SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_736 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_736 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

Q9I6J1 Putrescine-binding periplasmic protein SpuD from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
59% identity, 100% coverage: 1:365/366 of query aligns to 2:366/367 of Q9I6J1

query
sites
Q9I6J1

M

M

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K

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SD 36:37 (≠ FD 34:35) binding putrescine
C173 (= C172) modified: Disulfide link with 236
C236 (= C235) modified: Disulfide link with 173
D244 (≠ S243) binding putrescine
F273 (≠ W272) mutation to W: Significant decrease in affinity for putrescine (to KD=0.75 uM).
D275 (= D274) binding putrescine

3ttmA Crystal structure of spud in complex with putrescine (see paper)
61% identity, 93% coverage: 24:365/366 of query aligns to 1:341/341 of 3ttmA

query
sites
3ttmA

E

D

E

N

R

K

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L

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G

binding 1,4-diaminobutane: W10 (= W33), S11 (≠ F34), D12 (= D35), Y13 (= Y36), Y216 (= Y240), D219 (≠ S243), F248 (≠ W272), D250 (= D274), F286 (≠ Y310)

P31133 Putrescine-binding periplasmic protein PotF from Escherichia coli (strain K12) (see 3 papers)
55% identity, 99% coverage: 4:365/366 of query aligns to 9:369/370 of P31133

query
sites
P31133

L
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K
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G

S38 (≠ F34) binding putrescine
C175 (= C172) modified: Disulfide link with 239
C239 (= C235) modified: Disulfide link with 175
D247 (≠ S243) binding putrescine
D278 (= D274) binding putrescine

Sites not aligning to the query:

1:26 signal peptide

Q9I6J0 Spermidine-binding periplasmic protein SpuE from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
53% identity, 99% coverage: 2:365/366 of query aligns to 3:364/365 of Q9I6J0

query
sites
Q9I6J0

N

H

R

S

L

I

K

G

R

K

F

T

I

L

A

L

P

V

A

A

L

A

C

L

A

A

T

T

L

A

L

I

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A

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G

A

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A

A

E

E

E

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L

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R

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Y

Y

N

N

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W

F
x
T

D

D

Y

Y

I

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T

A

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A

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L

L

E

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D

D

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F

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-

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K

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D

L

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Y

Y

D

D

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N

N

E

E

A

T

L

L

E

E

A

G

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K

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L

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S

G

G

N

H

S

S

G

G

Y

Y

D

D

V

I

V

V

P

P

S

S

N

N

V

N

F

F

L

L

A

G

K

K

Q

Q

I

I

E

Q

A

A

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A

F

F

Q

Q

P

K

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

N

W

W

N

K

H

N

L

L

D

D

P

P

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A

L

L

M

L

K

K

L

Q

I

L

E

E

A

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N

S

D

D

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P

G

G

N

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F

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A

A

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Y

W

G

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N

L

G

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Y

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N

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A

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K

K

K

L

L

K

A

Q

K

C

C

G

G

V

V

A

A

L

F

L

M

D

D

S

S

P

G

S

D

E
|
E

I

M

L

L

P

P

L

A

A

A

L

L

Q

N

H

Y

L

L

G

G

L

L

D

D

P

P

N

N

S

T

K

H

K

D

P

P

A

K

D

D

Y

Y

D

K

K

K

A

A

E

E

A

E

L

V

L

L

M

T

K

K

I

V

R

R

P

P

Y

Y

I

V

T

S

Y

Y

F

F

H

H

S

S

K

K

Y

Y

M

I

A

S

D

D

I

L

A

A

N

N

G

G

D

N

I

I

C

C

V

V

A

A

V

F

G

G

Y

Y

S

S

G

G

S
x
D

F

V

S

F

Q

Q

A

A

N

A

R

R

A

A

K

E

E

E

A

A

K

G

N

K

G

G

V

I

V

D

V

I

D

Q

M

Y

R

V

L

I

P

P

K

K

E

E

G

G

A

A

P
x
N

I

L

W
|
W

F

F

D

D

M

L

L

M

A

A

I

I

P

P

K

A

G

D

A

A

K

K

N

A

P

A

E

D

D

N

A

A

Y

Y

T

A

F

F

I

I

N

D

Y

Y

L

L

Q

R

P

P

Q

E

V

V

I

I

A

A

P

K

V

V

S

S

D

D

F

Y

V

V

G

G

Y

Y

P

A

N

N

P

A

N

I

K

P

D

G

A

A

T

R

E

P

L

L

V

M

D

D

P

K

A

S

I

V

R

S

N

D

N

S

P

E

N

E

L

V

Y

Y

P

P

T

P

D

Q

A

A

A

V

M

L

G

D

T

K

L

L

Y

Y

T

V

L

S

Q

A

P

V

L

L

P

P

R

A

D

K

A

V

E

L

R

R

A

L

R

Q

T

T

R

R

A

T

W

W

T

T

K

R

I

I

K

K

S

T

G

G

T35 (≠ F34) binding spermidine
E181 (= E182) binding spermidine
D242 (≠ S243) binding spermidine
N269 (≠ P270) binding spermidine
W271 (= W272) mutation to F: Increases affinity for putrescine (to KD=1.12 uM).

8aszA Potf with mutations s87y and a182d in complex with agmatine
57% identity, 94% coverage: 23:365/366 of query aligns to 1:343/343 of 8aszA

query
sites
8aszA

A

A

E

E

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N
x
A

V
x
Y

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F
|
F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

D

A

A

D

A

V

M

R

G

A

T

K

L

L

Y

F

T

T

L

L

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding agmatine: W11 (= W33), S12 (≠ F34), Y14 (= Y36), W218 (≠ Y240), D221 (≠ S243), F250 (≠ W272), D252 (= D274), Y288 (= Y310)
binding calcium ion: A60 (≠ N83), Y61 (≠ V84), F250 (≠ W272), F251 (= F273)

6ye7A E.Coli's putrescine receptor potf complexed with cadaverine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/341 of 6ye7A

query
sites
6ye7A

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

D

A

A

D

A

V

M

R

G

A

T

K

L

L

Y

F

T

T

L

L

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding pentane-1,5-diamine: W9 (= W33), S10 (≠ F34), Y12 (= Y36), W216 (≠ Y240), D219 (≠ S243), F248 (≠ W272), D250 (= D274), Y286 (= Y310)

6ye6A E.Coli's putrescine receptor potf complexed with agmatine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/341 of 6ye6A

query
sites
6ye6A

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K
x
E

A

K

Q

E

N

-

T
|
T

Q

G

T

I

K
|
K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D
|
D

P

P

K

E

L

L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K
|
K

P
x
A

A
x
D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M
x
F

R
x
S

L
x
I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T
x
Q

F

F

I

L

N

N

Y

Y

L

L

Q
x
R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N
|
N

P

A

N

N

K
|
K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y
|
Y

P

P

T

P

D

A

A

D

A

V

M

R

G

A

T

K

L

L

Y

F

T

T

L

L

Q

K

P

V

L

Q

P

D

R

P

D
x
K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding agmatine: W9 (= W33), S10 (≠ F34), Y12 (= Y36), E157 (= E182), W216 (≠ Y240), D219 (≠ S243), F248 (≠ W272), D250 (= D274), Y286 (= Y310)
binding (2R)-1-methoxypropan-2-amine: T25 (= T50), D85 (= D110), W107 (≠ Y132), K174 (= K199), A175 (≠ P200), D176 (≠ A201), F238 (≠ M262), S239 (≠ R263), I240 (≠ L264), Q266 (≠ T290), R273 (≠ Q297), N288 (= N312), K291 (= K315), Y308 (= Y332), K326 (≠ D350)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: L91 (= L116), W107 (≠ Y132)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: E22 (≠ K46), K28 (= K53)

6ye0B E.Coli's putrescine receptor potf complexed with putrescine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/341 of 6ye0B

query
sites
6ye0B

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F
|
F

D
|
D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

D

A

A

D

A

V

M
x
R

G

A

T

K

L

L

Y

F

T

T

L

L

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding (2R)-1-methoxypropan-2-amine: F34 (= F59), D35 (= D60)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L91 (= L116), R314 (≠ M338)
binding 1,4-diaminobutane: W9 (= W33), S10 (≠ F34), Y12 (= Y36), W216 (≠ Y240), D219 (≠ S243), F248 (≠ W272), D250 (= D274), Y286 (= Y310)

1a99A Putrescine receptor (potf) from e. Coli (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/341 of 1a99A

query
sites
1a99A

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

D

A

A

D

A

V

M

R

G

A

T

K

L

L

Y

F

T

T

L

L

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding 1,4-diaminobutane: W9 (= W33), S10 (≠ F34), Y12 (= Y36), W216 (≠ Y240), D219 (≠ S243), F248 (≠ W272), D250 (= D274), Y286 (= Y310)

7oyxB E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/347 of 7oyxB

query
sites
7oyxB

E

Q

R
x
K

T
|
T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D
|
D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I
x
V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

S

F

Y

L

L

A

E

K

R

Q

Q

I

L

E

T

A
|
A

G
|
G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A
x
K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K
|
K

N
|
N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P
x
A

A
x
E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

D

A

A

D

A

V

M
x
R

G

A

T

K

L

L

Y

F

T

T

L

Q

Q

K

P
x
V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ R26), T3 (= T27), T10 (≠ F34), D11 (= D35), V33 (≠ I58), W107 (≠ Y132), K259 (= K283), N260 (= N284), R314 (≠ M338)
binding (2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine: K94 (≠ A119), V322 (≠ P346)
binding (2~{R})-1-(2-methoxyethoxy)propan-2-amine: A299 (≠ P323), E300 (≠ A324)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: A67 (= A92), G68 (= G93)
binding spermidine: W9 (= W33), T10 (≠ F34), Y12 (= Y36), S57 (= S82), E157 (= E182), W216 (≠ Y240), D219 (≠ S243), W248 (= W272), D250 (= D274), Y286 (= Y310)

