SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing RR42_RS03715 FitnessBrowser__Cup4G11:RR42_RS03715 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5eq9B Crystal structure of medicago truncatula histidinol-phosphate phosphatase (mthpp) in complex with l-histidinol phosphate and mg2+ (see paper)
46% identity, 95% coverage: 6:254/263 of query aligns to 1:253/260 of 5eq9B

query
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5eq9B

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active site: D39 (= D51), E62 (= E74), D80 (= D90), I82 (= I92), D83 (= D93), T85 (= T95), D204 (= D214)
binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: D83 (= D93), G84 (= G94), T85 (= T95), L156 (≠ M165), D180 (= D190), S198 (≠ A208), L200 (= L210)
binding magnesium ion: D80 (= D90), D83 (= D93), Y203 (= Y213), D204 (= D214)

5eq8A Crystal structure of medicago truncatula histidinol-phosphate phosphatase (mthpp) in complex with l-histidinol (see paper)
46% identity, 94% coverage: 7:254/263 of query aligns to 1:252/259 of 5eq8A

query
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active site: D38 (= D51), E61 (= E74), D79 (= D90), I81 (= I92), D82 (= D93), T84 (= T95), D203 (= D214)
binding L-histidinol: D82 (= D93), L155 (≠ M165), D179 (= D190), E196 (= E207), S197 (≠ A208), G198 (= G209), L199 (= L210)

5t3jA Histidinol phosphate phosphatase(hpp) soaked with selenourea for 10 min (see paper)
45% identity, 95% coverage: 6:254/263 of query aligns to 3:250/257 of 5t3jA

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5t3jA

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active site: D36 (= D51), E59 (= E74), D77 (= D90), I79 (= I92), D80 (= D93), T82 (= T95), D201 (= D214)
binding selenourea: Q4 (= Q7), F51 (≠ Y66), Y72 (≠ L85)

Q6NPM8 Bifunctional phosphatase IMPL2, chloroplastic; Histidinol-phosphatase; Histidinol-phosphate phosphatase; HPP; Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Protein HISTIDINE BIOSYNTHESIS 7; Protein MYO-INOSITOL MONOPHOSPHATASE-LIKE 2; EC 3.1.3.15; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 98% coverage: 3:259/263 of query aligns to 78:343/346 of Q6NPM8

query
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G195 (= G120) mutation to R: Loss of histidinol-phosphatase activity.

Q9K4B1 Histidinol-phosphatase; HolPase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
33% identity, 82% coverage: 46:260/263 of query aligns to 41:263/266 of Q9K4B1

query
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Q9K4B1

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A

A

A

E

G

G

H

S

V

V

D

D

A

L

V

C

V

A

E

E

A

P

G

E

L

L

K

S

A

L

Y

W

D

D

V

M

Q

A

A

A

L

N

I

A

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

T

M

F

T

T

S

G

W

L

T

D

G

G

G

R

D

P

A

G

Q

P

Q

H

G

S

G

G

T

N

V

A

A

A

C

S

G

-

D

N

P

G

R

R

L

L

H

H

Q

D

Q

E

I

L

A

G

L

Y

L

L

N

N

G118 (= G120) mutation to R: Requires histidine to grow.

P0ADG4 Nus factor SuhB; Inositol-1-monophosphatase; I-1-Pase; IMPase; Inositol-1-phosphatase; EC 3.1.3.25 from Escherichia coli (strain K12) (see 5 papers)
33% identity, 94% coverage: 12:257/263 of query aligns to 5:255/267 of P0ADG4

query
sites
P0ADG4

M

L

D

N

F

I

A

A

A

V

G

R

L

A

A

A

Q

R

A

K

A

A

G

G

N

N

A

L

S

I

L

A

P

K

Y

N

F

Y

R

E

S

T

A

P

P

D

A

A

V

V

E

E

D

A

K

S

G

Q

G

K

R

G

H

S

F

N

D

D

P

F

V

V

T

T

A

N

A

V

D

D

K

K

A

A

E

E

R

A

A

V

M

I

R

I

D

D

L

T

I

I

L

R

A

K

K

S

Y

Y

P

P

E

Q

H

H

G

T

I

I

L

I

G

T

E
|
E

E

E

E

S

D

G

R

E

V

L

A

E

G

G

T

T

S

D

P

Q

-

D

L

V

T

Q

W

W

V

V

L

I

D
|
D

P

P

I
x
L

D
|
D

G

G

T

T

R

T

A

N

F

F

I

I

T

K

G

R

L

L

P

P

L

H

W

F

G

A

T

V

L

S

I

I

A

A

L

V

N

R

D

I

G

K

Q

G

R

R

P

T

V

E

I

V

G

A

I

V

M

V

D

Y

Q

D

P

P

F

M

T

R

R

N

E

E

R

L

F

F

S

T

G

A

D

T

-

R

G

G

S

Q

R

G

A

A

W

Q

L

L

N

N

G

G

Q

Y

P
x
R

L

L

R

R

T

G

R

S

P

T

C

A

A

R

D

D

L

L

A

D

Q

G

A

T

K

I

L

L

M

A

C

T

T

G

T

F

P

P

E

-

M

-

F

F

E

K

D

-

A

A

A

K

Q

Q

F

Y

A

A

A

T

F

T

-

Y

-

I

-

N

-

I

-

V

-
x
G

R

K

R

L

V

F

A

N

D

E

A

C

A

A

R

D

M

F

Q
x
R

R
|
R

Y

T

G

G

G

S

D

A

C

A

Y

L

A

D

Y

L

C

A

M

Y

V

V

A

A

G

G

H

R

V

V

D

D

A

G

V

F

E

E

A

I

G

G

L

L

K

R

A

P

Y

W

D

D

V

F

Q

A

A

A

L

G

I

E

P

L

I

L

V

V

Q

R

G

E

A

A

G

G

V

I

M

V

T

S

S

D

W

F

T

T

G

G

D

H

A

N

Q

Y

Q

M

G

L

G

T

T

G

V

N

V

I

A

V

C

A

G

G

D

N

P

P

R

R

L

V

H
x
V

Q
x
K

Q
x
A

I
x
M

I
x
L

A

A

E67 (= E74) binding
D84 (= D90) binding
L86 (≠ I92) binding
D87 (= D93) mutation to N: Loss of IMPase activity, still complements dnaB121 helicase mutation.
R139 (≠ P144) mutation to C: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with A-250.
G173 (vs. gap) mutation to V: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNA polymerase (RNAP).
R183 (≠ Q185) mutation to A: Some growth at 30 degrees Celsius, greater IMPase activity, partially inhibited by RNAP.
R184 (= R186) mutation to A: Grows at 30 degrees Celsius, makes more dimer, partially inhibited by RNAP, crystallizes.; mutation to I: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNAP.
V250 (≠ H252) mutation to A: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with C-139.
KAML 251:254 (≠ QQII 253:256) mutation to AAMA: 3-fold decreased affinity for NusA, less efficient in delaying or suppressing Rho-dependent transcription termination.

6ib8B Structure of a complex of suhb and nusa ar2 domain (see paper)
33% identity, 94% coverage: 12:257/263 of query aligns to 9:259/270 of 6ib8B

query
sites
6ib8B

M

L

D

N

F

I

A

A

A

V

G

R

L

A

A

A

Q

R

A

K

A

A

G

G

N

N

A

L

S

I

L

A

P

K

Y

N

F

Y

R

E

S

T

A

P

P

D

A

A

V

V

E

E

D

A

K

S

G

Q

G

K

R

G

H

S

F

N

D

D

P

F

V

V

T

T

A

N

A

V

D

D

K

K

A

A

E

E

R

A

A

V

M

I

R

I

D

D

L

T

I

I

L

R

A

K

K

S

Y

Y

P

P

E

Q

H

H

G

T

I

I

L

I

G

T

E

E

E

S

D

G

R

E

V

L

A

E

G

G

T

T

S

D

P

Q

-

D

L

V

T

Q

W

W

V

V

L

I

D
|
D

P

P

I
x
L

D

D

G

G

T

T

R

T

A

N

F

F

I

I

T

K

G

R

L

L

P

P

L

H

W

F

G

A

T

V

L

S

I

I

A

A

L

V

N

R

D

I

G

K

Q

G

R

R

P

T

V

E

I

V

G

A

I

V

M

V

D

Y

Q

D

P

P

F

M

T

R

R

N

E

E

R

L

F

F

S

T

G

A

D

T

-

R

G

G

S

Q

R

G

A

A

W

Q

L

L

N

N

G

G

Q

Y

P

R

L

L

R

R

T

G

R

S

P

T

C

A

A

R

D

D

L

L

A

D

Q

G

A

T

K

I

L

L

M

A

C

T

T

G

T

F

P

P

E

-

M

-

F

F

E

K

D

-

A

A

A

K

Q

Q

F

Y

A

A

A

T

F

T

-

Y

-

I

-

N

-

I

-

V

-

G

R

K

R

L

V

F

A

N

D

E

A

C

A

A

R

D

M

F

Q

R

R

R

Y

T

G

G

G

S

D

A

C

A

Y

L

A

D

Y

L

C

A

M

Y

V

V

A

A

G

G

H

R

V

V

D

D

A

G

V

F

E

E

A

I

G

G

L

L

K

R

A

P

Y

W

D

D

V

F

Q

A

A

A

L

G

I

E

P

L

I

L

V

V

Q

R

G

E

A

A

G

G

V

I

M

V

T

S

S

D

W

F

T

T

G

G

D

H

A

N

Q

Y

Q

M

G

L

G

T

T

G

V

N

V

I

A

V

C

A

G

G

D

N

P

P

R

R

L

V

H

V

Q

K

Q

A

I

M

I

L

A

A

binding magnesium ion: D88 (= D90), L90 (≠ I92)

2qflA Structure of suhb: inositol monophosphatase and extragenic suppressor from e. Coli (see paper)
32% identity, 94% coverage: 12:257/263 of query aligns to 5:255/262 of 2qflA

query
sites
2qflA

M

L

D

N

F

I

A

A

A

V

G

R

L

A

A

A

Q

R

A

K

A

A

G

G

N

N

A

L

S

I

L

A

P

K

Y

N

F

Y

R

E

S

T

A

P

P

D

A

A

V

V

E

E

D

A

K

S

G

Q

G

K

R

G

H

S

F

N

D

D

P

F

V

V

T

T

A

N

A

V

D
|
D

K

K

A

A

E

E

R

A

A

V

M

I

R

I

D

D

L

T

I

I

L

R

A

K

K

S

Y

Y

P

P

E

Q

H

H

G

T

I

I

L

I

G

T

E
|
E

E

E

E

S

D

G

R

E

V

L

A

E

G

G

T

T

S

D

P

Q

-

D

L

V

T

Q

W

W

V

V

L

I

D
|
D

P

P

I
x
L

D
|
D

G

G

T
|
T

R

T

A

N

F

F

I

I

T

K

G

R

L

L

P

P

L

H

W

F

G

A

T

V

L

S

I

I

A

A

L

V

N

R

D

I

G

K

Q

G

R

R

P

T

V

E

I

V

G

A

I

V

M

V

D

Y

Q

D

P

P

F

M

T

R

R

N

E

E

R

L

F

F

S

T

G

A

D

T

-

R

G

G

S

Q

R

G

A

A

W

Q

L

L

N

N

G

G

Q

Y

P

R

L

L

R

R

T

G

R

S

P

T

C

A

A

R

D

D

L

L

A

D

Q

G

A

T

K

I

L

L

M

A

C

T

T

G

T

F

P

P

E

-

M

-

F

F

E

K

D

-

A

A

A

K

Q

Q

F

Y

A

A

A

T

F

T

-

Y

-

I

-

N

-

I

-

V

-

G

R

K

R

L

V

F

A

N

D

E

A

C

A

A

R

D

M

F

Q

R

R

A

Y

T

G
|
G

G
x
S

D
x
A

C

A

Y

L

A

D

Y

L

C

A

M

Y

V

V

A

A

G

G

H

R

V

V

D

D

A

G

V

F

E

E

A

I

G

G

L

L

K

R

A

P

Y

W

D
|
D

V

F

Q

A

A

A

L

G

I

E

P

L

I

L

V

V

Q

R

G

E

A

A

G

G

V

I

M

V

T

S

S

D

W

F

T

T

G

G

D

H

A

N

Q

Y

Q

M

G

L

G

T

T

G

V

N

V

I

A

V

C

A

G

G

D

N

P

P

R

R

L

V

H

V

Q

K

Q

A

I

M

I

L

A

A

active site: D44 (= D51), E67 (= E74), D84 (= D90), L86 (≠ I92), D87 (= D93), T89 (= T95), D212 (= D214)
binding ethyl acetate: D87 (= D93), G186 (= G188), S187 (≠ G189), A188 (≠ D190)

P95189 Histidinol-phosphatase; HolPase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 82% coverage: 46:261/263 of query aligns to 39:259/260 of P95189

query
sites
P95189

P

P

V

V

T

T

A

D

A

A

D

D

K

R

A

A

A

V

E

E

R

S

A

D

M

V

R

R

D

Q

L

T

I

L

L

G

A

R

K

D

Y

R

P

P

E

G

H

D

G

G

I

V

L

L

G

G

E

E

E

-

D

-

R

-

V

F

A

G

G

G

T

S

S

T

P

T

L

F

T

T

-

G

-

R

-

Q

W

W

V

I

L

V

D

D

P

P

I

I

D
|
D

G

G

T

T

R

K

A

N

F

F

I

V

T

R

G

G

L

V

P

P

L

V

W

W

G

A

T

S

L

L

I

I

A

A

L

L

N

L

D

E

G

D

Q

G

R

V

P

P

V

S

I

V

G

G

I

V

M

V

D

S

Q

A

P

P

F

A

T

L

R

Q

E

R

R

R

F

W

-

W

-

A

S

R

G

G

D

R

G

G

S

A

R

F

A

A

W

S

L

V

N

D

G

G

-

A

-

R

-

P

Q

H

P

R

L

L

R

S

T

V

R

S

P

S

C

V

A

A

D

E

L

L

A

H

Q

S

A

A

K

S

L

L

M

S

C

F

T

S

P

L

E

S

M

G

F

W

E

A

D

R

A

P

A

G

Q

L

F

R

A

E

A

R

F

F

R

I

R

G

V

L

A

T

D

D

A

T

A

V

R

W

M

R

Q

V

R

R

Y

A

G

Y

G

G

D

D

C

F

Y

L

A

S

Y

Y

C

C

M

L

V

V

A

A

A

E

G

G

H

A

V

V

D

D

A

I

V

A

E

E

A

P

G

Q

L

V

K

S

A

V

Y

W

D

D

V

L

Q

A

A

A

L

L

I

D

P

I

I

V

V

V

Q

R

G

E

A

A

G

G

V

R

M

L

T

T

S

S

W

L

T

D

G

G

G

V

D

A

A

G

Q

P

Q

H

G

G

T

S

V

A

V

V

A

A

C

T

G

-

D

N

P

G

R

L

L

L

H

H

Q

D

Q

E

I

V

I

L

A

T

L

R

L

L

N

N

A

A

D86 (= D93) mutation to N: Causes a probable loss of enzymatic activity.

