SitesBLAST
Comparing PfGW456L13_7 ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
32% identity, 91% coverage: 14:489/521 of query aligns to 5:474/501 of P04983
- K43 (= K52) mutation to R: Loss of transport.
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 43% coverage: 12:233/521 of query aligns to 1:223/241 of 4u00A
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 44% coverage: 14:244/521 of query aligns to 4:232/369 of P19566
- L86 (= L99) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (≠ T178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
Sites not aligning to the query:
- 306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 43% coverage: 12:236/521 of query aligns to 16:238/378 of P69874
- C26 (≠ R22) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (≠ Y23) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ I41) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (≠ A50) mutation to T: Loss of ATPase activity and transport.
- L60 (≠ M56) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ W72) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (≠ Y134) mutation to M: Loss of ATPase activity and transport.
- D172 (= D171) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 3:231/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 3:231/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y23), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (= Q95), R128 (= R142), A132 (≠ S146), S134 (= S148), G136 (= G150), Q137 (≠ E151), E158 (= E172), H191 (= H204)
- binding magnesium ion: S42 (= S53), Q81 (= Q95)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 3:231/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y23), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R142), S134 (= S148), Q137 (≠ E151)
- binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S148), G136 (= G150), H191 (= H204)
- binding magnesium ion: S42 (= S53), Q81 (= Q95)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 3:231/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R142), A132 (≠ S146), S134 (= S148), Q137 (≠ E151)
- binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S148), G135 (≠ I149), G136 (= G150), E158 (= E172), H191 (= H204)
- binding magnesium ion: S42 (= S53), Q81 (= Q95)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 3:231/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R142), A132 (≠ S146), S134 (= S148), Q137 (≠ E151)
- binding magnesium ion: S42 (= S53), Q81 (= Q95)
6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
31% identity, 45% coverage: 14:245/521 of query aligns to 3:236/238 of 6s8nB
6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
31% identity, 45% coverage: 14:245/521 of query aligns to 3:236/238 of 6s8gA
- binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y23), R15 (≠ C26), N37 (= N48), G40 (= G51), K41 (= K52), T42 (≠ S53), T43 (= T54), Q84 (= Q95), S136 (= S146), S138 (= S148), E141 (= E151)
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
30% identity, 44% coverage: 14:244/521 of query aligns to 1:229/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y23), S35 (≠ N48), G36 (= G49), C37 (≠ A50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (= R142), A130 (≠ S146), S132 (= S148), G134 (= G150), Q135 (≠ E151)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
30% identity, 44% coverage: 14:244/521 of query aligns to 4:232/371 of P68187
- A85 (≠ S98) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ T119) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ I127) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (= V130) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ R132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (≠ T137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (= R240) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
3c4jA Abc protein artp in complex with atp-gamma-s
28% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
28% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
28% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
28% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 2oljA
6mbnA Lptb e163q in complex with atp (see paper)
31% identity, 45% coverage: 14:249/521 of query aligns to 4:241/241 of 6mbnA
6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
31% identity, 44% coverage: 14:244/521 of query aligns to 3:235/235 of 6mhzA
- binding adp orthovanadate: Y12 (= Y23), N37 (= N48), G38 (= G49), G40 (= G51), K41 (= K52), T42 (≠ S53), T43 (= T54), Q84 (= Q95), S136 (= S146), S138 (= S148), G139 (≠ I149), G140 (= G150), E162 (= E172), G166 (≠ V176), H194 (= H204)
6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
29% identity, 41% coverage: 14:225/521 of query aligns to 4:218/229 of 6z67B
Query Sequence
>PfGW456L13_7 ABC transporter ATP-binding protein
MPNHAPIPDPVPRLQLRRITKRYPGCLANDAIDLTIAPGEIHALLGENGAGKSTLMKIIY
GVTHADSGEVIWQGQRVSLRNPAQARGLGIGMVFQHFSLFETLSVAQNIALAMGAAAGTP
KQLEPKIREVSRRYGMTLEPERLVHSLSIGERQRVEIIRCLMQDIRLLILDEPTSVLTPQ
EADDLFVTLRRLAAEGCSILFISHKLGEVRALCHSATVLRGGRVAGHCVPAECSDQQLAR
LMVGEAAELIADYPKVTGGDACLDVRGLSWHNPDPFGCSLANIDLEVRRGEIVGIAGVAG
NGQDELLALLSGEALLPRNASATIRFGKEPVAHLRPDARRQLGLAFVPAERLGHGAVPEL
SLADNALLTAFQHGLVSNGLIQRGKVEALAEEIIRRFGVKTPDSQAPARSLSGGNLQKFI
LGREILQQPRLLVAAHPTWGVDVGAAATIHRALIALRDAGAAILVISEDLDELFQISDRL
GALCGGRLSALHATVDTRLSDVGGWMAGQFGAPQSLASATR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory