SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS01815 FitnessBrowser__Cup4G11:RR42_RS01815 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
49% identity, 97% coverage: 1:247/255 of query aligns to 4:248/249 of P12282

query
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P12282

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R14 (= R11) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A41) mutation to A: No effect.
R73 (= R70) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (= C125) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D127) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C139) mutation to A: No effect.
C172 (= C170) mutation to A: No zinc bound and no enzyme activity.
C175 (= C173) mutation to A: No zinc bound and no enzyme activity.
C187 (= C186) mutation to A: No effect.
C231 (≠ M230) mutation to A: No effect.
C244 (= C243) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C246) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
49% identity, 97% coverage: 1:247/255 of query aligns to 3:240/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R11), D129 (= D127)
binding adenosine monophosphate: G37 (= G35), G39 (= G37), G40 (= G38), D61 (= D59), F62 (≠ D60), K85 (= K83), L108 (≠ R106), C127 (= C125), T128 (≠ C126), D129 (= D127), N130 (= N128), V133 (≠ T131)
binding zinc ion: C171 (= C170), C236 (= C243), C239 (= C246)

1jw9B Structure of the native moeb-moad protein complex (see paper)
49% identity, 97% coverage: 1:247/255 of query aligns to 3:240/240 of 1jw9B

query
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1jw9B

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active site: R13 (= R11), D129 (= D127)
binding zinc ion: C171 (= C170), C236 (= C243), C239 (= C246)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
46% identity, 98% coverage: 1:251/255 of query aligns to 1:248/251 of P30138

query
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P30138

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C169 (= C170) binding Zn(2+)
C172 (= C173) binding Zn(2+)
W174 (≠ F175) mutation to A: No adenylation of ThiS.
C184 (= C186) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C243) binding Zn(2+)
C243 (= C246) binding Zn(2+)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
45% identity, 94% coverage: 1:239/255 of query aligns to 3:217/217 of 1jwaB

query
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1jwaB

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T

S

V

L

Q

Q

A

A

A

M

E

E

A

A

L

I

K

K

L

M

L

L

M

A

G

G

V

Y

G

G

T

K

T

P

L

A

A

S

G

G

R

K

L

I

L

V

M

M

V

Y

D

D

A

A

L

M

G

T

M

C

E

Q

W

F

T

R

T

E

M

M

R

K

L

L

A

M

R

R

active site: R13 (= R11), D129 (= D127)
binding adenosine-5'-triphosphate: G39 (= G37), G40 (= G38), D61 (= D59), F62 (≠ D60), R72 (= R70), K85 (= K83), L108 (≠ R106), D129 (= D127), N130 (= N128), V133 (≠ T131)

1zfnA Structural analysis of escherichia coli thif (see paper)
46% identity, 97% coverage: 1:247/255 of query aligns to 1:244/244 of 1zfnA

query
sites
1zfnA

M

M

N

N

D

D

D

R

Q

D

L

F

L

M

R

R

Y

Y

S

S

R
|
R

H

Q

I

I

L

L

D

D

E

D

L

I

G

A

I

L

E

D

G

G

Q

Q

S

Q

R

K

L

L

A

D

S

S

H

Q

A

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

G

G

A

T

A

P

A

A

L

A

P

L

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

T

I

L

T

V

V

L

V

A

D
|
D

D

D

V

D

V

V

D

H

L

L

T

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

T

T

A

E

N

D

V

I

G

D

R

R

P

P

K
|
K

V

S

E

Q

S

V

A

S

R

Q

E

Q

G

R

M

L

L

T

R

Q

I

L

N

N

P

P

G

D

I

I

D

Q

V

L

R

T

L

A

V

L

Q

Q

A

Q

R

R

V

L

G

T

A

G

A

E

E

A

L

L

A

K

H

D

L

A

V

V

A

A

D

R

A

A

D

D

V

V

L

L

D

D

C

C

C
x
T

D
|
D

N

N

F

M

D

A

T
|
T

R

R

Q

Q

A

E

V

I

N

N

R

A

A

A

C

C

V

V

Q

A

H

L

S

N

V

T

P

P

L

L

V

I

S

T

G

A

A

S

A

A

L

V

R

G

F

F

D

G

G

G

Q

Q

I

L

S

M

V

V

F

L

D

T

H

P

R

P

Q

W

P

E

G

Q

A

G

P

-

C
|
C

Y

Y

A

R

C
|
C

L

L

F

W

P

P

P

D

S

N

Q

Q

P

E

A

P

Q

-

E

E

V

R

A

N

C

C

A

R

T

T

M

A

G

G

V

V

F

V

A

G

P

P

L

V

V

V

G

G

M

V

V

M

G

G

T

T

V

L

Q

Q

A

A

A

L

E

E

A

A

L

I

K

K

L

L

M

S

G

G

V

I

G

E

T

T

T

P

L

-

A

A

G

G

R

E

L

L

L

R

M

L

V

F

D

D

A

G

L

K

G

S

M

S

E

Q

W

W

T

R

T

S

M

L

R

A

L

L

A

R

R

R

V

A

A

S

D

G

C
|
C

E

P

V

V

C
|
C

G

G

active site: R11 (= R11), D127 (= D127)
binding adenosine-5'-triphosphate: I34 (= I34), G35 (= G35), G37 (= G37), G38 (= G38), D59 (= D59), R70 (= R70), Q71 (= Q71), K83 (= K83), T126 (≠ C126), D127 (= D127), T131 (= T131)
binding zinc ion: C169 (= C170), C172 (= C173), C240 (= C243), C243 (= C246)

1zud3 Structure of this-thif protein complex (see paper)
46% identity, 97% coverage: 1:248/255 of query aligns to 1:240/240 of 1zud3

query
sites
1zud3

M

M

N

N

D

D

D

R

Q

D

L

F

L

M

R

R

Y

Y

S

S

R
|
R

H

Q

I

I

L
|
L

L

L

D
|
D

E

D

L

I

G

A

I

L

E

D

G

G

Q

Q

S

Q

R

K

L

L

A

D

S

S

H

Q

A

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

A

P

A

A

L

A

P

L

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

T

I

L

T

V

V

L

V

A

D

D

V

D

V

V

D

H

L

L

T

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

T

T

A

E

N

D

V

I

G

D

R

R

P

P

K

K

V

S

E

Q

S

V

A

S

R

Q

E

Q

G

R

M

L

L

T

R

Q

I

L

N

N

P

P

G

D

I

I

D

Q

V

L

R

T

L

A

V

L

Q

Q

A

Q

R

R

V

L

G

T

A

G

A

E

E

A

L

L

A

K

H

D

L

A

V

V

A

A

D

R

A

A

D

D

V

V

L

L

D

D

C

C

C

T

D
|
D

N

N

F

M

D

A

T

T

R

R

Q

Q

A

E

V

I

N

N

R

A

A

A

C

C

V

V

Q

A

H

L

S

N

V

T

P

P

L

L

V

I

S

T

G

A

A

S

A

A

L

V

R

G

F

F

D

G

G

G

Q

Q

I

L

S

M

V

V

F

L

D

T

H

P

R

P

Q

W

P

E

G

Q

A

G

P

-

C
|
C

Y

Y

A

R

C

C

L

L

F

W

P

P

P

D

S

N

Q

Q

P

E

A

-

Q

-

E

-

V

-

A

-

C

-

A

P

T

T

M

A

G

G

V

V

F

V

A

G

P

P

L

V

V

V

G

G

M

V

V

M

G

G

T

T

V

L

Q

Q

A

A

A

L

E

E

A

A

L

I

K

K

L

L

M

S

G

G

V

I

G

E

T

T

T

P

L

-

A

A

G

G

R

E

L

L

L

R

M

L

V

F

D

D

A

G

L

K

G

S

M

S

E

Q

W

W

T

R

T

S

M

L

R

A

L

L

A

R

R

R

V

A

A

S

D

G

C
|
C

E

P

V

V

C
|
C

G

G

active site: R11 (= R11), D127 (= D127)
binding calcium ion: L14 (= L14), D16 (= D16)
binding zinc ion: C169 (= C170), C235 (= C243), C238 (= C246)

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
50% identity, 95% coverage: 5:247/255 of query aligns to 9:249/270 of D4GSF3