6tqoT Rrn anti-termination complex (see paper)
34% identity, 81% coverage: 45:257/263 of query aligns to 30:247/255 of 6tqoT

query
sites
6tqoT

D

D

P

F

V

V

T

T

A

N

A

V

D

D

K

K

A

A

E

E

R

A

A

V

M

I

R

I

D

D

L

T

I

I

L

R

A

K

K

S

Y

Y

P

P

E

Q

H

H

G

T

I

I

L

I

G

T

E
|
E

E

E

E

S

D

G

R

E

V

L

A

E

G

G

T

T

S

D

P

Q

-

D

L

V

T

Q

W

W

V

V

L

I

D
|
D

P

P

I
x
L

D

D

G

G

T

T

R

T

A

N

F

F

I

I

T

K

G

R

L

L

P

P

L

H

W

F

G

A

T

V

L

S

I

I

A

A

L

V

N

R

D

I

G

K

Q

G

R

R

P

T

V

E

I

V

G

A

I

V

M

V

D

Y

Q

D

P

P

F

M

T

R

R

N

E

E

R

L

F

F

S

T

G

A

D

T

-

R

G

G

S

Q

R

G

A

A

W
x
Q

L

L

N

N

G

G

Q

Y

P

R

L

L

R
|
R

T

G

R

S

P

T

C

A

A

R

D

D

L

L

A

D

Q

G

A

T

K

I

L

L

M

A

C

T

T

G

T

F

P

P

E

-

M

-

F

F

E

K

D

-

A

A

A

K

Q

Q

F

Y

A

A

A

T

F

T

-

Y

-

I

-

N

-

I

-

V

-

G

R

K

R

L

V

F

A

N

D

E

A

C

A

A

R

D

M

F

Q

R

R

R

Y

T

G

G

G

S

D

A

C

A

Y

L

A

D

Y

L

C

A

M

Y

V

V

A

A

G

G

H

R

V

V

D

D

A

G

V

F

E

E

A

I

G

G

L

L

K

R

A

P

Y

W

D

D

V

F

Q

A

A

A

L

G

I

E

P

L

I

L

V

V

Q

R

G

E

A

A

G

G

V

I

M

V

T

S

S

D

W

F

T

T

G

G

D

H

A

N

Q

Y

Q

M

G

L

G

T

T

G

V

N

V

I

A

V

C

A

G

G

D

N

P

P

R

R

L

V

H

V

Q

K

Q

A

I

M

I

L

A

A

binding magnesium ion: E59 (= E74), D76 (= D90), L78 (≠ I92)
binding : Q126 (≠ W139), R133 (= R146)

5yhtA Crystal structure of a phosphatase from mycobacterium tuberculosis in complex with its substrate (see paper)
33% identity, 82% coverage: 46:260/263 of query aligns to 36:255/255 of 5yhtA

query
sites
5yhtA

P

P

V

V

T

T

A

D

A

A

D
|
D

K

R

A

A

A

V

E

E

R

S

A

D

M

V

R

R

D

Q

L

T

I

L

L

G

A

R

K

D

Y

R

P

P

E

G

H

D

G

G

I

V

L

L

G

G

E
|
E

E

E

E

-

D

-

R

-

V

F

A

G

G

G

T

S

S

T

P

T

L

F

T

T

-

G

-

R

-

Q

W

W

V

I

L

V

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

R

K

A

N

F

F

I

V

T

R

G

G

L

V

P

P

L

V

W

W

G

A

T

S

L

L

I

I

A

A

L

L

N

L

D

E

G

D

Q

G

R

V

P

P

V

S

I

V

G

G

I

V

M

V

D

S

Q

A

P

P

F

A

T

L

R

Q

E

R

R

R

F

W

-

W

-

A

S

R

G

G

D

R

G

G

S

A

R

F

A

A

W

S

L

V

N

D

G

G

-

A

-

R

-

P

Q

H

P

R

L

L

R

S

T

V

R

S

P

S

C

V

A

A

D

E

L

L

A

H

Q

S

A

A

K

S

L

L

M

S

C

F

T

S

P

L

E

S

M

G

F

W

E

A

D

R

A

P

A

G

Q

L

F

R

A

E

A

R

F

F

R

I

R

G

V

L

A

T

D

D

A

T

A

V

R

W

M
x
R

Q

V

R
|
R

Y

A

G

Y

G

G

D
|
D

C

F

Y

L

A

S

Y

Y

C

C

M

L

V

V

A

A

A

E

G

G

H

A

V

V

D

D

A

I

V

A

E

E

A

P

G

Q

L

V

K

S

A

V

Y

W

D
|
D

V

L

Q

A

A

A

L

L

I

D

P

I

I

V

V

V

Q

R

G

E

A

A

G

G

V

R

M

L

T

T

S

S

W

L

T

D

G

G

G

V

D

A

A

G

Q

P

Q

H

G

G

T

S

V

A

V

V

A

A

C

T

G

-

D

N

P

G

R

L

L

L

H

H

Q

D

Q

E

I

V

I

L

A

T

L

R

L

L

N

N

binding phosphoric acid mono-[2-amino-3-(3h-imidazol-4-yl)-propyl]ester: E64 (= E74), I82 (= I92), D83 (= D93), G84 (= G94), T85 (= T95), D186 (= D190), D210 (= D214)
binding phosphate ion: R180 (≠ M184), R182 (= R186)
binding zinc ion: D41 (= D51), E64 (= E74), D80 (= D90), D80 (= D90), D210 (= D214)

