SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PfGW456L13_928 FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_928 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

Q9I6J1 Putrescine-binding periplasmic protein SpuD from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
76% identity, 98% coverage: 7:370/370 of query aligns to 3:367/367 of Q9I6J1

query
sites
Q9I6J1

K

K

L

R

A

F

G

G

K

K

T

T

L

L

A

A

M

L

S

T

V

L

M

A

G

G

L

S

M

V

A

A

G

G

A

M

V

A

Q

Q

A

A

-

A

D

D

D

N

K

K

V

V

L

L

H

H

V

V

Y

Y

N

N

W

W

S
|
S

D
|
D

Y

Y

I

I

A

A

P

P

D

D

T

T

V

L

K

E

K

K

F

F

E

T

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

Y

D

D

S

S

N

N

E

E

T

V

L

L

E

E

A

A

K

K

L

L

A

A

G

G

K

K

S

S

G

G

Y

Y

D

D

I

V

V

V

P

P

S

S

N

N

N

S

F

F

L

L

A

A

K

K

Q

Q

I

I

K

K

A

A

G

G

V

V

Y

Y

Q

Q

K

K

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

N

W

W

K

K

N

N

L

L

N

N

T

K

D

D

L

L

L

M

K

H

A

T

V

L

S

E

V

V

S

S

D

D

P

P

G

G

N

N

E

E

H

H

A

A

F

I

P

P

Y

Y

M

M

W

W

G

G

S

T

I

I

G

G

I

I

G

G

F

Y

N

N

P

P

E

D

K

K

V

V

K

K

A

A

V

A

L

F

G

G

A

D

D

N

A

A

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P

T

V

N

D

S

S

W

W

D

D

L

L

L

V

F

F

K

K

P

P

E

E

N

N

A

I

A

Q

K

K

L

L

K

K

A

Q

C
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C

G

G

I

V

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S

F

F

L

L

D

D

S

S

P

P

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T

E

E

M

I

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L

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P

V

A

A

A

L

L

H

H

Y

Y

L

L

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G

Y

Y

P

K

T

P

D

D

S

T

Q

D

D

N

K

P

K

K

Q

E

L

L

A

K

E

A

A

A

E

E

A

E

L

L

F

F

L

L

K

K

I

I

R

R

P

P

S

Y

V

V

G

T

Y

Y

F

F

H

H

S

S

K

K

Y

Y

I

I

S

S

D

D

L

L

A

A

N

N

G

G

N

N

I

I

C
|
C

V

V

A

A

V

I

G

G

Y

Y

S

S

G

G

D
|
D

I

I

Y

Y

Q

Q

A

A

K

K

S

S

R

R

A

A

E

E

A

A

G

K

G

N

K

K

V

V

K

T

V

V

S

K

Y

Y

N

N

I

I

P

P

K

K

E

E

G

G

A

A

G

G

S

S

F
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F

Y

F

D
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D

M

M

V

V

A

A

I

I

P

P

K

K

D

D

A

A

E

E

N

N

V

T

E

E

G

G

A

A

Y

L

K

A

F

F

M

V

T

N

F

F

L

L

Q

M

K

K

P

P

E

E

I

I

M

M

A

A

E

E

I

I

T

T

N

D

A

V

V

V

R

Q

F

F

P

P

N

N

G

G

N

N

A

A

T

T

A

P

L

L

V

V

D

S

K

E

D

A

I

I

T

R

S

N

D

D

P

P

G

G

V

I

Y

Y

P

P

P

S

A

E

D

E

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V

L

M

A

K

K

K

L

L

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Y

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I

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A

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D

D

L

L

P

P

A

A

A

K

T

T

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Q

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R

I

A

M

M

T

T

R

R

S

S

W

W

T

T

K

K

I

I

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K

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S

G

G

K

K

SD 36:37 (= SD 39:40) binding
C173 (= C176) modified: Disulfide link with 236
C236 (= C239) modified: Disulfide link with 173
D244 (= D247) binding
F273 (= F276) mutation to W: Significant decrease in affinity for putrescine (to KD=0.75 uM).
D275 (= D278) binding