query
sites
D4GSF3

Q

Q

L

L

L

D

R

R

Y

Y

S

S

R

R

H

H

I

I

L

I

L

M

D

D

E

E

L

V

G

G

I

P

E

E

G

G

Q

Q

S

G

R

R

L

L

A

S

S

S

H

R

A

V

L

V

V

V

I

V

G

G

A

A

G

G

L

L

G

G

A

A

A

P

A

A

L

I

P

Q

Y

Y

L

L

A

A

S

A

A

V

G

G

V

V

G

G

T

E

I

L

T

V

V

V

D

D

V

V

D

E

L

R

T

S

N

N

L

L

Q

Q

R

R

Q

Q

V

V

I

V

H

H

T

C

T

D

A

D

N

D

V

V

G

G

R

T

P

P

K

K

V

A

E

E

S

S

A

A

R

A

E

A

G

F

M

V

L

R

R

G

I

L

N

N

P

P

G

D

I

V

D

S

V

V

R

E

L

P

V

V

Q

E

A

A

R

R

V

V

G

D

A

K

A

S

E

N

L

V

A

H

H

E

L

V

V

V

A

A

D

G

A

S

D

D

V

V

L

V

D

D

C

A

C

S

D

D

N

N

F

F

D

P

T

T

R

R

Q

Y

A

L

V

L

N

N

R

D

A

V

C

C

V

R

Q

F

H

E

S

G

V

I

P

P

L

L

V

V

S

H

G

G

A

A

A

I

L

Y

R

K

F

F

D

E

G

G

Q

Q

I

A

S

T

V

T

F

L

D

-

H

-

R

-

Q

V

P

P

G

D

A

G

P

P

C

C

Y

Y

A

R

C

C

L

L

F

F

P

P

P

E

S

A

-

P

Q

E

P

P

A

G

Q

T

E

V

V

P

A

D

C
|
C

A

A

T

T

M

T

G

G

V

V

F

L

A

G

P

V

L

L

V

P

G

G

M

T

V

V

G

G

T

C

V

I

Q

Q

A

A

A

T

E

E

A

A

L

M

K

K

L

L

M

L

G

D

V

E

G

G

T

E

T

A

L

L

A

D

G

G

R

R

L

L

M

F

V

Y

D

D

A

A

L

M

G

D

M

M

E

T

W

F

T

E

T

T

M

V

R

P

L

Y

A

R

R

T

V

N

A

P

D

D

C

C

E

P

V

V

C

C

G

G

C188 (= C186) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 97% coverage: 1:247/255 of query aligns to 64:310/453 of Q9VLJ8

query
sites
Q9VLJ8

M

L

N

T

D

N

D

D

Q

D

L

I

L

A

R

R

Y

Y

S

S

R

R

H

Q

I

L

L

I

L

L

D

P

E

D

L

F

G

G

I

V

E

Q

G

G

Q

Q

S

L

R

K

L

L

L

K

A

N

S

S

H

S

A

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A

C

A

P

A

A

L

A

P

Q

Y

Y

L

L

A

A

S

A

A

A

G

G

V

C

G

G

T

H

I

L

T

G

V

L

V

V

D

D

D

Y

D

D

V

E

V

V

D

E

L

R

T

S

N

N

L

F

Q

H

R

R

Q

Q

V

I

I

L

H

H

T

S

T

E

A

D

N

R

V

C

G

G

R

M

P

S

K

K

V

A

E

E

S

S

A

A

R

R

E

I

G

A

M

L

L

L

R

E

I

L

N

N

P

P

G

H

I

C

D

E

V

I

R

Q

L

C

V

H

Q

S

A

R

R

M

V

L

G

Y

A

P

A

H

E

N

L

A

A

M

H

H

L

I

V

I

A

R

D

G

A

Y

D

D

V

V

L

L

D

D

C

C

C

T

D

D

N

N

F

V

D

P

T

T

R

R

Q

Y

A

L

V

L

N

S

R

D

A

A

C

C

V

V

Q

M

H

L

S

S

V

K

P

P

L

L

V

V

S

S

G

G

A

S

A

A

L

L

R

K

F

M

D

D

G

G

Q

Q

I

L

S

T

V

V

F

Y

D

N

H

Y

R

-

Q

-

P

A

G

N

A

G

P

P

C

C

Y

Y

A

R

C

C

L

I

F

F

P

P

-

V

P

P

S

P

Q

P

P

P

A

E

Q

A

E

V

V

T

A

N

C

C

A

G

T

D

M

G

G

G

V

V

F

L

A

G

P

A

L

V

V

T

G

G

M

T

V

I

G

G

T

A

V

M

Q

Q

A

A

A

L

E

E

A

A

L

I

K

K

L

V

L

I

M

V

G

G

V

M

G

G

T

D

T

V

L

L

A

A

G

G

R

R

L

L

M

I

V

F

D

D

A

G

L

G

G

S

M

G

E

V

W

F

T

R

T

N

M

I

R

R

L

I

-

R

A

S

R

K

V

R

A

P

D

N

C

C

E

H

V

M

C

C

G

S

Sites not aligning to the query:

62 modified: Phosphothreonine

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
40% identity, 96% coverage: 1:246/255 of query aligns to 61:318/482 of O59954

query
sites
O59954

M

L

N

H

D

G

D

E

Q

E

L

Y

L

R

R

R

Y

Y

S

G

R

R

H

Q

I

M

L

I

L

V

D

P

E

Q

L

F

G

G

I

L

E

Q

G
|
G

Q

Q

S

L

R

K

L

L

L

R

A

D

S

A

H

K

A

V

L

L

V

I

I

V

G

G

A

A

G

G

L

L

G
|
G

A

C

A

P

A

A

L

A

P

L

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

T

I

I

T

G

V

L

V

V

D

D

D

G

D

D

V

T

V

V

D

E

L

A

T

S

N

N

L

L

Q

H

R
|
R

Q

Q

V

V

I

L

H

H

T

R

T

S

A

R

N

N

V

V

G

G

R

K

P

L

K

K

V

V

E

D

S

S

A

A

R

I

E

E

G

Y

M

L

L

R

R

E

I

L

N

N

P

P

G

H

I

P

D

T

V

Y

R

I

L

A

V

H

Q

Q

A

A

R

H

V

L

G

T

A

P

A

R

E

E

L

A

A

P

H

D

L

I

V

F

A

K

D

D

A

Y

D

D

V

L

V

I

L

L

D

D

C
|
C

C

T

D

D

N

N

F

P

D

A

T

T

R

R

Q

Y

A

L

V

I

N

S

R

D

A

T

C

A

V

V

Q

L

H

L

S

G

V

K

P

P

L

L

V

V

S

S

G

A

A

S

A

A

L

L

R

R

F

T

D
x
E

G

G

Q

Q

I

L

S

M

V

V

F

L

D

N

H

N

-

P

R

P

Q

Q

P

P

-

P

-

G

-

D

-

K

-

T

G

G

A

G

P

P

C

C

Y

Y

A

R

C

C

L

V

F

F

P

P

P

K

S

P

Q

P

P

P

A

A

Q

N

E

S

V

V

-

T

A

S

C

C

A

A

T

D

M

G

G

G

V

I

F

L

A

G

P

P

L

V

V

V

G
|
G

M

T

V

M

G

G

T

V

V

L

Q

Q

A

A

A

S

E

E

A

A

L

I

K

K

L

V

L

L

M

T

G

S

V

A

G

G

T

E

T

S

L

V

A

E

G

A

-

T

-

P

R

S

L

L

M

I

V

F

D

S

A

A

L

Y

G

S

M

S

-

P

E

Q

W

F

T

R

T

T

M

I

R

K

L

L

-

R

A

S

R

R

V

R

A

P

D

N

C

C

E

A

V

V

C

C

G82 (= G22) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G40) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R70) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (= C125) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ D155) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G197) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
45% identity, 97% coverage: 1:247/255 of query aligns to 55:301/460 of O95396

query
sites
O95396

M

L

N

S

D

R

D

D

Q

E

L

I

L

L

R

R

Y

Y

S

S

R

R

H

Q

I

L

L

V

L

L

D

P

E

E

L

L

G

G

I

V

E

H

G

G

Q

Q

S

L

R

R

L

L

L

G

A

T

S

A

H

C

A

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

G

G

A

C

A

P

A

L

L

A

P

Q

Y

Y

L

L

A

A

S

A

A

A

G

G

V

V

G

G

T

R

I

L

T

G

V

L

V

V

D

D

D

Y

D

D

V

V

D

E

L

M

T

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

I

L

H

H

T

G

T

E

A

A

N

L

V

A

G

G

R

Q

P

A

K

K

V

A

E

F

S

S

A

A

R

A

E

A

G

S

M

L

L

R

R

R

I

L

N

N

P

S

G

A

I

V

D

E

-

C

V

V

R

P

L

Y

V
x
T

Q
|
Q

A
|
A

R
x
L

V
x
T

G
x
P

A
|
A

A
x
T

E
x
A

L
|
L

A

-

H
x
D

L
|
L

V
|
V

A
x
R

D
x
R

A
x
Y

D
|
D

V
|
V

L
x
A

D
|
D

C
|
C

C
x
S

D
|
D

N
|
N

F
x
V

D
x
P

T
|
T

R
|
R

Q
x
Y

A
x
L

V
|
V

N
|
N

R
x
D

A
|
A

C
|
C

V
|
V

Q
x
L

H
x
A

S
x
G

V
x
R

P
|
P

L
|
L

V
|
V

S
|
S

G
x
A

A
x
S

A
|
A

L
|
L

R
|
R

F
|
F

D
x
E

G
|
G

Q
|
Q

I
|
I

S
x
T

V
|
V

F
x
Y

D
x
H

H
x
Y

R
x
D

Q

-

P

-

G
|
G

A
x
G

P
|
P

C
|
C

Y
|
Y

A
x
R

C
|
C

L
x
I

F
|
F

P
|
P

P
x
Q

S
x
P

Q
x
P

P
|
P

A
|
A

Q
x
E

E
x
T

V
|
V

A
x
T

-
x
N

C
|
C

A

A

T

D

M

G

G

G

V

V

F

L

A

G

P

V

L

V

V

T

G

G

M

V

V

L

G

G

T

C

V

L

Q

Q

A

A

A

L

E

E

A

V

L

L

K

K

L

I

L

A

M

A

G

G

V

L

G

G

T

P

T

S

L

Y

A

S

G

G

R

S

L

L

M

L

V

F

D

D

A

A

L

L

-

R

G

G

M

H

E

F

W

R

T

S

T

I

M

R

R

L

L

R

A

S

R

R

V

R

A

L

D

D

C

C

E

A

V

A

C

C

G

G

158:238 (vs. 103:185, 50% identical) Interaction with NFS1
C239 (= C186) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
43% identity, 98% coverage: 2:252/255 of query aligns to 4:258/271 of Q72J02

query
sites
Q72J02

N

T

D

K

D

E

Q

E

L

L

L

D

R

R

Y

Y

S

H

R

R

H

Q

I

M

L

I

L

L

D

P

E

Q

L

V

G

G

I

P

E

E

G

G

Q

Q

S

E

R

R

L

L

L

K

A

R

S

A

H

S

A

V

L

V

V

V

I

V

G

G

A

A

G

G

L

L

G

G

A

V

A

P

A

V

L

L

P

Q

Y

Y

L

L

A

V

S

A

A

A

G

G

V

V

G

G

T

R

I

V

T

G

V

V

D

E

D

M

D

D

V

R

V

V

D

E

L

V

T

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

L

H

Y

T

T

A

E

N

D

V

V

G

G

R

E

P

P

K

K

V

A

E

L

S

V

A

A

R

Q

E

K

G

R

M

L

L

Q

R

A

I

L

N

N

P

P

G

L

I

V

D

R

V

V

R

E

L

A

V

Y

Q

P

A

V

R

R

V

L

G

T

A

S

A

E

E

N

L

A

A

L

H

E

L

I

V

L

A

R

D

P

A

Y

D

D

V

L

V

V

L

V

D

D

C

A

C

S

D

D

N

N

F

F

D

P

T

T

R

R

Q

Y

A

L

V

V

N

N

R

D

A

A

C

A

V

V

Q

L

H

L

S

G

V

K

P

P

L

L

V

V

S

F

G

G

A

A

A

I

L

Y

R

Q

F

F

D

D

G

G

Q

Q

I

V

S

A

V

V

F

F

D

H

H

H

-

P

-

T

-

L

R

H

Q

G

P

E

G

M

A

G

P

P

C

C

Y

Y

A

R

C

C

L

L

F

F

P

P

P

K

S

P

Q

P

P

P

A

P

Q

G

E

A

V

V

-

P

A

S

C
|
C

A

A

T

E

M

A

G

G

V

V

F

F

A

G

P

V

L

L

V

P

G

A

M

V

V

V

G

G

T

S

V

L

Q

M

A

A

E

E

A

A

L

L

K

K

L

V

L

L

M

L

G

G

V

I

G

G

T

K

T

P

L

L

A

A

G

G

R

H

L

L

M

L

V

Y

D

D

A

A

L

L

G

E

M

A

E

S

W

F

T

R

T

K

M

L

R

T

L

V

A

R

R

R

V

N

A

P

D

R

C

C

E

P

V

V

C

C

G

G

G

D

A

E

H

P

A

T

Q

Q

C192 (= C186) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
37% identity, 96% coverage: 4:247/255 of query aligns to 42:290/440 of P38820

query
sites
P38820

D

E

Q

E

L

Y

L

Q

R

R

Y

Y

S

G

R

R

H

Q

I

M

L

I

L

V

D

E

E

E

L

T

G

G

-

G

I

V

E

A

G

G

Q

Q

S

V

R

K

L

L

L

K

A

N

S

T

H

K

A

V

L

L

V

V

I

V

G

G

A

A

G

G

L

L

G

G

A

C

A

P

A

A

L

L

P

P

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

Q

I

I

T

G

V

I

V

V

D

D

D

N

D

D

V

V

D

E

L

T

T

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

L

H

H

T

D

T

S

A

S

N

R

V

V

G

G

R

M

P

L

K

K

V

C

E

E

S

S

A

A

R

R

E

Q

G

Y

M

I

L

T

R

K

I

L

N

N

P

P

G

H

I

I

D

N

V

V

R

V

L

T

V

Y

Q

P

A

V

R

R

V

L

G

N

A

S

E

N

L

A

A

F

H

D

L

I

V

F

A

K

D

G

A

Y

D

N

V

Y

V

I

L

L

D

D

C

C

C

T

D

D

N

S

F

P

D

L

T

T

R

R

Q

Y

A

L

V

V

N

S

R

D

A

V

C

A

V

V

Q

N

H

L

S

G

V

I

P

T

L

V

V

V

S

S

G

A

A

S

A

G

L

L

R

G

F

T

D

E

G

G

Q

Q

I

L

S

T

V

I

F

L

D

N

H

F

R

N

Q

N

P

I

G

G

A

-

P

P

C

C

Y

Y

A

R

C

C

L

F

F

Y

P

P

-

T

P

P

S

P

Q

P

P

P

A

N

Q

A

E

V

V

T

A

S

C
|
C

A

Q

T

E

M

G

G

G

V

V

F

I

A

G

P

P

L

C

V

I

G

G

M

L

V

V

G

G

T

T

V

M

Q

M

A

A

A

V

E

E

A

T

L

L

K

K

L

L

L

I

M

L

G

G

V

I

G

Y

T

T

T

N

L

E

A

N

G

F

R

S

L

P

L

F

M

L

V

M

D

L

A

Y

L

S

G

G

M

F

E

P

W

Q

T

Q

T

S

M

L

R

R

L

T

-

F

-

K

-

M

-

R

A

G

R

R

V

Q

A

E

D

K

C

C

E

L

V

C

C

C

G

G

C225 (= C186) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 83% coverage: 1:212/255 of query aligns to 7:213/289 of 6yubB

query
sites
6yubB

M

L

N

S

D

K

D

E

Q

E

L

I

L

E

R

R

Y

Y

S

S

R

R

H

Q

I

M

L

I

L

V

D

P

E

G

L

M

G

G

I

K

E

E

G

G

Q

Q

S

L

R

R

L

L

L

M

A

N

S

A

H

K

A

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

A

P

A

A

L

A

P

Q

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

T

I

I

T

G

V

I

V

V

D

D

D

G

D

D

V

S

V

V

D

E

L

T

T

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

T

A

T

T

A

K

N

R

V

V

G

G

R

M

P

L

K

K

V

V

E

D

S

S

A

L

R

I

E

T

G

H

M

L

L

I

R

E

I

I

N

N

P

P

G

L

I

P

D

V

V

Y

R

V

L

P

V

Y

Q

R

A

F

R

D

V

L

G

T

A

P

A

Q

E

N

L

A

A

A

H

Q

L

I

V

I

A

K

D

P

A

W

D

D

V

V

V

I

L

L

D

D

C

C

C

T

D

D

N

N

F

P

D

A

T

T

R

R

Q

Y

A

L

V

I

N

S

R

D

A

V

C

C

V

V

Q

L

H

L

S

G

V

K

P

P

L

L

V

V

S

S

G

A

A

A

A

S

L

V

R

Q

F

K

D

S

G

G

Q

Q

I

L

S

I

V

V

F

L

D

N

H

C

-

P

-

T

-

P

-

Q

-

G

-

V

R

N

Q

K

P

K

G

A

A

A

P

P

C
|
C

Y

Y

A

R

C
|
C

L

C

F

F

P

-

S

-

Q

-

P

-

A

-

Q

-

E

-

V

-

A

-

C

-

A

-

T

-

M

K

G

G

V

I

F

M

A

G

P

P

L

V

V

V

G

G

M

M

V

M

G

G

T

V

V

A

Q

Q

A

A

A

G

E

E

A

A

L

I

K

K

L

I

L

L

M

V

binding zinc ion: C184 (= C170), C187 (= C173)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 83% coverage: 1:212/255 of query aligns to 6:214/423 of 6yubA

query
sites
6yubA

M

L

N

S

D

K

D

E

Q

E

L

I

L

E

R

R

Y

Y

S

S

R

R

H

Q

I

M

L

I

L

V

D

P

E

G

L

M

G

G

I

K

E

E

G

G

Q

Q

S

L

R

R

L

L

L

M

A

N

S

A

H

K

A

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

A

P

A

A

L

A

P

Q

Y

Y

L

L

A

A

S

G

A

A

G

G

V

V

G

G

T

T

I

I

T

G

V

I

V

V

D

D

D

G

D

D

V

S

V

V

D

E

L

T

T

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

T

A

T

T

A

K

N

R

V

V

G

G

R

M

P

L

K

K

V

V

E

D

S

S

A

L

R

I

E

T

G

H

M

L

L

I

R

E

I

I

N

N

P

P

G

L

I

P

D

V

V

Y

R

V

L

P

V

Y

Q

R

A

F

R

D

V

L

G

T

A

P

A

Q

E

N

L

A

A

A

H

Q

L

I

V

I

A

K

D

P

A

W

D

D

V

V

V

I

L

L

D

D

C

C

C

T

D

D

N

N

F

P

D

A

T

T

R

R

Q

Y

A

L

V

I

N

S

R

D

A

V

C

C

V

V

Q

L

H

L

S

G

V

K

P

P

L

L

V

V

S

S

G

A

A

A

A

S

L

V

R

Q

F

K

D

S

G

G

Q

Q

I

L

S

I

V

V

F

L

D

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

H

V

R

N

Q

K

P

K

G

A

A

A

P

P

C
|
C

Y

Y

A

R

C
|
C

L

C

F

F

P

-

S

K

Q

K

P

P

A

-

Q

-

E

-

V

-

A

-

C

-

A

-

T

-

M

-

G

G

V

I

F

M

A

G

P

P

L

V

V

V

G

G

M

M

V

M

G

G

T

V

V

A

Q

Q

A

A

A

G

E

E

A

A

L

I

K

K

L

I

L

L

M

V

binding zinc ion: C183 (= C170), C186 (= C173)