5zonA Histidinol phosphate phosphatase from mycobacterium tuberculosis (see paper)
33% identity, 82% coverage: 46:260/263 of query aligns to 37:256/256 of 5zonA

query
sites
5zonA

P

P

V

V

T

T

A

D

A

A

D
|
D

K

R

A

A

A

V

E

E

R

S

A

D

M

V

R

R

D

Q

L

T

I

L

L

G

A

R

K

D

Y

R

P

P

E

G

H

D

G

G

I

V

L

L

G

G

E
|
E

E

-

D

-

R

-

V

F

A

G

G

G

T

S

S

T

P

T

L

F

T

T

-

G

-

R

-

Q

W

W

V

I

L

V

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

R

K

A

N

F

F

I

V

T

R

G

G

L

V

P

P

L

V

W

W

G

A

T

S

L

L

I

I

A

A

L

L

N

L

D

E

G

D

Q

G

R

V

P

P

V

S

I

V

G

G

I

V

M

V

D

S

Q

A

P

P

F

A

T

L

R

Q

E

R

R

R

F

W

-

W

-

A

S

R

G

G

D

R

G

G

S

A

R

F

A

A

W

S

L

V

N

D

G

G

-

A

-

R

-

P

Q

H

P

R

L

L

R

S

T

V

R

S

P

S

C

V

A

A

D

E

L

L

A

H

Q

S

A

A

K

S

L

L

M

S

C

F

T

S

P

L

E

S

M

G

F

W

E

A

D

R

A

P

A

G

Q

L

F

R

A

E

A

R

F

F

R

I

R

G

V

L

A

T

D

D

A

T

A

V

R

W

M

R

Q

V

R

R

Y

A

G

Y

G

G

D

D

C

F

Y

L

A

S

Y

Y

C

C

M

L

V

V

A

A

A

E

G

G

H

A

V

V

D

D

A

I

V

A

E

E

A

P

G

Q

L

V

K

S

A

V

Y

W

D
|
D

V

L

Q

A

A

A

L

L

I

D

P

I

I

V

V

V

Q

R

G

E

A

A

G

G

V

R

M

L

T

T

S

S

W

L

T

D

G

G

G

V

D

A

A

G

Q

P

Q

H

G

G

T

S

V

A

V

V

A

A

C

T

G

-

D

N

P

G

R

L

L

L

H

H

Q

D

Q

E

I

V

I

L

A

T

L

R

L

L

N

N

binding phosphate ion: E65 (= E74), I83 (= I92), D84 (= D93), G85 (= G94), T86 (= T95)
binding zinc ion: D42 (= D51), E65 (= E74), E65 (= E74), E66 (= E75), D81 (= D90), D81 (= D90), D211 (= D214)

Q9M8S8 Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Myo-inositol monophosphatase; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 85% coverage: 30:253/263 of query aligns to 29:260/271 of Q9M8S8

query
sites
Q9M8S8

F

F

R

Y

S

E

A

T

P

K

A

H

V

V

E

E

D

H

K

K

G

G

G

-

R

-

H

Q

F

V

D

D

P

L

V

V

T

T

A

E

A

T

D

D

K

K

A

G

A

C

E

E

R

E

A

L

M

V

R

F

D

N

L

H

I

L

L

K

A

Q

K

L

Y

F

P

P

E

N

H

H

G

K

I

F

L

I

G

G

E

E

E

T

D

T

R

A

V

A

A

F

G

G

T

V

S

T

P

E

L

L

T

T

-

D

-

E

-

P

-

T

W

W

V

I

L

V

D

D

P
|
P

I

L

D

D

G

G

T

T

R

T

A

N

F

F

I

V

T

H

G

G

L

F

P

P

L

F

W

V

G

C

T

V

L

S

I

I

A

G

L

L

N

T

D

I

G

G

Q

K

R

V

P

P

V

V

I

V

G

G

I

V

M

V

D

Y

Q

N

P

P

F

I

T

M

R

E

E

E

R

L

F

F

S

T

G

G

-

V

D

Q

G

G

S

K

R

G

A

A

W

F

L

L

N

N

G

G

Q

K

P

R

L

I

R

K

T

V

R

S

P

A

C

Q

A

S

D

E

L

L

A

L

Q

T

A

A

K

L

L

L

M

V

C

T

T

E

T

A

P

G

E

T

M

K

F

R

E

D

D

K

A

A

A

T

Q

L

F

D

A

D

A

T

F

T

R

N

R

R

V

I

A

N

D

S

-

L

-

T

A

K

A

V

R

R

M

S

Q

L

R

R

Y

M

G

S

G

G

D

S

C

C

-

A

Y

L

A

D

Y

L

C

C

M

G

V

V

A

A

A

C

G

G

H

R

V

V

D

D

A

I

V

F

V

Y

E

E

A

L

G

G

L

F

K

G

A

G

-

P

Y

W

D

D

V

I

Q

A

A

A

L

G

I

I

P

V

I

I

V

V

Q

K

G

E

A

A

G

G

V

L

M

I

T

F

S

D

W

P

T

S

G

G

G

K

D

D

A

L

Q

D

Q

I

G

T

G

S

T

Q

V

R

V

I

A

A

C

A

G

S

D

N

P

A

R

S

L

L

H

K

Q

E

P92 (= P91) mutation to L: In vtc4-1; low levels of ascorbate.