3ttmA Crystal structure of spud in complex with putrescine (see paper)
77% identity, 92% coverage: 29:369/370 of query aligns to 1:341/341 of 3ttmA

query
sites
3ttmA

D

D

D

N

K

K

V

V

L

L

H

H

V

V

Y

Y

N

N

W
|
W

S
|
S

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

L

K

E

K

K

F

F

E

T

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

Y

D

D

S

S

N

N

E

E

T

V

L

L

E

E

A

A

K

K

L

L

A

A

G

G

K

K

S

S

G

G

Y

Y

D

D

I

V

V

V

P

P

S

S

N

N

N

S

F

F

L

L

A

A

K

K

Q

Q

I

I

K

K

A

A

G

G

V

V

Y

Y

Q

Q

K

K

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

N

W

W

K

K

N

N

L

L

N

N

T

K

D

D

L

L

L

M

K

H

A

T

V

L

S

E

V

V

S

S

D

D

P

P

G

G

N

N

E

E

H

H

A

A

F

I

P

P

Y

Y

M

M

W

W

G

G

S

T

I

I

G

G

I

I

G

G

F

Y

N

N

P

P

E

D

K

K

V

V

K

K

A

A

V

A

L

F

G

G

A

D

D

N

A

A

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P

T

V

N

D

S

S

W

W

D

D

L

L

L

V

F

F

K

K

P

P

E

E

N

N

A

I

A

Q

K

K

L

L

K

K

A

Q

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

S

P

P

T

T

E

E

M

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I

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A

A

A

L

L

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H

Y

Y

L

L

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G

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Y

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K

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P

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D

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T

Q

D

D

N

K

P

K

K

Q

E

L

L

A

K

E

A

A

A

E

E

A

E

L

L

F

F

L

L

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K

I

I

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R

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P

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Y

V

V

G

T

Y

Y

F

F

H

H

S

S

K

K

Y

Y

I

I

S

S

D

D

L

L

A

A

N

N

G

G

N

N

I

I

C

C

V

V

A

A

V

I

G

G

Y
|
Y

S

S

G

G

D
|
D

I

I

Y

Y

Q

Q

A

A

K

K

S

S

R

R

A

A

E

E

A

A

G

K

G

N

K

K

V

V

K

T

V

V

S

K

Y

Y

N

N

I

I

P

P

K

K

E

E

G

G

A

A

G

G

S

S

F
|
F

Y

F

D
|
D

M

M

V

V

A

A

I

I

P

P

K

K

D

D

A

A

E

E

N

N

V

T

E

E

G

G

A

A

Y

L

K

A

F

F

M

V

T

N

F

F

L

L

Q

M

K

K

P

P

E

E

I

I

M

M

A

A

E

E

I

I

T

T

N

D

A

V

V

V

R

Q

F
|
F

P

P

N

N

G

G

N

N

A

A

T

T

A

P

L

L

V

V

D

S

K

E

D

A

I

I

T

R

S

N

D

D

P

P

G

G

V

I

Y

Y

P

P

P

S

A

E

D

E

V

V

L

M

A

K

K

K

L

L

Y

Y

A

T

I

F

A

P

D

D

L

L

P

P

A

A

A

K

T

T

Q

Q

R

R

I

A

M

M

T

T

R

R

S

S

W

W

T

T

K

K

I

I

K

K

S

S

G

G

binding 1,4-diaminobutane: W10 (= W38), S11 (= S39), D12 (= D40), Y13 (= Y41), Y216 (= Y244), D219 (= D247), F248 (= F276), D250 (= D278), F286 (= F314)

Q9I6J0 Spermidine-binding periplasmic protein SpuE from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
58% identity, 98% coverage: 10:370/370 of query aligns to 6:365/365 of Q9I6J0

query
sites
Q9I6J0

G

G

K

K

T

T

L

L

A

V

M

A

S

A

V

L

M

A

G

T

L

A

M

I

A

A

G

G

A

P

V

V

Q

Q

A

A

D

E

D

K

K

K

V

S

L

L

H

H

V

I

Y

Y

N

N

W

W

S
x
T

D

D

Y

Y

I

I

A

A

P

P

D

T

T

T

V

L

K

K

K

D

F

F

E

T

D

K

E

E

S

S

G

G

I

I

K

D

V

V

V

S

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

T

T

L

L

E

E

A

G

K

K

L

L

L

V

A

S

G

G

K

H

S

S

G

G

Y

Y

D

D

I

I

V

V

P

P

S

S

N

N

F

F

L

L

A

G

K

K

Q

Q

I

I

K

Q

A

A

G

G

V

A

Y

F

Q

Q

K

K

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

N

W

W

K

K

N

N

L

L

N

D

T

P

D

A

L

L

K

K

A

Q

V

L

S

E

V

V

S

S

D

D

P

P

G

G

N

N

E

Q

H

Y

A

A

F

V

P

P

Y

Y

M

L

W

W

G

G

S

T

I

N

G

G

I

I

G

G

F

Y

N

N

P

V

E

A

K

K

V

V

K

K

A

E

V

V

L

L

G

G

A

-

D

D

A

Q

P

P

T

I

N

D

S

S

W

W

D

A

L

I

L

L

F

F

K

E

P

P

E

E

N

N

A

M

A

K

K

K

L

L

K

A

A

K

C

C

G

G

I

V

S

A

F

F

L

M

D

D

S

S

P

G

T

D

E
|
E

M

M

I

L

P

P

V

A

A

A

L

L

H

N

Y

Y

L

L

G

G

Y

L

P

D

T

P

D

N

S

T

Q

H

D

D

K

P

K

K

Q

D

L

Y

A

K

E

K

A

A

E

E

A

E

L

V

F

L

L

T

K

K

I

V

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R

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P

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Y

V

V

G

S

Y

Y

F

F

H

H

S

S

K

K

Y

Y

I

I

S

S

D

D

L

L

A

A

N

N

G

G

N

N

I

I

C

C

V

V

A

A

V

F

G

G

Y

Y

S

S

G

G

D
|
D

I

V

Y

F

Q

Q

A

A

K

A

S

A

R

R

A

A

E

E

A

A

G

G

G

K

K

G

V

I

K

D

V

I

S

Q

Y

Y

N

V

I

I

P

P

K

K

E

E

G

G

A

A

G
x
N

S

L

F
x
W

Y

F

D

D

M

L

V

M

A

A

I

I

P

P

K

A

D

D

A

A

E

K

N

A

V

A

E

D

G

N

A

A

Y

Y

K

A

F

F

M

I

T

D

F

Y

L

L

Q

L

K

R

P

P

E

E

I

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M

I

A

A

E

K

I

V

T

S

N

D

A

Y

V

V

R

G

F

Y

P

A

N

N

G

A

N

I

A

P

A

G

A

A

T

R

A

P

L

L

V

M

D

D

K

K

D

S

I

V

T

S

S

D

D

S

P

E

G

E

V

V

Y

Y

P

P

A

Q

D

A

V

V

L

L

A

D

K

K

L

L

Y

Y

A

V

I

S

A

A

D

V

L

L

P

P

A

A

A

K

T

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Q

L

R

R

I

L

M

Q

T

T

R

R

S

T

W

W

T

T

K

R

I

I

K

K

S

T

G

G

K

K

T35 (≠ S39) binding
E181 (= E186) binding
D242 (= D247) binding
N269 (≠ G274) binding
W271 (≠ F276) mutation to F: Increases affinity for putrescine (to KD=1.12 uM).

P31133 Putrescine-binding periplasmic protein PotF from Escherichia coli (strain K12) (see 3 papers)
55% identity, 100% coverage: 1:370/370 of query aligns to 1:370/370 of P31133

query
sites
P31133

M
|
M

K
x
T

A
|
A

L
|
L

G
x
N

K
|
K

-
x
W

L
|
L

A
x
S

G
|
G

K

-

T

-

L
|
L

L
x
V

A
|
A

M
x
G

S
x
A

V
x
L

M
|
M

G
x
A

L
x
V

M
x
S

A
x
V

G
|
G

A
x
T

V
x
L

Q
x
A

A

A

D

E

D

Q

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W

W

S
|
S

D

D

Y

Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L

M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

N

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L

L

K

K

A

L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W

W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C
|
C

G

G

I

V

S

S

F

F

L

L

D

D

S

A

P

P

T

E

E

E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C
|
C

V

V

A

A

V

I

G

G

Y

W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F

F

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F

Y

P

A

N

N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y

Y

P

P

A

A

D

D

V

V

L

R

A

A

K

K

L

L

Y

F

A

T

I

L

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

K

K

1:26 (vs. 1:27, 39% identical) signal peptide
S38 (= S39) binding
C175 (= C176) modified: Disulfide link with 239
C239 (= C239) modified: Disulfide link with 175
D247 (= D247) binding
D278 (= D278) binding

3ttnA Crystal structures of polyamine receptors spud and spue from pseudomonas aeruginosa (see paper)
59% identity, 91% coverage: 33:367/370 of query aligns to 2:335/335 of 3ttnA

query
sites
3ttnA

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
x
T

D

D

Y
|
Y

I

I

A

A

P

P

D

T

T

T

V

L

K

K

K

D

F

F

E

T

D

K

E

E

S

S

G

G

I

I

K

D

V

V

V

S

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

T

T

L

L

E

E

A

G

K

K

L

L

L

V

A

S

G

G

K

H

S

S

G

G

Y

Y

D

D

I

I

V

V

P

P

S
|
S

N

N

F

F

L

L

A

G

K

K

Q

Q

I

I

K

Q

A

A

G

G

V

A

Y

F

Q

Q

K

K

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

N

W

W

K

K

N

N

L

L

N

D

T

P

D

A

L

L

K

K

A

Q

V

L

S

E

V

V

S

S

D

D

P

P

G

G

N

N

E

Q

H

Y

A

A

F

V

P

P

Y

Y

M

L

W

W

G

G

S

T

I

N

G

G

I

I

G

G

F

Y

N

N

P

V

E

A

K

K

V

V

K

K

A

E

V

V

L

L

G

G

A

-

D

D

A

Q

P

P

T

I

N

D

S

S

W

W

D

A

L

I

L

L

F

F

K

E

P

P

E

E

N

N

A

M

A

K

K

K

L

L

K

A

A

K

C

C

G

G

I

V

S

A

F

F

L

M

D

D

S

S

P

G

T

D

E
|
E

M

M

I

L

P

P

V

A

A

A

L

L

H

N

Y

Y

L

L

G

G

Y

L

P

D

T

P

D

N

S

T

Q

H

D

D

K

P

K

K

Q

D

L

Y

A

K

E

K

A

A

E

E

A

E

L

V

F

L

L

T

K

K

I

V

R

R

P

P

S

Y

V

V

G

S

Y

Y

F

F

H

H

S

S

K

K

Y

Y

I

I

S

S

D

D

L

L

A

A

N

N

G

G

N

N

I

I

C

C

V

V

A

A

V

F

G

G

Y
|
Y

S

S

G

G

D
|
D

I

V

Y

F

Q

Q

A

A

K

A

S

A

R

R

A

A

E

E

A

A

G

G

G

K

K

G

V

I

K

D

V

I

S

Q

Y

Y

N

V

I

I

P

P

K

K

E

E

G

G

A

A

G
x
N

S

L

F
x
W

Y

F

D
|
D

M

L

V

M

A

A

I

I

P

P

K

A

D

D

A

A

E

K

N

A

V

A

E

D

G

N

A

A

Y

Y

K

A

F

F

M

I

T

D

F

Y

L

L

Q

L

K

R

P

P

E

E

I

V

M

I

A

A

E

K

I

V

T

S

N

D

A

Y

V

V

R

G

F
x
Y

P

A

N

N

G

A

N

I

A

P

A

G

A

A

T

R

A

P

L

L

V

M

D

D

K

K

D

S

I

V

T

S

S

D

D

S

P

E

G

E

V

V

Y

Y

P

P

A

Q

D

A

V

V

L

L

A

D

K

K

L

L

Y

Y

A

V

I

S

A

A

D

V

L

L

P

P

A

A

A

K

T

V

Q

L

R

R

I

L

M

Q

T

T

R

R

S

T

W

W

T

T

K

R

I

I

K

K

binding spermidine: W7 (= W38), T8 (≠ S39), Y10 (= Y41), S55 (= S86), E154 (= E186), Y212 (= Y244), D215 (= D247), N242 (≠ G274), W244 (≠ F276), D246 (= D278), Y282 (≠ F314)

6ye7A E.Coli's putrescine receptor potf complexed with cadaverine (see paper)
56% identity, 92% coverage: 31:369/370 of query aligns to 2:341/341 of 6ye7A

query
sites
6ye7A

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
|
S

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L

M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

N

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L

L

K

K

A

L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W

W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

A

P

P

T

E

E

E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
|
F

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N

N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y

Y

P

P

A

A

D

D

V

V

L

R

A

A

K

K

L

L

Y

F

A

T

I

L

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding pentane-1,5-diamine: W9 (= W38), S10 (= S39), D11 (= D40), Y12 (= Y41), W216 (≠ Y244), D219 (= D247), F248 (= F276), D250 (= D278), Y286 (≠ F314)

6ye6A E.Coli's putrescine receptor potf complexed with agmatine (see paper)
56% identity, 92% coverage: 31:369/370 of query aligns to 2:341/341 of 6ye6A

query
sites
6ye6A

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
|
S

D
|
D

Y
|
Y

I
|
I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E
|
E

D
x
K

E
|
E

S
x
T

G
|
G

I

I

K
|
K

V

V

Y
|
Y

D

D

V
|
V

F
|
F

D
|
D

S

S

N
|
N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L

M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

N

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N
x
D

T

P

D

E

L

L

K

K

A
x
L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W
|
W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

A

P

P

T

E

E
|
E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S
x
T

Q
x
K

D
x
A

K
x
D

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

S
|
S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y
x
W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y
x
F

N
x
S

I
|
I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
|
F

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K
x
Q

F

F

M

L

T
x
N

F
x
Y

L

L

Q

L

K
x
R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N
|
N

G
x
A

N

N

A
x
K

A

A

T
|
T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G
|
G

V

I

Y
|
Y

P

P

P
|
P

A
|
A

D

D

V

V

L
x
R

A

A

K

K

L
|
L

Y

F

A

T

I

L

A

K

D

V

L

Q

P

D

A

P

A
x
K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding agmatine: W9 (= W38), S10 (= S39), D11 (= D40), Y12 (= Y41), N37 (= N66), S57 (= S86), E157 (= E186), W216 (≠ Y244), D219 (= D247), F248 (= F276), D250 (= D278), Y286 (≠ F314)
binding (2R)-1-methoxypropan-2-amine: S10 (= S39), S10 (= S39), D11 (= D40), I13 (= I42), E24 (= E53), T25 (≠ S54), Y31 (= Y60), V33 (= V62), V33 (= V62), F34 (= F63), D35 (= D64), D85 (≠ N114), W107 (= W136), T173 (≠ S201), K174 (≠ Q202), A175 (≠ D203), D176 (≠ K204), S199 (= S227), W221 (≠ Y249), F238 (≠ Y266), S239 (≠ N267), I240 (= I268), Q266 (≠ K294), N269 (≠ T297), Y270 (≠ F298), R273 (≠ K301), N288 (= N316), A289 (≠ G317), K291 (≠ A319), T294 (= T322), G306 (= G334), Y308 (= Y336), P310 (= P338), A311 (= A339), R314 (≠ L342), K326 (≠ A354)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: E22 (= E51), K23 (≠ D52), G26 (= G55), L91 (≠ A120), W107 (= W136), R314 (≠ L342), L317 (= L345)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: E22 (= E51), K28 (= K57)

6ye0A E.Coli's putrescine receptor potf complexed with putrescine (see paper)
56% identity, 92% coverage: 31:369/370 of query aligns to 2:341/341 of 6ye0A

query
sites
6ye0A

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
|
S

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V
|
V

F
|
F

D
|
D

S

S

N

N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L
x
M

A

A

G
|
G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

N

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A
|
A

G
|
G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L
|
L

L

L

K

K

A
x
L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W
|
W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

A

P

P

T

E

E

E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

S
|
S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
|
F

Y
x
F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N

N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y

Y

P

P

A

A

D

D

V

V

L
x
R

A

A

K

K

L

L

Y

F

A

T

I

L

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding (2R)-1-methoxypropan-2-amine: S10 (= S39), V33 (= V62), F34 (= F63), D35 (= D64), S199 (= S227)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: M45 (≠ L74), G47 (= G76), A67 (= A96), G68 (= G97), L88 (= L117), L91 (≠ A120), W107 (= W136), F249 (≠ Y277), R314 (≠ L342)
binding 1,4-diaminobutane: W9 (= W38), S10 (= S39), D11 (= D40), Y12 (= Y41), W216 (≠ Y244), D219 (= D247), F248 (= F276), D250 (= D278), Y286 (≠ F314)

1a99A Putrescine receptor (potf) from e. Coli (see paper)
56% identity, 92% coverage: 31:369/370 of query aligns to 2:341/341 of 1a99A

query
sites
1a99A

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
|
S

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N

N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L

M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S

S

N

A

N

S

F

F

L

L

A

E

K

R

Q

Q

I

L

K

T

A

A

G

G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L

L

K

K

A

L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W

W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

A

P

P

T

E

E

E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
|
F

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N

N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y

Y

P

P

A

A

D

D

V

V

L

R

A

A

K

K

L

L

Y

F

A

T

I

L

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

binding 1,4-diaminobutane: W9 (= W38), S10 (= S39), D11 (= D40), Y12 (= Y41), W216 (≠ Y244), D219 (= D247), F248 (= F276), D250 (= D278), Y286 (≠ F314)

7oyxB E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
56% identity, 92% coverage: 31:370/370 of query aligns to 2:342/347 of 7oyxB

query
sites
7oyxB

K
|
K

V
x
T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
x
T

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V
|
V

F

F

D

D

S

S

N
|
N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L
x
M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

N

S

F

Y

L

L

A
x
E

K
x
R

Q

Q

I

L

K
x
T

A
|
A

G
|
G

V

V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L

L

K

K

A
x
L

V

V

S

A

V
x
K

S
x
H

D

D

P
|
P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W
|
W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

D

P

P

T

E

E
|
E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
x
W

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E
x
K

N
|
N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N

N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D
x
S

K
x
A

D
x
E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y

Y

P

P

A

A

D

D

V

V

L
x
R

A

A

K

K

L

L

Y

F

A

T

I

Q

A

K

D
x
V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

K

K

binding (2~{S})-1-methoxypropan-2-amine: K2 (= K31), T3 (≠ V32), T10 (≠ S39), D11 (= D40), V33 (= V62), L91 (≠ A120), W107 (= W136), K259 (≠ E287), N260 (= N288), R314 (≠ L342)
binding (2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine: E62 (≠ A91), R63 (≠ K92), T66 (≠ K95), K94 (≠ V123), H95 (≠ S124), P97 (= P126), V322 (≠ D350)
binding (2~{R})-1-(2-methoxyethoxy)propan-2-amine: S298 (≠ D326), A299 (≠ K327), E300 (≠ D328)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: M45 (≠ L74), A67 (= A96), G68 (= G97)
binding spermidine: W9 (= W38), T10 (≠ S39), D11 (= D40), Y12 (= Y41), N37 (= N66), S57 (= S86), E157 (= E186), W216 (≠ Y244), D219 (= D247), W248 (≠ F276), D250 (= D278), Y286 (≠ F314)

7oyxA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
56% identity, 92% coverage: 31:370/370 of query aligns to 2:342/347 of 7oyxA

query
sites
7oyxA

K
|
K

V
x
T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
x
T

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N
|
N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L
x
M

A

A

G
|
G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

N

S

F

Y

L

L

A

E

K

R

Q

Q

I

L

K

T

A
|
A

G
|
G

V
|
V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L
|
L

L

L

K

K

A
x
L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W
|
W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

D

P

P

T

E

E
|
E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
x
W

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E
x
K

N
|
N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P

A

N
|
N

G

A

N

N

A

K

A

A

T

T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y
|
Y

P

P

A

A

D

D

V

V

L
x
R

A

A

K

K

L

L

Y

F

A

T

I

Q

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

K

K

binding (2~{S})-1-methoxypropan-2-amine: K2 (= K31), T3 (≠ V32), M45 (≠ L74), G47 (= G76), V69 (= V98), K259 (≠ E287), N260 (= N288)
binding (2~{S})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L88 (= L117), L91 (≠ A120), W107 (= W136), N288 (= N316), Y308 (= Y336), R314 (≠ L342)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: A67 (= A96), G68 (= G97)
binding spermidine: W9 (= W38), T10 (≠ S39), D11 (= D40), Y12 (= Y41), N37 (= N66), S57 (= S86), E157 (= E186), W216 (≠ Y244), D219 (= D247), W248 (≠ F276), D250 (= D278), Y286 (≠ F314)

7oywA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88l s247d in complex with spermidine (see paper)
56% identity, 92% coverage: 31:370/370 of query aligns to 2:342/348 of 7oywA

query
sites
7oywA

K

K

V

T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
x
T

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E

E

D

K

E

E

S

T

G

G

I

I

K

K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N
|
N

E

E

T

V

L

L

E

E

A

G

K

K

L
|
L

L
x
M

A

A

G
|
G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

N

Y

F

L

L

L

A

E

K

R

Q

Q

I

L

K

T

A
|
A

G
|
G

V
|
V

Y

F

Q

Q

K

P

L

L

D

D

K

K

S

S

K

K

L

L

P

P

N

E

W

W

K

K

N

N

L

L

N

D

T

P

D

E

L
|
L

L

L

K

K

A

L

V

V

S

A

V

K

S

H

D

D

P

P

G

D

N

N

E

K

H

F

A

A

F

M

P

P

Y

Y

M

M

W
|
W

G

A

S

T

I

T

G

G

I

I

G

G

F

Y

N

N

P

V

E

D

K

K

V

V

K

K

A

A

V

V

L

L

G

G

A

E

D

N

A

A

P

P

T

V

N

D

S

S

W

W

D

D

L

L

L

I

F

L

K

K

P

P

E

E

N

N

A

L

A

E

K

K

L

L

K

K

A

S

C

C

G

G

I

V

S

S

F

F

L

L

D

D

S

D

P

P

T

E

E
|
E

M

V

I

F

P

A

V

T

A

V

L

L

H

N

Y

Y

L

L

G

G

Y

K

-

D

P

P

T

N

D

S

S

T

Q

K

D

A

K

D

Q

Y

L

T

A

G

E

P

A

A

E

T

A

D

L

L

F

L

L

L

K

K

I

L

R

R

P

P

S

N

V

I

G

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

I

I

S

N

D

D

L

L

A

A

N

N

G

G

N

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

D
|
D

I

V

Y

W

Q

Q

A

A

K

S

S

N

R

R

A

A

E

K

E

E

A

A

G

K

G

N

K

G

V

V

K

N

V

V

S

S

Y

F

N

S

I

I

P

P

K

K

E

E

G

G

A

A

G

M

S

A

F
x
W

Y

F

D
|
D

M

V

V

F

A

A

I

M

P

P

K

A

D

D

A

A

E

K

N

N

V

K

E

D

G

E

A

A

Y

Y

K

Q

F

F

M

L

T

N

F

Y

L

L

Q

L

K

R

P

P

E

D

I

V

M

V

A

A

E

H

I

I

T

S

N

D

A

H

V

V

R

F

F
x
Y

P
x
A

N
|
N

G

A

N
|
N

A
x
K

A

A

T
|
T

A

P

L

L

V

V

D

S

K

A

D

E

I

V

T

R

S

E

D

N

P

P

G

G

V

I

Y
|
Y

P

P

P
|
P

A
|
A

D
|
D

V

V

L
x
R

A

A

K

K

L

L

Y

F

A

T

I

Q

A

K

D

V

L

Q

P

D

A

P

A

K

T

I

Q

D

R

R

I

V

M

R

T

T

R

R

S

A

W

W

T

T

K

K

I

V

K

K

S

S

G

G

K

K

binding (2~{S})-1-methoxypropan-2-amine: L44 (= L73), M45 (≠ L74), G47 (= G76), A67 (= A96), G68 (= G97), V69 (= V98)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: P310 (= P338), A311 (= A339), D312 (= D340), R314 (≠ L342)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L88 (= L117), W107 (= W136), A287 (≠ P315), N288 (= N316), N290 (= N318), K291 (≠ A319), T294 (= T322), Y308 (= Y336)
binding spermidine: W9 (= W38), T10 (≠ S39), D11 (= D40), Y12 (= Y41), N37 (= N66), S57 (= S86), E157 (= E186), W216 (≠ Y244), D219 (= D247), W248 (≠ F276), D250 (= D278), Y286 (≠ F314)

7oyvA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88a s247d in complex with spermidine (see paper)
56% identity, 92% coverage: 31:369/370 of query aligns to 2:341/341 of 7oyvA

query
sites
7oyvA

K
|
K

V
x
T

L

L

H

H

V

I

Y

Y

N

N

W
|
W

S
x
T

D
|
D

Y
|
Y

I

I

A

A

P

P

D

D

T

T

V

V

K

A

K

N

F

F

E
|
E

D
x
K

E

E

S

T

G
|
G

I
|
I

K
|
K

V

V

Y

Y

D

D

V

V

F

F

D

D

S

S

N
|
N

E

E

T

V

L

L

E

E

A

G

K

K

L

L

L

M

A

A

G

G

K

S

S

T

G

G

Y

F

D

D

I

L

V

V

P

P

S
|
S

N

A

N
x
Y

F

A

L

L

A

E

K

R

Q

Q

I

L