Sites not aligning to the query:

binding zinc ion: 266, 269

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 73% coverage: 20:206/255 of query aligns to 17:223/628 of O42939

query
sites
O42939

I

V

E

E

G

A

Q

L

S

R

R

N

L

F

L

K

A

S

S

A

H

K

A

V

L

L

V

L

I

V

G

G

A

A

G

G

L

I

G

G

A

C

A

E

A

L

L

L

P

K

Y

N

L

L

A

L

S

M

A

S

G

G

V

V

G

K

T

E

I

V

T

H

V

I

D

D

D

L

D

D

V

T

V

I

D

D

L

L

T

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

T

R

T

K

A

K

N

H

V

V

G

K

R

Q

P

P

K

K

V

A

E

I

S

V

A

A

R

A

E

K

G

T

M

A

L

S

R

S

I

F

N

N

P

P

G

N

I

V

D

K

V

L

R

E

L

A

V

Y

Q

H

A

A

R

N

V

I

G

K

A

E

A

D

E

R

L

F

-

N

A

V

H

A

L

W

V

F

A

R

D

Q

A

F

D

D

V

L

V

V

L

F

D

N

C

A

C

L

D

D

N

N

F

L

D

D

T

A

R

R

Q

R

A

H

V

V

N

N

R

K

A

Q

C

C

V

L

Q

L

H

A

S

S

V

V

P

P

L

L

V

I

S

E

G

S

A

G

A

T

L

T

R

G

F

F

D

L

G

G

Q

Q

I

V

S

Q

V

V

F

I

D

I

H

H

R

G

Q

K

P

-

G

-

A

T

P

E

C

C

Y

Y

A

D

C

C

-

N

-

P

-

K

-

E

-

P

-

K

L

T

F

Y

P

P

-

V

-

C

-

T

-

I

-

R

-

S

-

T

P

P

S

S

Q

Q

P

P

A

I

Q

H

E

C

V

V

A

V

C

W

A

A

T

K

M

S

G

Y

V

F

F

F

A

P

P

Q

L

L

V

F

-

S

-

N

-

D

-

Q

-

E

-

S

-

D

G

G

M

I

V

I

G

D

T

N

V

V

Q

S

A

A

A

N

E

E

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
30% identity, 60% coverage: 26:179/255 of query aligns to 16:168/650 of Q7SXG4

query
sites
Q7SXG4

L

L

L

S

A

S

S

C

H

R

A

V

L

L

V

V

I

V

G

G

A

A

G

G

L

I

G

G

A

C

A

E

A

L

L

L

P

K

Y

N

L

L

A

V

S

L

A

T

G

G

V

F

G

K

T

N

I

I

T

E

V

V

V

I

D

D

D

L

D

D

V

T

V

I

D

D

L

V

T

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

T

Q

T

K

A

K

N

H

V

V

G

G

R

K

P

S

K

K

V

A

E

Q

S

V

A

A

R

K

E

E

G

S

M

V

L

L

R

R

I

F

N

C

P

P

G

S

I

A

D

N

V

I

R

T

L

A

V

Y

Q

H

A

D

R

S

V

I

G

M

A

N

A

P

E

D

L

Y

-

N

A

V

H

E

L

F

V

F

A

R

D

N

A

F

D

Q

V

L

V

V

L

M

D

N

C

A

C

L

D

D

N

N

F

R

D

A

T

A

R

R

Q

N

A

H

V

V

N

N

R

R

A

M

C

C

V

L

Q

A

H

A

S

D

V

I

P

P

L

L

V

I

S

E

G

S

A

G

A

T

L

A

R

G

F

Y

D

L

G

G

Q

Q

I

V

S

T

V

V

F

I

D

K

H

K

R

G

Q

Q

P

-

G

-

A

T

P

E

C

C

Y

Y

A

E

C

C

L

Q

F

P

P

K

P

P

S

T

Q

Q

Sites not aligning to the query:

599 modified: Phosphoserine

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
25% identity, 97% coverage: 2:248/255 of query aligns to 85:340/340 of 3h5rA

query
sites
3h5rA

N

N

D

S

D

T

Q

E

L

N

R

R

Y

Y

S

S

R

R

H

N

I

F

L

L

-

H

L

Y

D

Q

E

S

L

Y

G

G

I

A

E

N

G

P

-

V

-

L

-

V

Q

Q

S

D

R

K

L

L

L

K

A

N

S

A

H

K

A

V

L

V

V

I

I

L

G

G

A

C

G

G

L
x
I

G

G

A

N

A

H

A

V

L

S

P

V

Y

I

L

L

A

A

S

T

A

S

G

G

V

I

G

G

T

E

I

I

T

I

V

L

V

I

D

D

D

N

D

D

V

Q

V

I

D

E

L

N

T

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

T

S

T

E

A

D

N

D

V

V

G

G

R

K

P

N

K

K

V

T

E

E

S

V

A

I

R

K

E

R

G

E

M

L

L

L

R

K

I

R

N

N

P

S

G

E

I

I

D

S

V

V

R

S

L

E

V

I

Q

A

A

L

R

N

V

I

G

N

A

D

A

Y

E

T

L

D

A

L

H

H

L

K

V

V

A

P

D

E

A

A

D

D

V

I

V

W

L

V

D

V

C
x
S

C
x
A

D

D

N

H

-

P

F

F

D

N

T

L

R

I

Q

N

A

W

V

V

N

N

R

K

A

Y

C

C

V

V

Q

R

H

A

S

N

V

Q

P

P

L

Y

V

I

S

N

G
x
A

A
x
G

A
x
Y

L

V

R

-

F

-

D

-

G

N

Q

D

I

I

S

A

V
|
V

F

F

D

G

H

P

R

L

Q

Y

-

V

P

P

G

G

A

K

P

T

-

G

C
|
C

Y

Y

A

E

C
|
C

L

Q

F

K

P

V

P

K

S

E

Q

N

P

I

A

D

Q

H

E

K

V

I

A

K

C

L

A

I

T

N

M

S

-

R

-

F

-

K

-
x
P

G

A

V

T

F

F

A

A

P

P

L

V

V

N

G

N

M

V

V

A

G

A

T

A

V

L

Q

C

A

A

E

D

A

V

L

I

K

K

L

F

L

I

M

G

G

K

V

Y

G

S

T

E

T

P

L

L

A

S

-

L

G

N

R

K

L
x
R

L

I

M
x
G

V

I

D

W

A

S

L

D

G

E

M

I

E

K

W

I

T

H

T

S

M

Q

R

N

L

M

A

G

R

R

V

S

A

P

D

V

C
|
C

E

S

V

V

C
|
C

G

G

G

N

active site: R157 (= R70)
binding zinc ion: C257 (= C170), C260 (= C173), C335 (= C243), C338 (= C246)
binding : I126 (≠ L39), S212 (≠ C125), A213 (≠ C126), A237 (≠ G149), G238 (≠ A150), Y239 (≠ A151), V245 (= V160), P280 (vs. gap), R314 (≠ L222), G316 (≠ M224)

Sites not aligning to the query:

binding : 10, 14, 26

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
25% identity, 97% coverage: 2:248/255 of query aligns to 85:339/339 of 3h9jA

query
sites
3h9jA

N

N

D

S

D

T

Q

E

L

N

R

R

Y

Y

S

S

R

R

H

N

I

F

L

L

-

H

L

Y

D

Q

E

S

L

Y

G

G

I

A

E

N

G

P

-

V

-

L

-

V

Q

Q

S

D

R

K

L

L

L

K

A

N

S

A

H

K

A

V

L

V

V

I

I

L

G

G

A

C

G
|
G

L

I

G

G

A

N

A

H

A

V

L

S

P

V

Y

I

L

L

A

A

S

T

A

S

G

G

V

I

G

G

T

E

I

I

T

I

V

L

V

I

D
|
D

D

N

D
|
D

V

Q

V

I

D

E

L

N

T

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

T

S

T

E

A

D

N

D

V

V

G

G

R

K

P

N

K
|
K

V

T

E

E

S

V

A

I

R

K

E

R

G

E

M

L

L

L

R

K

I

R

N

N

P

S

G

E

I

I

D

S

V

V

R

S

L

E

V

I

Q

A

A

L

R
x
N

V
x
I

G

N

A

D

A

Y

E

T

L

D

A

L

H

H

L

K

V

V

A

P

D

E

A

A

D

D

V

I

V

W

L

V

D

V

C

S

C
x
A

D
|
D

N
x
H

-

P

F

F

D

N

T

L

R

I

Q

N

A

W

V

V

N

N

R

K

A

Y

C

C

V

V

Q

R

H

A

S

N

V

Q

P

P

L

Y

V

I

S

N

G

A