Q19420 Inositol monophosphatase ttx-7; IMP; IMPase; Abnormal thermotaxis protein 7; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase; EC 3.1.3.25; EC 3.1.3.94 from Caenorhabditis elegans (see paper)
29% identity, 89% coverage: 2:234/263 of query aligns to 5:250/285 of Q19420

query
sites
Q19420

P

P

L

I

T

H

A

E

E

E

Q

E

L

Q

I

V

E

-

Y

F

M

V

D

D

F

Y

A

A

A

I

G

E

L

L

A

V

Q

K

A

K

A

A

G

G

N

T

A

L

S

V

L

R

P

T

Y

A

F

F

R

D

S

S

A

P

P

E

A

S

V

K

E

V

D

D

K

T

G

K

G

S

R

S

H

N

F

T

D

D

P

L

V

V

T

T

A

E

A

T

D

D

K

Q

A

A

A

V

E

E

R

K

A

L

M

L

R

I

D

E

L

G

I

L

L

S

A

E

K

R

Y

F

P

K

E

G

H

H

G

R

I

F

L

I

G

G

E

E

E

S

-

V

-

A

-

G

D

A

R

K

V

I

A

E

G

W

T

T

S

D

P

A

L

P

T

T

W

W

V

I

L

I

D

D

P

P

I

I

D

D

G

G

T

T

R

T

A

N

F

F

I

V

T

H

G

R

L

I

P

P

L

M

W

I

G

A

T

I

L

C

I

V

A

G

L

L

N

A

D

I

G

K

Q

K

R

Q

P

I

V

R

I

A

G

G

I

I

M

V

D

Y

Q

N

P

P

F

I

T

T

R

N

E

E

R

L

F

Y

S

L

G

A

D

Q

-

L

G

G

S

K

R

G

A

A

W

F

L

K

N

N

G

G

Q

F

P

P

L

I

R

R

T

A

R

S

P

K

C

N

A

Q

D

L

L

L

A

S

Q

K

A

G

K

-

L

V

M

L

C

C

T

Q

T

S

P

L

E

G

M

L

F

H

E

N

-

R

-

V

-

Q

-

F

-

G

-

D

-

R

-

W

-

L

D

D

A

I

A

A

Q

Q

F

S

A

N

A

M

F

R

R

N

R

Q

V

V

A

M

D

A

A

G

A

V

R

R

M

G

Q

H

R

R

-

S

Y

F

G

G

G

S

D

A

C

A

Y

I

A

N

Y

M

C

V

M

M

V

V

A

A

A

Q

G
|
G

H

S

V

C

D

D

A

G

V

Y

V

V

E

E

A

Y

G

G

L

I

K

H

A

A

Y

W

D

D

V

V

Q

A

A

A

L

P

I

S

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

V

T

T

S

D

W

P

T

T

G

G

G216 (= G200) mutation to E: In nj40; induces defects in thermotaxis.

2bjiA High resolution structure of myo-inositol monophosphatase, the target of lithium therapy (see paper)
30% identity, 86% coverage: 10:235/263 of query aligns to 5:239/274 of 2bjiA

query
sites
2bjiA

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

G

A

Q

A

A

G

G

N

E

A

V

S

V

L

R

P

E

Y

A

F

L

R

K

S

N

A

E

P

M

A

N

V

I

E

M

D

V

K

K

G

S

R

P

H

A

F

-

D

D

P

L

V

V

T

T

A

A

A

T

D

D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

T

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

R

T

A

N

F

F

I

V

T

H

G

G

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

V

D

V

G

N

Q

K

R

K

P

M

V

E

I

F

G

G

I

I

M

V

D

Y

Q

S

P

C

F

L

T

E

R

D

E

K

R

M

F

Y

S

T

G

G

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L

C

N

N

G

G

Q

Q

P

K

L

L

R

Q

T

V

R

S

P

H

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T

E

-

L

-

G

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

I

A

I

A

L

Q

S

F

N

A

I

A

E

F

R

R

L

V

C

A

L

D

P

A

I

A

H

R

G

M

I

Q

R

R

G

Y

V

G

G

G

T

D

A

C

A

Y

L

A

N

Y

M

C

C

M

L

V

V

A

A

G

G

H

A

V

A

D

D

A

A

V

Y

E

E

A

M

G

G

L

I

K

H

A

C

Y

W

D
|
D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

L

S

D

W

V

T

T

G

G

active site: D88 (= D90), I90 (= I92), D91 (= D93), D218 (= D214)
binding magnesium ion: E68 (= E74), D88 (= D90), D88 (= D90), I90 (= I92), D91 (= D93), D218 (= D214)

P20456 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Bos taurus (Bovine) (see paper)
30% identity, 86% coverage: 10:235/263 of query aligns to 7:241/277 of P20456

query
sites
P20456

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

G

A

Q

A

A

G

G

N

E

A

V

S

V

L

R

P

E

Y

A

F

L

R

K

S

N

A

E

P

M

A

N

V

I

E

M

D

V

K

K

G

S

R

P

H

A

F

-

D

D

P

L

V

V

T

T

A

A

A

T

D

D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

T

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

R

T

A

N

F

F

I

V

T

H

G

G

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

V

D

V

G

N

Q

K

R

K

P

M

V

E

I

F

G

G

I

I

M

V

D

Y

Q

S

P

C

F

L

T

E

R

D

E

K

R

M

F

Y

S

T

G

G

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L

C

N

N

G

G

Q

Q

P

K

L

L

R

Q

T

V

R

S

P

H

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T

E

-

L

-

G

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

I

A

I

A

L

Q

S

F

N

A

I

A

E

F

R

R

L

V

C

A

L

D

P

A

I

A

H

R

G

M

I

Q

R

R

G

Y

V

G

G

G

T

D

A

C

A

Y

L

A

N

Y

M

C

C

M

L

V

V

A

A

G

G

H

A

V

A

D

D

A

A

V

Y

E

E

A

M

G

G

L

I

K

H

A

C

Y

W

D
|
D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

L

S

D

W

V

T

T

G

G

E70 (= E74) binding
D90 (= D90) binding ; binding
I92 (= I92) binding
D93 (= D93) binding
D220 (= D214) binding

2hhmA Structure of inositol monophosphatase, the putative target of lithium therapy (see paper)
29% identity, 86% coverage: 10:235/263 of query aligns to 3:237/272 of 2hhmA

query
sites
2hhmA

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

R

A

Q

A

A

G

G

N

E

A

V

S

V

L

C

P

E

Y

A

F

I

R

K

S

N

A

E

P

M

A

N

V

V

E

M

D

L

K

K

G

S

R

-

H

P

F

V

D

D

P

L

V

V

T

T

A

A

A

T

D
|
D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

S

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

R

T

A

N

F

F

I

V

T

H

G

R

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

A

D

V

G

N

Q

K

R

K

P

I

V

E

I

F

G

G

I

V

M

V

D

Y

Q

S

P

C

F

V

T

E

R

G

E

K

R

M

F

Y

S

T

G

A

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L

C

N

N

G

G

Q

Q

P

K

L

L

R

Q

T

V

R

S

P

Q

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T

E

-

L

-

G

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

M

A

V

A

L

Q

S

F

N

A

M

A

E

F

K

R

L

R

F

V

C

A

I

D

P

A

V

A

H

R

G

M

I

Q

R

R

S

Y

V

G

G

G

T

D

A

C

A

Y

V

A

N

Y

M

C

C

M

L

V

V

A

A

A

T

G

G

H

G

V

A

D

D

A

A

V

Y

E

E

A

M

G

G

L

I

K

H

A

C

Y

W

D
|
D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

M

S

D

W

V

T

T

G

G

active site: D43 (= D51), E66 (= E74), D86 (= D90), I88 (= I92), D89 (= D93), T91 (= T95), D216 (= D214)
binding sulfate ion: E66 (= E74), D86 (= D90), D89 (= D93), G90 (= G94), T91 (= T95)

1imbA Structural analysis of inositol monophosphatase complexes with substrates (see paper)
29% identity, 86% coverage: 10:235/263 of query aligns to 3:237/272 of 1imbA

query
sites
1imbA

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

R

A

Q

A

A

G

G

N

E

A

V

S

V

L

C

P

E

Y

A

F

I

R

K

S

N

A

E

P

M

A

N

V

V

E

M

D

L

K

K

G

S

R

-

H

P

F

V

D

D

P

L

V

V

T

T

A

A

A

T

D
|
D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

S

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

R

T

A

N

F

F

I

V

T

H

G

R

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

A

D

V

G

N

Q

K

R

K

P

I

V

E

I

F

G

G

I

V

M

V

D

Y

Q

S

P

C

F

V

T

E

R

G

E

K

R

M

F

Y

S

T

G

A

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L

C

N

N

G

G

Q

Q

P

K

L

L

R

Q

T

V

R

S

P

Q

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T

E

-

L

-

G

-
x
S

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

M

A

V

A

L

Q

S

F

N

A

M

A

E

F

K

R

L

R

F

V

C

A

I

D

P

A

V

A

H

R

G

M

I

Q

R

R

S

Y

V

G

G

G
x
T

D
x
A

C

A

Y

V

A

N

Y

M

C

C

M

L

V

V

A

A

A

T

G

G

H

G

V

A

D

D

A

A

V

Y

E
|
E

A

M

G

G

L

I

K

H

A

C

Y

W

D
|
D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

M

S

D

W

V

T

T

G

G

active site: D43 (= D51), E66 (= E74), D86 (= D90), I88 (= I92), D89 (= D93), T91 (= T95), D216 (= D214)
binding l-myo-inositol-1-phosphate: D89 (= D93), G90 (= G94), S161 (vs. gap), T191 (≠ G189), A192 (≠ D190), E209 (= E207), D216 (= D214)

1awbA Human myo-inositol monophosphatase in complex with d-inositol-1- phosphate and calcium
29% identity, 86% coverage: 10:235/263 of query aligns to 3:237/272 of 1awbA

query
sites
1awbA

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

R

A

Q

A

A

G

G

N

E

A

V

S

V

L

C

P

E

Y

A

F

I

R

K

S

N

A

E

P

M

A

N

V

V

E

M

D

L

K

K

G

S

R

-

H

P

F

V

D

D

P

L

V

V

T

T

A

A

A

T

D
|
D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

S

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

R

T

A

N

F

F

I

V

T

H

G

R

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

A

D

V

G

N

Q

K

R

K

P

I

V

E

I

F

G

G

I

V

M

V

D

Y

Q

S

P

C

F

V

T

E

R

G

E

K

R

M

F

Y

S

T

G

A

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L

C

N

N

G

G

Q

Q

P

K

L

L

R

Q

T

V

R

S

P

Q

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T
x
E

-

L

-

G

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

M

A

V

A

L

Q

S

F

N

A

M

A

E

F

K

R

L

R

F

V

C

A

I

D

P

A

V

A

H

R

G

M

I

Q

R

R

S

Y

V

G
|
G

G
x
T

D
x
A

C

A

Y

V

A

N

Y

M

C

C

M

L

V

V

A

A

A

T

G

G

H

G

V

A

D

D

A

A

V

Y

E
|
E

A

M

G

G

L

I

K

H

A

C

Y

W

D
|
D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

M

S

D

W

V

T

T

G

G

active site: D43 (= D51), E66 (= E74), D86 (= D90), I88 (= I92), D89 (= D93), T91 (= T95), D216 (= D214)
binding calcium ion: E66 (= E74), D86 (= D90), D86 (= D90), I88 (= I92), D89 (= D93), D216 (= D214)
binding d-myo-inositol-1-phosphate: D86 (= D90), D89 (= D93), G90 (= G94), E158 (≠ T161), G190 (= G188), T191 (≠ G189), A192 (≠ D190), E209 (= E207)

6zk0AAA human impase with ebselen (see paper)
29% identity, 86% coverage: 10:235/263 of query aligns to 4:238/274 of 6zk0AAA

query
sites
6zk0AAA

E

E

Y

C

M

M

D

D

F

Y

A

A

A

V

G

T

L

L

A

A

Q

R

A

Q

A

A

G

G

N

E

A

V

S

V

L

C

P

E

Y

A

F

I

R

K

S

N

A

E

P

M

A

N

V

V

E

M

D

L

K

K

G

S

R

-

H

P

F

V

D

D

P

L

V

V

T

T

A

A

A

T

D

D

K

Q

A

K

A

V

E

E

R

K

A

M

M

L

R

I

D

S

L

S

I

I

L

K

A

E

K

K

Y

Y

P

P

E

S

H

H

G

S

I

F

L

I

G

G

E
|
E

E

E

-

S

-

V

-

A

-

G

E

E

D

K

R

S

V

I

A

L

G

T

T

D

S

N

P

P

L

-

T

T

W

W

V

I

L

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

R

T

A

N

F

F

I

V

T

H

G

R

L

F

P

P

L

F

W

V

G

A

T

V

L

S

I

I

A

G

L

F

N

A

D

V

G

N

Q

K

R

K

P

I

V

E

I

F

G

G

I

V

M

V

D

Y

Q

S

P

C

F

V

T

E

R

G

E
x
K

R

M

F

Y

S

T

G

A

-

R

D

K

G

G

S

K

R

G

A

A

W

F

L
x
C

N
|
N

G

G

Q
|
Q

P

K

L

L

R

Q

T

V

R

S

P

Q

C

Q

A

E

D

D

L

I

A

T

Q

K

A

S

K

L

L

L

M

V

C

T

T

E

-

L

-

G

-

S

-

R

T

T

P

P

E

E

M

T

F

V

E

R

D

M

A

V

A

L

Q

S

F

N

A

M

A

E

F

K

R

L

R

F

V

C

A

I

D

P

A

V

A

H

R

G

M

I

Q

R

R

S

Y

V

G

G

G

T

D

A

C

A

Y

V

A

N

Y

M

C

C

M

L

V

V

A

A

A

T

G

G

H

G

V

A

D

D

A

A

V

Y

E

E

A

M

G

G

L

I

K

H

A

C

Y

W

D

D

V

V

Q

A

A

G

L

A

I

G

P

I

I

I

V

V

Q

T

G

E

A

A

G

G

V

V

M

L

T

M

S

D

W

V

T

T

G

G

binding N-phenyl-2-selanylbenzamide: K126 (≠ E129), C138 (≠ L140), N139 (= N141), Q141 (= Q143)
binding manganese (ii) ion: E67 (= E74), D87 (= D90), I89 (= I92), D90 (= D93)

Query Sequence

>RR42_RS03715 FitnessBrowser__Cup4G11:RR42_RS03715
MPLTAEQLIEYMDFAAGLAQAAGNASLPYFRSAPAVEDKGGRHFDPVTAADKAAERAMRD
LILAKYPEHGILGEEEDRVAGTSPLTWVLDPIDGTRAFITGLPLWGTLIALNDGQRPVIG
IMDQPFTRERFSGDGSRAWLNGQPLRTRPCADLAQAKLMCTTPEMFEDAAQFAAFRRVAD
AARMQRYGGDCYAYCMVAAGHVDAVVEAGLKAYDVQALIPIVQGAGGVMTSWTGGDAQQG
GTVVACGDPRLHQQIIALLNAPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory