SitesBLAST

Comparing PfGW456L13_5043 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_5043 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 18 hits to proteins with known functional sites

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
44% identity, 90% coverage: 30:459/480 of query aligns to 6:429/430 of P0AA76

• Y29 (= Y53) binding
• D31 (= D55) mutation to N: Loss of galactonate transport activity.
• R32 (= R56) binding
• Y64 (= Y88) binding
• E118 (= E148) mutation to Q: Loss of galactonate transport activity.
• W358 (= W387) binding

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
44% identity, 88% coverage: 38:458/480 of query aligns to 3:409/409 of 6e9nA

• binding D-gluconic acid: F111 (= F152), N359 (= N407)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
41% identity, 89% coverage: 33:458/480 of query aligns to 1:393/393 of 6e9oA

• binding D-galactonic acid: Y21 (= Y53), F114 (= F152), Q141 (= Q179), Q228 (= Q278), S320 (≠ A384), N343 (= N407)

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 89% coverage: 13:438/480 of query aligns to 28:481/582 of Q9JI12

• R88 (= R56) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
• H128 (≠ F81) mutation to A: Greatly lowers L-glutamate transport.
• R184 (= R141) mutation R->A,E,K: Greatly lowers L-glutamate transport.
• E191 (= E148) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
• R322 (≠ I281) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
26% identity, 81% coverage: 48:438/480 of query aligns to 35:423/452 of 7t3nA

• binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y88), Y137 (≠ F152), Y165 (= Y180), R264 (≠ I281), F268 (≠ T285), Y269 (= Y286)
• binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (= E167), G163 (≠ A178)

Sites not aligning to the query:

• binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
26% identity, 85% coverage: 57:463/480 of query aligns to 88:489/495 of Q8BN82

• H183 (≠ A156) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

• 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
25% identity, 85% coverage: 57:463/480 of query aligns to 88:489/495 of Q9NRA2

• K136 (= K105) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
• H183 (≠ A156) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
• LL 198:199 (≠ AS 171:172) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
• I-----L 266:267 (≠ ISENGGM 238:244) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
• SSLRN 268:272 (≠ VDMDQ 245:249) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
• G328 (= G308) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
• P334 (= P314) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
• G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

• 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
• 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
25% identity, 86% coverage: 57:469/480 of query aligns to 88:495/495 of Q5Q0U0

• K136 (= K105) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
• R168 (= R141) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
• E171 (≠ V144) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
• G172 (= G145) mutation to C: Decreases protein levels and alters subcellular localization.
• E175 (= E148) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
• G176 (≠ A149) mutation to C: Decreases protein levels and alters subcellular localization.
• F179 (= F152) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
• P180 (= P153) mutation to C: Decreases protein levels and alters subcellular localization.
• H183 (≠ A156) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
• W186 (≠ V159) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
• SSLKN 268:272 (≠ VDMDQ 245:249) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
• P334 (= P314) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
• G371 (= G350) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

• 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
• 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
26% identity, 78% coverage: 62:437/480 of query aligns to 68:443/493 of Q03567

• N69 (≠ G63) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
26% identity, 75% coverage: 78:438/480 of query aligns to 111:464/497 of Q9Y2C5

• A372 (≠ I348) to T: in dbSNP:rs11754288

Sites not aligning to the query:

• 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
• 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
• 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
27% identity, 74% coverage: 38:392/480 of query aligns to 113:448/605 of Q9GQQ0

• E217 (= E148) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
23% identity, 92% coverage: 38:478/480 of query aligns to 20:447/452 of Q5EXK5