7oywA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88l s247d in complex with spermidine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/348 of 7oywA

query
sites
7oywA

E

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K

E

A

K

Q

E

N

-

T

T

Q

G

T

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

Y

F

L

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N

N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T
x
P

D
x
A

A

D

A

V

M
x
R

G

A

T

K

L

L

Y

F

T

T

L

Q

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: P310 (≠ T334), A311 (≠ D335), R314 (≠ M338)
binding spermidine: W9 (= W33), T10 (≠ F34), Y12 (= Y36), S57 (= S82), E157 (= E182), W216 (≠ Y240), D219 (≠ S243), W248 (= W272), D250 (= D274), Y286 (= Y310)

7oyvA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88a s247d in complex with spermidine (see paper)
56% identity, 93% coverage: 25:365/366 of query aligns to 1:341/341 of 7oyvA

query
sites
7oyvA

E
x
Q

R
x
K

T
|
T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D

D

Y
|
Y

I

I

T

A

P

P

K

D

A

T

L

V

E

A

D

N

F

F

K
x
E

A
x
K

Q

E

N

-

T

T

Q

G

T

I

K
|
K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

Y

F

A

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

N

K

H

N

L

L

D

D

P

P

K

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

I

T

L

G

I

I

G

G

F

Y

N

N

P

V

D

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

A

E

D

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

S

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

H

Y

L

L

G

G

L

K

D

D

P

P

N

N

S

S

K

T

K

K

P

A

A

D

D

D

Y

Y

D

T

K

G

-

P

A

A

E

T

A

D

L

L

M

L

K

K

I

L

R

R

P

P

Y

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

K

K

E

E

A

A

K

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

K

K

N
|
N

P

K

E

D

D

E

A

A

Y

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

Q

R

P

P

Q

D

V

V

I

V

A

A

P

H

V

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

E

P

L

L

V

V

D

S

P

A

A

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T
x
P

D
x
A

A

D

A

V

M
x
R

G

A

T

K

L

L

Y

F

T

T

L

Q

Q

K

P

V

L

Q

P

D

R

P

D

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ R26), T3 (= T27), N260 (= N284)
binding (2~{R})-1-[(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-amine: Q1 (≠ E25), E22 (≠ K46), K23 (≠ A47), K28 (= K53)
binding (2R)-1-methoxypropan-2-amine: P310 (≠ T334), A311 (≠ D335), R314 (≠ M338)
binding spermidine: W9 (= W33), T10 (≠ F34), Y12 (= Y36), S57 (= S82), E157 (= E182), W216 (≠ Y240), D219 (≠ S243), W248 (= W272), D250 (= D274), Y286 (= Y310)

3ttnA Crystal structures of polyamine receptors spud and spue from pseudomonas aeruginosa (see paper)
55% identity, 92% coverage: 27:363/366 of query aligns to 1:335/335 of 3ttnA

query
sites
3ttnA

T

S

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D

D

Y
|
Y

I

I

T

A

P

P

K

T

A

T

L

L

E

K

D

D

F

F

K

-

A

T

Q

K

N

E

T

S

Q

G

T

I

K

D

L

V

V

S

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

T

L

L

E

E

A

G

K

K

L

L

L

V

T

S

G

G

N

H

S

S

G

G

Y

Y

D

D

V

I

V

V

P

P

S
|
S

N

N

V

N

F

F

L

L

A

G

K

K

Q

Q

I

I

E

Q

A

A

G

G

V

A

F

F

Q

Q

P

K

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

N

W

W

N

K

H

N

L

L

D

D

P

P

K

A

L

L

M

L

K

K

L

Q

I

L

E

E

A

V

N

S

D

D

P

P

G

G

N

N

K

Q

F

Y

A

A

V

V

P

P

Y

Y

M

L

Y

W

G

G

T

T

I

N

L

G

I

I

G

G

F

Y

N

N

P

V

D

A

K

K

V

V

K

K

A

E

A

V

L

L

G

G

A

-

D

D

A

Q

P

P

V

I

D

D

S

S

W

W

D

A

L

I

I

L

F

F

K

E

E

P

E

E

N

N

I

M

S

K

K

K

L

L

K

A

Q

K

C

C

G

G

V

V

A

A

L

F

L

M

D

D

S

S

P

G

S

D

E
|
E

I

M

L

L

P

P

L

A

A

A

L

L

Q

N

H

Y

L

L

G

G

L

L

D

D

P

P

N

N

S

T

K

H

K

D

P

P

A

K

D

D

Y

Y

D

K

K

K

A

A

E

E

A

E

L

V

L

L

M

T

K

K

I

V

R

R

P

P

Y

Y

I

V

T

S

Y

Y

F

F

H

H

S

S

K

K

Y

Y

M

I

A

S

D

D

I

L

A

A

N

N

G

G

D

N

I

I

C

C

V

V

A

A

V

F

G

G

Y
|
Y

S

S

G

G

S
x
D

F

V

S

F

Q

Q

A

A

N

A

R

R

A

A

K

E

E

E

A

A

K

G

N

K

G

G

V

I

V

D

V

I

D

Q

M

Y

R

V

L

I

P

P

K

K

E

E

G

G

A

A

P

N

I

L

W
|
W

F

F