SitesBLAST
Comparing RR42_RS01580 FitnessBrowser__Cup4G11:RR42_RS01580 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
60% identity, 96% coverage: 9:492/505 of query aligns to 1:484/489 of 4zz7A
- active site: N149 (= N158), K172 (= K181), L246 (= L255), C280 (= C289), E382 (= E390), A462 (= A470)
- binding nicotinamide-adenine-dinucleotide: T146 (= T155), P147 (= P156), F148 (= F157), N149 (= N158), K172 (= K181), E175 (= E184), K205 (= K214), V208 (= V217), F222 (= F231), V223 (= V232), G224 (= G233), S225 (= S234), I228 (= I237), L246 (= L255), G247 (= G256), C280 (= C289), E382 (= E390), F384 (= F392)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
61% identity, 95% coverage: 12:491/505 of query aligns to 3:457/468 of 5tjrD
- active site: N144 (= N158), K167 (= K181), L241 (= L255), C270 (= C289), E356 (= E390), A436 (= A470)
- binding adenosine-5'-diphosphate: I140 (= I154), T141 (= T155), F143 (= F157), K167 (= K181), E170 (= E184), K200 (= K214), F217 (= F231), S220 (= S234), I223 (= I237)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
56% identity, 96% coverage: 11:493/505 of query aligns to 6:489/491 of 4iymC
- active site: N153 (= N158), K176 (= K181), F250 (≠ L255), C284 (= C289), E386 (= E390), Q466 (≠ A470)
- binding nicotinamide-adenine-dinucleotide: I149 (= I154), T150 (= T155), P151 (= P156), F152 (= F157), N153 (= N158), F154 (= F159), K176 (= K181), K209 (= K214), V212 (= V217), F226 (= F231), V227 (= V232), G228 (= G233), S229 (= S234), I232 (= I237), G251 (= G256), C284 (= C289), E386 (= E390), F388 (= F392)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
48% identity, 96% coverage: 8:490/505 of query aligns to 3:482/487 of P42412
- C36 (≠ G41) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R112) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T155) binding NAD(+)
- F152 (= F157) binding NAD(+)
- C160 (≠ M165) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K181) binding NAD(+)
- E179 (= E184) binding NAD(+)
- R180 (= R185) binding NAD(+)
- S229 (= S234) binding NAD(+)
- T251 (≠ G256) binding NAD(+)
- R283 (= R288) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I292) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ T356) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E390) binding NAD(+)
- C413 (≠ S421) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
48% identity, 96% coverage: 8:490/505 of query aligns to 1:480/484 of 1t90A
- active site: N151 (= N158), K174 (= K181), L248 (= L255), C282 (= C289), E380 (= E390), A460 (= A470)
- binding nicotinamide-adenine-dinucleotide: I147 (= I154), A148 (≠ T155), P149 (= P156), F150 (= F157), N151 (= N158), W159 (= W166), K174 (= K181), E177 (= E184), R178 (= R185), H207 (≠ K214), V225 (= V232), G226 (= G233), S227 (= S234), V230 (≠ I237), L248 (= L255), T249 (≠ G256), C282 (= C289), E380 (= E390), F382 (= F392)
2d4eC Crystal structure of the hpcc from thermus thermophilus hb8
34% identity, 93% coverage: 14:485/505 of query aligns to 29:501/515 of 2d4eC
- active site: N173 (= N158), K196 (= K181), E271 (≠ L255), C305 (= C289), E409 (= E390), E486 (≠ A470)
- binding nicotinamide-adenine-dinucleotide: I169 (= I154), T170 (= T155), P171 (= P156), W172 (≠ F157), K196 (= K181), A198 (≠ S183), G229 (= G213), G233 (≠ V217), A234 (≠ D218), T248 (≠ V232), G249 (= G233), E250 (≠ S234), T253 (≠ I237), E271 (≠ L255), L272 (≠ G256), C305 (= C289), E409 (= E390), F411 (= F392), F475 (= F457)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
34% identity, 95% coverage: 8:485/505 of query aligns to 1:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
34% identity, 95% coverage: 8:485/505 of query aligns to 1:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
34% identity, 95% coverage: 8:485/505 of query aligns to 1:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
34% identity, 95% coverage: 8:485/505 of query aligns to 1:477/488 of 8vqwC