94), R83 (= R99), Q126 (≠ E139), T129 (= T142), T130 (≠ I143), D168 (= D183)

3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
32% identity, 89% coverage: 23:253/260 of query aligns to 14:236/241 of 3vvfA

query
sites
3vvfA

K

K

E

R

Y

S

K

G

E

E

L

I

R

R

F

I

G

G

V

T

D
x
E

P

G

S

A

Y
x
F

A

P

P

P

F

F

E
x
N

S

Y

K

F

A

D

A

E

D

R

G

N

S

Q

L

L

V

T

G

G

F

F

D

E

I

V

D

D

L

L

G

G

N

N

A

A

I

I

C

A

A

E

E

R

L

L

K

G

V

L

K

K

C

P

K

R

W

W

V

I

E

A

S

Q

D

S

F
|
F

D

D

G

T

M

L

I

L

P

I

G

Q

L

L

K

N

A

Q

N

G

K

R

F

F

D

D

G

F

V

V

I

I

S
x
A

S
|
S

M
x
H

T
x
G

V

I

T

T

P

E

A

E

R
|
R

E

A

K

R

V

A

I

V

D

D

F

F

S

T

S

N

E

P

L

H

F

Y

S

C

G

T

P

G

T

G

A

V

Y

I

V

V

F

S

K

R

K

K

G

G

S

G

G

P

L

R

T

T

E

-

D

-

V

A

A

K

S

D

L

L

K

Q

G

G

K

K

T

V

V

V

G

G

Y

V

E
x
Q

Q

V

G

G

T
|
T

I

-

Q

-

E

-

A
x
T

Y
|
Y

A

M

K

E

A

A

V

A

L

Q

D

K

K

I

A

P

G

G

V

I

K

K

-

E

T

V

Q

R

A

T

Y

Y

Q

Q

N

R

Q

D

D

P

Q

D

V

A

Y

L

S

Q

D

D

L

L

T

L

S

A

G

G

R

R

L

I

D

D

A

T

A

W

I

I

Q

T

D

D

M

R

L

F

Q

V

A

A

E

K

L

-

G

-

F

-

L

-

K

E

S

A

P

I

K

K

G

E

E

R

G

K

Y

L

E

E

V

N

S

T

K

L

P

Q

V

V

D

G

S

E

E

L

L

V

L

F

P

Q

A
x
E

K

R

T

V

A

A

V

M

G

A

I

V

K

A

K

K

G

G

N

N

T

K

E

S

L

L

K

L

A

D

L

A

L

L

D

N

K

R

G

A

I

L

K

A

A

E

L

L

H

M

D

Q

D

D

G

G

T

T

Y

Y

A

A

T

R

I

I