SitesBLAST
Comparing RR42_RS04385 FitnessBrowser__Cup4G11:RR42_RS04385 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
25% identity, 96% coverage: 3:407/420 of query aligns to 4:348/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (≠ V6), G8 (= G7), G10 (= G9), V11 (= V10), I12 (= I11), V30 (≠ L29), E31 (= E30), K32 (≠ R31), E38 (= E38), A39 (≠ T39), S40 (= S40), A43 (≠ N43), G45 (= G45), L46 (≠ Q46), V171 (= V225), G200 (= G254), G201 (= G255), W203 (≠ G257), G298 (= G358), R300 (= R360), P301 (= P361), Y326 (≠ G385), R327 (≠ A386), N328 (≠ L387), G329 (= G388), I330 (≠ F389)
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
29% identity, 67% coverage: 2:283/420 of query aligns to 6:233/830 of Q9AGP8
- IV 14:15 (≠ VI 10:11) binding
- DQ 35:36 (≠ ER 30:31) binding
- STSH 45:48 (≠ VLAK 60:63) binding
- L52 (≠ W67) binding
- V174 (= V225) binding
- H225 (≠ K275) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
Sites not aligning to the query:
- 259 Important for catalytic activity; binding ; Y→F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- 360:363 binding
- 539 binding
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
29% identity, 67% coverage: 2:283/420 of query aligns to 3:230/827 of 3gsiA
- active site: H222 (≠ K275)
- binding flavin-adenine dinucleotide: G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (≠ E30), Q33 (≠ R31), G41 (vs. gap), S42 (≠ V60), T43 (≠ L61), H45 (≠ K63), P47 (= P65), L49 (≠ W67), T170 (≠ S224), V171 (= V225), A200 (≠ G254), G201 (= G255), W203 (≠ G257), H222 (≠ K275)
Sites not aligning to the query:
- active site: 256, 549
- binding flavin-adenine dinucleotide: 256, 330, 331, 332, 357, 358, 359, 360
- binding magnesium ion: 254, 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
29% identity, 67% coverage: 2:283/420 of query aligns to 3:230/827 of 1pj7A
- active site: H222 (≠ K275)
- binding flavin-adenine dinucleotide: G8 (= G7), G10 (= G9), I11 (≠ V10), V12 (≠ I11), D32 (≠ E30), Q33 (≠ R31), G41 (vs. gap), S42 (≠ V60), T43 (≠ L61), H45 (≠ K63), P47 (= P65), L49 (≠ W67), T170 (≠ S224), V171 (= V225), A200 (≠ G254), G201 (= G255), W203 (≠ G257), H222 (≠ K275)
Sites not aligning to the query:
- active site: 256, 549
- binding flavin-adenine dinucleotide: 256, 331, 357, 358, 359, 360
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
29% identity, 67% coverage: 2:283/420 of query aligns to 4:231/828 of 1pj6A
- active site: H223 (≠ K275)
- binding flavin-adenine dinucleotide: G9 (= G7), G11 (= G9), I12 (≠ V10), V13 (≠ I11), D33 (≠ E30), Q34 (≠ R31), G42 (vs. gap), S43 (≠ V60), T44 (≠ L61), H46 (≠ K63), P48 (= P65), L50 (≠ W67), V172 (= V225), A201 (≠ G254), G202 (= G255), W204 (≠ G257), H223 (≠ K275)
Sites not aligning to the query:
6j39A Crystal structure of cmis2 with inhibitor (see paper)
28% identity, 72% coverage: 108:410/420 of query aligns to 58:359/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: R243 (= R288), Y252 (≠ I301), Y267 (≠ A316), R308 (= R360), R334 (≠ A386), I335 (≠ L387)
- binding flavin-adenine dinucleotide: A174 (≠ G222), A203 (≠ G254), W206 (≠ G257), I228 (≠ S278), Y252 (≠ I301), R308 (= R360), S333 (≠ G385), R334 (≠ A386), I335 (≠ L387), G336 (= G388), V337 (≠ F389), Q338 (≠ T390)
Sites not aligning to the query:
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46, 49
- binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44
6j38A Crystal structure of cmis2 (see paper)
28% identity, 72% coverage: 108:410/420 of query aligns to 58:359/368 of 6j38A
- binding flavin-adenine dinucleotide: A174 (≠ G222), A203 (≠ G254), W206 (≠ G257), G226 (= G276), G306 (= G358), R308 (= R360), S333 (≠ G385), R334 (≠ A386), I335 (≠ L387), G336 (= G388), V337 (≠ F389), Q338 (≠ T390)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44
Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
32% identity, 40% coverage: 105:272/420 of query aligns to 78:242/824 of Q8GAI3
Sites not aligning to the query:
- 66 mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
- 67 H→A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.
2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
22% identity, 72% coverage: 115:416/420 of query aligns to 83:383/403 of 2gagB
- binding flavin-adenine dinucleotide: V195 (= V225), G224 (= G254), A225 (≠ G255), H227 (≠ G257), L231 (= L261), L246 (≠ I279), G352 (= G385), T353 (≠ A386), G354 (≠ L387), G355 (= G388), F356 (= F389), K357 (≠ T390)
- binding flavin mononucleotide: H171 (≠ C200), V250 (≠ I283), E278 (≠ N308), R321 (≠ Q354), W323 (= W356)
- binding 2-furoic acid: M263 (≠ D296), Y270 (= Y303), K357 (≠ T390)
- binding sulfite ion: K170 (≠ D199), K276 (≠ I306)
Sites not aligning to the query:
- active site: 61, 64, 65
- binding flavin-adenine dinucleotide: 26, 28, 29, 30, 51, 52, 58, 59, 60, 62, 63, 64, 66
- binding flavin mononucleotide: 61, 62
- binding 2-furoic acid: 64, 66, 68, 401
Q50LF2 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain U-96) (see 4 papers)
22% identity, 69% coverage: 129:416/420 of query aligns to 99:385/405 of Q50LF2
- K172 (≠ D199) mutation to A: Retains 39% of wild-type activity.; mutation to D: Retains 32% of wild-type activity.; mutation to R: Retains 58% of wild-type activity.
- H173 (≠ C200) modified: Tele-8alpha-FMN histidine
- V197 (= V225) binding
- H270 (≠ I301) mutation to A: 10-fold decrease in catalytic efficiency.
- Y272 (= Y303) mutation to A: 13000-fold decrease in catalytic efficiency.; mutation to F: 130-fold decrease in catalytic efficiency.
- G354 (= G385) binding
- G357 (= G388) binding
- K359 (≠ T390) binding ; mutation K->A,D: Loss of activity.; mutation to R: Retains 0.07% of wild-type activity. Shows higher apparent KM for sarcosine.
Sites not aligning to the query:
- 31 binding
- 32 binding
- 53 binding
- 61 binding
- 62 binding
- 66 binding
- 68 binding
3ad8B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with pyrrole 2-carboxylate (see paper)
22% identity, 69% coverage: 129:416/420 of query aligns to 98:384/404 of 3ad8B
- active site: G326 (= G358), K358 (≠ T390)
- binding flavin-adenine dinucleotide: V196 (= V225), G225 (= G254), A226 (≠ G255), H228 (≠ G257), L247 (≠ I279), G353 (= G385), T354 (≠ A386), G355 (≠ L387), G356 (= G388), F357 (= F389), K358 (≠ T390)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I283), E279 (≠ N308), R322 (≠ Q354), W324 (= W356)
- binding pyrrole-2-carboxylate: M264 (≠ D296), Y271 (= Y303), T354 (≠ A386), K358 (≠ T390)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding pyrrole-2-carboxylate: 65, 67, 69, 402
3ad7B Heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with methylthio acetate (see paper)
22% identity, 69% coverage: 129:416/420 of query aligns to 98:384/404 of 3ad7B
- active site: G326 (= G358), K358 (≠ T390)
- binding flavin-adenine dinucleotide: V196 (= V225), G225 (= G254), A226 (≠ G255), H228 (≠ G257), L247 (≠ I279), G353 (= G385), T354 (≠ A386), G355 (≠ L387), G356 (= G388), F357 (= F389), K358 (≠ T390)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I283), K277 (≠ I306), E279 (≠ N308), R322 (≠ Q354), W324 (= W356)
- binding [methylthio]acetate: M264 (≠ D296), Y271 (= Y303), T354 (≠ A386), K358 (≠ T390)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
- binding [methylthio]acetate: 67, 69, 402
1vrqB Crystal structure of heterotetrameric sarcosine oxidase from corynebacterium sp. U-96 in complex with folinic acid (see paper)
22% identity, 69% coverage: 129:416/420 of query aligns to 98:384/402 of 1vrqB
- active site: G326 (= G358), K358 (≠ T390)
- binding n,n-dimethylglycine: K358 (≠ T390)
- binding flavin-adenine dinucleotide: V196 (= V225), A224 (= A253), G225 (= G254), H228 (≠ G257), L247 (≠ I279), G353 (= G385), T354 (≠ A386), G355 (≠ L387), G356 (= G388), F357 (= F389), K358 (≠ T390)
- binding flavin mononucleotide: H172 (≠ C200), V251 (≠ I283), E279 (≠ N308), R322 (≠ Q354), W324 (= W356)
Sites not aligning to the query:
- active site: 62, 65, 66
- binding n,n-dimethylglycine: 65, 67, 69, 401
- binding flavin-adenine dinucleotide: 26, 27, 29, 30, 31, 51, 52, 53, 59, 60, 61, 63, 64, 65, 67
- binding flavin mononucleotide: 62, 63
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
27% identity, 45% coverage: 223:411/420 of query aligns to 172:354/369 of O31616
- V174 (= V225) binding
- H244 (= H291) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R360) binding
- 327:333 (vs. 384:390, 29% identical) binding
- R329 (≠ A386) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
26% identity, 45% coverage: 223:411/420 of query aligns to 172:354/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ S293), R302 (= R360), R329 (≠ A386)
- binding flavin-adenine dinucleotide: V174 (= V225), S202 (≠ G254), G203 (= G255), W205 (≠ G257), F209 (≠ L261), G300 (= G358), R302 (= R360), H327 (= H384), R329 (≠ A386), N330 (≠ L387), G331 (= G388), I332 (≠ F389)
- binding phosphate ion: R254 (≠ Y303)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
- binding phosphate ion: 89
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
26% identity, 45% coverage: 223:411/420 of query aligns to 172:354/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (≠ S224), V174 (= V225), S202 (≠ G254), G203 (= G255), W205 (≠ G257), F209 (≠ L261), G300 (= G358), R302 (= R360), H327 (= H384), F328 (≠ G385), R329 (≠ A386), N330 (≠ L387), G331 (= G388), I332 (≠ F389)
- binding glycolic acid: Y246 (≠ T295), R302 (= R360), R329 (≠ A386)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49
P40875 Sarcosine oxidase subunit beta; Sarcosine oxidase subunit B; Sarcosine oxidase (5,10-methylenetetrahydrofolate-forming) subunit beta; Tetrameric sarcosine oxidase subunit beta; TSOX subunit beta; EC 1.5.3.24 from Corynebacterium sp. (strain P-1) (see 3 papers)
22% identity, 69% coverage: 115:405/420 of query aligns to 85:374/405 of P40875
- C146 (≠ E176) mutation to S: No change in activity.
- H173 (≠ C200) modified: Tele-8alpha-FMN histidine; mutation to N: Prevents covalent attachment of FMN. Loss of activity. The mutant is considerably less stable than wild-type enzyme, the beta and delta subunits are lost during purification, which yields a stable alpha-gamma complex.
- H175 (≠ R202) mutation to A: No effect on FMN binding and activity.
- C195 (≠ T223) mutation to S: No change in activity.
- C351 (≠ I382) mutation to A: No change in activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 30 G→A: Prevents covalent attachment of FMN. Blocks subunit assembly.
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
25% identity, 76% coverage: 98:416/420 of query aligns to 52:366/377 of Q5L2C2
- V180 (= V225) binding
- R309 (= R360) binding
- 334:340 (vs. 384:390, 29% identical) binding
- R336 (≠ A386) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 76% coverage: 98:416/420 of query aligns to 51:364/370 of 4yshA
- active site: Q52 (≠ S99), I262 (≠ V312), L283 (= L333), G305 (= G358), N335 (≠ L387), L338 (≠ T390)
- binding flavin-adenine dinucleotide: V178 (= V225), S206 (≠ G254), G207 (= G255), W209 (≠ G257), R307 (= R360), H332 (= H384), R334 (≠ A386), N335 (≠ L387), G336 (= G388), I337 (≠ F389)
Sites not aligning to the query:
- active site: 45, 48, 49
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
25% identity, 76% coverage: 98:416/420 of query aligns to 51:364/368 of 4yshB
- active site: Q52 (≠ S99), I262 (≠ V312), L283 (= L333), G305 (= G358), N335 (≠ L387), L338 (≠ T390)
- binding flavin-adenine dinucleotide: V178 (= V225), S206 (≠ G254), W209 (≠ G257), R307 (= R360), H332 (= H384), R334 (≠ A386), N335 (≠ L387), G336 (= G388), I337 (≠ F389), L338 (≠ T390)
- binding glycine: G249 (≠ I301), Y251 (= Y303), Y251 (= Y303), A264 (≠ G314), R307 (= R360), R334 (≠ A386), R334 (≠ A386)
Sites not aligning to the query:
- active site: 45, 48, 49
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
Query Sequence
>RR42_RS04385 FitnessBrowser__Cup4G11:RR42_RS04385
MKVVIVGAGVIGMSAAWRLAGDGHEVTVLERRDGPGEETSFANGGQLSYSYVAPLAGPGV
LAKVPGWLLRADSPMRFRPSADPAQWRWLAAFARACNASASEAATRKLLRLAFYSRDLMQ
AFVDSQPADFGFARRGKLIVHRDAASFAAACRLLDYQASLGCEQQALDGDACVALEPALA
GIRGAIAGAIHTPSEEVADCHRFCLALAQLLHDRPGVSLRFGTSVLGIEREGDRVSGVRT
DAGIERADAVVVAGGIGSVALLKPLGVQAPLWPLKGYSITVPIRDGERAPHISITDAARK
IVYARIGNTLRVAGMADLVRADTNIDRARIATLACETRTLFGGMAPGMEPEQLQPWAGLR
PATPTGLPMVGRSGVRGLWLNIGHGALGFTLAMGSAGLLADGLAGRKPAIDDTDFAAMLA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory