SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS01600 FitnessBrowser__Cup4G11:RR42_RS01600 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q1LRY0 Fused isobutyryl-CoA mutase; EC 5.4.99.13; EC 3.6.5.- from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34) (Ralstonia metallidurans) (see 2 papers)
87% identity, 100% coverage: 1:1102/1102 of query aligns to 1:1093/1093 of Q1LRY0

query
sites
Q1LRY0

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M

H39 (= H45) binding axial binding residue
169:417 (vs. 175:423, 87% identical) GTPase chaperone MeaI
GAGKSS 219:224 (= GAGKSS 225:230) binding GTP
S223 (= S229) binding Mg(2+)
I248 (= I254) binding Mg(2+)
D249 (= D255) binding Mg(2+)
D262 (= D268) binding Mg(2+); binding Mg(2+)
R265 (= R271) binding GTP
E310 (= E316) binding Mg(2+); binding Mg(2+)
T311 (= T317) binding Mg(2+)
NKFD 357:360 (= NKFD 363:366) binding GTP
418:579 (vs. 424:588, 75% identical) Linker
F587 (= F596) binding substrate
F598 (= F607) mutation to A: Switches the substrate specificity and enhances the catalytic efficiency of the isovaleryl-CoA mutase over the native isobutyryl-CoA mutase activity about 4000-fold. Is even more susceptible to inactivation than wild-type during turnover.
R622 (= R631) binding substrate
R728 (= R737) binding substrate
Y772 (= Y781) binding substrate
S821 (= S830) binding substrate
R856 (= R865) binding substrate
K861 (= K870) binding substrate
E973 (= E982) binding GTP
N1092 (= N1101) binding GTP

8sslA Isobutyryl-coa mutase fused q341a in the presence of gtp (see paper)
88% identity, 98% coverage: 28:1102/1102 of query aligns to 1:1072/1072 of 8sslA

query
sites
8sslA

G

G

P

P

V

A

N

N

K

K

V

V

R

R

F

F

V

V

T

T

A

A

A

A

S

S

L

L

F

F

D

D

G

G

H

H

D

D

A

A

S

S

I

I

N

N

I

I

M

M

R

R

R

R

I

I

L

L

Q

Q

S

S

S

Q

G

G

C

C

E

E

V

V

I

I

H

H

L

L

G

G

H

H

N

N

R

R

S

S

V

V

E

Q

E

E

V

V

V

V

S

T

A

A

A

A

L

L

Q

Q

E

E

D

D

V

V

Q

Q

G

G

I

I

A

A

I

I

S

S

S

S

Y

Y

Q

Q

G

G

G

G

H

H

V

V

E

E

Y

Y

F

F

K

K

Y

Y

M

M

V

I

D

D

L

L

L

L

R

R

R

E

R

H

G

G

G

G

E

E

H

H

I

I

Q

Q

L

V

F

F

G

G

G

G

G

G

G

G

G

G

V

V

I

I

V

V

A

P

D

D

E

E

I

I

R

R

E

E

L

L

Q

Q

A

A

Y

Y

G

G

V

V

A

A

R

R

I

I

F

Y

S

S

P

P

E

E

D

D

G

G

Q

Q

R

R

L

M

G

G

L

L

A

A

G

G

M

M

I

I

A

T

D

D

M

M

V

A

Q

Q

R

R

C

C

D

D

I

I

D

D

L

L

A

T

R

R

Y

Y

A

A

P

P

A

T

T

T

L

L

D

D

A

T

M

V

K

V

G

A

G

G

D

D

R

R

R

R

A

A

L

L

A

A

Q

Q

L

L

I

I

T

T

A

A

L

L

E

E

N

N

G

G

K

K

A

A

D

D

P

P

A

E

L

L

A

V

D

S

T

A

L

L

R

H

A

A

E

Q

A

A

A

K

T

A

V

A

K

A

T

V

P

P

V

V

L

L

G

G

I

I

T

T

G

G

T

T

G

G

G

G

A

A

G
|
G

K
|
K

S
|
S

S

S

L

L

T

T

D

D

E

E

L

L

I

I

R

R

R

R

I

F

R

R

L

L

D

D

Q

Q

R

D

D

D

A

A

L

L

S

S

I

I

A

A

V

V

I

I

S

S

I

I

D

D

P

P

S

S

R

R

R

R

K

K

S

S

G

G

G

G

A

A

L

L

L

L

G

G

D
|
D

R

R

I

I

R
|
R

M

M

N

N

A

A

I

I

N

N

H

H

P

P

H

N

I

I

F

F

M

M

R

R

S

S

M

L

A

A

T

T

R

R

E

E

A

A

G

G

S

S

E

E

I

I

S

S

R

Q

A

A

L

L

P

P

D

D

V

V

L

I

A

A

A

A

C

C

K

K

V

A

A

A

G

R

F

F

D

D

L

L

I

V

I

I

V

V

E

E

T

T

S

S

G

G

I

I

G

G

Q

Q

G

G

D

D

A

A

A

A

I

I

V

V

A

P

H

H

V

V

D

D

L

L

S

S

L

L

Y

Y

V

V

M

M

T

T

P

P

E

E

F

F

G

G

A

A

A

A

S

S

Q

A

L

L

E

E

K

K

I

I

D

D

M

M

L

L

D

D

F

F

A

A

D

D

F

F

V

V

A

A

I

I

N

N

K
|
K

F

F

D
|
D

R

R

K

K

G

G

A

A

Q

Q

D

D

A

A

W

W

R

R

D

D

V

V

A

A

K

K

Q

Q

V

V

Q

Q

R

R

N

N

R

R

E

E

Q

Q

W

W

H

H

A

S

K

R

P

A

E

E

E

D

M

M

P

P

V

V

Y

Y

G

G

T

T

Q
|
Q

A
|
A

S
|
S

R

R

F

F

N

N

D

D

D

D

G

G

V

V

T

T

M

M

L

L

Y

Y

Q

Q

G

G

L

L

R

V

E

G

A

A

L

L

C

G

A

A

R

R

G

G

M

M

R

S

A

L

P

K

A

P

G

G

V

T

L

L

P

P

Q

N

L

L

S

E

G

G

R

R

V

I

S

S

T

T

G

G

Q

Q

N

N

V

V

I

I

V

V

P

P

P

P

A

A

R

R

N

S

R

R

Y

Y

L

L

A

A

E

E

I

L

A

A

D

D

T

T

V

V

R

R

A

A

Y

Y

H

H

R

R

R

R

V

V

D

V

A

A

Q

Q

S

S

R

K

L

L

A

A

R

R

E

E

R

R

Q

Q

Q

Q

L

L

R

R

E

A

A

A

R

H

R

D

M

M

L

L

L

-

A

-

A

-

S

Q

G

G

Q

A

E

G

D

H

Q

E

G

S

E

A

A

A

L

L

Q

E

A

T

L

L

A

A

T

S

E

E

R

R

D

D

A

V

A

S

L

L

G

G

A

A

Q

V

E

E

R

R

K

K

L

L

L

L

A

A

M

M

W

W

P

P

Q

Q

M

M

R

Q

K

Q

A

A

Y

Y

S

S

G

G

D

D

E

E

Y

Y

V

V

V

V

K

K

I

I

R

R

D

D

K

K

E

E

I

I

R

R

T

T

T

G

L

L

T

I

T

S

T

T

T

T

L

L

S

S

G

G

T

T

T

K

V

I

R

R

K

K

V

V

V

V

L

L

P

P

R

R

F

F

E

E

D

D

D

E

G

G

E

E

V

I

L

L

K

K

W

W

L

L

L

M

R

R

E

E

N

N

V

V

P

P

G

G

S

S

F

F

P

P

Y

Y

T

T

A

A

G

G

V

V

F

F

A

A

F

F

K

K

R

R

E

E

N

G

E

E

D

D

P

P

T

T

R

R

M

M

F

F

A

A

G

G

E

E

G

G

D

D

A

A

F

F

R

R

T

T

N

N

R

R

R

R

F

F

K

K

L

L

V

V

S

S

E

E

G

G

M

M

D

E

A

A

K

K

R

R

L

L

S

S

T

T

A

A

F

F

D

D

S

S

V

V

T

T

L

L

Y

Y

G

G

E

E

D

D

P

P

H

H

T

E

R

R

P

P

D

D

I

I

Y

Y

G

G

K

K

V

V

G

G

N

N

S

S

G

G

V

V

S

S

I

I

A

A

T

T

L

L

D

E

D

D

M

M

K

K

V

V

L

L

Y

Y

D

D

G

G

F

F

D

D

L

L

T

T

S

N

P

P

S

S

T

T

S

S

V

V

S

S

M

M

T

T

I

I

N

N

G

G

P

P

A

A

P

P

T

T

I

I

L

L

A

A

M

M

F

F

M

M

N

N

T

T

A

A

I

I

D

D

Q

Q

Q

Q

F

I

D

D

K

R

F

F

R

R

A

A

D

D

N

N

D

G

R

R

E

D

P

P

T

T

V

A

D

D

E

E

Q

E

G

A

K

K

I

I

R

R

A

A

W

W

V

V

L

L

Q

Q

N

N

V

V

R

R

G

G

T

T

V

V

Q

Q

A

A

D

D

I

I

L

L

K

K

E

E

D

D

Q

Q

G

G

Q

Q

N

N

T

T

C

C

I

I

F

F

S

S

T

T

E

E

F

F

S

S

L

L

K

K

V

V

M

M

G

G

D

D

I

I

Q

Q

A

E

Y

Y

F

F

V

V

H

H

H

H

Q

Q

V

V

R

R

N

N

F

F

Y

Y

S

S

V

V

S

S

I

I

S

S

G

G

Y

Y

H

H

I

I

A

A

E

E

A

A

G

G

A

A

N

N

P

P

I

I

S

S

Q

Q

L

L

A

A

F

F

T

T

L

L

S

A

N

N

G

G

F

F

T

T

Y

Y

V

V

E

E

A

A

Y

Y

L

L

A

A

R

R

G

G

M

M

H

H

I

I

D

D

D

D

F

F

A

A

P

P

N

N

L

L

S

S

F

F

F

F

F

F

S

S

N

N

G

G

M

M

D

D

P

P

E

E

Y

Y

S

S

V

V

L

L

G

G

R

R

V

V

A

A

R

R

R

R

I

I

W

W

A

A

V

V

T

T

L

M

R

R

D

D

K

K

Y

Y

G

G

A

A

N

N

E

D

R

R

S

S

Q

Q

K

K

L

L

K

K

Y

Y

H

H

I

I

Q

Q

T

T

S

S

G

G

R

R

S

S

L

L

H

H

A

A

Q

Q

E

E

I

I

D

D

F

F

N

N

D

D

I

I

R

R

T

T

T

T

L

L

Q

Q

A

A

L

L

I

I

A

A

I

I

Y

Y

D

D

N

N

C

C

N

N

S

S

L

L

H

H

T

T

N

N

A

A

Y

Y

D

D

E

E

A

A

I

I

T

T

T

T

P

P

T

T

G

A

E

E

S

S

V

V

R

R

R

R

A

A

L

L

A

A

I

I

Q

Q

L

L

I

I

I

I

N

N

R

R

E

E

W

W

G

G

V

V

A

A

R

K

C

C

E

E

N

N

P

P

N

N

Q

Q

G

G

S

S

F

F

L

L

I

I

D

E

E

E

L

L

T

T

D

D

L

L

V

V

E

E

D

E

A

A

V

V

M

L

Q

Q

E

E

F

F

E

E

R

R

I

I

A

A

E

E

R

R

G

G

G

G

V

V

L

L

G

G

A

A

M

M

E

E

T

T

G

G

Y

Y

Q

Q

R

R

G

G

K

K

I

I

Q

Q

D

E

E

E

S

S

L

L

Y

Y

Y

Y

E

E

Q

Q

R

L

K

K

H

H

D

D

G

G

T

T

L

L

P

P

I

I

I

I

G

G

V

V

N

N

T

T

F

F

R

R

N

N

P

P

D

N

G

G

D

D

A

P

A

T

P

P

P

Q

S

T

L

L

E

E

L

L

A

A

R

R

S

S

S

S

E

E

E

D

E

E

K

K

Q

Q

G

S

Q

Q

L

L

A

H

R

R

L

L

A

T

D

E

F

F

H

H

A

G

R

A

H

H

A

Q

D

A

A

D

A

A

P

E

A

A

M

M

L

L

Q

A

R

R

L

L

R

R

Q

Q

A

A

V

V

I

I

D

D

N

N

G

R

N

N

V

V

F

F

A

A

V

V

L

L

M

M

D

D

A

A

V

V

R

R

V

V

C

C

S

S

L

L

G

G

Q

Q

V

I

T

T

H

H

A

A

L

L

F

F

E

E

V

V

G

G

G

G

Q

Q

Y

Y

R

R

R

R

N
|
N

M

M

binding guanosine-5'-diphosphate: G200 (= G227), K201 (= K228), S202 (= S229), D241 (= D268), R244 (= R271), K337 (= K364), D339 (= D366), Q374 (= Q401), A375 (= A402), S376 (= S403), N1071 (= N1101)
binding magnesium ion: K201 (= K228), D241 (= D268)

4xc6A Isobutyryl-coa mutase fused with bound adenosylcobalamin, gdp, and mg (holo-icmf/gdp) (see paper)
87% identity, 98% coverage: 27:1102/1102 of query aligns to 1:1067/1067 of 4xc6A

query
sites
4xc6A

K

R

G

G

P

P

V

A

N

N

K
|
K

V

V

R

R

F

F

V

V

T

T

A

A

A

A

S

S

L

L

F

F

D

D

G
|
G

H
|
H

D
|
D

A
|
A

S
|
S

I

I

N

N

I

I

M
|
M

R

R

R

R

I

I

L

L

Q

Q

S

S

S

Q

G

G

C

C

E

E

V

V

I

I

H

H

L

L

G

G

H

H

N

N

R

R

S

S

V

V

E

Q

E

E

V

V

V

V

S

T

A

A

A

A

L

L

Q

Q

E

E

D

D

V

V

Q

Q

G

G

I

I

A

A

I

I

S

S

S

S

Y
|
Y

Q
|
Q

G

G

G

G

H

H

V

V

E

E

Y

Y

F

F

K

K

Y

Y

M

M

V

I

D

D

L

L

L

L

R

R

R

E

R

H

G

G

G

G

E

E

H

H

I

I

Q

Q

L

V

F

F

G

G

G
|
G

G

G

G
|
G

G

G

V
|
V

I

I

V

V

A

P

D

D

E

E

I

I

R

R

E

E

L

L

Q

Q

A

A

Y

Y

G

G

V

V

A

A

R

R

I

I

F
x
Y

S
|
S

P
|
P

E

E

D

D

G

G

Q

Q

R

R

L

M

G

G

L

L

A

A

G

G

M
|
M

I

I

A

T

D

D

M

M

V

A

Q

Q

R

R

C

C

D

D

I

I

D

D

L

L

A

T

R

R

Y

Y

A

A

P

P

A

T

T

T

L

L

D

D

A

T

M

V

K

V

G

A

G

G

D

D

R

R

R

R

A

A

L

L

A

A

Q

Q

L

L

I

I

T

T

A

A

L

L

E

E

N

N

G

G

K

K

A

A

D

D

P

P

A

E

L

L

A

V

D

S

T

A

L

L

R

H

A

A

E

Q

A

A

A

K

T

A

V

A

K

A

T

V

P

P

V

V

L

L

G

G

I

I

T

T

G

G

T

T

G

G

G
|
G

A

A

G
|
G

K
|
K

S
|
S

S
|
S

L

L

T

T

D

D

E

E

L

L

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V

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|
D

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K

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G

G

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G

A

A

L

L

L

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D
|
D

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R

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I

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|
R

M

M

N

N

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|
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|
N

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|
F

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|
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|
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Y
|
Y

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|
H

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|
E

A
|
A

G

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N

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F

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T

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N

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F

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Y

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Y

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L

A

A

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R

G

G

M

M

H

H

I

I

D

D

D

D

F

F

A

A

P

P

N

N

L

L

S

S

F

F

F

F

F

F

S

S

N

N

G

G

M

M

D

D

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P

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L

L

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G

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A

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I

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W

A

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V

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M

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D

D

K

K

Y

Y

G

G

A

A

N

N

E

D

R

R

S

S

Q

Q

K

K

L

L

K

K

Y

Y

H

H

I

I

Q

Q

T

T

S

S

G
|
G

R
|
R

S

S

L

L

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T

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L

L

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D

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C

C

N

N

S

S

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L

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H

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N

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Y

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D

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|
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|
T

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P

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A

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V

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L

A

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I

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L

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N

N

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R

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E

W

W

G

G

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V

A

A

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K

C

C

E

E

N

N

P

P

N

N

Q

Q

G

G

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F

F

L

L

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M

E
|
E

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T

G

G

Y

Y

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Q

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R

G

G

K

K

I

I

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D

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S

L

L

Y

Y

Y

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Q

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K

H
|
H

D

D

G

G

T

T

L

L

P

P

I

I

I

I

G

G

V

V

N

N

T

T

F

F

R

R

N

N

P

P

D

N

G

G

D

D

A

P

A

T

P

P

P

Q

S

T

L

L

E

E

L

L

A

A

R

R

S

S

S

S

E

E

E

D

E

E

K

K

Q

Q

G

S

Q

Q

L

L

A

H

R

R

L

L

A

T

D

E

F

F

H

H

A

G

R

A

H

H

A

Q

D

A

A

D

A

A

P

E

A

A

M

M

L

L

Q

A

R

R

L

L

R

R

Q

Q

A

A

V

V

I

I

D

D

N

N

G

R

N

N

V

V

F

F

A

A

V

V

L

L

M

M

D

D

A

A

V

V

R

R

V

V

C

C

S

S

L

L

G

G

Q

Q

V

I

T

T

H

H

A

A

L

L

F

F

E

E

V

V

G

G

G

G

Q

Q

Y

Y

R

R

R

R

N

N

M

M

active site: K6 (= K32), F572 (= F607), Y753 (= Y788), H754 (= H789)
binding cobalamin: G18 (= G44), H19 (= H45), D20 (= D46), A21 (= A47), S22 (= S48), M26 (= M52), Y66 (= Y92), Q67 (= Q93), G94 (= G120), G96 (= G122), V98 (= V124), Y116 (≠ F142), S117 (= S143), P118 (= P144), M129 (= M155), F601 (= F636), L606 (= L641), S624 (= S659), Q716 (= Q751), H754 (= H789), E757 (= E792), A758 (= A793), G842 (= G877), R843 (= R878), E879 (= E914), A880 (= A915), T882 (= T917), H967 (= H1002)
binding guanosine-5'-diphosphate: G199 (= G225), G201 (= G227), K202 (= K228), S203 (= S229), S204 (= S230), R245 (= R271), N337 (= N363), K338 (= K364), D340 (= D366), Q375 (= Q401), S377 (= S403), E947 (= E982)
binding magnesium ion: S203 (= S229), D229 (= D255), D242 (= D268), D242 (= D268), E290 (= E316), E290 (= E316)

5cjwA Isobutyryl-coa mutase fused with bound adenosylcobalamin, gdp, mg (holo-icmf/gdp), and substrate pivalyl-coenzyme a (see paper)
87% identity, 98% coverage: 27:1102/1102 of query aligns to 1:1063/1063 of 5cjwA

query
sites
5cjwA

K

R

G

G

P

P

V

A

N

N

K
|
K

V

V

R

R

F

F

V

V

T

T

A

A

A

A

S

S

L

L

F

F

D

D

G
|
G

H
|
H

D
|
D

A
|
A

S
|
S

I

I

N

N

I

I

M
|
M

R

R

R

R

I

I

L

L

Q

Q

S

S

S

Q

G

G

C

C

E

E

V

V

I

I

H

H

L

L

G

G

H

H

N

N

R

R

S

S

V

V

E

Q

E

E

V

V

V

V

S

T

A

A

A

A

L

L

Q

Q

E

E

D

D

V

V

Q

Q

G

G

I

I

A

A

I

I

S

S

S

S

Y
|
Y

Q
|
Q

G

G

G

G

H

H

V

V

E

E

Y

Y

F

F

K

K

Y

Y

M

M

V

I

D

D

L

L

L

L

R

R

R

E

R

H

G

G

G

G

E

E

H

H

I

I

Q

Q

L

V

F

F

G

G

G
|
G

G

G

G
|
G

G

G

V
|
V

I

I

V

V

A

P

D

D

E

E

I

I

R

R

E

E

L

L

Q

Q

A

A

Y

Y

G

G

V

V

A

A

R

R

I

I

F
x
Y

S
|
S

P
|
P

E

E

D

D

G

G

Q

Q

R

R

L

M

G

G

L

L

A

A

G

G

M

M

I

I

A

T

D

D

M

M

V

A

Q

Q

R

R

C

C

D

D

I

I

D

D

L

L

A

T

R

R

Y

Y

A

A

P

P

A

T

T

T

L

L

D

D

A

T

M

V

K

V

G

A

G

G

D

D

R

R

R

R

A

A

L

L

A

A

Q

Q

L

L

I

I

T

T

A

A

L

L

E

E

N

N

G

G

K

K

A

A

D

D

P

P

A

E

L

L

A

V

D

S

T

A

L

L

R

H

A

A

E

Q

A

A

A

K

T

A

V

A

K

A

T

V

P

P

V

V

L

L

G

G

I

I

T

T

G

G

T

T

G

G

G
|
G

A

A

G
|
G

K
|
K

S
|
S

S
|
S

L

L

T

T

D

D

E

E

L

L

I

I

R

R

R

R

I

F

R

R

L

L

D

D

Q

Q

R

D

D

D

A

A

L

L

S

S

I

I

A

A

V

V

I

I

S

S

I

I

D
|
D

P

P

S

S

R

R

R

R

K

K

S

S

G

G

G

G

A

A

L

L

L

L

G

G

D
|
D

R

R

I

I

R
|
R

M

M

N

N

A

A

I

I

N

N

H

H

P

P

H

N

I

I

F

F

M

M

R

R

S

S

M

L

A

A

T

T

R

R

E

E

A

A

G

G

S

S

E

E

I

I

S

S

R

Q

A

A

L

L

P

P

D

D

V

V

L

I

A

A

A

A

C

C

K

K

V

A

A

A

G

R

F

F

D

D

L

L

I

V

I

I

V

V

E
|
E

T

T

S

S

G

G

I

I

G

G

Q

Q

G

G

D

D

A

A

A

A

I

I

V

V

A

P

H

H

V

V

D

D

L

L

S

S

L

L

Y

Y

V

V

M

M

T

T

P

P

E

E

F

F

G

G

A

A

A

A

S

S

Q

Q

L

L

E

E

K

K

I

I

D

D

M

M

L

L

D

D

F

F

A

A

D

D

F

F

V

V

A

A

I

I

N
|
N

K
|
K

F

F

D
|
D

R

R

K

K

G

G

A

A

Q

Q

D

D

A

A

W

W

R

R

D

D

V

V

A

A

K

K

Q

Q

V

V

Q

Q

R

R

N

N

R

R

E

E

Q

Q

W

W

H

H

A

S

K

R

P

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