SitesBLAST
Comparing RR42_RS01280 FitnessBrowser__Cup4G11:RR42_RS01280 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
37% identity, 97% coverage: 5:545/559 of query aligns to 5:452/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G11), G13 (= G13), S14 (= S14), A15 (= A15), E35 (= E35), A36 (≠ T36), W47 (≠ Y63), P65 (= P81), G67 (= G83), V180 (= V232), A214 (= A266), G215 (= G267), A218 (≠ G270), T270 (≠ V324), Y391 (= Y484), A424 (= A517), I435 (≠ T528), N436 (= N529)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
37% identity, 96% coverage: 5:543/559 of query aligns to 24:569/578 of 5nccA
- active site: R347 (≠ L333), L420 (≠ V395), I421 (≠ C396), S507 (≠ I483), A509 (≠ H485), G552 (= G526), Q553 (≠ N527)
- binding flavin-adenine dinucleotide: G30 (= G11), G32 (= G13), T33 (≠ S14), A34 (= A15), L53 (= L34), E54 (= E35), A55 (≠ T36), F74 (≠ L55), W80 (≠ Y63), A98 (≠ P81), G100 (= G83), G105 (= G88), S106 (= S89), N110 (= N93), A111 (= A94), T112 (≠ M95), L113 (≠ I96), V238 (= V232), A278 (= A266), H282 (≠ G270), L286 (= L274), N508 (≠ Y484), Q553 (≠ N527), T554 (= T528), G555 (≠ N529), V558 (≠ A532)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
37% identity, 96% coverage: 5:543/559 of query aligns to 84:636/654 of A0A248QE08
- TA 93:94 (≠ SA 14:15) binding
- E114 (= E35) binding
- L162 (≠ G85) binding
- S166 (= S89) binding
- NATL 170:173 (≠ NAMI 93:96) binding
- V298 (= V232) binding
- C432 (≠ A352) binding
- R451 (≠ H372) binding
- Y466 (vs. gap) binding
- Q486 (≠ H394) binding
- G622 (≠ N529) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
37% identity, 96% coverage: 5:543/559 of query aligns to 8:560/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H372), N499 (≠ Y484)
- binding flavin-adenine dinucleotide: G14 (= G11), G16 (= G13), T17 (≠ S14), A18 (= A15), L37 (= L34), E38 (= E35), A39 (≠ T36), F58 (≠ L55), W64 (≠ Y63), A82 (≠ P81), G89 (= G88), S90 (= S89), N94 (= N93), A95 (= A94), T96 (≠ M95), L97 (≠ I96), M191 (≠ T193), V222 (= V232), C264 (≠ S265), A265 (= A266), G266 (= G267), H269 (≠ G270), N499 (≠ Y484), A534 (= A517), Q544 (≠ N527), T545 (= T528), G546 (≠ N529)
- binding heptadecane: V377 (= V374), G379 (= G376), M380 (= M377), G386 (vs. gap), T389 (vs. gap), Y390 (vs. gap), F393 (vs. gap), T408 (≠ S392), Q410 (≠ H394)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
36% identity, 97% coverage: 5:544/559 of query aligns to 14:527/527 of 2jbvA
- active site: I333 (≠ A354), P377 (≠ V395), N378 (≠ C396), V464 (≠ I483), H466 (= H485), V509 (≠ G526), N510 (= N527)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G13), S23 (= S14), E44 (= E35), A45 (≠ T36), W71 (≠ Y63), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ L92), N100 (= N93), S101 (≠ A94), I103 (= I96), R231 (≠ Q231), A232 (≠ V232), T269 (≠ A266), G270 (= G267), D273 (≠ G270), V464 (≠ I483), Y465 (= Y484), H466 (= H485), D499 (= D516), A500 (= A517), N510 (= N527), P511 (≠ T528), N512 (= N529), V515 (≠ A532)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
37% identity, 97% coverage: 5:544/559 of query aligns to 14:527/532 of 4mjwA
- active site: I333 (≠ A354), P377 (≠ V395), N378 (≠ C396), V464 (≠ I483), H466 (= H485), V509 (≠ G526), N510 (= N527)
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), S23 (= S14), E44 (= E35), A45 (≠ T36), W71 (≠ Y63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ L92), N100 (= N93), S101 (≠ A94), I103 (= I96), R231 (≠ Q231), A232 (≠ V232), T269 (≠ A266), G270 (= G267), D273 (≠ G270), Y465 (= Y484), H466 (= H485), A500 (= A517), N510 (= N527), P511 (≠ T528), N512 (= N529), V515 (≠ A532)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
37% identity, 97% coverage: 5:544/559 of query aligns to 14:527/530 of 3ljpA
- active site: I333 (≠ A354), P377 (≠ V395), N378 (≠ C396), A464 (≠ I483), H466 (= H485), V509 (≠ G526), N510 (= N527)
- binding dihydroflavine-adenine dinucleotide: G22 (= G13), S23 (= S14), E44 (= E35), A45 (≠ T36), W71 (≠ Y63), R89 (= R82), A90 (≠ G83), G95 (= G88), C96 (≠ S89), H99 (≠ L92), N100 (= N93), S101 (≠ A94), I103 (= I96), A232 (≠ V232), T269 (≠ A266), D273 (≠ G270), Y465 (= Y484), H466 (= H485), D499 (= D516), A500 (= A517), N510 (= N527), P511 (≠ T528), N512 (= N529), V515 (≠ A532)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
36% identity, 97% coverage: 2:543/559 of query aligns to 3:527/531 of E4QP00
- V101 (≠ L92) mutation to H: Abolishes activity.
- M103 (≠ A94) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ H394) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ C396) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ I483) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y484) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H485) mutation to A: Abolishes activity.
- N511 (= N527) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
36% identity, 96% coverage: 5:543/559 of query aligns to 2:523/525 of 4udqA
- active site: L331 (≠ T344), F364 (≠ V395), W365 (≠ C396), V461 (≠ I483), H463 (= H485), A506 (≠ G526), N507 (= N527)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), T11 (≠ S14), A12 (= A15), E32 (= E35), A33 (≠ T36), W64 (≠ Y65), G88 (= G83), G93 (= G88), G94 (≠ S89), N98 (= N93), M99 (≠ A94), V101 (≠ I96), V229 (= V232), T261 (≠ S265), A262 (= A266), W462 (≠ Y484), H463 (= H485), A497 (= A517), N507 (= N527), T508 (= T528), N509 (= N529), T512 (≠ A532)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
31% identity, 97% coverage: 5:544/559 of query aligns to 2:562/565 of 5oc1A
- active site: V339 (≠ A334), N413 (≠ V395), A414 (≠ C396), I499 (= I483), H501 (= H485), A544 (≠ G526), H545 (≠ N527)
- binding 4-methoxybenzoic acid: Y91 (≠ A94), I356 (≠ F351), I390 (≠ H372), F396 (≠ A378), T412 (≠ H394), I499 (= I483), H501 (= H485), H545 (≠ N527)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), N11 (≠ S14), A12 (= A15), E32 (= E35), A33 (≠ T36), W60 (≠ Y63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ A94), V93 (≠ I96), V230 (= V232), S270 (= S265), A271 (= A266), G272 (= G267), F500 (≠ Y484), H545 (≠ N527), T546 (= T528), Q547 (≠ N529), I550 (≠ A532)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
31% identity, 97% coverage: 5:544/559 of query aligns to 2:562/565 of 3fimB
- active site: V339 (≠ A334), N413 (≠ V395), A414 (≠ C396), I499 (= I483), H501 (= H485), A544 (≠ G526), H545 (≠ N527)
- binding flavin-adenine dinucleotide: G8 (= G11), N11 (≠ S14), A12 (= A15), E32 (= E35), A33 (≠ T36), W60 (≠ Y63), P78 (= P81), G80 (= G83), G85 (= G88), S86 (= S89), H90 (≠ N93), Y91 (≠ A94), V93 (≠ I96), V230 (= V232), S270 (= S265), A271 (= A266), F500 (≠ Y484), H501 (= H485), H545 (≠ N527), T546 (= T528), Q547 (≠ N529), I550 (≠ A532)
8bxlB Patulin synthase from penicillium expansum
33% identity, 96% coverage: 4:538/559 of query aligns to 13:581/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G11), G22 (= G13), T23 (≠ S14), A24 (= A15), E44 (= E35), A45 (≠ T36), W80 (≠ Y63), G100 (= G83), G105 (= G88), S106 (= S89), R109 (≠ L92), N110 (= N93), Y111 (≠ A94), A113 (≠ I96), L253 (≠ Q231), A254 (≠ V232), A288 (= A266), Q292 (≠ G270), F525 (≠ Y484), D559 (= D516), A560 (= A517), H570 (≠ N527), P571 (≠ T528), Q572 (≠ N529), L575 (≠ A532)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 95% coverage: 6:537/559 of query aligns to 3:498/509 of 3t37A
- active site: F360 (≠ V395), G361 (≠ C396), H444 (≠ I483), H446 (= H485), G487 (= G526), P488 (≠ N527)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), S11 (= S14), A12 (= A15), E32 (= E35), A33 (≠ T36), W58 (≠ Y63), R77 (= R82), G78 (= G83), R79 (= R84), G83 (= G88), S84 (= S89), H88 (≠ N93), A89 (= A94), G91 (≠ I96), R217 (≠ Q231), V218 (= V232), A251 (= A266), E255 (≠ G270), H445 (≠ Y484), A478 (= A517), P488 (≠ N527), I489 (≠ T528), H490 (≠ N529)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 95% coverage: 6:537/559 of query aligns to 3:498/508 of 4ha6A
- active site: F360 (≠ V395), G361 (≠ C396), H444 (≠ I483), H446 (= H485), G487 (= G526), P488 (≠ N527)
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), S11 (= S14), A12 (= A15), E32 (= E35), A33 (≠ T36), W58 (≠ Y63), R77 (= R82), G78 (= G83), G83 (= G88), S84 (= S89), L87 (= L92), H88 (≠ N93), A89 (= A94), M90 (= M95), G91 (≠ I96), V218 (= V232), A251 (= A266), G252 (= G267), E255 (≠ G270), H445 (≠ Y484), A478 (= A517), P488 (≠ N527), I489 (≠ T528), H490 (≠ N529)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A94), S314 (≠ R340), H444 (≠ I483), H446 (= H485)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
29% identity, 96% coverage: 5:543/559 of query aligns to 41:597/602 of Q3L245
- N100 (≠ G64) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ L92) modified: Tele-8alpha-FAD histidine
- N344 (= N303) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H485) active site, Proton acceptor
- H581 (≠ N527) active site
Sites not aligning to the query:
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
29% identity, 96% coverage: 5:543/559 of query aligns to 16:572/577 of 4h7uA
- active site: A343 (= A334), V426 (≠ C396), Y510 (≠ I483), H512 (= H485), A555 (≠ G526), H556 (≠ N527)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G11), G24 (= G13), T25 (≠ S14), A26 (= A15), E46 (= E35), A47 (≠ T36), W74 (≠ Y63), G99 (= G88), C100 (≠ S89), H103 (≠ L92), N104 (= N93), G105 (≠ A94), V107 (≠ I96), L242 (≠ Q231), V243 (= V232), G282 (≠ A266), G283 (= G267), A286 (≠ G270), H512 (= H485), A546 (= A517), H556 (≠ N527), T557 (= T528), Q558 (≠ N529), V561 (≠ A532)
6h3oF Alcohol oxidase from phanerochaete chrysosporium mutant f101s (see paper)
29% identity, 96% coverage: 6:543/559 of query aligns to 7:619/650 of 6h3oF
- binding flavin-adenine dinucleotide: P15 (≠ S14), E37 (≠ T36), G38 (= G37), N90 (≠ R84), I91 (≠ G85), G94 (= G88), G95 (≠ S89), N99 (= N93), S100 (≠ A94), Q101 (≠ M95), M102 (≠ I96), R227 (≠ Q231), V228 (= V232), S271 (≠ A266), I279 (≠ L274), W559 (≠ Y484), L593 (≠ A517), N603 (= N527), T604 (= T528), Y605 (≠ N529), A608 (= A532)
6h3gB Alcohol oxidase from phanerochaete chrysosporium (see paper)
29% identity, 96% coverage: 6:543/559 of query aligns to 5:617/642 of 6h3gB
- binding flavin-adenine dinucleotide: P13 (≠ S14), E35 (≠ T36), G36 (= G37), A87 (≠ G83), G92 (= G88), G93 (≠ S89), N97 (= N93), F98 (≠ A94), M100 (≠ I96), R225 (≠ Q231), V226 (= V232), S269 (≠ A266), I277 (≠ L274), W557 (≠ Y484), L591 (≠ A517), N601 (= N527), T602 (= T528), Y603 (≠ N529), A606 (= A532)
- binding glycerol: R54 (≠ A53), Q57 (≠ P56), N59 (≠ A58)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
30% identity, 98% coverage: 3:548/559 of query aligns to 3:568/570 of 4yntA
- active site: V342 (≠ Y339), F413 (≠ V395), W414 (≠ C396), N502 (≠ I483), H504 (= H485), G546 (= G526), H547 (≠ N527)
- binding dihydroflavine-adenine dinucleotide: G13 (= G13), T14 (≠ S14), S15 (≠ A15), E35 (= E35), A36 (≠ T36), F56 (≠ R57), W62 (≠ Y63), R80 (≠ P81), G82 (= G83), G87 (= G88), T88 (≠ S89), N92 (= N93), G93 (≠ A94), M94 (= M95), A95 (≠ I96), A234 (≠ V232), A274 (= A266), R278 (≠ G270), F503 (≠ Y484), A537 (= A517), H547 (≠ N527), L548 (≠ T528), V549 (≠ N529), L552 (≠ A532)
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
30% identity, 98% coverage: 3:548/559 of query aligns to 2:567/569 of 4ynuA
- active site: V341 (≠ Y339), F412 (≠ V395), W413 (≠ C396), N501 (≠ I483), H503 (= H485), G545 (= G526), H546 (≠ N527)
- binding flavin-adenine dinucleotide: G12 (= G13), T13 (≠ S14), S14 (≠ A15), E34 (= E35), A35 (≠ T36), Y51 (≠ A52), F55 (≠ R57), W61 (≠ Y63), R79 (≠ P81), G81 (= G83), G86 (= G88), T87 (≠ S89), N91 (= N93), G92 (≠ A94), T232 (≠ Q231), A233 (≠ V232), A273 (= A266), G274 (= G267), R277 (≠ G270), F502 (≠ Y484), A536 (= A517), H546 (≠ N527), L547 (≠ T528), V548 (≠ N529), L551 (≠ A532)
- binding D-glucono-1,5-lactone: Y51 (≠ A52), E411 (≠ H394), A496 (= A478), N497 (≠ H479), R499 (≠ D481), R499 (≠ D481), N501 (≠ I483), H503 (= H485), H546 (≠ N527)
Query Sequence
>RR42_RS01280 FitnessBrowser__Cup4G11:RR42_RS01280
METTFDYLVVGGGSAGCALAGRLADSGSDSVAVLETGGHDHHVLVSTPAGCAAMLPRAGS
HNYGYRTVPQPGLNGRRGYQPRGRGLGGSSSLNAMIYTRGTPADYDRWASAGCDGWAWDD
VLPYFRRAECNERVAGRDDDPWHGGSGPLHVSDLRTPNPFGQRFIEAAQQAGYRRNDDFN
GVDQEGAGWYQVTQHGGERWNAARAYLHRGDAADHASNGGRENLAVMTGTQVLRIAFEGR
HAVGVVVRLDGRETLLRARREVIVSAGAFGSPQLLMVSGVGPASHLRELGIPVVHDLPGV
GANLQDHLDIIVHKRVAASELFGVSVGGAFRLLAEILRYRRERTGMLTSNFAEAGAFLKS
HAGLDEPDLQLHFVVGMADDHMRRIRLGHGYSCHVCVLRPKSRGEVRLASPDIREAPMID
PCFLSDPRDLDGLVAGVRIVRRILAQPQLAAFGGKELYSAHLPADGGDDSAIRAMIRAHA
DTIYHPVGTCRMGMDEYAVVDPQLRVRGVEGLRVVDASIMPTLIGGNTNAPAIMIGERAH
DLIRHGPRVVLRMAEVLAV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory